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1.
Chinese Journal of Hematology ; (12): 316-322, 2022.
Article in Chinese | WPRIM | ID: wpr-935088

ABSTRACT

Objective: To analyze the value of (11)C-PiB PET/MRI for evaluating organ involvement in patients with primary light chain amyloidosis (pAL) . Methods: The clinical data of 20 patients with pAL and 3 healthy volunteers from January 2019 to October 2021 were retrospectively analyzed. The correlation between the organ involvement evaluated by clinical standards and PET/MRI was compared. The relationship between cardiac-related biological indicators, disease stage, and the maximum standardized uptake value (SUVmax) were analyzed. The relationship between 24-hour urinary protein quantification and kidney SUVmax was analyzed. Results: ①In 20 patients (18 newly diagnosed patients and 2 non-newly diagnosed patients) ,(11)C-PiB positive uptake was observed in the heart (15 patients, 75%) , lung (8 patients, 40%) , bone marrow (10 patients, 50%) , muscle (10 patients, 50%) , tongue muscle (7 patients, 35%) , thyroid (6 patients, 30%) , salivary gland (4 patients, 20%) , spleen (2 patients, 10%) , and stomach wall (1 patient, 5%) . ②Organ involvement on (11)C-PiB PET/MRI showed good correlations with the clinical evaluation criteria for the heart and bone marrow. The positive rate of PET/MRI evaluation in the lung, spleen, gland, muscle, and tongue muscle was significantly higher than the clinical criteria. However, (11)C-PiB PET/MRI has limitations in the evaluation of the nervous system and fat tissue. ③To analyze the relationship between cardiac-related biological indexes and the SUVmax of the heart in 13 newly diagnosed patients. Patients with left ventricular ejection fraction (LVEF) <50% and interventricular septal thickness (ISV) ≥1.2 cm showed a higher SUVmax than patients with LVEF ≥50% and ISV<1.2 cm (P<0.05) .There are significant differences in the SUVmax of the heart between the Mayo2004 stage and the Mayo2012 stage. The later the disease stage, the higher the SUVmax (P<0.05) . The SUVmax of the heart was positively correlated with cardiac troponin I (cTnI) and N-terminal pro-brain natriuretic peptide (NT-proBNP) (P<0.01) .There was no significant correlation between renal SUVmax and 24-hour urine protein (P>0.05) . Conclusion: Whole body (11)C-PiB PET/MRI, as a visualization system of amyloid protein, is used to qualitatively evaluate organ involvement, which can improve the level of early non-invasive diagnosis. Whole body (11)C-PiB PET/MRI can be used to perform quantitative evaluation of organ levels, especially the heart, which is expected to evaluate organ function and predict disease prognosis more accurately.


Subject(s)
Humans , Amyloidosis/diagnostic imaging , Aniline Compounds , Magnetic Resonance Imaging , Positron-Emission Tomography , Retrospective Studies , Stroke Volume , Ventricular Function, Left
2.
Braz. J. Psychiatry (São Paulo, 1999, Impr.) ; 43(5): 510-513, Sept.-Oct. 2021. tab
Article in English | LILACS | ID: biblio-1345479

ABSTRACT

Objective: People with Alzheimer's disease (AD) dementia have impaired sleep. However, the characteristics of sleep in the early stages of AD are not well known, and studies with the aid of biomarkers are lacking. We assessed the subjective sleep characteristics of non-demented older adults and compared their amyloid profiles. Methods: We enrolled 30 participants aged ≥ 60 years, with no dementia or major clinical and psychiatric diseases. They underwent [11C]PiB-PET-CT, neuropsychological evaluations, and completed two standardized sleep assessments (Pittsburgh Sleep Quality Inventory and Epworth Sleep Scale). Results: Comparative analysis of subjective sleep parameters across the two groups showed longer times in bed (p = 0.024) and reduced sleep efficiency (p = 0.05) in individuals with positive amyloid. No differences in other subjective sleep parameters were observed. We also found that people with multiple-domain mild cognitive impairment (MCI) had shorter self-reported total sleep times (p = 0.034) and worse overall sleep quality (p = 0.027) compared to those with single-domain MCI. Conclusions: Older adults testing positive for amyloid had a longer time in bed and lower sleep efficiency, regardless of cognitive status. In parallel, individuals with multiple-domain MCI reported shorter sleep duration and lower overall sleep quality.


Subject(s)
Humans , Aged , Alzheimer Disease/diagnostic imaging , Cognitive Dysfunction/diagnostic imaging , Sleep , Thiazoles , Case-Control Studies , Positron-Emission Tomography , Positron Emission Tomography Computed Tomography , Aniline Compounds
3.
Chinese Journal of Lung Cancer ; (12): 804-807, 2021.
Article in Chinese | WPRIM | ID: wpr-922150

ABSTRACT

Osimertinib-induced interstitial lung disease (ILD) is an uncommon, but fatal pulmonary toxicity in some patients. We report a case of a 64-year-old male with stage IV adeno-non-small cell lung cancer (NSCLC) harboring an exon 19 deletion in the epidermal growth factor receptor (EGFR) treated with osimertinib 80 mg/d for first-line targeted therapy. On day 60 after initiating treatment of osimertinib, the patient developed ILD. Osimertinib was discontinued immediately and oral prednisone 60 mg/d was initiated, ILD improved within 13 d. After balancing the risk and benefit, osimertinib was restarted concurrently with prednisone. The patient showed neither disease progression nor a recurrence of ILD for more than 16 months. Based on our case and literature review, retreatment with osimertinib under steroid coverage could be considered as an effective treatment option after careful risk-benefit assessment for patients with EGFR-mutant NSCLC.
.


