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1.
Braz. j. microbiol ; 49(1): 104-111, Jan.-Mar. 2018. tab, graf
Article in English | LILACS | ID: biblio-889207

ABSTRACT

ABSTRACT Despite the increasing reports on the incidence of fresh vegetables and fruits as a possible vehicle for human pathogens, there is currently limited knowledge on the growth potential of Escherichia coli O157:H7 on different plant substrates. This study analyzed the selective adhesion and growth of E. coli O157:H7 on chili habanero (Capsicum chinense L.), cucumber (Cucumis sativus), radish (Raphanus sativus), tomato (Lycopersicon esculentum), beet (Beta vulgaris subsp. vulgaris), and onion (Allium cepa L.) under laboratory conditions. The Gompertz parameters were used to determine the growth kinetics. Scanning electron microscopy was used to visualize the adhesion of E. coli O157:H7 on the epicarp of the samples. Predictive models were constructed to compare the growth of E. coli O157:H7 on the samples with different intrinsic factors and to demonstrate the low selectivity of the pathogen. No significant difference was observed in the lag-phase duration (LPD), generation time (GT), and exponential growth rate (EGR) of the pathogen adhered to the samples. The interaction between the microorganism and the substrate was less supportive to the growth of E. coli O157:H7 for onion, whereas for tomato and cucumber, the time for the microorganism to attain the maximum growth rate (M) was significantly longer than that recorded for other samples.


Subject(s)
Vegetables/microbiology , Escherichia coli O157/growth & development , Fruit/microbiology , Capsicum/microbiology , Kinetics , Food Contamination/analysis , Lycopersicon esculentum/microbiology , Cucumis sativus/microbiology , Escherichia coli O157/isolation & purification , Escherichia coli O157/genetics , Escherichia coli O157/chemistry , Onions/microbiology , Beta vulgaris/microbiology
2.
Braz. j. med. biol. res ; 51(11): e7722, 2018. tab, graf
Article in English | LILACS | ID: biblio-974246

ABSTRACT

This study was designed to provide laboratory evidence supporting the hematopoietic effect of Beta vulgaris (beet) leaf aqueous extract in phenylhydrazine-induced anemia model in albino rats. Extraction of the leaves/stalks was done by maceration in 30% hydro-ethanol for 48 h. An intraperitoneal injection of 20 mg/kg phenylhydrazine was applied for two consecutive days to develop hemolytic anemia on the 4th day after the 1st injection in 24 of 30 male albino rats. The animals were divided into 5 groups and received the following treatments: standard (ferrous ascorbate + folic acid; 13.5 + 0.135 mg/kg), B. vulgaris extract (100 and 200 mg/kg), or left untreated (normal and diseased controls). Blood samples were taken at 0, 4, 8, and 12 days of the experiment for hematological and clinico-chemical analysis. Beet leaf extract significantly restored the levels of red blood cells, white blood cells, hemoglobin, and hematocrit in dose- and time-dependent manners. Blood indices have been significantly corrected. Erythropoietin level was maintained at higher levels. Erythrocytic membrane oxidation biomarker (malondialdehyde) level was significantly reduced compared to the anemic untreated group. The extract exhibited potent, concentration (4-512 μg/mL)-dependent antioxidant activity indicated by the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, with IC50 value of 37.91 μg/mL. Beet leaf extract resulted in detection of flavonoid and phenolic compounds that may underlie its hematinic properties. These findings may indicate B. vulgaris as a good natural source for pharmaceutical preparations with hematopoietic effects and treatment of anemia and/or associated conditions.


Subject(s)
Animals , Male , Rats , Plant Extracts/pharmacology , Plant Leaves/chemistry , Beta vulgaris/chemistry , Hematinics/pharmacology , Anemia/drug therapy , Phenylhydrazines , Time Factors , Disease Models, Animal , Anemia/chemically induced , Anemia/blood
3.
Rev. chil. nutr ; 45(2): 178-182, 2018.
Article in Spanish | LILACS | ID: biblio-959477

ABSTRACT

RESUMEN Los cultivos agrícolas pertenecientes al grupo Beta vulgaris L. actualmente se utilizan en la agroindustria para producción de azúcar, agentes colorantes, consumo fresco y otros sub productos. La betarraga (beta vulgaris L) es una hortaliza con gran cantidad de carbohidratos, potasio y sales de nitrato. El foco de esta revisión se centró en demostrar la influencia de los compuestos bioactivos de betarraga (Beta vulgaris L) sobre el efecto cardio-protector. Se analizaron estudios genéricos y específicos de betarraga. Ellos mostraron que el contenido total de polifenoles en betarraga varia de 218.00 mg.kg-1 a 887.75 mg.kg-1; el contenido total de antocianinas varía de 14.48 ± 0.40 mg.kg-1 a 84.50 ± 4.71 mg.kg-1; los valores de actividad antioxidante variaron en el intervalo de 8.37 ± 0.29% a 21.83 ± 0.35% y el contenido de sales de nitrato se estima en 1800 mg NO3-/kg de masa fresca. Al consumo de betarraga se le atribuyen efectos sobre la salud, entre ellos cardio-protectores y quimioterapéuticos relacionados con los polifenoles, antocianinas, sales de nitrato.


ABSTRACT The agricultural crops that belong to the group Beta vulgaris L are currently used in agroindustry to produce sugar, coloring agents, other sub products and for fresh consumption. Beetroot (beta vulgaris L) is a vegetable with a large amount of carbohydrates, potassium and nitrate salts. The focus of this review was to demonstrate the influence of the bioactive compounds of beetroot (Beta vulgaris L) on the cardioprotective effect. We analyzed generic and specific studies of beetroot. They showed that the total content of polyphenols in beetroot varied from 218.00 mg.kg-1 to 887.75 mg.kg-1; the total content of anthocyanins varied from 14.48 ± 0.40 mg.kg-1 to 84.50 ± 4.71 mg.kg-1; the value of antioxidant activity varied in range from 8.37 ± 0.29% to 21.83 ± 0.35%; and the content of nitrate salts was estimated at 1800 mg NO3-/kg of fresh mass. thus, the intake of beetroot is attributed to health effects including caridioprotectors and chemotherapeutic related to polyphenols, anthocyanins, nitrate salts.


