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1.
Pensar mov ; 21(1)jun. 2023.
Article in English | LILACS, SaludCR | ID: biblio-1521283

ABSTRACT

León, F., Mestre. A., Priego, L., & Vera, J.C. (2023). Full study of Morphological adaptations in response to chronic exercise across musculoskeletal tissues: a systematic review. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 21(1), 1-21. To date, there is no systematic review that summarizes the morphological adaptations of the musculoskeletal system in response to chronic exercise. This systematic review selected original articles published in English between 2000 and 2020, with a clear exercise intervention and presenting a morphological change in the tissue under study, and covering human participants irrespective of age, gender or health condition. In total, 2819 records were identified. After removal of duplicates, title and abstract screening and full-text review, 67 records were included in the final analysis (6 for inter-vertebral disc, 6 for cartilage, 36 for bone, 2 for ligament, 9 for tendon and 7 for muscle). The most used interventions were aerobic, resistance, and plyometric exercise. Population ranged from children and healthy active people to individuals with a health condition. In conclusion, as a response to chronic exercise there are morphological adaptations in the tissues of the musculoskeletal system which vary from increased stiffness to an increase in cross-sectional area. Although tissues can adapt, several questions still linger, such as optimal dose and type of exercise, whether adaptations can occur in an injured tissue, and functional implications of these adaptations. Future research should address these questions.


León, F., Mestre. A., Priego, L. y Vera, J.C. (2023). Estudio complete de Adaptaciones morfológicas en respuesta al ejercicio crónico en los tejidos osteomusculares: una revisión sistemática. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 21(1), 1-21. Hasta la fecha, no existe una revisión sistemática que resuma las adaptaciones morfológicas del sistema osteomuscular en respuesta al ejercicio crónico. Esta revisión sistemática seleccionó artículos originales, con fecha de publicación de 2000 a 2020, idioma de publicación en inglés, con una clara intervención de ejercicio y que presentaron un cambio morfológico en el tejido estudiado. Participantes humanos independientemente de la edad, el género o condición de salud. Se identificaron 2819 registros. Después de eliminar los duplicados, la selección de títulos y resúmenes y la revisión de texto completo, se incluyeron 67 registros en el análisis final (6 para disco intervertebral, 6 para cartílago, 36 para hueso, 2 para ligamento, 9 para tendón y 7 para músculo). Los resultados destacan que las intervenciones más utilizadas fueron ejercicio aeróbico, contra resistencia y pliométrico. La población abarcó desde niños y personas sanas activas hasta personas con alguna condición de salud. Se concluye que como respuesta al ejercicio crónico existen adaptaciones morfológicas en los tejidos del sistema musculoesquelético, que pueden variar desde un aumento de rigidez hasta un aumento de área. Aunque los tejidos pueden adaptarse, aún quedan varias preguntas, como la dosis y tipo de ejercicio óptimo, si pueden ocurrir adaptaciones en un tejido lesionado y las implicaciones funcionales de estas adaptaciones. La investigación futura debe abordar estas preguntas.


León, F., Mestre. A., Priego, L. e Vera, J.C. (2023). Estudo completo de Adaptações morfológicas em resposta ao exercício crônico nos tecidos osteomusculares: uma revisão sistemática. PENSAR EN MOVIMIENTO: Revista de Ciencias del Ejercicio y la Salud, 21(1), 1-21. Até o momento, não há uma revisão sistemática que resuma as adaptações morfológicas do sistema osteomuscular em resposta ao exercício crônico. Esta revisão sistemática selecionou artigos originais, com data de publicação de 2000 a 2020, idioma de publicação em inglês, com clara intervenção de exercícios e que apresentaram alteração morfológica no tecido estudado. Participantes humanos, independentemente da idade, sexo ou condição de saúde. Foram identificados 2.819 registros. Após eliminar os artigos duplicados, triagem de título e resumo e revisão do texto completo, 67 registros foram incluídos na análise final (6 para disco intervertebral, 6 para cartilagem, 36 para osso, 2 para ligamento, 9 para tendão e 7 para músculo). Os resultados destacam que as intervenções mais utilizadas foram exercícios aeróbicos, resistidos e pliométricos. A população variou de crianças e pessoas saudáveis ativas a pessoas com alguma condição de saúde. Conclui-se que, em resposta ao exercício crônico, ocorrem adaptações morfológicas nos tecidos do sistema musculoesquelético, que podem variar desde um aumento de rigidez até um aumento de área. Embora os tecidos possam se adaptar, várias questões permanecem, como a dose ideal e o tipo de exercício, se adaptações podem ocorrer no tecido lesado e as implicações funcionais dessas adaptações. Pesquisas futuras devem abordar essas questões.


Subject(s)
Humans , Adaptation, Physiological , Exercise , Musculoskeletal System , Life Style
2.
Int. j. morphol ; 41(1): 65-72, feb. 2023. ilus
Article in English | LILACS | ID: biblio-1430530

ABSTRACT

SUMMARY: Respiration and water-liquid transportation are controlled by many factors in the lung. The aim of this study was to explore the structure and proteins expression in lungs of Phrynocephalus vlangalii by means of gross anatomy, light microscope observation, scanning electron microscope and immunohistochemistry. Results show that there were many alveoli in the lung and the walls of alveoli and capillaries were very thin. The inner surface of the lung was divided into many cystic chambers by reticular diaphragm, and the network of pulmonary capillaries was dense. Immunohistochemistry showed that AQP1 was mainly expressed in the epithelium of interstitial bronchi, parabronchiole endothelium, capillary endothelium and alveolar epithelial cells. VIP positive nerve fibers are mainly distributed in trachea, bronchial smooth muscle layer, the walls of pulmonary vessels and bronchial vessels and around submucosal glands. CECR2 is distributed in peripheral capillaries and small. Investigations of structure and proteins biology could be relevant with the adaptive strategy to drought and hypoxia environment in Phrynocephalus vlangalii.


La respiración y el transporte de agua y líquido están controlados en el pulmón por muchos factores. El objetivo de este estudio fue explorar la estructura y la expresión de proteínas en los pulmones de Phrynocephalus vlangalii por medio de la anatomía macroscópica, observación con microscopio óptico, microscopio electrónico de barrido e inmunohistoquímica. Los resultados muestran que había muchos alvéolos en el pulmón y que las paredes de los alvéolos y de los capilares eran muy delgadas. La superficie interna del pulmón estaba dividida en cámaras quísticas por el diafragma reticular y se observó una densa red de capilares pulmonares. La inmunohistoquímica mostró que AQP1 se expresaba principalmente en el epitelio de los bronquios intersticiales, el endotelio parabronquial, el endotelio capilar y las células epiteliales alveolares. Las fibras nerviosas VIP positivas se distribuyen principalmente en la tráquea, la capa de músculo liso bronquial, las paredes de los vasos pulmonares y los vasos bronquiales y alrededor de las glándulas submucosas. CECR2 se distribuye en pequeño capilares periféricos. Las investigaciones de la biología de la estructura y las proteínas podrían ser relevantes con la estrategia de adaptación al entorno de sequía e hipoxia en Phrynocephalus vlangalii.


