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1.
Chinese Journal of Biotechnology ; (12): 961-977, 2023.
Article in Chinese | WPRIM | ID: wpr-970416

ABSTRACT

Aromatic compounds are a class of organic compounds with benzene ring(s). Aromatic compounds are hardly decomposed due to its stable structure and can be accumulated in the food cycle, posing a great threat to the ecological environment and human health. Bacteria have a strong catabolic ability to degrade various refractory organic contaminants (e.g., polycyclic aromatic hydrocarbons, PAHs). The adsorption and transportation are prerequisites for the catabolism of aromatic compounds by bacteria. While remarkable progress has been made in understanding the metabolism of aromatic compounds in bacterial degraders, the systems responsible for the uptake and transport of aromatic compounds are poorly understood. Here we summarize the effect of cell-surface hydrophobicity, biofilm formation, and bacterial chemotaxis on the bacterial adsorption of aromatic compounds. Besides, the effects of outer membrane transport systems (such as FadL family, TonB-dependent receptors, and OmpW family), and inner membrane transport systems (such as major facilitator superfamily (MFS) transporter and ATP-binding cassette (ABC) transporter) involved in the membrane transport of these compounds are summarized. Moreover, the mechanism of transmembrane transport is also discussed. This review may serve as a reference for the prevention and remediation of aromatic pollutants.


Subject(s)
Humans , Adsorption , Bacteria/metabolism , Organic Chemicals , Biological Transport , ATP-Binding Cassette Transporters , Polycyclic Aromatic Hydrocarbons/metabolism
2.
Rev. odontol. UNESP (Online) ; 52: e20230021, 2023. ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1530303

ABSTRACT

Introdução: A importância da rugosidade para a osseointegração se dá pela correlação das interações das proteínas com a superfície do implante, a adsorção. Diferentes métodos são utilizados nos tratamentos de superfície, em que ocorre a remoção de partes da camada superficial, como o ataque ácido. Objetivo: Analisar as alterações de rugosidade na superfície dos implantes osseointegrados tratadas por duplo ataque ácido com uso único e até quatro reutilizações dos ácidos. Material e método As amostras de implantes dentários de liga de titânio (SINGULAR Implants ®) (n = 10) foram subdividas em cinco grupos: controle, tratamento 1, 2, 3 e 4, conforme as reutilizações dos ácidos. As capturas dos implantes no aumento de 500x, 1000x, 2000x e 4000x foram realizadas no microscópio eletrônico de varredura, para posterior análise de rugosidade pelo software ImageJ. Os valores de Ra e Rq foram analisados estatisticamente pelo teste ANOVA e o pós-teste de Tukey. Resultado: Os dados obtidos pelas imagens capturadas e pelos números de Ra e Rq adquiridos pelo ImageJ demonstraram que o tratamento com o duplo ataque ácido com cinco utilizações dos mesmos ácidos foi positivo para o tratamento de superfície do implante de liga de titânio. Conclusão: Pode-se concluir que até quatro reutilizações dos ácidos não interferiu estatisticamente nos valores de rugosidade em diferentes aumentos. São necessários mais estudos referentes à reutilização dos ácidos, contribuindo para a diminuição dos custos aos fabricantes, aumentando a sustentabilidade e mantendo-se a qualidade do produto.


Introduction: The importance of roughness for osseointegration is due to the configuration of proteins with the surface of the implant, and adsorption. Different methods are used in surface treatments, in which parts of the surface layer are removed, such as acid attack. Objective: To analyze changes in roughness on the surface of osseointegrated implants treated by double acid etching with single-use and up to 4 reuses of serums at a magnification of 500x, 1000x, 2000x, and 4000x in the scanning microscope (SEM). Method Samples of adherent implants (SINGULAR Implants ®) of lever alloy (n=10) were subdivided into 5 groups: control, treatment 1, 2, 3, and 4, according to the reuses. For the surface topography of the samples, SEM was used, followed by analysis by ImageJ software. Using the ANOVA test and Turkeys post-test, Ra and Rq values were statistically analyzed. Result: The data obtained both by the images captured in the SEM and by the Ra and Rq numbers acquired by the ImageJ illustrate that the treatment with the double acid attack with 5 uses of the same ones served, was positive for the surface treatment of the alloy implant of independent. Conclusion: It can be concluded that up to four reuses of food do not statistically interfere with roughness values. More studies are needed regarding the reuse of acids, certainly to reduce costs to manufacturers, increasing sustainability while maintaining product quality.