Subject(s)
Humans , Beta vulgaris , Heart , Antioxidants , Polyphenols , Anthocyanins , Nitrates
4.
Arch. pediatr. Urug ; 88(6): 335-340, oct. 2017. tab
Article in Spanish | LILACS | ID: biblio-887803

ABSTRACT

La metahemoglobinemia es un síndrome clínico dado por la presencia de una forma aberrante de hemoglobina, ocasionada por diversos agentes oxidantes. Se describe un caso clínico de metahemoglobinemia severa asociada a la ingesta de puré de acelgas con alto contenido en nitratos y nitritos. Paciente de un año, con antecedentes de comunicación interauricular (CIA), que presentó un cambio en coloración de la piel 7 h antes, en forma progresiva, acompañado de vómitos. Ingresó al Departamento de Emergencia con cianosis generalizada que no mejoró con oxigenoterapia, taquicardia y tendencia a hipotensión arterial. En cuidados intensivos se realizó ecocardiograma que evidenció CIA sin repercusión hemodinámica. Metahemoglobinemia 37%. Se realizó dosis de azul de metileno al 1% por vía intravenosa, con franca mejoría clínica a la hora de la administración del antídoto y descenso de niveles de metahemoglobina. Alta médica a las 36 horas del ingreso. Existía una relación cronológica entre la exposición a nitratos por ingesta de un puré de acelgas y la aparición del cuadro. Los niveles de nitratos hallados en dicho alimento fueron muy elevados considerando estándares internacionales, lo que sumado a una inadecuada conservación del alimento cocido los días previos, permitió confirmar el planteo etiológico realizado. Resulta importante sospechar esta entidad patológica poco frecuente frente a cianosis que no mejora con oxígeno, y prevenir cuadros similares al descrito mediante una adecuada manipulación y conservación de las verduras con alto contenido en nitratos.


Methemoglobinemia is a clinical syndrome due to the presence of an aberrant form of hemoglobin, caused by various oxidizing agents. The study reports a case of severe methemoglobinemia associated with the ingestion of chard puree with high levels of nitrates and nitrites. A 1-year-old patient with a history of atrial septal defect (ASD), who progressively showed change of skin color 7 hours earlier, accompanied by vomiting. She was admitted to the Emergency Department with generalized cyanosis not improving with oxygen therapy, tachycardia and tendency to hypotension. In the intensive care unit, an echocardiogram showed ASD without hemodynamic complications. Methemoglobinemia 37%. A 1% methylene blue dose was administered intravenously, with clinical improvement one hour after antidote administration and decrease in methemoglobin levels. Medical discharge at 36 hours of admission. There was a chronological relationship between nitrates exposure by ingestion of chard puree and the clinical onset of methemoglobinemia. The toxic cause was confirmed after high nitrates levels were found in this vegetable considering international standards, and an inadequate preservation of the cooked chard on previous days. It is important to suspect this rare pathological entity when cyanosis fails to improve with oxygen, and to prevent poisonings similar to those described by an adequate manipulation and preservation of vegetables with high nitrate levels.


Subject(s)
Humans , Beta vulgaris/poisoning , Enzyme Inhibitors/therapeutic use , Methemoglobinemia , Methemoglobinemia/diagnosis , Methylene Blue/therapeutic use , Antidotes/therapeutic use , Nitrates/poisoning , Cyanosis/etiology , Foodborne Diseases , Foodborne Diseases/diagnosis , Methemoglobinemia/complications
5.
Biosci. j. (Online) ; 33(5): 1126-1133, sept./oct. 2017. tab, graf
Article in English | LILACS | ID: biblio-966275

ABSTRACT

The beet culture has great economic and food importance in the world, especially with respect to energy generation. In Brazil the culture is still little studied, lacking studies in all stages of production of culture, mainly in the management of fertilization. This study aimed to evaluate the effect of organo-mineral fertilization on growth, gas exchanges and production of beet, cv. Katrina. The experiment was conducted under field conditions in the municipality of Pombal-PB, Brazil, from September to December 2015, in a randomized block design. The treatments were arranged in a 4 x 2 factorial scheme and corresponded to four periods of fermentation of the biofertilizer (10, 20, 30 and 40 days) applied in soils with and without mineral fertilization, with four replicates and 18 plants evaluated per plot. Plants were conducted for 70 days after transplanting, in 15-cm-high, 1-m-wide beds. During this period, plant growth, gas exchanges and production components were evaluated. The application of mineral biofertilizer fermented for 20 to 30 days, associated with mineral fertilization with 36 g m-2 of P2O5, 18.0 g m-2 of K2O and 14 g m-2 of N at planting, promoted better performance of growth, gas exchanges and production of beet plants. In the soil without mineral fertilization, it is recommended to use the longest biofertilizer fermentation periods, 30 to 40 days, for beet cultivation.


A cultura da beterraba tem grande importância econômica e alimentar no mundo, especialmente no que diz respeito à geração de energia. No Brasil, a cultura ainda é pouco estudada, faltando estudos em todos os estágios de produção da cultura, principalmente no manejo da adubação. Objetivou-se no presente trabalho, avaliar a adubação organomineral no crescimento, trocas gasosas e produção da beterraba cv. Katrina. O experimento foi conduzido em condições de campo, no município de Pombal, PB, no período de setembro a dezembro de 2015. O delineamento experimental utilizado foi o de blocos ao acaso, com tratamentos arranjados em esquema fatorial 4 x 2, relativos a quatro períodos de fermentação do biofertilizante (10, 20, 30 e 40 dias) aplicados em solos com e sem adubação mineral, com quatro repetições e 18 plantas úteis por parcela. As plantas foram conduzidas durante 70 dias após o transplantio, em canteiros de 15 cm de altura, com 1 m de largura. Durante esse período, avaliou-se o crescimento, trocas gasosas e os componentes de produção. A adubação com biofertilizante mineral fermentado no período entre 20 e 30 dias associado à adubação mineral com 36 g m-2 de P2O5, 18,0 g m-2 de K2O e 14 g m-2 de N no plantio, proporcionou melhor desempenho no crescimento, trocas gasosas e produção da beterraba. No solo sem adubação mineral, recomenda-se a utilização dos maiores períodos de fermentação do biofertilizante, 30 a 40 dias, para o cultivo da beterraba.


Subject(s)
Agricultural Cultivation , Chenopodiaceae , Beta vulgaris/growth & development , Fertilizers
6.
Electron. j. biotechnol ; 27: 1-7, May. 2017. tab, ilus, graf
Article in English | LILACS | ID: biblio-1009846

ABSTRACT

Background: Genetic diversity studies are important for the selection of parents with a greater combination capacity that, when crossed, increase the chances of obtaining superior genotypes. Thus, 26 polymorphic simple sequence repeat (SSR) primers were used to assess the genetic diversity of 140 individual samples from 12 diploid sugar beet pollinators (pollen parents) and two cytoplasmic male sterile (cms) lines (seed parents). Eight pollinators originated from three research centers in the United States Department of Agriculture, while four pollinators and cms lines were from the Institute of Field and Vegetable Crops, Novi Sad, Serbia. Results: In total, 129 alleles were obtained, with a mean of 3.2 alleles per SSR marker. The observed heterozygosity ranged from 0.00 to 0.87 (mean = 0.30). Expected heterozygosity and Shannon's information index were the lowest for marker BQ590934 and the highest for markers SB15s and FDSB502s; the same markers were the most informative, with PIC values of 0.70 and 0.69, respectively. Three private alleles were found in pollinator EL0204; two in pollinator C51; and one in pollinators NS1, FC221, and C93035. Molecular variance showed that 77.34% of the total genetic variation was attributed to intrapopulation variability. Cluster and correspondence analysis grouped sugar beet pollinators according to the breeding centers, with few exceptions, which indicate that certain amount of germplasm was shared, although centers had their own breeding programs. Conclusions: The results indicate that this approach can improve the selection of pollinators as suitable parental components and could further be applied in sugar beet breeding programs.


