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2.
Mem. Inst. Oswaldo Cruz ; 115: e190396, 2020. graf
Article in English | LILACS | ID: biblio-1101277

ABSTRACT

BACKGROUND Nanoparticles (NPs) are viable candidates as carriers of exogenous materials into cells via transfection and can be used in the DNA vaccination strategy against leptospirosis. OBJECTIVES We evaluated the efficiency of halloysite clay nanotubes (HNTs) and amine-functionalised multi-walled carbon nanotubes (NH2-MWCNTs) in facilitating recombinant LemA antigen (rLemA) expression and protecting Golden Syrian hamsters (Mesocricetus auratus) against Leptospira interrogans lethal infection. METHODS An indirect immunofluorescent technique was used to investigate the potency of HNTs and NH2-MWCNTs in enhancing the transfection and expression efficiency of the DNA vaccine in Chinese hamster ovary (CHO) cells. Hamsters were immunised with two doses of vaccines HNT-pTARGET/lemA, NH2-MWCNTs-pTARGET/lemA, pTARGET/lemA, and empty pTARGET (control), and the efficacy was determined in terms of humoral immune response and protection against a lethal challenge. FINDINGS rLemA DNA vaccines carried by NPs were able to transfect CHO cells effectively, inducing IgG immune response in hamsters (p < 0.05), and did not exhibit cytotoxic effects. Furthermore, 83.3% of the hamsters immunised with NH2-MWCNTs-pTARGET/lemA were protected against the lethal challenge (p < 0.01), and 66.7% of hamsters immunised with HNT-pTARGET/lemA survived (p < 0.05). MAIN CONCLUSIONS NH2-MWCNTs and HNTs can act as antigen carriers for mammalian cells and are suitable for DNA nanovaccine delivery.


Subject(s)
Animals , Female , Bacterial Proteins/administration & dosage , Transcription Factors/administration & dosage , Bacterial Vaccines/administration & dosage , Vaccines, DNA/administration & dosage , Leptospirosis/prevention & control , Antigens, Bacterial/administration & dosage , Bacterial Proteins/immunology , Transcription Factors/immunology , Bacterial Vaccines/immunology , Cricetinae , Fluorescent Antibody Technique, Indirect , Vaccines, DNA/immunology , Disease Models, Animal , Nanoparticles , Leptospira interrogans/immunology , Leptospirosis/immunology , Antibodies, Bacterial/immunology , Antigens, Bacterial/immunology
3.
An. bras. dermatol ; 94(4): 405-410, July-Aug. 2019. tab, graf
Article in English | LILACS | ID: biblio-1038299

ABSTRACT

Abstract: Background: A high prevalence of leprosy among children under 15 years of age indicates the need to implement actions to prevent new cases of the disease. Serological tests have been developed with the aim of helping to control the disease by indicating, through seropositivity, the presence of infection. Objective: To analyze the prevalence and factors associated with seropositivity rate for anti-NDO-LID antibodies in children under 15 years of age, contacts of leprosy patients. Method: We performed a cross-sectional study with 210 children under 15 years old of age. Of them, 50 were household contacts and 160 were neighborhood contacts living in the municipality of Cuiabá, state of Mato Grosso, in 2016. The data were obtained from interviews and the NDO-LID rapid test during home visits from February to July 2016. For the analysis, we used Poisson regression and prevalence ratio. Results: Seropositivity in contacts was 6.2%. Variables associated with seropositive tests included sex (PR = 1.05; 95% CI: 1.01 - 1.08), race/skin color (PR = 0.95; 95% CI: 0.90 - 0.99), residence area (PR = 1.05; 95% CI: 1.01 - 1.09), and number of people per household (PR = 1.06; 95% CI: 1.02 - 1.08). Study Limitations: The small sample size, besides leading to wide confidence intervals, may have been a limitation for the identification of associated factors. Conclusions: The prevalence of seropositivity was high. Variables associated with NDO-LID seropositivity included female sex, not to be brown skinned, live in urban areas, and live with five or more people.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Antibodies, Anti-Idiotypic/immunology , Leprosy/immunology , Leprosy/epidemiology , Antibodies, Bacterial/immunology , Socioeconomic Factors , Brazil/epidemiology , Serologic Tests/methods , Residence Characteristics , Family Characteristics , Antibodies, Anti-Idiotypic/blood , Cross-Sectional Studies , Age Factors , Sex Distribution , Age Distribution , Infant , Antibodies, Bacterial/blood
4.
Braz. j. microbiol ; 49(4): 795-800, Oct.-Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-974314