Subject(s)
Humans , Male , Middle Aged , Acrylamides/therapeutic use , Aniline Compounds/therapeutic use , Carcinoma, Non-Small-Cell Lung/genetics , ErbB Receptors/genetics , Lung Diseases, Interstitial/genetics , Lung Neoplasms/genetics , Prednisone , Protein Kinase Inhibitors/adverse effects
4.
Article in Chinese | WPRIM | ID: wpr-829052

ABSTRACT

OBJECTIVE@#To study the effect of apoptotic drug Navitoclax (NTX) combined with chemotherapy drug Daunorubicin (DNR) on apoptosis of erythroleukemia cells.@*METHODS@#K562, HEL and TF-1 cells in logarithmic growth phase were treated with NTX, DNR and combination of the two drugs. CCK-8 test, Annexin V-DAPI double-staining flow cytometry, real-time RT-PCR were used to detect cell growth, cell apoptosis and expression of BAX, BAK, BCL-2, BCL-xl and BIM respectively. The effects of NTX, DNR and combination of the two drugs on apoptosis of K562, HEL and TF-1 cells were compared and analyzed.@*RESULTS@#NTX combined with DNR could significantly inhibit the growth of K562, HEL and TF-1 cells; Apoptosis detection results showed that the apoptotic rate of K562, HEL and TF-1 cells in combination group was significantly higher than that in NTX and DNR single group; the expression level of apoptosis-related genes BAK and BAX in K562 cells in combination group was significantly higher than that in two single drug groups, and the expression level of anti-apoptotic protein genes BCL-2 and BCL-xl was significantly lower than that in two single drug groups (P<0.05); the expression level of BAK in HEL cells treated with combined drugs for 24 hours was higher than that in DNR group (P < 0.05); the expression level of BCL-2 in TF-1 cells treated with combined drugs for 24 hours was lower than that in two single drugs groups while the expression level of BAK in 48 hours was the highest in combined drugs group, and the expression level of BCL-2 and BCL-xl in combined drugs group was lower than that in NTX group (P<0.05).@*CONCLUSION@#NTX combined with DNR can significantly promote the apoptosis of erythroleukemia cell lines K562, HEL and TF-1, and induce the expression of apoptosis-related genes. This study provides a new scheme for the clinical treatment of erythroleukemia.


Subject(s)
Humans , Aniline Compounds , Apoptosis , Daunorubicin , K562 Cells , Leukemia, Erythroblastic, Acute , Sulfonamides
5.
Article in English | WPRIM | ID: wpr-1008980

ABSTRACT

A 61-year-old Chinese woman was diagnosed as primary pulmonary adenocarcinoma of left superior lobe with epidermal growth factor receptor (EGFR) 19 del mutation positive. Treatment with icotinib was given, but her disease progressed after 6 months remission. CT-guide needle biopsy for the new lesion in inferior lobe of left lung demonstrated intrapulmonary metastasis, and EGFR gene panel by Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS-PCR) confirmed EGFR T790M mutation. Treatment with osimertinib was initiated. After 2 months remission, the disease progressed. Re-biopsy was performed for the tumor in the inferior lobe of left lung, and ARMS-PCR demonstrated no other gene mutation except EGFR 19 del. Icotinib was re-challenged, but disease progressed continuously. Bevacizumab was added, and partial response was achieved after 2-cycle of combination therapy. The non-small cell lung cancer (NSCLC) in this case maintained EGFR activating mutation and lost EGFR T790M mutation was a genetic change after osimertinib treatment. This case suggests the re-challenge of the first-generation EGFR-TKIs combined with bevacizumab may overcome the tumor resistance and prolong survival of NSCLC patient.


Subject(s)
Female , Humans , Middle Aged , Acrylamides/therapeutic use , Adenocarcinoma of Lung/pathology , Aniline Compounds/therapeutic use , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Bevacizumab/therapeutic use , Carcinoma, Non-Small-Cell Lung/pathology , Cell Line, Tumor , Crown Ethers/therapeutic use , Drug Resistance, Neoplasm/drug effects , Lung Neoplasms/pathology , Quinazolines/therapeutic use , Tomography, X-Ray Computed , Treatment Outcome
6.
Rev. clín. periodoncia implantol. rehabil. oral (Impr.) ; 10(3): 145-148, dic. 2017. tab, graf
Article in English | LILACS | ID: biblio-900296

ABSTRACT

ABSTRACT: Aim: The objective of this work was to determine the concentrations of irrigating solutions and the residual content of parachloroaniline (PCA) formed after endodontic irrigation, using 5% NaOCl, 0.9% NaCl, 10% EDTA and 2% CHX 2%. Methodology Twenty premolars were used and 13 samples were collected per tooth from each of the treatment phases. Samples of: NaOCl, EDTA, CHX and PCA were quantified by UV and visible spectrophotometry. Results: Sodium hypochlorite decreased its concentration from 3.8% to 3.4% in phases 1 to 4. In phases 5, 6 and 7, residual NaOCl was measured with concentrations of 0.007%, 0.003% and 0.001% %. The concentration of EDTA decreased to 8.85% in phase 8. In phases 9, 10 and 11, irrigated with serum, EDTA was quantified with concentrations of 0.013% to 0.002% and NaOCl values of 0.0011% to 0, 0006%. In phases 12 and 13, CHX concentrations were 1.850% and 1.812% and PCA values were 0.0005% and 0.0007%. PCA formation occurred in presence of 2% CHX and residual NaClO and was detected colorimetrically in phases 12 and 13. Conclusions. During endodontic irrigation the concentration of 5% NaOCl decreases significantly in the first four phases and the concentrations of EDTA and CHX also decrease. There is PCA training in the last stages of the procedure.