Subject(s)
Pollen/genetics , Genetic Variation , Beta vulgaris/genetics , Polymorphism, Genetic , Seeds/genetics , Selection, Genetic , Breeding , Polymerase Chain Reaction , DNA, Plant/genetics , Microsatellite Repeats , Pollination , Genotype
7.
Mycobiology ; : 263-269, 2017.
Article in English | WPRIM | ID: wpr-729662

ABSTRACT

The genus Peronospora, an obligate biotrophic group belonging to Oomycota, causes serious damage to a variety of wild and ornamental plants, as well as cultivated crops, such as beet, rose, spinach, and tobacco. To investigate the diversity of Peronospora species parasitic to Stellaria and Pseudostellaria (Caryophyllaceae) plants in Korea, we performed a morphological analysis on dried herbarium specimens and molecular phylogenetic inferences based on internal transcribed spacer rDNA and cox2 mitochondrial DNA sequences. As a result, it was confirmed that there are four species of Peronospora parasitic to specific species of Stellaria and Pseudostellaria, all of which were hitherto unrecorded in Korea: P. alsinearum (ex Stellaria media), P. stellariae-aquaticae (ex Stellaria aquatica), P. stellariae-uliginosae (ex Stellaria alsine), and P. pseudostellariae (ex Pseudostellaria palibiniana). In addition, Peronospora specimens parasitic to Pseudostellaria davidii differed morphologically from P. pseudostellariae owing to the large and ellipsoidal conidia; this morphological discrepancy was also validated by the high genetic divergence between the two species. Peronospora casparyi sp. nov. is described and illustrated here.


Subject(s)
Beta vulgaris , Caryophyllaceae , Classification , DNA, Mitochondrial , DNA, Ribosomal , Host Specificity , Korea , Oomycetes , Peronospora , Phylogeny , Spinacia oleracea , Spores, Fungal , Stellaria , Tobacco
8.
São Paulo; s.n; s.n; 2017. 130 p. tab, graf, ilus.
Thesis in Portuguese | LILACS | ID: biblio-847739

ABSTRACT

Moléculas orgânicas fluorescentes são uma importante ferramenta para biologia celular. Compostos ideais para esta aplicação devem ter alto brilho (produto do coeficiente de atenuação molar e do rendimento quântico de fluorescência), ser fotoestáveis e internalizáveis, não comprometer a viabilidade celular e interagir com biomoléculas com algum grau de especificidade. Nesta Tese de Doutorado é apresentado o estudo do uso de cBeet120, uma betalaína cumarínica artificial, e células de glioma humano da linhagem U87-MG. Betalaínas são pigmentos de plantas que apresentam alta biocompatibilidade que servem como material de partida para o desenvolvimento de derivados funcionais. A sonda se acumula principalmente no núcleo das células U87- MG e marca principalmente nucléolos via interação com proteínas. A presença de DNAse ou RNAase elimina a marcação nuclear, sem afetar a fraca marcação citoplasmática de fundo. Estudos de inibição de transporte sugerem que cBeet120 é internalizada por transportadores de L-glutamato da família de transportadores de amino ácidos excitatórios (EAAT). O uso de artemisinina para inibição Ca2+-ATPases aumenta a velocidade de internalização de cBeet120 em células U87-MG. Quando irradiada com luz de cor ciano, cBeet120 no interior do núcleo de células vivas é fotoativada, resultando em um aumento da intensidade de fluorescência com o tempo (monitorado por 90 min) e o deslocamento hipsocrômico do máximo de emissão. Em células fixadas com paraformaldeído, o padrão de marcação da célula se torna mais difuso e a sonda emite fluorescência sem fotoativação. Medidas de tempo de vida de fluorescência em solução e imageamento por microscopia de tempo de vida de fluorescência permitem inferir a ocorrência da formação de um complexo proteína-cBeet120 ou um produto de transiminação que pode estar sujeito a isomerização cis/trans


Fluorescent organic molecules are an important tool for cell biology. Ideal compounds for this application must have high brightness (product of the molar attenuation coefficient and fluorescence quantum yield), be photostable and internalizable by cells, do not compromise cellular viability and interact with biomolecules with some degree of specificity. In this Doctorate Thesis, we describe the interaction of cBeet120, an artificial coumarinic betalain, and human glioma cells of line U87-MG. Betalains are plant pigments that exhibit high biocompatibility that serve as starting material for the development of functional derivatives. The probe accumulates mainly in the nucleus of the U87-MG cells and mainly marks nucleoli via interaction with proteins. The presence of DNAse or RNAase eliminates nuclear labeling, without affecting the poor background cytoplasmic labeling. Transport inhibition studies suggest that cBeet120 is internalized by L-glutamate transporters from the excitatory amino acid transporter (EAAT) family. The use of artemisinin for inhibition Ca2+-ATPases increases the rate of cBeet120 internalization in U87-MG cells. When irradiated with cyan colored light, cBeet120 within the nucleus of living cells is photoactivated, resulting in an increase in fluorescence intensity over time (monitored for 90 min) and the hypochromic shift of the emission maximum. In cells fixed with paraformaldehyde, the labeling pattern of the cell becomes more diffuse and the probe emits fluorescence without photoactivation. Fluorescence life-time measurements in solution and fluorescence life-time imaging microscopy allows to infer the occurrence of the formation of a protein-cBeet120 complex or the formation of a transimination product that may be subject to cis/trans isomerization


Subject(s)
Beta vulgaris/metabolism , Coumarins/analysis , Glioma/complications , Molecular Mechanisms of Pharmacological Action , Betalains , Fluorescence , Glioblastoma/complications
9.
Acta toxicol. argent ; 24(3): 180-186, dic. 2016. ilus, tab
Article in Spanish | LILACS | ID: biblio-837864

