ABSTRACT
The effects of different pesticides (propiconazole, profenofos, pretilachlor) on vermicomposting were evaluated for the adverse effects on soil enzyme activities (dehydrogenase, phosphatase, urease) and total microbial counts (TMC). There were remarkable increase in enzyme activities and TMC in presence of earthworm compared to control (earthworm absent). In comparison to control, the activities of phosphatase, dehydrogenase and urease in presence of vermicompost increased upto 30, 128 and 31.3% respectively; whereas increase of TMC was 71.9%. But, in presence of each of the pesticide, said activities decreased. Maximum inhibition of soil phosphatase activity (46.6%) was observed in presence of propiconazole (100 mg kg(-1)) after 120 days. Profenofos affected the soil dehydrogenase activity in the tune of 47% at 1000 mg kg(-1) concentration after 80 days and thereafter, the extent of toxicity decreased little. Soil urease activity was affected markedly in presence of profenofos and was 62% at 1000 mg kg(-1) level after 80 days. TMC also declined in presence of profenofos and pretilachlor. Increase in TMC was about 71.9% compared to control and the inhibition was more or less 60% when profenofos (1000 mg kg(-1)) was present even after 120 days of treatment.
Subject(s)
Agriculture , Bacteria/growth & development , Oxidoreductases/pharmacology , Pesticides/poisoning , Phosphoric Monoester Hydrolases/pharmacology , Population Dynamics , Refuse Disposal , Soil Microbiology , Soil Pollutants/poisoning , Urease/pharmacologyABSTRACT
El test respiratorio utilizando urea marcada con C es un método no invasivo empleado desde 1988 para detectar la presencia de Helicobacter pylori en pacientes portadores de enfermedad ácido péptica. Este método ha demostrado una sensibilidad del 90,2 por ciento y especificidad de 83,8 por ciento . En el Centro de Medicina Nuclear de Guanabara fueron realizados 1.200 test respiratorios, de los cuales el 67 por ciento se reportaron positivos, realizándose nuevos controles después del tratamiento médico, con resultados negativos
Subject(s)
Humans , Breath Tests , Gastritis/etiology , Helicobacter Infections/diagnosis , Helicobacter pylori/isolation & purification , Carbon Radioisotopes , Carbon Dioxide , Stomach/microbiology , Ethanolamines , Gamma Cameras , Gamma Cameras/instrumentation , Gamma Cameras/standards , Gastritis/diagnosis , Helicobacter pylori/physiology , Gastroesophageal Reflux/microbiology , Peptic Ulcer/diagnosis , Peptic Ulcer/etiology , Urease , Urease/metabolism , Urease/pharmacologyABSTRACT
O autor revê seus trabalhos anteriores sobre dosagem de uréia pela urease. Descreve método simples e seguro utilizando fontes alternativas de urease (Citrullus vulgaris, Soja hyspida e Canavalia ensiformes), utilizando apenas 10 microlitros de amostra. A amônia resultante é dosada fotometricamente pela formaçäo de azul de indofenol (reaçäo de Berthelot-Thomas modificada pelo Autor)