ABSTRACT
After depletion of intracellular Ca2+ stores the capacitative response triggers an extracellular Ca2+ influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca2+ depletion by a Ca2+-free extracellular solution followed by Ca2+ readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. In presence of 2-APB the magnitude of a second paired contraction by serotonin or histamine was significantly less than a first one, likely because 2-APB inhibited store refilling by capacitative Ca2+ entry. 2-APB inhibition of sarcoplasmic reticulum Ca2+ release was excluded because this blocker did not affect serotonin force development in a Ca2+-free solution. The PCR technique showed the presence of mRNAs for STIM proteins (1 and 2), for Orai proteins (1, 2 and 3) and for TRPC channels (subtypes 1, 3, 4 and 6) in the smooth muscle of the human umbilical artery. Hence, this artery presents a capacitative contractile response triggered by stimulation with physiological vasoconstrictors and expresses mRNAs for proteins and channels previously identified as SOCs.
Subject(s)
Humans , Boron Compounds/pharmacology , Muscle Contraction/drug effects , Muscle, Smooth/drug effects , Muscle, Smooth/metabolism , RNA, Messenger/genetics , Umbilical Arteries/drug effects , Vascular Capacitance/drug effects , Blotting, Western , Cells, Cultured , Calcium Channel Blockers/pharmacology , Calcium Channels/chemistry , Calcium Channels/genetics , Calcium Channels/metabolism , Calcium/metabolism , Histamine Agonists/pharmacology , Histamine/pharmacology , Membrane Proteins/genetics , Membrane Proteins/metabolism , Muscle, Smooth/cytology , Neoplasm Proteins/genetics , Neoplasm Proteins/metabolism , Real-Time Polymerase Chain Reaction , Reverse Transcriptase Polymerase Chain Reaction , Sarcoplasmic Reticulum/drug effects , Sarcoplasmic Reticulum/metabolism , Serotonin Receptor Agonists/pharmacology , Serotonin/pharmacology , TRPC Cation Channels/genetics , TRPC Cation Channels/metabolism , Umbilical Arteries/cytology , Umbilical Arteries/metabolismABSTRACT
OBJETIVO: avaliar o efeito do sulfato de magnésio sobre o índice de pulsatilidade (IP) das artérias uterinas, umbilicais e cerebral média fetal, de acordo com a persistência ou não da incisura protodiastólica bilateral das artérias uterinas na pré-eclâmpsia grave. MÉTODOS: foi desenvolvido um estudo do tipo coorte, incluindo 40 gestantes com pré-eclâmpsia grave, das quais 23 apresentavam incisura protodiastólica bilateral e 17, incisura ausente/unilateral. As pacientes foram submetidas a doplervelocimetria antes e depois de 20 minutos da administração intravenosa de 6 g do sulfato de magnésio. O exame foi realizado com a paciente em posição semi-Fowler, obtendo-se os sonogramas durante a inatividade fetal, em períodos de apneia e ausência de contrações uterinas. Todos os exames foram realizados por dois pesquisadores, considerando a média como resultado final. A comparação dos IP antes e depois do sulfato de magnésio em cada grupo foi realizada pelo teste de Wilcoxon. A diferença das duas medidas (antes e depois do sulfato de magnésio) foi comparada entre os grupos (incisura bilateral e incisura ausente/unilateral) utilizando-se o teste de Mann-Whitney. RESULTADOS: houve um aumento significativo da frequência cardíaca materna e uma diminuição da pressão arterial materna e da mediana dos IP das duas artérias uterinas e da artéria cerebral média fetal depois da utilização do sulfato de magnésio em ambos os grupos. Houve redução significativa do IP da artéria uterina esquerda e da artéria umbilical apenas no grupo com incisura protodiastólica unilateral/ausente. No entanto, não foram encontradas diferenças significativas em relação ao IP da artéria uterina direita e relação umbilical/cerebral antes e depois do sulfato de magnésio em cada grupo. Não se encontrou diferença entre os grupos, antes e depois do sulfato de magnésio, para nenhum dos desfechos estudados. CONCLUSÕES: após a administração intravenosa de 6 g do sulfato de magnésio nas...