ABSTRACT

El Zineb es un plaguicida perteneciente a la familia de los etilenbisditiocarbamatos (EBDC) ampliamente utilizado en la provincia de Jujuy (Argentina). La toxicidad de este fungicida está dada por la etilentiourea (ETU) que es el principal producto de su degradación. La ETU tiene efectos mutagénicos, teratogénicos y cancerígenos en animales. La EPA la clasifica como probable cancerígeno humano. El objetivo del trabajo fue determinar la transformación del Zineb a ETU en acelgas cultivadas en Jujuy, por efecto del calor húmedo por tratamiento térmico. El método se basó en la extracción de la ETU con agua y su posterior reextracción con diclorometano a pH alcalino. El extracto se purificó por cromatografía en columna de alúmina y la ETU se cuantificó por HPLC con un detector UV-Visible a 232 nm. Para cumplir con el objetivo planteado se realizó un cultivo experimental de acelgas a las que se aplicó el fungicida Zineb, el cual luego se cuantificó a diferentes periodos de tiempo. La ETU se cuantificó con y sin aplicación de calor húmedo. La concentración de ETU en las acelgas sometidas a tratamiento térmico fue de 8,07 mg/kg y transcurridos 30 días disminuyó a 0,05 mg/kg, lo que representa una disminución de más del 99 % respecto a la concentración inicial. Simultáneamente, se determinó la concentración de Zineb en las acelgas y se obtuvo, inicialmente, 44 mg/kg y luego de 36 días la concentración de Zineb disminuyó a 3,83 mg/kg, lo que representa una disminución del 91 % respecto a la concentración del plaguicida al inicio. La detección de ETU en acelgas sometidas a tratamientos térmicos confirma la transformación del fungicida Zineb a un producto de degradación clasificado según el IARC en el grupo 3. Esto pone en discusión la reglamentación Argentina vigente, por cuanto al finalizar los tiempos de carencia no se evalúan la presencia de metabolitos o productos de degradación potencialmente tóxicos.


Zineb is a pesticide belonging to the family of ethylenebisdithiocarbamates (EBDC) widely used in the province of Jujuy, Argentina. The toxicity of this fungicide is given by the ethylenethiourea (ETU) which is the main product of degradation. The ETU has mutagenic, teratogenic and carcinogenic effects in animals. The EPA classifies it as a probable human carcinogen. The aim of the study was to determine the transformation of Zineb to ETU in cultivated chards in Jujuy, by treatment with humid heat. The method is based on extraction of ETU with water and subsequent extraction of the aqueous phase with dichloromethane. The extract was cleaned by alumina column chromatography and the ETU was quantified by HPLC with a UV-Visible detector at 232 nm. An experimental cultivation of chards was carried out and the Zineb fungicide was applied and then quantified at different time periods. The ETU was quantified with and without application of moist heat. The concentration of ETU in heat treated chards was 8.07 mg/kg and after 30 days decreased to 0.05 mg/kg, representing a decrease of more than 99 % over the initial concentration. Simultaneously, Zineb concentration in chard was initially determined as 44 mg/kg and, 36 days later, the Zineb concentration decreased to 3.83 mg/kg, representing a 91% decrease with respect to the concentration of the pesticide at the beginning. Detection of ETU in chards subjected to heat treatment confirms the transformation of the fungicide Zineb to a degradation product classified by the IARC as group 3. This puts into discussion the current Argentine regulations since, at the end of the deficiency times the presence of potentially toxic metabolites or degradation products is not evaluated.


Subject(s)
Humans , Beta vulgaris , Ethylenethiourea/toxicity , Thermic Treatment/adverse effects , Zineb/toxicity , Argentina/epidemiology , Biodegradation, Environmental
10.
Rev. cuba. plantas med ; 21(4)oct.-dic. 2016. graf, tab
Article in Spanish | LILACS, CUMED | ID: biblio-960658

ABSTRACT

Introducción: actualmente se ha demostrado una relación entre el consumo de alimentos con altos niveles de compuestos antioxidantes, los cuales están directamente asociados con la prevención de enfermedades. Objetivos: evaluar la actividad antioxidante de las pulpas Rubus glaucus B, Vaccinium floribundum K y Beta vulgaris L. Métodos: Rubus glaucus Benth variedad Castilla, Vaccinium floribundum K y Beta vulgaris L fueron adquiridas en un mercado local de la ciudad de Cartagena, Bolívar (10°25'25″N 75°31'31″O). La pulpa se obtuvo a partir del fruto (mora y agraz) y raíz (remolacha); y le se determinó pH, Brix, acidez titulable, índice de madurez, humedad, ceniza, grasa, proteínas, fibra cruda, carbohidratos y la actividad antioxidante fue determinada mediante la técnica de actividad antiradicalaria por el método DPPH, asimismo el contenido de fenoles totales se realizó por el método colorimétrico Folin-Ciocalteu. Resultados: los sólidos solubles totales oscilaron entre 5,94 - 10,91 Brix, pH 2,9 - 5,96, ceniza 0,407 - 1,05 g/100 g, humedad 87,263 - 83,95 g/100 g, proteína 0,624 - 1,59 g/100 g, grasa 0,12 - 0,62 g/100 g, fibra cruda 2,107 - 3,637 g/100 g, carbohidratos 10,01 - 14,446 g/100 g para las pulpas de mora, agraz y remolacha. Los resultados de la prueba de actividad antioxidante presentaron valores de IC50 mediante la técnica de DPPH• en el rango de 53,33 - 141,88 µg/mL, lo cual está directamente relacionado con el contenido en fenoles. Conclusiones: por lo tanto, la pulpa de Rubus glaucus Benth variedad Castilla, Vaccinium floribundum K y Beta vulgaris L, son considerados como promisorios para diseñar productos nutracéuticos por su elevada actividad antioxidante(AU)


Introduction: A direct relationship has been proved to exist between consumption of foodstuffs with a high content of antioxidant compounds and disease prevention. Objectives: Evaluate the antioxidant activity of pulps from Rubus glaucus B., Vaccinium floribundum K. and Beta vulgaris L. Methods: Rubus glaucus Benth variety Castile, Vaccinium floribundum K. and Beta vulgaris L. were acquired at a local marketplace in the city of Cartagena, Bolívar (10°25?25?N 75°31?31?W). The pulp was obtained from fruits (Andean raspberry and Andean blueberry) and roots (beet). Determination was conducted of its pH, Brix, titratable acidity, maturation index, humidity, ash, fat, proteins, crude fiber, carbohydrates, and antioxidant activity using the technique of anti-radical activity by the DPPH method. Content of total phenols was determined by the Folin-Ciocaltey colorimetric method. Results: Total soluble solids ranged between 5.94 - 10.91 Brix, pH 2.9 - 5.96, ash 0.407 - 1.05 g / 100 g, humidity 87.263 - 83.95 g / 100 g, protein 0.624 - 1.59 g / 100 g, fat 0.12 - 0.62 g / 100 g, crude fiber 2.107 - 3.637 g / 100 g, carbohydrates 10.01 - 14.446 g / 100 g for pulps from Andean raspberry, Andean blueberry and beet. Antioxidant activity testing using the DPPHo technique obtained CI50 values of 53.33 - 141.88 ?g/ml, directly proportional to phenol content. Conclusions: It is therefore concluded that pulp from Rubus glaucus Benth variety Castile, Vaccinium floribundum K. and Beta vulgaris L., is considered to be promising for the design of nutraceutical products due to its antioxidant activity(AU)


