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1.
Rev. bras. queimaduras ; 16(2): 111-116, abr-jun2017.
Article in Portuguese | LILACS | ID: biblio-915132

ABSTRACT

OBJETIVO: Verificar as especificidades do processo cicatricial em queimaduras e identificar se as células-tronco derivadas do tecido adiposo (CTDA) podem se tornar aliadas em sua reparação. MÉTODO: Realizou-se uma revisão da literatura com consultas a Biblioteca Virtual de Saúde, Google Acadêmico, PubMed, Scientific Electronic Library Online, Mendeley catalog of academic literature, artigos e bibliografia especilizada em cicatrização, queimaduras, células-tronco derivadas do tecido adiposo publicados entre 2012 e 2016. RESULTADOS: As queimaduras se diferenciam de outras lesões pela intensidade da inflamação sistêmica e pela sobreposição das fases cicatriciais desorganizando-as. As CTDA são encontradas no estroma vascular do tecido adiposo e podem ser obtidas por lipoaspiração. Estas células podem interferir nas fases cicatriciais pela secreção de citocinas de crescimento, substâncias imunomoduladoras e anti-inflamatórias, assim como pela capacidade de diferenciação celular e homing. CONCLUSÃO: As queimaduras ocorrem na população mundial de forma preocupante, com características de morbidade e mortalidade. Os problemas sistêmicos e locais da reparação tecidual parecem ser atenuados pelas CTDA, sua abundante disponibilidade para o cultivo celular e suas habilidades as apontam como aliadas na recuperação das queimaduras.(AU)


OBJECTIVE: To evaluate the distinctions of the healing process in burns and identify how the adipose-tissue derived stem cells (ATSC) may become allies in this repair process. METHODS: Review of the literature was carried out with consultations at Virtual Health Library, Google Scholar, PubMed, Scientific Electronic Library Online and Mendeley catalog of academic literature and specialized bibliography in healing, burns and ATSC published between 2012 and 2016. RESULTS: Burns differentiate themselves from other lesions by the intensity of systemic inflammation and by the overlapping of the healing wound phases disorganizing them. The ATSC are found in the vascular stroma of adipose tissue and can be obtained by liposuction. These cells can interfere in the healing wound phases by the secretion of growth cytokines, immunomodulatory and anti-inflammatory substances and the ability of cell differentiation and homing. CONCLUSION: Burns occur in the world population in a worrisome way with characteristics of morbimortality. The problems of tissue repair seem to be attenuated by ATSC, their abundant availability for cell culture and their abilities point them as promising allies in healing of burn wound.(AU)


Objetivo: Verificar las especificidades del proceso cicatricial en quemaduras y identificar si las células-tronco derivadas del tejido adiposo (CTDA) pueden tornarse aliadas en su reparación. Método: Se realizó una revisión del literatura con consultas en Biblioteca Virtual de Salud, Google Académico, PubMed, Scientific Electronic Library online online, Mendeley catalog of academic literature, artículos y bibliografía especilizada, quemaduras, CTDA, publicados entre 2012 y 2016. Resultados: Las quemaduras se diferencian de otras lesiones por la intesidad de la inflamación sistémica y por la sobreposición de las fases cicatriciales desorganizándolas. Las CTDA son encontradas en el estroma vascular del tejido adiposo y pueden ser obtenidas por lipoaspiracion. Estas celulas pueden interferir en las fases cicatriciales por secrecion de citoquinas del crecimiento, sustancias inmunomoduladoras, anti inflamatorias y por la capacidad de diferenciacion celular e homing. Conclusión: Las quemaduras ocurren en la población mundial de forma preocupante con caracteristicas de morbimortalidad. Los problemas de la reparacion tecidual parecen ser atenuados por las CTDA, su abundante disponibilidad para el cultivo celular y sus habilidades las apuntan como aliadas en la recuperacion de las quemaduras.(AU)


Subject(s)
Humans , Wound Healing , Burns/therapy , Adipose Tissue , Adult Stem Cells/transplantation
2.
Rev. cuba. hematol. inmunol. hemoter ; 33(1): 1-13, ene.-mar. 2017.
Article in Spanish | LILACS, CUMED | ID: biblio-901070

