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1.
Rev. Eugenio Espejo ; 15(2): 115-136, 20210516.
Article in Spanish | LILACS | ID: biblio-1252504

ABSTRACT

Blastocystis es un stramenopile o cromista, pleomórfico no móvil. Se han identificado diecinue-ve subtipos de este organismo (ST1-ST19). Tiene una presencia a nivel mundial. Este microor-ganismo tiene un metabolismo intermediario anaeróbico. Un aspecto interesante de la bioquími-ca de este stramenopile está dado por la presencia de organelas similares a mitocondrias con un conjunto de rutas: cadena de fosforilación oxidativa incompleta, ciclo de Krebs parcial, metabo-lismo de ácidos grasos (anabolismo y catabolismo), metabolismo de aminoácidos y ensamblaje de proteínas con centros hierro/azufre. El tratamiento se ha basado tradicionalmente en metroni-dazol y otros imidazoles. Sin embargo, hay un número creciente de cepas resistentes a esos medicamentos. La reciente obtención del genoma nuclear y los estudios bioquímicos, proteómi-cos, metabolómicos, interactómicos permitirán el desarrollo racional de nuevos fármacos curati-vos. El objetivo de esta revisión es describir el metabolismo de Blastocystis spp


Blastocystis is a stramenopile or chromist, nonmobile pleomorphic. Nineteen subtypes of this organism (ST1-ST19) have been identified worldwide. This microorganism has an intermediate anaerobic metabolism. An interesting aspect of the biochemistry of this stramenopile is given by the presence of mitochondrial-like organelles with a set of pathways: incomplete oxidative phos-phorylation chain, partial Krebs cycle, fatty acid metabolism (anabolism and catabolism), amino acid metabolism and protein assembly with iron / sulfur centers. Treatment has traditionally been based on metronidazole and other imidazoles. However, there are a growing number of strains resistant to these drugs. The recent obtaining of the nuclear genome and the biochemical, proteomic, metabolomic and interactomic studies will allow the rational development of new curative drugs. The objective of this review is to describe the metabolism of Blastocystis spp.


Subject(s)
Humans , Male , Female , Parasitic Diseases , Blastocystis , Metabolism , Anaerobiosis , Metronidazole , Antigens, Protozoan
2.
Electron J Biotechnol ; 49: 34-41, Jan. 2021. tab, graf
Article in English | LILACS | ID: biblio-1291638

ABSTRACT

BACKGROUND: This work studied how the exposure to an unusual substrate forced a change in microbial populations during anaerobic fermentation of crude glycerol, a by-product of biodiesel production, with freshwater sediment used as an inoculum. RESULTS: The microbial associations almost completely (99.9%) utilized the glycerol contained in crude glycerol 6 g L 1 within four days, releasing gases, organic acids (acetic, butyric) and alcohols (ethanol, n-butanol) under anaerobic conditions. In comparison with control medium without glycerol, adding crude glycerol to the medium increased the amount of ethanol and n-butanol production and it was not significantly affected by incubation temperature (28 C or 37 C), nor incubation time (4 or 8 d), but it resulted in reduced amount of butyric acid. Higher volume of gas was produced at 37 C despite the fact that the overall bacterial count was smaller than the one measured at 20 C. Main microbial phyla of the inoculum were Actinobacteria, Proteobacteria and Firmicutes. During fermentation, significant changes were observed and Firmicutes, especially Clostridium spp., began to dominate, and the number of Actinobacteria and Gammaproteobacteria decreased accordingly. Concentration of Archaea decreased, especially in medium with crude glycerol. These changes were confirmed both by culturing and culture-independent (concentration of 16S rDNA) methods. CONCLUSIONS: Crude glycerol led to the adaptation of freshwater sediment microbial populations to this substrate. Changes of microbial community were a result of a community adaptation to a new source of carbon.