Subject(s)
Humans , Root Canal Irrigants/chemistry , Sodium Hypochlorite/chemistry , Chlorhexidine/chemistry , Aniline Compounds/analysis , Root Canal Irrigants/administration & dosage , Sodium Hypochlorite/administration & dosage , Spectrophotometry , Chlorhexidine/administration & dosage , Edetic Acid/chemistry , Drug Interactions , Endodontics
7.
National Journal of Andrology ; (12): 195-199, 2017.
Article in Chinese | WPRIM | ID: wpr-812787

ABSTRACT

Advanced prostate cancer, especially at the castration-resistant stage, remains incurable clinically and, therefore, urgently requires new therapeutics for the patients. PI3K is a family of critical cell signal transduction molecules and their over-activation is an important factor in cancer development and progression. It has been demonstrated that class IA PI3K p110 is drastically overexpressed in prostate cancer and involved in androgen receptor-mediated gene expression and castration-resistant progression and regarded as a potential therapeutic target for prostate cancer. Several p110-specific inhibitors have been reported recently and two of them, GSK2636771 and AZD8186, are being tested in clinical trials.


Subject(s)
Humans , Male , Aniline Compounds , Therapeutic Uses , Chromones , Therapeutic Uses , Imidazoles , Therapeutic Uses , Morpholines , Therapeutic Uses , Neoplasm Proteins , Phosphatidylinositol 3-Kinases , Metabolism , Phosphoinositide-3 Kinase Inhibitors , Prostatic Neoplasms, Castration-Resistant , Drug Therapy , Protein Kinase Inhibitors , Therapeutic Uses
8.
Biol. Res ; 50: 8, 2017. graf
Article in English | LILACS | ID: biblio-838960

ABSTRACT

BACKGROUND: CD4+ T cells play an important role in the initiation of an immune response by providing help to other cells. Among the helper T subsets, interferon-γ (IFN-γ)-secreting T helper 1 (Th1) and IL-17-secreting T helper 17 (Th17) cells are indispensable for clearance of intracellular as well as extracellular pathogens. However, Th1 and Th17 cells are also associated with pathogenesis and contribute to the progression of multiple inflammatory conditions and autoimmune diseases. RESULTS: In the current study, we found that BJ-1108, a 6-aminopyridin-3-ol analogue, significantly inhibited Th1 and Th17 differentiation in vitro in a concentration-dependent manner, with no effect on proliferation or apoptosis of activated T cells. Moreover, BJ-1108 inhibited differentiation of Th1 and Th17 cells in ovalbumin (OVA)-specific OT II mice. A complete Freund's adjuvant (CFA)/OVA-induced inflammatory model revealed that BJ-1108 can reduce generation of proinflammatory Th1 and Th17 cells. Furthermore, in vivo studies showed that BJ-1108 delayed onset of disease and suppressed experimental autoimmune encephalomyelitis (EAE) disease progression by inhibiting differentiation of Th1 and Th17 cells. CONCLUSIONS: BJ-1108 treatment ameliorates inflammation and EAE by inhibiting Th1 and Th17 cells differentiation. Our findings suggest that BJ-1108 is a promising novel therapeutic agent for the treatment of inflammation and autoimmune disease.


Subject(s)
Animals , Female , Mice , Cell Differentiation/drug effects , Th1 Cells/drug effects , Encephalomyelitis, Autoimmune, Experimental/drug therapy , Th17 Cells/drug effects , Aminopyridines/pharmacology , Aniline Compounds/pharmacology , Spleen/immunology , CD4-Positive T-Lymphocytes/drug effects , CD4-Positive T-Lymphocytes/immunology , Reproducibility of Results , Apoptosis/drug effects , Apoptosis/immunology , Th1 Cells/immunology , Cell Proliferation/drug effects , Disease Models, Animal , Encephalomyelitis, Autoimmune, Experimental/immunology , Th17 Cells/immunology , Flow Cytometry , Aminopyridines/immunology , Aniline Compounds/immunology , Lymph Nodes/immunology , Mice, Inbred C57BL
9.
Article in English | WPRIM | ID: wpr-225578

ABSTRACT

This study aimed to investigate the independent and interactive influences of apolipoprotein E (APOE) epsilon4 and beta-amyloid (Abeta) on multiple cognitive domains in a large group of cognitively normal (CN) individuals and patients with mild cognitive impairment (MCI) and Alzheimer's disease (AD). Participants were included if clinical and cognitive assessments, amyloid imaging, and APOE genotype were all available from the Alzheimer's Disease Neuroimaging Initiative database (CN = 324, MCI = 502, AD = 182). Individuals with one or two copies of epsilon4 were designated as APOE epsilon4 carriers (epsilon4+); individuals with no epsilon4 were designated as APOE epsilon4 non-carriers (epsilon4-). Based on mean florbetapir standard uptake value ratios, participants were classified as Abeta burden-positive (Abeta+) or Abeta burden-negative (Abeta-). In MCI, APOE epsilon4 effects were predominantly observed on frontal executive function, with epsilon4+ participants exhibiting poorer performances; Abeta positivity had no influence on this effect. Abeta effects were observed on global cognition, memory, and visuospatial ability, with Abeta+ participants exhibiting poorer performances. Measures of frontal executive function were not influenced by Abeta. Interactive effects of APOE epsilon4+ and Abeta were observed on global cognition and verbal recognition memory. Abeta, not APOE epsilon4+, influenced clinical severity and functional status. The influences of APOE epsilon4+ and Abeta on cognitive function were minimal in CN and AD. In conclusion, we provide further evidence of both independent and interactive influences of APOE epsilon4+ and Abeta on cognitive function in MCI, with APOE epsilon4+ and Abeta showing dissociable effects on executive and non-executive functions, respectively.


Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Alzheimer Disease/genetics , Amyloid beta-Peptides/metabolism , Aniline Compounds/chemistry , Apolipoprotein E4/genetics , Brain/diagnostic imaging , Cognition , Databases, Factual , Demography , Ethylene Glycols/chemistry , Genotype , Cognitive Dysfunction/genetics , Positron-Emission Tomography
10.
Article in Spanish | LILACS | ID: lil-771673

ABSTRACT

Introducción: La clorhexidina (CHX) en solución acuosa se hidroliza generando paracloroanilina (PCA), proceso acelerado por el aumento de temperatura y pH. El uso de ultrasonido endodóntico (USE), basado en fenómenos de oscilación, cavitación, microcorriente acústica, genera calor afectando la CHX. Objetivo Identificar y cuantificar in vitro cambios fisicoquímicos, temperatura y pH, y la cantidad de PCA formada con el uso de ultrasonido endodóntico sobre soluciones de CHX al 2 por ciento. Materiales y métodos La CHX 2 por ciento se activó durante 30, 60, 90 y 120 seg con USE a 24.500 Hz, midiendo antes y después de activación: pH, temperatura y cantidad de PCA, leyendo a 375 nm en curva de calibración de estándares de PCA y formación, registrando el espectro de absorción mediante espectrofotómetro UV-visible. Resultados El USE aumentó la temperatura en 1 °C independiente del tiempo de aplicación y acidificó la solución de CHX 2 por ciento, sin variaciones significativas en pH y temperatura. No se observó coloración ni formación de precipitado en muestras activadas por USE a los diferentes tiempos. Las muestras no presentaron valores medibles de PCA a 375 nm. Los espectros de absorción de CHX 2 por ciento y activadas por USE por más de 60 seg presentaron curvas espectrales, peaks y valores de absorbancia diferentes. Conclusiones El USE aumenta la temperatura y acidifica la solución de la CHX en todos los tiempos de aplicación. No se detectó presencia de PCA por espectrofotometría visible. Muestras activadas por USE por 60 seg o más presentan espectros de absorción diferentes a las muestras sin activar. Diferencias en los espectros de absorción entre CHX activadas con USE y con CHX sin activar indicarían degradación de la CHX y posible presencia de PCA.


Introduction: Chlorhexidine (CHX) in aqueous solution is hydrolysed to p-chloroaniline (PCA), a process accelerated by increasing temperature and pH. Using endodontic ultrasound based on oscillation phenomena, cavitation, and acoustic microstreaming generates heat, affecting the CHX. Objective The aim of this in vitro study was to identify and quantify the physical-chemical changes, temperature and pH, and the amount of PCA formed by increasing the temperature of 2 percent CHX by endodontic ultrasound. Materials and methods Samples of 2 percent CHX were activated for 30, 60, 90, and 120 seconds with endodontic ultrasound 24,500 Hz. The pH and temperature were measured before and after activation, as well as the formation and amount of PCA, by reading and recording the result obtained from a standard calibration curve reading at 375 nm in a UV-visible light spectrophotometer. Results Independent of time, ultrasound increased the temperature of 2 percent CHX by 1 °C and acidified the solution. No significant changes were recorded in pH and temperature. No staining or precipitates were observed in samples ultrasonically activated at different times. Samples read at 375 nm showed no measurable PCA values. Absorption spectra of 2 percent CHX and 2 percent CHX activated for more than 60 seconds showed different spectral curves, peaks, and absorbance values. Conclusions Ultrasound increased the temperature and acidified the solution of CHX for all application times. No PCA was detected by visible spectrophotometry. Absorption spectra of 2 percent CHX activated with ultrasound at different times differs from 2 percent CHX without activation. These differences indicate degradation of CHX and possible presence of PCA.


Subject(s)
Chlorhexidine/chemistry , Aniline Compounds/chemistry , Ultrasonics , Disinfectants/chemistry , Hydrogen-Ion Concentration , In Vitro Techniques , Spectrophotometry , Temperature
11.
Int. j. odontostomatol. (Print) ; 9(3): 399-404, dic. 2015. ilus
Article in English | LILACS | ID: lil-775463

ABSTRACT

This study determined if p-chloroaniline (PCA) can be minimized by using distilled water and physiological saline solution following sodium hypochlorite but before chlorhexidine. Hypochlorite 5%, 0.5%, 0.05%, 0.005% and 0.0005% dissolved in 0.9% NaCl and in distilled water were mixed with 2% chlorhexidine for the formation of PCA. The PCA was determined using UV-VISIBLE spectrometry, with spectral curve was determined the wavelength of maximum absorption of PCA. Formed PCA absorbance was measured between 0.025%, 0.02%, 0.015%, 0.01%, 0.005% and 0.0025% hypochlorite and 2% chlorhexidine. 2% chlorhexidine and hypochlorite with physiological saline form a white precipitate which prevents the measurement of PCA. Colored PCA is formed with 0.05%, 0.005% hypochlorite aqueous dilutions and 2% chlorhexidine. The lwavelength of maximum absorption obtained was 470 nm and absorbance of PCA showed a linear decrease. 0.005% NaClO produces the least amount of PCA. The best solvent to prevent the formation of PCA during the interaction of sodium hypochlorite with chlorhexidine is distilled water.


