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1.
Article in English | WPRIM | ID: wpr-929242

ABSTRACT

Wantong Jingu Tablet (WJT), a mixture of traditional Chinese medicine, was reported to relieve the symptoms of rheumatoid arthritis (RA), but its pharmacological mechanism was not completely understood. The aim of this study was to investigate the therapeutic mechanisms of WJT for RA in vivo. The effects of WJT on joint pathology, as well as the levels of Bax, Bcl-2, caspase-3, cleaved-caspase-3, ERK1/2, pERK1/2, TNF-α, IL-1β, and IL-6 were measured using collagen-induced arthritis (CIA) rats. The intestinal flora composition and the metabolites alteration were analyzed by 16S rDNA sequencing and metabolomics method, respectively. We found that WJT ameliorated the severity of the CIA rats which might be mediated by inducing apoptosis, inactivating the MEK/ERK signals and reducing the production of pro-inflammatory cytokines. WJT, in part, relieved the gut microbiota dysbiosis, especially bacterial phylum Bacteroidetes, Tenericutes and Deferribacteres, as well as bacterial genus Vibrio, Macrococcus and Vagococcus. 3'-N-debenzoyl-2'-deoxytaxol, tubulysin B, and magnoline were significantly associated with the specific genera. We identified serotonin, glutathione disulfide, N-acetylneuraminic acid, naphthalene and thromboxane B2 as targeted molecules via metabolomics. Our findings contributed to the understanding of RA pathogenesis, and WJT played essential roles in gut microbiota health and metabolite modulation in the CIA rats.


Subject(s)
Animals , Arthritis, Experimental/drug therapy , Arthritis, Rheumatoid/drug therapy , Dysbiosis , Metabolomics , Rats , Tablets
2.
Article in Chinese | WPRIM | ID: wpr-880777

ABSTRACT

OBJECTIVE@#To explore the mechanism of @*METHODS@#Healthy male DBA/1 mice were used for CIA modeling. Twenty-five CIA mice with successful modeling and similar arthritis index (AI) scores were randomized equally into model group (CIA), methotrexate (MTX) group, and low-, medium-, and high-dose XWGD groups (0.975, 1.95, and 3.9 g/mL, respectively), with another 5 normal mice as the normal control group. The mice in normal control and CIA groups were given saline once a day, those in MTX group were given 0.1 mg/mL MTX once a week, and those in XWGD groups were treated daily via garage of XWGD containing crude drugs of different doses for 28 consecutive days. The AI score and HE staining were used to evaluate the changes in the joints of the CIA mice. The effect of XWGD on Th1, Th17, MDSC, G-MDSC and M-MDSC cells were evaluated with flow cytometry.@*RESULTS@#Treatment with MTX and different doses of XWGD significantly decreased the AI score of the mice and relieved joint inflammation as compared with the model group (@*CONCLUSIONS@#XWGD can improve joint inflammation in CIA mice by increasing the percentages of G-MDSC cells and decreasing the percentages of M-MDSC, Th1 and Th17 cells, and a high dose of XWGD can produce an equivalent therapeutic effect to methotrexate but with better safety.


Subject(s)
Animals , Arthritis, Experimental/drug therapy , Arthritis, Rheumatoid/drug therapy , Male , Methotrexate , Mice , Mice, Inbred DBA , Th17 Cells
3.
Braz. j. med. biol. res ; 53(6): e9489, 2020. graf
Article in English | LILACS, ColecionaSUS | ID: biblio-1132521

ABSTRACT

Rheumatoid arthritis (RA) is an autoimmune disease of knee joints involving pain and inflammation. Rhoifolin is a plant flavonoid known to have antioxidant and anti-inflammatory properties. This study was taken to identify the effect of rhoifolin on complete Freund's adjuvant (CFA)-induced arthritis in the rat model. Treatment with rhoifolin (10 and 20 mg/kg) showed a significant improvement in the overall health parameters such as paw edema and weight loss. This improvement in morphological parameters corroborated the findings with gross morphological changes observed in the histopathological analysis. Rhoifolin treatment also caused a significant decrease in oxidative stress, evident from changes in intracellular levels of glutathione, glutathione peroxidase, malondialdehyde, and superoxide dismutase in the articular cartilage tissue. Moreover, proinflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin(IL)-1β, and IL-6 showed a significant downregulation of gene expression and intracellular protein concentration levels. The NF-κB pathway showed a significant attenuation as evident in the significant reduction in the levels of NF-κB p65 and p-IκB-α. These results indicated that rhoifolin can be a natural therapeutic alternative to the extant regimens, which include non-steroidal anti-inflammatory drugs and immunosuppressants. Additionally, the antioxidant and anti-inflammatory action of rhoifolin was probably mediated by the NF-κB pathway. However, the exact target molecules of this pathway need to be determined in further studies.