Subject(s)
Bacteria/isolation & purification , Geologic Sediments/microbiology , Fresh Water/microbiology , Glycerol/metabolism , Bacteria/metabolism , Adaptation, Biological , Biofuels , Fermentation , Real-Time Polymerase Chain Reaction/methods , Anaerobiosis
3.
Chinese Journal of Biotechnology ; (12): 3565-3577, 2021.
Article in Chinese | WPRIM | ID: wpr-921448

ABSTRACT

The genus Dehalogenimonas (Dhgm) is a recently discovered taxonomic group within the class Dehalococcoidia of the phylum Chloroflexi. To date, Dhgm consists of three formally described species including Dehalogenimonas lykanthroporepellens, Dehalogenimonas alkenigignens and Dehalogenimonas formicexedens. All isolates of these three Dhgm species are obligate organohalide-respiring bacteria. They use hydrogen and formate as electron donors and chlorinated ethanes (e.g., 1,2,3-trichloropropane, 1,2-dichloropropane, 1,2-dichloroethane) as electron acceptors in energy-conserving reductive dechlorination reaction. Chlorinated ethanes are common groundwater contaminants in China. The unique metabolic capacities of Dhgm strains implicate it may play important roles in site remediation. The recently reported Dhgm sp. strain WBC-2 and 'Candidatus Dehalogenimonas etheniformans' strain GP are capable of dechlorinating certain chlorinated ethenes. More importantly, strain GP can completely detoxify the carcinogenic vinyl chloride (VC) to ethene. These findings expand the diversity of microorganisms involved in the respiratory VC reductive dechlorination and improve the understanding of Dhgm's ecological functions. Here, we summarize the advances in physiological and biochemical characteristics, ecological functions and genomic features of Dhgm, with the aim to develop effective and sustainable strategies to facilitate the bioremediation of chlorinated compounds contaminated sites.


Subject(s)
Anaerobiosis , Biodegradation, Environmental , Chloroflexi , Water Pollutants, Chemical
4.
Chinese Journal of Biotechnology ; (12): 3425-3438, 2021.
Article in Chinese | WPRIM | ID: wpr-921439

ABSTRACT

The facultative anaerobic and strict anaerobic microorganisms enriched and acclimated during the anaerobic digestion process are crucial for the efficiency of the anaerobic digestion system. Most of the problems encountered during running anaerobic digestion processes could be effectively improved via stimulation of microbial metabolic activity. Benefited from the rapid development of microbiome techniques, deeper insights into the microbial diversity in anaerobic digestion systems, e.g. the microbe-microbe interactions and microbe-environment interactions, have been gained. A complex and intricate metabolic network exists in the anaerobic digestion system of solid organic wastes. However, little is known about these interactions and the underlying mechanisms. This review briefly summarized the representative interactions between microbial communities during anaerobic digestion process discovered to date. In addition, typical issues encountered during the anaerobic digestion of solid organic wastes and how microbes can tackle and alleviate these issues were discussed. Finally, future priorities on microbiome research were proposed based on present contribution of microbiome analysis in anaerobic digestion system.


Subject(s)
Anaerobiosis , Bioreactors , Methane , Microbial Interactions , Microbiota , Solid Waste
5.
Chinese Journal of Biotechnology ; (12): 448-460, 2021.
Article in Chinese | WPRIM | ID: wpr-878574

ABSTRACT

Resource utilization is an effective way to cope with the rapid increase of kitchen waste and excess sludge, and volatile fatty acids produced by anaerobic fermentation is an important way of recycling organic waste. However, the single substrate limits the efficient production of volatile fatty acids. In recent years, volatile fatty acids produced by anaerobic co-fermentation using different substrates has been widely studied and applied. In this paper, we analyze the characteristics of fermentation to produce acid using kitchen waste and excess sludge alone or mixture. Influences of environmental factors and microbial community structure on the type and yield of volatile fatty acids in the anaerobic fermentation system are discussed in detail. Moreover, we propose future research directions, to provide a reference for recycling kitchen waste and excess sludge.


Subject(s)
Anaerobiosis , Bioreactors , Fatty Acids, Volatile , Fermentation , Hydrogen-Ion Concentration , Microbiota , Organic Chemicals , Sewage
6.
Chinese Journal of Biotechnology ; (12): 149-162, 2021.
Article in Chinese | WPRIM | ID: wpr-878550

ABSTRACT

Polyhydroxyalkanoates (PHA) synthesis by activated sludge using volatile fatty acids (VFAs) in fermentation liquid of excess sludge as carbon source is a hotspot in the field of environmental biotechnology. However, there is no unified conclusion on the effects of non-VFAs, mainly dissolved organic matter (DOM), on PHA production. Thus, this critical review mainly introduces the main characteristics and common analysis methods of DOM in anaerobic fermentation liquid. The effects of DOM on PHA production are analyzed from the aspects of microbiology, metabolic regulation and sludge properties. The results of different studies showed that high concentration of DOM is bad for PHA production, but an appropriate amount of DOM is conducive to the stability of sludge properties, reducing the final PHA purification cost. Finally, suitable strategies were proposed to regulate the PHA synthesis by activated sludge with DOM for PHA production by anaerobic fermentation liquid.