Subject(s)
Surface Properties , Titanium , Dental Implants , Analysis of Variance , Osseointegration , Adsorption , Microscopy, Electron, Scanning
3.
Cambios rev med ; 21(2): 837, 30 Diciembre 2022. ilus, tabs.
Article in Spanish | LILACS | ID: biblio-1416043

ABSTRACT

INTRODUCCIÓN. La falla hepática ya sea aguda o crónica reagudizada representa un reto para el clínico ya que sus complicaciones conllevan una gran mortalidad, esto se ve aún más complicado ya que las opciones terapéuticas son limitadas, incluso muchas veces no se puede acceder a un programa de trasplante hepático oportuno que mejore la sobrevida de estos pacientes, es así que se ha desarrollado un sistema de "diálisis" hepática conocido como sistema de recirculación de adsorbentes moleculares el cual hace un efecto de detoxificación para eliminar sustancias que generan una noxa en el cuerpo humano. OBJETIVO. Entender la utilidad del sistema recirculante molecular adsorbente en la falla hepática, conocer sus indicaciones y complicaciones. METODOLOGÍA. Se realizó una revisión de la literatura con un enfoque descriptivo, retrospectivo cualitativo no experimental, de documentos que tratan sobre la utilización del sistema MARS para tratar la falla hepática, con evidencia desde el año 2004 hasta el 2021. La revisión bibliográfica se llevó a cabo en bases de datos como Pubmed, Embase, BVS, Google Scholar y Elsevier. RESULTADOS. Se identificaron 30 artículos que cumplieron criterios de inclusión de un grupo original de 343 artículos revisados. Se ha determinado que la evidencia sobre este sistema está compuesta sobre todo por reportes de caso y son pocos los ensayos controlados aleatorizados que empleen su uso, sin embargo, se ha podido determinar que este sistema es un puente al trasplante renal mientras se estabiliza al paciente en la Unidad de Cuidados Intensivos, disminuye los marcadores de falla hepática. CONCLUSIÓN. En Latinoamérica su uso es casi nulo de ahí la necesidad de entender el mecanismo de este novedoso sistema.


INTRODUCTION. Hepatic failure, whether acute or chronic, represents a challenge for the clinician since its complications entail a great mortality, this is even more complicated since the therapeutic options are limited, even many times it is not possible to access a timely liver transplant program to improve the survival of these patients, Thus, a hepatic "dialysis" system known as molecular adsorbent recirculation system has been developed, which has a detoxification effect to eliminate substances that generate a noxa in the human body. OBJECTIVE. To understand the usefulness of the molecular adsorbent recirculating system in liver failure, to know its indications and complications. METHODOLOGY. A literature review was performed with a descriptive, retrospective qualitative non-experimental qualitative approach, of papers dealing with the use of the MARS system to treat liver failure, with evidence from 2004 to 2021. The literature review was conducted in databases such as Pubmed, Embase, BVS, Google Scholar and Elsevier. RESULTS. Thirty articles were identified that met inclusion criteria from an original group of 343 articles reviewed. It has been determined that the evidence on this system is mainly composed of case reports and there are few randomized controlled trials that employ its use, however, it has been determined that this system is a bridge to renal transplantation while the patient is stabilized in the Intensive Care Unit, decreasing the markers of liver failure. CONCLUSIONS. In Latin America its use is almost null, hence the need to understand the mechanism of this novel system.


Subject(s)
Humans , Male , Female , Hemodialysis Solutions/chemistry , Hepatic Encephalopathy , Liver Failure/therapy , Adsorption , Albumins/therapeutic use , Intensive Care Units , Liver Failure, Acute , Liver Failure , Dialysis , Albumins , Ecuador , Liver Diseases
4.
Arq. Ciênc. Vet. Zool. UNIPAR (Online) ; 25(2): e6378, jul-dez. 2022.
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1399609

ABSTRACT

Os resíduos provenientes da aquicultura são derivados da ração e da excreção dos peixes e podem estar sedimentados, suspensos ou dissolvidos, ocasionando elevados valores de DBO, DQO, nitrogênio e fósforo. A produção de camarões no Brasil tem gerado elevadas quantidades de resíduos sólidos, tendo em vista que os exoesqueletos dos camarões correspondem a cerca de 40% do seu peso total, resultando num forte impacto ambiental. Diversas pesquisas envolvendo a quitina estão sendo desenvolvidas na área de tratamento de água, devido principalmente a sua capacidade de formar filme, sendo utilizada em sistemas filtrantes. Este polissacarídeo também pode ser utilizado como agente floculante no tratamento de efluentes, como adsorvente na clarificação de óleos, e principalmente na produção de quitosana. Atualmente a quitosana possui aplicações multidimensionais, desde áreas como a nutrição humana, biotecnologia, ciência dos materiais, indústria farmacêutica, agricultura, terapia genética e proteção ambiental. A quitosana é muito eficiente na remoção de poluentes em diferentes concentrações. Apresenta alta capacidade e grande velocidade de adsorção, boa eficiência e seletividade tanto em soluções que possuem altas ou baixas concentrações. O uso da biotecnologia, através do processo de adsorção utilizando adsorventes naturais e baratos, como a quitina e quitosana, minimiza os impactos ambientais da aquicultura tanto em relação aos provocados pelo lançamento de efluentes no meio ambiente quanto aos causados pelo descarte inadequado dos resíduos do processamento de camarões.(AU)


