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China Journal of Chinese Materia Medica ; (24): 5398-5404, 2019.
Article in Chinese | WPRIM | ID: wpr-1008412


The study aimed to investigate the effect of processing on lectin protein in four toxic Chinese medicines tubers of Pinellia ternata,P. pedatisecta,Arisema heterophyllum and Typhonium giganteum. Western blot was used to semi-quantitatively analyze the content of lectin in the four kinds of toxic Chinese medicines and their different processed products. Raw products and lectin were treated by heating or soaking in ginger juice or alum solution. The effects of different excipients and the heating methods on lectin proteins were investigated. The results showed that the content of lectin in raw products of P. pedatisecta,P. ternata,A. heterophyllum,and T. giganteum were 7. 3%,4. 9%,2. 7%,2. 3%,respectively. And the content of lectin in Pinelliae Rhizoma praeparatum cum alumine was 0. 027%. Lectin was not detected in the Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine,Arisaematis Rhizma Praeparatum and Typhonii Rhizoma Praeparatum,which indicated that processing could significantly reduce the content of active lectin in raw products. The results also showed that with the prolongation of soaking and heating time,the content of lectin in raw products decreased gradually,while the content was almost unchanged when soaked in ginger juice alone. The effects of different excipients and heating on lectin were the same as those on raw products. Therefore,the method with alum soaking and heating can reduce the content of active lectin,which is the key to reduce the toxicity of toxic Chinese medicines. In this paper,Western blot was used to study the content of toxic protein in Araceae toxic Chinese medicines as an evaluation method of the processing degree.

Araceae/chemistry , Chemistry, Pharmaceutical/methods , Drugs, Chinese Herbal/analysis , Lectins/analysis , Plant Tubers/chemistry , Rhizome/chemistry
Indian J Exp Biol ; 2014 Feb; 52(2): 139-146
Article in English | IMSEAR | ID: sea-150342


The antidiarrhoeal activity of Cryptocoryne spiralis rhizomes extract (250, 500, 750 mg/kg, po) was evaluated using faecal excretion, castor oil-induced diarrhoea, small intestinal transit, intestinal fluid accumulation, gastric emptying and PGE2 induced enteropooling models in rats. In addition, various biochemical estimations, histopathological studies and antibacterial evaluations on strains responsible for diarrhoea were also performed. The results illustrated a significant reduction in normal faecal output rate after 5th and 7th h of treatment, while castor oil-induced diarrhoea model depicted a protection of 55.44% at same dose level from diarrhoea. The other models except, gastric emptying test demonstrated more pronounced effect at same dose level. A significant inhibition in nitric oxide, increase in carbohydrates, protein, DNA, Na+ and K+ level with minimum degeneration of colonic fibrous tissues and potent antibacterial activity were also observed. The antidiarrhoeal potential of C. spiralis may be as a result of antimotility and antisecretory type effect mediated through nitric oxide pathway.

Animals , Antidiarrheals/administration & dosage , Antidiarrheals/chemistry , Araceae/chemistry , Castor Oil/toxicity , Diarrhea/chemically induced , Diarrhea/drug therapy , Diarrhea/pathology , Humans , Metabolic Networks and Pathways/drug effects , Nitric Oxide/metabolism , Plant Extracts/administration & dosage , Plant Extracts/chemistry , Rats , Rhizome/chemistry
Rev. colomb. biotecnol ; 13(1): 94-102, jul. 2011. tab
Article in Spanish | LILACS | ID: lil-600579


El ocumo (Xanthosoma sagittifollium (L.) Schott) es una Arácea cultivada en países tropicales debido al valor nutritivo de sus cormos. La principal limitante para su cultivo es la carencia de semilla de calidad, por esta razón se planteó evaluar la multiplicación de brotes de ocumo blanco en sistemas de inmersión temporal, y el enraizamiento ex vitro de los mismos, para lo cual se estudió el tiempo y la frecuencia de inmersión, y la densidad de explantes sobre la proliferación de los brotes. Asimismo, el efecto del ácido indolacético (AIA) y ácido indolbutírico (AIB) sobre el enraizamiento ex vitro de brotes. De acuerdo con los resultados obtenidos, la mayor eficiencia en la proliferación de brotes se obtuvo utilizando el sistema de inmersión temporal del tipo RITA®, con una frecuencia y tiempo de inmersión de 6 veces/día y 5 min, respectivamente, y una densidad de 9 explantes/RITA®. En el enraizamiento ex vitro se determinó que bajo las condiciones de cultivo empleadas no es necesario el uso de auxinas. Se concluye que es posible la multiplicación eficiente de ocumo blanco en sistemas de inmersión temporal, y realizar el enraizamiento ex vitro sin el uso de auxinas.