Subject(s)
Anaerobiosis , Bioreactors , Fatty Acids, Volatile , Fermentation , Polyhydroxyalkanoates , Sewage
7.
Chinese Journal of Biotechnology ; (12): 2824-2837, 2020.
Article in Chinese | WPRIM | ID: wpr-878532

ABSTRACT

In order to explore the microbial communities and functions of activated sludge in an Anaerobic-anoxic-oxic (A²/O) process under the start-up of Actinic reaction enzyme system (ARES) system and to understand the impact of the ARES system in domestic sewage treatment process, the activated sludge microbial community structure in the A²/O process system before and after ARES system start-up was analyzed by Illumina-HiSeq 2000 high-throughput sequencing platform. By combining with the main parameters related to the effect of sewage treatment, we analyzed the environmental functions of the microbial communities. The microbial community structure of activated sludge was significantly different before and after the ARES system start-up. There were 9 main bacterial phyla in the system (average relative abundance ≥1%), accounting for 96%-98% of the total bacteria sequenced. After the ARES system was started, the relative abundance of Betaproteobacteria and Chlorobi increased by 3.45%-3.85% and 0.45%-2.61%, respectively. In the anaerobic unit, the relative abundance of Bacteroidetes increased by 12.97%, while the Actinobacteria and Firmicutes decreased by 9.60% and 1.45%, respectively. At the genus level of bacteria, the relative abundance of Denitratisoma increased by 0.80%-3.27%, while the Haliangium and Arcobacter decreased by 3.36%-4.52% and 1.48%-3.45%, respectively. The relative abundance of bacteria was significantly different before and after the ARES system start-up. There were 7 abundant fungi phyla (average relative abundance ≥1%) in the system. After the ARES system was started, the relative abundance of Rozellomycota decreased by 42.71%-46.77%. In the anaerobic unit, the relative abundance of Ascomycota decreased by 13.39%, while the relative abundance of Glomeromycota increased by 13.86%. At the genus level of fungi. The relative abundance of Entomophthoraceae sp. and Glomcromycota sp. increased by 31.35%-36.50% and 6.27%-13.84%, respectively, while the Rozellomycota sp. and Xylochrysis lucida decreased by 42.71%-46.77% and 3.67%-5.54%, respectively. Our results showed that the application of ARES system caused the response of the microbial community to environmental changes, especially for the fungi communities, in the meanwhile, improved the effluent quality, especially the removal rate of total nitrogen.


Subject(s)
Anaerobiosis , Ascomycota , Bioreactors , Microbiota , Nitrogen , Sewage , Waste Disposal, Fluid
8.
Braz. arch. biol. technol ; 63: e20180130, 2020. graf
Article in English | LILACS | ID: biblio-1132195

ABSTRACT

Abstract Color removal from textile effluents was evaluated using a laboratory-combined process based on an upflow anaerobic sludge blanket (UASB) reactor followed by a shallow polishing pond (SPP). The anaerobic reactor was fed with a real textile effluent, diluted 10-times in a 350 mg/L solution of pre-treated residual yeast extract from a brewery industry as nutrient source. The parameters color, COD, N-NH3 and toxicity were monitored throughout 45 days of operation. According to the results, decolorization and COD removal were highest in the anaerobic step, whereas the effluent was polished in the SPP unit. The overall efficiency of the complete UASB-SPP system for COD and color were 88 and 62%, respectively. Moreover, the N-NH3 generated by the residual yeast extract ammonification was below 5 mg/L for the final effluent. Finally, no toxicity was detected after the treatment steps, as shown by the Vibrio fischeri microscale assay.