ABSTRACT

La Medicina Regenerativa se apoya fundamentalmente en la terapia celular, en la administración de factores bioactivos, en la ingeniería de tejidos y en la terapia génica. Según su estado evolutivo, las células madre pueden clasificarse en embrionarias y adultas. Recientemente se obtuvieron células con características embrionarias mediante la reprogramación de células adultas que se llamaron células madre pluripotentes inducidas. Se han obtenido importantes avances con la terapia con células madre adultas que tienen notables ventajas sobre las embrionarias, pues su manipulación resulta más simple y se pueden obtener del propio individuo que va a ser tratado. Los resultados obtenidos en Cuba evidencian los beneficios que la terapia celular puede aportar en pacientes sin otras opciones terapéuticas y sobre todo bajo un estricto control científico(AU)


Regenerative Medicine is fundamentally based on cell therapy, administration of bioactive factors, tissue engineering and gene therapy. According to their evolutionary state, stem cells can be classified into embryonics and adults. Recently were obtained cells with embryonic characteristics by reprogramming adult cells and have been called induced pluripotent stem cells. Already have been made significant progress in cell therapy using adult stem cells, which have significant advantages over embryonic cells, because its handling is simpler, and can be obtained from the own individual to be treated. The results obtained in Cuba show the benefits that cell therapy can bring to patients with no other therapeutic options and especially under strict scientific control(AU)


Subject(s)
Humans , Male , Female , Adult Stem Cells/transplantation , Regenerative Medicine/methods
3.
Rev. méd. (La Paz) ; 22(1): 20-26, 2016. ilus
Article in Spanish | LILACS | ID: lil-797311

ABSTRACT

INTRODUCCIÓN: las úlceras por presión son producto de una necrosis isquémica en la piel y tejido subcutáneo, se presentan en pacientes inmovilizados por etiología diversa, y en estadios avanzados incrementan la mortalidad. La terapia celular con Células Madre Somáticas, que se diferencian y proliferan a células maduras funcionalmente normales, tiene la finalidad de reparar la función de tejidos lesionados. OBJETIVO: Evidenciar la eficacia de las Células Madre Somáticas de médula ósea en la reparación de úlceras crónicas por presión, como una posibilidad terapéutica en los tratamientos convencionales no exitosos. MÉTODO: Se estudió 4 pacientes con úlceras crónicas por presión en estadios avanzados y refractarias a tratamiento convencional. Se obtuvo Células Madre Somáticas de la médula ósea del esternón, y se procedió al sembrado de las mismas una vez por semana. Resultados.- Las Células Madre Somáticas sembradas en las úlceras crónicas se diferenciaron en tejido muscular, conjuntivo, subcutáneo y epitelial en un periodo comprendido entre 30 a 69 días. CONCLUSIÓN: la terapia celular con Células Madre Somáticas de médula ósea se constituye en una posibilidad terapéutica en úlceras crónicas de estadios avanzados y refractarias a tratamiento convencional.


INTRODUCTION: chronic pressure ulcers result from an ischemic necrosis of the skin and subcutaneous tissue. This type of ulcers occurs in patients physically restrained by different etiology and in advanced stages increase mortality. Cell therapy allows to regenerate the function of injured tissues by using somatic stem cells that differentiate and proliferate into mature cells on order to repair injured tissues. OBJECTIVE: to demonstrate the effectiveness ofusing Somatic Stem Cells obtained from bone marrow in chronic pressure ulcers treatment, as a likely therapeutic option to unsuccessful conventional treatments. METHOD: it was studied 4 patients with chronic pressure ulcers in advanced stages and refractory to conventional treatment. It was obtained Somatic Stem Cells from the bone marrow and followed a process of seeding on a weekly basis. RESULTS: somatic Stem Cells seeded in chronic pressure ulcers differentiated into muscle, connective, epithelial and subcutaneous tissues. The repair of injured tissues lasted between 30 to 69 days. CONCLUSION: cell therapy by using Somatic Stem Cells from bone marrow constitutes a therapeutic option in chronic ulcers of advanced stages and refractory to conventional treatment.


