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1.
Braz. oral res. (Online) ; 33: e043, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001605

ABSTRACT

Abstract: Proteomic techniques have become popular in medicine and dentistry because of their widespread use in analyzing bodily fluids such as blood, saliva, urine, and gingival crevicular fluids as well as hard tissues such as enamel, dentine, and cementum. This review is a guide to proteomic techniques in general dentistry, summarizing techniques and their clinical application in understanding and diagnosing diseases and their use in identifying biomarkers of various diseases.


Subject(s)
Humans , Saliva/chemistry , Sjogren's Syndrome/diagnosis , Proteome , Proteomics/methods , Salivary Proteins and Peptides/chemistry , Mass Spectrometry/methods , Mouth Neoplasms/diagnosis , Biomarkers/chemistry , Electrophoresis, Polyacrylamide Gel
2.
Rev. Soc. Bras. Med. Trop ; 51(5): 665-669, Sept.-Oct. 2018. graf
Article in English | LILACS | ID: biblio-1041484

ABSTRACT

Abstract INTRODUCTION: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. METHODS: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. RESULTS: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. CONCLUSIONS: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays.


Subject(s)
Humans , Urea/pharmacology , Immunoglobulin G/blood , Antibodies, Protozoan/blood , Leishmaniasis/immunology , Chagas Disease/immunology , Cross Reactions/immunology , Antibody Affinity/immunology , Urea/chemistry , Brazil/epidemiology , Enzyme-Linked Immunosorbent Assay , Biomarkers/chemistry , Leishmaniasis/complications , Leishmaniasis/diagnosis , Leishmaniasis/epidemiology , Population Surveillance , Sensitivity and Specificity , Chagas Disease/complications , Chagas Disease/diagnosis , Chagas Disease/epidemiology
3.
Braz. j. biol ; 78(1): 98-107, Feb. 2018. tab, graf
Article in English | LILACS | ID: biblio-888842

ABSTRACT

Abstract The family Verbenaceae hosts important species used in traditional medicine of many countries. The taxonomic controversies concerning the specific delimitation of several of its species make it difficult to guarantee the botanical origin of herbal preparations based on species of this family. To contribute to the development of both specific chemomarkers and a quality control tool to authenticate the botanical origin of herbal preparations of Verbenacea species, we determined the foliar HPLC-DAD phenolic profiles and the antioxidant properties of 10 wild species of this family occurring in Mexico. The contents of phenols and flavonoids varied significantly among species. Priva mexicana showed the highest levels of total phenolics (53.4 mg g-1 dry tissue) and Verbena carolina had the highest levels of flavonoids (17.89 mg g-1 dry tissue). Relevant antioxidant properties revealed by antiradical and reducing power were found for the analyzed species. These properties varied significantly in a species-dependent manner. The phenolic compounds accumulated were flavones and phenolic acids. Flavones were the only type of flavonoids found. The results of a cluster analysis showed that the compounds were accumulated in species-specific profiles. The phenolic profiles are proposed as valuable chemomarkers that can become a useful tool for the quality control concerning the botanical origin of herbal medicinal preparations based on the species analyzed. In addition, phenolic profiles could contribute importantly to solve the taxonomic controversies concerning species delimitation in the family Verbenaceae.


