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1.
Rev. ABENO ; 21(1): 1255, dez. 2021. tab, graf
Article in Portuguese | LILACS, BBO | ID: biblio-1371185

ABSTRACT

Oobjetivo desteestudofoianalisaro perfil dos usuários do Biobanco de Dentes Humanos da Universidade Federal do Paraná (BDH-UFPR), os serviços mais procurados, se háutilização de dentes obtidos externamentee o grau de satisfaçãodos usuários.Para isso,foi aplicado um questionário eletrônico semiestruturado à comunidade interna do curso de Odontologia daUFPR, entre agosto edezembro de 2019. Um total de 300 questionários foram respondidos, sendo263 por estudantes degraduação, 4 por pós-graduandos, 24 por professores e 9 por servidores técnico-administrativos.A maioria dos estudantes erado sexo feminino entre 17e 22 anos, enquantopara osservidores (professores e técnicos) houve uma distribuição semelhante entre os sexosna faixa etária entre 40e 59 anos. Embora 80,99% dos discentes afirmaram conhecer oBDH-UFPR, apenas 50,19%usaram seus serviçose59,32% relataram játerembuscadodentesexternamente.O serviço mais utilizado foi o empréstimo de dentes para atividades de ensino, porém apenas 42,15% os devolveram após ouso.Os dentes obtidos externamente foram provenientes,principalmente, deconsultórios particulares e apenas 56,33% dos alunosrelataram sempre ter submetido-os à desinfecção/esterilização, sendo a autoclave o método mais indicado. Todos os servidores conheciam o BDH-UFPR e,dentre os professores,a maioria realizoutanto empréstimos quanto doações. Os empréstimostiveram como principal finalidade a pesquisae 63,6% relataramterem devolvido os dentes.Quanto à satisfação, a comunidade acadêmica mostrou-se predominantemente totalmente satisfeita.Por fim, ressalta-se a necessidade de ampliar as ações de conscientização para a comunidade acadêmica quanto aos aspectos éticos, legais e de biossegurança na aquisição e manipulação de dentes humanos (AU).


Thisstudy aimed toanalyze the profile of users of the Human Teeth Biobank at the Federal University of Paraná (HTB-UFPR), the most sought-after services, whether externally obtained teeth are used and the degree of user satisfaction. For this, a semi-structured electronic questionnaire was applied to the internal community of the Dentistry course at UFPR, between August and December 2019. A total of 300 questionnaires were answered, 263 by undergraduate students, 4 by graduate students, 24 by professors and 9 by technical-administrative employees. Most students were female between 17 and 22 years old, while for employees(teachers and technicians) there was a similar distribution between the sexes in the age group between 40 and 59 years old. Although 80.99% of the students claimed to know the HTB-UFPR, only 50.19% used its services and 59.32% reported having already looked for teeth externally. The most used service was the withdrawalfor teaching activities, but only 42.15% returned them after use. The teeth obtained externally came mainly from private offices and only 56.33% of the students reported having always submitted them to disinfection/sterilization, with the autoclave being the most indicated method. All employees knew the HTB-UFPR and, among the teachers, most made both withdrawaland donations. The main purpose of the withdrawals was the research and 63.6% reported having returned their teeth. As for satisfaction, the academic community was predominantly fully satisfied. Finally, it emphasizes the need to expand awareness actions for the academic community regarding ethical, legal and biosafety aspects in the acquisition and manipulation of human teeth (AU).


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Personal Satisfaction , Tooth/anatomy & histology , Containment of Biohazards/ethics , Education, Dental/methods , Ethics, Dental/education , Health Knowledge, Attitudes, Practice , Surveys and Questionnaires/statistics & numerical data , Data Interpretation, Statistical , Biological Specimen Banks/ethics
2.
Ciênc. Saúde Colet. (Impr.) ; 26(9): 4321-4339, set. 2021. tab, graf
Article in English, Spanish | LILACS | ID: biblio-1339609

ABSTRACT

Resumen Los biobancos son infraestructuras multidisciplinares y esta investigación integradora pretende exponer su concepto a las diferentes ciencias que lo construyen e interpretan, para entender sus elementos esenciales de forma holística. La revisión integradora se realizó siguiendo la guía PRISMA y la evaluación de la calidad según CASPe dando como resultado un total de 30 trabajos. El análisis de los datos se realizó a través de las categorías aristotélicas y los resultados se interpretaron según el paradigma de la complejidad de Edgar Morin. El concepto de Biobanco fue aclarado al considerarlo como la representación de un fenómeno bio-socio-cultural en el que los campos científicos desarrollan relaciones de tipo: complementarias, antagónicas y ambiguas de conocimientos y prácticas. Esta red de significación, desde la filosofía, impacta en la construcción de la subjetividad y en las formas de socialización.