Subject(s)
Humans , Cell- and Tissue-Based Therapy , Chlorhexidine/administration & dosage , Adult Stem Cells/transplantation , Catheters
4.
Braz. J. Vet. Res. Anim. Sci. (Online) ; 53(4): 1-9, 2016. ilus, tab
Article in English | LILACS, VETINDEX | ID: biblio-875380

ABSTRACT

Fifteen adult rabbits were used to evaluate the repair of experimental common calcaneal tendon defects treated with glycerin-preserved canine carotid artery xenografts alone or associated with autologous mononuclear bone marrow cells (AMCs). Rabbits were submitted to daily clinical examination; implanted sites were analyzed under light microscopy within 15, 30 and 60 days of surgery. Pelvic limbs receiving xenografts associated with AMCs had better physical performance as well as higher collagen fiber, fibroblast, lymphocyte and new vessel counts at all postoperative time points considered. Glycerin-preserved canine carotid artery xenografts associated with AMCs constituted an effective method for common calcaneal tendon repair in rabbits.(AU)


Utilizou-se 15 coelhos adultos para avaliar o reparo de lesão do tendão calcanear comum com implante de artéria carótida de cães, preservada em glicerina, associado ou não a células mononucleares autólogas da medula óssea (CMAs). Os animais foram observados diariamente por meio de avaliações clínicas e o local do implante foi analisado sob microscopia de luz decorridos 15, 30 e 60 dias de pós-operatório. Notou-se em todos os períodos de observação, com o implante associado às CMAs, melhor desempenho físico dos membros pélvico e maior intensidade de fibras colágenas, fibroblastos e linfócitos e neovascularização. A utilização de xenoimplante de artéria carótida de cães preservada em glicerina associado à administração de células mononucleares da medula óssea foi eficiente no reparo do tendão calcanear comum de coelhos.(AU)


Subject(s)
Animals , Rabbits , Achilles Tendon/injuries , Adult Stem Cells/transplantation , Bone Marrow Transplantation/veterinary , Carotid Arteries/transplantation , Glycerol/therapeutic use , Transplantation, Autologous/veterinary
5.
Einstein (Säo Paulo) ; 13(4): 637-639, Oct.-Dec. 2015.
Article in Portuguese | LILACS | ID: lil-770506

ABSTRACT

ABSTRACT Spermatogonial stem cells, which exist in the testicles since birth, are progenitors cells of male gametes. These cells are critical for the process of spermatogenesis, and not able to produce mature sperm cells before puberty due to their dependency of hormonal stimuli. This characteristic of the reproductive system limits the preservation of fertility only to males who are able to produce an ejaculate. This fact puts some light on the increase in survival rates of childhood cancer over the past decades because of improvements in the diagnosis and effective treatment in pediatric cancer patients. Therefore, we highlight one of the most important challenges concerning male fertility preservation that is the toxic effect of cancer therapy on reproductive function, especially the spermatogenesis. Currently, the experimental alternative for fertility preservation of prepubertal boys is the testicular tissue cryopreservationfor, for future isolation and spermatogonial stem cells transplantation, in order to restore the spermatogenesis. We present a brief review on isolation, characterization and culture conditions for the in vitro proliferation of spermatogonial stem cells, as well as the future perspectives as an alternative for fertility preservation in prepubertal boys. The possibility of restoring male fertility constitutes a research tool with an huge potential in basic and applied science. The development of these techniques may be a hope for the future of fertility preservation in cases that no other options exist, e.g, pediatric cancer patients.


RESUMO As espermatogônias-tronco, presentes nos testículos desde o nascimento, são as células progenitoras dos gametas masculinos, e, desse modo, críticas para o processo de espermatogênese. Antes da puberdade, essas células não são capazes de produzir espermatozoides maduros, o que só ocorrerá após o estímulo hormonal. Essa característica do sistema reprodutivo limita a possibilidade de preservação da fertilidade apenas para homens capazes de produzir um ejaculado. Tal fato coloca em evidência o aumento nas taxas de sobrevivência de crianças com câncer nas últimas décadas, devido principalmente à melhora no diagnóstico e ao tratamento dos pacientes pediátricos. Dessa forma, destaca-se um dos mais importantes desafios relativos à preservação da fertilidade masculina, que é o efeito tóxico das terapias anticâncer para o sistema reprodutivo, especialmente a espermatogênese. Tendo isso em vista, a alternativa experimental atualmente estudada para a preservação da fertilidade de pacientes pré-púberes é a criopreservação de tecido testicular para futuro isolamento e transplante de espermatogônias-tronco, a fim de restabelecer a espermatogênese. Apresentamos aqui uma breve revisão sobre isolamento, caracterização e condições de cultivo para a proliferação de espermatogônias-tronco, bem como as futuras perspectivas, como alternativa para preservação da fertilidade de meninos pré-púberes. A possibilidade de restabelecer a fertilidade masculina é uma ferramenta de pesquisa com potencial enorme de uso na pesquisa básica e aplicada. O desenvolvimento dessas técnicas pode fornecer uma esperança futura de preservação de fertilidade nos casos em que não há nenhuma outra opção, como para os pacientes pediátricos de câncer.