Resumo A família Verbenaceae compreende importantes espécies utilizadas na medicina popular de muitos países. As dificuldades taxonômicas relativas à delimitação específica de muitas das suas espécies face difícil a verificar a origem botânico das preparações herbales baseadas nas espécies desta família. Para fazer uma contribuição ao desenvolvimento de indicadores taxonômicos e dum método de controle de qualidade para verificar a origem botânico de preparações herbales das espécies de Verbenaceae, os perfis fenólicos, obtidos pares HPLC-DAD, e as atividades antioxidantes das folhas de 10 espécies selvagens Mexicanas desta família foram determinados. Os conteúdos dos compostos fenólicos totais e dos flavonoides foram significativamente diferentes entre as espécies. Priva mexicana apresentou a maior quantidade de compostos fenólicos totais (53.4 mg g-1 amostra seca) e Verbena carolina apresentou a maior quantidade de flavonoides (17.89 mg g-1 amostra seca). Verifica-se importantes propriedades antioxidantes, como os resultados dos ensaios da capacidade antiradical e do poder redutor indicaram. As propriedades antioxidantes foram significativamente diferentes entre as espécies. Verificou-se que os compostos fenólicos conteúdos nas folhas das espécies analisadas foram só flavonas e ácidos fenólicos. Os resultados das análises de agrupamento provarãn que os perfiles fenólicos foram espécie-específicos. Estes perfis podem ser considerados como indicadores químicos da qualidade relativa à origem botânico de preparações medicinais baseadas nas espécies analisadas e podem fazer importantes contribuições para a delimitação específica na família Verbenaceae.


Subject(s)
Phenols/analysis , Phenols/chemistry , Plant Extracts/analysis , Plant Extracts/chemistry , Antioxidants/analysis , Antioxidants/chemistry , Flavonoids/analysis , Flavonoids/chemistry , Biomarkers/analysis , Biomarkers/chemistry , Chromatography, High Pressure Liquid , Verbenaceae , Mexico
4.
Acta bioquím. clín. latinoam ; 51(4): 621-628, dic. 2017. ilus, graf, tab
Article in Spanish | LILACS | ID: biblio-886144

ABSTRACT

El cáncer de mama (CM) es una de las principales causas de muerte en México. Se ha observado un incremento en la incidencia de éste en mujeres de 15-29 años. A fin de comprender las causas en el desarrollo del CM, se pretendió buscar la asociación entre los genes/enfermedad empleando técnicas de Biología Molecular. Se analizaron, por genómica funcional, 50 biopsias frescas de pacientes con CM (BFCM), 50 biopsias embebidas en parafina de CM (BEPCM) y 10 biopsias frescas de pacientes con sospecha de CM (BFSC), obtenidas de mujeres que residen en Coahuila, México. Las muestras proteicas se cuantificaron y se resolvieron en geles de poliacrilamida dodecil sulfato de sodio (SDS-PAGE) y en dos dimensiones (2-DE). El perfil proteico de las BFCM, BEPCM versus BFSC mostró diferencias entre las bandas peptídicas observadas en los geles. Aquellos péptidos que se diferenciaron por su expresión fueron analizados por cromatografía líquida acoplada a masas en tándem (LC/ MS/MS). Las huellas peptídicas obtenidas, a su vez, se analizaron por medio del banco de genes (PubMed). Se encontraron, en las muestras de cáncer, proteínas asociadas a migración celular, supresión de tumores, estrés oxidativo y choque térmico. Por último, estos hallazgos se confirmaron empleando inmuno-electro transferencia o Western blot (WB) con anticuerpos contra vimentina.


Breast cancer (BC) is one of the leading causes of death in Mexico. Moreover, BC is the main cause of death in women between 15-29 years old in northern Mexico. Proteomic techniques have been used in order to achieve a better understanding of the genes involved in the development of BC. The proteins in BC extracted from 50 fresh breast cancer tissues (FBCT), 50 paraffin embedded breast cancer tissues (PEBCT) and 10 biopsies from women suspected of cancer (SC), residing in Coahuila, Mexico were analyzed in this paper. The quantity of protein extracted was similar in both samples FBCT and PEBCT. However, protein quality was lower in PEBCT than FBCT. Subsequently, these proteins were resolved in SDS-PAGE and 2DE. Differences were noticed in protein profile and all those suspect proteins were analyzed by LC/MS/MS. Amino acidic fingerprint allowed for the identification of peptides associated with a) cell migration, b) tumor suppression, c) oxidative stress or heat shock.