Subject(s)
Animals , Textiles/toxicity , Waste Disposal, Fluid/methods , Water Purification/methods , Yeasts , Toxicity Tests , Bioreactors , Aliivibrio fischeri , Anaerobiosis
9.
Electron. j. biotechnol ; 37: 25-33, Jan. 2019. tab, graf, ilus
Article in English | LILACS | ID: biblio-1051225

ABSTRACT

Background: Ammonium stress is a prime limiting phenomenon that occurs during methane formation from poultry manure. It is caused by elevated ammonium nitrogen concentrations that result from substrate decomposition. The amounts of methane formed depend on the activity of methanogenic microbes. Results: During the research reported in this paper, the response of a mesophilic consortium inhabiting a biogas reactor to rising load of poultry manure was investigated. The taxonomic composition of bacterial population was mostly typical, however syntrophic bacteria were not detected. This absence resulted in limitation of succession of some methanogenic microorganisms, especially obligate hydrogenotrophs. The methanogenic activity of the consortium was totally dependent on the activity of Methanosaeta. Inhibition of methanoganesis was noticed at ammonium nitrogen concentration of 3.68 g/L, total cessation occurred at 5.45 g/L. Significant amounts of acetic acid in the fermentation pulp accompanied the inhibition. Conclusions: The effectiveness of the consortium was totally dependent on the metabolic activity of the acetoclastic Methanoseata genus and lack of SAOB did not allow hydrogenotrophic methanogens to propagate and lead to cessation of biogas production at an elevated ammonium concentration at which acetoclastic methanogens were inhibited.


Subject(s)
Methanosarcinaceae/metabolism , Biofuels , Microbiota , Anaerobiosis , Poultry , Stress, Physiological , Polymerase Chain Reaction , DNA Fingerprinting , Chromatography, High Pressure Liquid , Archaea/metabolism , Biodiversity , Fermentation , Microbial Consortia , Ammonium Compounds , Manure , Methane , Nitrogen
10.
Electron. j. biotechnol ; 39: 61-66, may. 2019. graf, tab
Article in English | LILACS | ID: biblio-1052032

ABSTRACT

BACKGROUND: Triclosan (TCS) is an antimicrobial agent widely used in health care and consumer products. This compound is present in sludge of wastewater treatment plants (WWTPs), and because of its bactericidal characteristics, it can inhibit the methanogenic activity in anaerobic digestion (AD) technology. The aim of this study was to evaluate the toxic effects of TCS on the methanogenic activity. RESULTS: Batch anaerobic reactors were used with TCS concentrations of 7.8, 15.7, 23.5, and 31.4 mg/L. These assays consisted in three successive feedings (I, II, and III), wherein the sludge was exposed to each TCS concentration and volatile fatty acid (VFA) substrate. For evaluation of the residual sludge activity during feeding III, only VFA was used. The results showed that the increase in TCS concentrations correlated with the reduction in methane (CH4) production. In this case, the minimum values were achieved for TCS concentration of 31.4 mg/L with CH4 levels between 101.9 and 245.3 during feedings I, II, and III. Regarding the effect of TCS on VFA consumption, an inhibitory effect was detected for TCS concentrations of 23.5 and 31.4 mg/L, with concentrations of acetic, butyric, and propionic acids at the end of the assay (37 d) between 153.6 and 206.8, 62.5 and 60.1, and 93.4 and 110 mg/L, respectively. Regarding the removal of TCS during AD, these values were above 47%. Conclusion: TCS is an inhibitor of methanogenic activity with a decrease between 63 and 70% during the different feedings. The CH4 production was not recovered during feeding III, with inhibition percentages of 21­72%.


Subject(s)
Triclosan/toxicity , Anaerobic Digestion , Methane/metabolism , Anti-Infective Agents/toxicity , Sewage , Wastewater Treatment Plants , Chromatography, High Pressure Liquid , Fatty Acids, Volatile , Anaerobiosis
11.
Electron. j. biotechnol ; 39: 98-106, may. 2019. tab, graf, ilus
Article in English | LILACS | ID: biblio-1052269