Subject(s)
Humans , Beta vulgaris , Blueberry Plants , Rubus , Antioxidants , Colombia
11.
Rev. bras. plantas med ; 18(2): 558-562, graf
Article in Portuguese | LILACS | ID: lil-787954

ABSTRACT

RESUMO O presente trabalho teve como objetivo principal avaliar o potencial do uso do óleo essencial de Aloysia citriodora no controle in vitro de Fusarium sp., isolado de plântulas de beterraba infectadas com o mesmo. O trabalho foi realizado por meio de dois experimentos: um sobre o efeito do óleo essencial no crescimento micelial, e outro sobre o efeito na germinação de conídios do fungo. No primeiro trabalho, avaliou-se em delineamento inteiramente ao acaso, o efeito das concentrações 0,0155%; 0,0315%; 0,0625%; 0,125%; 0,250% e 0,500% do óleo essencial de A. citriodora em placas de Petri® com meio de cultivo BDA, mais a testemunha, com meio BDA puro. Cada placa foi considerada uma repetição, as quais foram incubadas a 24ºC±1ºC e submetidas a fotoperíodo de doze horas. Avaliou-se o crescimento radial do patógeno em função do crescimento micelial do patógeno quando este atingia as bordas da primeira placa. No segundo experimento, as mesmas concentrações foram testadas, nas mesmas condições de incubação, no entanto, em lâminas de microscopia com meio BD. Utilizou-se delineamento inteiramente ao acaso, sendo considerada como unidade experimental cada lâmina utilizada. Avaliou-se 24 horas após a incubação, em microscópio óptico, a germinação dos primeiros vinte conídios visualizados a partir do canto esquerdo superior para o direito. Os resultados do segundo experimento foram expressos em porcentagem de germinação de conídios. Os resultados obtidos permitiram concluir que este óleo essencial possui efeito fungistático e fungicida sobre o crescimento micelial e na germinação de conídios de Fusarium sp.. Além disso este efeito é maior em função do aumento da concentração do óleo essencial.


ABSTRACT This study aimed to evaluate the potential use of Aloysia citriodora essential oilisolated from beet seedlings infected with it. The study was performed by conducting two experiments evaluating the effect of the essential oil on mycelial growth and fungus conidia germination. In the first study, in a completely randomized design, the effect concentrations (0.0155%; 0.0315%; 0.0625%; 0.125%; 0.250% and 0.500%) of essential oil of A. citriodorawas evaluated on Petri® dish with a PDA cultivation medium, plus the control, with half pure PDA. Each plate was taken as a repeat, and incubated at 24°C±1°C and a photoperiod of twelve hours. The radial growth of the pathogen, when the first plate was hit by the mycelial growth of the pathogen on its edges was evaluated. In the second experiment, the same concentrations were tested under the same incubationconditions, however, on microscope slides with half PD medium. The design was used completely randomized, each microscope slide used was considered as one experimental unit. Germination of the first 2 conidia strains, viewed from the upper left to the right was evaluated24 hours after incubation, using an optical microscope. The results of the second experiment were expressed as a percentage of conidia germination. The results obtained showed that this essential oil has fungistatic and fungicidal effect on the mycelial growth and at the conidia germination of Fusarium sp., which has a greater effect with increasing concentrations.


Subject(s)
Fusarium/classification , Lippia/classification , Oils, Volatile/analysis , Beta vulgaris/classification , Plants, Medicinal/classification , Verbenaceae/classification
12.
Rev. luna azul ; (42): 44-53, ene.-jun. 2016. ilus, tab
Article in Spanish | LILACS-Express | LILACS | ID: lil-791175

ABSTRACT

Dentro de las posibles formas de contaminación de las hortalizas con plomo se encuentran el suelo cultivado, el agua de riego y la atmósfera; esta última debido a la suspensión de las partículas resultantes de la combustión principalmente de hidrocarburos. Con el objetivo de lograr determinar la cantidad de plomo presente en Acelga común Beta vulgaris L. producida en el contexto de la agricultura urbana, se implementó una huerta con dicho sistema productivo en la ciudad, la cual se localiza en las coordenadas: Latitud 4°42'39,6036" y Longitud 74°5'46,6152", a menos de 500 metros de distancia de dos vías vehiculares principales de la ciudad de Bogotá. Previo a la siembra, se realizaron dos tipos de análisis: contenidos de plomo en suelo cultivado y en agua para riego, con la finalidad de obtener un diagnóstico preliminar sobre los contenidos de este metal pesado en estas dos posibles fuentes de contaminación. Las labores de sistema productivo relacionadas con prácticas culturales se realizaron de manera artesanal, por lo tanto no se realizó ningún tipo de fertilización y en el manejo de plagas no hubo intervención de ningún tipo de producto con la finalidad de evitar sesgos en el presente trabajo. Al momento de la cosecha, se muestreó al azar el 10% de la población total de plantas sembradas; esta muestra fue analizada por medio de espectrofotometría de absorción atómica según procedimiento del método oficial 968.08 de la AOAC, arrojando como resultado 0 ppm de plomo. Estos resultados permiten afirmar que la producción agrícola urbana es una alternativa que fomenta la seguridad alimentaria, no solo desde los aspectos de ingesta de macro y micronutrientes, sino también desde el punto de vista de calidad e inocuidad de los alimentos al no detectarse plomo en el tejido vegetal.


Cultivated soil, irrigation water and the atmosphere are among the possible forms of contamination with lead of vegetables, the latter due to suspension of the particles resulting from the combustion, mainly hydrocarbons. In order to be able to determine the levels of lead present in common Chard Beta vulgaris L. produced in the context of urban agriculture, a vegetable garden with this production system was implemented in the city, which is located at coordinates: Latitude 4°42'39.6036" and Longitude 74°5'46.6152", less than 500 meters away from two main vehicular roads in the city of Bogotá. Before sowing, two types of analysis were performed: lead content in cultivated soil and irrigation water, with the purpose of obtaining a preliminary diagnosis of the contents of this heavy metal in these two possible sources of contamination. The productive system work related to culture practices were made using traditional methods, therefore no fertilization was performed and there was no intervention of any type of product for pest management with the aim of avoiding bias in this study. At harvest time, 10% of the total of plants population planted was randomly selected; this sample was analyzed by atomic absorption spectrophotometry through the AOAC official method 968.08 yielding a result of 0 ppm of lead. These results allow the confirmation that urban farming is an alternative that promotes food security, not only from the intake of macro and micro nutrients aspect, but also from the of quality and food safety point of view, since for lead was not detect in the plant tissue.