Subject(s)
Animals , Male , Rats , Arthritis, Experimental/drug therapy , Arthritis, Rheumatoid/drug therapy , Flavonoids/administration & dosage , Freund's Adjuvant/administration & dosage , Cytokines/blood , Oxidative Stress/drug effects , Disaccharides/administration & dosage , Arthritis, Experimental/metabolism , Arthritis, Rheumatoid/metabolism , Biomarkers/blood , NF-kappa B/drug effects , NF-kappa B/metabolism , Interleukin-6/blood , Tumor Necrosis Factor-alpha/blood , Interleukin-1beta/blood , Glycosides/administration & dosage
4.
Braz. j. med. biol. res ; 51(1): e6799, 2018. tab, graf
Article in English | LILACS | ID: biblio-889013

ABSTRACT

Arthritis is positively associated with the decline of sex hormones, especially estrogen. Tamoxifen (TMX) is a selective estrogen receptor modulator, possessing agonist or antagonistic activity in different tissues. Thus, the objective of this study was to investigate the effect of TMX on the zymosan-induced arthritis model. Female Swiss normal and ovariectomized (OVX) mice were divided into groups and treated for five days with TMX (0.3, 0.9 or 2.7 mg/kg) or 17-β-estradiol (E2, 50 µg/kg). On the fifth day, arthritis was induced and 4 h later, leukocyte migration into joint cavities was evaluated. The neutrophil migration in OVX animals, but not in normal mice, treated with TMX (all tested doses) was significantly decreased compared with mice that received the vehicle (P≤0.05). Similarly, this effect was also demonstrated in the E2-treated group. Therefore, the present study demonstrates that TMX presented agonist effects in inhibiting neutrophil migration and preventing arthritis progression in OVX mice.


Subject(s)
Animals , Female , Rabbits , Arthritis, Experimental/drug therapy , Tamoxifen/pharmacology , Ovariectomy , Selective Estrogen Receptor Modulators/pharmacology , Organ Size/drug effects , Time Factors , Uterus/drug effects , Zymosan , Cell Movement/drug effects , Treatment Outcome , Estrous Cycle/drug effects , Disease Models, Animal , Estrogen Antagonists/pharmacology , Cell Migration Assays, Leukocyte , Neutrophils/drug effects
5.
Braz. j. med. biol. res ; 48(10): 863-870, Oct. 2015. tab, ilus
Article in English | LILACS | ID: lil-761606

ABSTRACT

We aimed to investigate the effects of an anti-tumor necrosis factor-α antibody (ATNF) on cartilage and subchondral bone in a rat model of osteoarthritis. Twenty-four rats were randomly divided into three groups: sham-operated group (n=8); anterior cruciate ligament transection (ACLT)+normal saline (NS) group (n=8); and ACLT+ATNF group (n=8). The rats in the ACLT+ATNF group received subcutaneous injections of ATNF (20 μg/kg) for 12 weeks, while those in the ACLT+NS group received NS at the same dose for 12 weeks. All rats were euthanized at 12 weeks after surgery and specimens from the affected knees were harvested. Hematoxylin and eosin staining, Masson's trichrome staining, and Mankin score assessment were carried out to evaluate the cartilage status and cartilage matrix degradation. Matrix metalloproteinase (MMP)-13 immunohistochemistry was performed to assess the cartilage molecular metabolism. Bone histomorphometry was used to observe the subchondral trabecular microstructure. Compared with the rats in the ACLT+NS group, histological and Mankin score analyses showed that ATNF treatment reduced the severity of the cartilage lesions and led to a lower Mankin score. Immunohistochemical and histomorphometric analyses revealed that ATNF treatment reduced the ACLT-induced destruction of the subchondral trabecular microstructure, and decreased MMP-13 expression. ATNF treatment may delay degradation of the extracellular matrix via a decrease in MMP-13 expression. ATNF treatment probably protects articular cartilage by improving the structure of the subchondral bone and reducing the degradation of the cartilage matrix.


Subject(s)
Animals , Female , Adalimumab/pharmacology , Antirheumatic Agents/pharmacology , Bone and Bones/drug effects , Cartilage, Articular/drug effects , Osteoarthritis/drug therapy , Tumor Necrosis Factor-alpha/antagonists & inhibitors , Arthroplasty, Subchondral , Anterior Cruciate Ligament/surgery , Arthritis, Experimental/drug therapy , Bone and Bones/metabolism , Cartilage, Articular/metabolism , Extracellular Matrix/drug effects , Hindlimb/pathology , Hindlimb/surgery , Immunohistochemistry , Injury Severity Score , /drug effects , /metabolism , Osteoarthritis/surgery , Protective Factors , Random Allocation , Rats, Sprague-Dawley
6.
Säo Paulo med. j ; 133(1): 4-12, Jan-Fev/2015. tab, graf
Article in English | LILACS | ID: lil-733011

