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1.
Arch. latinoam. nutr ; 72(1): 23-30, mar. 2022. tab, graf
Article in Spanish | LILACS, LIVECS | ID: biblio-1368353

ABSTRACT

La obesidad se define por un exceso de masa grasa, sin embargo, hay otros indicadores antropométricos que pueden ser útiles para el diagnóstico de sobrepeso u obesidad; Objetivo. Determinar la exactitud diagnóstica del índice de masa corporal (IMC), circunferencia de la cintura (CC) e índice de forma corporal (ABSI) para el diagnóstico de sobrepeso u obesidad en una población adulta del Ecuador. Materiales y métodos. Se desarrolló un estudio observacional de corte transversal en el que participaron 253 sujetos con edades entre 20 y 60 años. Se midió peso, estatura, circunferencia de la cintura y porcentaje de grasa corporal (PGC). Se correlacionó el PGC con IMC, CC y ABSI y se estableció la sensibilidad y especificidad de estos indicadores para el diagnóstico de sobrepeso u obesidad con curvas ROC. Resultados. El PGC fue menor en hombres que en mujeres (30,9 vs 41,87), ABSI y CC fue mayor en hombres que en mujeres (0,079 vs 0,075) y (99,76 vs 91,25) respectivamente. Se encontró una correlación positiva fuerte (≥0,75) entre el PGC e IMC y CC. En la curva ROC, el área bajo la curva más alta se observa para el IMC (0,949), mientras que el área más baja se observa para ABSI (0,395). Conclusión. El IMC es el indicador con mayor precisión diagnóstica de sobrepeso u obesidad. ABSI no sería un indicador útil en el diagnóstico de sobrepeso u obesidad(AU)


Obesity is defined by an excess of fat mass, however, there are other anthropometric indicators that can be useful for the diagnosis of overweight or obesity; Objetive. To determine the diagnostic accuracy of the body mass index (BMI), waist circumference (WC) and body shape index (ABSI) for the diagnosis of overweight or obesity in an adult population of Ecuador. Materials and methods. An observational cross-sectional study was carried out in which 253 subjects aged between 20 and 60 years participated. Weight, height, waist circumference and percentage body fat (PBF) were measured. The PBF was correlated with BMI, WC and ABSI and the sensitivity and specificity of these indicators were established for the diagnosis of overweight or obesity with ROC curves. Results. The PBF was lower in men than in women (30.09 vs 41.87), ABSI and CC were higher in men than in women (0.079 vs 0.075) and (99.76 vs 91.25) respectively. A strong positive correlation (≥0.75) was found between % body fat and BMI and WC. On the ROC curve, the area under the highest curve is observed for BMI (0.949), while the lowest area is observed for ABSI (0.395). Conclusion. The BMI is the indicator with the highest diagnostic precision of overweight or obesity. ABSI would not be a useful indicator in the diagnosis of overweight or obesity(AU)


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Body Mass Index , Adipose Tissue , Overweight , Body Fat Distribution , Obesity , Anthropometry , Cross-Sectional Studies , Diagnosis , Ecuador , Waist Circumference
2.
Arq. ciências saúde UNIPAR ; 26(1): 33-45, Jan-Abr. 2022.
Article in Portuguese | LILACS | ID: biblio-1362669

ABSTRACT

Objetivo: Investigar a relação entre o perfil de adesão e barreiras percebidas por estudantes universitários para permanência no Programa de Extensão "Yoga: Awaken ONE". Métodos: Foram realizados dois cortes transversais com universitários da Universidade Federal do Recôncavo da Bahia. O primeiro foi composto por 16 indivíduos e investigou o perfil demográfico, socioeconômico, antropométrico, da aptidão física e da qualidade de vida dos universitários que ingressaram no Programa de Extensão. O segundo visou identificar as barreiras para permanência de 13 estudantes (dentre os 16 iniciais) que haviam se afastado do Programa após quatro meses do início. Resultados: Houve predominância de indivíduos do sexo feminino, dos cursos de graduação em Educação Física e Pedagogia e da classe socioeconômica C. A maioria dos participantes estava com indicadores adequados de gordura corporal. Observou-se grande proporção de indivíduos com indicadores baixos de flexibilidade e força muscular. Para a qualidade de vida, a menor mediana foi observada para o domínio meio ambiente e a maior para o domínio relações sociais. As principais barreiras percebidas para a prática de yoga pelos universitários foram "jornada de estudos extensa" e "jornada de trabalho extensa". Observou-se correlação do perfil sociodemográfico, indicadores de obesidade, variáveis hemodinâmicas, flexibilidade, força muscular e qualidade de vida com barreiras percebidas para permanência no Programa de Extensão universitária "Yoga: Awaken ONE". Conclusões: Estes achados sugerem que o perfil do público universitário pode ser determinante para a permanência ou evasão de programas de promoção de exercícios físicos e precisa ser considerado em propostas de programas de extensão universitária.


Objective: Investigate the relationship between the member adherence profile and barriers perceived by university students to remain in the "Yoga: Awaken ONE" Extension Program. Methods: Two cross-sections were carried out with university students from the Federal University of Recôncavo da Bahia. The first was composed of 16 individuals and investigated the demographic, socioeconomic, anthropometric, physical fitness, and quality of life profile of university students joining the Extension Program. The second aimed at identifying the barriers to remain in the program faced by 13 students (out of the initial 16) who had withdrawn from the Program four months after the beginning. Results: There was a predominance of female individuals, from undergraduate courses in Physical Education and Pedagogy, and from the C socioeconomic class. Most participants had adequate body fat indexes. There was a large proportion of individuals with low flexibility and muscle strength. For quality of life, the lowest median was observed for the environment domain, while the highest could be noted for the social relationship domain. The main barriers perceived for the practice of yoga by university students were "long study hours" and "long work hours". There was a correlation between sociodemographic profile, obesity indicators, hemodynamic variables, flexibility, muscle strength, and QOL with the perceived barriers to stay in the "Yoga: Awaken ONE" university extension program. Conclusions: These findings suggest that the profile of the university audience can be a determinant for the permanence or dropout of programs that promote physical exercise and therefore, it should be taken into consideration in proposals for university extension programs.