ABSTRACT

ABSTRACT The objective of this study was to evaluate the occurrence of anti-Leptospira spp. antibodies in female buffalo in the state of Pernambuco. A total of 123 female buffalo blood samples were collected from five properties distributed in the state of Pernambuco. The microscopic agglutination test was used to study anti-Leptospira spp. antibodies. The occurrence of anti-Leptospira spp. antibodies was 28.5% (35/123; CI 20.7-37.3%) and on different properties, the occurrence ranged from 28.6% to 80.0%, with 100% of the properties showing animals with positive results. The serovars of the serogroup Sejroe with a higher incidence were Hardjoprajtino (CTG strain, 49.1%) and Hardjo (Prajtino genotype, 43.2%), followed by serogroup Grippotyphosa with the Grippotyphosa serovar (3.9%), serogroup Pomona with the Pomona serovar (1.9%), and the Icterohaemorrhagiae serovar Copenhageni (1.9%). This was the first record of the occurrence of anti-Lepstospira spp. antibodies in female buffalo in the state of Pernambuco. Control measures are necessary to prevent health and economic losses, given that the agent involved affects animal reproduction, triggering drops in conception rates or even clinical cases of abortion.


Subject(s)
Animals , Female , Cattle , Buffaloes/microbiology , Cattle Diseases/blood , Leptospira/immunology , Leptospirosis/veterinary , Antibodies, Bacterial/blood , Brazil , Agglutination Tests , Buffaloes/immunology , Cattle Diseases/immunology , Cattle Diseases/microbiology , Serogroup , Leptospira/isolation & purification , Leptospira/genetics , Leptospirosis/immunology , Leptospirosis/microbiology , Leptospirosis/blood , Antibodies, Bacterial/immunology
5.
Braz. j. microbiol ; 49(2): 320-328, Apr.-June 2018. tab, graf
Article in English | LILACS | ID: biblio-889239

ABSTRACT

Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. The results showed that the PagC polyclonal antiserum is of good specificity and the capture rate of 0.1 mg IMBs for Salmonella tended to be stable at the range of 70-74% corresponding to the concentrations between 101 and 104 CFU/mL. The method developed demonstrated high specificity for the positive Salmonella samples when compared to non-specific DNA samples, such as Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica, and Yersinia pseudotuberculosis. The limit of detection of this assay was 18 CFU/mL. Detection and quantitative enumeration of Salmonella in samples of pork or milk shows good recoveries of 54.34% and 52.07%. In conclusion, the polyclonal antibody of recombinant PagC protein is effective to capture Salmonella from detected samples. The developed pagC antibody IMBs-qPCR method showed efficiency, sensitivity and specificity for 30 Salmonella detection, enabling detection within 10 h, which is a promising rapid method to detect Salmonella in emergency.


Subject(s)
Animals , Salmonella/isolation & purification , Food Contamination , Immunomagnetic Separation/methods , Real-Time Polymerase Chain Reaction/methods , Food Microbiology/methods , Salmonella/genetics , Bacterial Proteins/immunology , Sensitivity and Specificity , Milk/microbiology , Meat/microbiology , Antibodies, Bacterial/immunology , Antibodies, Bacterial/metabolism
6.
Braz. j. med. biol. res ; 51(5): e6773, 2018. graf
Article in English | LILACS | ID: biblio-889077

ABSTRACT

Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted protection in C57BL/6 mice by reducing the bacterial, which was correlated with increased serum concentration of IgG antibodies (IgG1 and IgG2a) against S. aureus. The increase in IgG2a antibody levels was correlated with a decrease of bacterial load in intradermally immunized C57BL/6 mice, along with production of IL-17A at the inflammation site, as well as IgG1consumption. Similar results were not found in the A/j lineage. In conclusion, a vaccine against S. aureus should focus more on the individual characteristics of the host because it is a determinant factor for the success of the immunization.


Subject(s)
Animals , Mice , Antibodies, Bacterial/immunology , Methicillin-Resistant Staphylococcus aureus/immunology , Staphylococcal Infections/prevention & control , Staphylococcal Vaccines/immunology , Bacterial Load , Cytokines/immunology , Disease Models, Animal , Immunoglobulin G/immunology , Mice, Inbred C57BL , Staphylococcal Infections/immunology , Staphylococcal Vaccines/administration & dosage , Time Factors
7.
Braz. j. microbiol ; 48(4): 774-781, Oct.-Dec. 2017. tab, graf
Article in English | LILACS | ID: biblio-889161

ABSTRACT

ABSTRACT Food-borne diseases, caused by the pathogenic bacteria, are highly prevalent in the world. Salmonella is one of the most important bacterial genera responsible for this. Salmonella Enteritidis (SE) is one of the non-typhoid Salmonellae that can be transmitted to human from poultry products, water, and contaminated food. In recent years, new and rapid detection methods such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) have been developed. In this study, recombinant FliC (rFliC) was produced to be used as an antigen. The immunization was conducted in mice with the purified recombinant FliC (rFliC). The mice were subcutaneously immunized with rFliC and elicited significant rFliC specific serum IgG antibodies. An indirect ELISA system was established for the detection of Salmonella Enteritidis. Our results confirmed that the recombinant flagellin can be one of the excellent indicators for the detection of Salmonella Enteritidis.


