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An. bras. dermatol ; 94(5): 574-577, Sept.-Oct. 2019. graf
Article in English | LILACS | ID: biblio-1054870


Abstract The authors report a case of 40-year-old male patient with a five-year history of chromoblastomycosis on his right leg. Diagnosis was performed by direct 40% KOH exam of skin scales, culture with micro- and macromorphologic analysis, and genotypic characterization (sequencing of a fragment of the ITS region and phylogenetic analysis) of the isolated fungus. Rhinocladiella aquaspersa was identified as the etiological agent. Initially, the treatment was with oral itraconazole 200 mg/day for one year. However, the presence of "sclerotic cells" with filaments ("Borelli spiders") resulted in a change of medical treatment: a higher dose of itraconazole (400 mg/day) and surgery, achieving clinical and mycological cure in one year. This is the first report of chromoblastomycosis caused by R. aquaspersa in Guatemala.

Humans , Male , Adult , Ascomycota/isolation & purification , Chromoblastomycosis/microbiology , Ascomycota/ultrastructure , Chromoblastomycosis/pathology , Chromoblastomycosis/drug therapy , Treatment Outcome , Itraconazole/therapeutic use , Guatemala , Antifungal Agents/therapeutic use
Mem. Inst. Oswaldo Cruz ; 100(2): 161-167, Apr. 2005. ilus, tab, graf
Article in English | LILACS | ID: lil-410854


Although lethargic crab disease (LCD) is causing massive mortalities in populations of the mangrove crab Ucides cordatus of Northeastern Brazil, the identity of its etiological agent was hitherto unknown. In this study we provide robust evidence suggesting that LCD is caused by an anamorph Ascomycota (Fungi). We examined specimens of U. cordatus collected from stocks affected by LCD. Histological and TEM methods detected the presence of hyphae, conidia, and condiophores in several host tissues. Moreover, the abundance of fungal stages is negatively associated with crab health. Finally, DNA was isolated from the fungus and a region of its 18S ribosomal gene was sequenced. Phylogenetic analyses not only confirm the diagnosis of the LCD fungus in crab tissues as an ascomycete, but also suggest a close relationship with members of the subphylum Pezizomycotina.

Animals , Female , Male , Ascomycota/isolation & purification , Brachyura/microbiology , Mycoses/veterinary , Ascomycota/genetics , Ascomycota/ultrastructure , Brazil , DNA, Fungal/genetics , Mycoses/microbiology , Phylogeny , /genetics