O câncer da mama (CM) é uma das principais causas de morte no México. Observou-se um aumento na incidência desse câncer em mulheres entre os 15-29 anos de idade. Para compreender as causas do desenvolvimento de CM, visou-se encontrar a associação entre os genes/doença utilizando técnicas de Biologia molecular. Analisaram-se por genômica funcional, 50 biópsias frescas de pacientes com CM (BFCM), 50 biópsias embebidas em parafina (BEPCM) e 10 biópsias frescas de pacientes com suspeita de CM (BFSC), obtidas de mulheres residentes em Coahuila, México. As amostras de proteínas foram quantificadas e separadas em géis de poliacrilamida dodecil sulfato de sódio (SDS-PAGE) e em duas dimensões (2-DE). O perfil proteico das BFCM, BEPCM comparado com BFSC mostrou diferenças entre as bandas peptídicas observadas nos géis. Esses peptídeos que diferem em sua expressão foram analisados por cromatografia líquida acoplada a massas em tandem (LC/MS/MS). As pegadas peptídicas obtidas, por sua vez, foram analisadas utilizando o banco de genes (PubMed). Verificaram-se nas amostras de câncer, proteínas associadas à migração celular, supressão de tumores, estresse oxidativo e choque térmico. Finalmente, estes achados foram confirmados utilizando a imuno-eletro transferência ou Western Blot (WB) com anticorpos contra vimentina.


Subject(s)
Humans , Female , Biomarkers/chemistry , Breast Neoplasms , Peptides/genetics , Chromatography, Liquid/methods , Mass Spectrometry/methods , Molecular Biology , Proteomics
5.
Medisan ; 21(8)ago. 2017. ilus, tab
Article in Spanish | LILACS | ID: biblio-894646

ABSTRACT

Se realizó una investigación descriptiva y transversal de 64 pacientes mayores de 18 años, de los cuales 31 presentaban neurosis (estrés crónico) y 33 reacción situacional (estrés agudo), quienes fueron diagnosticados y atendidos en la consulta de Psiquiatría del Hospital General Docente Dr Juan Bruno Zayas Alfonso de Santiago de Cuba, en el período de mayo a noviembre del 2013, con vistas a determinar las variables bioquímicas e inmunológicas del tejido sanguíneo. Luego de efectuados los exámenes de sangre en todos los integrantes de la serie y el procesamiento estadístico de la información mediante el cálculo porcentual, la media y la desviación estándar, se pudo concluir que desde los puntos de vista bioquímico e inmunológico, la inmunoglobulina G aumenta en pacientes con reacción situacional, mientras que el cortisol se incrementa en los que padecen neurosis


A descriptive and cross-sectional investigation of 64 patients older than 18 years, of which 31 presented neurosis (chronic stress) and 33 situational reaction (acute stress) who were diagnosed and assisted in the Psychiatry Department of Dr Juan Bruno Zayas Alfonso Teaching General Hospital in Santiago de Cuba was carried out in the period of May to November, 2013, with the objective of determining the biochemical and immunologic variables of the blood tissue. After obtaining blood tests in all the members of the series and of the statistical processing of the information by means of the percentage calculation, the mean and the standard deviation, it could be concluded that from the biochemical and immunologic points of view, immunoglobulinG increases in patients with situational reaction, while cortisol is increased in those that suffer from neurosis


Subject(s)
Humans , Male , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Epidemiologic Factors , Adjustment Disorders , Multivariate Analysis , Immunologic Factors , Neurotic Disorders , Anxiety Disorders , Stress, Physiological , Secondary Care , Biomarkers/chemistry , Cross-Sectional Studies
6.
Rev. RENE ; 17(4): 490-497, jul.-ago. 2016.
Article in English, Portuguese | LILACS, BDENF | ID: biblio-835662

ABSTRACT

Verificar a associação da predição de lesão por pressão com os níveis de albumina, hematócrito ehemoglobina. Métodos: estudo documental, desenvolvido em uma Unidade de Terapia Intensiva para Adultoscom prontuários de pacientes elegíveis (n=255). Foram extraídas variáveis de caracterização sociodemográficae clínica, desenvolvimento de lesão por pressão e região; escore da escala de Braden e resultados dosmarcadores bioquímicos. Fez-se análise estatística descritiva e inferencial, adotando-se nível de significânciade 5,0%. Resultados: houve prevalência do sexo masculino (64,7%) e de pacientes cirúrgicos (69,8%). Nãohouve associação estatística significativa entre os marcadores de hematócrito e hemoglobina com a prediçãode lesão por pressão, diferentemente dos níveis de albumina (p=0,023). Conclusão: há associação de prediçãode lesão por pressão no que se refere à albumina. O aporte proteico do paciente deve ser visto com maior rigorpela equipe de saúde.