ABSTRACT

BACKGROUND: The amount of municipal solid waste (MSW) gradually increased along with the rapid development of modern cities. A large amount of landfill leachate are generated with excessive chemical oxygen demand (COD), which create a great deal of pressure on the environment-friendly treatment process. Anaerobic digestion is an ideal technique to solve the above problem. RESULTS: A thermophilic granular sludge was successfully adapted for anaerobic digestion of MSW leachate (from an aging large-scale landfill) for methane production. The COD degradation efficiency improved by 81.8%, while the methane production rate reached 117.3 mL CH4/(g VS d), which was 2.34-fold more than the control condition. The bacterial and archaeal communities involved in the process were revealed by 16S rRNA gene high-throughput pyrosequencing. The richness of the bacterial community decreased in the process of thermophilic granular sludge, while the archaeal community structure presented a reverse phenomenon. The bacterial genus, Methanosaeta was the most abundant during the mesophilic process, while Methanobacterium, Methanoculleus, Methanosaeta and Methanosarcina were more evenly distributed. The more balanced community distribution between hydrogenotrophic and acetotrophic methanogens implied a closer interaction between the microbes, which further contributed to higher methane productivity. The detailed relationship between the key functional communities and anaerobic digestion performances were demonstrated via the multivariate canonical correspondence analysis. Conclusions: With the assistance of adaptive thermophilic granular sludge, microbial community structure was more evenly distributed, while both of COD degradation rate and methane production was improved during anaerobic digestion of MSW landfill leachate.


Subject(s)
Bacteria, Anaerobic/metabolism , Solid Waste , Anaerobic Digestion , Sludge Treatment , Methane/metabolism , Sewage/microbiology , Bacteria, Anaerobic/isolation & purification , Water Pollutants, Chemical , Polymerase Chain Reaction , Urban Area , Biofuels , Biological Oxygen Demand Analysis , Hot Temperature , Anaerobiosis
12.
Educ. fis. deporte ; 38(1): https://revistas.udea.edu.co/index.php/educacionfisicaydeporte/article/view/338652, Enero 2019.
Article in Spanish | LILACS | ID: biblio-1104417

ABSTRACT

Objetivo: determinar factores asociados a la potencia anaeróbica y capacidad cardiorrespiratoria en escolares de 12 a 17 años de Medellín-Colombia. Método: estudio transversal con escolares, a quienes se les realizaron mediciones sociodemográficas (sexo, edad, grado de escolaridad), antropométricas (peso, talla, circunferencia de cintura y de cadera, sobrepeso, obesidad abdominal), y de condición física (potencia anaeróbica, índice de fatiga y capacidad cardiorrespiratoria). Resultados: las variables antropométricas y sociodemográficas influyen en la condición física. Padecer sobrepeso, obesidad abdominal y ser mujer, fueron factores que se asociaron con un menor desarrollo en las pruebas de potencia anaeróbica y con la capacidad cardiorrespiratoria. La mediana de generación de watts de potencia por kilogramo en una persona con obesidad abdominal fue de 4, comparado con 6,9 en alguien sin esta condición. La potencia anaeróbica se correlaciona con la capacidad cardiorrespiratoria. Conclusiones: se logró identificar que sí hay asociación entre la potencia anaeróbica y algunas variables antropométricas y sociodemográficas.


Objective: To determine associated factors with anaerobic power and cardiorespiratory fitness in schoolchildren aged 12 to 17 years old in Medellín, Colombia. Methods: Cross-sectional study with schoolchildren, who underwent to sociodemographic (sex, age, level of education), anthropometric (weight, height, waist and hip circumference, overweight, abdominal obesity), and physical condition (anaerobic power, fatigue and cardiorespiratory fitness) measurements. Results: Anthropometric and sociodemographic variables influence the physical fitness. Being overweight, abdominal obesity and being a woman were factors associated with lesser development in anaerobic power tests and cardiorespiratory fitness. Median of watts of power per kilogram in a person with abdominal obesity was 4, compared to 6.9 in someone without this condition. Anaerobic power correlates with cardiorespiratory fitness. Conclusions: It was possible to identify that there is an association between anaerobic power and some anthropometric and sociodemographic variables.


Objetivo: determinar os fatores associados à potência anaeróbia e à capacidade cardiorrespiratória em escolares de 12 a 17 anos de Medellín-Colômbia. Métodos: estudo transversal em escolares em que foram desenvolvidas medições sociodemográficas (sexo, idade, grau de escolaridade), antropométricas (peso, estatura, circunferência da cintura e quadril, sobrepeso, obesidade abdominal) e de condição física (Potência anaeróbia, taxa de fadiga e capacidade cardiorrespiratória). Resultados: as variáveis antropométricas e sociodemográficas influenciam a condição física. O sobrepeso, a obesidade abdominal e ser mulher foram fatores que se associaram com menor desenvolvimento nos testes de potência anaeróbia e com a capacidade cardiorrespiratória. A geração mediana de watts de poder por quilograma em uma pessoa com obesidade abdominal era 4, comparada com o 6,9 em alguém sem esta circunstância. O poder anaeróbio se correlaciona com a capacidade cardiorrespiratória. Conclusões: identificou-se que se existe ligação entre potência anaeróbia e algumas variáveis antropométricas e sociodemográficas.


