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1.
J. oral res. (Impresa) ; 10(4): 1-6, ago. 31, 2021. tab
Article in English | LILACS | ID: biblio-1393513

ABSTRACT

Objective: To determine the erosive effect of pieces of permanent teeth exposed to non-distilled alcoholic beverages. Material and Methods: This study takes a quantitative approach, with an explanatory scope, descriptive and correlational, with a pure experimental design. The sample consisted of 45 premolar permanent teeth, which were randomly allocated to five sample groups. Every sample group was submerged in dark beer Cuzqueña Negra, wheat beer Cuzqueña de Trigo, lager beer Cerveza Cristal, lager beer Pilsen Callao and physiological saline solution; every solution had a quantity of 110 mL. The experiment was performed for five minutes, with three repetitions every 12 hours over a period of 70 days. The sampling was performed every seven days, using an analytical scale and a data sheet. Results: The average weight loss of dental pieces put in dark beer Cuzqueña Negra (pH 4.0) was 239.4456 mg. In lager beer Pilsen Callao (pH 4.6), it was 146.7867 mg. In lager beer Cerveza Cristal (pH 3.7), it was 131.3567 mg. In wheat beer Cuzqueña de Trigo (pH 4.5), it was 121.7122 mg. Lastly, in physiological saline solution (pH 6.8), it was 14.3311 mg. When applied to the sample, the statistical test Student's t-test resulted in a value of p≈0.000 (p<0.05). Conclusion: Non-distilled alcoholic beverages caused erosive effects in the pieces of permanent teeth.


Objetivo: : Determinar el efecto erosivo en dientes permanentes expuestos a bebidas alcohólicas no destiladas. Material y Métodos: Este estudio tiene un enfoque cuantitativo, con alcance explicativo, descriptivo y correlacional, con un diseño puramente experimental. La muestra consistió en 45 dientes permanentes premolares, que fueron asignados aleatoriamente a cinco grupos de muestra. Cada grupo de muestra se sumergió en cerveza Cuzqueña Negra, cerveza Cuzqueña de Trigo, Cerveza Cristal, cerveza Pilsen Callao y solución salina fisiológica; cada solución tenía una cantidad de 110 mL. El experimento se realizó durante cinco minutos, con tres repeticiones cada 12 horas durante un período de 70 días. El muestreo se realizó cada siete días, utilizando una escala analítica y una hoja de datos. Resultados: La pérdida de peso promedio de las piezas dentales colocadas en cerveza negra Cuzqueña Negra (pH 4,0) fue de 239,4456 mg. En la cerveza Pilsen Callao (pH 4,6), fue de 146,7867 mg. En la cerveza Cerveza Cristal (pH 3,7) fue de 131,3567 mg. En cerveza Cuzqueña de Trigo (pH 4,5), fue de 121,7122 mg. Por último, en suero fisiológico (pH 6,8) fue de 14,3311 mg. Cuando se aplicó la prueba estadística t de Student a la muestra dio como resultado un valor de p?0,000 (p<0,05). Conclusion: Las bebidas alcohólicas no destiladas causaron efectos erosivos en dientes permanentes.


Subject(s)
Humans , Tooth Erosion , Dentition, Permanent , Alcoholic Beverages/adverse effects , Beer , Epidemiology, Descriptive , Saline Solution
2.
Chinese Journal of Biotechnology ; (12): 4373-4381, 2021.
Article in Chinese | WPRIM | ID: wpr-921513

ABSTRACT

Lager yeast is the most popular yeast strain used for beer production in China. The flocculation of yeast plays an important role in cell separation at the end of fermentation. Therefore, appropriately enhancing the flocculation capability of the lager yeast without affecting its fermentation performance would be desirable for beer industry. Our previous study showed that the defect of gene RIM21 might contribute to the enhanced flocculation capability of a lager yeast G03. To further investigate the role of the RIM21 gene in flocculation of strain G03, this study constructed a RIM21-deleted mutant strain G03-RIM21Δ through homologous recombination. Deletion of RIM21 improved the flocculation capability of strain G03 during wort fermentation at 11 °C without changing its fermentation performance significantly. The expression of FLO5, Lg-FLO1 and some other genes involved in cell wall integrity pathway were up-regulated in strain G03-RIM21Δ. In addition, the disruption of RIM21 enhanced resistance of yeast cells to cell wall inhibitors. These results provide a basis for elucidating the flocculation mechanism of lager yeast under low-temperature fermentation conditions.