Aquaculture residues are derived from fish feed and excretion and may be sedimented, suspended or dissolved, resulting in high BOD, COD, nitrogen and phosphorus values. Shrimp production in Brazil has generated high amounts of solid waste, since shrimp exoskeletons account for about 40% of their total weight, resulting in a strong environmental impact. Several researches involving chitin are being developed in the area of water treatment, mainly due to its ability to form film, being used in filter systems. This polysaccharide can also be used as a flocculating agent in the treatment of effluents, as an adsorbent in the clarification of oils, and especially in the production of chitosan. Currently, chitosan has multidimensional applications, from areas such as human nutrition, biotechnology, materials science, pharmaceutical industry, agriculture, gene therapy and environmental protection. Chitosan is very efficient in the removal of pollutants at different concentrations. It presents high capacity and high adsorption velocity, good efficiency and selectivity both in solutions that have high or low concentrations. The use of biotechnology, through the adsorption process using natural and cheap adsorbents such as chitin and chitosan, minimizes the environmental impacts of aquaculture both in relation to those caused by the release of effluents into the environment and those caused by the inappropriate disposal of processing residues of shrimps.(AU)


Los residuos procedentes de la acuicultura se derivan de la ración y de la excreción de los peces y pueden estar sedimentados, suspendidos o disueltos, ocasionando elevados valores de DBO, DQO, nitrógeno y fósforo. La producción de camarones en Brasil ha generado grandes cantidades de residuos sólidos, teniendo en cuenta que los exoesqueletos de los camarones corresponden a cerca del 40% de su peso total, resultando en un fuerte impacto ambiental. Varias investigaciones involucrando la quitina se están desarrollando en el área de tratamiento de agua, debido principalmente a su capacidad de formar película, siendo utilizada en sistemas filtrantes. Este polisacárido también puede ser utilizado como agente floculante en el tratamiento de efluentes, como adsorbente en la clarificación de aceites, y principalmente en la producción de quitosana. Actualmente la quitosana posee aplicaciones multidimensionales, desde áreas como la nutrición humana, biotecnología, ciencia de los materiales, industria farmacéutica, agricultura, terapia genética y protección ambiental. La quitosana es muy eficiente en la eliminación de contaminantes en diferentes concentraciones. Presenta alta capacidad y gran velocidad de adsorción, buena eficiencia y selectividad tanto en soluciones que poseen altas o bajas concentraciones. El uso de la biotecnología, a través del proceso de adsorción utilizando adsorbentes naturales y baratos, como la quitina y quitosana, minimiza los impactos ambientales de la acuicultura tanto en relación a los provocados por el lanzamiento de efluentes en el medio ambiente en cuanto a los causados por el descarte inadecuado de los residuos del procesamiento de camarones.(AU)


Subject(s)
Chitin/administration & dosage , Adsorption/drug effects , Chitosan/administration & dosage , Wastewater/chemistry , Biopolymers/analysis , Aquaculture , Eutrophication/physiology , Ammonia/chemistry
5.
Acta bioquím. clín. latinoam ; 56(2): 181-186, abr. 2022. graf
Article in Spanish | LILACS, BINACIS | ID: biblio-1402955

ABSTRACT

Resumen La enfermedad de Chagas es una parasitosis producida por Trypanosoma cruzi, prevalente principalmente en el continente americano, y observada en regiones no endémicas, producto de viajes y migraciones. El objetivo de este estudio fue comparar el desempeño del ensayo Elecsys® Chagas (Roche Diagnostics Alemania) (ECLIA) para el diagnóstico de la infección chagásica crónica con el método estándar y evaluar su posible empleo en reemplazo del método automatizado existente. Se estudiaron 77 muestras de sueros pertenecientes a pacientes con diagnóstico presuntivo de enfermedad de Chagas, procesadas por los distintos métodos disponibles en la Sección Parasitología del Hospital Muñiz: inmunoensayo quimioluminiscente de micropartículas (CMIA) (Abbott), enzimoinmunoanálisis de adsorción (ELISA) (Wiener) y hemaglutinación indirecta (HAI) (Lab. Lemos S.R.L.). Los resultados de los métodos ELISA y HAI fueron comparados con los obtenidos en la prueba ECLIA, y estos a su vez con el método automatizado disponible. De las muestras analizadas, 22 (28,57%) presentaron IgG anti-T. cruzi y 55 (71,43%) resultaron negativas. Con el método ECLIA se logró un 100% en los parámetros de desempeño, con diferencias en los intervalos de confianza. La razón de verosimilitud positiva y la razón de verosimilitud negativa clasificaron al ensayo como excelente y la potencia global del test apoyó esa afirmación. Los métodos inmunológicos automatizados ayudan a la performance diagnóstica en la etapa crónica de la enfermedad de Chagas, permiten minimizar errores, favorecen la velocidad de emisión de los resultados y, debido a su alta sensibilidad y especificidad, en ciertos escenarios podrían proponerse para usar como única técnica.