Este estudio determinó si la p-cloroanilina (PCA) puede ser minimizada mediante el uso de agua destilada y solución salina fisiológica seguido de la aplicación de hipoclorito de sodio, previo a la aplicación de clorhexidina. Hipoclorito al 5%, 0,5%, 0,05%, 0,005% y 0,0005% fue disuelto en 0,9% de NaCl y en agua destilada se mezcló con 2% de clorhexidina para la formación de PCA. El PCA se determinó mediante espectrometría UV-Visible, y con curva espectral se determinó la longitud de onda máxima del PCA. La absorbancia del PCA formado se midió con 0,025%, 0,02%, 0,015%, 0,01%, 0,005% y 0,0025% de hipoclorito y 2% de clorhexidina. La combinación de 2% de clorhexidina e hipoclorito en solución salina fisiológica forman un precipitado blanco que impide la medición del PCA. El PCA coloreado es formado con 0,05%, 0,005% diluciones acuosas de hipoclorito y 2% de clorhexidina. La longitud de onda máxima obtenida fue de 470 nm y la absorbancia del PCA mostró una disminución lineal. NaClO al 0,005% produce menor cantidad de PCA. El mejor disolvente para evitar la formación de PCA durante la interacción de hipoclorito de sodio con clorhexidina es agua destilada.


Subject(s)
Acrylates/toxicity , Aniline Compounds/toxicity , Sodium Hypochlorite/therapeutic use , Distilled Water , Saline Solution/therapeutic use
12.
Braz. j. microbiol ; 46(1): 103-111, 05/2015. graf
Article in English | LILACS | ID: lil-748233

ABSTRACT

Degradation of 2,4,6-trinitrotoluene (TNT), a nitroaromatic explosive found in the soil and ground water, was investigated using Pseudomonas aeruginosa in in vitro experiments. Biodegradable abilitiy of this bacteria was performed with 50 and 75 mg L−1 TNT concentrations in a defined liquid medium for 96 h time period. Treatment of TNT in supernatant samples taken at 0, 6, 12, 24, 48, 72 and 96 h from agitated vessels was followed by reverse-phase high-performance liquid chromatography (HPLC). In cultures supplemented with 50 and 75 mgL−1 TNT, after 96 h of incubation 46% and 59% reduction were detected respectively. Two metabolites as degradation intermediates with nitrite release into the medium, 2,4-dinitrotoluene (2,4-DNT) and 4-aminodinitrotoluene (4-ADNT), were elucidated by thin layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). These findings clearly indicate that Pseudomonas aeruginosa can be used in bioremediation of TNT contaminated sites.


Subject(s)
Metabolic Networks and Pathways , Pseudomonas aeruginosa/metabolism , Trinitrotoluene/metabolism , Aniline Compounds/analysis , Biotransformation , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Culture Media , Dinitrobenzenes/analysis , Gas Chromatography-Mass Spectrometry , Time Factors
13.
J. appl. oral sci ; 23(2): 224-229, Mar-Apr/2015. graf
Article in English | LILACS, BBO | ID: lil-746547

ABSTRACT

A 43-year-old woman with a unilateral cleft lip and palate, presenting a totally edentulous maxilla and mandible with marked maxillomandibular discrepancy, attended the Prosthodontics section of the Hospital for Rehabilitation of Craniofacial Anomalies, University of São Paulo for treatment. She could not close her mouth and was dissatisfied with her complete dentures. Treatment planning comprised placement of six implants in the maxilla, four in the mandible followed by prostheses installation and orthognathic surgery. The mandibular full arch prosthesis guided the occlusion for orthognathic positioning of the maxilla. The maxillary complete prosthesis was designed to assist the orthognathic surgery with a provisional prosthesis (no metal framework), allowing reverse treatment planning. Maxillary and mandibular realignment was performed. Three months later, a relapse in the position of the maxilla was observed, which was offset with a new maxillary prosthesis. This isa complex interdisciplinary treatment and two-year follow-up is presented and discussed. It should be considered that this type of treatment could also be applied in non-cleft patients.


Subject(s)
Humans , Male , Female , Middle Aged , Aged , Aged, 80 and over , Aniline Compounds , Brain/blood supply , Brain/pathology , Cerebral Amyloid Angiopathy/complications , Cerebral Amyloid Angiopathy/diagnosis , Cerebral Hemorrhage/complications , Positron-Emission Tomography/methods , Thiazoles , Cerebral Hemorrhage/diagnosis
14.
Rev. paul. pediatr ; 33(1): 42-49, Jan-Mar/2015. tab, graf
Article in English | LILACS | ID: lil-744699