Subject(s)
Child , Humans , Male , Adult Stem Cells/transplantation , Fertility Preservation/methods , Infertility, Male/therapy , Stem Cell Transplantation , Biomarkers , Cryopreservation/methods , Puberty , Primary Cell Culture/methods , Stem Cell Transplantation/trends
6.
São Paulo; s.n; 2015. [225] p. ilus, tab, graf.
Thesis in Portuguese | LILACS | ID: biblio-870781

ABSTRACT

Os efeitos benéficos associados à injeção intramiocárdica de células-tronco adultas, obtidos em roedores, não tem sido reproduzidos de modo consistente em modelos animais de grande porte e seres humanos. Neste trabalho testamos a hipótese que o transplante de células-tronco mesenquimais derivadas do tecido adiposo de porcos (pASC) aumenta a perfusão tecidual cardíaca em animais infartados e humanizados pelo tratamento com um inibidor da enzima conversora de angiotensina (iECA) e um ?-bloqueador. Os animais foram submetidos a oclusão da artéria coronária circunflexa esquerda (ACX) e 4 semanas após o IM, 4 grupos foram randomizados para receber injeção intramiocárdica de pASC nas doses de 1, 2 ou 4x10 ...


The beneficial effects associated with intramyocardial injection of adult stem cells in rodents have not been consistently reproduced in larger animals and humans. We evaluated the dose of porcine adipose-tissue derived mesenchymal stem cells (pASC) to increase cardiac tissue perfusion in pigs treated with ace-inhibitors and ?-blockers to mimic human management post-MI. Animals were subjected to LCx occlusion and 4 weeks after MI blinded randomized in 4 groups to receive intramyocardial injection of pASC (1, 2 and 4x10 ...


Subject(s)
Animals , Angiotensin II Type 1 Receptor Blockers/therapeutic use , Adult Stem Cells/transplantation , Myocardial Perfusion Imaging/methods , Myocardial Infarction/therapy , Angiotensin-Converting Enzyme Inhibitors/therapeutic use , Adipose Tissue , Enalapril/therapeutic use , Metoprolol/therapeutic use , Swine
7.
Arq. bras. cardiol ; 103(6): 521-529, 12/2014. tab, graf
Article in English | LILACS | ID: lil-732166

ABSTRACT

Background: Morbimortality in patients with dilated idiopathic cardiomyopathy is high, even under optimal medical treatment. Autologous infusion of bone marrow adult stem cells has shown promising preliminary results in these patients. Objective: Determine the effectiveness of autologous transplantation of bone marrow adult stem cells on systolic and diastolic left ventricular function, and on the degree of mitral regurgitation in patients with dilated idiopathic cardiomyopathy in functional classes NYHA II and III. Methods: We administered 4,54 x 108 ± 0,89 x 108 bone marrow adult stem cells into the coronary arteries of 24 patients with dilated idiopathic cardiomyopathy in functional classes NYHA II and III. Changes in functional class, systolic and diastolic left ventricular function and degree of mitral regurgitation were assessed after 3 months, 6 months and 1 year. Results: During follow-up, six patients (25%) improved functional class and eight (33.3%) kept stable. Left ventricular ejection fraction improved 8.9%, 9.7% e 13.6%, after 3, 6 and 12 months (p = 0.024; 0.017 and 0.018), respectively. There were no significant changes neither in diastolic left ventricular function nor in mitral regurgitation degree. A combined cardiac resynchronization and implantable cardioversion defibrillation was implanted in two patients (8.3%). Four patients (16.6%) had sudden death and four patients died due to terminal cardiac failure. Average survival of these eight patients was 2.6 years. Conclusion: Intracoronary infusion of bone marrow adult stem cells was associated with an improvement or stabilization of functional class and an improvement in left ventricular ejection fraction, suggesting the efficacy of this intervention. There were no significant changes neither in left ventricular diastolic function nor in the degree of mitral regurgitation. .