The white cocoyam (Xanthosoma sagittifollium (L.) Schott), is an Arácea cultivated in tropical countries, due to the nutritional value of its corms. The main limiting factor for cultivation is the lack of healthy seed, by this reason be outlined to evaluate the multiplication of shoots of white cocoyam in temporary immersion systems and the ex vitro rooting of the same. For that which, itself study, the time and frequency of immersion and the density of explants on the proliferation of the shoots. As well as, the effect of the indole acetic acid (IAA) and indole butyric acid (IBA) on ex vitro rooting the shoots was studied. According to the results obtained, the greater efficiency in the proliferation of shoots was obtained utilizing the temporary immersion system of the type RITA®, with a frequency and time of immersion of 6 times/day and 5 min, respectively and a density of 9 explantes/RITA®. In the ex vitro rooting was determined that under the conditions of employed cultivation is not necessary the use of auxins. It is concluded that is possible the efficient multiplication of white cocoyam in temporary immersion systems and to carry out the ex vitro rooting without the use of auxins.

Araceae/growth & development , Araceae/adverse effects , Araceae/enzymology , Araceae/physiology , Araceae/genetics , Araceae/immunology , Araceae/microbiology , Araceae/parasitology , Araceae/chemistry
Acta amaz ; 40(4): 729-736, dez. 2010. tab
Article in Portuguese | LILACS, VETINDEX | ID: lil-570429


Montrichardia linifera (Araceae), conhecida popularmente como 'aninga', faz parte dos ecossistemas de várzea da Amazônia e da dieta natural de animais como peixe-boi, tartarugas, peixes, búfalo e gado. Com o objetivo de contribuir para o conhecimento químico e valor nutricional da mesma, folhas e frutos de M. linifera foram coletados às margens dos rios Guamá e Maratauíra, no Estado do Pará, Brasil. Em folhas e frutos foram realizadas análises de umidade, resíduo mineral fixo (cinzas), lipídios, proteínas, fibra bruta, concentração de carboidratos e valor calórico. A composição mineral (Ca, Mg, Cu, Fe, Zn e Mn) foi obtida por espectrometria de absorção atômica de chama. Observou-se que tanto as folhas quanto os frutos da aninga, apesar de calóricos (289,75 kcal e 355,12 kcal, respectivamente), possuem baixo valor protéico (0,44% e 0,24 %, respectivamente). As concentrações de manganês obtidas (folha = 3279,46 mg kg-1e fruto = 18151,53 mg kg-1) foram consideradas tóxicas, extrapolando o limite máximo tolerável para ruminantes (1000 mg kg-1). A M. linifera, tem capacidade de absorver e bioacumular grandes quantidades de Ca, Mg e Mn presentes no solo, o que torna inadequada a sua utilização exclusiva na alimentação de quelônios, bovinos e bubalinos, havendo necessidade de mais estudos para sua aplicação como parte da ração.

The aninga (Montrichardia linifera, Araceae) is often found in the floodplain ecosystems of the Amazon and is the natural diet of animals such as manatees, turtles, fish, buffalo and cattle. Aiming to contribute to the chemical knowledge and nutritional value of this plant, leaves and fruits of M. linifera were collected on the banks of the Guama and Maratauira rivers, Para State, Brazil. We determined the moisture content, ash mineral composition, lipids, protein, fiber, carbohydrate and caloric value of the fruits and leaves. The mineral composition (Ca, Mg, Cu, Fe, Zn and Mn) was obtained by flame atomic absorption spectrometry. The leaves and the fruits of M. linifera had caloric values of 289.75 kcal and 355.12 kcal, respectively; and a low protein concentration, 0.44% for leaves and 0.24% for fruits. Manganese concentrations were 3279.46 mg kg-1 for leaves and 18151.53 mg kg-1 for fruits. These Mn concentrations are considered toxic, as they exceed the maximum tolerable for the ruminants (1000 mg kg-1). The M. linifera has the capacity to absorb and bioaccumulate large amounts of Ca, Mg and Mn in the soil, which makes it inappropriate for exclusive use as food for turtles, cattle and buffaloes, requiring more studies for its application as part of the diet.