Subject(s)
Schools , Sports , Exercise , Cardiorespiratory Fitness , Anaerobiosis
13.
Article in English | WPRIM | ID: wpr-773367

ABSTRACT

OBJECTIVE@#To investigate the development and characterizations of the hepatocytes isolated from fetal ovine and to determine the effect of hypoxia on their growth and metabolism.@*METHODS@#Fresh hepatocytes were isolated from the liver of fetal ovine at late gestation, cultured in specific media, and exposed to normoxia (21% O2) or hypoxia (2% O2). The cellular characteristics and population purity were identified by immunocytochemistry and flow cytometry (FCM). The effects of hypoxia on cell cycle and apoptosis of the hepatocytes were evaluated by FCM, whereas the cellular ultrastructure changes were examined with a transmission electron microscope.@*RESULTS@#The cell purity of hepatocytes was over 95%. Under hypoxia exposure, the hepatocytes showed a gradual increase in proportion at the S phase and in proliferative index, followed with a compatible increase in apoptosis and progressively decreased cell viability. Additionally, the organelles of the hepatocytes demonstrated dramatic changes, including swelling of mitochondria, disorder in cristae arrangement, expansion of endoplasmic reticulum, and a large number of circular lipid droplets emerging in the cytoplasm.@*CONCLUSION@#Fetal ovine hepatocytes could be primarily cultured in a short-term culture system with a high purity of over 95% and with their preserved original characteristics. Hypoxia could induce changes in ultrastructural and inhibit the proliferation of cultured fetal ovine hepatocytes through apoptotic mechanisms.


Subject(s)
Anaerobiosis , Animals , Cell Culture Techniques , Fetus , Physiology , Hepatocytes , Physiology , Oxygen , Sheep , Physiology
14.
Braz. arch. biol. technol ; 62: e19180504, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055407

ABSTRACT

Abstract The aim of this work is to evaluate the performance of upflow anaerobic fixed bed reactors filled with espresso coffee capsules to treat sanitary sewage. Three reactors (R1, R2 and R3) were constructed in blue PVC pipes measuring 30 cm height and 150 mm diameter and filled with coffee capsules made of aluminum and plastic. The sewage from the pre-treatment phase of the wastewater treatment plant of the Federal University of Lavras fed the system. Temperature, pH, alkalinity and volatile acids concentration, COD, TS, TVS and TSS of the influent and effluent were analyzed to evaluate the reactors performances. Statistics tests were run in the software Statistica 10. Changes occurred in the organic loading rates caused two different operating phases, one at an OLR of 2.1 kg COD m-3d-1 and another at 4.0 kg COD m-3d-1. The average temperature during the monitoring period was 18°C. In spite of the operating conditions variations, the reactors showed satisfactory performances, presenting COD efficiency removals up to 80% in both phases. The capsules characteristics were similar to other materials used as support. Hence, it is possible to utilize coffee capsules as support material in anaerobic reactors, providing satisfactory pollutants removal efficiencies.


Subject(s)
Domestic Effluents , Biomass , Equipment Reuse , Efficiency , Anaerobiosis , Immobilization
15.
Motriz (Online) ; 25(3): e101910, 2019. tab, ilus
Article in English | LILACS | ID: biblio-1040651

ABSTRACT

Aim: The present study aimed to verify if there is a difference between genders in anaerobic capacity estimated by energetic equivalents of glycolytic and phosphagen pathways (AC[La-]+EPOCfast). Methods: In this way, 8 men and 8 women (physical education students) were subjected to the following sequence of tests: session 1) graded exercise test to measure the maximal oxygen consumption (VÖ½ O2max) and intensity associated with VÖ½ O2max (iVÖ½ O2max); sessions 2 to 3) familiarization with supramaximal effort at 115% of iVÖ½ O2max; session 4) supramaximal effort at 115% of iVÖ½ O2max to measure AC[La-]+EPOCfast. Results: The AC[La-]+EPOCfast was lower in women compared to men when expressed in absolute and relative values (-38.11%; p=0.01 and -25.71%; p=0.03, respectively). A non-significant difference was observed in performance in the supramaximal effort (-12.08%; p=0.15), besides which, a likely negative inference was observed when comparing women to men. In addition, energetic equivalents of the glycolytic pathway (e[La- ]) were also lower in women when expressed in relative and absolute values (-47.01%; p=0.001 and -36.71%; p=0.001, respectively), however no statistical difference was found for energetic equivalents of the phosphagen pathway (ePCr) (p>0.05). Conclusion: The AC[La-]+EPOCfast is lower in women compared to men, mainly due to differences in the glycolytic pathway.(AU)