ABSTRACT

CONTEXT AND OBJECTIVE: The development of a slow and progressive mechanical model for osteoarthritis is important for correlation with clinical practice, and for evaluating the effects of disease-modifying medications. A mechanical osteoarthritis model was developed to evaluate the effects of intra-articular hyaluronic acid (HA) injection and oral diacerein administration. DESIGN AND SETTING: Experimental study at the Department of Orthopedics and Traumatology, Universidade de São Paulo. METHOD: Total medial meniscectomy was performed on seven groups of ten Wistar rats each, comprising four control groups (C) and three study groups (S). C.I: operated, non-medicated; C.II: operated, injections of HA vehicle; C.III: non-operated, non-medicated; C.IV: operated, non-medicated, sacrificed three months post-meniscectomy; S.I: operated, receiving intra-articular HA injections; S.II: operated, oral diacerein from the third to the seventh postoperative month; S.III: operated, received both medications. All the animals (except C.IV) were sacrificed seven months post-meniscectomy. All femurs and tibias were assessed histologically. RESULTS: The most severe degenerative histological changes were in the tibias of the operated knees. On the contralateral side, all groups had mild changes on the tibial surface. The femoral surface had ...


CONTEXTO E OBJETIVO: Desenvolver um modelo osteoartrítico mecânico lento e progressivo é importante para correlação com a prática clínica e para avaliar os efeitos de medicamentos modificadores da doença. Um modelo mecânico de osteartrite foi desenvolvido para avaliar os efeitos de injeção intra-articular de hialuronato de sódio (AH) e de administração de diacereína oral. DESENHO E LOCAL: Estudo experimental no Departamento de Ortopedia e Traumatologia, Universidade de São Paulo. MÉTODO: Meniscectomia medial total foi feita em sete grupos de dez ratos Wistar, sendo quatro grupos controle (C) e três grupos estudo (E). C.I: operado, não medicado; C.II: operado, recebendo injeções do veículo do AH; C.III: não operado, não medicado; C.IV: operado, não medicado, sacrificado três meses pósmeniscectomia; EI: operado, recebendo injeções de AH intra-articular; E.II: operado, recebendo diacereína oral do terceiro ao sétimo mês pós-operatório; E.III: operado, recebeu ambas medicações. Todos os animais (exceto C.IV) foram sacrificados sete meses pós-meniscectomia. Todos os fêmures e tíbias foram analisados histologicamente. RESULTADOS: As alterações histológicas degenerativas ...


Subject(s)
Animals , Male , Anthraquinones/administration & dosage , Anti-Inflammatory Agents/administration & dosage , Arthritis, Experimental/drug therapy , Hyaluronic Acid/administration & dosage , Menisci, Tibial/surgery , Osteoarthritis, Knee/drug therapy , Viscosupplements/administration & dosage , Administration, Oral , Arthritis, Experimental/etiology , Arthritis, Experimental/pathology , Disease Models, Animal , Disease Progression , Drug Therapy, Combination , Injections, Intra-Articular , Osteoarthritis, Knee/etiology , Osteoarthritis, Knee/pathology , Random Allocation , Rats, Wistar , Severity of Illness Index
7.
Acta cir. bras ; 29(11): 727-734, 11/2014. tab, graf
Article in English | LILACS | ID: lil-728645

ABSTRACT

PURPOSE: To evaluate the effect of curcumin in the acute phase of zymosan-induced arthritis. METHODS: Twenty-eight male rats were subjected to intra-articular infiltration of zymosan of both knees and, in four the infiltration was made with saline. The animals were divided into five groups second received every six hours by gavage: corn oil by (positive and negative control); curcumin (100 mg/kg); prednisone 1 mg/kg/day; prednisone 8 mg/kg. All animals were sacrificed after six, 12, 24 and 48 hours of the infiltration. The knees were removed for evaluation of neutrophil infiltration. The number of neutrophils was counted by computer-assisted analysis of the images. The neutrophil infiltrate was stratified into four grades: 0 = normal; + = mild; ++/+++ = moderate; > ++++ = severe. The results were compared using the Mann-Whitney test and the variance by Kruskal-Wallis test adopting a significance level of 5% (p<0.05). RESULTS: Curcumin reduces inflammatory activity in the first six hours after zymosan-induced arthritis when compared to saline (p<0.01). This was also observed in animals subjected to administration of prednisone (1 mg/kg) and those treated with prednisone (8 mg/kg). Curcumin was more effective than lower doses of prednisone in the first six hours after induction of the arthritis. After 12, 24 and 48 hours, curcumin does not have the same anti-inflammatory effects when compared to prednisone. After 48 hours, prednisone is more effective than curcumin in reducing the inflammatory infiltrate regardless of the dose of prednisone used. CONCLUSION: Oral administration of curcumin reduces inflammation in the first six hours after experimentally zymosan-induced arthritis. .


