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Braz. j. infect. dis ; 23(3): 151-159, May-June 2019. tab
Article in English | LILACS | ID: biblio-1019551


ABSTRACT Background: HIV infection harms adaptive cellular immunity mechanisms. Long-term virological control by combined antiretroviral therapy (cART) reduces the risk of mycobacterial infections. Thus, we aimed to study cellular responses to mycobacterial antigens in 20 HIV-infected adolescents with at least one year of virological control (HIV-RNA <40 copies/mL) and 20 healthy adolescents. Methods: We evaluated CD8 and γδ T-cell degranulation by measurement of CD107a membrane expression after stimulation with lysates from BCG (10 µg/mL) and H37RA Mycobacterium tuberculosis (Mtb, 10 µg/mL). Immune activation and antigen-presenting ability were also assessed by determination of HLA-DR, CD80, and CD86 markers. Results: TCR γδ T-cell CD107a expression was similar between groups in response to mycobacterial antigens, and lower in the HIV-infected group in response to mitogen. Higher baseline HLA-DR expression and lower mycobacterial-stimulated expression was found within the HIV-infected group. Conclusions: Similar degranulation in stimulated CD8+ and TCR γδ T-cells from HIV-infected adolescents, when compared to healthy controls suggests long-term immunological preservation with immune reconstitution under successful cART. However, differences in HLA-DR expression may represent ongoing inflammation and lower specific responses in HIV-infected youth. These features may be relevant in the context of the precocity and severity of vertically acquired HIV infection.

Humans , Male , Female , Young Adult , Receptors, Antigen, T-Cell, alpha-beta/immunology , AIDS-Related Opportunistic Infections/immunology , CD8-Positive T-Lymphocytes/immunology , Anti-HIV Agents/therapeutic use , Mycobacterium tuberculosis/immunology , Antigens, Bacterial/immunology , Tuberculosis/immunology , Biomarkers/blood , Cross-Sectional Studies , Prospective Studies , Immunophenotyping , Antigen Presentation/immunology , Infectious Disease Transmission, Vertical , Antigens, Bacterial/drug effects