Abstract Chagas disease is a parasitosis caused by Trypanosoma cruzi, prevalent mainly in the American continent, and observed in non-endemic regions as a result of travel and migration. The objective of this study was to compare the performance of the Elecsys® Chagas (Roche Diagnostics Alemania) (ECLIA) assay for the diagnosis of chronic Chagas infection with the diagnostic standard, and to evaluate its possible use as a replacement for the existing automated method. A total of 77 serum samples belonging to patients with a presumptive diagnosis of Chagas disease were evaluated, processed by the different methods available in the Parasitology Section of Hospital Muñiz: microparticle chemiluminescent immunoassay (CMIA) (Abbott), enzyme-linked immunosorbent assay (ELISA) (Wiener) and indirect hemagglutination (HAI) (Lab. Lemos S.R.L). The results of the ELISA and HAI methods were compared with those obtained in the ECLIA test, and these in turn with the available automated method. Of the samples analysed, 22 (28.57%) presented IgG anti-T. cruzi and 55 (71.43%) were negative. With the ECLIA method, 100% was achieved in the performance parameters, with differences in the confidence intervals. The positive likelihood ratio and the negative likelihood ratio classify the essay as excellent, and the overall power of the test supports this statement. Automated immunological methods help diagnostic performance in the chronic stage of Chagas disease, allow minimising errors, favour the speed of issuance of results, and due to the high sensitivity and specificity, in certain scenarios, they could be proposed for use as single technique.


Resumo A doença de Chagas é uma parasitose causada pelo Trypanosoma cruzi, prevalente principalmente no continente americano, e observada em regiões não endêmicas em decorrência de viagens e migrações. O objetivo deste estudo foi comparar o desempenho do ensaio Elecsys® Chagas (Roche Diagnostics Alemanha) (ECLIA) para o diagnóstico da infecção crônica de Chagas com o método padrão e avaliar seu possível uso em substituição do método automatizado existente. Foram avaliadas 77 amostras de soro pertencentes a pacientes com diagnóstico presuntivo de doença de Chagas, processadas pelos diferentes métodos disponíveis na Seção de Parasitologia do Hospital Muñiz: imunoensaio quimioluminescente de micropartículas (CMIA) (Abbott), ensaio imunoenzimático de adsorção (ELISA) (Wiener) e hemaglutinação indireta (HAI) (Lab. Lemos S.R.L). Os resultados dos métodos ELISA e HAI foram comparados com os obtidos no teste ECLIA, e estes por sua vez com o método automatizado disponível. Das amostras analisadas, 22 (28,57%) apresentaram IgG anti-T. cruzi e 55 (71,43%) foram negativos. Com o método ECLIA, foram obtidos 100% nos parâmetros de desempenho, com diferenças nos intervalos de confiança. A razão de verossimilhança positiva e a razão de verossimilhança negativa classificam o ensaio como excelente, e a potencia geral do teste conformou essa afirmação. Os métodos imunológicos automatizados auxiliam no desempenho diagnóstico na fase crônica da doença de Chagas, permitem minimizar erros, favorecem a rapidez na emissão dos resultados e, devido à alta sensibilidade e especificidade, em determinados cenários, poderiam ser propostos para uso como técnica única.


Subject(s)
Humans , Trypanosoma cruzi , Immunoenzyme Techniques , Chagas Disease , Infections , Parasitic Diseases , Parasitology , Trypanosoma cruzi/growth & development , Trypanosoma cruzi/parasitology , Immunoglobulin G , Enzyme-Linked Immunosorbent Assay , Immunoassay , Potency , Sensitivity and Specificity , Chagas Disease/prevention & control , Adsorption , Serum , Diagnosis , Efficiency , Belonging , Hemagglutination , Methods
6.
Braz. j. biol ; 82: e244496, 2022. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1278474

ABSTRACT

Enzymes immobilized onto substrates with excellent selectivity and activity show a high stability and can withstand extreme experimental conditions, and their performance has been shown to be retained after repeated uses. Applications of immobilized enzymes in various fields benefit from their unique characteristics. Common methods, including adsorption, encapsulation, covalent attachment and crosslinking, and other emerging approaches (e.g., MOFs) of enzyme immobilization have been developed mostly in recent years. In accordance with these immobilization methods, the present review elaborates the application of magnetic separable nanoparticles and functionalized SBA-15 and MCM-41 mesoporous materials used in the immobilization of enzymes.


Enzimas imobilizadas em substratos com excelente seletividade e atividade apresentam alta estabilidade e podem suportar condições experimentais extremas, e seu desempenho foi mantido após repetidos usos. As aplicações de enzimas imobilizadas em vários campos se beneficiam de suas características únicas. Métodos comuns, incluindo adsorção, encapsulamento, ligação covalente e reticulação, e outras abordagens emergentes (por exemplo, MOFs) de imobilização de enzima, foram desenvolvidos principalmente nos últimos anos. De acordo com esses métodos de imobilização, a presente revisão elabora a aplicação de nanopartículas magnéticas separáveis e materiais mesoporosos funcionalizados SBA-15 e MCM-41 usados na imobilização de enzimas.