Objective: to verify the association of pressure injury prediction with albumin, hematocrit and hemoglobin levels. Methods: documentary study, developed in an Intensive Care Unit for Adults with records of eligible patients (n=255). Sociodemographic and clinical characterizations lesion, development of pressure injury and region; a score of the Braden scale and results of biochemical markers were extracted. There was a descriptive and inferential statistical analysis, adopting a significance level of 5.0%. Results: there was a prevalence of males (64.7%) and surgical patients (69.8%). There was no significant association between hematocrit and hemoglobin markers with the pressure injury prediction, unlike albumin levels (p=0.023). Conclusion: there is an injury pressure prediction association in the albumin. The protein intake of the patient should be seen in greater detail by the health team.


Subject(s)
Humans , Male , Female , Albumins/antagonists & inhibitors , Nutrition Assessment , Biomarkers , Biomarkers/chemistry , Nursing Care , Endophenotypes/chemistry , Patient Safety , Pressure Ulcer , Medical Records Department, Hospital
7.
Article in English | WPRIM | ID: wpr-13344

ABSTRACT

Pesticide formulation includes solvents (methanol and xylene) and antifreeze (ethylene glycol) whose metabolites are anions such as formic acid, hippuric acid, and oxalate. However, the effect of the anion gap on clinical outcome in acute pesticide intoxication requires clarification. In this prospective study, we compared the anion gap and other parameters between surviving versus deceased patients with acute pesticide intoxication. The following parameters were assessed in 1,058 patients with acute pesticide intoxication: blood chemistry (blood urea nitrogen, creatinine, glucose, lactic acid, liver enzymes, albumin, globulin, and urate), urinalysis (ketone bodies), arterial blood gas analysis, electrolytes (Na+, K+, Cl- HCO3 -, Ca++), pesticide field of use, class, and ingestion amount, clinical outcome (death rate, length of hospital stay, length of intensive care unit stay, and seriousness of toxic symptoms), and the calculated anion gap. Among the 481 patients with a high anion gap, 52.2% had a blood pH in the physiologic range, 35.8% had metabolic acidosis, and 12.1% had acidemia. Age, anion gap, pesticide field of use, pesticide class, seriousness of symptoms (all P < 0.001), and time lag after ingestion (P = 0.048) were significant risk factors for death in univariate analyses. Among these, age, anion gap, and pesticide class were significant risk factors for death in a multiple logistic regression analysis (P < 0.001). In conclusions, high anion gap is a significant risk factor for death, regardless of the accompanying acid-base balance status in patients with acute pesticide intoxication.


Subject(s)
Acid-Base Equilibrium , Acidosis/etiology , Adolescent , Adult , Aged , Aged, 80 and over , Anions/chemistry , Biomarkers/chemistry , Blood Gas Analysis , Chemically-Induced Disorders/mortality , Electrolytes/analysis , Female , Humans , Intensive Care Units , Logistic Models , Male , Middle Aged , Odds Ratio , Pesticides/poisoning , Prospective Studies , Risk Factors , Survival Analysis , Urinalysis , Young Adult
8.
São Paulo; s.n; s.n; 2016. 94 p. tab, graf, ilus.
Thesis in Portuguese | LILACS | ID: biblio-846667