Subject(s)
Humans , Animals , Mice , Enzyme-Linked Immunosorbent Assay/methods , Flagellin/analysis , Salmonella enteritidis/isolation & purification , Antibodies, Bacterial/analysis , Antibodies, Bacterial/immunology , Antigens, Bacterial/analysis , Antigens, Bacterial/immunology , Bacterial Proteins/analysis , Bacterial Proteins/genetics , Bacterial Proteins/immunology , Flagellin/genetics , Flagellin/immunology , Mice, Inbred BALB C , Salmonella enteritidis/genetics , Salmonella enteritidis/immunology
8.
J. pediatr. (Rio J.) ; 93(5): 532-537, Sept.-Oct. 2017. tab
Article in English | LILACS | ID: biblio-894054

ABSTRACT

Abstract Objective: HIV-infected individuals (HIVI) are threatened by meningococcal infection and presented lower response to vaccines. Data are scarce on long-term persistence of human serum bactericidal antibody (hSBA) after a meningococcal C conjugate (MCC) vaccine in HIVI youth; the authors aimed to describe this persistence in HIVI. Methods: HIVI and HIV uninfected individuals (HIVU), aged 2-18 years, CD4 >15% were recruited. Seroprotection (hSBA ≥1:4) at baseline and at 12-18 months after immunization was evaluated and the association of the different factors with the long-term persistence was calculated using logistic regression. Results: A total of 145 HIVI, 50 HIVU were recruited and immunized, and their median age was 11 years (median age in HIVI group was 12 years, and 10 years in HIVU group, p-value = 0.02). 85 HIVI (44%) had undetectable viral load (UVL). Seroprotection rate was 27.2%: 24.1% in HIVI and 36% in HIVU 12-18 months after immunization (p = 0.14). Baseline immunity (odds ratio [OR] = 70.70, 95% CI: 65.2-766.6); UVL at entry (OR: 2.87, 95% CI: 0.96-8.62) and lower family income (OR: 0.09, 95% CI: 0.01-0.69) were associated with seroprotection among HIVI. Conclusion: Seroprotection at 12-18 months after single dose of MCC was low for both groups, and higher among individuals who presented baseline immunity. Among HIVI, vaccine should be administered after UVL is achieved.


Resumo Objetivo: As pessoas infectadas pelo HIV (HIVI) estão sujeitas a infecção meningocócica e apresentam menor resposta a vacinas. São escassos os dados a respeito da persistência de longo prazo do anticorpo bactericida no soro humano (hSBA) após vacina conjugada meningocócica C (MCC) em HIVI jovens e visamos a descrever essa persistência em HIVI. Métodos: Foram recrutadas pessoas HIVI e pessoas não infectadas por HIV (HIVU), entre 2 e 18 anos, CD4 > 15%. A seroproteção (hSBA ≥ 1:4) basal aos 12-18 meses após a imunização foi avaliada e a associação dos diferentes fatores com a persistência de longo prazo foi calculada com a regressão logística. Resultados: Foram recrutados 145 HIVI e 50 HIVU e imunizados e sua idade média foi determinada em 11 anos (12 no grupo HIVI e 10 no grupo HIVU, valor de p = 0,02); 85 HIVI (44%) apresentaram carga viral indetectável (CVI). A taxa de seroproteção foi 27,2%: 24,1% no grupo HIVI e 36% no grupo HIVU 12-18 meses após imunização (p = 0,14). A imunidade basal [razão de chance (RC) = 7070, IC: 65,2-7666]; CVI no momento da participação (RC: 2,87, IC de 95%: 0,96-8,62) e renda familiar mais baixa (RC: 0,09, IC de 95%: 0,01-0,69) foram associadas a seroproteção entre as pessoas HIVI. Conclusão: A seroproteção aos 12-18 meses após única dose de MCC mostrou-se baixa em ambos os grupos e mais elevada entre as pessoas que apresentaram imunidade basal. Entre as pessoas HIVI, as vacinas devem ser administradas após a CVI ser atingida.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Acquired Immunodeficiency Syndrome/immunology , Meningococcal Vaccines/immunology , Meningococcal Infections/prevention & control , Antibodies, Bacterial/immunology , Time Factors , Case-Control Studies , Meningococcal Vaccines/administration & dosage , Antibodies, Bacterial/blood
9.
Einstein (Säo Paulo) ; 15(3): 256-261, July-Sept. 2017. tab, graf
Article in English | LILACS | ID: biblio-891403