Subject(s)
Humans , Male , Female , Adult , Students , Universities/organization & administration , Yoga , Program Evaluation , Quality of Life/psychology , Work Hours , Exercise , Body Mass Index , Adipose Tissue , Student Health , Health Status Indicators , Range of Motion, Articular , Abdominal Fat , Adiposity , Muscle Strength , Arterial Pressure , Obesity/prevention & control
3.
Arq. bras. cardiol ; 118(3): 625-633, mar. 2022. tab, graf
Article in English, Portuguese | LILACS | ID: biblio-1364342

ABSTRACT

Resumo Fundamento O tecido adiposo epicárdico (TAE) é aumentado em comorbidades comuns na insuficiência cardíaca (IC). Dessa forma, o TAE teria o potencial de mediar efeitos que levam à deterioração da função cardíaca. Objetivos Esta metanálise tem o objetivo de investigar se a quantidade de TAE em todos os tipos de IC e cada tipo de IC são significativamente diferentes dos pacientes de controle. Métodos Esta metanálise seguiu as diretrizes da Meta-analysis of Observational Studies in Epidemiology (Metanálise de estudos observacionais em epidemiologia). A pesquisa foi realizada nos bancos de dados MEDLINE, Embase e Lilacs até novembro de 2020. Dois autores realizaram a triagem, a extração de dados e a avaliação de qualidade. Um p-valor <0,05 foi definido como estatisticamente significativo. Resultados Foram incluídos oito estudos observacionais, compreendendo 1248 pacientes no total, dos quais 574 eram de controle, 415 tinham IC com fração de ejeção reduzida (ICFER) e 259 tinham IC com fração de ejeção de faixa média ou preservada (ICFEfm ou ICFEP). A quantidade de TAE não era diferente entre todos os tipos de IC e o grupo de controle (DMP = -0,66, IC 95%: -1,54 a 0,23, p =0,14) . Analisando cada fenótipo de IC separadamente, pacientes com ICFER tinham TAE reduzido em comparação aos pacientes de controle (DMP = 1,27, IC 95%: - 1,87 a -0,67, p <0,0001), enquanto os pacientes com ICFEfm ou ICFEP tiveram TAE aumentado em comparação aos pacientes de controle (DMP = 1,24, IC 95%: 0,99 a 1,50, p <0,0001). Conclusão A quantidade de TAE não era significativamente diferente entre todos os tipos de IC e o grupo de controle. Em pacientes com ICFER o volume de TAE era reduzido, enquanto em pacientes com ICFEP e ICFEfm, a quantidade de TAE era significativamente aumentada. Número de registro PROSPERO: CRD42019134441.


Abstract Background Epicardial adipose tissue (EAT) is increased in comorbidities common in heart failure (HF). In this sense, EAT could potentially mediate effects that lead to an impaired cardiac function. Objectives This meta-analysis aims to investigate if the amount of EAT in all-types of HF and each HF phenotype is significantly different from control patients. Methods This meta-analysis followed the Meta-analysis Of Observational Studies in Epidemiology guidelines. The search was performed in the MEDLINE, Embase, and Lilacs databases until November 2020. Two authors performed screening, data extraction, and quality assessment. A p-value <0.05 was defined as statistically significant. Results Eight observational studies were included, comprehending 1,248 patients in total, from which 574 were controls, 415 had HF with reduced ejection fraction (HFrEF) and 259 had HF with mid-range or preserved ejection fraction (HFmrEF or HFpEF). The amount of EAT was not different between all types of HF and the control group (SMD = -0.66, 95% CI: -1.54 to 0.23, p =0.14). Analyzing each HF phenotype separately, patients with HFrEF had a reduced EAT when compared to the controls (SMD= -1.27, 95% CI: - 1.87 to -0.67, p <0.0001), while patients with HFmrEF or HFpEF showed an increased EAT when compared to controls (SMD= 1.24, 95% CI: 0.99 to 1.50, p <0.0001). Conclusion The amount of EAT was not significantly different between all types of HF and the control group. In patients with HFrEF, the EAT volume was reduced, whereas in HFpEF and HFmrEF, the amount of EAT was significantly increased. PROSPERO registration number: CRD42019134441.


Subject(s)
Humans , Heart Failure , Phenotype , Prognosis , Stroke Volume , Adipose Tissue , Observational Studies as Topic
4.
Braz. dent. sci ; 25(2): 1-6, 2022. ilus, tab
Article in English | LILACS, BBO | ID: biblio-1363723

ABSTRACT

Objective: The aim this research was assess the level of satisfaction of patients undergoing bichectomy and its repercussions. Material and Methods: A cross-sectional and qualitative study, under protocol number 20707519.5.0000.5207, was carried out by applying a questionnaire to patients undergoing bichectomy, which made it possible to assess complaints, degree of postoperative satisfaction, satisfaction of family members, time required to perceive results, complications and adverse effects, intensity of postoperative pain, and possibility of being submitted to a new intervention. Results: The sample consisted of 30 patients, among them, 82% sought surgery for aesthetic reasons. Regarding the degree of satisfaction, the majority (70%) were very satisfied, followed by 13.3% who were satisfied, 10% considered normal, 3.3% were dissatisfied and 3.3% very dissatisfied. Regarding the opinion of friends and family, the majority were very satisfied with the result. The adverse effects found were mild, no permanent injury happened, and the pain was mild in the most majority of the sample. Conclusion: Thus, the most majority of patients were satisfied with the results, as well as their family and friends. The results were more evident between the second and third months. No major or permanent damage was observed, suggesting that it is a safe technique when performed well.(AU)


Objetivo: Avaliar o nível de satisfação dos pacientes submetidos à bichectomia, bem como correlacionar os resultados com algumas características clínicas. Material e Métodos: Foi realizado um estudo transversal e qualitativo, sob protocolo número 20707519.5.0000.5207, através da aplicação de um questionário a pacientes submetidos à bichectomia, o qual foi possível avaliar as queixas, o grau de satisfação pós-operatório, a satisfação dos familiares, o tempo para percepção dos resultados, as complicações e efeitos adversos, intensidade da dor pós operatória, e a possibilidade de ser submetido a nova intervenção. Além disso, as características demográficas da amostra (como sexo e idade) também foi registrada na ficha de avaliação. Resultados: A amostra foi composta por 30 pacientes, e desses, 82% buscaram a cirurgia por motivos estéticos. Sobre o grau de satisfação, a maioria (70%) ficou muito satisfeitos, seguidos por 13,3% que ficaram satisfeitos, 10% consideraram normal, 3,3% insatisfeitos e 3,3% muito insatisfeitos. Sobre a opinião dos amigos e familiares, a maioria ficou muito satisfeitos com o resultado. Os efeitos adversos encontrados foram leves, nenhuma lesão permanente foi encontrada, e a dor foi discreta na grande maioria da amostra. Com base na possibilidade de submeter-se a uma nova cirurgia, 70% ficaram satisfeitos e não fariam novamente. Conclusão: A grande maioria dos pacientes demonstrou-se satisfeitos com os resultados, bem como seus familiares e amigos. Os resultados foram mais evidentes entre o segundo e terceiro mês. Não foi observado qualquer dano importante ou permanente, sugerindo ser uma técnica segura quando bem executada. (AU)


Subject(s)
Humans , Adipose Tissue , Patient Satisfaction , Esthetics, Dental , Mastication
7.
Arq. bras. cardiol ; 117(4): 737-747, Oct. 2021. tab, graf
Article in Portuguese | LILACS | ID: biblio-1345234