Abstract Biobanks are multidisciplinary infrastructures and, accordingly, this integrative research seeks to bring out the concept of biobank in the various sciences that construct and interpret it, so as to arrive at a holistic understanding of its essential components. This integrative review - guided by PRISMA and with quality assessment following CASPe - resulted in a selection of 30 articles. Data were analysed by Aristotelian categories and the results were interpreted on the complexity paradigm of Edgar Morin. The biobank concept was clarified by considering it to be the representation of a biological, social and cultural phenomenon in which knowledge and practices from diverse scientific fields enter into complementary, antagonistic and ambiguous types of relationship. This network of signification, analysed here using categories from Aristotelian philosophy, has impacts on the construction of subjectivity and forms of socialisation.


Subject(s)
Humans , Philosophy , Biological Specimen Banks
4.
Actual. SIDA. infectol ; 29(105): 49-57, 2021 mar. fif, graf
Article in Spanish | LILACS, BINACIS | ID: biblio-1348938

ABSTRACT

La pandemia de COVID-19 ha puesto en jaque a los sistemas de salud en el mundo; la vinculación entre la investigación biomédica y la práctica asistencial ha probado ser un requisito fundamental para dar respuesta a la misma de manera eficiente y rápida. En este sentido, los biobancos se constituyen como un componente clave ya que favorecen el almacenamiento de grandes volúmenes de muestras biológicas gestionadas en base a criterios que garanticen su óptima calidad, armonización y seguridad, respetando requisitos éticos y legales que aseguran los derechos de los ciudadanos. La cesión de estas muestras a distintos grupos de investigación promueve el desarrollo de nuevas herramientas diagnósticas y terapéuticas y vacunas. Frente a la llegada del SARS-CoV-2 a la Argentina, el Biobanco de Enfermedades Infecciosas estableció rápidamente la colección COVID-19 constituida por muestras de plasma, suero y células mononucleares de sangre periférica de personas cursando la enfermedad o recuperadas. En solo seis meses se enrolaron 825 donantes, lo que significa alrededor de 14.000 viales de material biológico almacenados y a disposición de los investigadores que lo soliciten. A tal efecto, se realizaron seis actos de cesión a diversos grupos pertenecientes a instituciones de investigación, mientras que tres se encuentran en evaluación. Las muestras cedidas han permitido, por ejemplo, el desarrollo de kits serológicos de producción nacional; lo que pone de manifiesto que el rápido establecimiento de esta colección, bajo un sistema de gestión eficiente, constituye una herramienta muy valiosa en la respuesta a esta nueva enfermedad


The COVID-19 pandemic has driven an unprecedented health crisis. Cooperation between biomedical research and healthcare practice has been shown to be a fundamental requirement to provide an efficient and timely response. In this regard, biobanks are key components since they allow the storage of large volumes of biological samples with guaranteed optimum quality, harmonization and safety, ensuring ethical and legal requirements which protect citizen rights. The transfer of these samples to different research groups fosters the development of new diagnostic and therapeutic tools as well as vaccines. Upon SARS-CoV-2 arrival to Argentina, the Biobank of Infectious Diseases rapidly established the COVID-19 collection comprised by plasma, serum and peripheral blood mononuclear cells samples obtained from people within the acute phase of the infection or who have already recovered. In only 6 months, 825 donors were enrolled, representing around 14,000 vials of biological material stored and available to researchers who might require it. In this line, 6 transfer agreements have been already performed to different groups belonging to national research institutions, while 3 are under evaluation. The transferred samples have allowed, for instance, the development of nationally produced serologic kits, which shows that the rapid establishment of this collection, under an efficient management system, represents a highly valuable tool in the response to this new disease.