Subject(s)
Humans , Male , Female , Adult , Sex Characteristics , Physical Exertion/physiology , Lactic Acid/blood , Glycolysis/physiology , Anaerobiosis/physiology
16.
Braz. j. microbiol ; 49(4): 770-776, Oct.-Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-974307

ABSTRACT

ABSTRACT Anaerobic digestion is important for the management of livestock manure with high ammonia level. Although ammonia effects on anaerobic digestion have been comprehensively studied, the molecular mechanism underlying ammonia inhibition still remains elusive. In this study, based on metatranscriptomic analysis, the transcriptional profile of microbial community in anaerobic digestion under low (1500 mg L-1) and high NH4 + (5000 mg L-1) concentrations, respectively, were revealed. The results showed that high NH4 + concentrations significantly inhibited methane production but facilitated the accumulations of volatile fatty acids. The expression of methanogenic pathway was significantly inhibited by high NH4 + concentration but most of the other pathways were not significantly affected. Furthermore, the expressions of methanogenic genes which encode acetyl-CoA decarbonylase and methyl-coenzyme M reductase were significantly inhibited by high NH4 + concentration. The inhibition of the co-expressions of the genes which encode acetyl-CoA decarbonylase was observed. Some genes involved in the pathways of aminoacyl-tRNA biosynthesis and ribosome were highly expressed under high NH4 + concentration. Consequently, the ammonia inhibition on anaerobic digestion mainly focused on methanogenic process by suppressing the expressions of genes which encode acetyl-CoA decarbonylase and methyl-coenzyme M reductase. This study improved the accuracy and depth of understanding ammonia inhibition on anaerobic digestion.


Subject(s)
Bacteria/genetics , Bacteria/metabolism , Ammonia/metabolism , Bacteria/isolation & purification , Bacteria/classification , Transcription, Genetic , Bioreactors/microbiology , Fatty Acids, Volatile/metabolism , Microbiota , Anaerobiosis , Methane/metabolism
17.
Prensa méd. argent ; 104(8): 391-402, oct2018. tab, fig
Article in Spanish | LILACS, BINACIS | ID: biblio-1050463

ABSTRACT

Objetivo: Determinar la Relación de la saturación central venosa de oxígeno (ScvO2) >_70% con la mortalidad, en el choque séptico en pacientes que ingresan al servicio de terapia intensiva pediátrica del HGR 36, Puebla. Métodos: Estudio, descriptivo, longitudinal, observacional. Se identificaron todos los pacientes de un mes a 14 años de edad que ingresaron a unidad de terapia intensiva con el diagnóstico de choque séptico. Se corroboró la colocación de un catéter venoso central para la medición de la ScvO2 a su ingreso y las 6 horas. Calificamos con el Indice Pediátrico de Mortalidad (PIM2) para medir el riesgo de mortalidad en cada paciente. Se realizó estadística descriptiva. Resultados: Fueron 15 pacientes, 8 (53.3%) femeninos y 7 (46.7%) masculinos. El PIM2 obtuvo un promedio de 7.42 % al ingreso, y a las 6 horas fue de 13.4%. El promedio de la saturación venosa central de oxígeno al ingreso de los pacientes a la terapia intensiva pediátrica fue de 56% y a las 6 horas el promedio alcanzó 71%. Ningún paciente falleció durante la reanimación cardiiopulmonar desde su ingreso. Conclusión: En base a los resultados anteriores podemos concluir, que no hay una correlación entre la ScvO2 >_ 70% y la mortalidad en los pacientes pediátricos con choque séptico