Fundamento: Pacientes portadores de cardiomiopatia dilatada idiopática apresentam alta morbimortalidade, mesmo em tratamento clínico otimizado. A infusão autóloga de células-tronco adultas da medula óssea mostrou resultados clínicos preliminares promissores nesses pacientes. Objetivo: Determinar a eficácia do transplante autólogo de células-tronco adultas da medula óssea sobre as funções sistólica e diastólica, e o grau de insuficiência mitral em pacientes portadores de cardiomiopatia dilatada idiopática em classes funcionais NYHA II e III. Métodos: Infundiram-se 4,54 x 108 ± 0,89 x 108 células-tronco adultas da medula óssea nas artérias coronárias de 24 pacientes com cardiomiopatia dilatada idiopática em classes funcionais NYHA II e III. Após 3 meses, 6 meses e 1 ano, avaliaram-se as mudanças de classe funcional, das funções ventricular esquerda sistólica e diastólica, e do grau da insuficiência mitral. Resultados: No seguimento, seis (25%) pacientes melhoraram sua classe funcional e oito (33,3%) mantiveram sua classe funcional inicial. A fração de ejeção ventricular esquerda aumentou 8,9%, 9,7% e 13,6%, após 3 e 6 meses e 1 ano (p = 0,024; p = 0,017 e p = 0,018), respectivamente. A função diastólica ventricular esquerda e o grau de insuficiência mitral não demonstraram mudanças significativas. Dois pacientes (8,3%) receberam cardioversor e ressincronizador implantável. Ocorreram quatro (16,6%) mortes súbitas e quatro (16,6%) mortes por insuficiência cardíaca terminal. A sobrevida média desses oitos pacientes foi de 2,6 anos. Conclusão: A infusão intracoronariana de células-tronco adultas da medula óssea em pacientes com cardiomiopatia dilatada idiopática promoveu melhora ...


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Adult Stem Cells/transplantation , Bone Marrow Transplantation/methods , Cardiomyopathy, Dilated/surgery , Bone Marrow Transplantation/mortality , Cardiomyopathy, Dilated/mortality , Cardiomyopathy, Dilated , Coronary Vessels/surgery , Diastole/physiology , Echocardiography, Doppler , Follow-Up Studies , Kaplan-Meier Estimate , Mitral Valve Insufficiency/physiopathology , Reproducibility of Results , Statistics, Nonparametric , Systole/physiology , Time Factors , Treatment Outcome , Transplantation, Autologous/methods , Transplantation, Autologous/mortality , Ventricular Function, Left/physiology
8.
Rev. cuba. hematol. inmunol. hemoter ; 29(1): 16-23, ene.-mar. 2013.
Article in Spanish | LILACS | ID: lil-666710

ABSTRACT

Se describen los aspectos más relevantes de las células madre mesenquimales que incluyen su plasticidad hacia diversos linajes celulares, como condrocitos, osteocitos y adipositos, entre otros. Dichas células se pueden obtener principalmente de médula ósea, sangre de cordón umbilical y tejido adiposo, de donde se han logrado establecer cultivos que permiten estudiar sus propiedades funcionales y fenotípicas. Estas expresan antígenos CD73, CD90 y CD105 en ausencia de antígenos hematopoyéticos CD34 y CD45, así como diferentes receptores para citocinas y moléculas de adhesión. Futuras investigaciones permitirán conocer diversos aspectos biológicos de estas células, de importancia en su utilización en la medicina regenerativa como medida terapéutica para solucionar una amplia variedad de enfermedades


The main features of multipotent mesenchymal stem cells are described, which includes its plasticity to various cell lineages such as chondrocytes, osteocytes and adipocytes, among others. These cells can be obtained mainly from bone marrow, umbilical cord blood and adipose tissue, where it has been possible to establish crops that allow the study of their functional and phenotypic properties. They express antigens CD73, CD 90 and CD105 in the absence of CD34 and CD45 hematopoietic antigens, as well as cytokine receptors and adhesion molecules. Future investigations will make it possible to know various biological aspects of these cells of importance for their use in regenerative medicine as a therapeutic mean to solve a wide variety of diseases


Subject(s)
Humans , Adult Stem Cells/transplantation , Stem Cell Research/history , Regenerative Medicine/history , Regenerative Medicine/methods
9.
Indian J Hum Genet ; 2012 May; 18(2): 150-154
Article in English | IMSEAR | ID: sea-143262

ABSTRACT

In recent years, the field of dentistry has embossed its presence by taking major leaps in research and further bringing it into practice. The most valuable ongoing research in regenerative dentistry is the study on stem cells. It was instituted that stem cells grow rapidly and have the potential to form specialized dentin, bone, and neuronal cells. These neuronal cells can be used for dental therapies and can provide better treatment options for patients. The stem cells based therapies could help in new advances in treating damaged teeth, inducing bone regeneration and treating neural injury as well.