Subject(s)
Beer , Fermentation , Flocculation , Receptors, Cell Surface , Saccharomyces/metabolism , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/metabolism
3.
Article in English | LILACS, BBO | ID: biblio-1101305

ABSTRACT

Abstract Objective: To evaluate the erosive potential of different alcoholic beverages according to pH, titratable acidity and buffering capacity. Material and Methods: Thirteen industrialized alcoholic beverages of different brands were selected and divided into five groups according to their type and composition. The pH measurement and titratable acidity for pH 5.5 and 7.0 were performed in triplicate in 50 mL of each beverage. The buffering capacity was calculated based on pH and titratable acidity for pH 7.0. ANOVA, Tukey, and Pearson correlation, with p<0.05, were used for data analysis. Results: Data showed normal distribution by Shapiro-Wilk test. The pH of alcoholic beverages ranged from 2.49 (Miks Ice Tea - Green Fruits) to 7.64 (Smirnoff). The highest values of acid titration (4.68) and buffer capacity (19.97) were observed in Smirnoff Ice. The following correlations (p<0.01) were noted between: pH and titratable acidity; buffering capacity and pH; buffering capacity and titratable acidity. Conclusion: Some beers and alcopops presented erosive potential due to their lower pH associated with high acid titration values. The whisky and sugarcane liquor examined were not potentially erosive.


Subject(s)
Tooth Erosion/etiology , Carbonated Beverages/adverse effects , Alcoholic Beverages/adverse effects , Acidity/adverse effects , Hydrogen-Ion Concentration , Beer , Brazil/epidemiology , Normal Distribution , Analysis of Variance , Statistics, Nonparametric , Saccharum
4.
Braz. arch. biol. technol ; 63: e20190229, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132245

ABSTRACT

Abstract To develop a biorefinery concept applied in the brewery industry, Chlorella pyrenoidosa and a consortium of associated bacteria were cultivated mixotrophically in a continuous photobioreactor using brewery low-value subproducts as an integrative process. Beer production residues were biochemically characterized to assess the most promising options to be used as a nutrient source for microalgal cultivation. Due to its physical and chemical properties, pre-treated weak wort was used to prepare an organic complex culture medium for microalgal biotransformation. Filtration and nitrogen supplementation were necessary to improve nutrient removal and biomass productivity. Maximal removal of nitrate and phosphate obtained were 90% and 100% respectively. Depending on operation conditions, total carbohydrates depuration ranged from 50 - 80%. The initial concentration of total carbohydrates of the weak wort must be adjusted to 2 - 4g/L to maintain a stable equilibrium between microalgal and bacterial growth. The biochemical composition of produced biomass varied depending on the cultivation conditions as well as on its final use. Upon continuous mixotrophic conditions evaluated in this study, C. pyrenoidosa was composed mainly of carbohydrates and protein.


Subject(s)
Animals , Beer , Biochemical Phenomena , Biotransformation , Chlorella/growth & development , Microalgae/growth & development , Carbohydrates , Chlorella/chemistry , Biomass , Photobioreactors/microbiology
6.
Licere (Online) ; 22(3): 34-63, set.2019.
Article in Portuguese | LILACS | ID: biblio-1022750

ABSTRACT

A produção de cerveja caseira é um fenômeno social que pode ser empiricamente observado e associado ao campo de Estudos do Lazer. Este texto apresenta e avança em relação aos resultados parciais da tese de doutorado intitulada "Da panela ao copo: a produção de cerveja como prática de lazer", e abordou aspectos sobre um grupo de cervejeiros caseiros de Belo Horizonte ­ MG e as interfaces desta prática com o lazer. Como estudo qualitativo com características etnográficas, foram realizadas entrevistas semiestruturadas, acompanhamento de brassagens e produção de cerveja em casa por um dos autores, com registro em diário de campo. Observou-se que os cervejeiros compõem um grupo social, majoritariamente masculino, culturalmente próximo, inserido no mercado de trabalho e que possuem em comum as variáveis tempo, espaço e dinheiro para vivenciar seu lazer.


Craft brewering is a social phenomenon that can be empirically observed and associated with the Leisure Studies. This paper presents and goes through to partial results of a doctoral thesis named "From the pot until the glass: brewering as leisure practice" and deal with aspects of a group of craft brewers in Belo Horizonte, city in Minas Gerais state, Brazil, such as the relationship of that practice with leisure. As a qualitative study with ethnographic characteristic, interviews has been made, participation in brewering sessions and craft brewering at home made by one of the authors, registered in a field journal. The brewers can be seen as a social group, mainly males, with a cultural approach, inserted in labor world and with common characteristics free time, space and financial resources for leisure living.