ABSTRACT

Resumo Fundamento A síndrome metabólica é caracterizada por um conjunto de comorbidades. Durante a síndrome, observam-se alterações estruturais no sistema cardiovascular, especialmente o remodelamento vascular. Uma das causas predisponentes para essas alterações é a inflamação crônica oriunda de mudanças na estrutura e composição do tecido adiposo perivascular. Atorvastatina é eficaz no tratamento das dislipidemias. No entanto, seus efeitos pleiotrópicos não são totalmente compreendidos. Supõe-se que, durante a síndrome metabólica, ocorre remodelamento vascular e que o tratamento com atorvastatina pode ser capaz de atenuar tal condição. Objetivos Avaliar os efeitos do tratamento com atorvastatina sobre o remodelamento vascular em modelo experimental de síndrome metabólica. Métodos Camundongos Swiss receberam dieta controle ou dieta hiperglicídica por 18 semanas. Após 14 semanas de dieta, os camundongos foram tratados com veículo ou atorvastatina (20mg/kg) durante 4 semanas. Foram avaliados o perfil nutricional e metabólico por testes bioquímicos; análise estrutural da artéria aorta por histologia e dosagem de citocinas por ensaio imunoenzimático. O nível de significância aceitável para os resultados foi p <0,05. Resultados A dieta hiperglicídica promoveu o desenvolvimento de síndrome metabólica. Tal fato culminou no remodelamento hipertrófico do músculo liso vascular e tecido adiposo perivascular. Além disso, houve aumentos das citocinas TNF-α e IL-6 circulantes e no tecido adiposo perivascular. O tratamento com atorvastatina reduziu significativamente os danos metabólicos, o remodelamento vascular e os níveis de citocinas. Conclusão Atorvastatina ameniza danos metabólicos associados à síndrome metabólica induzida por dieta hiperglicídica, além de atenuar o remodelamento vascular, sendo esses efeitos associados à redução de citocinas pró-inflamatórias.


Abstract Background Metabolic syndrome is characterized by an array of comorbidities. During this syndrome, structural changes are observed in the cardiovascular system, especially vascular remodeling. One of the predisposing causes for these changes is chronic inflammation resulting from changes in the structure and composition of perivascular adipose tissue. Atorvastatin is effective in the treatment of dyslipidemias. However, its pleiotropic effects have not been completely understood. We hypothesize that metabolic syndrome may lead to vascular remodeling and that atorvastatin therapy may be able to attenuate this condition. Objectives To assess the effects of atorvastatin therapy on vascular remodeling in an experimental model of metabolic syndrome. Methods Swiss mice received a control diet or a hyperglicemic diet for 18 weeks. After 14 weeks of diet, mice were treated with vehicle or atorvastatin (20mg/kg) during for 4 weeks. Nutritional and metabolic profiles were assessed by biochemical tests; moreover, a histological assessment of aorta structure was conducted, and cytokine levels were determined by the immunoenzyme assay. The acceptable level of significance for the results was set at p<0.05. Results Hyperglicemic diet promoted the development of metabolic syndrome. It indeed culminated in hypertrophic remodeling of vascular smooth muscle and perivascular adipose tissue. Furthermore, there were increases in the levels of circulating TNF-α and IL-6 and in the perivascular adipose tissue. Atorvastatin therapy significantly reduced metabolic damages, vascular remodeling, and cytokine levels. Conclusion Atorvastatin attenuate metabolic damages associated with metabolic syndrome induced by hyperglycemic diet, in addition to attenuating vascular remodeling; both effects are associated with reduced levels of pro-inflammatory cytokines.


Subject(s)
Animals , Mice , Metabolic Syndrome/drug therapy , Adipose Tissue , Cytokines , Vascular Remodeling , Atorvastatin/pharmacology
8.
Electron. j. biotechnol ; 52: 67-75, July. 2021. tab, graf, ilus
Article in English | LILACS | ID: biblio-1283594

ABSTRACT

BACKGROUND: Adipogenesis and fibrogenesis can be considered as a competitive process in muscle, which may affect the intramuscular fat deposition. The CCAAT/enhancer-binding protein beta (C/EBPb) plays an important role in adipogenesis, which is well-characterized in mice, but little known in bovine so far. RESULTS: In this study, real-time qPCR revealed that the level of C/EBPb was increased during the developmental stages of bovine and adipogenesis process of preadipocytes. Overexpression of C/EBPb promoted bovine fibroblast proliferation through mitotic clonal expansion (MCE), a necessary process for initiating adipogenesis, by significantly downregulating levels of p21 and p27 (p < 0.01). Also, the PPARc expression was inhibited during the MCE stage (p < 0.01). 31.28% of transfected fibroblasts adopted lipid-laden adipocyte morphology after 8 d. Real-time qPCR showed that C/EBPb activated the transcription of early stage adipogenesis markers C/EBPa and PPARc. Expression of ACCa, FASN, FABP4 and LPL was also significantly upregulated, while the expression of LEPR was weakened. CONCLUSIONS: It was concluded C/EBPb can convert bovine fibroblasts into adipocytes without hormone induction by initiating the MCE process and promoting adipogenic genes expression, which may provide new insights into the potential functions of C/EBPb in regulating intramuscular fat deposition in beef cattle.


Subject(s)
Cattle/metabolism , Adipocytes/metabolism , CCAAT-Enhancer-Binding Protein-beta/metabolism , Fibroblasts/metabolism , Adipose Tissue/metabolism , Clone Cells , Cell Proliferation , Adipogenesis , Real-Time Polymerase Chain Reaction , Mitosis , Muscles
9.
Vitae (Medellín) ; 28(2): 1-9, 2021-05-18. Ilustraciones
Article in English | LILACS, COLNAL | ID: biblio-1362620

ABSTRACT

Background: A Body Mass Index (BMI) greater than 24.9 Kg/m2 promotes chronic inflammation due to increased secretion of pro-inflammatory adipokines. Consuming fruits rich in bioactive compounds such as berries is a promising strategy to counteract this effect. Objectives: Determine the effect of osmo-dehydrated Andean Berry consumption on inflammatory biomarkers (TNF-α, IL- 6, IL-1ß, and adiponectin) and plasma antioxidant capacity in overweight and obese adults after 21 days. Methods: Andean Berry was osmo-dehydrated in 70% sucrose syrup. Antioxidant activity, proximal composition, phenolic content, microbiological analysis, and sensory analysis of the product were determined. Twenty-five obese and overweight subjects consumed 35g of osmo-dehydrated berry for 21 days. Inflammatory biomarkers and antioxidant capacity in plasma were evaluated at the beginning and end of the study. Results: Osmo-dehydrated Andean Berry presented a total phenolic content of 692.7 ± 47.4 mg Gallic Acid Equivalents/100 g. All biomarkers evaluated in the subjects showed statistically significant differences (p> 0.05), except for CRP, before and after the study. IL-6 presented the more significant reduction among all pro-inflammatory adipokines with an effect size of 18.4 Conclusions: Regular consumption of osmo-dehydrated Andean Berry contributes to decreasing pro-inflammatory biomarkers and improves the plasma antioxidant capacity of overweight and obese adults


