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1.
An. acad. bras. ciênc ; 89(3): 1785-1799, July-Sept. 2017. tab, graf
Article in English | LILACS | ID: biblio-886736

ABSTRACT

ABSTRACT The effect of the ethanolic extract of propolis (EEP) on Aeromonas hydrophila was analyzed by determination of minimum inhibitory concentration (MIC). Then, the effects of crude propolis powder (CPP) on growth, hemato-immune parameters of the Nile tilapia, as well as its effects on resistance to A. hydrophila challenge were investigated. The CPP (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%) was added to the diet of 280 Nile tilapia (50.0 ± 5.7 g fish-1). Hemato-immune parameters were analyzed before and after the bacterial challenge. Red blood cell, hematocrit, hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and hydrogen peroxide (H2O2) and nitric oxide (NO) were evaluated. The MIC of the EEP was 13% (v/v) with a bactericidal effect after 24 hours. Growth performance was significantly lower for those fish fed diets containing 2.5 and 3% of CPP compared to the control diet. Differences in CPP levels affected fish hemoglobin, neutrophils number and NO following the bacterial challenge. For others parameters no significant differences were observed. Our results show that although propolis has bactericidal properties in vitro, the addition of crude propolis powder to Nile tilapia extruded diets does not necessarily lead to an improvement of fish health.


Subject(s)
Animals , Propolis/administration & dosage , Gram-Negative Bacterial Infections/veterinary , Aeromonas hydrophila/drug effects , Cichlids/microbiology , Fish Diseases/diet therapy , Propolis/therapeutic use , Microbial Sensitivity Tests , Gram-Negative Bacterial Infections/diet therapy , Gram-Negative Bacterial Infections/microbiology , Dietary Supplements , Cichlids/blood , Fish Diseases/microbiology , Immunity, Innate
2.
An. acad. bras. ciênc ; 89(1): 203-212, Jan,-Mar. 2017. tab, graf
Article in English | LILACS | ID: biblio-886624

ABSTRACT

ABSTRACT Linalool is the main compound of many essential oils and occurs in two isomeric forms: S-(+)- and R-(-)-linalool. This study aimed to determine if linalool isomers have different antimicrobial and anesthetic properties in fish. For this purpose, these compounds were previously isolated from Lippia alba (Mill.)N. E. Brown and Ocimum americanum L. essential oils. Antimicrobial effects were evaluated through the microdilution test against Aeromonas hydrophila, an important fish disease etiologic agent. Induction time until sedation, anesthesia and recovery time were determined in silver catfish (Rhamdia quelen) through bath exposure (60, 180, 300 or 500 μL L-1). The results showed different biological properties for the isomers being S-(+)-linalool the only active against A. hydrophila at 3.2 mg mL-1. The sedation was induced without differences between the compounds, however R-(-)-linalool promoted faster anesthesia. There were no differences regarding the recovery time of the animals exposed to the linalool isomers. Although both S-(+)- and R-(-)-linalool can be used for sedative purposes, their use in A. hydrophila infection is inadvisable due to the high effective concentration. Considering anesthesia as the main objective, the R-(-)-linalool demonstrated clear advantages at lower concentration.


Subject(s)
Animals , Catfishes , Aeromonas hydrophila/drug effects , Monoterpenes/pharmacology , Hypnotics and Sedatives/pharmacology , Anesthetics/pharmacology , Anti-Bacterial Agents/pharmacology , Reference Values , Stereoisomerism , Time Factors , Oils, Volatile/chemistry , Microbial Sensitivity Tests , Reproducibility of Results , Ocimum/chemistry , Lippia/chemistry , Monoterpenes/isolation & purification , Monoterpenes/chemistry , Acyclic Monoterpenes
3.
Braz. j. microbiol ; 44(4): 1189-1194, Oct.-Dec. 2013. ilus, tab
Article in English | LILACS | ID: lil-705283

ABSTRACT

The aim of this study was to assess the activity of essential oil extracted from the leaves of C. blanchetianus Baill, popularly known as "marmeleiro", in inhibiting the growth and survival of pathogenic microorganisms in food by determining their survival in vitro and by observing the behaviour of Listeria monocytogenes inoculated into a food model (meat cubes) that was stored at refrigeration temperature (7 ± 1 ºC) for 4 days. The results indicated a bactericidal effect against Aeromonas hydrophila and Listeria monocytogenes and bacteriostatic action against Salmonella Enteritidis. A bacteriostatic effect on meat contaminated with L. monocytogenes was found for all concentrations of essential oils tested. These results showed that essential oil from the leaves of C. blanchetianus Baill represents an alternative source of potentially natural antimicrobial agents that may be used as a food preservative.