Subject(s)
Humans , Male , Adult , Beer , Job Market , Sociological Factors , Popular Culture , Acculturation , Leisure Activities
7.
Chinese Journal of Biotechnology ; (12): 522-534, 2019.
Article in Chinese | WPRIM | ID: wpr-771355

ABSTRACT

As a new beer fermentation technology, high temperature and high gravity fermentation has brought many benefits to brewery industry, but there are also a series of problems such as the decrease of yeast flocculation ability at the end of fermentation and the high concentration of higher alcohols. To increase yeast flocculation ability and reduce the production of higher alcohols in high temperature and high gravity fermentation of beer, BAT2 was replaced by the FLO5 expression cassette to obtain the mutant strain S6-BF2. Real-time quantitative PCR showed that the relative transcriptional level of FLO5 in S6-BF2 improved 17.8 times compared with that in S6. The flocculation ability of mutant S6-BF2 heightened by 63% compared to that of the original strain S6, and the concentration of higher alcohols decreased from 175.58 mg/L to 159.58 mg/L in high temperature and high gravity fermentation of beer. Moreover, the activity of mitochondrial branched-chain amino acid transferase was repressed, resulting in the production of higher alcohols of 142.13 mg/L, reduced by 18.4% compared to that of the original strain S6, meanwhile, the flocculation ability of mutant S6-BF2B1 kept unchanged compared to the mutant S6-BF2. The determination result of flavor compounds showed that the higher alcohols/ester ratio in beer was reasonable. This research has suggested an effective strategy for enhancing yeast flocculation ability and decreasing production of higher alcohols in high-temperature and high-gravity brewing.


Subject(s)
Beer , Fermentation , Hypergravity , Saccharomyces cerevisiae , Saccharomyces cerevisiae Proteins , Temperature , Transaminases
8.
Article in English | WPRIM | ID: wpr-741700

ABSTRACT

BACKGROUND/OBJECTIVES: The NAD+ precursor nicotinamide riboside (NR) is a type of vitamin B3 found in cow's milk and yeast-containing food products such as beer. Recent studies suggested that NR prevents hearing loss, high-fat diet-induced obesity, Alzheimer's disease, and mitochondrial myopathy. The objective of this study was to investigate the effects of NR on inflammation and mitochondrial biogenesis in AML12 mouse hepatocytes. MATERIALS/METHODS: A subset of hepatocytes was treated with palmitic acid (PA; 250 µM) for 48 h to induce hepatocyte steatosis. The hepatocytes were treated with NR (10 µM and 10 mM) for 24 h with and without PA. The cell viability and the levels of sirtuins, inflammatory markers, and mitochondrial markers were analyzed. RESULTS: Cytotoxicity of NR was examined by PrestoBlue assay. Exposure to NR had no effect on cell viability or morphology. Gene expression of sirtuin 1 (Sirt1) and Sirt3 was significantly upregulated by NR in PA-treated hepatocytes. However, Sirt1 activities were increased in hepatocytes treated with low-dose NR. Hepatic pro-inflammatory markers including tumor necrosis factor-alpha and interleukin-6 were decreased in NR-treated cells. NR upregulated anti-inflammatory molecule adiponectin, and, tended to down-regulate hepatokine fetuin-A in PA-treated hepatocytes, suggesting its inverse regulation on these cytokines. NR increased levels of mitochondrial markers including peroxisome proliferator-activated receptor γ coactivator-1α, carnitine palmitoyltransferase 1, uncoupling protein 2, transcription factor A, mitochondrial and mitochondrial DNA in PA-treated hepatocytes. CONCLUSIONS: These data demonstrated that NR attenuated hepatic inflammation and increased levels of mitochondrial markers in hepatocytes.