Subject(s)
Animals , Adaptation, Physiological , Lizards/anatomy & histology , Lung/anatomy & histology , Immunohistochemistry , Microscopy, Electron, Scanning , Lung/ultrastructure
3.
Neumol. pediátr. (En línea) ; 18(2): 32-36, 2023. ilus, tab
Article in Spanish | LILACS | ID: biblio-1444098

ABSTRACT

La adaptación fisiológica de los recién nacidos de la vida intrauterina a la extrauterina incluye cambios fisiológicos complejos. Para que se complete con éxito la transición fetal a neonatal, los recién nacidos deben lograr el aclaramiento de líquido pulmonar, la generación de CRF e inicio de la respiración, para permitir el intercambio de gases y la oxigenación de los tejidos. En este artículo se describen estos mecanismos, algunas bases del desarrollo pulmonar y de la circulación fetal; y las medidas que se requieren para asistir al recién nacido cuando no logran completar esta transición espontáneamente, lo que ocurre en el 5 al 10 % de los casos.


The physiological adaptation of newborns from intrauterine to extrauterine life includes complex physiological changes. For the fetal to neonatal transition to be successfully completed, neonates must achieve lung fluid clearance, FRC generation, and initiation of respiration to allow gas exchange and tissue oxygenation. This article describes these mechanisms, some bases of lung development and fetal circulation; and the measures required to assist the newborn, when they fail to complete this transition spontaneously, which occurs in the 5 to 10% of cases.


Subject(s)
Humans , Infant, Newborn , Respiratory Physiological Phenomena , Lung/physiology , Vascular Resistance , Adaptation, Physiological , Fetus/blood supply
4.
Article in English | LILACS, BBO | ID: biblio-1432158

ABSTRACT

ABSTRACT OBJECTIVE To assess the changes in stress levels, social behavior, dietary and parafunctional habits, oral hygiene, among other conditions perceived by dental students in Brazil during the Covid-19 pandemic and evaluated the correlations between stress level and other variables. METHODS An online questionnaire was developed and validated. Undergraduates enrolled in private and public dental schools were recruited by convenience sampling. Data were collected on the perceived changes regarding stress levels, financial and social characteristics, dietary habits, oral hygiene, health conditions, and parafunctional habits. Quantitative variables were expressed as absolute and relative frequencies. Wilcoxon test evaluated comparisons between perceived changes, and correlations between changes in stress levels and other variables were analyzed by Spearman correlation (α = 0.05). RESULTS A total of 638 dental students, mean age of 22.95 ± 4.10 years, participated in the study. During the pandemic, the reported stress levels increased while household income decreased (p < 0.05). Late dinners and mindless eating increased in frequency, whereas oral hygiene decreased (p < 0.05). Most of the health conditions and parafunctional habits assessed changed (p < 0.05). Perceived stress levels showed poor negative correlations with household income (rS = −0.14), poor positive correlations with the pressure to contribute financially in the household (rS = 0.19), and poor positive correlations with food choice frequency (rS = 0.15) (p < 0.05). CONCLUSIONS Dental students reported perceived changes in stress levels, dietary habits, oral hygiene, health conditions, parafunctional habits, and social behavior. Moreover, the results showed poor correlations, as students with higher stress levels tended to have the lowest household income, feel pressured to contribute financially in the household, and present a high meal intake frequency.


Subject(s)
Humans , Male , Female , Oral Hygiene , Students, Dental , Adaptation, Physiological , Health Behavior , Physical Distancing , COVID-19
5.
Braz. j. biol ; 83: e248911, 2023. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1339362

ABSTRACT

Abstract The telencephalon refers to the most highly developed and anterior part of the forebrain, consisting mainly of the cerebral hemispheres. The study determined Neuroglobin (Ngb) and Hypoxia-inducible factor (HIF-1α) expression in the telencephalon of yak and cattle, and compare the expression and distribution pattern of Ngb and HIF-1α in the two animals. Immunohistochemistry (IHC), quantitative real-time Polymerase Chain Reaction (qRT-PCR), and Western blot (WB) were employed to investigate Ngb and Hif-1α expression in the telencephalon of yak and cattle. mRNA and protein expressions of Ngb and HIF-1α showed positive in different tissues of the yak and cattle telencephalon. Ngb expression in tissues of the yak recorded higher as compare to cattle while HIF-1α expression was found higher in cattle than yak. The HIF-1α expression in some tissues of yak telencephalon was consistent with the cattle. The results documented that HIF-1α may have a direct or indirect synergistic effect on Ngb expression in the yak telencephalon to improve hypoxia adaptation. It is suggested that yak may need more Ngb expression for adaptation, but the expression of HIF-1α seems to be down-regulated during long-term adaptation, and the specific causes of this phenomenon needs to be further verified.


Resumo O telencéfalo refere-se à parte anterior e mais desenvolvida do prosencéfalo, consistindo principalmente dos hemisférios cerebrais. O estudo determinou a expressão de neuroglobina (Ngb) e fator indutível por hipóxia (HIF-1α) no telencéfalo de iaques e bovinos e comparou a expressão e o padrão de distribuição de Ngb e HIF-1α nos dois animais. Imuno-histoquímica (IHC), reação em cadeia da polimerase quantitativa em tempo real (qRT-PCR) e Western blot (WB) foram empregados para investigar a expressão de Ngb e Hif-1α no telencéfalo de iaques e bovinos. As expressões de mRNA e proteínas de Ngb e HIF-1α mostraram-se positivas em diferentes tecidos do telencéfalo de iaque e bovino. A expressão de Ngb nos tecidos do iaque foi registrada mais alta em comparação com o gado, enquanto a expressão do HIF-1α foi encontrada mais alta no gado do que no iaque. A expressão de HIF-1α em alguns tecidos do telencéfalo de iaque foi consistente com o gado. Os resultados documentaram que o HIF-1α pode ter um efeito sinérgico direto ou indireto na expressão de Ngb no telencéfalo de iaque para melhorar a adaptação à hipóxia. É sugerido que o iaque pode precisar de mais expressão de Ngb para adaptação, mas a expressão de HIF-1α parece ser regulada para baixo durante a adaptação de longo prazo, e as causas específicas desse fenômeno precisam ser verificadas.


Subject(s)
Animals , Telencephalon , Hypoxia-Inducible Factor 1, alpha Subunit/genetics , RNA, Messenger/genetics , Cattle , Adaptation, Physiological , Neuroglobin
6.
Rev. enferm. neurol ; 21(3): 270-277, sep.-dic. 2022. tab
Article in Spanish | LILACS, BDENF | ID: biblio-1428474

ABSTRACT

Introducción: El estado funcional se mide mediante la autonomía que tienen las personas para desarrollar las actividades de la vida diaria, cuando no se pueden realizar de manera autónoma es necesario un cuidador. Esta medida se sustenta en el modelo de adaptación de Sor Callista Roy, ya que se identifica el proceso de envejecimiento como el estímulo focal que produce una respuesta inmediata en el adulto mayor de acuerdo con el modo de interdependencia. Objetivo: Conocer el nivel de dependencia funcional de los adultos mayores de la comunidad de Tula de Allende, Hidalgo. Material y métodos: Estudio de aplicabilidad básica, cuantitativo, descriptivo y trasversal. Participaron 366 adultos mayores de Tula de Allende, y se les dio a conocer los términos de confidencialidad conforme a lo establecido en la Ley General de Salud y Declaración de Helsinki mediante el consentimiento informado. Se aplicó el cuestionario de Índice de Barthel (IB), para medir la dependencia funcional en las actividades instrumentales de la vida diaria. Resultados: Predomina el género femenino con el 52.7% representado por 193 participantes, la mayoría son casadas y amas de casa. De acuerdo con el IB la mayoría de los participantes tiene una dependencia leve, con una frecuencia de 206 y un porcentaje de 56.3%, y el cruce de variables arroja como resultado una relación significativa. Conclusión: Los adultos mayores de la comunidad de Tula de Allende presentan un nivel de dependencia funcional leve.