Antecedentes: un índice de masa corporal (IMC) superior a 24.9 kg/m2 promueve la inflamación crónica debido al aumento en la secreción de adipocinas proinflamatorias. El consumo de frutas ricas en compuestos bioactivos como las bayas es una estrategia prometedora para contrarrestar este efecto. Objetivo: Determinar el efecto del consumo de agraz osmodeshidratado en biomarcadores inflamatorios (TNF-α, IL- 6, IL-1ß y adiponectina) y capacidad antioxidante plasmática de adultos con sobrepeso y obesidad después de 21 días. Métodos: El agraz fue osmo-deshidratado en jarabe de sacarosa al 70%. Se determinó la actividad antioxidante, composición proximal, contenido fenólico, análisis microbiológico y análisis sensorial del producto. Veinticinco sujetos obesos y con sobrepeso consumieron 35 g de agraz osmodeshidratado durante 21 días. Se evaluaron biomarcadores inflamatorios y capacidad antioxidante en plasma al inicio y al final del estudio. Resultados: El agraz osmodeshidratado presentó un contenido fenólico total de 692.7 ± 47.4 mg GAE / 100 g. Todos los biomarcadores evaluados en los sujetos mostraron diferencias estadísticamente significativas (p> 0.05), a excepción de la PCR, antes y después del estudio. La IL-6 presentó la mayor reducción entre todas las adipocinas proinflamatorias con un tamaño del efecto de 18.4 Conclusiones: El consumo regular de agraz osmodeshidratado contribuye a disminuir los biomarcadores proinflamatorios y mejora la capacidad antioxidante plasmática de adultos con sobrepeso y obesidad


Subject(s)
Humans , Adipose Tissue , Adipokines , Fruit , Inflammation
10.
Odontol. Clín.-Cient ; 20(1): 90-93, jan.-mar. 2021. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1368457

ABSTRACT

Oroantral communication (OAC) is characterized by formation of direct access between the oral cavity and the maxillary sinus. This access can be lined by epithelium overtime, being called oroantral fistula (OAF). Its etiology is varied; however, it is usually associated with surgical procedures in the posterior re gion of the maxilla. Diagnosis is made through clinical and imaging evaluation. The treatment involves different surgical techniques, such as use of vestibular or palatal surgical flaps, nasal septum cartilage and, more commonly, buccal adipose body. The aim of this study was to describe therapeutic approach in a case of OAF, resulted from a dental implant complication, in a 65-year-old male patient. Fistulec tomy was performed and communication was closed by advancement buccal fat pad. The patient is in complete regression of the lesion and has no recurrences... (AU)


A comunicação oro-antral (COA) é caracterizada pela formação de acesso direto entre a cavidade bucal e o seio maxilar. Com o decorrer do tempo, esse acesso passa a ser revestido por epitélio, sendo chamado de fístula oro-antral (FOA). Sua etiologia é variada, no entanto, está geralmente associada a procedimentos cirúrgicos na região posterior de maxila. O diagnóstico é realizado através do exame clínico e imaginológico. O tratamento envolve diferentes técnicas cirúrgicas, como uso de retalhos cirúrgicos vestibulares ou palatinos, cartilagem do septo nasal e, mais comumente, o corpo adiposo da bochecha. O objetivo desse trabalho foi descrever a conduta terapêutica frente a um caso de FOA, decorrente de complicação de implante dentário, em um paciente do sexo masculino de 65 anos. Foi realizada fistulectomia e fechamento da comunicação mediante avanço da bola de Bichat. O paciente encontra-se com total regressão da lesão e sem recidivas... (AU)


Subject(s)
Humans , Male , Aged , Surgical Flaps , Adipose Tissue , Oroantral Fistula , Maxillary Sinus
11.
Arq. ciências saúde UNIPAR ; 25(1): 61-77, jan-abr. 2021.
Article in Portuguese | LILACS | ID: biblio-1151421

ABSTRACT

A obesidade é caracterizada pelo aumento excessivo da gordura corporal e está ligada ao estilo de vida, ao meio ambiente e a genética do indivíduo. O equilíbrio entre ingestão e gasto energético é controlado por mecanismos neurais, hormonais, químicos e genéticos. Estudos sugerem que o gene FTO (Fat mass and obesity associated) atua como regulador primário do acúmulo de gordura corporal, quando associado a SNPs (Single Nucleotide Polymorphism) específicos, predispõe à obesidade. O propósito deste trabalho foi verificar a produção científica, analisar e catalogar os estudos de polimorfismos no gene FTO associados à obesidade e suas comorbidades. A busca por publicações entre 2009 e 2018 foi realizada na base de dados SciELO com a palavra-chave "FTO". Foram encontrados 23 artigos originais dentro dos critérios da pesquisa que correlacionam o FTO à obesidade. O nome do autor principal, país, idioma, ano de publicação, título, objetivo, polimorfismo associado e os resultados dos estudos foram extraídos e organizados para facilitar a tabulação dos dados. Também foram pesquisados os números de citações de cada artigo, utilizando-se a plataforma Google Acadêmico. Embora o Brasil se encontre em primeiro lugar em produção científica para o gene FTO na base de dados prospectada, o número de artigos originais ainda é muito modesto. Assim, os resultados encontrados podem servir de subsídio no delineamento de novas pesquisas sobre os polimorfismos do gene FTO e as causas da obesidade.


Obesity is characterized by the excessive increase in body fat and is correlated to the lifestyle, environment, and also to the genetics of the individual. The balance between energy intake and expenditure is controlled by neural, hormonal, chemical, and genetic mechanisms. Studies suggest that the FTO (fat mass and obesity associated), a gene associated with fat mass, plays a role as a primary regulator of body fat buildup, when associated to specific Single Nucleotide Polymorphisms (SNPs), causing predisposition to obesity. This paper aimed at reviewing, analyzing, and cataloguing the studies on FTO gene polymorphisms associated with obesity and its comorbidities. The search was carried out in SciELO database, checking articles published between 2009 and 2018 using the keyword "FTO". Twenty-three original articles, matching the research criteria, correlating FTO either positively or negatively with obesity, were found. The main author's name, country, language, year of publication, title, objective, associated polymorphism, and the study results were extracted and organized to facilitate data tabulation. The citation numbers for each article were also searched by using the Google Scholar platform. Although Brazil ranks first in scientific production on the FTO gene in the surveyed database, the number of original articles is still very modest. Therefore, the results found in this paper may be used as a basis for the design of new research on the FTO gene polymorphisms and the causes of obesity.


Subject(s)
Polymorphism, Single Nucleotide , Genetics , Obesity/genetics , Satiety Response , Energy Intake/genetics , Body Mass Index , Adipose Tissue , Lipid Metabolism/genetics , Nutrigenomics , Fats , Genotype , Life Style , Metabolism/genetics
12.
Biomédica (Bogotá) ; 41(1): 131-144, ene.-mar. 2021. tab, graf
Article in English | LILACS | ID: biblio-1249065

ABSTRACT

Abstract | Introduction: No equations to predict the body composition of athletes from Medellín expected to have high performance have been constructed and, thus, decisions regarding their training and nutrition plans lack support. Objective: To calculate the concurrent validity of five prediction equations for fat percentage in a group of athletes from Medellín, Colombia, expected to yield high performance. Materials and methods: We conducted a cross-sectional analysis to validate diagnostic tests using secondary-source data of athletes under the age of 18 who were part of the "Medellín Team'.' The gold standard was dual-energy X-ray densitometry (DEXA). We analyzed the Slaughter, Durnin and Rahaman, Lohman, and Johnston prediction equations, as well as the five-component model. We used the intraclass correlation coefficient to assess the consistency of the methods and the Bland-Altman plot to calculate the average bias and agreement limits of each of the equations. Results: We included 101 athletes (50,5 % of them women). The median age was 14,8 years (IR: 13,0 - 16,0). The concurrent validity was "good/excellent" for the Johnston and the Durnin and Rahaman equations and the five-components model. The Lohman equation overestimated the fat percentage in 12,7 points. All of the equations showed broad agreement limits. Conclusions: The Durnin and Rahaman and the Johnston equations, as well as the five-component model, can be used to predict the FP in the study population as they showed a "good/excellent" concurrent validity and a low average bias. The equations analyzed have low accuracy, which hinders their use to diagnose the individual fat percentage within this population.