Subject(s)
Adiponectin , alpha-2-HS-Glycoprotein , Alzheimer Disease , Animals , Beer , Carnitine O-Palmitoyltransferase , Cell Survival , Cytokines , DNA, Mitochondrial , Fatty Liver , Gene Expression , Hearing Loss , Hepatocytes , Inflammation , Interleukin-6 , Mice , Milk , Mitochondria , Mitochondrial Myopathies , Niacin , Niacinamide , Obesity , Organelle Biogenesis , Palmitic Acid , Peroxisomes , Sirtuin 1 , Sirtuins , Transcription Factors , Tumor Necrosis Factor-alpha
9.
Article in Korean | WPRIM | ID: wpr-766002

ABSTRACT

PURPOSE: The consumption of processed foods has recently been increasing due to changes in the living environment. The purpose of this study was to identify the contribution of processed food to the nutrient intake of adult Koreans. METHODS: A total of 15,760 adult people in the 6(th) National Health and Nutrition Examination Survey (2013 ~ 2015) were included in this study. According to the Korea Food and Drug Administration's classification criteria for processed foods, the 24 hour dietary recall data of the subjects were classified as processed food or natural food. The processed food intake, nutrient intake and major processed food sources by food groups were analyzed. RESULTS: Men consumed more processed foods than did the women. Consumption of processed foods decreased with age, but it increased with the education level and the income level. The total daily processed food intake accounted for 68.1% of the total food intake. The food groups with high processed food intake were beverage, vegetables, cereals and grain products, fruits, and milk and dairy products in this order. The top food source of each food groups were beer, kimchi, bread, processed apple products, and milk. After adjusting for age, gender, and energy intake, all the nutrient intakes and percentage of dietary reference intakes for Koreans, except carbohydrates, were significantly higher in processed foods than in natural foods. The sodium intake from the processed food was 96.3% of total daily sodium intake. The intakes of nutrients from processed foods, excluding vitamins C, dietary fiber, iron, and vitamin A, were higher in men than in women. The intake of sodium from processed foods was highest for people of 30 ~ 49 years of age, and the intake of sodium from processed foods decreased for people over 50. CONCLUSION: Korean adults consumed more processed food than the natural food, consuming more calories and most of the nutrients from the processed food overall total daily intakes. The intake of processed foods is expected to further increase in the future, and nutritional education and research on the ingestion and selection of healthy processed foods are necessary.


Subject(s)
Adult , Beer , Beverages , Bread , Carbohydrates , Classification , Dairy Products , Dietary Fiber , Eating , Edible Grain , Education , Energy Intake , Female , Fruit , Humans , Iron , Korea , Male , Milk , Nutrition Surveys , Recommended Dietary Allowances , Sodium , Vegetables , Vitamin A , Vitamins
10.
Chinese Journal of Biotechnology ; (12): 1059-1070, 2019.
Article in Chinese | WPRIM | ID: wpr-771822

ABSTRACT

The autolysis of brewer's yeast seriously affects the quality of beer and the quality of yeast is considered as one of the key factors in beer brewing. Previous studies on brewer's yeast autolysis showed that RLM1 gene, an important transcription factor in cell integrity pathway, is closely related to the autolysis of yeast. In this study, RLM1 was knocked out and overexpressed in a haploid brewer's yeast. RLM1 disruption resulted in poor anti-autolysis performance of yeast, whereas overexpression of RLM1 contributed to the anti-autolytic ability of yeast. In addition, RLM1 gene knockout affected the osmotic stress resistance, cell wall damage resistance, nitrogen starvation resistance and temperature tolerance of yeast strain. The transcriptional level of GAS1 involved in cell wall assembly and DNA damage response was regulated along with the expression of RLM1, whereas other genes in CWI pathway did not show apparent regularity. RLM1 might mainly affect the expression of GAS1 so as to improve the stress resistance of lager yeast in harsh environment. The result from this study help further understand the mechanism of yeast autolysis and lay a foundation for breeding brewer's yeast strain with better anti-autolytic ability.


Subject(s)
Autolysis , Beer , Cell Wall , Humans , MADS Domain Proteins , Saccharomyces cerevisiae , Saccharomyces cerevisiae Proteins
11.
Braz. j. microbiol ; 49(4): 808-815, Oct.-Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-974288

ABSTRACT

ABSTRACT Chicha, a type of beer made mainly with maize or cassava, is a traditional fermented beverage of the Andean region. There have only been a few studies on yeasts associated with chicha fermentation, and the species diversity occurring during the production of this beverage is not known. The objective of this study was to determine the biodiversity of yeasts in chicha, and to characterize the Saccharomyces cerevisiae populations associated with the production of chicha de jora, seven-grain chicha, chicha de yuca, and chicha de morocho in Ecuador. The molecular diversity of S. cerevisiae populations was determined by restriction polymorphism mitochondrial profiles. The beverages were characterized based on their physicochemical parameters. Twenty-six species were identified, and the most prevalent species were S. cerevisiae and Torulaspora delbrueckii. Other yeast species were isolated at low frequencies. Among 121 isolates of S. cerevisiae, 68 different mtDNA molecular profiles were identified. These results showed that chichas are fermented by a high number of different strains of S. cerevisiae. Some other species provided a minor contribution to the fermentation process. The chicha presented generally similar physicochemical parameters to those observed for other traditional fermented beverages, and can be considered as an acid fermented beverage.