Subject(s)
Animals , Male , Anti-Inflammatory Agents, Non-Steroidal/administration & dosage , Arthritis, Experimental/drug therapy , Curcumin/administration & dosage , Neutrophil Infiltration/drug effects , Administration, Oral , Arthritis, Experimental/chemically induced , Arthritis, Experimental/pathology , Disease Models, Animal , Neutrophils/drug effects , Prednisolone/administration & dosage , Rats, Wistar , Reproducibility of Results , Severity of Illness Index , Time Factors , Treatment Outcome , Zymosan
8.
Indian J Exp Biol ; 2014 Aug; 52(8): 763-772
Article in English | IMSEAR | ID: sea-153757

ABSTRACT

Nanoscience and Nanotechnology have found their way in the fields of pharmacology and medicine. The conjugation of drug to nanoparticles combines the properties of both. In this study, gold nanoparticle (GNP) was conjugated with NKCT1, a cytotoxic protein toxin from Indian cobra venom for evaluation of anti-arthritic activity and toxicity in experimental animal models. GNP conjugated NKCT1 (GNP-NKCT1) synthesized by NaBH4 reduction method was stable at room temperature (25±2 °C), pH 7.2. Hydrodynamic size of GNP-NKCT1 was 68–122 nm. Arthritis was developed by Freund's complete adjuvant induction in male albino rats and treatment was done with NKCT1/GNP-NKCT1/standard drug. The paw/ankle swelling, urinary markers, serum markers and cytokines were changed significantly in arthritic control rats which were restored after GNP-NKCT1 treatment. Acute toxicity study revealed that GNP conjugation increased the minimum lethal dose value of NKCT1 and partially reduced the NKCT1 induced increase of the serum biochemical tissue injury markers. Histopathological study showed partial restoration of toxic effect in kidney tissue after GNP conjugation. Normal lymphocyte count in culture was in the order of GNP-NKCT1>NKCT1>Indomethacine treatment. The present study confirmed that GNP conjugation increased the antiarthritic activity and decreased toxicity profile of NKCT1.


Subject(s)
Animals , Arthritis, Experimental/drug therapy , Arthritis, Experimental/pathology , Edema/drug therapy , Edema/pathology , Elapid Venoms/administration & dosage , Elapid Venoms/chemistry , Elapidae , Gold/administration & dosage , Gold/chemistry , Humans , Lymphocyte Count , Metal Nanoparticles/administration & dosage , Metal Nanoparticles/chemistry , Mice , Rats
9.
Indian J Exp Biol ; 2014 Mar; 52(3): 215-222
Article in English | IMSEAR | ID: sea-150351

ABSTRACT

Cynodon dactylon (L.) (Poaceae) is traditionally used herb to treat fevers, skin diseases and rheumatic affections. The ethanolic extract of C. dactylon was found to be safe at all the dose levels (100, 200 and 400 mg/kg, orally) and there was no mortality up to the dose of 5000 mg/kg of extract when administered orally. C. dactylon showed significant antiarthritic activity against Freund’s complete adjuvant induced arthritis in rats. Treatment with C. dactylon significantly reduced the mean percentage change in injected and non injected paw, ankle diameter, clinical severity and significantly increased body weight. Results were confirmed using biochemical parameters; there was a significant improvement in the levels of Hb and RBC in C. dactylon treated rats. The increased levels of WBC, ESR, C- reactive protein (CRP) and TNFα were significantly suppressed in C. dactylon treated rats. C. dactylon showed protective effect in arthritic joints but it has been supported by an improvement in bone lesions rather than in cartilage lesions. It can be concluded that ethanolic extract of C. dactylon at a dose of 400 mg/kg is effective in improving haematological level, CRP and reducing TNFα level. Phytochemical screening showed the presence of alkaloids, flavonoids and glycosides in ethanolic extract. All the above results support the traditional uses of the plant in the treatment of rheumatoid arthritis.


Subject(s)
Animals , Anti-Arrhythmia Agents/administration & dosage , Anti-Arrhythmia Agents/chemistry , Arthritis, Experimental/chemically induced , Arthritis, Experimental/drug therapy , Arthritis, Experimental/pathology , Blood Cell Count , Blood Cells/drug effects , Blood Cells/metabolism , C-Reactive Protein/metabolism , Cynodon/chemistry , Male , Mice , Plant Extracts/administration & dosage , Plant Extracts/chemistry , Rats , Tumor Necrosis Factor-alpha/blood
10.
Article in English | IMSEAR | ID: sea-163295