PURPOSE: to evaluate the effect of magnesium sulphate on the pulsatility index (PI) of the uterine, umbilical and fetal middle cerebral arteries, according to the persistency or not of the bilateral protodiastolic notch of the uterine arteries in severe pre-eclampsia. METHODS: a cohort study including 40 pregnant women with severe pre-eclampsia, 23 of them presenting bilateral protodiastolic notch, and 17, unilateral/absent notch. The patients were submitted to Doppler velocimetry before and 20 minutes after the intravenous administration of 6 g of magnesium sulphate. The examination was carried out with the patient in semi-Fowler position, the sonograms being obtained during fetal inactivity, in apnea and absent uterine contraction periods. All the exams were performed by two researchers, the average being considered as the final result. Wilcoxon's test was used to compare the PI, before and after magnesium sulphate in both groups. The difference between the two measurements (before and after magnesium sulphate) was compared between the groups (bilateral incision and unilateral/absent incision) using the Mann-Whitney test. RESULTS: there was a significant increase in the maternal heart rate (MHR) and decrease in the maternal blood pressure, and in the PI medians of the two uterine arteries and in the fetal middle cerebral artery, after magnesium sulphate in both groups. There was a significant decrease in the PI of the left uterine artery and in the umbilical artery, only in the protodiastolic unilateral/absent notch group. Nevertheless, it was not found any significant difference regarding the PI of the right uterine artery, or the cerebral/umbilical relationship, before and after magnesium sulphate in each group. No difference between the groups was found, before and after magnesium sulphate, for any of the studied outcomes. CONCLUSIONS: after the intravenous administration of 6 g of magnesium sulphate to patients with severe pre-eclampsia...
Subject(s)
Adult , Female , Humans , Pregnancy , Anticonvulsants/pharmacology , Diastole , Magnesium Sulfate/pharmacology , Middle Cerebral Artery/drug effects , Middle Cerebral Artery/physiopathology , Pre-Eclampsia/physiopathology , Umbilical Arteries/drug effects , Umbilical Arteries/physiopathology , Uterus/blood supply , Uterus/drug effects , Cohort Studies , Pulse , Severity of Illness IndexABSTRACT
El siguiente trabajo tuvo por objetivo valorar las modificaciones producidas por la anestesia peridural, mediante el efecto Doppler, sobre la circulación de ambas arterias uterinas y arteria umbilical. Fue realizado en la Maternidad del Hospital D. F. Santojanni en el período comprendido entre octubre de 1993 y agosto de 1994. Se seleccionaron 33 pacientes con embarazos de término y 2 cesáreas anteriores, sin complicaciones médicas y/o quirúrgicas durante la gestación. Se efectuó la Velocimetría Doppler de arteria uterina derecha e izquierda y arteria umbilical con Doppler pulsado previa a la anestesia, 5 y 15 minutos después. Se calculó el cociente S/D. Los resultados mostraron a pesar de no encontrarse modificaciones significativas de la tensión arterial materna, un aumento del índice S/D a los 5 minutos en las arterias estudiadas sugiriendo una disminución del flujo útero-placentario y del flujo umbilical. Estos valores vuelven a estados preanestésicos excepto en arteria uterina izquierda. Pensamos que los 5 primeros minutos son críticos para los mecanismos de regulación hemodinámica maternos, motivo por el cual consideramos que aumentan los valores del índice S/D
Subject(s)
Humans , Female , Pregnancy , Anesthesia, Epidural/adverse effects , Placental Circulation , Doppler Effect , Umbilical Arteries/drug effects , Cesarean Section/trends , Placental Circulation/physiology , Laser-Doppler Flowmetry , Lidocaine/adverse effects , Lidocaine/therapeutic use , Umbilical Arteries/physiopathology , Vascular Resistance/drug effects , Vascular Resistance/physiologyABSTRACT
Según metodología descripta en trabajos anteriores, se estudia la contractilidad "in vitro" de los vasos coriales de 30 placentas humanas normales, y el efecto provocado por el aumento del PO**2 en el medio perfusor a expensas de concentraciones de H**2 O**2 de 1.25, 2.5 y 5.0 mM. El H**2 O**2 provoca una respuesta contráctil monofásica; la intensidad de la contraccíon aumenta proporcionalmente a la concentración de H**2 O**2 (F = 8.9 p > 0,01). La indometacina y la aspirina a concentraciones de 2.5 micronM y 0.25 mM respectivamente, inhiben aquella respuesta