ABSTRACT

O óleo da amêndoa de pequi (Caryocar brasiliense Camb.) é considerado um coproduto do fruto, consumido na região do Cerrado, bioma brasileiro. Ele é fundamental para agregar valor e ampliar a utilização deste fruto regional a outros setores produtivos comerciais. Relatos na literatura apontam que o óleo possui capacidade antioxidante e efeitos benéficos sobre doenças inflamatórias, que estão associados à presença de ácidos graxos insaturados e fitoquímicos em sua composição química. Por outro lado, o tetracloreto de carbono (CCl4) é uma potente hepatotoxina, capaz de gerar radicais livres que levam ao estresse oxidativo e à inflamação. Nesse contexto, o objetivo do presente estudo foi caracterizar os óleos da amêndoa de pequi obtidos artesanalmente e por prensagem a frio e verificar o efeito de seus constituintes graxos e bioativos sobre parâmetros oxidativos e inflamatórios de ratos submetidos à toxicidade aguda induzida por tetracloreto de carbono. Inicialmente, foram investigados os parâmetros de qualidade dos óleos, bem como o perfil de ácidos graxos, teores de compostos bioativos, capacidade antioxidante e estabilidade termo-oxidativa. Os óleos da amêndoa de pequi apresentaram boa qualidade e resistência termo-oxidativa e mostraram-se ricos em ácido graxo oleico, além de possuírem compostos com propriedades antioxidantes, como fenólicos, carotenoides, tocoferóis e fitosteróis. Posteriormente, o efeito do tratamento por 22 dias com óleos da amêndoa de pequi artesanal ou prensado a frio (3 mL/kg) sobre a toxicidade aguda induzida pelo CCl4 em ratos "Wistar" machos foi avaliado. Para tal, foram determinados marcadores bioquímicos séricos, perfil lipídico, peroxidação lipídica, marcadores do sistema de defesa antioxidante e detoxificante, além de parâmetros inflamatórios do tecido hepático. De maneira geral, verificou-se que os óleos da amêndoa de pequi não minimizaram as alterações hepáticas induzidas pelo CCl4, evidenciadas pelas enzimas marcadoras do dano hepático e por parâmetros inflamatórios, no entanto os animais tratados com o óleo prensado a frio aumentaram sua capacidade antioxidante


The pequi (Caryocar brasiliense Camb.) almond oil is considered a by-product of the fruit, consumed in the Brazilian savannah region. It is essential to add value and increase the use this regional fruit to other commercial productive sectors. The pequi almond oil has been reported to possess antioxidant capacity and beneficial effect on inflammatory diseases due its chemical composition in unsaturated fatty compounds and phytochemicals. On the other hand, the carbon tetrachloride (CCl4) is a potent hepatotoxin that is capable of generating free radicals and lead to oxidative stress and inflammation. In this context, the aim of this study was to characterize the pequi almond oils obtained by handmade and cold-press process and verify the effect of their fatty acids and bioactive constituents on oxidative and inflammatory parameters in rats submitted to carbon tetrachloride-induced acute toxicity. Initially, quality parameters, chemical composition and thermo-oxidative stability of the oils were investigated. The pequi almond oils showed good quality and thermo-oxidative resistance and shown to be rich in oleic fatty acid, besides the presence of compounds with antioxidant properties, such as phenolics, carotenoids, tocopherols and phytosterols. Subsequently, the effect of the oils (3 mL/kg) treatment for 22 days on acute toxicity induced by CCl4 in male Wistar rats was evaluated. The serum biochemical markers levels, lipid profile, lipid peroxidation, markers of antioxidant defense and detoxication and inflammatory parameters in liver tissue were determined. In general, it was found that the pequi almond oil not minimized liver alterations induced by CCl4, as evidenced by the liver function enzymes and the inflammatory parameters, however in animals which received the cold pressed oil was increased antioxidant capacity


Subject(s)
Animals , Male , Rats , Toxicity Tests, Acute/prevention & control , Biomarkers/chemistry , Carbon Tetrachloride Poisoning , Oxidative Stress , Ericales/classification , Carbon Tetrachloride/administration & dosage , Chromatography, Gas/methods , Lipid Peroxidation , Oils, Volatile/adverse effects
9.
Rev. Fundac. Juan Jose Carraro ; 21(41): 24-30, 2016.
Article in Spanish | LILACS | ID: biblio-835582