Introduction: Functional status assesses people's ability to carry out daily activities; when these cannot be performed autonomously, a caregiver is necessary. This measure is based on the Roy Adaptation Model, which identifies the aging process as the focal stimulus that generates an effect according to the interdependence mode. Objective: To evaluate the level of functional dependence of the elderly in the community of Tula de Allende, Hidalgo. Material and methods: Quantitative, descriptive, and cross-sectional study of basic applicability. 366 older adults from Tula de Allende participated and were informed about confidentiality terms in accordance with the General Health Law and the Declaration of Helsinki through informed consent. The Barthel Index (BI) questionnaire was applied to measure functional dependence on daily instrumental activities. Results: The female gender predominates with 52.7% represented by 193 participants, the majority are married housewives. According to the BI, most of the participants have a slight dependency, with a frequency of 206 and a percentage of 56.3%; the correlation of variables showed statistical significance. Conclusion: Older adults in the community of Tula de Allende present a mild functional dependence level.


Subject(s)
Humans , Male , Female , Aged , Aged, 80 and over , Aged , Adaptation, Physiological , Functional Status
7.
Enferm. foco (Brasília) ; 13(n.esp1): 1-8, set. 2022. ilus, tab
Article in Portuguese | LILACS, BDENF | ID: biblio-1397107

ABSTRACT

Objetivo: Analisar a produção da pós-graduação brasileira relacionada à aplicação da teoria de Callista Roy. Métodos: Revisão integrativa, de cunho bibliométrico, com dados de 78 teses e dissertações brasileiras, organizados por ano e tipo de publicação; local geográfico, instituição de ensino e autoria; fomento; especialidade; produção de artigos; especialidade e foco de aplicação. Resultados: Setenta e cinco porcento das teses e dissertações estão disponíveis na íntegra; 27,0% geraram artigos; 39,7% receberam fomento, predominando bolsas; 58,9% são do Nordeste; 37,0% são direcionadas para estomaterapia e pediatria; 48,8% têm como foco o processo de enfermagem e 32,0%, a pessoa em diferentes ciclos da vida. Conclusão: A importante produção da pós-graduação brasileira sobre o tema poderia ser mais visível em artigos. As pesquisas da pós-graduação brasileira oferecem sustentação ao processo de enfermagem direcionado às necessidades de adaptação das pessoas, em distintos ciclos da vida e processo saúde-doença. (AU)


Objective: To analyze the Brazilian graduate production related to the application of Callista Roy's theory. Methods: Integrative review, biblimetric in nature, with data from 78 Brazilian theses and dissertations, organized by year and type of publication; geographic location, educational institution and authorship; promotion; specialty; production of articles; specialty and application focus. Results: Seventy-five percent of theses and dissertations are available in full; 27.0% generated articles; 39.7% received funding, predominantly scholarships; 58.9% are from the Northeast; 37.0% are directed to stomatherapy and pediatrics; 48.8% focus on the nursing process and 32.0%, the person in different life cycles. Conclusion: The important production of Brazilian graduate studies on the subject could be more visible in articles. Brazilian graduate research supports the nursing process aimed at people's adaptation needs, in different life cycles and the healthdisease process. (AU)


Objetivo: Analizar la producción de graduación brasileña relacionada con la aplicación de la teoría de Callista Roy. Métodos: Revisión integradora, de carácter biblimétrico, con datos de 78 tesis y disertaciones brasileñas, organizadas por año y tipo de publicación; ubicación geográfica, institución educativa y autoría; promoción; especialidad; producción de artículos; especialidad y enfoque de aplicación. Resultados: El setenta y cinco por ciento de las tesis y disertaciones están disponibles en su totalidad; 27,0% artículos generados; El 39,7% recibió financiación, predominantemente becas; 58,9% son del Nordeste; El 37,0% se orienta a estomaterapia y pediatría; El 48,8% se centra en el proceso de enfermería y el 32,0%, la persona en diferentes ciclos de vida. Conclusión: La importante producción de estudios de posgrado brasileños sobre el tema podría ser más visible en los artículos. La investigación de posgrado brasileña apoya el proceso de enfermería dirigido a las necesidades de adaptación de las personas, en los diferentes ciclos de vida y el proceso salud-enfermedad. (AU)


Subject(s)
Models, Nursing , Nursing Theory , Adaptation, Physiological , Adaptation, Psychological , Bibliometrics
8.
Braz. J. Vet. Res. Anim. Sci. (Online) ; 59: e181942, fev. 2022. tab, graf, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1363107

ABSTRACT

The project is based on a test of a thoracic vibration vest prototype, adapted to equines by the Expector® vest's company, on healthy animals. Ten (10) equines were used in the project, male or female, adults, healthy, belonging to FMVZ-USP or private owners. Each animal went through two phases: A and B. Phase A consisted of the placement of the vest without turning on the vibrators, evaluating the animal's acceptability, facility, adaptation to the animal's body, and discomfort due to the vest's use. Phase B included the placement of the vest and turning on the vibrators, evaluating the animal's acceptability, reaction to the vibrators, and, if present, to which velocity/type of vibration, and the presence of adverse effects. Both phases were done three times on separate days. The behavioral parameters: "placement facility" and "adaptation to the animal's body" were observed. In phase B, the response to the vibration was classified from 0 to 5. The answer was evaluated on low and high intensities for the four vibration types. The heart rate (HR) and respiratory rate (RR) were also evaluated at the beginning and end of each repetition. The animals' HR was kept on normal, except for one animal on one day of the test. Concerning the RR, most animals presented moments of tachypnea. On the experiment's first day, 100% of grade Great to "facility of placement" and "adaptation to the animal's body" was obtained, but on days 2 and 3 this value dropped to 90% due to alterations in one animal's responses. Regarding vibration's responses, 77.3% were evaluated as no discomfort (grade 0), 17.1% little discomfort (grade 1), 3.3% medium discomfort (grade 2), 0.4% great discomfort (grade 3), 0.21% extreme discomfort (grade 4), and 1.6% non-acceptance of the vest (grade 5). Some possible changes on the prototype were also verified to be suggested to the manufacturer, such as the change of the buckle and the use of wireless control. Vest use appears promising for equine respiratory physiotherapy, considering the acceptability was good, and its efficiency on the expectoration of diseased animals must be tested.


O projeto consistiu no teste de um protótipo de colete de vibração torácica, adaptado aos equinos pela empresa do colete Expector®, em animais saudáveis. Foram utilizados 10 equinos, machos ou fêmeas, adultos, saudáveis, pertencentes à FMVZ-USP e a proprietários particulares. Cada animal passou por duas fases: A e B. A Fase A consistiu na colocação do colete sem ligar os vibradores, avaliando-se a aceitabilidade do animal; facilidade; adaptação ao corpo do animal e incômodo do mesmo à sua presença. Já a Fase B contava com a colocação do colete e funcionamento dos vibradores, avaliando-se a aceitabilidade do animal; reação aos vibradores e, se presente, a qual velocidade/tipo de vibração; presença de reações ou efeitos adversos. As duas fases foram realizadas em triplicata em dias separados. Foram observados os parâmetros comportamentais "facilidade de colocação" e "adaptação ao corpo do animal". Na fase B, a resposta à vibração foi classificada de 0 a 5. A resposta foi avaliada nas intensidades baixa e alta para os quatro diferentes tipos de vibração. Foram avaliadas também as frequências cardíaca (FC) e respiratória (FR) no início e final de cada repetição. A FC dos animais se manteve dentro do intervalo de normalidade, com exceção de um animal em um dia de avaliação. Em relação à FR, a maioria apresentou momentos de taquipneia. No primeiro dia de experimento obteve-se 100% de avaliação Ótima para "facilidade de colocação" e "adaptação ao corpo do animal", mas nos dias 2 e 3 esse valor caiu para 90% devido à alteração na resposta de um animal. Em relação à resposta à vibração, 77,3% das respostas foram avaliadas como nenhum incômodo (nota 0), 17,1% pouco incômodo (nota 1), 3,3% médio incômodo (nota 2), 0,4% muito incômodo (nota 3), 0,21% incômodo extremo (nota 4) e 1,6% não aceitação do colete (nota 5). Foram também verificadas algumas possíveis mudanças no protótipo a serem sugeridas ao fabricante, como mudança do tipo de fivela e uso de controle sem fio. A utilização do colete parece ser muito promissora para a fisioterapia respiratória em equinos, visto que a aceitabilidade foi muito boa, devendo-se agora realizar a avaliação de sua eficácia na expectoração de animais enfermos.(AU)