Objective: To determine the ratio of central venous oxygen saturation (ScvO2) >_ 70% mortality in septic shock patients admitted to pediatric intensive care unit of the HGR 36, Puebla. Methods: A descriptive, longitudinal, observational study. We identified all patients from one month to 14 years of age who were admitted to ICU with a diagnosis of septic shock. It confirmed the placement of a central venous atheter for the measurrement of income and ScvO2 to 6 hours. Qualified with the Pediatric Index of Mortality (PIM2) to measure the risk of death in each patient. We performed descriptive statistics. Results: there were 15 patients,eight (53.3%) female and 7 (46.7%) male. The PIM2 obtained an average of 7.42%. To entry, and 6 hours was 13.4%. The mean central venous oxygen saturation on admission of patients to the pediatric intensive care was 56% and 6 hours on average reached 71%. No patient died during cardiopulmonary resuscitation from your income. Conclusion: Based on previous results we can conclude that there is no coelation between ScvO2 >_70% and mortality in pediatric patients with septic shock


Subject(s)
Humans , Infant, Newborn , Infant , Child, Preschool , Adolescent , Oxygen Consumption , Shock, Septic/mortality , Biomarkers , Systemic Inflammatory Response Syndrome/drug therapy , Sepsis/mortality , Critical Care , Anaerobiosis , Hypoxia/diagnosis
19.
Metro cienc ; 26(1): 39-42, jun. 2018.
Article in Spanish | LILACS | ID: biblio-981567

ABSTRACT

Cuando hablamos del aclaramiento de lactato debemos conocer con claridad la complejidad del proceso, pues abarca todo el metabolismo del lactato desde su producción hasta su remoción: a este proceso se ha denominado equilibrio reversible de lactato. Este concepto que permitirá entender mejor los procesos dinámicos de su metabolismo no como producto final o de desecho (según, tradicionalmente, se nos ha hecho creer) sino, más bien, como un producto intermedio que ejerce funciones específicas y bien definidas que lo convierten no sólo en indicador de perfusión tisular sino en un indicador global del metabolismo celular.


When we talk about lactate clearance we have to be clear about the complexity of the process, since it involves the metabolism of lactate from its production to its removal what has been called reversible lactate equilibrium, this concept that will allow us to better understand the dynamic processes of its metabolism not as a final product or waste, as we have traditionally been led to believe, but rather as an intermediate product with specific and welldefined functions that make it not only an indicator of tissue perfusion, but a global indicator of metabolism cell


Subject(s)
Humans , Acid-Base Equilibrium , Metabolic Clearance Rate , Lactic Acid , Anaerobiosis , Energy Metabolism
20.
Braz. j. microbiol ; 49(2): 258-268, Apr.-June 2018. graf
Article in English | LILACS | ID: biblio-889233

ABSTRACT

Abstract Cellulosimicrobium cellulans CWS2, a novel strain capable of utilizing benzo(a)pyrene (BaP) as the sole carbon and energy source under nitrate-reducing conditions, was isolated from PAH-contaminated soil. Temperature and pH significantly affected BaP biodegradation, and the strain exhibited enhanced biodegradation ability at temperatures above 30 °C and between pH 7 and 10. The highest BaP removal rate (78.8%) was observed in 13 days when the initial BaP concentration was 10 mg/L, and the strain degraded BaP at constant rate even at a higher concentration (50 mg/L). Metal exposure experimental results illustrated that Cd(II) was the only metal ion that significantly inhibited biodegradation of BaP. The addition of 0.5 and 1.0 g/L glucose enhanced BaP biodegradation, while the addition of low-molecular-weight organic acids with stronger acidity reduced BaP removal rates during co-metabolic biodegradation. The addition of phenanthrene and pyrene, which were degraded to some extent by the strain, showed no distinct effect on BaP biodegradation. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that the five rings of BaP opened, producing compounds with one to four rings which were more bioavailable. Thus, the strain exhibited strong BaP degradation capability and has great potential in the remediation of BaP-/PAH-contaminated environments.


Subject(s)
Soil Microbiology , Soil Pollutants/metabolism , Benzo(a)pyrene/metabolism , Actinobacteria/isolation & purification , Actinobacteria/metabolism , Temperature , Cadmium/metabolism , Carbon/metabolism , Carboxylic Acids/metabolism , Biotransformation , Actinobacteria/classification , Culture Media/chemistry , Enzyme Inhibitors/metabolism , Glucose/metabolism , Hydrogen-Ion Concentration , Anaerobiosis , Gas Chromatography-Mass Spectrometry
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