Subject(s)
Humans , Health Profile , Biological Specimen Banks/organization & administration , Health System Financing , COVID-19 , Health Services Accessibility , Informed Consent
5.
Article in Chinese | WPRIM | ID: wpr-888217

ABSTRACT

UK Biobank (UKB) is a forward-looking epidemiological project with over 500, 000 people aged 40 to 69, whose image extension project plans to re-invite 100, 000 participants from UKB to perform multimodal brain magnetic resonance imaging. Large-scale multimodal neuroimaging combined with large amounts of phenotypic and genetic data provides great resources to conduct brain health-related research. This article provides an in-depth overview of UKB in the field of neuroimaging. Firstly, neuroimage collection and imaging-derived phenotypes are summarized. Secondly, typical studies of UKB in neuroimaging areas are introduced, which include cardiovascular risk factors, regulatory factors, brain age prediction, normality, successful and morbid brain aging, environmental and genetic factors, cognitive ability and gender. Lastly, the open challenges and future directions of UKB are discussed. This article has the potential to open up a new research field for the prevention and treatment of neurological diseases.


Subject(s)
Biological Specimen Banks , Brain , Neuroimaging , United Kingdom
6.
Article in Chinese | WPRIM | ID: wpr-922717

ABSTRACT

To investigate whether the laboratory specimens preserved in Beijing Hospital Biobank during a specific period had been contaminated by SARS-Cov-2 through a cross-sectional study, and to establish a retrospective biobank safety screening system. Laboratory specimens were collected from the Department of Respiratory and Critical Care Medicine and the Fever Clinic of Beijing Hospital from November 1, 2019 to January 22, 2020, nucleic acid and serological antibody testing were performed for SARS-CoV-2 in these specimens (including 79 serum, 20 urine, 42 feces and 21 bronchoalveolar lavage fluid specimens). The safety of the stored samples during this period was defined by negative and positive results. Both the nucleic acid test and serological antibody test showed negative for SARS-CoV-2, indicating that these specimens were safely stored in the biobank. High-risk specimens collected in our hospital during the early stage of the COVID-19 outbreak are free of SARS-CoV-2, and a safety screening strategy for the clinical biobank is established to ensure the biosafety of these samples.


Subject(s)
Biological Specimen Banks , COVID-19 , Cross-Sectional Studies , Hospitals , Humans , Retrospective Studies , SARS-CoV-2
7.
Aliança do Tocantins; [s.n]; 31 mar. 2020. 24 p.
Non-conventional in Portuguese | LILACS, ColecionaSUS, CONASS, SES-TO | ID: biblio-1122750

ABSTRACT

Descreve ações de Vigilância e Atenção em Saúde do Município de Aliança do Tocantins, a serem executadas na rotina das Unidades de Saúde e fluxograma de atendimento frente a casos suspeitos de Infecção Humana pelo Novo CoronaVírus (COVID-2019). Busca minimizar riscos à população frente a casos suspeitos de COVID-2019. Divulga informações em Saúde e estabelece estratégia de Comunicação de Risco além de orientar a adoção de medidas preventivas e indicação de uso de Equipamento de proteção individual ­ EPI.


Describes Health Surveillance and Attention actions in the municipality of Aliança do Tocantins, to be carried out in the routine of the Health Units and flowchart of care in the face of suspected cases of Human Infection by the New CoronaVirus (COVID-2019). It seeks to minimize risks to the population in the face of suspected cases of COVID-2019. Discloses information on Health and establishes a Risk Communication strategy in addition to guiding the adoption of preventive measures and the indication of the use of Personal Protective Equipment - PPE.


Describe las acciones de Vigilancia y Atención en Salud en el municipio de Aliança do Tocantins, a realizarse en la rutina de las Unidades de Salud y diagrama de flujo de atención ante casos sospechosos de Infección Humana por el Nuevo CoronaVirus (COVID-2019). Busca minimizar riesgos a la población ante casos sospechosos de COVID-2019. Divulga información sobre Salud y establece una estrategia de Comunicación de Riesgos además de orientar la adopción de medidas preventivas y la indicación del uso de Equipos de Protección Individual - EPI.


Il décrit les actions de surveillance et de soins de santé dans la municipalité d'Aliança do Tocantins, à réaliser dans la routine des unités de santé et le diagramme de flux de soins pour les cas suspects d'infection humaine par le nouveau coronavirus (COVID-2019). Il cherche à minimiser les risques pour la population face aux cas suspects de COVID-2019. Diffuse des informations sur la santé et établit une stratégie de communication des risques en plus de guider l'adoption de mesures préventives et l'indication de l'utilisation des équipements de protection individuelle - EPI.