Resumen | Introducción. La falta de ecuaciones de predicción de la composición corporal de deportistas con expectativas de alto rendimiento en Medellín dificulta la toma de decisiones para su entrenamiento y nutrición. Objetivo. Calcular la validez concurrente de cinco ecuaciones de predicción del porcentaje de grasa en un grupo de deportistas con expectativas de alto rendimiento en Medellín, Colombia. Materiales y métodos. Se hizo un estudio trasversal de validación de pruebas diagnósticas con datos de una fuente secundaria de deportistas menores de 18 años pertenecientes al "Team Medellín". La densitometría dual de rayos X (DEXA) fue la prueba de referencia. Se analizaron las ecuaciones de predicción de Slaughter, de Durnin y Rahaman, de Lohman y de Johnston, así como el modelo de cinco componentes. Para evaluar la concordancia entre los métodos se utilizó el coeficiente de correlación intraclase y se hicieron análisis de Bland y Altman para calcular el sesgo promedio y los límites de acuerdo de cada una de las ecuaciones. Resultados. Participaron 101 deportistas (50,5 % de ellos mujeres) con una mediana de edad de 14,8 años (RI: 13,0-16,0). La validez concurrente fue "buena-excelente" para las ecuaciones de Johnston, Durnin y Rahaman y el modelo de cinco componentes. La ecuación de Lohman sobreestimó el porcentaje de grasa en 12,7 puntos porcentuales, pero todas mostraron límites de acuerdo amplios. Conclusiones. En la población del estudio se pueden utilizar las ecuaciones de Durnin y Rahaman, la de Johnston y el modelo de cinco componentes para predecir el porcentaje de grasa, pues su validez concurrente fue "buena-excelente" y el sesgo promedio fue bajo. Las ecuaciones que se estudiaron tienen poca precisión, lo que dificulta utilizarlas para el diagnóstico individual del porcentaje de grasa en dicha población.


Subject(s)
Body Composition , Adipose Tissue , Absorptiometry, Photon , Nutrition Assessment , Child , Anthropometry , Nutritional Status , Adolescent
13.
Rev. cuba. invest. bioméd ; 40(1): e856, ene.-mar. 2021. tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1289444

ABSTRACT

Introducción: La identificación de variables antropométricas relacionadas con el rendimiento en un deporte representa un interés en común para los profesionales de la salud y el deporte. Objetivo: Determinar la influencia del índice de masa corporal, la masa grasa y la masa libre de grasa en la velocidad con cambios de dirección en jóvenes futbolistas. Métodos: Se aplicó un estudio descriptivo de tipo correlacional con un enfoque cuantitativo y una muestra a conveniencia conformada por 21 futbolistas. Para valorar la velocidad con cambios de dirección se utilizó el Test de sprint 20 metros con cambios de dirección y el porcentaje de grasa corporal por medio de un monitor OMRON® BF-306. Los datos fueron analizados en el paquete estadístico PSPP en el cual se aplicó la prueba de normalidad de Shapiro-Wilk y el coeficiente correlacional de Pearson (95 por ciento de confianza y un p-valor de 0,05). Resultados: Los resultados reflejaron una distribución normal (p > 0,05); sin embargo, no se obtuvieron relaciones significativas entre el índice de masa corporal (r = -0,29), masa grasa (r = -0,06) y masa libre de grasa (r = 0,06; p > 0,05) con el rendimiento en la prueba de velocidad con cambios de dirección. Conclusiones: Este estudio permitió establecer que no existe relación significativa entre el índice de masa corporal, masa grasa y masa libre de grasa con el rendimiento de la velocidad con cambios de dirección en jóvenes futbolistas(AU)


Introduction: Identification of anthropometric variables related to performance in a given sport is a common topic of interest to health and sports professionals. Objective: Determine the influence of body mass index, fat mass, and fat-free mass on change of direction speed in young soccer players. Methods: A correlational descriptive quantitative study was conducted of a convenience sample of 21 soccer players. Change of direction speed was measured with the Meters Swerve Sprint Test, whereas body fat percentage was evaluated with an OMRON® BF-306 monitor. The data were analyzed with the statistical package PSPP, with application of the Shapiro-Wilk normality test and Pearson's correlational coefficient (confidence interval 95 percent and p-value 0,05). Results: Results show a normal distribution (p > 0,05). However, a significant relationship was not found between body mass (r = -0,29), fat mass (r = -0,06) and fat-free mass (r = 0,06; p > 0,05), and performance in the swerve speed test. Conclusions: The study found that there is not a significant relationship between body mass index, fat mass and fat-free mass, and change of direction speed in young soccer players(AU)


Subject(s)
Humans , Male , Female , Soccer , Sports , Body Mass Index , Adipose Tissue , Adiposity , Reaction Time , Confidence Intervals , Anthropometry/methods , Epidemiology, Descriptive , Correlation of Data
14.
Cad. Saúde Pública (Online) ; 37(10): e00088320, 2021. tab
Article in Portuguese | LILACS | ID: biblio-1339519

ABSTRACT

Resumo: O objetivo do estudo foi verificar os fatores associados à elevada adiposidade corporal em adolescentes. Trata-se de um estudo transversal tendo como fonte de dados uma coorte de nascimentos iniciada em São Luís, Maranhão, Brasil, em 1997/1998 e reentrevistada em 2016. A adiposidade corporal foi mensurada por meio de pletismografia por deslocamento de ar. Variáveis sociodemográficas, hábitos de vida e alimentares foram incluídos como possíveis fatores associados à elevada adiposidade corporal, sendo estratificada por sexo. A adiposidade corporal foi considerada elevada quando ≥ 25% para o sexo masculino e ≥ 30% para o sexo feminino. Verificaram-se maiores prevalências de alta adiposidade corporal nas adolescentes do sexo feminino que tinham 19 anos (RP = 1,17; IC95%: 1,02-1,35), que consumiam bebida alcoólica (RP = 1,14; IC95%: 1,00-1,30), as que nunca faziam o desjejum (RP = 1,46; IC95%: 1,17-1,81) ou o almoço (RP = 1,51; IC95%: 1,18-1,93). Os adolescentes do sexo masculino que tinham maior prevalência de elevada adiposidade corporal eram ativos fisicamente (RP = 1,49; IC95%: 1,07-2,09). Menor prevalência de elevada adiposidade corporal foi encontrada nos adolescentes do sexo masculino pertencentes à classe econômica D/E (RP = 0,38; IC95%: 0,16-0,90). Conclui-se que adolescentes do sexo feminino de 19 anos, que consumiam bebida alcoólica, e as que não costumavam fazer o desjejum e o almoço tinham maior adiposidade corporal, assim como os adolescentes do sexo masculino ativos fisicamente. Enquanto adolescentes do sexo masculino pertencentes à classe socioeconômica D/E tinham menor adiposidade corporal.