ABSTRACT

OBJECTIVE: To evaluate differences in children's eating behavior in relation to their nutritional status, gender and age. METHODS: Male and female children aged six to ten years were included. They were recruited from a private school in the city of Pelotas, Rio Grande do Sul, southern Brazil, in 2012. Children´s Eating Behaviour Questionnaire (CEBQ) subscales were used to assess eating behaviors: Food Responsiveness (FR), Enjoyment of Food (EF), Desire to Drink (DD), Emotional Overeating (EOE), Emotional Undereating (EUE), Satiety Responsiveness (SR), Food Fussiness (FF) and Slowness in Eating (SE). Age-adjusted body mass index (BMI) z-scores were calculated according to the WHO recommendations to assess nutritional status. RESULTS: The study sample comprised 335 children aged 87.9±10.4 months and 49.3% had normal weight (n=163), 26% were overweight (n=86), 15% were obese (n=50) and 9.7% were severely obese (n=32). Children with excess weight showed higher scores at the CEBQ subscales associated with "food approach" (FR, EF, DD, EOE, p<0.001) and lower scores on two "food avoidance" subscales (SR and SE, p<0.001 and p=0.003, respectively) compared to normal weight children. Differences in the eating behavior related to gender and age were not found. CONCLUSIONS: "Food approach" subscales were positively associated to excess weight in children, but no associations with gender and age were found. .


OBJETIVO: Avaliar diferenças no comportamento alimentar infantil em função do estado nutricional, do sexo e da idade. MÉTODOS: O estudo incluiu crianças na faixa de seis a dez anos, de ambos os sexos, de uma escola privada em Pelotas (RS), em 2012. Para avaliar o comportamento alimentar usaram-se as subescalas do questionário Children's Eating Behaviour Questionnaire (CEBQ): resposta à comida (FR), prazer de comer (EF), desejo de beber (DD), sobreingestão emocional (EOE), subingestão emocional (EUE), resposta à saciedade (SR), seletividade (FF) e ingestão lenta (SE). Avaliou-se o estado nutricional por meio do escore-z do IMC/idade. RESULTADOS: Participaram 335 crianças de 87,9±10,4 meses. Apresentaram eutrofia 49,3% (n=163), sobrepeso 26% (n=86), obesidade 15% (n=50) e obesidade grave 9,7% (n=32). Crianças com excesso de peso tiveram maior pontuação nas subescalas de "interesse pela comida" (FR, EF, DD, EOE, p<0,001) e menor pontuação nas subescalas de "desinteresse pela comida" (SR e SE, p<0,001 e p=0,003, respectivamente), se comparadas com as crianças com peso adequado. Não foram observadas diferenças no comportamento alimentar segundo sexo e idade. CONCLUSÕES: Observou-se que comportamentos alimentares que refletem "interesse pela comida" estão associados positivamente ao excesso de peso, mas não foi encontrada associação com o sexo e a idade da criança. .


Subject(s)
Animals , Female , Humans , Mice , Aniline Compounds/pharmacology , Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Drug Discovery , Microtubules/drug effects , Neoplasms, Experimental/drug therapy , Protein Kinase Inhibitors/pharmacology , Pyrimidines/pharmacology , Receptor Protein-Tyrosine Kinases/antagonists & inhibitors , Water/chemistry , Aniline Compounds/chemistry , Aniline Compounds/chemical synthesis , Antineoplastic Agents/chemistry , Antineoplastic Agents/chemical synthesis , Cell Line, Tumor , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , HeLa Cells , Mice, Inbred BALB C , Mice, Nude , Neoplasms, Experimental/pathology , Protein Kinase Inhibitors/chemistry , Protein Kinase Inhibitors/chemical synthesis , Pyrimidines/chemistry , Pyrimidines/chemical synthesis , Receptor Protein-Tyrosine Kinases/metabolism , Solubility , Structure-Activity Relationship
15.
Rev. paul. pediatr ; 33(1): 72-81, Jan-Mar/2015. tab, graf
Article in English | LILACS | ID: lil-744702

ABSTRACT

OBJECTIVE: To investigate the association between behavioral risk factors, specifically postural habits, with the presence of structural changes in the spinal column of children and adolescents. METHODS: 59 students were evaluated through the self-reporting Back Pain and Body Posture Evaluation Instrument and spinal panoramic radiographic examination. Spine curvatures were classified based on Cobb angles, as normal or altered in the saggital plane and as normal or scoliotic in the frontal plane. Data were analyzed using SPSS 18.0, based on descriptive statistics and chi-square association test (a=0,05). RESULTS: The prevalence of postural changes was 79.7% (n=47), of which 47.5% (n=28) showed frontal plane changes and 61% (n=36) sagital plane changes. Significant association was found between the presence of thoracic kyphosis and female gender, practice of physical exercises only once or twice a week, sleep time greater than 10 hours, inadequate postures when sitting on a seat and sitting down to write, and how school supplies are carried. Lumbar lordosis was associated with the inadequate way of carrying the school backpack (asymmetric); and scoliosis was associated wuth the practice of competitive sports and sleep time greater than 10 hours. CONCLUSIONS: Lifestyle may be associated with postural changes. It is important to develop health policies in order to reduce the prevalence of postural changes, by decreasing the associated risk factors. .