ABSTRACT

ABSTRACT Objective To describe e compare the specificity of IgA antibodies against bacteria extract of Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli , and Salmonella enteritidis . Methods Colostrum samples were aseptically collected in the first 12 hours after C-section delivery. The specificity of IgA against bacteria extracts was analyzed by the Western blot. Results The findings showed proteins of high molecular weight frequently detectable in the samples. S. aureus was the most frequently found bacterium in the samples (p<0.05). Approximately 93.8, 56.3, 62.5 and 60.4% of samples presented IgA reactive to S. aureus , K. pneumoniae , S. enteritidis, and E. coli, respectively. Roughly 40% of samples showed no IgA reactive to K. pneumoniae, S. enteritidis and E. coli . Conclusion Clinical evidence of the importance of breastfeeding for the immune protection of neonates was consistent with the observed immunological findings, since most samples showed IgA reactive against the species tested. The application and development of immunotherapies during pregnancy, focused on frequently detected antigens, could be an important tool to enhance the presence of IgA in colostrum.


RESUMO Objetivo Descrever e comparar a especificidade de anticorpos IgA de amostras de colostro contra extratos bacterianos de Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli e Salmonella enteritidis . Métodos As amostras de colostro foram coletadas assepticamente nas primeiras 12 horas após o nascimento por cesariana. A especificidade de IgA contra extratos de bactérias foi analisada por Western blot. Resultados Os achados mostraram proteínas de alto peso molecular frequentemente detectáveis nas amostras. S. aureus foi a bactéria mais encontrada nas amostras (p<0,05). Cerca de 93,8, 56,3, 62,5 e 60,4% das amostras apresentaram IgA reativa a S. aureus , K. pneumoniae , S. enteritidis e E. coli , respectivamente. Aproximadamente 40% das amostras não apresentaram IgA reativa contra K. pneumoniae , S. enteritidis e E. coli. Conclusão A evidência clínica da importância da amamentação para proteção imunológica ao recém-nascido foi consistente com os achados imunológicos observados, uma vez que a maioria das amostras mostrou IgA reativa contra as espécies testadas. A aplicação e o desenvolvimento de imunoterapias durante a gestação, focada nos antígenos frequentemente detectados, poderiam ser importantes instrumentos para aumentar a presença de IgA no colostro.


Subject(s)
Humans , Female , Infant, Newborn , Salmonella enteritidis/immunology , Staphylococcus aureus/immunology , Immunoglobulin A, Secretory/analysis , Colostrum/immunology , Escherichia coli/immunology , Klebsiella pneumoniae/immunology , Antibodies, Bacterial/analysis , Immunoglobulin A, Secretory/immunology , Blotting, Western , Sensitivity and Specificity , Antibodies, Bacterial/immunology
10.
Clinics ; 71(12): 687-694, Dec. 2016. tab, graf
Article in English | LILACS | ID: biblio-840027

ABSTRACT

OBJECTIVE: To investigate the transmission of anti-Staphylococcus aureus (Sa) IgG, IgG1 and IgG2 via placental transfer and the transfer of IgA via the colostrum according to maternal Sa carrier status at delivery. METHODS: We evaluated anti-Sa IgG, IgG1 and IgG2 in maternal and cord sera and IgA in colostrum from a case (n=49, Sa+) and a control group (n=98, Sa-). RESULTS: Of the 250 parturients analyzed for this study, 49 were nasally colonized with S. aureus (prevalence of 19.6%). Ninety-eight non-colonized subjects were selected for the control group. The anti-Sa IgG, IgG1 and IgG2 levels and the IgG avidity indexes in the maternal and cord sera did not differ between the groups, with a low transfer ratio of anti-Sa IgG to the newborns in both groups. The anti-Sa IgG2 titers were significantly higher than the IgG1 titers in the maternal and cord sera. Inversely, the transfer ratios were higher for anti-Sa IgG1 compared with IgG2; however, no differences between the groups were detected. The Sa-specific IgA levels and avidity indexes in the colostrum were equivalent between groups. CONCLUSIONS: Maternal Sa nasal colonization at delivery is not associated with higher antibody levels in the mother or newborns. The high titers of anti-Sa IgG2 found in the cord serum indicate a greater reactivity with non-protein antigens, which may further contribute to the susceptibility to staphylococcal infections at birth. The presence of IgA in the colostrum with avidity to S. aureus reinforces the importance of breastfeeding shortly after birth.