Subject(s)
Animals , Adaptation, Physiological , Physical Therapy Modalities/veterinary , Equipment Design , Respiratory Rate/physiology , Heart Rate/physiology , Horses/physiology
9.
Bogotá; s.n; 2022. 105 p. tab, ilus.
Thesis in Spanish | LILACS, BDENF, COLNAL | ID: biblio-1399246

ABSTRACT

La presente investigación tuvo como fin medir la respuesta fisiológica (frecuencia cardiaca, saturación de oxígeno y temperatura) del niño(a) prematuro(a) o con bajo peso al nacer, con la aplicación de la técnica "Masaje al Bebé Canguro-MBC", con el animo de demostrar la seguridad de esta técnica en estos parámetros fisiológicos en el recién nacido. Por lo tanto, se efectuó un estudio cuantitativo, con diseño preexperimental de preprueba/posprueba con un solo grupo entre enero y junio del 2019 en un Programa Canguro Ambulatorio de Bogotá. Resultados: Se acogieron un total de 63 bebés canguro. Se realizó análisis estadístico con SPSS, descriptivo e inferencial con prueba de normalidad de Kolmogorov-Smirnov y métodos paramétricas t-student y no paramétricas Friedman. La frecuencia cardiaca presentó un aumento en el índice siendo la media inicial de 147.22±7.17 lpm y de 147.54±7.96 lpm después de la intervención, aunque este no fue significativo (p=0.603). Por su parte, la oximetría presentó un aumento al ser la mediana inicial de 92% y después del MBC 93% variación que fue significativa (p=0.000). Finalmente la temperatura inicial y después presentó una mediana de 36.6°C con un índice inicial de 36.59±0.169°C y de 36.62±0.163°C después de la intervención, aunque este no fue significativo (p=0.182). Se realizó un modelo de regresión ajustado a las variables fisiológicas no encontrandose factores predictores estadísticamente significativos. Desde el modelo de Callista Roy el MBC tuvo una respuesta adaptativa eficaz. Conclusiones: La técnica de MBC tiene un efecto favorable y seguro en la frecuencia cardiaca, saturación de oxigeno y temperatura sin alteración de los parámetros normales con buena adaptación según el modelo de Callista Roy.


The purpose of this research was to measure the physiological response (heart rate, oxygen saturation and temperature) of the premature or low birth weight child, with the application of the "Kangaroo Baby MassageKBM" technique. , with the aim of demonstrating the safety of this technique in these physiological parameters in the newborn. Therefore, a quantitative study, with a pre-experimental pre-test/post-test design, was carried out with a single group between January and June 2019 in a Kangaroo Outpatient Program in Bogotá. Results: A total of 63 kangaroo babies were received. Statistical analysis was performed with SPSS, descriptive and inferential with the Kolmogorov-Smirnov normality test and parametric tstudent and non-parametric Friedman methods. The heart rate presented an increase in the index, the initial mean being 147.22±7.17 bpm and 147.54±7.96 bpm after the intervention, although this was not significant (p=0.603). On the other hand, oximetry showed an increase as the initial median was 92% and after the KBM was 93%, a variation that was significant (p=0.000). Finally, the temperature presented initial and after median 36.6°C an increase with an initial index of 36.59±0.169°C and 36.62±0.163°C after the intervention, although this was not significant (p=0.182). A regression model adjusted to the physiological variables was performed, and no statistically significant predictive factors were found. From Callista Roy's model, the KBM had an effective adaptive response. Conclusions: The KBM technique has a favorable and safe effect on heart rate, oxygen saturation and temperature without alteration of normal parameters with good adaptation according to the Callista Roy model.


Subject(s)
Humans , Male , Female , Infant, Newborn , Adaptation, Physiological , Kangaroo-Mother Care Method/methods , Massage/methods , Infant, Low Birth Weight , Infant, Premature , Oxygen Saturation , Heart Rate
10.
Chinese Journal of Gastrointestinal Surgery ; (12): 1132-1137, 2022.
Article in Chinese | WPRIM | ID: wpr-971224

ABSTRACT

Intestinal adaptation is a spontaneous compensation of the remanent bowel after extensive enterectomy, which improves the absorption capacity of the remanent bowel to energy, fluid and other nutrients. Intestinal adaptation mainly occurs within 2 years after enterectomy, including morphological changes, hyperfunction and hyperphagia. Intestinal adaptation is the key factor for patients with short bowel syndrome to weaning off parenteral nutrition dependence and mainly influenced by length of remanent bowel, type of surgery and colon continuity. In addition, multiple factors including enteral feeding, glucagon-like peptide 2 (GLP-2), growth hormone, gut microbiota and its metabolites regulate intestinal adaptation via multi-biological pathways, such as proliferation and differentiation of stem cell, apoptosis, angiogenesis, nutrients transport related protein expression, gut endocrine etc. Phase III clinical trials have verified the safety and efficacy of teduglutide (long-acting GLP-2) and somatropin (recombinant human growth hormone) in improving intestinal adaptation, and both have been approved for clinical use. We aim to review the current knowledge about characteristics, mechanism, evaluation methods, key factors, clinical strategies of intestinal adaptation.


Subject(s)
Humans , Adaptation, Physiological , Glucagon-Like Peptide 2/therapeutic use , Intestines/surgery , Parenteral Nutrition , Short Bowel Syndrome/surgery
11.
Electron. j. biotechnol ; 54: 8-16, nov.2021. ilus, graf, tabs
Article in English | LILACS | ID: biblio-1510688

ABSTRACT

BACKGROUND Qaidam cattle are local breeds that habitats in northwest China. It has many excellent characteristics, such as high cold and roughage tolerance, low oxygen adaptability, and tender meat quality. Copy number variation (CNV) can induce phenotypic changes in animals by a variety of effects, and thus affects the biological functions of the animals. To explore the molecular mechanism of its adaptation to extreme cold weather and muscle fat development, the CNV variations in the genome of three Qaidam cattle were detected by whole-genome sequencing, in this study. RESULTS : A total of 16,743 CNVs and 9498 copy number variable regions (CNVRs) were obtained after the screening, which accounts for 2.18% of the bovine genome. The CNVR length detected ranged from 0.3 KB to 10.77 KB, with a total length of 58.17 MB and an average length of 6.12 KB/ CNVR. Through functional enrichment of CNVR related genes, LDHB, and ME1 genes were screened as the key genes for Qaidam cattle to adapt to the cold and low oxygen environments, whereas KIT and FGF18 genes might be related to the coat color and growth. In the CNVR overlapped with QTLs, variation in CAPN1 and CAST genes might be closely related to the tender meat quality of Qaidam cattle. CONCLUSIONS Therefore, this study provides new genetic insights on the environmental adaptability and important economic traits of Qaidam cattle