Abstract: The study aimed to verify factors associated with increased body fat in adolescents. This was a cross-sectional study in which the data source was a birth cohort launched in São Luís, Maranhão State, Brazil, in 1997/1998 and revisited in 2016. Body fat was measured with air displacement plethysmography. Sociodemographic, lifestyle, and dietary variables were included as possible factors associated with elevated body fat, stratified by sex. Body fat was considered elevated when ≥ 25% for males and ≥ 30% for females. The highest prevalence rates of high body fat were associated with female gender and age 19 years (PR = 1.17; 95%CI: 1.02-1.35), alcohol consumption (PR = 1.14; 95%CI: 1.00-1.30), and never eating breakfast (PR = 1.46; 95%CI: 1.17-1.81) or lunch (PR = 1.51; 95%CI: 1.18-1.93). Male adolescents with the highest prevalence of high body fat were physically active (PR = 1.49; 95%CI: 1.07-2.09). Lower prevalence of high body fat was found in male adolescents belonging to economic class D/E (PR = 0.38; 95%CI: 0.16-0.90). In conclusion, female adolescents 19 years of age that consumed alcohol and that did not eat breakfast or lunch had higher body fat, as did physically active male adolescents. Meanwhile, male adolescents in socioeconomic class D/E had less body fat.


Resumo: El objetivo del estudio fue verificar los factores asociados a la elevada adiposidad corporal en adolescentes. Se trata de un estudio transversal, teniendo como fuente de datos una cohorte de nacimientos iniciada en São Luís, Maranhão, Brasil, en 1997/1998 y reentrevistada en 2016. La adiposidad corporal se midió mediante pletismografía por desplazamiento de aire. Las variables sociodemográficas, hábitos de vida y alimentarios fueron incluidos como posibles factores asociados a la elevada adiposidad corporal, siendo estratificada por sexo. La adiposidad corporal se consideró elevada cuando ≥ 25% para el sexo masculino y ≥ 30% para el sexo femenino. Se verificaron mayores prevalencias de alta adiposidad corporal en las adolescentes del sexo femenino que tenían 19 años (RP = 1,17; IC95%: 1,02-1,35), que consumían bebida alcohólica (RP = 1,14; IC95%: 1,00-1,30), quienes nunca tomaban el desayuno (RP = 1,46; IC95%: 1,17-1,81) o la comida (RP = 1,51; IC95%: 1,18-1,93). Los adolescentes del sexo masculino que tenían una mayor prevalencia de elevada adiposidad corporal eran activos físicamente (RP = 1,49; IC95%: 1,07-2,09). Menor prevalencia de elevada adiposidad corporal se encontró en los adolescentes del sexo masculino, pertenecientes a la clase económica D/E (RP = 0,38; IC95%: 0,16-0,90). Se concluye que las adolescentes del sexo femenino de 19 años, que consumían bebida alcohólica y las que no estaban acostumbradas a desayunar y comer tenían mayor adiposidad corporal, así como los adolescentes del sexo masculino activos físicamente. Mientras que los adolescentes del sexo masculino pertenecientes a la clase socioeconómica D/E tenían menor adiposidad corporal.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Young Adult , Adipose Tissue , Life Style , Socioeconomic Factors , Brazil/epidemiology , Prevalence , Cross-Sectional Studies
15.
Cad. Saúde Pública (Online) ; 37(2): e00061619, 2021. tab, graf
Article in English | LILACS | ID: biblio-1153688

ABSTRACT

Intrauterine life is a critical period for the development of body fat and metabolic risk. This study investigated associations between birth weight and total and truncal body fat in adults. To do so, we analyzed data on 10,011 adults participating in the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) who self-reported birth weight as < 2.5kg, 2.5-4.0kg, or > 4.0kg at baseline (2008-2010) and underwent bioimpedance in the next follow-up visit (2012-2014). Greater mean total and truncal fat mass were seen in those with high birth weight compared with adequate birth weight (p < 0.001) in both sexes (total fat: 25.2 vs. 23.1kg in men and 31.4 vs. 27.7kg in women, and truncal fat: 13.5 vs. 12.4kg in men and 15.9 vs. 14.2kg in women). U-shaped patterns were observed in restricted cubic-spline analyses in the subset of 5,212 individuals reporting exact birth weights, although statistically significant only for those with high birth weight. In the whole sample, in comparing high to adequate birth weight, the latter predicted having a large (> 85 percentile) total and truncal fat mass, respectively: OR = 1.76, 95%CI: 1.37-2.25 (men) and OR = 1.86, 95%CI: 1.42-2.44 (women); OR = 1.68, 95% CI: 1.31-2.16 (men) and OR = 1.73, 95%CI: 1.31-2.28 (women). However, low birth weight predicted having a large (> 85 percentile) % truncal fat only in women (OR = 1.40, 95%CI: 1.03-1.91). In conclusion, in these men and women born in a period in which fetal malnutrition was prevalent, birth weight showed complex, frequently non-linear associations with adult body fat, highlighting the need for interventions to prevent low and high birth weight during pregnancy.


A vida intrauterina é um período crítico para o desenvolvimento da gordura corporal e risco metabólico. O estudo investigou as associações entre peso ao nascer e gordura corporal total e de tronco em adultos. Analisamos os dados de 10.011 participantes do Estudo Longitudinal de Saúde no Adulto (ELSA-Brasil), com peso ao nascer de < 2,5kg, 2,5-4,0kg ou > 4,0kg, autorrelatado na linha de base (2008-2010) e que fizeram exame de bioimpedância na visita seguinte (2012-2014). A gordura corporal total e de tronco mais elevada estava associada com peso ao nascer elevado, quando comparado ao peso adequado (p < 0,001) em ambos os sexos (gordura total: 25,2 vs. 23,1 kg em homens e 31,4 vs. 27,7kg em mulheres, e gordura de tronco: 13,5 vs. 12,4kg em homens e 15,9 vs. 14,2kg em mulheres). Foram observados padrões em "U" nas análises de splines cúbicos restritos, no subconjunto de 5.212 indivíduos que informaram o peso ao nascer com exatidão, embora com significância estatística apenas para aqueles com peso ao nascer alto. Na amostra total, o peso ao nascer alto (comparado com o adequado) predizia (> percentil 85) gordura corporal total e de tronco, respectivamente: OR = 1,76, IC95%: 1,37-2,25 (homens) e OR = 1,86, IC95%: 1,42-2,44 (mulheres); OR = 1,68, IC95%: 1,31-2,16 (homens) e OR = 1,73, IC95%: 1,31-2,28 (mulheres). Entretanto, baixo peso ao nascer predizia gordura de tronco elevada (> percentil 85) apenas nas mulheres (OR = 1,40, IC95%: 1,03-1,91). O estudo conclui que nesse grupo de homens e mulheres que nasceram numa época em que a desnutrição fetal era prevalente, o peso ao nascer mostrou associações complexas, frequentemente não lineares, com a gordura corporal na idade adulta, o que enfatiza a necessidade de intervenções para prevenir, durante a gestação, o baixo e alto peso ao nascer.