Subject(s)
Humans , Urban Agriculture , Spectrophotometry , Beta vulgaris , Lead
13.
Rev. cuba. plantas med ; 21(1)jan.-mar. 2016. ilus, tab
Article in Portuguese | LILACS | ID: lil-781969

ABSTRACT

Introdução: Beta vulgaris spp. conhecida popularmente como beterraba é bastante utilizada, além do consumo alimentar, de maneira etnofarmacológica para o combate de diversas infecções como: dores no trato gastrointestinal, inflamações crônicas, lesões nas genitais, inflamações nos ovários, cólicas, problemas renais, problemas cardíacos e diabetes. Objetivo: avaliar a atividade antibacteriana e modulatória dos extratos metanólicos e hexânicos dos frutos de B. vulgaris frente a cepas bacterianas padrões e multirresistentes, além de determinar as principais classes de metabólitos secundários. Métodos: os extratos metanólicos e hexânicos de B. vulgaris foram analisados para a atividade antibacteriana por meio de teste de microdiluição em caldo para determinação de Concentração Inibitória Mínima e modulação de aminoglicosídeos a gentamicina e amicacina. Resultados: às cepas de Staphylococcus aureus e Escherichia coli diminuiram a Concentração Inibitória Mínima de 64 µg/mL and 256 µg/mL quando combinadas aos antibióticos e extratos, Apresentando, portanto um efeito de aumento da atividade antibiótica, com exceção para o extrato hexânico em associação com a gentamicina contra cepas multirresistentes de Staphylococcus aureus. Conclusão: na prospecção fitoquímica foram evidenciados a presença de vários metabólitos secundários, o que pode explicar a ação bactericida desta planta. Portanto, diante dos resultados, B.vulgaris é uma fonte promissora no combate a resistência bacteriana(AU)


Introducción: Beta vulgaris SSP. conocido em lo popular como remolacha, es muy utilizado, además como consumo de alimentos, de manera etnofarmacológica para combatir varias infecciones como: dolores en el tracto gastrointestinal, inflamaciones crónicas, lesiones genitales, inflamaciones en ovarios, cólicos, problemas renales, diabetes y problemas del corazón. Objetivo: evaluar la actividad anti-bacteriana y moduladora de extractos metanólicos y hexânicos del fruto de B. vulgaris frente de cepas bacterianas multirresistentes estándares y además de determinar los principales metabolitos secundarios. Métodos: los extractos metanólicos y hexânicos de B. vulgaris fueron analizados para la actividad antibacteriana mediante prueba de microdilución en caldo para la determinación de la Concentración Mínima Inhibitoria y la modulación de aminoglicósidos gentamicina y amikacina. Resultados: las cepas de Staphylococcus aureus y Escherichia coli disminuyeron la Concentración Mínima Inhibitoria de 64 µ g/mL y 256 µ g/mL al combinarlos con antibióticos y los extractos, mostrando así un aumento de la actividad antibiótica. Excepto el extracto hexânico en combinación con la gentamicina contra cepas multirresistentes de Staphylococcus aureus. La fitoquímica evidencia la presencia de varios metabolitos secundarios que pueden explicar la acción bactericida de esta planta. Conclusión: los resultados de B. vulgaris muestran que es una fuente prometedora en la lucha contra la resistencia bacteriana(AU)


Introduction: Beta vulgaris spp. is popularly known as beets, widely used in ethno pharmacological way to fight various infections of the gastrointestinal tract such as pain, chronic inflammation, and sores on the genitals, inflammation of the ovaries, cramps, kidney problems, heart problems and diabetes. Objective: to evaluate the antibacterial and modulatory activity of methanol extracts of fruits and hexanic of B. vulgaris against strains of multiresistant bacterial and standards. In addition, to determining the major classes of secondary metabolites such as flavonoids and tannins. Methods: Hexane and methanol extracts of B. vulgaris were analyzed to antibacterial activity by the broth microdilution test for determination of Minimum Inhibitory Concentration and modulation and aminoglycosides such as amikacin, gentamicin. Results: the strains relevant of Escherichia coli and Staphylococcos aureus decreased Minimum Inhibitory Concentration of 64 mg/mL and 256 mg/mL thereof when combined antibiotic and extracts. Showing, so a enhancement effect of antibiotic activity, except for the hexane extract in combination with gentamicin against multidrug-resistant strains of Staphylococcus aureus. Conclusion: in phytochemical were shown the presence of various secondary metabolites, which may explain the bactericidal action of this plant. Therefore, given the results, B. vulgaris spp. is a promising source in combat bacterial resistance(AU)


Subject(s)
Humans , Beta vulgaris/drug effects , Anti-Bacterial Agents/therapeutic use
14.
Chinese Journal of Biotechnology ; (12): 880-888, 2016.
Article in Chinese | WPRIM | ID: wpr-242290

ABSTRACT

Cellulosic ethanol, with the advantages of renewable resource, cleanliness and safety, is the mainstream of new energy development and has obtained extensive attention worldwide. In this review, the biological characteristics of beets were introduced, and then the superiority and application progress of beets and its by-product sugar beet pulp in the bioethanol production were stated. At last, cellulosic ethanol production coupled with the component separation and comprehensive utilization of beet pulp was proposed.


Subject(s)
Beta vulgaris , Chemistry , Biofuels , Cellulose , Chemistry , Ethanol , Chemistry
15.
Arch. latinoam. nutr ; 65(2): 71-78, June 2015. tab
Article in English | LILACS | ID: lil-752716

ABSTRACT

The Chilean National School Feeding Program (NSFP) delivers breakfast and lunch meals that supply 250 and 450 kcal, respectively, along the country. In the last decades, a significant increase of obesity has been observed in primary education children, and it involves risk factors of non-communicable diseases. The dietary intake of foods containing phenolic compounds (PC) exerts favorable effects on health by reducing risk factors of prevalent diseases. The aim of the study was to measure the PC content and antioxidant capacity (AC) [ORAC and DPPH] of meals provided by the NSFP in Quillota, Chile, in 2011. The PC supply of the whole meals served ranged from 362.7 to 1,730 mg GAE. The best breakfast foods include whole grain cookie (2.59±0.3 mg GAE/g), bread with avocado, quince jelly or strawberry jam (1.61±0.13 to 2.05±0.3 mg GAE/g); while the best lunch salads include beetroot, lettuce, and cabbage/fish (1.66±0.3 to 2.35±0.1 mg GAE/g), and main courses contain legumes, or mixed vegetables. The lowest PC contents were observed in pasta and rice preparations (p<0.05). Among desserts, the best source of PC is fruit (1.81±0.04 to 6.91±0.31 mg GAE/g). The correlation between PC and AC varied according to the type of meal. PC content and AC are additional criteria for selecting the best quality meals, in addition to the nutrients and energy content. The results support the recommendation to increase the supply of fruits and vegetable/legumes preparations and fruits instead of starchy foods to scholars.