Subject(s)
Animals , Cattle , Adaptation, Physiological/genetics , Genome-Wide Association Study , Acclimatization/genetics , Cattle/genetics , China , Altitude , Genotype
12.
Rev. bras. med. esporte ; 27(4): 390-394, Aug. 2021. tab, graf
Article in English | LILACS | ID: biblio-1288600

ABSTRACT

ABSTRACT Introduction: Basketball sports will effect on the morphology and structure of the hand bones and joints. Objective: The article selected nine professional basketball players (basketball group) and 11 non-basketball players (control group) in the provincial youth team. A 64-row spiral computed tomography scan was used to scan the wrist and three-dimensional reconstruction. The volume of each carpal bone and the computed tomography value (bone density) were measured. Methods: To explore the influence of basketball sports on the hand bones and joints' morphological structure, the paper analyzes the structural characteristics of the computed tomography images of young male basketball players' wrist bones. Results: Compared with the carpal bones in the control group, the volume of the right navicular bone and the small polygonal bone, the left-hand navicular bone, the large triangular bone, and the small polygonal bone in the basketball group increased significantly (P<0.05). Conclusions: Basketball can increase the volume of the part of the wrist bones of adolescent male athletes and reduce the bone density; the morphological structure of the non-smashing wrist bones of basketball players has similar changes to that of the spikers. Level of evidence II; Therapeutic studies - investigation of treatment results.


RESUMO Introdução: O basquetebol afetará a morfologia e a estrutura dos ossos e articulações da mão. Objetivo: o artigo selecionou nove jogadores profissionais de basquete (grupo de basquete) e 11 não jogadores de basquete (grupo de controle) da equipe juvenil da província. Uma tomografia computadorizada espiral de 64 linhas foi usada para digitalizar o punho e a reconstrução tridimensional. O volume de cada osso do carpo e o valor da tomografia computadorizada (densidade óssea) foram medidos. Métodos: Para explorar a influência dos esportes de basquete sobre os ossos da mão e a estrutura morfológica das articulações, o artigo analisa as características estruturais das imagens de tomografia computadorizada de ossos do punho de jovens jogadores de basquete do sexo masculino. Resultados: Em comparação com os ossos do carpo no grupo de controle, o volume do osso navicular direito e o osso poligonal pequeno, o osso navicular esquerdo, o osso triangular grande e o osso poligonal pequeno no grupo de basquete aumentaram significativamente (P <0,05). Conclusões: O basquete pode aumentar o volume dos ossos do punho de atletas adolescentes do sexo masculino e reduzir a densidade óssea; a estrutura morfológica dos ossos do pulso que não se quebram em jogadores de basquete tem mudanças semelhantes às dos espigões. Nível de evidência II; Estudos terapêuticos- investigação dos resultados do tratamento.


RESUMEN Introducción: el baloncesto afectará la morfología y la estructura de los huesos y articulaciones de la mano. Objetivo: El artículo seleccionó nueve jugadores de baloncesto profesionales (grupo de baloncesto) y 11 jugadores no baloncesto (grupo de control) en el equipo juvenil provincial. Se utilizó una tomografía computarizada en espiral de 64 filas para escanear la muñeca y la reconstrucción tridimensional. Se midieron el volumen de cada hueso del carpo y el valor de la tomografía computarizada (densidad ósea). Métodos: Para explorar la influencia de los deportes de baloncesto en la estructura morfológica de los huesos de la mano y las articulaciones, el artículo analiza las características estructurales de las imágenes de tomografía computarizada de los huesos de la muñeca de los jóvenes jugadores de baloncesto. Resultados: En comparación con los huesos del carpo en el grupo de control, el volumen del hueso navicular derecho y el hueso poligonal pequeño, el hueso navicular izquierdo, el hueso triangular grande y el hueso poligonal pequeño en el grupo de baloncesto aumentaron significativamente (P <0,05). Conclusiones: El baloncesto puede aumentar el volumen de los huesos de la muñeca de los deportistas varones adolescentes y reducir la densidad ósea; la estructura morfológica de los huesos de las muñecas que no se rompen de los jugadores de baloncesto tiene cambios similares a los de los atacantes. Nivel de evidencia II; Estudios terapéuticos- investigación de los resultados del tratamiento.


Subject(s)
Humans , Male , Wrist Joint/diagnostic imaging , Basketball , Carpal Bones/diagnostic imaging , Bone Density/physiology , Athletes , Wrist Joint/growth & development , Algorithms , Adaptation, Physiological , Carpal Bones/growth & development , Tomography, X-Ray Computed/methods , Imaging, Three-Dimensional/methods
13.
Rev. Pesqui. Fisioter ; 11(3): 495-500, ago.2021. ilus, tab
Article in English, Portuguese | LILACS | ID: biblio-1293370

ABSTRACT

INTRODUÇÃO: A corrente interferencial é uma modalidade bastante utilizada, porém, apresenta como fator limitante a quantidade de acomodações, o que é comum em eletroestimulação. Apesar de possuir alguns recursos que visam reduzir a adaptação fisiológica, não é totalmente eficaz; neste sentido, pode haver diferenças devido à técnica de uso. Assim, o presente estudo pretendeu analisar se há diferenças na adaptação para as formas bipolar ou tetrapolar em indivíduos com dor lombar crônica não específica. MÉTODOS: Ensaio clínico cruzado, composto por 15 voluntárias com dor lombar crônica não específica, as quais receberam terapia bipolar ou tetrapolar em semanas subsequentes. Foi explicado sobre a adaptação à corrente e o que deveria avisar quando ocorresse, e, desta forma, foi computado o número de vezes em que o fenômeno ocorreu, o tempo necessário até ocorrer a primeira adaptação, a intensidade inicial da corrente utilizada e o quanto aumentou-se após a primeira adaptação. RESULTADOS: Para nenhuma das variáveis analisadas, foi observada diferença significativa entre as duas técnicas (p>0,05). CONCLUSÃO: As técnicas analisadas não mostraram diferenças na adaptação em mulheres jovens com dor lombar não específica.


INTRODUCTION: The interferential current is a widely used modality; however, it presents as a limiting factor the amount of accommodation, which is common in electrostimulation. Despite having some resources that aim to reduce physiological adaptation, it is not fully effective in this sense, but there may be differences due to the technique of use. Thus, the present study aimed to analyze whether there are differences in the accommodation for bi- or tetrapolar forms in individuals with chronic nonspecific low back pain. METHODS: Crossover clinical study, consisting of 15 volunteers with chronic nonspecific low back pain, who received bipolar or tetrapolar therapy in subsequent weeks. They were explained about the current accommodation and that they should be told when it occurred, and in this way, the number of times that the phenomenon occurred, the time needed until the first accommodation occurred, the initial intensity of the current used, and how much it increased after the first accommodation were computed. RESULTS: None of the variables analyzed had a significant difference between the two techniques (p>0.05). CONCLUSION: The techniques analyzed showed no differences in accommodation in young women with nonspecific low back pain.