Subject(s)
Humans , Coronavirus Infections/prevention & control , Contingency Plans , Primary Health Care/organization & administration , Coronavirus Infections/transmission , Biological Specimen Banks/standards , Clinical Laboratory Techniques/methods , Infectious Disease Incubation Period , Clinical Laboratory Services/supply & distribution
8.
Washington; Organización Panamericana de la Salud; feb. 3, 2020. 10 p.
Non-conventional in Spanish | LILACS | ID: biblio-1096509

ABSTRACT

En enero de 2020, el agente etiológico responsable de un grupo de casos de neumonía grave en Wuhan, China, fue identificado como un nuevo betacoronavirus (2019-nCoV), distinto del SARS-CoV y MERS-CoV (1) (2) (3). La secuencia genómica completa de este nuevo agente está disponible y se han desarrollado diferentes protocolos de detección, aunque aún no se han validado por completo. Sin embargo, a la luz de la posible introducción de un caso sospechoso relacionado con el 2019-nCoV en la Región de las Américas, la Organización Panamericana de la Salud / Organización Mundial de la Salud (OPS / OMS) recomienda a los Estados Miembros garantizar su identificación oportuna, el envío de las muestras a laboratorios Nacionales o de referencia y la implementación del protocolo de detección molecular para 2019-nCoV, según la capacidad del laboratorio.


Subject(s)
Pneumonia, Viral/virology , Coronavirus Infections/ethnology , Coronavirus Infections/prevention & control , Coronavirus Infections/epidemiology , Biological Specimen Banks/supply & distribution , Containment of Biohazards/methods , Pandemics/prevention & control , Betacoronavirus/isolation & purification , China/epidemiology
12.
Rev. argent. microbiol ; 51(3): 214-220, set. 2019. tab
Article in English | LILACS | ID: biblio-1041827

ABSTRACT

Reference fungal cultures (RFCs) are essential for the internal quality control of laboratories. The production of these cultures requires standardized procedures (IRAM 14950:2016 and ISO 17034:2016 standards) carried out by a recognized and accredited laboratory. The aim of this work was to produce RFC in paper disks of autochthonous strains, characterized by two, homogeneous and stable reference methods traceable at species level. RFC were produced using 14 regional species (7 yeasts and 7 filamentous fungi) from the fungal culture collection (DMic). Paper disks were impregnated with a culture suspension, dried and packed. Homogeneity, viability, identity and purity were verified. Short-and long-term stability at different temperatures and storage times were studied. Characterization of each strain allowed to confirm its identity and to ensure its traceability at international level. Produced batches were homogeneous and stable at -20 ±5 °C for 30 months. This method of production was adequate to produce homogeneous and stable RFC with phenotypic and genotypic characteristics correctly defined and internationally traceable. Standardized procedures were developed for the production of certified RFC that could be transferred to other microorganisms. Providing RFC that represent regional strains allows laboratories to produce more reliable results with a favorable impact on medical diagnosis, the environment or the food industry.


Los cultivos microbianos de referencia (CR) son imprescindibles para el control de calidad interno de los laboratorios. Asegurar su producción requiere de procedimientos estandarizados (IRAM 14950:2016 e ISO 17034:2016) realizados en un laboratorio reconocido y acreditado. El objetivo de este estudio fue producir cultivos fúngicos de referencia en discos de papel, a partir de un panel de cultivos autóctonos caracterizados por dos métodos de referencia, trazables a nivel taxonómico de especie, homogéneos y estables. Se produjeron CR de 14 especies circulantes en Argentina (7 de levaduras y 7 de hongos miceliales), depositadas en la colección de hongos de interés médico (DMic). Los discos de papel fueron embebidos con una suspensión del cultivo por producir, secados y envasados. Se verificó la homogeneidad, viabilidad, identidad y pureza de cada lote. Se evaluó la estabilidad a corto y largo plazo a distintas temperaturas y tiempos de almacenamiento. La caracterización de cada CR nos permitió confirmar su identidad y asegurar su trazabilidad a nivel internacional. Los lotes producidos fueron homogéneos y estables durante 30 meses conservados a -20 ±5 °C. Este método resultó adecuado para producir CR homogéneos y estables, con características fenotípicas y genotípicas correctamente definidas y trazables a nivel internacional. Los procedimientos estandarizados desarrollados en este trabajo pueden ser transferidos para producir CR certificados de otros microorganismos. La provisión de CR que represente cepas regionales permite a los laboratorios producir resultados más confiables con un impacto favorable en el diagnóstico médico, los estudios ambientales y la industria alimenticia.