Subject(s)
Humans , Female , Pregnancy , Infant, Newborn , Adult , Placenta/immunology , Staphylococcus aureus/immunology , Breast Feeding , Immunoglobulin G/blood , Immunity, Maternally-Acquired/immunology , Antibodies, Bacterial/blood , Reference Values , Staphylococcus aureus/isolation & purification , Umbilical Cord/immunology , Immunoglobulin G/immunology , Enzyme-Linked Immunosorbent Assay , Cross-Sectional Studies , Colostrum/immunology , Statistics, Nonparametric , Antibodies, Bacterial/immunology
11.
Braz. j. microbiol ; 47(2): 394-402, Apr.-June 2016. tab, graf
Article in English | LILACS | ID: lil-780824

ABSTRACT

Abstract Clostridium difficile has emerged as an increasingly important nosocomial pathogen and the prime causative agent of antibiotic-associated diarrhoea and pseudomembranous colitis in humans. In addition to toxins A and B, immunological studies using antisera from patients infected with C. difficile have shown that a number of other bacterial factors contribute to the pathogenesis, including surface proteins, which are responsible for adhesion, motility and other interactions with the human host. In this study, various clostridial targets, including FliC, FliD and cell wall protein 66, were expressed and purified. Phage antibody display yielded a large panel of specific recombinant antibodies, which were expressed, purified and characterised. Reactions of the recombinant antibodies with their targets were detected by enzyme-linked immunosorbent assay; and Western blotting suggested that linear rather than conformational epitopes were recognised. Binding of the recombinant antibodies to surface-layer proteins and their components showed strain specificity, with good recognition of proteins from C. difficile 630. However, no reaction was observed for strain R20291—a representative of the 027 ribotype. Binding of the recombinant antibodies to C. difficile M120 extracts indicated that a component of a surface-layer protein of this strain might possess immunoglobulin-binding activities. The recombinant antibodies against FliC and FliD proteins were able to inhibit bacterial motility.


Subject(s)
Humans , Bacterial Proteins/analysis , Clostridioides difficile/genetics , Clostridium Infections/microbiology , Antibodies, Bacterial/analysis , Bacterial Proteins/genetics , Bacterial Proteins/immunology , Recombinant Proteins/analysis , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Gene Expression , Blotting, Western , Clostridioides difficile/isolation & purification , Clostridioides difficile/immunology , Clostridium Infections/diagnosis , Ribotyping , Antibodies, Bacterial/genetics , Antibodies, Bacterial/immunology
12.
Mem. Inst. Oswaldo Cruz ; 110(7): 914-920, Nov. 2015. tab, graf
Article in English | LILACS | ID: lil-764594

ABSTRACT

Leprosy inflammatory episodes [type 1 (T1R) and type 2 (T2R) reactions] represent the major cause of irreversible nerve damage. Leprosy serology is known to be influenced by the patient’s bacterial index (BI) with higher positivity in multibacillary patients (MB) and specific multidrug therapy (MDT) reduces antibody production. This study evaluated by ELISA antibody responses to leprosy Infectious Disease Research Institute diagnostic-1 (LID-1) fusion protein and phenolic glycolipid I (PGL-I) in 100 paired serum samples of 50 MB patients collected in the presence/absence of reactions and in nonreactional patients before/after MDT. Patients who presented T2R had a median BI of 3+, while MB patients with T1R and nonreactional patients had median BI of 2.5+ (p > 0.05). Anti-LID-1 and anti-PGL-I antibodies declined in patients diagnosed during T1R (p < 0.05). Anti-LID-1 levels waned in MB with T2R at diagnosis and nonreactional MB patients (p < 0.05). Higher anti-LID-1 levels were seen in patients with T2R at diagnosis (vs. patients with T1R at diagnosis, p = 0.008; vs. nonreactional patients, p = 0.020) and in patients with T2R during MDT (vs. nonreactional MB, p = 0.020). In MB patients, high and persistent anti-LID-1 antibody levels might be a useful tool for clinicians to predict which patients are more susceptible to develop leprosy T2R.


Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Antibodies, Bacterial/blood , Antigens, Bacterial/immunology , Glycolipids/immunology , Immunoglobulin M/blood , Leprosy, Multibacillary/diagnosis , Antibodies, Bacterial/immunology , Disease Susceptibility , Enzyme-Linked Immunosorbent Assay , Immunoglobulin M/immunology , Mycobacterium leprae/immunology
13.
Ciênc. saúde coletiva ; 20(2): 441-448, 02/2015. tab
Article in Portuguese | LILACS | ID: lil-742219

ABSTRACT

Objetivou-se analisar as internações por condições sensíveis à atenção primária (ICSAP) específicas em mulheres e os fatores que determinam ou influenciam a ocorrência dessas internações (fatores socioeconômicos, sociodemográficos e controle de saúde) por meio de um inquérito de morbidade hospitalar realizado com amostra de 429 mulheres internadas em hospitais conveniados ao Sistema Único de Saúde. O percentual de ICSAP foi 49,42% (n = 212), com destaque para as internações específicas do sexo feminino 19,35% (n = 83). Associaram ao risco de internar por CSAP: idade superior a 60 anos, baixa escolaridade, internação prévia, realização de controle regular de saúde, falta de vínculo com a Estratégia Saúde da Família (ESF) e ser gestante. As causas evidentes foram as condições relacionadas à gravidez, ao parto e ao puerpério e às inflamações nos órgãos pélvicos femininos. Os resultados sugerem falhas no atendimento ambulatorial que deveria ser oportuno e resolutivo no contexto da saúde da mulher.


The scope of this paper was to analyze female-specific sensitive hospitalization occurring in primary care conditions and factors that determine or affect the occurrence of such hospitalizations (social, economic and demographic factors; health control). Analysis was performed by surveys on hospital morbidity with a sample of 429 females attended in Unified Health System (SUS) contracted hospitals. The sensitive hospitalizations percentage in primary care reached 49.42% (n = 212), highlighting female-specific hospitalization at 19.35% (n = 83). Hospitalization risks comprised elderly people over sixty, low schooling, previous hospitalizations, normal health control, lack of association with the Family Health Strategy and pregnancy. Evident causes were related to conditions of pregnancy, childbirth, post-partum and inflammations of the female pelvic organs. Results suggested flaws in outpatient attendance that should be adequate and provide solutions in women’s health.


Subject(s)
Humans , Infant , Bacterial Proteins/immunology , Carrier Proteins/immunology , Diphtheria-Tetanus-Pertussis Vaccine/adverse effects , Diphtheria-Tetanus-Pertussis Vaccine/immunology , Haemophilus Vaccines/adverse effects , Haemophilus Vaccines/immunology , Immunoglobulin D/immunology , Lipoproteins/immunology , Pneumococcal Vaccines/adverse effects , Pneumococcal Vaccines/immunology , Poliovirus Vaccine, Inactivated/adverse effects , Poliovirus Vaccine, Inactivated/immunology , Antibodies, Bacterial/immunology , Antibodies, Viral/immunology , Diphtheria-Tetanus-Pertussis Vaccine/administration & dosage , Haemophilus Vaccines/administration & dosage , Immunization Schedule , Netherlands , Pneumococcal Vaccines/administration & dosage , Poliovirus Vaccine, Inactivated/administration & dosage , Vaccination , Vaccines, Conjugate , Vaccines, Combined/administration & dosage , Vaccines, Combined/adverse effects , Vaccines, Combined/immunology
14.
Article in English | WPRIM | ID: wpr-141165

ABSTRACT

Differentiated HL-60 is an effector cell widely used for the opsonophagocytic-killing assay (OPKA) to measure efficacy of pneumococcal vaccines. We investigated the correlation between phenotypic expression of immunoreceptors and phagocytic ability of HL-60 cells differentiated with N,N-dimethylformamide (DMF), all-trans retinoic acid (ATRA), or 1alpha, 25-dihydroxyvitamin D3 (VitD3) for 5 days. Phenotypic change was examined by flow cytometry with specific antibodies to CD11c, CD14, CD18, CD32, and CD64. Apoptosis was determined by flow cytometry using 7-aminoactinomycin D. Function was evaluated by a standard OPKA against serotype 19F and chemiluminescence-based respiratory burst assay. The expression of CD11c and CD14 gradually increased upon exposure to all three agents, while CD14 expression increased abruptly after VitD3. The expression of CD18, CD32, and CD64 increased during differentiation with all three agents. Apoptosis remained less than 10% until day 3 but increased after differentiation by DMF or ATRA. Differentiation with ATRA or VitD3 increased the respiratory burst after day 4. DMF differentiation showed a high OPKA titer at day 1 which sustained thereafter while ATRA or VitD3-differentiated cells gradually increased. Pearson analysis between the phenotypic changes and OPKA titers suggests that CD11c might be a useful differentiation marker for HL-60 cells for use in pneumococcal OPKA.