Subject(s)
Humans , Female , Adolescent , Adult , Young Adult , Adaptation, Physiological , Electric Stimulation Therapy/methods , Low Back Pain/therapy , Chronic Disease , Longitudinal Studies
14.
Neotrop. ichthyol ; 19(4): e210054, 2021. tab, graf, mapas, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1351163

ABSTRACT

Gymnogeophagus labiatus and G. lacustris have been long recognized as sister species exhibiting different ecological requirements. Gymnogeophagus labiatus occurs in rock bottom rivers in the hydrographic basins of Patos Lagoon (HBP) and Tramandaí River (HBT), while G. lacustris is exclusive from sand bottom coastal lagoons of the HBT. In this study, we used molecular markers, morphological measurements and data from nuptial male coloration to investigate the evolutionary relationship between these species in each hydrographic basin. We found, for all data sets, a closer relationship between G. labiatus and G. lacustris from the HBT than between G. labiatus populations from HBT and HBP. In particular, lip area had a large intraspecific plasticity, being uninformative to diagnose G. lacustris from G. labiatus. Molecular clock-based estimates suggest a recent divergence between species in the HBT (17,000 years ago), but not between G. labiatus from HBP and HBT (3.6 millions of years ago). Finally, we also found a divergent G. labiatus genetic lineage from the Camaquã River, in the HBP. These results show that the current taxonomy of G. labiatus and G. lacustris does not properly represent evolutionary lineages in these species.(AU)


Gymnogeophagus labiatus e G. lacustris vêm sendo consideradas espécies irmãs que possuem diferentes exigências ecológicas. Gymnogeophagus labiatus ocorre em rios de fundo de pedra nas bacias hidrográficas da Laguna dos Patos (HBP) e do rio Tramandaí (HBT), enquanto G. lacustris é exclusivo da HBT, ocorrendo em lagoas costeiras de fundo de arenoso. Nesse estudo, foram usados marcadores moleculares, medidas morfológicas e dados sobre a coloração nupcial em machos para investigar a relação evolutiva entre estas espécies em cada bacia hidrográfica. Para todos os conjuntos de dados foi observada uma relação mais próxima entre G. labiatus e G. lacustris da HBT do que entre as populações de G. labiatus da HBP e HBT. Em particular, a área do lábio teve uma grande plasticidade intraespecífica, não sendo informativa para diagnosticar G. lacustris de G. labiatus. Estimativas baseadas no relógio molecular sugeriram uma divergência recente entre as espécies da HBT (17.000 anos atrás), mas não entre as populações de G. labiatus da HBP e HBT (3,6 milhões de anos atrás). Finalmente, também foi encontrada uma linhagem genética de G. labiatus divergente no rio Camaquã, na HBP. Esses resultados mostram que a taxonomia atual de G. labiatus e G. lacustris não representa adequadamente as linhagens evolutivas nessas espécies.(AU)


Subject(s)
Animals , Weights and Measures , DNA, Mitochondrial/analysis , Adaptation, Physiological , Hydrography , Cichlids
15.
Pesqui. vet. bras ; 40(12): 992-1001, Dec. 2020. tab
Article in English | LILACS, VETINDEX | ID: biblio-1155031

ABSTRACT

Studies on biochemical constituents and hydration of horses in training or competitions are essential for the knowledge of their adaptive physiological and metabolic responses to the type of exercise inherent to the sport. The present research evaluated the effects of exercise on hydric status and physiologic and biochemical profiles in Quarter Horses during a barrel race training session. The design consisted of three evaluation times (T): at rest, before the start of physical activity (T0); immediately after a 10-minute warm-up trot and then running the barrel race courses twice with a 10-minute interval (T1); and after one hour's rest after the second course (T2). The parameters analyzed were: heart rate (HR), respiratory rate (RR), body temperature (BT) and mucose color; the capillary refill time (CRT), packed cell volume (PCV), total plasma proteins (TPP), serum creatinine (CRE) and blood urea nitrogen (BUN) were evaluated and used to infer water status; plasma osmolarity (Osm) was measured; the electrolytes sodium (Na), potassium (K), chloride (Cl), total calcium (tCa), total magnesium (tMg) and phosphorus (P), plasma glucose (Glu) and lactate (Lac) and the muscle enzymes creattine kinase (CK) and aspartate aminotransferase (AST) completed the evaluated parameters. The data for the variables studied were submitted to ANOVA and the Tukey test at 5% probability. After exercise (in T1), HR, RR, BT, PCV, TPP, Glu, Lac, Cre increased (P<0.05), there was also increase in phosphorus and decrease in K (P<0.05), but there was no hypophosphatemia and hipokalemia, respectively. After rest (in T2), only glucose returned to baseline levels (T0) and, with lower mean values, HR, RR, BT, PCV, K and Lac did not return to baseline levels (T0), while P decreased (P<0.05). Osm, BUN, Na, Cl, tCa, tMg, CK and AST did not change between times (P>0.05). It was concluded that the maximum intensity exercise performed on two barrel race courses caused mild hemoconcentration, did not changed plasma osmolality or concentrations of important electrolytes and muscle enzymes of horses, but caused hyperlactatemia without the plasma lactate concentrations being fully recomposed after resting.(AU)


O perfil bioquímico e o status de hidratação de equinos em treinamentos ou competições fornecem dados importantes das suas respostas fisiológicas e metabólicas em relação ao tipo específico de exercício em uma modalidade esportiva. No presente estudo, o status hídrico e o perfil fisiológico-bioquímico de equinos foram avaliados durante treinamento de três tambores, em três tempos (T): em repouso, antes do início da atividade física (T0); imediatamente após 10 minutos de aquecimento ao trote seguido de duas corridas na pista de tambor, intervaladas em 10 minutos (T1); e após uma hora de descanso do segundo percurso (T2). Os parâmetros avaliados foram: frequência cardíaca (FC), frequência respiratória (FR), temperatura corporal (BT) e cor da mucosa; o tempo de enchimento capilar (TEC), volume globular ou hematócrito (VG), proteínas plasmáticas totais (TPP), creatinina sérica (Cre) e a ureia (Ure) foram avaliados e utilizados para inferir o estado hídrico dos animais; a osmolaridade plasmática (Osm) também foi mensurada; as concentrações dos eletrólitos sódio (Na), potássio (K), cloreto (Cl), cálcio total (tCa), magnésio total (tMg) e fósforo (P), e das enzimas musculares foram mensurados no soro, e as de glicose (Gli) e lactato (Lac) no plasma. Os dados das variáveis estudadas foram submetidos à ANOVA e ao teste de Tukey a 5% de probabilidade. Após o exercício (T1), a FC, FR, TC, VG, TPP, Gli, Lac, Cre aumentaram. O P também aumentou (P<0,05), mas não alcançou nível de hiperfosfatemia, e o K diminuiu (P<0,05), mas não ao nível de hipocalemia. Embora com médias menores, a FC, FR, TC, VG, K e o Lac não retornaram aos níveis basais (T0) após o repouso (T2), apenas a Gli, enquanto o fósforo diminuiu (P<0,05). A Osm, ureia, Na, Cl, tCa, tMg, CK e AST não variaram entre os tempos (P>0,05). Concluiu-se que, o exercício de intensidade máxima realizado em dois percursos de três tambores causou leve hemoconcentração, não alterou a osmolaridade plasmática nem importantes concentrações de eletrólitos e de enzimas musculares dos equinos, porém, causou hiperlactatemia, sem completa recomposição da concentração do lactato após uma hora de descanso.(AU)


Subject(s)
Adaptation, Physiological , Exercise Test/veterinary , Respiratory Rate , Horses/physiology , Muscles , Biochemistry
16.
Rev. peru. med. exp. salud publica ; 37(4): 733-738, oct.-dic. 2020. graf
Article in Spanish | LILACS | ID: biblio-1156809

ABSTRACT

RESUMEN Con el objetivo de describir los cambios funcionales y morfológicos tempranos en el riñón remanente de donantes vivos, se realizó un estudio retrospectivo en el Hospital Cayetano Heredia, en el que se incluyeron 55 individuos. De las historias clínicas, se obtuvieron los datos clínicos y demográficos, así como la depuración de creatinina, la proteinuria, la presión arterial y las dimensiones renales a los 1, 2, 3, 6 y 12 meses después de la donación del riñón. La edad media fue de 40,88 (±9,84) años; el 80% eran mujeres y el índice de masa corporal medio fue de 25,68 (±3,5) kg/m2. Se utilizaron modelos lineales y cuadráticos para estudiar las variables fisiológicas y morfológicas. Durante el tiempo de seguimiento, la tasa de filtración glomerular, la proteinuria, la presión arterial diastólica y la longitud de los riñones mostraron cambios significativos (p < 0,05).