ABSTRACT

Aims: The present study was undertaken to explore in vivo antioxidant potential of ethanol extracts of Nyctanthes arbor-tristis leaf and stem in adjuvant induced arthritic rats. Methodology: Arthritis induced rats were administered with extract of Nyctanthes arbortristis leaf and stem. (150 mg/kg body Weight/rat/day for 30 days. Results: A significant decrease in paw edema was observed following oral administration of the leaf and stem extracts. A significant (p<0.05) increase in the level of tissue TBARS, GPx and catalase was seen in arthritis induced rats (group II) and NAT treated rats (group III and group IV) showed a significant decrease in lipid peroxides, GPx and catalase level to near normalcy. The activity of total tissue SOD was found significantly (p<0.05) low in arthritis induced rats (group II) while a substantial increase in the activity to near normal level was noticed in NAT administered rats. The alterations in hematological and other biochemical parameters were restored to near normal levels after a treatment period of 30 days. The structural changes of the tissues shows the therapeutic ability of Nyctanthes arbor-tristis stem and leaf in experimental animals which were further evidenced by histological observations made on the hind limb tissue. Conclusion: As Nyctanthes arbor-tristis is of natural origin, it is a safe and effective intervention for free radical mediated diseases.


Subject(s)
Animals , Anti-Inflammatory Agents/pharmacology , Antioxidants , Arthritis, Experimental/chemically induced , Arthritis, Experimental/drug therapy , Free Radical Scavengers/pharmacology , Free Radicals/metabolism , Lipid Peroxidation , Oleaceae/chemistry , Plant Extracts/isolation & purification , Plant Extracts/therapeutic use , Plant Leaves/chemistry , Rats , Superoxide Dismutase
11.
Article in English | WPRIM | ID: wpr-223714

ABSTRACT

Interleukin (IL)-27 is a novel cytokine of the IL-6/IL-12 family that has been reported to be involved in the pathogenesis of autoimmune diseases and has a pivotal role as both a pro- and anti-inflammatory cytokine. We investigated the in vivo effects of IL-27 on arthritis severity in a murine collagen-induced arthritis (CIA) model and its mechanism of action regarding control of regulatory T (Tregs) and IL-17-producing T helper 17 (Th17) cells. IL-27-Fc-treated CIA mice showed a lower severity of arthritis. IL-17 expression in the spleens was significantly decreased in IL-27-Fc-treated CIA mice compared with that in the CIA model. The Th17 population was decreased in the spleens of IL-27-Fc-treated CIA mice, whereas the CD4+CD25+Foxp3+ Treg population increased. In vitro studies revealed that IL-27 inhibited IL-17 production in murine CD4+ T cells, and the effect was associated with retinoic acid-related orphan receptor gammaT and signal transducer and activator of transcription 3 inhibition. In contrast, fluorescein isothiocyanate-labeled forkhead box P3 (Foxp3) and IL-10 were profoundly augmented by IL-27 treatment. Regarding the suppressive capacity of Treg cells, the proportions of CTLA-4+ (cytotoxic T-lymphocyte antigen 4), PD-1+ (programmed cell death protein 1) and GITR+ (glucocorticoid-induced tumor necrosis factor receptor) Tregs increased in the spleens of IL-27-Fc-treated CIA mice. Furthermore, in vitro differentiated Treg cells with IL-27 exerted a more suppressive capacity on T-cell proliferation. We found that IL-27 acts as a reciprocal regulator of the Th17 and Treg populations in CD4+ cells isolated from healthy human peripheral blood mononuclear cells (PBMCs), as well as from humans with rheumatoid arthritis (RA) PBMCs. Our study suggests that IL-27 has the potential to ameliorate overwhelming inflammation in patients with RA through a reciprocal regulation of Th17 and Treg cells.


Subject(s)
Animals , Arthritis, Experimental/drug therapy , Cells, Cultured , Humans , Interleukins/immunology , Male , Mice , Mice, Inbred C57BL , Mice, Inbred DBA , T-Lymphocytes, Regulatory/immunology , Th17 Cells/immunology
12.
West Indian med. j ; 60(6): 615-621, Dec. 2011. ilus, graf, tab
Article in English | LILACS | ID: lil-672822

ABSTRACT

OBJECTIVE: To investigate the anti-inflammatory effects of a hexane extract of Cassia alata leaves in complete Freund's adjuvant (CFA) arthritis in rats. METHOD: A hexane extract of Cassia alata leaves was administered by oral gavage to CFA arthritic rats (500 mg/kg, n = 6). Controls received corn oil (2 ml, n = 6). The CFA arthritic model was induced by the injection of 0.5 ml (CFA) into the synovial cavity of the right knee joint of the hind leg of rats. The ability of the plant extract to reduce swelling as a sign of arthritic inflammation was assessed by obtaining the circumference of the knee joint before and for twenty eight days post arthritis induction. Reduction of leukocyte infiltration into the blood and synovial cavity of the arthritic rats were assessed using automated counting and Wrights method. Protection against cartilage erosion was also assessed histologically. RESULTS: Cassia alata extract significantly (p = 0.0032) reduced knee circumference (swelling) in the CFA arthritic rats. Total and differential leukocyte counts in both blood and synovial fluid from Cassia alata treated animals were significantly (p < 0.05) lower than in control animals. Protective effects against cartilage degradation on the femoral head of the knee joint were observed in Cassia alata treated animals, as normal cartilage structure and chondrocyte arrangement were maintained. CONCLUSIONS: The results suggest that Cassia alata exhibits anti-inflammatory activities that should be further examined and potentially exploited for anti-arthritic therapies.