Subject(s)
Antibodies, Bacterial/immunology , CD11c Antigen/metabolism , Lipopolysaccharide Receptors/metabolism , CD18 Antigens/metabolism , Apoptosis/immunology , Biological Assay , Cell Differentiation , Cell Line, Tumor , Cholecalciferol/pharmacology , Dimethylformamide/pharmacology , Flow Cytometry , HL-60 Cells , Humans , Phagocytosis/immunology , Pneumococcal Vaccines/immunology , Receptors, IgG/metabolism , Receptors, Immunologic/biosynthesis , Respiratory Burst/immunology , Streptococcus pneumoniae/immunology , Tretinoin/pharmacology
15.
Article in English | WPRIM | ID: wpr-141164

ABSTRACT

Differentiated HL-60 is an effector cell widely used for the opsonophagocytic-killing assay (OPKA) to measure efficacy of pneumococcal vaccines. We investigated the correlation between phenotypic expression of immunoreceptors and phagocytic ability of HL-60 cells differentiated with N,N-dimethylformamide (DMF), all-trans retinoic acid (ATRA), or 1alpha, 25-dihydroxyvitamin D3 (VitD3) for 5 days. Phenotypic change was examined by flow cytometry with specific antibodies to CD11c, CD14, CD18, CD32, and CD64. Apoptosis was determined by flow cytometry using 7-aminoactinomycin D. Function was evaluated by a standard OPKA against serotype 19F and chemiluminescence-based respiratory burst assay. The expression of CD11c and CD14 gradually increased upon exposure to all three agents, while CD14 expression increased abruptly after VitD3. The expression of CD18, CD32, and CD64 increased during differentiation with all three agents. Apoptosis remained less than 10% until day 3 but increased after differentiation by DMF or ATRA. Differentiation with ATRA or VitD3 increased the respiratory burst after day 4. DMF differentiation showed a high OPKA titer at day 1 which sustained thereafter while ATRA or VitD3-differentiated cells gradually increased. Pearson analysis between the phenotypic changes and OPKA titers suggests that CD11c might be a useful differentiation marker for HL-60 cells for use in pneumococcal OPKA.


Subject(s)
Antibodies, Bacterial/immunology , CD11c Antigen/metabolism , Lipopolysaccharide Receptors/metabolism , CD18 Antigens/metabolism , Apoptosis/immunology , Biological Assay , Cell Differentiation , Cell Line, Tumor , Cholecalciferol/pharmacology , Dimethylformamide/pharmacology , Flow Cytometry , HL-60 Cells , Humans , Phagocytosis/immunology , Pneumococcal Vaccines/immunology , Receptors, IgG/metabolism , Receptors, Immunologic/biosynthesis , Respiratory Burst/immunology , Streptococcus pneumoniae/immunology , Tretinoin/pharmacology
17.
Rev. Soc. Bras. Med. Trop ; 47(1): 105-107, Jan-Feb/2014. tab, graf
Article in English | LILACS | ID: lil-703159

ABSTRACT

Introduction: The aim of this study was to evaluate the serological cross-reactivity between Leishmania sp. and other canine pathogens. Methods: Positive serum samples for Ehrlichia canis, Babesia canis, Toxoplasma gondii, Neospora caninum and Trypanosoma cruzi were tested using three serological methods enzyme linked immunosorbent assay (ELISA), indirect immunofluorescent antibody test (IFAT) and Kalazar Detect™, for canine visceral leishmaniasis. Results: Of the 57 dog samples tested, 24 (42.1%) tested positive using one of the three serological methods: 10/57 (17.5%) for ELISA, 11/57 (19.3%) for IFAT and 3/57 (5.3%) for Kalazar Detect™. Conclusions: Our results demonstrated that the presence of other infectious agents may lead to cross-reactivity on leishmaniasis serological tests. .


Subject(s)
Animals , Dogs , Antibodies, Bacterial/blood , Antibodies, Protozoan/blood , Dog Diseases/parasitology , Leishmaniasis, Visceral/parasitology , Antibodies, Bacterial/immunology , Antibodies, Protozoan/immunology , Babesia/immunology , Cross Reactions , Ehrlichia canis/immunology , Enzyme-Linked Immunosorbent Assay/veterinary , Fluorescent Antibody Technique, Indirect/veterinary , Leishmania infantum/immunology , Neospora/immunology , Toxoplasma/immunology , Trypanosoma cruzi/immunology
18.
Mem. Inst. Oswaldo Cruz ; 108(5): 668-670, ago. 2013. tab
Article in English | LILACS | ID: lil-680761

ABSTRACT

Mice experimentally infected with a pathogenic strain of Leptospira interrogans serovar Canicola produced false negative results (prozone effect) in a microscopic agglutination test (MAT). This prozone effect occurred in several serum samples collected at different post-infection times, but it was more prominent in samples collected from seven-42 days post-infection and for 1:50 and 1:100 sample dilutions. This phenomenon was correlated with increased antibody titres in the early post-infection phase. While prozone effects are often observed in serological agglutination assays for the diagnosis of animal brucellosis and human syphilis, they are not widely reported in leptospirosis MATs.