Subject(s)
Biological Specimen Banks , Fungi , Mycology/standards , Preservation, Biological/instrumentation , Preservation, Biological/methods , Quality Control , Reference Standards , Yeasts , Culture Media , Mycology/methods
13.
Rev. argent. microbiol ; 51(3): 221-228, set. 2019. ilus, tab
Article in English | LILACS | ID: biblio-1041828

ABSTRACT

The objective of this study was to identify twelve Brucella abortus isolates of bovine origin from the department of Nariño in Colombia up to the biovar level. These isolates are included in the collection of the Germplasm Bank of Microorganisms of Animal Health Interest -Bacteria and Virus (BGSA-BV). The identification was carried out through conventional methods such as macro and microscopic morphological descriptions, enzymatic activity, biochemical profile, substrate use and sensitivity to dyes. Complementary genotypic characterization was carried out using multiplex PCR for B. abortus, Brucella melitensis, Brucella ovis, and Brucella suis-Erytritol (AMOS-ERY-PCR), RFLP-IS711, by southern blot hybridization, as well as by the multiple locus variable number of tandem repeat analysis (MLVA) using the ery gene and the insertion sequence IS711 and variable number of tandem repeats (VNTR) as molecular markers. The results of the phenotypic and molecular characterization allowed to identify twelve isolates as B. abortus biovar 4 as well as to differentiate field from vaccine strains. This is the first study on the phenotypic and molecular identification of B. abortus isolates in Colombia. It was concluded that the phenotypic and molecular identification of twelve isolates as B. abortus biovar 4 could be achieved using conventional and molecular techniques with enough resolution power. The identification of these isolates to the biovar level in taxonomic and epidemiological terms will allow the use of this genetic resource as reference strains in future research. This finding constitutes the basis for identifying biotypes not previously reported in the country that might be useful to support brucellosis survey programs in Colombia.


El objetivo de este estudio fue identificar 12 aislamientos de Brucella abortus de origen bovino procedentes del departamento de Narino, Colombia, hasta la descripción de biovar. Estos aislamientos conforman la colección del Banco de Germoplasma de Microorganismos de Interés en Salud Animal, Bacterias y Virus. La identificación se hizo mediante métodos convencionales, como la descripción morfológica macro y microscópica de actividad enzimática, de perfiles bioquímicos, de utilización de sustratos y de sensibilidad a colorantes. Se hizo una caracterización genotipica complementaria mediante PCR múltiple para Brucella abortus, Brucella melitensis, Brucella ovisy Brucella suis-eritritol (AMOS-ERY-PCR); RFLP-/S7II; hibridación Southern blot y análisis multi-locus de repeticiones en tándem de número variable (MLVA), empleando como marcadores moleculares el gen ery, la secuencia de inserción /S711 y el número variable de repeticiones en tándem (VNTR). Los resultados de la caracterización fenotípica y molecular permitieron identificar 12 aislamientos de campo como B. abortus biovar 4 y diferenciar cepas de campo de cepas vacunales. Este es el primer estudio de identificación fenotípica y molecular de aislamientos de B. abortus en Colombia. Por su importancia taxonómica y epidemiológica, la identificación de estos aislamientos hasta el nivel de biovar permitirá disponer de recursos genéticos que se pueden emplear como cepas de referencia en futuras investigaciones. Estos resultados pueden considerarse como una base para la identificación de biotipos no reportados en el país y podrán ser utilizados en programas de monitoreo y vigilancia de la brucelosis bovina en Colombia.