Subject(s)
Saccharomyces cerevisiae/isolation & purification , Beer/microbiology , Yeasts/isolation & purification , Yeasts/metabolism , Saccharomyces cerevisiae/classification , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Beer/analysis , Yeasts/classification , Yeasts/genetics , Manihot/metabolism , Manihot/microbiology , Zea mays/metabolism , Zea mays/microbiology , Biodiversity , Ecuador , Fermentation
12.
Hig. Alimentar ; 32(280/281): 23-26, 30/06/2018. ilus
Article in Portuguese | LILACS | ID: biblio-910117

ABSTRACT

Diante do grande consumo de bebidas alcoólicas no Brasil e no mundo, muitas empresas têm investido na confecção de embalagens cada vez mais promissoras. Com o objetivo de conhecer o perfil de consumo de cervejas e vinhos relacionado às suas respectivas embalagens, foi desenvolvida uma pesquisa na cidade de Salvador-BA, sendo entrevistadas 221 pessoas cujo consumo das bebidas estava intrinsecamente ligado ao ambiente social. A prevalência de consumo é das cervejas com cerca de 26,7%, sendo que os principais locais de dispêndio com a mesma são festas e bares. Vale salientar que a embalagem escolhida por 93,3% dos participantes é a de vidro. Observa-se então um crescimento favorável das indústrias cervejeiras no Brasil com a comercialização deste produto popular em detrimento do vinho, possuinte de um estilo mais elitizado e restrito de consumo.


Due to the large consumption of alcoholic beverages in Brazil and in the world, many companies have made investments to increase the design of its packaging. In order to discover the relation in between the consumption of beers, wine and its packages, a research has been developed in Salvador (BA). Therefore, 221 people were interviewed, concluding that the beverage choice was intrinsically related to the social environment. Furthermore, this study highlights the predominance of beers in front of wines consumption. This scientific enquire points the development of beers industries in Brazil, but also spotlight the decrease of wine consumption due to its elitist stigma.


Subject(s)
Wine , Alcohol Drinking , Brewery , Product Packaging , Alcoholic Beverages , Beer , Brazil , Food Packaging , Qualitative Research
13.
Article in Korean | WPRIM | ID: wpr-759665

ABSTRACT

This study was conducted to analyze the effects of beverages on tooth corrosion by selecting drinks that are enjoyed by modern people. Drinks were selected for carbonated beverages (Coca Cola), energy drinks (Red Bull), orange juice, beer (Hite) and water (Evian). Bovine tooth was immersed for 1 hour, 24 hours and 48 hours. The root mean square (RMS) values were obtained by scanning the bovine tooth at pre-immersion and immersion time after impression making. Two-way ANOVA, one-way ANOVA, and Tukey-test were used to compare differences between groups (α=0.05). The RMS value tended to increase with longer immersion times in all beverages, and there was a significant difference (p < 0.05) between before immersion-1hr and before immersion-48 hr. The mean of RMS according to the type of beverage was significantly different and there was a significant difference according to the change of immersion time (p < 0.05). In order to prevent tooth corrosion, it is necessary to change the method of ingestion or to reduce the time of ingestion.


Subject(s)
Beer , Beverages , Carbonated Beverages , Citrus sinensis , Corrosion , Eating , Energy Drinks , Immersion , Methods , Tooth , Water
14.
Braz. j. microbiol ; 48(4): 740-746, Oct.-Dec. 2017. tab, graf
Article in English | LILACS | ID: biblio-889173

ABSTRACT

ABSTRACT The spoilage of beer by bacteria is of great concern to the brewer as this can lead to turbidity and abnormal flavors. The polymerase chain reaction (PCR) method for detection of beer-spoilage bacteria is highly specific and provides results much faster than traditional microbiology techniques. However, one of the drawbacks is the inability to differentiate between live and dead cells. In this paper, the combination of propidium monoazide (PMA) pretreatment and conventional PCR had been described. The established PMA-PCR identified beer spoilage Lactobacillus brevis based not on their identity, but on the presence of horA gene which we show to be highly correlated with the ability of beer spoilage LAB to grow in beer. The results suggested that the use of 30 µg/mL or less of PMA did not inhibit the PCR amplification of DNA derived from viable L. brevis cells. The minimum amount of PMA to completely inhibit the PCR amplification of DNA derived from dead L. brevis cells was 2.0 µg/mL. The detection limit of PMA-PCR assay described here was found to be 10 colony forming units (CFU)/reaction for the horA gene. Moreover, the horA-specific PMA-PCR assays were subjected to 18 reference isolates, representing 100% specificity with no false positive amplification observed. Overall the use of horA-specific PMA-PCR allows for a substantial reduction in the time required for detection of potential beer spoilage L. brevis and efficiently differentiates between viable and nonviable cells.