Plant Leaves/chemistry , Araceae/chemistry , Fruit/chemistry , Nutritive Value , Ruminants , Amazonian Ecosystem
Arq. neuropsiquiatr ; 65(3b): 822-825, set. 2007. ilus, tab
Article in English | LILACS | ID: lil-465187


BACKGROUND: Trigeminal sensory neuropathy (TSN) describes a heterogeneous group of disorders manifesting as facial numbness. OBJECTIVE: We report the case of a patient who had TSN associated with contact dermatitis due to Anthurium sp. METHOD/RESULTS: A 21-year-old female patient developed left hemifacial contact dermatitis after exposure to the anthurium plant. The patient had paresthesias and pain in the V2 and V3 divisions of the left trigeminal nerve. Eight days after its onset the dermatitis resolved, but numbness developed in the V2 and V3 divisions of the left trigeminal nerve. Cranial CT scan and MRI, as well as CSF and extensive work-up exams, were normal. After one month the symptoms disappeared completely. CONCLUSION: Anthurium sp, an indoor ornamental plant that contains calcium oxalate crystals, and can causes contact dermatitis. To our knowledge, this is the first report associating TSN with contact dermatitis due to Anthurium sp.

INTRODUÇÃO: A neuropatia trigeminal sensitiva (NTS) representa um grupo heterogêneo de doenças, cuja manifestação clínica é a presença de dormência na região facial. OBJETIVO: Relatamos o caso de paciente que apresenta NTS associada com dermatite de contato (DC) devido à planta Anthurium sp. MÉTODO/RESULTADOS: Uma paciente com 21 anos desenvolveu DC na região hemi-facial esquerda, após exposição à planta Anthurium sp. Após a resolução do quadro de dermatite, a referida paciente apresentou dormência e parestesias no território do segundo e terceiro ramos do nervo trigêmeo esquerdo. Um mês após o início do quadro houve resolução completa dos sintomas. CONCLUSÃO: O Anthurium é uma planta ornamental que contém cristas de oxalato de cálcio, que podem causar DC. Para o nosso conhecimento este é o primeiro relato associando NTS e dermatite de contato devido à exposição ao Anthurium sp.

Adult , Female , Humans , Araceae/adverse effects , Dermatitis, Allergic Contact/etiology , Facial Dermatoses/etiology , Hypesthesia/etiology , Trigeminal Nerve Diseases/etiology , Analgesics, Non-Narcotic/therapeutic use , Araceae/chemistry , Carbamazepine/therapeutic use , Dermatitis, Allergic Contact/diagnosis , Dermatitis, Allergic Contact/drug therapy , Facial Dermatoses/diagnosis , Facial Dermatoses/drug therapy , Hypesthesia/diagnosis , Hypesthesia/drug therapy , Trigeminal Nerve Diseases/diagnosis , Trigeminal Nerve Diseases/drug therapy , Trigeminal Neuralgia/diagnosis , Trigeminal Neuralgia/drug therapy , Trigeminal Neuralgia/etiology
Indian J Exp Biol ; 2006 May; 44(5): 422-4
Article in English | IMSEAR | ID: sea-61260


Analgesic activity of methanol leaf extract of C. scandens obtained by column chromatography and its graded solvent fractions, was evaluated in mice using acetic acid-induced abdominal writhing and formalin-induced paw licking. The extract and fractions significantly inhibited abdominal writhing and two phases of formalin-induced paw licking in mice, indicating that antinociceptive activity may involve inhibition of pain by peripheral and central mechanisms.

Analgesics/pharmacology , Animals , Araceae/chemistry , Female , Male , Mice , Plant Extracts/pharmacology , Plant Leaves/chemistry