Subject(s)
Adult Stem Cells/transplantation , Dentistry/methods , Dentistry/trends , Embryonic Stem Cells/transplantation , Humans , Regenerative Medicine , Stem Cell Transplantation
10.
Acta cir. bras ; 27(3): 201-209, Mar. 2012. ilus, tab
Article in English | LILACS | ID: lil-617958

ABSTRACT

PURPOSE: To evaluate the effects of mesenchymal stem cells (MSC) from eight mice C57BL/6 gfp+ bone marrows expanded in cultures associated with platelets rich plasma (PRP) deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice C57BL/6. METHODS: The animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. Control group did not receive treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL associated with 50.0µL of plasma gel containing 1.0 x 10(9) autologous platelets within the defect. RESULTS: In the treated group was observed process of angiogenesis and bone repair better than control group. CONCLUSION: Mesenchymal stem cells derived from bone marrow of C57BL/6 gfp+ mice associated with PRP gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.


OBJETIVO: Avaliar os efeitos da associação das células-tronco mesenquimais (MSC) oriundas da medula óssea de oito camundongos jovens C57BL/6 gfp+ e expandidas em culturas, com Plasma Rico em Plaquetas (PRP) provenientes de outros oito camundongos, na reparação de defeitos críticos confeccionados em calvária de 24 camundongos adultos C57BL/6. MÉTODOS: Os animais foram submetidos a um defeito craniano de 6,0mm de diâmetro e separados em dois grupos experimentais iguais. O grupo controle não recebeu tratamento e no grupo tratado foi administrado, no interior do defeito, pellet de MSC contendo 1,0 x 10(7) células/mL associado com 50,0µL de plasma em gel autólogo contendo 1,0 x 10(9) plaquetas. RESULTADOS: No grupo tratado verificou-se processo de angiogênese e reparação óssea superior ao grupo controle. CONCLUSÃO: A associação das células-tronco mesenquimais (MSC) derivadas da medula óssea de camundongos C57BL/6 gfp+ com gel de PRP aplicadas em defeitos ósseos críticos confeccionadas em calvária de camundongos C57BL/6 jovens, contribuiu positivamente para o processo de reparação óssea.


Subject(s)
Animals , Male , Mice , Adult Stem Cells/transplantation , Bone Marrow Cells/physiology , Bone Regeneration/physiology , Mesenchymal Stem Cells , Mesenchymal Stem Cell Transplantation/methods , Platelet-Rich Plasma/physiology , Skull/surgery , Adult Stem Cells/ultrastructure , Bone Marrow Cells/ultrastructure , Cells, Cultured , Disease Models, Animal , Green Fluorescent Proteins/genetics , Mesenchymal Stem Cells , Mice, Transgenic , Osteogenesis/physiology , Random Allocation , Skull/injuries , Skull/ultrastructure , Transplantation, Homologous , Tissue Engineering/methods
11.
Rev. bras. cir. plást ; 26(3): 394-401, July-Sept. 2011. ilus, tab
Article in English, Portuguese | LILACS | ID: lil-608195

ABSTRACT

BACKGROUND: The differences between fetal and adult scars suggest the possibility of manipulating skin scarring outcomes. This study aimed to assess whether the use of adult stem cells from adipose tissue is beneficial to skin healing. METHODS: This was a randomized controlled study for which 18 patients were selected based on inclusion and exclusion criteria. The adult stem cells used were autologous and were extracted from infraumbilical adipose tissue prior to abdominoplasty. These cells were implanted into the surgical wound dermis in the suprapubic region before skin synthesis. The results were assessed blindly based on the Draaijers scale by three physicians and by the patients themselves in a self-assessment. Photometric assessment by digital photography was also performed. RESULTS: Among the 18 operated patients, considering the surgical result, 17 (94.4 percent) had excellent or good results and one (5.5 percent) had wound dehiscence, which was considered a bad result. Considering skin healing in the searched area, there was no statistically significant difference in the photometric evaluation; in both the self-assessment by the patients and the physicians' assessment, the results were significantly in favor of intervention with stem cells (P = 0.12 and P = 0.003, respectively). Consideration of all assessments (physicians, patients and photometric) found a statistically significant difference in favor of the implantation of adult stem cells from adipose tissue (P <0.001). CONCLUSIONS: Skin healing results after implantation of adult stem cells derived from adipose tissue were satisfactory.