ABSTRACT In order to describe the early functional and morphological changes in the remnant kidney of living donors, a retrospective study was carried out at the Cayetano Heredia Hospital. Data from 55 individuals was included. Clinical and demographic data were obtained from the clinical records, as well as data for creatinine clearance, proteinuria, blood pressure and renal dimensions at 1, 2, 3, 6 and 12 months after kidney donation. The mean age was 40.88 (±9.84) years; 80% were women and the mean body mass index was 25.68 (±3.5) kg/m2. Linear and quadratic models were used to study physiological and morphological variables. During the follow-up time, glomerular filtration rate, proteinuria, diastolic blood pressure, and kidney length showed significant changes (p < 0.05).


Subject(s)
Humans , Male , Female , Kidney Transplantation , Living Donors , Kidney , Physiology , Proteinuria , Tissue Donors , Adaptation, Physiological , Arterial Pressure , Solitary Kidney , Glomerular Filtration Rate
17.
Biosci. j. (Online) ; 36(6): 2060-2067, 01-11-2020. tab, graf
Article in English | LILACS | ID: biblio-1148068

ABSTRACT

The dual-purpose wheat stands out as a complementary alternative for forage supply during the winter period. Therefore, this work had the objective of evaluate the performance characteristics in five genotypes of dual-purpose wheat submitted to different sowing densities and different clipping managements. The experiment was carried out during the 2016 and 2017 harvests. The experimental design was a randomized block design, organized in a factorial scheme, with five dual-purpose wheat genotypes (BRS Tarumã, BRS Umbu, BRS Figueira, BRS Guatambu and BRS 277) x five sowing densities (75, 150, 225, 300 and 375 seeds m²) x four clipping operations (zero, one, two and three clippings), arranged in three replicates. The measuring of characters were number of fertile tillers, seeds yield, angle of flag leaf, chlorophyll content and flowering. The genotype BRS Tarumâ is superior in number of fertile tillers and chlorophyll index for all the sowing densities, as well, the flowering is influenced direct by clipping managements. The sowing density of 75 seeds m-2 is not indicated for dual-purpose wheat independent of the clipping management. The seeds yield is amplified thought the increase in sowing density, however, advancing in clipping levels there is a decrease in forage yield.


O trigo de duplo propósito se destaca como uma alternativa complementar para o fornecimento de forragem durante o período de inverno. Portanto, este trabalho teve como objetivo avaliar as características de desempenho de cinco genótipos de trigo de duplo propósito submetidos a diferentes densidades de semeadura e diferentes manejos de corte. O experimento foi realizado durante as safras de 2016 e 2017. O delineamento experimental foi em blocos ao acaso, organizado em esquema fatorial, com cinco genótipos de trigo de duplo propósito (BRS Tarumã, BRS Umbu, BRS Figueira, BRS Guatambu e BRS 277) x cinco densidades de semeadura (75, 150, 225, 300 e 375 m² de sementes) x quatro operações de clipagem (zero, um, dois e três recortes), dispostos em três repetições. As medidas dos caracteres foram número de perfilhos férteis, rendimento de sementes, ângulo de folha de bandeira, teor de clorofila e floração. O genótipo BRS Tarumâ é superior em número de perfilhos férteis e índice de clorofila para todas as densidades de semeadura, assim como o florescimento é influenciado diretamente pelos manejos de corte. A densidade de semeadura de 75 sementes m-2 não é indicada para trigo de dupla finalidade independente do manejo de corte. O rendimento das sementes é amplificado pelo aumento da densidade de semeadura, entretanto, avançando nos níveis de recorte, há uma diminuição no rendimento de forragem.


Subject(s)
Triticum , Adaptation, Physiological
18.
Electron. j. biotechnol ; 48: 1-12, nov. 2020. tab, graf, ilus
Article in English | LILACS | ID: biblio-1254671

ABSTRACT

BACKGROUND: The potential waste canola oil-degrading ability of the cold-adapted Antarctic bacterial strain Rhodococcus sp. AQ5-07 was evaluated. Globally, increasing waste from food industries generates serious anthropogenic environmental risks that can threaten terrestrial and aquatic organisms and communities. The removal of oils such as canola oil from the environment and wastewater using biological approaches is desirable as the thermal process of oil degradation is expensive and ineffective. RESULTS: Rhodococcus sp. AQ5-07 was found to have high canola oil-degrading ability. Physico-cultural conditions influencing its activity were studied using one-factor-at-a-time (OFAT) and statistical optimisation approaches. Considerable degradation (78.60%) of 3% oil was achieved by this bacterium when incubated with 1.0 g/L ammonium sulphate, 0.3 g/L yeast extract, pH 7.5 and 10% inoculum at 10°C over a 72-h incubation period. Optimisation of the medium conditions using response surface methodology (RSM) resulted in a 9.01% increase in oil degradation (87.61%) when supplemented with 3.5% canola oil, 1.05 g/L ammonium sulphate, 0.28g/L yeast extract, pH 7.5 and 10% inoculum at 12.5°C over the same incubation period. The bacterium was able to tolerate an oil concentration of up to 4.0%, after which decreased bacterial growth and oil degradation were observed. CONCLUSIONS: These features make this strain worthy of examination for practical bioremediation of lipid-rich contaminated sites. This is the first report of any waste catering oil degradation by bacteria originating from Antarctica.


Subject(s)
Rhodococcus/physiology , Rapeseed Oil/metabolism , Waste Products , Biodegradation, Environmental , Adaptation, Physiological , Cold Temperature , Wastewater , Hydrogen-Ion Concentration , Antarctic Regions
19.
Arch. cardiol. Méx ; 90(3): 274-283, Jul.-Sep. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1131044

ABSTRACT

Resumen Objetivo: Comprobar las diferencias morfológicas y funcionales del corazón en dos poblaciones latinoamericanas con distintas características raciales y condiciones de vida. Métodos: Mediante el ecocardiograma transtorácico se obtuvieron datos de 206 personas: 103 del poblado de Atahualpa, Ecuador (nivel del mar, edad x̄ 75 ± 4.2 años, 53 mujeres) y 103 habitantes de la Ciudad de México (altitud de 2,300 m, edad x̄ 75 ± 4.2 años, 52 mujeres). Resultados: Las diferencias significativas entre Atahualpa y la Ciudad de México fueron frecuencia cardíaca, 66 vs. 80; diámetro diastólico ventricular izquierdo, 40.8 vs. 42.7; grosor del tabique, 9.8 vs. 11.6; pared posterior, 10.2 vs. 11.8; volumen-latido en centímetros cúbicos, 53.0 vs. 46.6; volumen auricular Izquierdo, 25.8 vs. 33.6; presión sistólica de la arteria pulmonar, 27.1 vs. 42.0; gasto cardíaco, 3.1 vs. 4.8; cociente E/Ea, 6.4 vs. 9.2; área mitral, 3.4 vs. 3.0. El comparativo de la función diastólica entre Atahualpa y la Ciudad de México fue: tipo 0: 2 vs. 1; tipo 1: 96 vs. 81; tipo 2: 5 vs. 20; tipo 3: 0 vs. 1. Conclusión: Las características ecocardiográficas que identifican los cambios adaptativos del corazón en Atahualpa coinciden con personas que viven a nivel del mar y con buena actividad física y en México con los habitantes de grandes altitudes y expuestos a contaminación ambiental. La función sistólica del ventrículo izquierdo fue similar en ambas poblaciones, lo que indica que los cambios adaptativos hacen posible que el corazón sea eficaz en diferentes circunstancias del ecosistema.