ABSTRACT

El propósito de este trabajo es, en primer término, determinar cuales son los factores más importantes en el diagnóstico periodontal; su evolución en el tiempo y cuál sería el futuro en la clasificación de las enfermedades periodontales en base a los avances que se están realizando en biología molecular. Se está trabajando en la investigación de los biomarcadores salivales como predictores de la enfermedad periodontal, un rápido test de saliva podría diagnosticar dicha patología (21, 22, 27, 28, 29). Las clasificaciones van evolucionando a medida que se conoce más sobre estasenfermedades (2). Aún falta mucha investigación para poder realizar una clasificación basada en la etiología (2).La biología molecular parece ser muy prometedora, ya que podríamos ser másespecíficos en la etiología de la enfermedad y esclarecer muchas de nuestras incertidumbres (21)


The purpose of this essay is to determine which are the most importantfactors in the periodontal diagnosis, its evolution in time and also determinewhich would be the future classification of periodontal diseases based on theadvances being made in molecular biology.Even today determining the causes of periodontal disease remains a complexmatter.Work is being done today in the research of salivary biomarkers as predictorsof periodontal desease, through rapid saliva test could diagnose thisdisease.21, 22, 27, 28, 29Classifications are evolving as we know more about this disease.2There is still way more research to be made in order to be able to establisha classification based in etiology.2Molecular biology seems to be very promising in that we can be more specificin the etiology of the disease and clarify many of our uncertainties.


Subject(s)
Humans , Molecular Biology/trends , Periodontal Diseases/classification , Periodontal Diseases/diagnosis , Biomarkers/chemistry , Periodontal Diseases/etiology , Saliva/chemistry
10.
Rev. bras. cardiol. (Impr.) ; 27(2): 120-127, mar.-abr.2014. tab
Article in Portuguese | LILACS | ID: lil-719584

ABSTRACT

A avaliação da função endotelial permite identificar alterações no endotélio e pode ser avaliada por métodos bioquímicos e biofísicos. Dentre estes, destaca-se a dilatação fluxo-mediada da artéria braquial (DILA) por ser uma técnica ultrassonográfica segura e não invasiva, marcador precoce de doenças cardiovasculares, pois reflete a saúde do endotélio ainda numa fase inicial, ou seja, antes do aparecimento da placa de ateroma. Esta revisão descreve os estudos realizados no Brasil que investigaram a avaliação da função endotelial. A busca dos dados foi realizada em bases da Biblioteca Virtual em Saúde (BIREME) e SciELO. Foram incluídos estudos feitos no Brasil e publicados até agosto 2012, com avaliação da função endotelial por meio da DILA. Dos 16 artigos encontrados, 7 estudos incluíram apenas indivíduos com risco cardiovascular; 8 incluíram indivíduos com e sem risco cardiovascular e somente 1 incluiu indivíduos sem risco cardiovascular. Todos os estudos que avaliaram a função endotelial em indivíduos considerados com risco para doenças cardiovasculares encontraram valores do DILA <10 %. Os estudos sugerem que esse método é confiável na avaliação da função endotelial em indivíduos com ou sem fatores de risco cardiovascular.


Endothelial function assessments help identify alterations to the endothelium and can be evaluated by biochemical and biophysical methods. These include flow-mediated dilation (FMD) of the brachial artery by ultrasonography, a technique that is a safe and non-invasive marker of early cardiovascular disease, indicating the health of the endothelium at an early stage, before the onset of atheroma. This review describes studies conducted in Brazil investigating endothelial function assessments. A search was performed through the databases of the Virtual Health Library (BIREME) and SciELO. Papers on projects conducted in Brazil and published by August 2012 were included in this study, assessing endothelial functions by FMD. Of the sixteen papers found, seven studies included only patients at cardiovascular risk; eight included patients with and without cardiovascular risk and only one included subjects without cardiovascular risk. All the studies evaluating endothelial functions in patients rated as at-risk for cardiovascular disease found FMD values below 10%. These findings suggest that this method is trustworthy for assessing endothelial functions in patients with or without cardiovascular risk factors.