INTRODUÇÃO: Fatores que diferenciam a cicatrização fetal e a do adulto instigam a possibilidade de manipulação das soluções de continuidade da pele. Este estudo teve como objetivo avaliar se o uso de células-tronco adultas do tecido adiposo é benéfico à cicatrização da pele. MÉTODO: Estudo controlado, randomizado, para o qual foram selecionadas 18 pacientes, considerando-se critérios de inclusão e exclusão. As células-tronco adultas utilizadas eram autólogas, extraídas do tecido adiposo da região infraumbilical, precedendo a realização da abdominoplastia. Essas células, antes da síntese da pele, foram implantadas na derme da ferida operatória, na região suprapúbica. A avaliação dos resultados foi realizada com base na escala de Draaijers, por três avaliadores médicos cegados, e pelas próprias pacientes, por autoavaliação. Foi realizada, também, avaliação fotométrica por fotografia digital. RESULTADOS: Dentre as 18 pacientes operadas, sob o ponto de vista cirúrgico, 17 (94,4 por cento) apresentaram resultados excelentes ou bons e uma (5,5 por cento) apresentou deiscência de sutura, considerado mau resultado. Quanto à cicatrização da pele na área pesquisada, à avaliação fotométrica, não houve diferença estatisticamente significante; à autoavaliação pelas pacientes, os resultados atingiram nível de significância a favor da intervenção com células-tronco (P = 0,12); e à avaliação pelos médicos, foi atingido nível de significância a favor da intervenção por células-tronco (P = 0,003). Considerando-se todas as avaliações realizadas (médicos, pacientes e fotométrica), foi encontrada diferença estatisticamente significante favorável ao implante de células-tronco adultas do tecido adipose (P < 0,001). CONCLUSÕES: Os resultados da cicatrização da pele, após implante de células-tronco adultas derivadas de tecido adiposo, foram satisfatórios.


Subject(s)
Humans , Female , Adult , Middle Aged , History, 21st Century , Photometry , Skin , Wound Healing , Adipose Tissue , Evaluation Study , Abdomen , Adult Stem Cells , Abdominoplasty , Photometry/methods , Skin/injuries , Adipose Tissue/surgery , Adipose Tissue/transplantation , Adult Stem Cells/transplantation , Abdominoplasty/methods , Abdomen/surgery
12.
Journal of Forensic Medicine ; (6): 206-209, 2010.
Article in Chinese | WPRIM | ID: wpr-983568

ABSTRACT

Neurogenic erectile dysfunction (NED) commonly results from erectile nerve damage. Recent researches have focused on the preclinical study of stem cell-based therapies targeted at repairing and protecting nervi erigentes. In this paper, researches of NESCs, MDSCs, ASCs and MSCs in NED are reviewed. Early studies have demonstrated that stem cells and gene modified stem cells were effective to the therapy of ED, even likely to cure ED. Stem cells are expected to be applied in the clinical therapy of NED. Stem cells as a new therapy technique will bring up a new challenge in forensic clinical medicine.


Subject(s)
Animals , Humans , Male , Adipose Tissue/cytology , Adult Stem Cells/transplantation , Embryonic Stem Cells/transplantation , Erectile Dysfunction/therapy , Mesenchymal Stem Cell Transplantation , Nerve Regeneration , Nervous System Diseases/complications , Penis/innervation , Peripheral Nerve Injuries , Stem Cell Transplantation
13.
Arq. bras. oftalmol ; 72(2): 254-256, mar.-abr. 2009. ilus
Article in Portuguese | LILACS | ID: lil-513901

ABSTRACT

Paciente apresentou falência de transplante de limbo e conjuntiva de doador vivo alógeno no olho direito após ceratoconjuntivite epidêmica. Após alguns meses, foi submetida a transplante de células-tronco epiteliais límbicas alógenas cultivadas ex vivo sobre membrana amniótica (primeiro caso no Brasil), tendo evoluído com epitelização total da córnea e melhora da acuidade visual. Após o 3º mês da cirurgia, iniciou-se neovascularização superficial periférica com piora da transparência corneana. A visão manteve-se 0,1 após um ano de cirurgia.