Subject(s)
Animals , Female , Humans , Mice , Leptospira interrogans serovar canicola/immunology , Leptospirosis/diagnosis , Agglutination Tests , Antibodies, Bacterial/blood , Antibodies, Bacterial/immunology , Enzyme-Linked Immunosorbent Assay , Leptospirosis/microbiology
19.
Braz. j. med. biol. res ; 46(2): 148-153, 01/fev. 2013. graf
Article in English | LILACS | ID: lil-668770

ABSTRACT

Serogroup B Neisseria meningitidis (MenB) is a major cause of invasive disease in early childhood worldwide. The only MenB vaccine available in Brazil was produced in Cuba and has shown unsatisfactory efficacy when used to immunize millions of children in Brazil. In the present study, we compared the specific functional antibody responses evoked by the Cuban MenB vaccine with a standard vaccine against diphtheria (DTP: diphtheria, tetanus, pertussis) after primary immunization and boosting of mice. The peak of bactericidal and opsonic antibody titers to MenB and of neutralizing antibodies to diphtheria toxoid (DT) was reached after triple immunization with the MenB vaccine or DTP vaccine, respectively. However, 4 months after immunization, protective DT antibody levels were present in all DTP-vaccinated mice but in only 20% of the mice immunized against MenB. After 6 months of primary immunization, about 70% of animals still had protective neutralizing DT antibodies, but none had significant bactericidal antibodies to MenB. The booster doses of DTP or MenB vaccines produced a significant antibody recall response, suggesting that both vaccines were able to generate and maintain memory B cells during the period studied (6 months post-triple immunization). Therefore, due to the short duration of serological memory induced by the MenB vaccine (VA-MENGOC-BC® vaccine), its use should be restricted to outbreaks of meningococcal disease.


Subject(s)
Animals , Female , Mice , Antibodies, Bacterial/immunology , Diphtheria Toxoid/immunology , Diphtheria-Tetanus-Pertussis Vaccine/immunology , Meningococcal Infections/immunology , Neisseria meningitidis/immunology , Antibody Formation , Antigens, Bacterial/immunology , Immunologic Memory , Meningococcal Infections/prevention & control , Meningococcal Vaccines/immunology , Time Factors
20.
Mem. Inst. Oswaldo Cruz ; 107(supl.1): 60-67, Dec. 2012. ilus, mapas, tab
Article in English | SES-SP, LILACS, SES-SP, HANSEN, HANSENIASE, SESSP-ILSLPROD, SES-SP, SESSP-ILSLACERVO, SES-SP | ID: lil-659742

ABSTRACT

Leprosy in children is correlated with community-level factors, including the recent presence of disease and active foci of transmission in the community. We performed clinical and serological examinations of 1,592 randomly selected school children (SC) in a cross-sectional study of eight hyperendemic municipalities in the Brazilian Amazon Region. Sixty-three (4%) SC, with a mean age of 13.3 years (standard deviation = 2.6), were diagnosed with leprosy and 777 (48.8%) were seropositive for anti-phenolic glycolipid-I (PGL-I). Additionally, we evaluated 256 house-hold contacts (HHCs) of the students diagnosed with leprosy; 24 (9.4%) HHC were also diagnosed with leprosy and 107 (41.8%) were seropositive. The seroprevalence of anti-PGL-I was significantly higher amongst girls, students from urban areas and students from public schools (p < 0.0001). Forty-five (71.4%) new cases detected amongst SC were classified as paucibacillary and 59 (93.6%) patients did not demonstrate any degree of physical disability at diagnosis. The results of this study suggest that there is a high rate of undiagnosed leprosy and subclinical infection amongst children in the Amazon Region. The advantages of school surveys in hyperendemic areas include identifying leprosy patients at an early stage when they show no physical disabilities, preventing the spread of the infection in the community and breaking the chain of transmission.


Subject(s)
Humans , Male , Female , Child , Adolescent , Leprosy, Multibacillary/diagnosis , Leprosy, Paucibacillary/diagnosis , Antibodies, Bacterial/immunology , Antigens, Bacterial/blood , Asymptomatic Infections/epidemiology , Brazil/epidemiology , Cross-Sectional Studies , Enzyme-Linked Immunosorbent Assay , Glycolipids/blood , Leprosy, Multibacillary/epidemiology , Leprosy, Paucibacillary/epidemiology , Mycobacterium leprae/immunology , Seroepidemiologic Studies , Students
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