Subject(s)
Animals , Cattle , Brucella abortus/isolation & purification , Brucellosis, Bovine/microbiology , Phenotype , Brucella abortus/classification , Brucella abortus/genetics , Brucella abortus/ultrastructure , Brucellosis, Bovine/epidemiology , DNA, Bacterial/genetics , Biomarkers , Bacteriological Techniques , Colombia/epidemiology , Biological Specimen Banks , Minisatellite Repeats , Multiplex Polymerase Chain Reaction , Genes, Bacterial , Genotype
14.
Rev. argent. salud publica ; 10(40): 51-54, 30 de septiembre 2019.
Article in Spanish | LILACS, BINACIS, ARGMSAL | ID: biblio-1024949

ABSTRACT

Los biobancos que almacenan muestras biológicas humanas y datos asociados de los donantes se han constituido en una herramienta esencial para la investigación biomédica. Su valor radica en el intercambio de las grandes cantidades de muestras y datos que almacenan para la realización de múltiples investigaciones futuras. Este nuevo escenario presenta desafíos éticos, legales y sociales relacionados con la protección de los derechos e intereses de los donantes y de la comunidad a la que pertenecen. En respuesta a estos desafíos y con el fin de promover en Argentina la existencia de biobancos con muestras de alta calidad para la realización de investigaciones con valor científico y social, que respeten en todo momento los derechos e intereses de los donantes y de la comunidad, la Secretaría de Gobierno de Salud ha elaborado una Guía de pautas éticas y legales para los biobancos con fines de investigación. Este artículo describe y analiza los puntos clave de la Guía


Subject(s)
Biological Specimen Banks , Confidentiality , Ethics, Research
15.
Rev. méd. Chile ; 147(7): 901-909, jul. 2019. tab, graf
Article in Spanish | LILACS | ID: biblio-1058620

ABSTRACT

The concept "Biobank" is relatively new in the scientific literature, and is not yet consensually defined, even for the World Health Organization (WHO). However, the use of human samples in biomedical research is a very old activity. The organized development of Biobanks in different places has grown in the last decade. The experience in different countries and continents has been diverse. In this special article we intend to summarize, organize and communicate to the national medical and scientific community, (i) the concept of Biobank, (ii) the international experience and a map of the Research Biobanks working in Chile, (iii) the basic biomedical and essential operational aspects to manage a Biobank for Research and (iv) the impact of a National Network of Biobanks implementation in the Chilean Health System. Ethical and regulatory aspects will not be included, given their intrinsic complexity, which should be discussed elsewhere.


Subject(s)
Humans , Biological Specimen Banks/organization & administration , Biomedical Research , Chile , Biological Specimen Banks/standards
17.
Mem. Inst. Oswaldo Cruz ; 114: e180432, 2019. tab, graf
Article in English | LILACS | ID: biblio-984761

ABSTRACT

BACKGROUND The Zika virus (ZIKV) epidemics that affected South America in 2016 raised several research questions and prompted an increase in studies in the field. The transient and low viraemia observed in the course of ZIKV infection is a challenge for viral isolation from patient serum, which leads to many laboratories around the world sharing viral strains for their studies. C6/36 cells derived from Aedes albopictus larvae are commonly used for arbovirus isolation from clinical samples and for the preparation of viral stocks. OBJECTIVES Here, we report the contamination of two widely used ZIKV strains by Brevidensovirus, here designated as mosquito densovirus (MDV). METHODS Molecular and immunological techniques were used to analyse the MDV contamination of ZIKV stocks. Also, virus passages in mammalian cell line and infecting susceptible mice were used to MDV clearance from ZIKV stocks. FINDINGS MDV contamination was confirmed by molecular and immunological techniques and likely originated from C6/36 cultures commonly used to grow viral stocks. We applied two protocols that successfully eliminated MDV contamination from ZIKV stocks, and these protocols can be widely applied in the field. As MDV does not infect vertebrate cells, we performed serial passages of contaminated stocks using a mammalian cell line and infecting susceptible mice prior to re-isolating ZIKV from the animals' blood serum. MDV elimination was confirmed with immunostaining, polymerase chain reaction (PCR), and analysis of the mosquitoes that were allowed to feed on the infected mice. MAIN CONCLUSIONS Since the putative impact of viral contaminants in ZIKV strains generally used for research purposes is unknown, researchers working in the field must be aware of potential contaminants and test viral stocks to certify sample purity.