Subject(s)
Aeromonas hydrophila/drug effects , Anti-Bacterial Agents/pharmacology , Croton Oil/pharmacology , Croton/chemistry , Listeria monocytogenes/drug effects , Oils, Volatile/pharmacology , Salmonella enteritidis/drug effects , Anti-Bacterial Agents/isolation & purification , Bacterial Load , Croton Oil/isolation & purification , Food Preservatives/isolation & purification , Food Preservatives/pharmacology , Microbial Viability/drug effects , Oils, Volatile/isolation & purification , Plant Leaves/chemistry , Temperature
5.
Article in English | WPRIM | ID: wpr-197814

ABSTRACT

This study aimed to compare the clinical presentations of Aeromonas hydrophila, A. veronii biovar sobria and A. caviae monomicrobial bacteremia by a retrospective method at three hospitals in Taiwan during an 8-yr period. There were 87 patients with A. hydrophila bacteremia, 45 with A. veronii biovar sobria bacteremia and 22 with A. caviae bacteremia. Compared with A. hydrophila and A. veronii biovar sobria bacteremia, A. caviae bacteremia was more healthcare-associated (45 vs 30 and 16%; P = 0.031). The patients with A. caviae bacteremias were less likely to have liver cirrhosis (27 vs 62 and 64%; P = 0.007) and severe complications such as shock (9 vs 40 and 47%; P = 0.009) and thrombocytopenia (45 vs 67 and 87%; P = 0.002). The APACHE II score was the most important risk factor of Aeromonas bacteremia-associated mortalities. The APACHE II scores of A. caviae bacteremias were lower than A. hydrophila bacteremia and A. veronii biovar sobria bacteremia (7 vs 14 and 16 points; P = 0.002). In conclusion, the clinical presentation of A. caviae bacteremia was much different from A. hydrophila and A. veronii biovar sobria bacteremia. The severity and mortality of A. caviae bacteremia were lower than A. hydrophila or A. veronii biovar sobria bacteremia.


Subject(s)
APACHE , Adult , Aeromonas caviae/drug effects , Aeromonas hydrophila/drug effects , Aged , Aged, 80 and over , Bacteremia/complications , Cross Infection/microbiology , Female , Gram-Negative Bacterial Infections/complications , Humans , Liver Cirrhosis/microbiology , Male , Middle Aged , Retrospective Studies , Shock, Septic/microbiology , Taiwan , Thrombocytopenia/complications , Young Adult
6.
EMHJ-Eastern Mediterranean Health Journal. 2005; 11 (3): 425-434
in English | IMEMR | ID: emr-156771

ABSTRACT

To investigate the inactivation of potential pathogens, we evaluated survival rates for Escherichia coli, Enterococcus faecalis, Aeromonas hydrophila and Salmonella typhi in samples taken from the Isfahan drinking water system. Chlorine residual, pH, temperature and total organic carbon levels were measured. The organism most sensitive to chlorine was A. hydrophila. It was inactived in < 100 minutes at chlorine levels of 0.11 mg/L to 0.90 mg/L. The other 3 organisms showed higher resistance. E. coli tolerated 0.30 mg/L chlorine for > 1000 minutes while Ent. faecalis and S. typhi survived at total chlorine concentration of 0.50 mg/L for 100 minutes. We concluded that total chlorine levels of less than 0.71 mg/L in water supply systems cannot provide the recommended safety levels