Case report of a patient who developed failure of an allogenic living related conjunctival limbal transplantation in the right eye after an episode of epidemic keratoconjunctivitis. After a few months, she underwent transplantation of allogenic limbal epithelial stem cells cultivated ex vivo on amniotic membrane (first case in Brazil). The patient evolved with total corneal epithelialization and improvement of the visual acuity. Three months after the surgery, peripheral superficial neovascularization with worsening of the corneal transparency was observed. The vision remained 0.1 after one year of the transplantation.


Subject(s)
Female , Humans , Male , Middle Aged , Amnion , Adult Stem Cells/transplantation , Epithelial Cells/transplantation , Keratoconjunctivitis/surgery , Limbus Corneae/cytology , Cell Culture Techniques/methods , Transplantation, Homologous , Treatment Outcome
14.
Salud pública Méx ; 49(6): 437-444, nov.-dic. 2007. ilus
Article in Spanish | LILACS | ID: lil-470755

ABSTRACT

La lesión medular (LM) es un problema que afecta sobre todo a la población en edad laboral y, por lo tanto, sus repercusiones rebasan el ámbito familiar. La LM es irreversible para la mitad de las víctimas y en la actualidad los tratamientos existentes consisten en la asistencia y la estabilización espinal. Con el reconocimiento de la existencia de células madre (CM), el tratamiento de la LM ha recibido otro enfoque. Las CM se encargan de la renovación de los tejidos durante la vida del individuo y su reparación en caso de lesión. Las CM más atractivas desde el punto de vista terapéutico son las capaces de generar diversos tejidos, obtenibles con facilidad, y cuya manipulación es aceptable en términos éticos. En este artículo se presentan algunos de los estudios realizados con CM de diversos orígenes y su aplicación al tratamiento de la LM.


Spinal cord injury (SCI) is a trauma problem striking mainly working age adults, therefore affecting society beyond the victim’s family circle. Most of the victims of SCI will never recover; therapy for this type of injury consists basically on spinal cord support and stabilization. With the discovery of stem cells (SC), SCI treatment has been given another chance. Stem cells are responsible for tissue renewal throughout the individual’s life, as well as tissue repair when needed. From the therapeutic point of view, the most appealing SC are those capable of generating a variety of tissues, those easily harvested, and finally, those ethically unquestioned. This article summarizes some studies carried with SC of various origins and their application to SCI treatment.


Subject(s)
Adult , Aged , Animals , Female , Humans , Male , Middle Aged , Rats , Spinal Cord Injuries/rehabilitation , Adult Stem Cells/cytology , Adult Stem Cells/transplantation , Brain/cytology , Nerve Regeneration , Quadriplegia/etiology , Quadriplegia/rehabilitation , Quadriplegia/surgery , Spinal Cord Injuries/surgery , Spinal Cord/cytology , Stem Cell Transplantation , Stem Cells/classification
15.
Article in English | IMSEAR | ID: sea-22837

ABSTRACT

Cell transplantation has been proposed to replace lost neurons in the diseased brain, and after injuries to the central nervous system (CNS). Strategies for cellular therapy in the CNS consist primarily in heterologous transplantations. Despite the CNS being an immunologically privileged site, immune rejection of intracerebral transplants remains a concern. In addition, the use of immunosuppressive drugs, like cyclosporine, is a major constraint associated with heterologous transplantations. Autologous transplantation is therefore viewed as the model of choice for cellular therapy. With the recent progress in somatic cell nuclear transfer (SCNT) research, and the confirmation that neurogenesis occurs in the adult brain and neural stem cells (NSCs) reside in the adult CNS, new opportunities for autologous transplantations are being considered for the CNS, and are promising.


Subject(s)
Adult Stem Cells/transplantation , Animals , Central Nervous System Diseases/therapy , Embryonic Stem Cells/transplantation , Fibroblasts/transplantation , Humans , Neuronal Plasticity , Neurons/transplantation , Nuclear Transfer Techniques , Olfactory Bulb/cytology , Stem Cell Transplantation , Transplantation, Autologous
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