La vida intrauterina es un periodo crítico para el desarrollo de la masa de grasa corporal y riesgo metabólico. Investigamos las asociaciones entre peso al nacer y la grasa total y troncal en adultos. Analizamos datos de 10.011 adultos que participaron en el Estudio Longitudinal de Salud en Adultos (ELSA-Brasil) quienes autoinformaron de un peso al nacer < 2,5kg, 2,5-4,0kg, o > 4,0kg en la base de referencia (2008-2010) y experimentaron bioimpedancia en la siguiente visita de seguimiento (2012-2014). La mayor media de masa grasa total y troncal se observó en quienes tuvieron un alto peso al nacer, en comparación con quienes tuvieron un adecuado peso al nacer (p < 0,001) en ambos sexos (grasa total: 25,2 vs. 23,1kg en hombres y 31,4 vs. 27,7kg en mujeres, y grasa troncal: 13,5 vs. 12,4kg en hombres y 15,9 vs. 14,2kg en mujeres). Se observaron patrones en forma de "U" en análisis spline cúbicos restringidos en el subconjunto de 5.212 personas que informaron de sus pesos exactos al nacer, pese a que eran estadísticamente significativos solamente quienes tenían un alto peso al nacer. En toda la muestra con alto peso al nacer, comparada con el adecuado peso al nacer, se pronosticó contar con más masa grasa total y troncal (> percentil 85), respectivamente: OR = 1,76, IC95%: 1,37-2,25 (hombres) y OR = 1,86, IC95%: 1,42-2,44 (mujeres); OR = 1,68, IC95%: 1,31-2,16 (hombres) y OR = 1,73, IC95%: 1,31-2,28 (mujeres). No obstante, contar con un bajo peso al nacer predispuso a contar con más masa grasa troncal solamente en mujeres (> percentil 85) % (OR = 1,40, IC95%: 1,03-1,91). En conclusión, en estos hombres y mujeres nacidos durante un período en el que la malnutrición fetal era prevalente, el peso al nacer mostró frecuentemente asociaciones no lineales complejas, con grasa corporal en la etapa adulta, resaltando la necesidad de intervenciones para prevenir el bajo y el alto peso al nacer durante el embarazo.


Subject(s)
Humans , Male , Female , Pregnancy , Adult , Adipose Tissue , Obesity , Birth Weight , Body Composition , Body Weight , Brazil/epidemiology , Body Mass Index , Longitudinal Studies
16.
ABCD arq. bras. cir. dig ; 34(3): e1591, 2021. tab, graf
Article in English, Portuguese | LILACS | ID: biblio-1355519

ABSTRACT

ABSTRACT Background: Abdominal obesity or android obesity, that is, the increase in adipose tissue in the abdominal region, is considered a risk factor for several morbidities. Different ways of quantifying it have been proposed, one method is the measurement of the abdominal fat area by computed tomography. Aim: To establish correspondence between the groups defined by degree of obesity in relation to the total, subcutaneous and visceral fat area. Methods: Cross-sectional observational study carried out through the analysis of tomographic examinations. Horos v3.3.5 medical image visualization software was used, with abdominal tomography in a single cut including the L4 vertebral body and the umbilical scar, to obtain the areas of total, visceral and subcutaneous fat. Results: Of the 40 patients, 10 had grade II obesity, 23 grade III and 7 superobese. The amount of total fat showed an increase in relation to the degree of obesity. Visceral fat did not show significant differences between the degrees of obesity, but the data showed a lower average in the group of obesity grade II. The area of subcutaneous fat, as well as total fat, showed an increase in its measurements, according to the progression of the patients' BMI, but there was no statistical significance in this difference between the groups of grade II and super-obese individuals. Conclusion: The area of total and subcutaneous fat showed an increase in its measurements according to the progression of the BMI groups, which did not happen with visceral fat.


RESUMO Racional: A obesidade abdominal ou a obesidade androide, isto é, o aumento de tecido adiposo na região abdominal, é considerada fator de risco para diversas morbidades. Diferentes formas de quantificá-la foram propostas, sendo um dos métodos a medida da área da gordura abdominal pela tomografia computadorizada. Objetivo: Estabelecer correspondência entre os grupos definidos por grau de obesidade em relação a área de gordura total, subcutânea e visceral. Métodos: Estudo observacional analítico transversal realizado através da análise de exames tomográficos. Utilizou-se software de visualização de imagens médicas Horos v3.3.5, com tomografia abdominal em um único corte incluindo corpo vertebral de L4 e a cicatriz umbilical, para obter as áreas de gordura total, visceral e subcutânea. Resultados: Dos 40 pacientes 10 apresentavam obesidade grau II, 23 grau III e 7 superobesos. A quantidade de gordura total demonstrou aumento com relação ao grau de obesidade. A gordura visceral não apresentou diferenças significativas entre os graus de obesidade, porém os dados demonstraram média menor no grupo de obesidade grau II. A área de gordura subcutânea, assim como a gordura total, apresentou aumento de suas medidas, conforme a progressão do IMC dos pacientes, porém não houve significância estatística nesta diferença entre os grupos de obesos grau II e superobesos. Conclusão: A área de gordura total e subcutânea apresentaram aumento de suas medidas conforme a progressão dos grupos de IMC, o que não aconteceu com a gordura visceral.


Subject(s)
Humans , Adipose Tissue , Obesity , Tomography, X-Ray Computed , Body Mass Index , Cross-Sectional Studies , Intra-Abdominal Fat/diagnostic imaging
17.
Braz. j. med. biol. res ; 54(2): e9944, 2021. tab, graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS | ID: biblio-1142581

ABSTRACT

The aim of this study was to inhibit adipogenic differentiation by transfecting two growth factors, platelet-derived growth factor (PDGF-BB) and bone morphogenic protein 2 (BMP-2), into modified rat bone marrow mesenchymal stem cells (rBMSCs) and then compounded with platelet-rich plasma (PRP). To achieve rBMSCs, the osteoporosis model of rats was established, and then the rBMSCs from the rats were isolated and identified. Co-transfection of rBMSCs with PDGF-BB-GFP and BMP-2 and detection of PDGF-BB/BMP-2 expression in transfected BMSCs was assessed by qRT-PCR and western blot, respectively. Moreover, the effect of the two growth factors transfection of rBMSCs on adipogenic differentiation was evaluated by oil red O staining and western blot, respectively. Finally, construction of the two growth factors transfection of rBMSCs compounded with PRP and detection of adipogenic differentiation were assessed by oil red O staining, CCK-8, and western blot, respectively. In vitro studies revealed that the two growth factors transfection of rBMSCs compounded with PRP promoted cell viability and inhibited adipogenic differentiation and could be promising for inhibiting adipogenic differentiation.


