ABSTRACT
Abstract Urokinase-type plasminogen activator (uPA) and its receptor (uPAR) act in the proteolysis of basement membrane and extracellular matrix structures, facilitating tumor invasion. The purpose of this study was to evaluate the relationship between these proteins and clinicopathological parameters in squamous cell carcinoma of the oral tongue (SCCOT). Sixty cases of SCCOT were submitted to immunohistochemistry and analyzed semiquantitatively at the invasion front and in the tumor core. The results were associated with lymph node metastasis, clinical stage, locoregional recurrence, clinical outcome and histological grade of malignancy. A higher expression of uPA was observed in cases of tumors of high-grade versus low-grade malignancy (p = 0.010). Moreover, the cases with the worst pattern of invasion presented an overexpression of uPA (p = 0.011). The presence of locoregional recurrence was associated with uPAR (p = 0.039), and the expression of both biomarkers was much higher at the invasion front than in the tumor core (p < 0.001). The results suggest uPA and uPAR are involved in the progression and aggressiveness of SCCOT, mainly at the tumor-host interface.
Subject(s)
Humans , Male , Female , Tongue Neoplasms/chemistry , Carcinoma, Squamous Cell/chemistry , Urokinase-Type Plasminogen Activator/analysis , Receptors, Urokinase Plasminogen Activator/analysis , Reference Values , Tongue Neoplasms/pathology , Immunohistochemistry , Carcinoma, Squamous Cell/pathology , Biomarkers, Tumor/analysis , Risk Factors , Statistics, Nonparametric , Neoplasm Grading , Middle Aged , Neoplasm Invasiveness , Neoplasm Recurrence, Local/pathology , Neoplasm Recurrence, Local/chemistry , Neoplasm StagingABSTRACT
No abstract available.
Subject(s)
Female , Humans , Male , Glomerulonephritis, IGA/enzymology , Plasminogen Activator Inhibitor 1/analysis , Podocytes/enzymology , Receptors, Urokinase Plasminogen Activator/analysis , Urokinase-Type Plasminogen Activator/analysisABSTRACT
BACKGROUND/AIMS: The purpose of this study was to investigate the expression of urokinase-type plasminogen activator (uPA), uPA receptor (uPAR), and plasminogen activator inhibitor (PAI)-1 on podocytes in immunoglobulin A (IgA) glomerulonephritis (GN). METHODS: Renal biopsy specimens from 52 IgA GN patients were deparaffinized and subjected to immunohistochemical staining for uPA, PAI-1, and uPAR. The biopsies were classified into three groups according to the expression of uPA and uPAR on podocytes: uPA, uPAR, and a negative group. The prevalences of the variables of the Oxford classification for IgA GN were compared among the groups. RESULTS: On podocytes, uPA was positive in 11 cases and uPAR was positive in 38 cases; by contrast, PAI-1 was negative in all cases. Expression of both uPA and uPAR on podocytes was less frequently accompanied by tubulointerstitial fibrosis. CONCLUSIONS: Our results suggest a possible protective effect of podocyte uPA/uPAR expression against interstitial fibrosis.
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Atrophy , Biomarkers/analysis , Biopsy , Fibrosis , Glomerulonephritis, IGA/diagnosis , Immunohistochemistry , Plasminogen Activator Inhibitor 1/analysis , Podocytes/enzymology , Receptors, Urokinase Plasminogen Activator/analysis , Urokinase-Type Plasminogen Activator/analysisABSTRACT
Plasma bovino tratado com sulfato de celulose produz, em placas de fibrina, múltiplas formas de ativadores de plasminogênio. A fraçäo fibrinoliticamente mais ativa foi isolada por eletroforese preparativa. Testes amidolíticos com substratos cromogênicos e testes de imunodifusäo radial com anticorpos monoclonais revelaram que o ativador de plasminogênio isolado neste trabalho possui propriedades semelhantes às da uroquinase