Subject(s)
Enzymes, Immobilized/metabolism , Magnetite Nanoparticles , Enzyme Stability , Adsorption , Hydrogen-Ion Concentration
7.
China Journal of Chinese Materia Medica ; (24): 2923-2930, 2021.
Article in Chinese | WPRIM | ID: wpr-888030

ABSTRACT

The present study determined the quantitative markers of total proanthocyanidins in the purification of the industrial waste Choerospondias axillaris pericarp based on the comparison results of high-performance liquid chromatography(HPLC) and mass spectrometry(MS) and optimized the purification process with two stable procyanidins as markers. The adsorption and desorption of five different macroporous adsorption resins, the static adsorption kinetics curve of NKA-Ⅱ resin, the maximum sample load, and the gradient elution were investigated. The UPLC-Q-TOF-MS/MS was employed for qualitative analysis of the newly-prepared total proanthocyanidins of C. axillaris pericarp. As revealed by the results, NKA-Ⅱ resin displayed strong adsorption and desorption toward total proanthocyanidins. The sample solution(50 mg·mL~(-1)) was prepared from 70% ethanol crude extract of C. axillaris pericarp dissolved in water and 7-fold BV of the sample solution was loaded, followed by static adsorption for 12 h. After 8-fold BV of distilled water and 6-fold BV of 10% ethanol were employed to remove impurities, the solution was eluted with 8-fold BV of 50% ethanol, concentrated, and dried under reduced pressure, and purified total proanthocyanidin powder was therefore obtained. Measured by vanillin-hydrochloric acid method, the purity and transfer rate of total proanthocyanidins were 47.67% and 59.92%, respectively, indicating the feasibi-lity of the optimized process. UPLC-Q-TOF-MS/MS qualitative analysis identified 16 procyanidins in C. axillaris total proanthocyanidins. The optimized purification process is simple in operation and accurate in component identification, and it can be applied to the process investigation of a class of components that are difficult to be separated and purified. It can also provide technical support and research ideas for the comprehensive utilization of industrial waste.


Subject(s)
Adsorption , Anacardiaceae , Chromatography, High Pressure Liquid , Plant Extracts , Proanthocyanidins/analysis , Resins, Synthetic , Tandem Mass Spectrometry
8.
Chinese Journal of Medical Instrumentation ; (6): 492-496, 2021.
Article in Chinese | WPRIM | ID: wpr-922045

ABSTRACT

Atherosclerosis is a chronic inflammatory disease commonly seen in clinical practice. It can lead to thickening of vascular intima, occlusion of lumen stenosis and thrombosis, leading to angina pectoris, hypertension, myocardial infarction and other diseases, posing a serious threat to human life and health. This study provides a method for removing shield needles from graphene oxide thrombus and its preparation. The graphene oxide shield needle mainly includes flexible rotating shaft, radial flexible rod, rotating needle, adsorption main pipe and dosing main pipe, laser measuring device, high definition camera and other structures, which has the following advantages:firstly, it achieves multi-angle rotation grinding thrombosis, precise rotation grinding, avoids vascular damage and infection; secondly, thrombolytic drugs can be applied in the process of rotary grinding and small thrombus can be adsorbed to effectively avoid secondary embolization of blood vessels; thirdly, it a coating of graphene oxide on a rotating needle, which protects against bacteria and infection. This study has practical reference value for the development of thrombotherapy and the application of graphene in the medical field.


Subject(s)
Humans , Adsorption , Graphite , Needles , Thrombosis/prevention & control
9.
Journal of Biomedical Engineering ; (6): 1087-1096, 2021.
Article in Chinese | WPRIM | ID: wpr-921849

ABSTRACT

Fibrinogen (Fg) in human plasma plays an important role in hemostasis, vascular repair and tissue integrity. The surface chemistry of extracellular matrix or biological materials affects the orientation and distribution of Fg, and changes the exposure of integrin binding sites, thereby affecting its adhesion function to platelets. Here, the quantity, morphology and side chain exposure of Fg adsorbed on hydrophilic, hydrophobic and avidin surfaces were measured by atomic force microscopy (AFM) and flow cytometry (FCM), then the rolling behavior of platelets on Fg was observed through a parallel plate flow chamber system. Our results show that the hydrophobic surface leads to a large amount of cross-linking and aggregation of Fg, while the hydrophilic surface reduces the adsorption and accumulation of Fg while causing the exposure and spreading of the α chain on Fg and further mediating the adhesion of platelets. Fg immobilized by avidin / biotin on hydrophilic surface can maintain the monomer state, avoid over exposure and stretching of α chain, and bind to the platelets activated by the A1 domain of von Willebrand factor instead of inactivated platelets. This study would be helpful for improving the blood compatibility of implant biomaterials and reasonable experimental design of coagulation


Subject(s)
Humans , Adsorption , Blood Platelets , Fibrinogen , Platelet Adhesiveness , von Willebrand Factor
10.
Chinese Journal of Contemporary Pediatrics ; (12): 180-185, 2021.
Article in Chinese | WPRIM | ID: wpr-879831

ABSTRACT

OBJECTIVE@#To study the efficacy and safety of double plasma molecular absorption system (DPMAS) in the treatment of pediatric acute liver failure (PALF).@*METHODS@#A prospective analysis was performed on the medical data of children with PALF who were hospitalized in the Intensive Care Unit (ICU), Hunan Children's Hospital, from March 2018 to June 2020. The children were randomly divided into two groups:plasma exchange group (PE group) and DPMAS group (@*RESULTS@#Compared with the PE group, the DPMAS group had a significantly lower number of times of artificial liver support therapy and a significantly shorter duration of ICU stay (@*CONCLUSIONS@#DPMAS is safe and effective in the treatment of PALF and can thus be used as an alternative to artificial liver support therapy.