Subject(s)
Animals , Rats , Cell Differentiation , Adipose Tissue/cytology , Platelet-Rich Plasma , Bone Morphogenetic Protein 2/genetics , Mesenchymal Stem Cells/cytology , Becaplermin/genetics , Transfection , Cells, Cultured
18.
National Journal of Andrology ; (12): 882-885, 2021.
Article in Chinese | WPRIM | ID: wpr-922170

ABSTRACT

Objective@#To investigate the application of suprapubic lipectomy with a "Ω" incision to removal of the prepubic fat pad for the management of buried penis in obese adult patients.@*METHODS@#We retrospectively analyzed the clinical data on 20 obese adult patients with buried penis treated by suprapubic lipectomy via a "Ω" incision between August 2016 and September 2019.@*RESULTS@#The operations were successfully completed in all the cases, with a mean operation time of 3.7 ± 0.6 hours and an average hospital stay of 8.3 ± 3.3 days. There were no such severe surgery-related complications as hematoma, urethral injury, or fat embolism in any of the cases. Fat liquefaction-related superficial wound infection developed in 1 patient postoperatively, which was cured by combined topical and systemic antibiotic therapy. A 3-month follow-up showed a 95% satisfaction of the patients with the postoperative appearance of the penis and suprapubic incision, but no complications such as ED, abnormal penile sensation, or penile retraction.@*CONCLUSIONS@#Suprapubic lipectomy with a "Ω" incision to remove the prepubic fat pad is an effective surgical approach to the management of buried penis in obese adult males, which is an anatomy-based surgical correction and has the advantages of slight injury, rapid recovery and few complications./.


Subject(s)
Adipose Tissue , Humans , Lipectomy , Male , Obesity/surgery , Penis/surgery , Retrospective Studies
19.
Chinese Medical Journal ; (24): 2730-2737, 2021.
Article in English | WPRIM | ID: wpr-921205

ABSTRACT

BACKGROUND@#Shenque (CV8) acupoint is located on the navel and has been therapeutically used for more than 2000 years in Traditional Chinese Medicine (TCM). However, clinical research on the underlying therapeutic molecular mechanisms of the CV8 acupoint lags far behind. This study aimed to study the mechanisms of umbilical acupoint therapy by using stem cells.@*METHODS@#The morphological characteristics of CV8 acupoint were detected under a stereomicroscope using hematoxylin and eosin (H&E) staining. Oil Red, Masson, and immunohistochemical staining on multi-layered slices were used to identify the type of cells at the CV8 acupoint. Cell proliferation was measured by a cell counting kit-8 (CCK-8) method. Flow cytometry and immunohistochemistry were used for cell identification. Induced differentiation was used to compare the differentiation of cells derived from CV8 acupoint and non-acupoint somatic stem cells into other cell types, such as osteogenic, adipogenic, and neural stem cell-like cells.@*RESULTS@#Morphological observations showed that adipose tissues at the linea alba of the CV8 acupoint in mice had a mass-like distribution. Immunohistochemical staining confirmed the distribution of stem cell antigen-1 (Sca-1) positive cells in the multi-layered slices of CV8 acupoint tissues. Cells isolated from adipose tissues at the CV8 acupoint exhibited high expression of Sca-1 and CD44 and low expression of CD31 and CD34, and these cells possessed osteogenic, adipogenic, and neurogenic stem cell-like cell differentiation ability. The cell proliferation (day 4: 0.5138 ± 0.0111 vs. 0.4107 ± 0.0180, t = 8.447, P = 0.0011; day 5: 0.6890 ± 0.0070 vs. 0.5520 ± 0.0118, t = 17.310, P 100 μm: 2.6000 ± 0.5477 vs. 0.8000 ± 0.8367, t = 4.025, P = 0.0038) were significantly enhanced in somatic stem cells derived from the CV8 acupoint compared to somatic stem cells from the groin non-acupoint. However, cells possessed significantly weaker osteogenicity ([2.697 ± 0.627]% vs. [7.254 ± 0.958]%, t = 6.893, P = 0.0023) in the CV8 acupoint group.@*CONCLUSIONS@#Our study showed that CV8 acupoint was rich with adipose tissues that contained abundant somatic stem cells. The biological examination of somatic stem cells derived from the CV8 acupoint provided novel insights for future research on the mechanisms of umbilical therapy.


Subject(s)
Acupuncture Points , Adipose Tissue , Adult Stem Cells , Animals , Cell Differentiation , Cells, Cultured , Mice , Osteogenesis
20.
Article in Chinese | WPRIM | ID: wpr-878987

ABSTRACT

To investigate the effect of Gegen Qinlian Decoction(GQD) on enzyme activity, gene expression and methylation level of fatty acid synthase(FASN) in adipose tissue from rats with insulin resistance induced by high-fat diet. The 60% fat-powered high-fat diet was continuously given to male SD rats to induce the insulin resistance model. Then, they were divided into five groups randomly and administrated by gavage every day for 16 weeks with following drugs respectively: 10 mL·kg~(-1)water for control group(C) and insulin resistance model control group(IR), 1.65 g·kg~(-1)GQD per day for low-dose group(GQDL), 4.95 g·kg~(-1)GQD per day for medium-dose group(GQDM), 14.85 g·kg~(-1)GQD per day for high-dose group(GQDH), and 5 mg·kg~(-1) rosiglitazone per day for rosiglitazone group(RGN). Epididymal adipose tissue was taken to determine enzyme activity of FASN by colorimetric method, mRNA expression level of Fasn by quantitative Real-time PCR(Q-PCR) and CpGs methylation level between +313 and +582 by bisulfite sequencing PCR(BSP). These results showed that Fasn expression was significantly lowered in IR model rats compared with the control rats(P<0.01). Enzymatic activity and CpGs methylation level of Fasn in IR group showed downward trends. Low and medium-dose GQD can increase enzyme activity of FASN(P<0.05). Moreover, low-dose GQD increased the total CpGs methylation level of Fasn fragment between +313 and +582 in insulin resistance rats(P<0.05). For GQDM group, the methylation frequency of CpGs at positions +506 and +508(P<0.01) as well as the methylation frequency of CpGs on the binding sites of transcription factorzinc finger protein 161(P<0.05) were significantly increased. The methylation frequency of CpG at +442 position was positively correlated with Fasn expression(P<0.01, r=0.735), and methylation frequencies of CpGs at +345 and +366 positions were positively associated to enzyme activity of FASN respectively(P<0.05, r=0.479; P<0.01, r=0.640). In conclusion, GQD can reverse enzyme activity of FASN and methylation level of Fasn in adipose tissue of insulin resistant rats, and CpG sites at positions +506 and +508 may be the targets of GQD. The methylation level of CpGs at + 345 and + 366 sites were possibly related to FASN activity, while methylation of CpG at + 442 site may be closely correlated with mRNA level of Fasn. In addition, GQD did not significantly change mRNA expression level of Fasn, but effectively reversed enzymatic activity, suggesting that GQD may regulate the post transcriptional expression of Fasn.


Subject(s)
Adipose Tissue , Animals , Drugs, Chinese Herbal , Fatty Acid Synthases/genetics , Gene Expression , Insulin Resistance/genetics , Male , Methylation , Rats , Rats, Sprague-Dawley
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