Subject(s)
Humans , Aeromonas hydrophila/drug effects , Carbon/analysis , Colony Count, Microbial , Enterococcus faecalis/drug effects , Escherichia coli/drug effects
7.
J Environ Biol ; 2003 Oct; 24(4): 373-9
Article in English | IMSEAR | ID: sea-113753

ABSTRACT

The influence of environmental factors on the growth of Aeromonas hydrophila was investigated Four isolates (AH 37, AH 79, AH 86 and AH 100) were exposed to various environmental factors such as pH, salt concentration and temperature in laboratory condition. All the four isolates showed more or less similar growth at pH 7.0, 8.0 and 9.0 at 30 degrees C and 5 degrees C. At pH 5.0, 6.0 and 10.0, the log number of cells was found to be lesser than that of pH 7.0, 8.0 and 9.0 at both 30 degrees C and 5 degrees C. The results of the influence of salt concentration on the growth of Aeromonas hydrophila revealed that NaCl concentration of 0.5%, 1.0% and 2.0% favored the growth of this organism at both 30 degrees C and 5 degrees C. Increase in the salt concentration resulted in the growth of the decrease of this organism. Three percentages and 4% salt concentration moderately supported the growth of the organisms in the medium whereas at 5.0% NaCl concentration, there was no growth. Moderate growth of A. hydrophila at 5 degrees C is an interesting observation. The ability to grow at salt concentration between 0.5%, 4.0% under acidic and alkaline conditions pose a problem in the preservation of seafoods. These criteria may account for modified preservation techniques.


Subject(s)
Aeromonas hydrophila/drug effects , Colony Count, Microbial , Hydrogen-Ion Concentration , Sodium Chloride/pharmacology , Temperature , Time Factors
8.
Ciênc. rural ; 30(4): 661-4, jul.-ago. 2000. tab
Article in Portuguese | LILACS | ID: lil-273894

ABSTRACT

Com o objetivo da caracterizaçäo da ocorrência de ®red leg¼ em Minas Gerais, avaliaram-se räs jovens e adultas que apresentavam úlceras nas patas e dedos, derrame hemorrágico na pele da regiäo ventral, incoordenaçäo motora, altas taxas de mortalidade em adultos e abscessos hepáticos à necropsia. No cultivo de fragmentos de pele lesada e abscessos, foi isolado e identificado Aeromonas hydrophila. O efeito bactericida do VANTOCIL IB foi superior ao permanganato de potássio, determinado pela concentraçäo inibitória mínima para colônias isoladas.


Subject(s)
Animals , Aeromonas hydrophila/drug effects , Aeromonas hydrophila/isolation & purification , Biguanides/therapeutic use , In Vitro Techniques , Gram-Negative Bacterial Infections/drug therapy , Gram-Negative Bacterial Infections/veterinary , Ranidae/injuries , Ranidae/microbiology
9.
Indian J Exp Biol ; 1999 Nov; 37(11): 1097-100
Article in English | IMSEAR | ID: sea-60202

ABSTRACT

Aquaneem, an emulsified product prepared from the neem (A. indica) kernel was tested against four pathogenic bacteria of fish (i.e. Aeromonas hydrophila, Pseudomonas fluorescens, Escherichia coli and Myxobacteria spp.) to test its efficacy as an antibacterial agent. Growth inhibitory property of the product at 10, 15 and 20 ppm has been noticed and recorded. The percentage reduction of bacterial cell population was noted to be maximum on 9th day at 20 ppm concentration (i.e. 70.14%, 74.15% and 61.75% for A. hydrophila, P. fluorescens and E. coli respectively) with the only exception of myxobacteria which showed maximum reduction percentage (63.90%) on 15th day. Among all the bacteria tested A. hydrophila, P. fluorescens and Myxobacteria spp. exhibited maximum sensitivity to Aquaneem in terms of percentage reduction of bacterial cell population in comparison to E. coli.


Subject(s)
Aeromonas hydrophila/drug effects , Animals , Anti-Bacterial Agents/pharmacology , Escherichia coli/drug effects , Fishes/microbiology , Myxococcales/drug effects , Plant Extracts/pharmacology , Plants, Medicinal , Pseudomonas fluorescens/drug effects
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