OBJETIVO: Verificar se existe associação de fatores de risco comportamentais, especificamente hábitos posturais, com a presença de alteração postural estrutural na coluna vertebral de crianças e adolescentes. MÉTODOS: Foram avaliados 59 crianças e adolescentes, que responderam ao questionário auto-aplicável Back Pain and Body Posture Evaluation Instrument e fizeram o exame radiográfico panorâmico da coluna vertebral. De acordo com o ângulo de Cobb, as curvaturas sagitais da coluna vertebral foram classificadas como normais ou alteradas e, no plano frontal, como escolioses ou normais. Os dados foram analisados no SPSS 18.0, a partir de estatística descritiva e do teste de associação qui-quadrado (a=0,05). RESULTADOS: A prevalência de alterações posturais foi de 79,7% (n=47), 47,5% (n=28) apresentavam alteração no plano frontal e 61% (n=36) no sagital. Foi encontrada associação entre cifose torácica e sexo feminino, prática de exercício físico apenas uma ou duas vezes na semana, tempo de sono superior a 10 horas, posturas inadequadas para sentar no banco e sentar para escrever e o meio de transporte do material escolar. Para lordose lombar, observou-se associação com o transporte da mochila escolar de modo inadequado (assimétrico). Houve associação significativa entre a presença de escoliose com a prática de esporte competitivo e o tempo de sono superior a 10 horas. CONCLUSÕES: Hábitos de vida podem estar associados a alterações posturais, é importante o desenvolvimento políticas de saúde a fim de reduzir a prevalência de alterações posturais por meio da redução dos fatores de risco associados. .


Subject(s)
Humans , Aniline Compounds/pharmacology , Protein Kinase Inhibitors/pharmacology , Quinolones/pharmacology , Aniline Compounds/chemistry , Aniline Compounds/chemical synthesis , Dose-Response Relationship, Drug , Models, Molecular , Molecular Structure , Protein Kinase Inhibitors/chemistry , Protein Kinase Inhibitors/chemical synthesis , Quinolones/chemistry , Quinolones/chemical synthesis , Structure-Activity Relationship
16.
Protein & Cell ; (12): 443-452, 2015.
Article in English | WPRIM | ID: wpr-757581

ABSTRACT

Voltage-gated sodium channels (VGSCs) in primary sensory neurons play a key role in transmitting pain signals to the central nervous system. BmK I, a site-3 sodium channel-specific toxin from scorpion Buthus martensi Karsch, induces pain behaviors in rats. However, the subtypes of VGSCs targeted by BmK I were not entirely clear. We therefore investigated the effects of BmK I on the current amplitude, gating and kinetic properties of Nav1.8, which is associated with neuronal hyperexcitability in DRG neurons. It was found that BmK I dose-dependently increased Nav1.8 current in small-sized (<25 μm) acutely dissociated DRG neurons, which correlated with its inhibition on both fast and slow inactivation. Moreover, voltage-dependent activation and steady-state inactivation curves of Nav1.8 were shifted in a hyperpolarized direction. Thus, BmK I reduced the threshold of neuronal excitability and increased action potential firing in DRG neurons. In conclusion, our data clearly demonstrated that BmK I modulated Nav1.8 remarkably, suggesting BmK I as a valuable probe for studying Nav1.8. And Nav1.8 is an important target related to BmK I-evoked pain.


Subject(s)
Animals , Male , Rats , Aniline Compounds , Pharmacology , Cell Size , Cells, Cultured , Electrophysiological Phenomena , Furans , Pharmacology , Ganglia, Spinal , Cell Biology , Kinetics , Metabolism , Rats, Sprague-Dawley , Scorpion Venoms , Pharmacology , Scorpions , Sensory Receptor Cells , Metabolism , Physiology , Sodium Channel Blockers , Pharmacology , Voltage-Gated Sodium Channel Agonists , Pharmacology
17.
Acta Pharmaceutica Sinica ; (12): 1613-1621, 2015.
Article in English | WPRIM | ID: wpr-320034

ABSTRACT

Thirteen of 4-anilinoquinazoline derivatives with imine groups at position 6 of quinazoline ring were synthesized and their antitumor activities were evaluated by MTT assay and Western blotting analysis. Among these compounds, 13a-131 were reported first time. The MTT assay was carried out on three human cancer cell lines (A549, HepG2 and SMMC7721) with EGFR highly expressed. Among the tested compounds, 13i and 13j exhibited notable inhibition potency and their IC50 values on three cell lines were equivalent to or less than those of gefitinib. Compound 14, without imine group substituted, displayed excellent inhibitor potency only on A549 cell line. Compounds 14 and 13j were chosen to perform Western blotting analysis on A549. The results showed that both of the compounds could inhibit the expression level of phosphorylated EGFR remarkably. It was concluded that the inhibitor potency of compound 14 was almost equivalent to that of gefitinib and the inhibitor potency of 13j was better than that of gefitinib.


Subject(s)
Humans , Aniline Compounds , Pharmacology , Antineoplastic Agents , Pharmacology , Cell Line, Tumor , Inhibitory Concentration 50 , Phosphorylation , Quinazolines , Pharmacology , ErbB Receptors
18.
Article in Chinese | WPRIM | ID: wpr-297406