Subject(s)
Child , Humans , Adsorption , Liver Failure, Acute/therapy , Plasma , Plasma Exchange , Prospective Studies
11.
Chinese Journal of Biotechnology ; (12): 4075-4082, 2021.
Article in Chinese | WPRIM | ID: wpr-921488

ABSTRACT

The rapid screening of tumor markers is a challenging task for early diagnosis of cancer. This study aims to use highly sensitive chemiluminescent protein microarray technology to efficiently screen a variety of low abundance tumor related markers. A new material, termed integrated polydimethylsiloxane modified silica gel (iPDMS), was obtained by adding a surface polymerization initiator with olefin end to the conventional polydimethylsiloxane, and fixing into the three-dimensional structure of polydimethylsiloxane by thermal crosslinking through silicon hydrogen bonding. In order to make the iPDMS material resistant to non-specific protein adsorption, a poly(OEGMA) polymer brush was synthesized by surface-initiated atom transfer radical polymerization at the active initiation site. Finally, 20 tumor-related antigens were printed into the specific areas of the microarray by high-throughput spray printing technology, and assembled into 48-well detection microtiterplates of the iPDMS microarray. It was found the VEGFR and VEGF121 autoantibodies that obtained from 8 common tumors (breast cancer, lung cancer, colon cancer, gastric cancer, liver cancer, leukemia, lymphoma and ovarian cancer) can be used as potential tumor markers. The chemiluminescence labeled iPDMS protein microarray can be used for the screening of tumor autoantibodies at early stage.


Subject(s)
Adsorption , Autoantibodies , Dimethylpolysiloxanes , Protein Array Analysis , Silica Gel , Surface Properties
12.
Electron. j. biotechnol ; 46: 22-29, jul. 2020. tab, graf
Article in English | LILACS | ID: biblio-1223226

ABSTRACT

BACKGROUND: Removal of dyes from wastewater by microorganisms through adsorption, degradation, or accumulation has been investigated. Biological methods used for dye treatment are generally always effective and environmentally friendly. In this study, biosorption of the Fast Black K salt azo dye by the bacterium Rhodopseudomonas palustris 51ATA was studied spectrophotometrically, at various pH (2­10), temperatures (25°C, 35°C, and 45°C) and dye concentrations (25­400 mg L-1). RESULTS: The bacterial strain showed extremely good dye-removing potential at various dye concentrations. IR studies at different temperatures showed that the dye was adsorbed on the bacterial surface at lower temperatures. Characteristics of the adsorption process were investigated by Scatchard analysis at 25°C and 35°C. Scatchard analysis of the equilibrium binding data for the dye on this bacterium gave rise to linear plots, indicating that the Langmuir model could be applied. The regression coefficients obtained for the dye from the Freundlich and Langmuir models were significant and divergence from the Scatchard plot was observed. CONCLUSION: The adsorption behavior of the dye on this bacterium was expressed by the Langmuir, Freundlich, and Temkin isotherms. The adsorption data with respect to various temperatures provided an excellent fit to the Freundlich isotherm. However, when the Langmuir and Temkin isotherm models were applied to these data, a good fit was only obtained for the dye at lower temperatures, thus indicating that the biosorption ability of R. palustris 51ATA is dependent on temperature, pH, and dye concentration.


Subject(s)
Rhodopseudomonas/metabolism , Diazonium Compounds/metabolism , Coloring Agents/metabolism , Temperature , Azo Compounds/analysis , Azo Compounds/metabolism , Contaminant Removal , Adsorption , Coloring Agents/analysis , Wastewater , Hydrogen-Ion Concentration
13.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 862-870, May-June, 2020. ilus, tab
Article in English | LILACS, VETINDEX | ID: biblio-1129541