El Programa de Alimentación Escolar (PAE) para enseñanza básica en Chile distribuye, en todo el país, desayunos y almuerzos que aportan 250 y 450 kcal, respectivamente. En las últimas décadas ha aumentado significativamente la obesidad en escolares, lo que induce factores de riesgo de enfermedades no transmisibles. La ingestión de alimentos que contienen polifenoles (PF) ejerce efectos beneficiosos al reducir factores de riesgo de enfermedades prevalentes. El objetivo del estudio fue determinar el contenido de PF y la capacidad antioxidante (CA) [ORAC y DPPH] de los alimentos entregados por el PAE en Quillota, Chile, en 2011. El aporte de PF en las raciones servidas fluctuó entre 362.7 y 1,730 mg EAG. El mejor desayuno contenía galletón con granos integrales (2.59±0.3 mg EAG/g), pan con palta, dulce de membrillo o de fresas (1.61±0.13 a 2.05±0.3 mg EAG /g) y el mejor almuerzo, ensaladas de remolacha, lechuga, o col/pescado (1.66±0.3 a 2.35±0.1 mg EAG /g), y un plato principal con leguminosas o vegetales mixtos. Los contenidos menores de PF se observaron en platos con pastas y arroz (p<0.05). Entre los postres, la mejor fuente de PF es la fruta (1.81±0.04 a 6.91±0.31 mg EAG/g). La correlación entre PF y CA fluctuó según el tipo de preparación. El contenido de PF y la CA son criterios de calidad adicionales al aporte de energía y nutrientes de las preparaciones. Los resultados apoyan la recomendación de aumentar el aporte a los escolares de frutas y vegetales/leguminosas en lugar de alimentos con alto contenido de almidones.


Subject(s)
Child , Humans , Antioxidants/analysis , Meals , Phenols/analysis , Schools , Breakfast , Beta vulgaris/chemistry , Brassica/chemistry , Chile , Dietary Services/standards , Food Quality , Food Services/standards , Food Supply/standards , Lunch , Malus/chemistry , Nutrition Policy
16.
Rev. bras. plantas med ; 17(4,supl.3): 1078-1082, 2015. graf
Article in Portuguese | LILACS | ID: lil-776593

ABSTRACT

RESUMO O óleo volátil da melaleuca (Melaleuca alternifolia Maiden & Betche, Cheel) possui atividade antimicrobiana podendo causar efeitos sobre as plantas. Avaliou-se a inibição do óleo em Cercospora beticolaSacc., e seu efeito no aumento da produção e qualidade de raízes de beterraba. As doses foram de 0,13; 0,67; 0,80 e 1,00% do óleo, além das testemunhas composta pelo meio de cultura Batata Dextrose Ágar (BDA) no experimento in vitro, e água no experimento in vivo. As plantas foram pulverizadas duas vezes por semana. O delineamento foi inteiramente casualizado, com 4 repetições, e as médias foram comparadas pelo teste Tukey a 5% de probabilidade. O índice de infecção das folhas foi determinado por escala diagramática além do peso e diâmetro das raízes. Os resultados de inibição do crescimento micelial para as doses do óleo foram 0; 56; 87; 83 e 99%, e os índices de infecção: 77,08; 35,62; 21,04; 19,37 e 20,00%, respectivamente, para a testemunha e as doses 0,13; 0,67; 0,80 e 1,00% do óleo. Somente na concentração de 0,80% o óleo proporcionou relação positiva entre o ganho de peso e o diâmetro das raízes. O óleo de Melaleuca foi eficaz no controle de C. beticola e, como consequência, houve produção de raízes de beterraba com melhor desenvolvimento.


ABSTRACT The volatile oil from Melaleuca (Melaleuca alternifolia Maiden & Betche Cheel.) has antimicrobial properties and can promote several effects on plant cultivation. The aim of this study was to evaluate the inhibition of the oil in Cercospora beticola Sacc. and if it favors the growth and development of beet root. The doses were 0.13, 0.67, 0.8 and 1% of oil, besides the control PDA (potato-dextrose-agar) in vitro (laboratory condition) and with water as treatment control in vivo (field conditions). The plants were sprayed twice a week. The treatments were completely randomized and the averages were compared using the Tukey test at 5%. The infection rate of leaves was measured by diagrammatic scale besides the weight and diameter of tubers. The inhibition results of the radial growth by oil treatments were 0; 56, 87, 83 and 99%, while the infection rate showed: 77.08, 35.62, 21.04, 19.37 and 20% respectively to the control and to the oil concentration of 0,13; 0,67; 0,80 e 1,00%. Only at concentration of 0.8% the tea tree oil showed a positive relationship between tuber´s weight and tuber´s diameter gains. It can be concluded that tea tree oil is effective to controlling C. beticola, and also promotes an increase on development in beet tubers.


Subject(s)
Beta vulgaris/analysis , Fungi/classification , Tea Tree Oil/analysis
17.
Iranian Journal of Veterinary Research. 2015; 16 (1): 25-30
in English | IMEMR | ID: emr-171836

ABSTRACT

The present experiment aimed at increasing orange peel and sugar beet pulp protein content through solid-state fermentation by Trichoderma reesei and Trichoderma viride. In vitro digestibility and changes in the chemical composition of the fermented products were determined after seven days of fungal cultivation using gas production tests. The cultivation of T. reesei and T. viride on orange peels decreased neutral detergent soluble content [P<0.01] and increased cellulose, hemicellulose and lignin contents [P<0.01]. Changes in fiber fractions were found to be more pronounced with T. viride. The cultivation of T. reesei and T. viride on sugar beet pulp increased neutral detergent soluble content [P<0.01] and decreased cellulose and hemicellulose contents [P<0.01]. These changes were more pronounced with T. reesei. The cultivation of T. reesei or T. viride on orange peels or sugar beet pulp increased crude protein content [P<0.01] compared with the unfermented materials; however, the increase was more pronounced for orange peels fermented with T. viride when corrected for weight loss [P<0.05]. After 24 and 48 h of incubation, significant decreases in cumulative gas production [P<0.01] were observed in fermented sugar beet pulp and orange peels compared with the unfermented materials. Fungal treatment of orange peels and sugar beet pulp reduced the digestibility of in vitro organic matter, metabolizable energy and average fermentation and gas production rates [P<0.01]. The data showed that seven days of solid-state fermentation of orange peels and sugar beet pulp by T. reesei or T. viride can increase their crude protein content


Subject(s)
Citrus sinensis/chemistry , Beta vulgaris , Fermentation , Carbohydrates
18.
Biosci. j. (Online) ; 30(1): 93-100, jan./feb. 2014. tab
Article in Portuguese | LILACS | ID: biblio-946969