Subject(s)
Staining and Labeling/methods , Beer/microbiology , Lactobacillus brevis/isolation & purification , Lactobacillus brevis/growth & development , Real-Time Polymerase Chain Reaction/methods , Propidium/analogs & derivatives , Propidium/chemistry , Azides/chemistry , Lactobacillus brevis/genetics , Lactobacillus brevis/chemistry , Real-Time Polymerase Chain Reaction/instrumentation , Food Microbiology
15.
Electron. j. biotechnol ; 30: 1-5, nov. 2017. ilus, tab, graf
Article in English | LILACS | ID: biblio-1021034

ABSTRACT

Background: The enzymes utilized in the process of beer production are generally sensitive to higher temperatures. About 60% of them are deactivated in drying the malt that limits the utilization of starting material in the fermentation process. Gene transfer from thermophilic bacteria is a promising tool for producing barley grains harboring thermotolerant enzymes. Results: Gene for α-amylase from hydrothermal Thermococcus, optimally active at 75­85°C and pH between 5.0 and 5.5, was adapted in silico to barley codon usage. The corresponding sequence was put under control of the endosperm-specific promoter 1Dx5 and after synthesis and cloning transferred into barley by biolistics. In addition to model cultivar Golden Promise we transformed three Slovak barley cultivars Pribina, Levan and Nitran, and transgenic plants were obtained. Expression of the ~50 kDa active recombinant enzyme in grains of cvs. Pribina and Nitran resulted in retaining up to 9.39% of enzyme activity upon heating to 75°C, which is more than 4 times higher compared to non-transgenic controls. In the model cv. Golden Promise the grain α-amylase activity upon heating was above 9% either, however, the effects of the introduced enzyme were less pronounced (only 1.22 fold difference compared with non-transgenic barley). Conclusions: Expression of the synthetic gene in barley enhanced the residual α-amylase activity in grains at high temperatures.


Subject(s)
Seeds/enzymology , Hordeum/enzymology , Thermococcus/metabolism , alpha-Amylases/metabolism , Seeds/genetics , Seeds/microbiology , Transformation, Genetic , Hordeum/genetics , Hordeum/microbiology , Beer , Enzyme Stability , Plants, Genetically Modified/enzymology , Cloning, Molecular , Gene Transfer Techniques , alpha-Amylases/genetics , Fermentation , Thermotolerance , Hot Temperature , Hydrogen-Ion Concentration
16.
Braz. J. Pharm. Sci. (Online) ; 53(4): e17141, 2017. tab
Article in English | LILACS | ID: biblio-889423

ABSTRACT

ABSTRACT Alcohol is the most commonly consumed substance in the world. The objective of this study was to evaluate the influence of alcoholic beverages on male reproduction and possible alterations in their offspring. The mice were divided into 4 groups: beer, wine, cachaça (a type of sugarcane rum), with ethanol concentrations of 1.9 g/kg, and control group treated with PBS. The treatment period was 35 days. The animals which received cachaça, demonstrated significant weight loss in the testes and epididymis. The alcoholic beverages promoted significant testosterone level and fertilization index diminution, and morphological alterations in the spermatozoa. The beer group presented decreased implantation sites and a high frequency of dominant lethal. The number of reabsorptions in the wine group was increased. The fermented beverages presented higher potential to induce visceral malformations, while the cachaça caused fetal skeletal malformations. The cachaça treated group presented a negative impact on semen quality and fertilization potential. The treatment with different alcoholic beverages, during spermatogenesis, demonstrated contrasting degrees of induction of toxic effects, interfering in a general aspect in male reproductive performance, fetal viability during intrauterine life, and birth defects. From the data, it is possible to infer that the distillated beverage caused more harmful effects to reproduction in this study.


