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Braz. j. microbiol ; 42(1): 114-125, Jan.-Mar. 2011. ilus
Article in English | LILACS | ID: lil-571382


The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. After 6 hours of inoculation, H. seropedicae was already adhered to the roots, colonizing mainly root hair surface and bases, followed by epidermal cell wall junctions. Bacteria adherence in the initial periods occurred mainly in the form of solitary cells and small aggregates with pleomorphic cells. Bacteria infection of root tissue occurred through the cavities caused by the disruption of epidermal cells during the emergence of lateral roots and the endophytic establishment by the colonization of intercellular spaces of the cortical parenchyma. Moreover, within 1 day after inoculation the bacteria were colonizing the shoots. In this region, the preferred sites of epiphytic colonization were epidermal cell wall junctions, peltate scutiform trichomes and non-glandular trichomes. Subsequently, the bacteria occupied the outer periclinal walls of epidermal cells and stomata. The penetration into the shoot occurred passively through stoma aperture followed by the endophytic establishment on the substomatal chambers and spread to the intercellular spaces of spongy chlorenchyma. After 21 days of inoculation, bacterial biofilm were seen at the root hair base and on epidermal cell wall surface of root and leaf, also confirming the epiphytic nature of H. seropedicae.

Ananas/growth & development , Ananas/genetics , Biofilms , Cell Wall , Nitrogen Fixation/genetics , Herbaspirillum/growth & development , Herbaspirillum/isolation & purification , In Vitro Techniques , Microscopy, Electron , Green Fluorescent Proteins/analysis , Genetic Techniques , Methods , Microscopy, Fluorescence , Plants
Braz. j. microbiol ; 42(1): 203-210, Jan.-Mar. 2011. ilus, tab
Article in English | LILACS | ID: lil-571390


The study of thermal resistant mould, including Byssochlamys nivea, is of extreme importance since it has been associated with fruit and fruit products. The aim of this work is to analyze the influence of water activity (a w) and ascospore age (I) on the growth of Byssochlamys nivea in pineapple juice. Mold growth was carried out under different conditions of water activity (a w) (0.99, 0.96, 0.95, 0.93, 0.90) and ascospore age (I) (30, 51, 60, 69, 90 days). Growth parameters as length of adaptation phase (λ), maximum specific growth rate (µmax) and maximum diameter reached by the colony (λ) were obtained through the fit of the Modified Gompertz model to experimental data (measuring radial colony diameter). Statistica 6.0 was used for statistical analyses (significance level α = 0.05). The results obtained clearly showed that water activity is statistically significant and that it influences all growth parameters, while ascospore age does not have any statistically significant influence on growth parameters. Also, these data showed that by increasing a w from 0.90 to 0.99, the λ value substantially decreased, while µmax and λ values rose. The data contributed for the understanding of the behavior of B. nivea in pineapple juice. Therefore, it provided mathematical models that can well predict growth parameters, also helping on microbiological control and products' shelf life determination.

Water/analysis , Ananas/growth & development , Ascomycota/growth & development , Fungi/growth & development , Models, Theoretical , Pest Control, Biological , Spores, Fungal , Methods , Reference Standards , Carbonated Beverages , Methods
Ciênc. rural ; 36(1): 93-98, jan.-fev. 2006. tab, graf
Article in Portuguese | LILACS | ID: lil-419883


Apesar de ser uma planta com necessidades hídricas relativamente baixas, o abacaxizeiro tem demanda permanente de água, variável ao longo do ciclo e dependente do seu estádio de desenvolvimento. Assim, objetivou-se analisar volumes de irrigacão no desenvolvimento vegetativo, no rendimento da fruta e na otimizacão do abacaxizeiro cv. Pérola. O experimento foi realizado na Universidade Federal de Sergipe, município de São Cristóvão (11º01'S, 37º12'W), no delineamento em blocos ao acaso, com quatro tratamentos (lâminas de água) (100 por cento da evaporacão do tanque Classe A (523,7mm ano-1); 75 por cento da evaporacão do tanque Classe A (392,8mm ano-1), 50 por cento da evaporacão do tanque Classe A (261,8mm ano-1) e 0 por cento da evaporacão do tanque Classe A, seis repeticões e 12 plantas úteis por parcela. O sistema de irrigacão foi por aspersão convencional disposto em linha, com pressão de 20mca e vazão de 1,33m3 h-1. A área foliar (cmy) mínima atingida de 4552,6cmy foi observada no volume de água de 122,9mm ano-1, enquanto a massa seca das folhas (147,6g) foi constatada com 17mm ano-1. O máximo comprimento da folha D (88,9cm) foi estimado com 532,7 mm ano-1. Já o máximo comprimento do fruto (23cm) foi observado na lâmina de 296,9mm ano-1. A massa do fruto máxima estimado de 1.736g foi constatado na lâmina de 356,4mm ano-1. No contexto, a irrigacão contribui de forma positiva no desenvolvimento vegetativo e rendimento da fruta do abacaxizeiro. Ressalta-se remuneracão mensal líquida de R$ 1.161,17ha-1, quando se adota lâmina de irrigacão de 356,4mm ano-1.

Ananas/growth & development , Process Optimization , Therapeutic Irrigation