ABSTRACT

Este trabalho apresenta resultados da composição centesimal, quantificação de fenólicos e atividade antioxidante de partes comestíveis não convencionais das olerícolas: cenoura, couve em folha, beterraba, repolho verde, brócolis e rabanete. A composição centesimal foi determinada de acordo com a metodologia proposta pelo Instituto Adolfo Lutz, sendo o teor de carboidratos totais obtido pela diferença das outras frações analisadas. O teor de compostos fenólicos foi obtido utilizando o reagente folin-ciocaulteal e curva de calibração com o reagente ácido gálico, enquanto que a atividade antioxidante foi realizada utilizando o reagente de DPPH (1,1­difenil-2-picrilidrazila). Ao nível de 99% de probabilidade, algumas amostras de partes não convencionalmente comestíveis (talos e folhas) apresentaram diferenças significativas em relação à composição centesimal, mas com teores próximos aos obtidos pela parte convencionalmente comestível do vegetal. Em relação à atividade antioxidade, todas as partes comestíveis não convencionais das olerícolas em estudo apresentaram propriedades antioxidantes, entretanto, a intensidade desta ação foi diferenciada entre elas, sendo que talos de brócolis e folhas e talos de beterraba não apresentaram diferenças significativas entre si.


This paper presents the centesimal composition, total phenolics quantification and antioxidant activity of edible parts of non-conventional vegetables: carrots, cabbage leaf, beetroot, green cabbage, broccoli and radish. The composition was determined according to the methodology proposed by the Institute Adolfo Lutz, and the total carbohydrate obtained from the difference of the other fractions analyzed. The content of phenolic compounds was obtained using the Folin-ciocaulteal and calibration curve with gallic acid, whereas the antioxidant activity was performed using the reagent DPPH (1,1-diphenyl-2-picrylhydrazyl). At the 99% level of probability, some samples showed significant differences in relation to centesimal composition, but at levels proximate to those obtained by conventional edible part of the plant. Regarding the antioxidant activity, all the edible parts of crops in unconventional samples showed antioxidant properties, however, the strength of action in different extent, and broccoli stalks and leaves and stalks of sugar beet showed no significant differences between them.


Subject(s)
Plants , Phenolic Compounds , Antioxidants , Brassica , Daucus carota , Beta vulgaris , Raphanus
19.
An. Fac. Med. (Perú) ; 75(1): 9-12, ene. 2014. graf
Article in Spanish | LILACS, LIPECS | ID: lil-721830

ABSTRACT

Introducción: La betarraga es una planta oriunda del Mediterráneo utilizada para fines alimenticios y medicinales. Contiene flavonoides. Objetivos: Determinar la influencia del consumo del extracto de Beta vulgaris var. cruenta en el incremento de leucocitos, en el ratón albino. Diseño: Estudio experimental. Lugar: Bioterio de la Institución Educativa 1182. Material biológico: Veinte ratones albinos de la cepa balb/c, machos, de peso promedio 24 g. Intervenciones: Se formó dos grupos, experimental y control, cada uno de diez ratones. Al grupo experimental se le administró vía oral extracto de Beta vulgaris var. cruenta en dosis de 250 mg/kg, volumen de 2 mL, cada cinco horas durante una semana, alternando con nutrientes, y al grupo control solo se le administró nutrientes. Principales medidas de resultados: Recuento de leucocitos. Resultados: Dentro del periodo de acondicionamiento, se observó un ligero incremento en la media de leucocitos del grupo control (3 681 ± 431,1) frente al grupo experimental (3 579 ± 473,5), cuya diferencia no fue significativa. Luego de administrar al grupo experimental el extracto de Beta vulgaris var. cruenta, alternando con sus nutrientes respectivos, se observó diferencia significativa de la media (7 961 ± 275,4) frente al grupo control (3 693 ± 414,7) (t student p<0,05). Conclusiones: En condiciones experimentales, el consumo del extracto de Beta vulgaris var. cruenta incrementó significativamente el número de leucocitos.


Background: Beetroot is a flavonoid-containing Mediterranean plant used for food and medicinal purposes. Objectives: To determine the influence of Beta vulgaris var. cruenta extract consumption in increasing albino mice leukocytes. Design: Experimental study. Setting: School Number 1182 bioterium. Biologic material: Twenty male Balb/c albino mice weighing 24 g average. Interventions: Two groups of ten mice each were formed; the experimental group received Beta vulgaris var. cruenta extract at 250 mg/kg oral doses, 2 mL volume every five hours for one week, alternating with nutrients; the control group received nutrients only. Main outcome measures: Leukocyte count. Results: During the conditioning period a slight increase in leukocytes in the control group (3 681 ± 431.1) was found compared to the experimental group (3 579 ± 473.5), but the difference was not significant. After administering Beta vulgaris var. cruenta extract alternating with nutrient media to the experimental group a significant difference in leukocytes (7 961 ± 275,4) was observed when compared with the control group (3 693 ± 414,7) (t student p<0,05). Conclusions: In experimental conditions, the consumption of Beta vulgaris var. cruenta extract significantly increased the number of leukocytes.


Subject(s)
Male , Animals , Mice , Beta vulgaris/blood , Cytokines , Immunity, Cellular , Immunity, Humoral , Immunomodulation , Leukocyte Count , Epidemiology, Experimental , Plant Extracts , Flavonoids
20.
Mycobiology ; : 385-390, 2014.
Article in English | WPRIM | ID: wpr-729764

ABSTRACT

The beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) is difficult to control using chemical insecticides because of the development of insecticide resistance. Several pest control agents are used to control the beet armyworm. Entomopathogenic fungi are one of the candidates for eco-friendly pest control instead of chemical control agents. In this study, among various entomopathogenic fungal strains isolated from soil two isolates were selected as high virulence pathogens against larva of beet armyworm. Control efficacy of fungal conidia was influenced by conidia concentration, temperature, and relative humidity (RH). The isolates Metarhizium anisopliae FT83 showed 100% cumulative mortality against second instar larvae of S. exigua 3 days after treatment at 1 x 10(7) conidia/mL and Paecilomyces fumosoroseus FG340 caused 100% mortality 6 days after treatment at 1 x 10(4) conidia/mL. Both M. anisopliae FT83 and P. fumosoroseus FG340 effectively controlled the moth at 20~30degrees C. M. anisopliae FT83 was significantly affected mortality by RH: mortality was 86.7% at 85% RH and 13.4% at 45% RH. P. fumosoroseus FG340 showed high mortality as 90% at 45% RH and 100% at 75% RH 6 days after conidia treatments. These results suggest that P. fumosoroseus FG340 and M. anisopliae FT83 have high potential to develop as a biocontrol agent against the beet armyworm.


Subject(s)
Beta vulgaris , Fungi , Humidity , Insecticide Resistance , Insecticides , Larva , Metarhizium , Mortality , Moths , Paecilomyces , Pest Control , Soil , Spodoptera , Spores, Fungal , Virulence
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