Subject(s)
Animals , Male , Female , Mice , Reproduction/drug effects , Wine/adverse effects , Alcoholic Beverages/analysis , Fertilization , Beer/adverse effects , Erectile Dysfunction/physiopathology , Infertility, Male/prevention & control
17.
Acta Medica Philippina ; : 116-120, 2017.
Article in English | WPRIM | ID: wpr-633360

ABSTRACT

OBJECTIVE: Alcohol consumption of university students in Metro Manila was determined and compared to other countries in the region and in the West.METHODS: A survey of students from ten randomly selected public and private universities in Metro Manila was done.RESULTS: A total of 2,930 students participated, 46.1% from public schools and 58.9% were female. Of the total students, 81.6% had drank an alcoholic drink in their lifetime; and 15.6% drank beer, 22.5% drank wine and 11.4% drank distilled spirits before they were 13 years old. For the past 30 days, 30.2% drank beer, 30.8% distilled spirit and 18.7% wine; 16.9%, 7.4% and 19.7% binged on beer, wine and distilled spirits, respectively; and 29.1% got drunk. Five percent of the students had drank inside their school. Of those who drove any motorized vehicle, 28.9% drove when they had drank and of those who use prohibited drugs, 66.7% mixed the two. Thirty one percent said they tried to quit drinking in the past 12 months and 7.5% admitted they have a drinking problem.CONCLUSION: Alcohol consumption of Metro Manila students approximates those of other countries. For policy and monitoring purposes, it is recommended that this survey be done regularly with a nationwide scope and compare this with other countries for best practices.


Subject(s)
Humans , Male , Female , Young Adult , Adolescent , Universities , Beer , Wine , Alcoholic Beverages , Alcohol Drinking , Alcoholic Intoxication , Alcoholism , Students , Surveys and Questionnaires
18.
Rev. costarric. salud pública ; 25(2): 44-53, jul.-dic. 2016. tab, ilus
Article in Spanish | LILACS | ID: biblio-844771

ABSTRACT

ResumenObjetivo:Analizar la relación del consumo de cerveza sin alcohol (fuente de antioxidantes) con los valores del perfil de lípidos de un grupo de adultos del cantón San Mateo de Alajuela durante 35 días.Métodos:La condición nutricional y el perfil lipídico de los adultos, que participaron en el presente estudio se obtuvo mediante una anamnesis nutricional; Esto incluyó, en primer lugar, una evaluación antropométrica. En segundo lugar, el análisis sanguíneo de los perfiles lipídicos tanto antes como después del consumo de una cierta cantidad de cerveza no alcohólica (0,5 l por día) durante un período de 35 días; Finalmente, una dieta basada en los hábitos de alimentación y su valor nutricional.Resultados:Los sujetos estudiados presentaron varios factores de riesgo asociados con el desarrollo de enfermedades cardiovasculares, sobresalieron: el sobrepeso, la obesidad, el consumo excesivo de grasas y carbohidratos simples, deficiencia de frutas, vegetales, alimentos integrales, y el sedentarismo. El consumo moderado de cerveza demostró mejorar los valores de Colesterol HDL en la mayoría de los participantes; por el contrario, el Colesterol Total, Colesterol LDL y Triglicéridos presentaron variaciones.Conclusión:La investigación evidenció mejorías en algunos indicadores del perfil de lípidos; sin embargo, se necesita investigar y ahondar más en el efecto de un consumo moderado de cerveza sin alcohol en la salud de grupos más disciplinados y controlados.


AbstractObjetive:To analyze the relation between the consumption of nonalcoholic beer and nourishment as well as the lipid profile values of a group of adults from the canton of San Mateo, Alajuela in a 35-day period.Methods:The nutritional condition and the lipid profile of the adults, that took part in the current study gotten by means of a nutritional anamnesis; this included, first, an anthropometric evaluation. Second, the blood analysis of lipid profiles both before and after the consumption of a certain amount of nonalcoholic beer (0.5 l per day) during a 35 day- period; finally, a diet based on feeding habits and their nutritional value.Results:The studied people presented several risk factors associated with the development of cardiovascular illnesses; among others, overweight, obesity, nourishment eating habits that promote an excessive consumption of fat and simple carbohydrates, a deficient consumption of fruits, vegetables, whole-grain sources, and sedentary lifestyle. A moderate consumption of nonalcoholic beer showed a development in HDL cholesterol in most patients; as opposed, the global cholesterol, the LDL cholesterol and triglycerides showed given variations related to the cardiovascular risk factors of patients under study.Conclusion:This research showed evidenced of gan improvement in the lipid profile indicators; however, a further investigation is required for testing the effects of moderated consumption of nonalcoholic beer in a more disciplinary and medicine-controlled population of adults. Besides, a relationship between good eating habits and physical activities to normal lipid profiles was demonstrated