Subject(s)
Humans , Animals , Virus Cultivation , Biological Specimen Banks , Zika Virus , DNA, Viral , Fluorescent Antibody Technique , Densovirus/genetics , Mice
18.
São Paulo; s.n; 2019. 85 p. ilust, quadros.
Thesis in Portuguese | LILACS, Inca | ID: biblio-1049749

ABSTRACT

O perfil de expressão gênica tem passado do cenário da pesquisa básica para a prática clínica, sendo cada vez mais utilizado como ferramenta na classificação de subtipos moleculares do câncer. No entanto, deve-se ter cautela ao interpretar as assinaturas gênicas, uma vez que os métodos de manuseio e preservação da amostra podem afetar a expressão gênica. "Isquemia fria", quando aplicada à coleta e preservação de tecidos para pesquisa, refere-se ao período transcorrido desde a retirada do órgão do corpo e coleta da amostra, até o momento do seu congelamento em nitrogênio líquido. O objetivo geral deste trabalho foi avaliar o impacto do tempo de isquemia fria na expressão gênica global pela técnica de microarray em um modelo animal. Avaliamos 3 órgãos (pulmão, fígado e rim) de 52 camundongos (Mus musculus C57Bl/6), gerando 312 tecidos submetidos a diferentes tempos de isquemia (zero ou referência, 15, 30, 45 e 60 minutos). A expressão gênica global foi avaliada na plataforma SurePrint G3 Mouse GE 8x60K Microarray, que cobre o genoma completo do camundongo. Como resultado deste trabalho, os RNA totais extraídos tiveram alta qualidade (RIN médio de 9,4) e observamos alterações em genes que podem ser atribuídas ao processo isquêmico per se (p<0.05 e fold-change |2|). Alguns desses genes são conhecidamente relacionados ao câncer: fatores de transcrição (Fos, Hif3A), oncogenes (Ret, Srsf3), supressores tumorais (Btg1, Hnf1a), genes envolvidos no reparo do DNA, diferenciação e atividades de quinase. Esses genes devem ser olhados com cautela nas assinaturas genômicas para um desempenho analítico mais confiável. Foram encontradas variações de expressão gênica relacionadas a processos de controle de íons intracelulares e controle do pH. Evidenciou-se a importância da criopreservação imediata do tecido, ou, pelo menos, o mais rápido possível após a coleta, visando minimizar os efeitos de variação da expressão gênica decorrentes da isquemia (AU)


Gene expression profile has been moved from basic research to clinical practice, and it is increasingly being used as a tool in the classification of molecular subtypes in cancer. However, caution should be exercised when interpreting gene signatures, as sample handling and preservation methods may affect gene expression. "Cold ischemia", when applied to tissue collection and preservation for research, refers to the period from organ removal from the body and sample collection until it is frozen in liquid nitrogen. The general objective of this work was to evaluate the impact of cold ischemia time on global gene expression by microarray technique in an animal model. We evaluated 3 organs (lung, liver and kidney) from 52 mice (Mus musculus C57Bl / 6), generating 312 tissues submitted to different ischemia times (zero or reference, 15, 30, 45 and 60 minutes). Global gene expression was evaluated on the SurePrint G3 Mouse GE 8x60K Microarray platform that covers the complete mouse genome. As a result of this work, the extracted total RNAs had high quality (mean RIN of 9.4) and we evidenced changes in genes that can be attributed to the ischemic process per se (p <0.05 and fold-change | 2 |). Some of these genes are known to be related to cancer: transcription factors (Fos, Hif3A), oncogenes (Ret, Srsf3), tumor suppressors (Btg1, Hnf1a), genes involved in DNA repair, differentiation and kinase activities. These genes should be viewed with caution in genomic signatures for more reliable analytical performance. Gene expression variations related to intracellular ion control and pH control processes were observed. The importance of immediate tissue cryopreservation, or at least as soon as possible after collection, was evidenced in order to minimize the effects of gene expression variation resulting from ischemia (AU)


Subject(s)
Animals , Male , Female , Cryopreservation , Gene Expression , Quality Control , Biological Specimen Banks , Microarray Analysis , Cryobiology , Ischemia/surgery
19.
Goiânia; SES-GO; 2019. 10-34 p. ilus.
Non-conventional in Portuguese | LILACS, ColecionaSUS, CONASS, SES-GO | ID: biblio-1096346

ABSTRACT

Este manual foi elaborado sob coordenação da área de Biologia Médica do Laboratório Estadual de Saúde Pública Dr. Giovanni Cysneiros (LACEN-GO), vinculado à Secretaria de Estado da Saúde, com o objetivo de orientar a coleta, o acondicionamento e o transporte de materiais para a realização de uma análise com qualidade. Ressalta-se o fato de que uma amostra coletada, armazenada ou transportada de maneira inadequada, dificilmente terá um resultado confiável, independentemente da qualidade técnica em que o ensaio for realizado. Portanto, este Manual deve ser consultado freqüentemente por todos os usuários do Sistema de Vigilância em Saúde que fazem uso dos serviços de diagnóstico laboratorial do LACEN-GO.