ABSTRACT

The aim of this study was to evaluate in vitro the probiotic potential and absorption of Saccharomyces cerevisiae for the aflatoxin B1 in simulated fish intestinal tract conditions. Three yeast strains were used, two from brewery: S. cerevisiae RC1 and S. cerevisiae RC3 and one from a fish farming environment: S. cerevisiae A8L2. The selected yeasts were subjected to the following in vitro tests: homologous inhibition, self-aggregation, co-aggregation, antibacterial activity, gastrointestinal conditions tolerance and adsorption of AFB1. All S. cerevisiae strains showed good capability of self-aggregation and co-aggregation with pathogenic bacteria. All yeast strains were able to survive the gastrointestinal conditions. In acidic conditions, the factors (strain vs. time) had interaction (P=0.0317), resulting in significant variation among the strains tested in the time periods analyzed. It was observed that there was also interaction (P=0.0062) in intestinal conditions, with an increased number of cells in the 12-hour period for all strains tested. In the adsorption test, the A8L2 strain was statistically more effective (P<0.005) for both AFB1 concentrations evaluated in this study (10 and 25ng/mL). Thus, it was observed that the strains of S. cerevisiae have potential probiotic and adsorbent of AFB1.(AU)


Objetivou-se, com esta pesquisa, avaliar in vitro o potencial probiótico e adsorvente de Saccharomyces cerevisiae para aflatoxina B1 em condições simuladas do trato intestinal de peixes. Foram utilizadas três cepas de leveduras, sendo duas provenientes de cervejaria: S. cerevisiae RC1 e S. cerevisiae RC3, e uma de ambiente de piscicultura: S. cerevisiae A8L2. As leveduras selecionadas foram submetidas aos seguintes testes in vitro: inibição homóloga, autoagregação, coagregação, atividade antibacteriana, viabilidade às condições gastrointestinais e adsorção de AFB1. Todas as estirpes de S. cerevisiae mostraram boa capacidade de autoagregação e coagregação com bactérias patogênicas. Todas as estirpes de levedura foram capazes de sobreviver às condições gastrointestinais. Em condições ácidas, os fatores (cepa x tempo) tiveram interação (P=0,0317), resultando em variações significativas entre as cepas testadas nos períodos de tempo analisados. Observou-se que também houve interação (P=0,0062) em condições intestinais, havendo um aumento do número de células no período de 12h para todas as cepas avaliadas. No ensaio de adsorção, a estirpe A8L2 foi a mais eficaz estatisticamente (P<0,005), para as duas concentrações de AFB1 avaliadas neste estudo (10 e 25ng. mL-1). Dessa forma, conclui-se que as cepas de Saccharomyces cerevisiae possuem potencial probiótico e adsorvente de AFB1.(AU)


Subject(s)
Animals , Saccharomyces cerevisiae , Aflatoxin B1/antagonists & inhibitors , Probiotics/therapeutic use , Fishes/physiology , Intestines/microbiology , In Vitro Techniques , Adsorption
15.
Article in English | LILACS, BBO | ID: biblio-1135520

ABSTRACT

Abstract Objective: To determine diametral tensile strength and water absorption ability of Carbonate-apatite blocks fabricated from gypsum precursors (CaSO4) through a dissolution-precipitation method, with different solution molarities and immersion times. Material and Methods: Thirty-six CaSO4 gypsum specimens with 6 mm diameter; 3 mm height (Group A for diametral tensile strength) and 36 specimens of 6 mm diameter; 3 mm thickness (Group B for water absorption ability) were used. Each group was divided into 4 group treatments of: 1) dissolution-precipitation in solution of 0.5mol / L Na2CO3 + 0.5mol / L Na3PO4 for 48 hours and 72 hours; 1mol / L Na2CO3 + 1mol / L Na3PO4 for 48 hours and 72 hours. The C-Ap blocks were then tested using ATR-FTIR spectrometer to identify the formation of C-Ap functional groups. Furthermore, Group A specimens were tested for diametral tensile strength using Universal Testing Machine, and Group B specimens were tested for water adsorption ability using an analytical balance by measuring initial and final weight after immersion in saline solution at 37oC for 24 hours. Results: The formation of carbonate-apatite (C-Ap) in groups with solution molarity of 1 mol/L for 48 hours and 72 hours resulted in a lower diametral tensile strength and water absorption ability than the groups with a 0.5 mol/L solution. Conclusion: Solution with different molarities and dissolution-precipitation duration affect the formation of carbonate-apatite blocks.


Subject(s)
Tensile Strength , Calcium Sulfate/therapeutic use , Adsorption , Dental Materials , Analysis of Variance , Spectroscopy, Fourier Transform Infrared/instrumentation , Statistics, Nonparametric , Indonesia/epidemiology
16.
Braz. arch. biol. technol ; 63: e20180614, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132182

ABSTRACT

Abstract The organic compound caffeine when detected in environmental matrices such as surface waters and groundwater is considered as an emerging contaminant, in which its effects are still unknown. Therefore, in the present research, zinc oxide-based catalysts impregnated with iron and silver were prepared for the reaction of caffeine degradation by heterogeneous photocatalysis. The wet impregnation method with excess solvent was applied to the preparation of the materials, later they were characterized by adsorption of N2, X-ray diffraction and photoacoustic spectroscopy. Then, the photodegradation, photolysis and adsorption tests were carried out, in which it was observed that only the presence of the radiation or photocatalysts could not sufficiently degrade the caffeine, however when combined radiation with all the catalysts studied here presented degradation above 70% at the end of 300 minutes of the reaction, and the best catalyst studied was that containing 8% Ag in non-calcined ZnO. Thus, these results point out that the methodology employed in this research, both for the preparation of the catalysts and in the process of the photocatalysis reaction, was efficient in the degradation of the emerging contaminant, caffeine, which could later be used for a mixture of other contaminants.