OBJETIVO: Investigar los efectos anti-inflamatorios del extracto de hexano de hojas de Cassia alata en artritis inducida por adyuvante completo de Freund (CFA) en ratas. MÉTODO: Un extracto de hexano de hojas de Cassia alata fue administrado por gavage oral a ratas artríticas por CFA (500 mg/kg, n = 6). Los controles recibieron aceite de maíz (2 ml, n = 6). El modelo artrítico de CFA fue inducido inyectando 0.5 ml (CFA) en la cavidad sinovial de la rótula derecha de la pata trasera de las ratas. La capacidad del extracto de la planta en cuanto a reducir la inflamación como signo de la inflamación artrítica, fue evaluada obteniendo la circunferencia de la rótula antes y durante veintiocho días posterior a la inducción de la artritis. La reducción de la infiltración de leucocitos en la sangre y la cavidad sinovial de las ratas artríticas fue evaluada usando el conteo automatizado y el método de Wright. También se evaluó histológicamente la protección contra la erosión del cartílago. RESULTADOS: El extracto de Cassia alata redujo significativamente (p = 0.0032) la circunferencia de la rodilla (inflamación) en las ratas artríticas por CFA. Los conteos totales y diferenciales de leucocitos tanto en la sangre como en el líquido sinovial de los animales tratados con Cassia alata fueron significativamente (p < 0.05) más bajos en los animales del control. Los efectos protectores contra la degradación del cartílago en la cabeza femoral de la rótula fueron observados en los animales tratados con Cassia alata, ya que se mantuvieron la estructura normal del cartílago y las disposición de los condrocitos. CONCLUSIONES: Los resultados sugieren que la Cassia alata exhibe propiedades anti-inflamatorias que deben ser examinadas ulteriormente y explotadas potencialmente para las terapias anti-artríticas.


Subject(s)
Animals , Female , Rats , Arthritis, Experimental/drug therapy , Cassia/chemistry , Freund's Adjuvant , Plant Extracts/pharmacology , Plant Leaves/chemistry , Administration, Oral , Arthritis, Experimental/chemically induced , Knee Joint , Leukocyte Count , Plant Extracts/administration & dosage , Rats, Sprague-Dawley
13.
Article in English | IMSEAR | ID: sea-135772

ABSTRACT

Background & objectives: Majoon Suranjan (MS) is a polyherbal formulation used in Unani system of medicine for the treatment of rheumatoid arthritis (RA). The present study evaluates the antiarthritic efficacy of this formulation in three different experimental models. Methods: The anti-inflammatory activity of MS (in doses of 450, 900 and 1800 mg/kg body wt) was evaluated using the turpentine oil induced paw oedema model and the antiarthritic efficacy was evaluated using the formaldehyde and complete Freund's adjuvant (CFA) induced arthritis models. Aspirin (100 mg/kg body wt) was used as the standard drug in all the models. In order to assess the safety of the test drug, oral acute and 28 day toxicity studies were also carried out. Results: MS produced a dose dependent protective effect in all the experimental models. Its antiarthritic efficacy was comparable to aspirin in formaldehyde induced arthritis and was superior to aspirin in turpentine oil induced paw oedema and CFA induced arthritis. MS also inhibited the delayed increase in joint diameter as seen in control and aspirin treated animals in CFA induced arthritis. Oral LD50 of MS was found to be >5000 mg/kg in rats. Chronic administration did not produce any significant physiological changes in the tested animals. Interpretation & conclusions: Results of the present study suggest that the antiarthritic activity of MS was due to the interplay between its anti-inflammatory and disease modifying activities, thus supporting its use in traditional medicine for the treatment of RA.


Subject(s)
Analysis of Variance , Animals , Arthritis, Experimental/drug therapy , Aspirin/administration & dosage , Aspirin/pharmacology , Dose-Response Relationship, Drug , Formaldehyde , Male , Medicine, Unani , Phytotherapy/methods , Plant Extracts/pharmacology , Plant Extracts/toxicity , Rats , Rats, Wistar , Toxicity Tests , Turpentine
14.
Clinics ; 64(4): 357-362, 2009. graf, tab, ilus
Article in English | LILACS | ID: lil-511939

ABSTRACT

OBJECTIVE: To evaluate the anti-arthritic potential of the plant Justicia gendarussa using two different rat models. MATERIALS AND METHOD: The anti-arthritic potential of the alcoholic extract of the plant Justicia gendarussa was evaluated using the Freund's adjuvant-induced and collagen-induced arthritic rat models. The rats were treated with the ethanolic extract of Justicia gendarussa and with standard aspirin. RESULTS: The ethanolic extract of Justicia gendarussa showed significant anti-arthritic activity that was statistically similar to that of aspirin. Our results suggest that the alcoholic extract of Justicia gendarussa exhibits significant anti-arthritic potential.