This manual was prepared under the coordination of the Area of Medical Biology of the State Laboratory of Public Health Dr. Giovanni Cysneiros (LACEN-GO), linked to the State Department of Health, with the objective of guiding the collection, packaging and transportation of materials to perform a quality analysis. It is noteworthy that a sample collected, stored or transported improperly will hardly have a reliable result, regardless of the technical quality in which the test is performed. Therefore, this Manual should be frequently consulted by all users of the Health Surveillance System who make use of the laboratory diagnostic services of LACEN-GO.


Subject(s)
Humans , Animals , Specimen Handling/methods , Specimen Handling/standards , Biological Specimen Banks/standards , Clinical Laboratory Techniques/methods , Clinical Laboratory Techniques/standards
20.
São Paulo; s.n; s.n; 2019. 108 p. graf, tab.
Thesis in Portuguese | LILACS | ID: biblio-1049631

ABSTRACT

Os acidentes de trânsito consistem em um grave problema de saúde pública, principalmente nos países em desenvolvimento. No Brasil, um dos recordistas mundiais nesse tipo de acidente, somente no ano de 2017 o número de mortos por essa causa foi de aproximadamente 35 mil, sendo que por volta de 12 mil eram motociclistas ou passageiros de moto. Dirigir sob efeito de substâncias psicoativas como drogas ilícitas e algumas classes de medicamentos pode aumentar significativamente o risco de ocorrências de acidentes automotivos. Pesquisas mostram que diversos fármacos psicoativos alteram a capacidade motora e cognitiva dos usuários, porém os únicos estudos brasileiros feitos com motociclistas avaliam a prevalência de uso de drogas ilícitas em usuários hospitalizados, não havendo assim trabalhos sobre o uso de outras substâncias psicoativas na população em geral de motociclistas. Visando a importância desse fato, o presente projeto avaliou a prevalência de drogas ilícitas (canabinoides, estimulantes e anfetaminas) e de fármacos psicoativos pertencentes às classes dos anti-histamínicos, relaxantes musculares, benzodiazepínicos e anorexígenos nas amostras de fluido oral de motociclistas na cidade de São Paulo. Para tal, foi desenvolvido um método analítico que utiliza a técnica de cromatografia líquida acoplada à espectrometria de massas. Além do desenvolvimento de um novo método analítico que poderá ser utilizado para o monitoramento de motoristas em geral, foram obtidos dados da prevalência do uso de drogas e medicamentos pelos motociclistas na cidade São Paulo, contribuindo assim para o desenvolvimento de medidas preventivas, políticas públicas e para o esclarecimento sobre os riscos de dirigir sob efeito de substâncias psicoativas


Traffic accidents are a serious public health problem, especially in developing countries. In Brazil, one of the world record holders in this kind of accident, in 2017 the number of death due to this cause was approximately 35 thousand, and nearly 12 thousand were motorcyclists or motorcycle passengers. Driving under the influence of psychoactive substances such as illicit drugs and some prescription drugs can significantly increase the risk of motor vehicle accidents. Researches shows that several psychoactive drugs alter the motor and cognitive capacity of users, but the few studies done in Brazil with motorcyclists evaluate the prevalence of illicit drug use in hospitalized users, thus there is no work on the use of other psychoactive substances in the general population of bikers. Considering the importance of this fact, the present project evaluated the prevalence of illicit drugs (cannabinoids, stimulants, and amphetamines) and psychoactive prescription drugs belonging to the classes of antihistamines, muscle relaxants, benzodiazepines and anorectics in motorcyclist's oral fluid samples in the city of São Paulo. Therefore, an analytical method has been developed using liquid chromatography coupled to mass spectrometry. A new analytical method was developed and validated and may be used to monitor drivers in general. Data about drugs prevalence and drug use by motorcyclists in São Paulo city were obtained contributing to the development of preventive measures, public policies and for clarification on the risks of driving under the influence of psychoactive substances


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Motorcycles/classification , Laboratory and Fieldwork Analytical Methods/analysis , Biological Specimen Banks , Substance-Related Disorders/prevention & control , Psychotropic Drugs/adverse effects , Illicit Drugs/adverse effects , Validation Study
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