Subject(s)
Silver/chemistry , Zinc Oxide/chemistry , Caffeine/chemistry , Catalysis , Photochemical Processes , Adsorption , Bioreactors , Iron/chemistry
17.
Braz. arch. biol. technol ; 63: e20180637, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132189

ABSTRACT

Abstract This study describes the use of bentonite in suspension for the caffeine adsorption (pollutant of emerging concern) by taking different conditions of the pH, adsorbent mass, adsorbent calcination temperature and interferents into account. The results were compared with those obtained using bentonite immobilized in alginate beads. The acid medium has a greater efficiency for the caffeine adsorption and the adsorbent calcination temperature exerts, due to structural changes. Caffeine removal higher than 90% was obtained at optimized conditions. The Langmuir model indicated a better fit of the data and the adsorption capacity of caffeine onto bentonite. The bentonite immobilized led to a slower adsorption process in relation to the suspended.


Subject(s)
Water Pollutants, Chemical/isolation & purification , Bentonite/chemistry , Caffeine/chemistry , Thermodynamics , Caffeine/adverse effects , Adsorption , Environmental Pollutants/isolation & purification , Hot Temperature , Hydrogen-Ion Concentration , Models, Theoretical
18.
Braz. arch. biol. technol ; 63: e20190091, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132234

ABSTRACT

Abstract The kinetics and equilibrium of experimental data of mercury (II) sorption using three different macrophytes E. crassipes, E. azurea and S. ariculata were analyzed. From the kinetic models used, the model 1, which considers the surface area of constant sorption, presents the coefficient of determination, R2, closer to the unit (0.97). Already, in the liquid phase, the best fit of the experimental data was obtained for model 2 (R2=0.96), which considers the variable surface area. The calculated values for the determination coefficients indicate that the Redlich-Peterson isotherm best describes the equilibrium (R2=0.79). The results show that the macrophyte S. ariculata surface area, which presented the highest adsorption potential (15.77x10-4m2.g-1), was far below those found in the best adsorbents. However, considering the large volume of adsorbent material required in an industrial plant and the low cost of the analyzed adsorbents, it is considered that the macrophytes investigated have a considerable potential for the removal of mercury from wastewater.


Subject(s)
Macrophytes , Echinodermata/drug effects , Mercury/pharmacokinetics , Models, Biological , Adsorption
19.
Rev. bras. farmacogn ; 29(1): 69-76, Jan.-Feb. 2019. tab, graf
Article in English | LILACS | ID: biblio-990769

ABSTRACT

Abstract In this study, the adsorption/desorption characteristics of quercetin, luteolin and apigenin from Flos populi extract (Populus tomentosa Carrière, Salicaceae) on twelve macroporous resins (NKA-9, HPD-600, HPD-826, HPD-750, HPD-400, DM-130, AB-8, SP-825, X-5, D-101, HPD-100, HPD-200) were evaluated. Both high adsorption and desorption capacities of quercetin, luteolin and apigenin from Flos populi extract on SP-825 resin indicated that SP-825 resin was appropriate and its data were well fitted to the Langmuir and Freundlich isotherms. To get the optimal separation process, the influences of factors such as flow rates, loading sample volumes, concentrations of desorption solution were further investigated. Column packed with SP-825 resin was used to perform dynamic adsorption and desorption experiments. After one round of treatment, the contents of quercetin, luteolin and apigenin in the final products were 3.75-fold, 3.67-fold and 3.54-fold increased with recovery yields of 87.25, 85.19 and 82.22%, respectively. The results showed that the preparative enrichment of quercetin, luteolin and apigenin was available via adsorption and desorption on SP-825 resin. This method is a promising basis for the large-scale preparation of quercetin, luteolin and apigenin from Flos populi.


Subject(s)
Quercetin , Apigenin , Luteolin , Adsorption , Populus
20.
China Journal of Chinese Materia Medica ; (24): 40-47, 2019.
Article in Chinese | WPRIM | ID: wpr-771520

ABSTRACT

Affinity chromatography is characterized by its high specificity,high recovery rate and sensitivity,and it has been widely used in the selection of active ingredients of traditional Chinese medicine,separation and enrichment of low molecular weight sugars and protein peptides,research on mechanism of action and discovery of targets.This paper reviewed the application of affinity chromatography and its adsorption isotherm model,kinetic model and adsorption thermodynamic mechanism in the field of traditional Chinese medicine.This summarizes and provides thinking for comprehensive applications of affinity chromatography theory in the field of active ingredient screening,purification and medicine interaction.


Subject(s)
Adsorption , Chromatography, Affinity , Drugs, Chinese Herbal , Chemistry , Medicine, Chinese Traditional , Models, Theoretical
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