Subject(s)
Animals , Cattle , Rats , Acanthaceae , Arthritis, Experimental/drug therapy , Phytotherapy , Plant Extracts/therapeutic use , Adjuvants, Immunologic , Acanthaceae/adverse effects , Arthritis, Experimental/chemically induced , Collagen Type II , Drug Evaluation, Preclinical , Freund's Adjuvant , Plant Extracts/adverse effects , Rats, Wistar
15.
Article in English | IMSEAR | ID: sea-46802

ABSTRACT

To evaluate the effect of Calotropis G in various experimental animal models. The anti-inflammatory activity was evaluated using carrageenin-induced kaolin -induced rat paw oedema for acute and cotton-pellet granuloma, adjuvant-induced arthritis model for chronic inflammation. Antipyretic activity was carried out using yeast induced pyresis method. Phenylquinone--induced writhing method in mice was used for analgesic activity. Test compounds exhibited variable anti-inflammatory activity and peak activity of the test compounds were reached at 2 h. Alkaloid fraction possesses comparatively high initial anti-inflammatory activity. The residual anti-inflammatory activity of alkaloid fraction of Calotropis G suggest either a greater protein binding nature of the compound there by providing a slow released pool of active drug molecule in the system or non available of possible bioactive metabolites to retain the activity profile relation.


Subject(s)
Animals , Anti-Inflammatory Agents/pharmacology , Arthritis, Experimental/drug therapy , Calotropis , Chronic Disease , Edema/drug therapy , Homeopathy , Inflammation/drug therapy , Male , Mice , Models, Animal , Phenylbutazone/pharmacology , Plant Extracts/pharmacology , Rats
16.
Indian J Exp Biol ; 2006 May; 44(5): 403-7
Article in English | IMSEAR | ID: sea-62319

ABSTRACT

In complete freund's adjuvant induced arthritis in male albino rats, a significant increase in serum lipid peroxidase besides increase in paw swelling and a significant decrease in superoxide dismutase, glutathione peroxidase and total reduced glutathione levels were observed. Arthritin produced a marked reversal of these enzyme levels, besides a significant reduction in paw swelling. The results suggest that, the polyherbal formulation 'Arthritin' exerts its effects by modulating lipid peroxidation and enhancing anti-oxidant and detoxifying enzyme systems.


Subject(s)
Animals , Antioxidants/pharmacology , Arthritis, Experimental/drug therapy , Herbal Medicine , Male , Plant Extracts/pharmacology , Rats , Rats, Wistar
17.
Indian J Exp Biol ; 2005 Jan; 43(1): 46-52
Article in English | IMSEAR | ID: sea-59742

ABSTRACT

Chronobiology of rheumatoid arthritis (RA) was studied using a standard adjuvant arthritis animal model. Chronopharmacology of ketoprofen, and its solid dispersion forms was also studied. Temporal variations in the degree of articular inflammation (paw volume) and progression of articular destruction were studied by injecting Freund's Complete Adjuvant (FCA) at 0800 and 2000 hrs. Temporal variations in anti-inflammatory effects and ulcerogenic effect were also studied by administration of plain ketoprofen (20 mg/kg) and its solid dispersion with hydroxypropyl beta-cyclodextrin (equivalent to 20 mg/kg of ketoprofen) at the same time points (0800 and 2000 hrs) twice weekly for 22 days. Solid dispersion of ketoprofen was found to be more effective in inhibiting progression of RA. The incidence and severity of ulcers was found to be less with the solid dispersion. The protective effect of ketoprofen and its solid dispersion was significantly higher when these were administered at 0800 hrs. The incidence of ulceration was more in 2000 hrs group. Thus, it was observed that in the adjuvant induced arthritis model, inflammation and articular damage was significantly greater in the rest period of diurnally active rats than in the activity phase. KPF and its solid dispersion showed better protection from inflammation in the morning than in the evening.


Subject(s)
Administration, Oral , Animals , Arthritis, Experimental/drug therapy , Chronotherapy/methods , Circadian Rhythm/physiology , Excipients , Female , Ketoprofen/administration & dosage , Male , Powders , Rats , Solubility , Stomach Ulcer/chemically induced , beta-Cyclodextrins
18.
Indian J Exp Biol ; 2003 Aug; 41(8): 890-4
Article in English | IMSEAR | ID: sea-61211

ABSTRACT

Column chromatographic fractionation of essential oil obtained by hydrodistillation from the flowering tops of S. ixiocephala resulted in the isolation of beta-caryophyllene, fenchyl acetate, T-cadinol and a new sesquiterpene alcohol for which a name ixiocephol has been proposed. The beta-caryophyllene and fenchyl acetate were identified by Co-TLC with authentic samples whereas T-cadinol and ixiocephol were structurally elucidated by UV, IR, 1H NMR, 13C NMR and Mass spectral data. The GC-MS analysis of the essential oil has also revealed the presence of various monoterpenoids and sesquiterpenoids. The essential oil of S. ixiocephala demonstrated a dose dependant anti-inflammatory activity in carrageenan-induced rat paw oedema. It has also revealed good activity in cotton pellet granuloma and adjuvant induced arthritis model in rats.