ABSTRACT

<p><b>OBJECTIVE</b>To study whether the analgesis of oxymatrine (OMT) affects N-type voltage-gated calcium channels (VGCCs).</p><p><b>METHODS</b>Totally 45 mice were randomly divided into the sham-operation group, the model group [established by partial sciatic nerve ligation (PSNL)] , and the OMT treatment group according to random digit table, 15 in each group. The dorsal root ganglions (DRG) were separated in PSNL pain model mice. Intracellular calcium concentration ([Ca2+]i) was determined with Fluo-3 AM immunofluorescent probe in cultured DRG neurons. Different protein expression levels of N-type (Cav2. 2) and L-type ( Cav1. 3) among VGCCs from brain and DRG tissues were detected with Western blot.</p><p><b>RESULTS</b>Compared with the sham-operation group, [Ca2+]i, increased in cultured DRG neurons (P <0. 05) , protein expression levels of Cav2. 2 in the brain tissue increased (P <0. 05), protein expression levels of Cav2. 2 in DRG tissues decreased in the model group (P <0. 01). Compared with the model group, [Ca2+]i, decreased in cultured DRG neurons (P < 0. 05), protein expression levels of Cav2. 2 in the brain tissue decreased (P <0. 01), protein expression levels of Cav2. 2 in DRG tissues increased in the OMT treatment group (P <0. 01). There was no statistical difference in Cav1. 3 expressions in cultured DRG neurons and the brain (P >0. 05).</p><p><b>CONCLUSION</b>Analgesic effect of OMT might be related to Cav2. 2 channel mediated calcium ion flux.</p>


Subject(s)
Animals , Mice , Alkaloids , Pharmacology , Analgesia , Methods , Analgesics , Pharmacology , Aniline Compounds , Calcium , Calcium Channels, N-Type , Physiology , Ganglia, Spinal , Neurons , Pain , Quinolizines , Pharmacology , Xanthenes
19.
Chinese Journal of Cardiology ; (12): 254-258, 2015.
Article in Chinese | WPRIM | ID: wpr-328779

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of κ-opioid receptor (κ-OR) stimulation on Angiotensin II (Ang II)-induced cardiomyocyte hypertrophy in vitro cultured myocardial cells from neonatal rats and on calcineurin (CaN) signal pathways.</p><p><b>METHODS</b>Cultured myocardial cells of neonatal rats were divided into control group, CSA (1 µmol/L) group, Ang II (1 µmol/L) group, Ang II (1 µmol/L) + U50488H (1 µmol/L) group, Ang II (1 µmol/L) + CSA (1 µmol/L) group, Ang II (1 µmol/L) + Rp-cAMPS (1 µmol/L) group, Ang II (1 µmol/L) + CSA (1 µmol/L) + U50488H (1 µmol/L) group and Ang II (1 µmol/L) + PTX5 mg/L + U50488H (1 µmol/L) group. The hypertrophic myocytes were induced by Ang II 1µmol/L before κ-OR agonist U50488H 1 µmol/L was administered. The antihypertrophic effect of κ-OR stimulation was observed in the presence of ciclosporine A (CsA) 1 µmol/L, cAMP triethyl-ammonium salt (Rp-cAMPS) 1 µmol/L, and pertussistoxin ( PTX) 5 mg/L. The total protein content was assayed by the method of Lowry. The [Ca²⁺]i was measured by confocal microscope using Fluo-3/AM as flouresecent indicator. The relative expression of CaN was determined by Western blot.</p><p><b>RESULTS</b>(1) The total protein content of Ang II group was significantly higher than that in control group (P<0.01), which could be equally reduced by cotreatment with U50488H, CSA and Rp-cAMPS (P<0.01). Total protein content of the Ang II + PTX + U50488H group and the Ang II group was similar. (2) The [Ca²⁺]i was significantly higher in Ang II group of neonatal rat cardiomyocytes than that in control group (P<0.01), which could be reduced by cotreatment with U50488H, CSA and Rp-cAMPS (P<0.01). [Ca(2+)]i was similar between the Ang II + PTX + U50488H group and the Ang II group. (3) The expression of CaN was significantly higher in Ang II group than that in control group (P<0.01), which could be significantly reduced by cotreatment with U50488H, CSA and Rp-cAMPS (P<0.01). CaN was similar between the Ang II + PTX + U50488H group and the Ang II group.</p><p><b>CONCLUSION</b>κ-opioid receptor activation could attenuate Ang II induced cardiomyocytes hypertrophy via reducing [Ca²⁺]i and downreglating CaN.</p>


Subject(s)
Animals , Rats , 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer , Angiotensin II , Aniline Compounds , Animals, Newborn , Calcineurin , Cells, Cultured , Hypertrophy , Myocardium , Myocytes, Cardiac , Receptors, Opioid, kappa , Signal Transduction , Xanthenes
20.
Article in Chinese | WPRIM | ID: wpr-326005

ABSTRACT

<p><b>OBJECTIVE</b>To establish the method of high-performance liquid chromatography (HPLC) for the determination of N-isopropylaniline in workplace air.</p><p><b>METHODS</b>N-isopropylaniline in the air was collected by silicone tube, and was then dissolved by acetonitrile and determined by HPLC-UV detector.</p><p><b>RESULTS</b>There was a linear relationship within the range of 0.0-100.0 µg/ml with the method, and the regression equation was y=22 863x+10 665(r=0.999 9); the detection limit was 0.005 µg/ml, and the minimum detectable concentration was 1.7x10(-3) mg/m3 (3.0 L sampling volume); the average recoveries of standard addition were 96.2%-101.3%. The within-run precision was 2.31%-2.99%, and the between-run precision was 3.21%-4.55%. The average desorption efficiency was 97.6%, the breakthrough volume was more than 8.12 mg, the sampling efficiency waE 100%, and the samples could be stored for at least 7 days at room temperature.</p><p><b>CONCLUSION</b>The indicators ol the method all meet the requirements of GBZ/T 210.4-2008 (Determination methods of air chemicals in workplace), and can be used for the determination of N-isopropylaniline in workplace air.</p>


Subject(s)
Air Pollutants, Occupational , Aniline Compounds , Chromatography, High Pressure Liquid , Limit of Detection , Workplace
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