Subject(s)
Humans , Male , Female , Adult , Beer , Diet, Food, and Nutrition , Costa Rica
19.
Hig. Alimentar ; 30(256/257): 142-147, maio/junho 2016. ilus
Article in Portuguese | LILACS | ID: biblio-1703

ABSTRACT

A cerveja é uma bebida alcoólica comercializada frequentemente em latas de alumínio devido à praticidade no consumo. Este tipo de embalagem entra em contato direto com a boca do consumidor e necessita de um cuidado redobrado com relação aos aspectos de higienização para não ser um veículo de micro-organismos patogênicos. O trabalho objetivou avaliar a presença de micro-organismos aeróbios mesófilos e enterobactérias nas superfícies das latas de cerveja comercializadas no município de Itabuna, Bahia. Foram coletadas 48 latas de cerveja (24 com vendedores ambulantes e 24 em supermercados) no mês de setembro de 2012 e realizou-se a Técnica do Esfregaço de Superfície com plaqueamento do inóculo em profundidade nos meios de cultura PCA e VRBD. A presença dos micro-organismos aeróbios mesófilos foi determinada com contagens acima de 300 UFC/cm², em 70,8% e 12,5% das amostras coletadas com ambulantes e em supermercados, respectivamente. Já as enterobactérias tiveram presença acima de 300 UFC/cm² em 37,5% das amostras dos ambulantes e contagens abaixo de 50 UFC/cm² em 100% das amostras coletadas em supermercados. As altas contagens de micro-organismos aeróbios mesófilos e enterobactérias na superfície das latas coletadas com ambulantes indicaram a necessidade de boas práticas higiênicas na comercialização da bebida.


Beer is an alcoholic beverage often marketed in aluminum cans because of the convenience in use. This type of packaging is in direct contact with the consumer's mouth and needs a special care regarding the hygiene aspects not to be a vehicle of pathogenic microorganisms. The study aimed to evaluate the presence of mesophilic aerobic microorganisms and enterobacteria on the surfaces of cans of beer sold in the city of Itabuna, Bahia. They collected 48 cans of beer (24 with street vendors and 24 supermarkets) in September 2012 and held the surface smear technique with the plating of inoculum in depth in the PCA and VRBD culture media. The presence of mesophilic aerobic microorganisms was determined above 300 CFU / cm² counts in 70.8% and 12.5% of the samples collected from street and supermarkets, respectively. Enterobacteria already had presence above 300 CFU / cm² in 37.5% of samples of street and scores below 50 CFU / cm² at 100% of the samples collected in supermarkets. The high counts of mesophilic aerobic microorganisms and enterobacteria on the surface of cans collected with street indicated the need for good hygienic practices in the marketing of the drink.


Subject(s)
Beer/microbiology , /analysis , Food Packaging/standards , Alcoholic Beverages , Microbiology , Brazil , Enterobacteriaceae/isolation & purification , Street Food , Products Commerce
20.
Hig. Alimentar ; 30(254/255): 128-133, 30/04/2016.
Article in Portuguese | LILACS | ID: biblio-836693

ABSTRACT

A introdução do bagaço cervejeiro na alimentação humana, como alternativa de reutilizá-lo, é também uma opção de enriquecimento da alimentação, visto que este carrega consigo uma rica quantidade de nutrientes. O objetivo deste trabalho foi avaliar a aceitação e a intenção de compra de pão e biscoito elaborados com farinha do bagaço cervejeiro. Para isso, o bagaço foi desidratado e moído para obtenção da farinha e adicionado em 20% da quantidade de farinha de trigo para elaboração dos produtos. Após realizou-se análise sensorial com 56 provadores não treinados com auxílio de escala hedônica de nove pontos para análise de aparência, odor, textura e sabor. Com os resultados calcularam-se as médias e os índices de aceitabilidade. Os produtos elaborados obtiveram ótima aceitação pelos provadores, onde o sabor apresentou maior índice de aceitação em ambos os produtos. Em relação à intenção de compra, o pão despertou maior interesse, já que a maioria compraria com certeza. Percebeu-se que os produtos podem obter sucesso de compra se produzidos e lançados ao mercado, pois os resultados foram sensorialmente satisfatórios, segundo as médias e o índice de aceitação.


Subject(s)
Humans , Beer/analysis , Bread/analysis , Functional Food , Flour Benefactor , Brewery , Consumer Behavior
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