Subject(s)
Acanthaceae/chemistry , Animals , Anti-Inflammatory Agents, Non-Steroidal/isolation & purification , Arthritis, Experimental/drug therapy , Disease Models, Animal , Dose-Response Relationship, Drug , Female , Gas Chromatography-Mass Spectrometry , Granuloma, Foreign-Body/drug therapy , Male , Oils, Volatile/isolation & purification , Phytotherapy , Rats , Rats, Wistar
19.
Article in English | WPRIM | ID: wpr-148804

ABSTRACT

In this study, we aimed to determine the antinociceptive and/or anti-inflammatory effect of Bang-Poong (BP, Radix Ledebouriellae) on Freund's adjuvant-induced arthritis in rats. Traditionally, BP has been used to treat several inflammatory diseases such as arthritis. Whole BP is extracted into two fractions that were ethylacetate and hexane-soluble fractions. Adult Sprague-Dawley rats (n=30, 130-150 g) were subcutaneously administered by the Freund's complete adjuvant (FCA) into the plantar surface of right hindpaw. Twelve days after the injection of FCA, the rats initially showed typical inflammatory edema and arthritis-related symptoms on the contralateral side (i.e. left hindpaw). Both antinociceptive (evaluation of mechanical, thermal pain threshold and analysis of spinal Fos expression) and anti- inflammatory (evaluation of paw edema, serum interleukin-6 level and x-ray analysis) effect of BP extracts were examined. The ethylacetate fraction of BP (BPE) significantly suppressed the FCA-induced paw edema as well as the serum level of interleukin-6 and it alleviated the radiological changes. Moreover, both mechanical and thermal hyperalgesia were attenuated by the treatment of BPE. In addition, spinal Fos expression that was increased by FCA- injection was suppressed in BPE group. Therefore, this study showed that BPE produced significant both antinociceptive and anti-inflammatory effects on FCA- induced arthritis in rats, while hexane fraction of BP did not show these effects. In conclusion, it is suggested that the ethylacetate fraction of BP is recommended to alleviate the arthritis-related symptoms in human according to the results of this study.


Subject(s)
Analgesics/pharmacology , Animals , Anti-Inflammatory Agents/pharmacology , Arthritis, Experimental/drug therapy , Drugs, Chinese Herbal/pharmacology , Edema/veterinary , Hindlimb/diagnostic imaging , Hyperalgesia/veterinary , Interleukin-6/blood , Male , Pain Measurement/veterinary , Phytotherapy , Plant Extracts/pharmacology , Proto-Oncogene Proteins c-fos/metabolism , Rats , Rats, Sprague-Dawley , Spinal Cord/metabolism
20.
Medical Journal of Cairo University [The]. 1994; 62 (2): 419-432
in English | IMEMR | ID: emr-33435

ABSTRACT

This study was performed in conscious male albino normal rats to determine the changes in Ca+2 excretion and its concentration in the serum following oral administration of prostaglandin synthesis inhibition by tiaprofenic acid [10 mg/kg] and piroxicam [1 mg/kg] for 21 days. It was found that tiaprofenic acid reduced the mean +/- SE 24-hour urinary Ca+2 excretion significantly after 10 and 21 days. This reduction did not reverse after one month from stoppage of its administration. Serum Ca+2 was lowered significantly after 21 days from its administration and persisted after one month from withdrawal of the drug. Piroxicam reduced significantly Ca+2 excretion and serum concentration of Ca+2 after 10 days and more reduction appeared after 21 days from administration. This reduction was not reversed after one month from stoppage of its administration. On the other hand, in arthritic rats, tiaprofenic acid reduced significantly urinary Ca+2 excretion and serum Ca+2 after 10 days from its administration and more reduction was observed after 21 days. As regads arthritic rats receiving piroxicam, it was found that 24-hour urine volume, urinary excretion of Ca+2 concentration were decreased significantly after 10 days from its administration. This reduction became more apparent after 21 days. One month after withdrawal of tiaprofenic acid and piroxicam, some improvement in these parameters was observed but still a significant reduction was noted compared with the control group [arthritic rats]


Subject(s)
Piroxicam/pharmacology , Calcium/urine , Arthritis, Experimental/drug therapy , Calcium/blood , Regression Analysis/methods
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