Abstract Objective: To compare morphological and functional differences of the heart in two Latin American populations with different ethnicity and living conditions. Methods: Using transthoracic echocardiogram we obtained data on 206 individuals: 103 from Atahualpa, Ecuador (living at sea level, mean age: 75 ± 4.2 years, 53 women) and 103 inhabitants from Mexico City (living at 2300 m above sea level, mean age: 75 ± 4.2 years, 52 women). Results: Significant differences between Atahualpa and Mexico were: Heart rate 66 versus 80 x’, left ventricular diastolic diameter 40.8 versus 42.7, septum thickness 9.8 versus 11.6, posterior wall 10.2 versus 11.8, stroke volume cc 53.0 versus 46.6, left atrial volume 25. 8 versus 33.6, systolic pressure of the pulmonary artery 27.1 versus 42.0, cardiac output 3.1 versus 4.8, E/Ea ratio 6.4 versus 9.2, and mitral area 3.4 versus 3.0. Comparison of diastolic function between Atahualpa and Mexico was: Type 0; 2 versus 1. Type 1; 96 versus 81. Type 2; 5 versus 20 and Type 3; 0 versus 1. Conclusion: Echocardiographic characteristics that identify adaptive changes of the heart in Atahualpa are coincident with people living at sea level and with good physical activity, and Mexico City, with inhabitants living at high altitudes and exposed to environmental pollution. The systolic function of the left ventricle was similar in both populations, indicating that adaptive changes allow the heart to be effective in different circumstances of the ecosystem.


Subject(s)
Humans , Male , Female , Middle Aged , Aged , Aged, 80 and over , Adaptation, Physiological/physiology , Echocardiography , Heart/diagnostic imaging , Exercise , Cross-Sectional Studies , Ecuador , Environmental Exposure/adverse effects , Environmental Pollution/adverse effects , Altitude , Mexico
20.
Int. j. morphol ; 38(4): 1039-1046, Aug. 2020. tab, graf
Article in English | LILACS | ID: biblio-1124894

ABSTRACT

The purpose of this study was to evaluate the chronic effects of stretching exercise on soleus muscle histomorphology and histomorphometry of young and aged rats. Thirty-eight female rats were divided into young control group (YCG, n=10;274±50 g); young stretching group (YSG, n=8;274±12 g); aged control group (ACG, n=10;335±39 g); and aged stretching group (ASG, n=10;321±32g). A mechanical apparatus was used to stretch muscle in 4 repetitions, 60 s each, 30 s interval between repetitions in each session, 3 times a week for 3 weeks. Twenty-four hours after the last stretching session, soleus muscle was removed for micromorphology and immunostaining analysis. Data analyses were performed with one-way ANOVA, post-hoc Tukey, or Kruskal-Wallis tests for parametric and nonparametric, respectively (p≤0.05). Muscle fiber cross-sectional area (MFCSA) of ACG was lower (18 %) compared to the YCG. Stretching increased MFCSA comparing YSG to YCG (5,681.15± 1,943.61 µm2 vs 5,119.84±1,857.73 µm2, p=0.00), but decreased comparing ASG to ACG (3,919.54± 1,694.65 µm2 vs 4,172.82±1,446.08 µm2, p=0.00). More serial sarcomere numbers were found in the YSG than YCG (12,062.91±1,564.68 vs 10,070.39±1,072.38, p=0.03). Collagen I and collagen III were higher in YSG than ASG (7.44±7.18 % vs 0.07±0.09 %, p=0.04) and (14.37 %± 9.54 % vs 5.51 %±5.52 %, p=0.00), respectively. TNF-a was greater in ASG than YSG (43.42 %±40.19 % vs 1.72 ± 2.02 %, p=0.00). Epimysium was larger in the YSG compared to YCG (201.83±132.07 % vs 181.09±147.04 %, p=0.00). After 3-week stretching the soleus muscles from aged rats were smaller than their younger counter-parts. Interestingly, while stretching appeared to positively affect young soleus muscle, the opposite was detected in the muscle of the aged rats.


El propósito de este estudio fue evaluar los efectos crónicos del ejercicio de estiramiento sobre la histomorfología e histomorfometría del músculo sóleo de ratas jóvenes y envejecidas. Se dividieron 38 ratas hembras en un grupo control joven (YCG, n = 10; 274 ± 50 g); grupo de estiramiento joven (YSG, n = 8; 274 ± 12 g); grupo control de edad (ACG, n = 10; 335 ± 39 g); y grupo estiramiento envejecido (ASG, n = 10; 321 ± 32 g). Se usó un aparato mecánico para estirar el músculo en 4 repeticiones, 60 s cada una, intervalo de 30 s entre repeticiones en cada sesión, 3 veces por semana, durante 3 semanas. Veinticuatro horas después de la última sesión de estiramiento, se extrajo el músculo sóleo para análisis de micromorfología e inmunotinción. Los análisis de datos se realizaron con pruebas ANOVA de una vía, Tukey post-hoc o Kruskal-Wallis para pruebas paramétricas y no paramétricas, respectivamente (p≤0,05). El área de la sección transversal de fibra muscular (MFCSA) de GCE fue menor (18 %) en comparación con el GCJ. El estiramiento aumentó ASTFM comparando GEJ con GCJ (5.681,15 ± 1.943,61 µm2 vs 5.119,84 ± 1.857,73 µm2, p = 0,00), pero disminuyó comparando GEE con GCE (3.919,54 ± 1.694,65 µm2 vs 4.172,82 ± 1.446,08 µm2, p = 0,00). Se encontraron más sarcómeros en serie en el GEJ que en el GCJ (12.062,91 ± 1.564,68 vs 10.070,39 ± 1,072.38, p = 0,03). El colágeno I y el colágeno III fueron más numerosos en GEJ que en GEE (7,44 ± 7.18 % vs 0,07 ± 0,09 %, p = 0,04) y (14,37 % ± 9,54 % vs 5,51 % ± 5,52 %, p = 0,00), respectivamente. TNF-α fue mayor en GEE que GEJ (43,42 % ± 40,19 % vs 1,72 ± 2,02 %, p = 0,00). El epimisio fue mayor en el GEJ en comparación con el GCJ (201,83 ± 132,07 % vs 181,09 ± 147,04 %, p = 0,00). Después de 3 semanas de estiramiento, los músculos sóleo de las ratas envejecidas eran más pequeños que sus contrapartes más jóvenes. Curiosamente, si bien el estiramiento pareció afectar positivamente el músculo sóleo joven, se detectó lo contrario en el músculo de las ratas envejecidas.


Subject(s)
Animals , Female , Rats , Muscle, Skeletal/anatomy & histology , Muscle, Skeletal/physiology , Muscle Stretching Exercises , Adaptation, Physiological , Analysis of Variance , Rats, Wistar
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