Subject(s)
Humans , Brachial Artery , Atherosclerosis/prevention & control , Databases, Bibliographic/trends , Cardiovascular Diseases/prevention & control , Cardiovascular Diseases , Endothelium/physiology , Biomarkers/chemistry , Risk Factors
11.
Indian J Biochem Biophys ; 2014 Feb; 51(1): 81-86
Article in English | IMSEAR | ID: sea-154240

ABSTRACT

Annatto tree (Bixa orellana L.) is native to Brazil and is now under cultivation in many parts of world for its reddish orange ‘annatto’ dye. There are three types of landraces in annatto and they are distinguished based on fruit shape i.e., ovate, conical and hemispherical, whose pigment yield differs. Since annatto pigment yield varies with landrace, it is necessary to characterize markers towards the identification of landraces. In this study, we characterized water soluble polysaccharides (WSP) of twigs from three landraces using size-exclusion chromatography (SEC), fourier-transform Infrared spectroscopy (FT-IR), nuclear magnetic resonance spectroscopy (NMR) and gas liquid chromatography (GLC) for their potential use as chemotaxonomic markers to distinguish the landraces. GLC analysis on WSP showed hemispherical type contained 38% rhamnose, while conical and ovate types contained 17% and 34% glucose, respectively. Thus, glucose and rhamnose content of WSP could be used to distinguish the three landraces. Further, differences in calculated molecular weight as revealed by SEC (281.8, 151.3 and 79.4 kDa for conical, hemispherical and ovate types, respectively) could also be used to distinguish the three landraces.


Subject(s)
Animals , Biomarkers/chemistry , Biomarkers/metabolism , Bixaceae/classification , Bixaceae/metabolism , Polysaccharides/chemistry , Polysaccharides/metabolism , Solubility , Water/chemistry
12.
Rev. Ateneo Argent. Odontol ; 52(1): 43-49, 2014. ilus
Article in Spanish | LILACS | ID: lil-726053

ABSTRACT

Introducción: el ameloblastoma es un tumor odontogénico benigno, localmente agresivo, que debe su origen a partir de estructuras epiteliales involucradas en la odontogénesis. El objetivo del presente trabajo es identificar, por medio de técnicas inmunohistoquímicas, aspectos de los mecanismos regulatorios de proliferación celular y la relación de los diferentes subtipos histológicos con el comportamiento biológico de estos tumores. Materiales y métodos: se seleccionaron 10 ameloblastomas multiquísticos en los cuales se realizó inmunotinción con los marcadores PCNA, Ki-67 y Ciclina D1. La interpretación de las tinciones se basó en la intensidad, localización y los subtipos celulares. La valoración utilizada para contabilizar el número de células fue baja (menos del 10 por ciento), media (hasta el 50 por ciento) y alta (más del 50 por ciento). Resultados: la tinción fue positiva en 6 casos para PCNA, en 3 para Ki-67 y en 5 para ciclina D1, en las células basales periféricas, en los patrones foliculares y plexiformes, en las del esbozo del retículo estrellado y fue negativa en los patrones quísticos y acantomatosos. Conclusión: en base a los hallazgos se puede asumir que las células basales y parabasales de los patrones foliculares y plexiformes presentan mayor actividad proliferativa que otros patrones y determinarían la evolución y tratamiento


Subject(s)
Humans , Ameloblastoma/classification , Ameloblastoma/immunology , Immunohistochemistry/methods , Biomarkers/chemistry , /immunology , Proliferating Cell Nuclear Antigen/immunology , Cyclin D1/immunology
13.
Rev. Ateneo Argent. Odontol ; 52(1): 51-54, 2014.
Article in Spanish | LILACS | ID: lil-726054

ABSTRACT

La fosfatasa alcalina (ALP) es una enzima relacionada con la enfermedad periodontal (EP). Se encuentra en los polimorfonucleares (PMN), osteoblastos, fibroblastos y diversas céluals del tejido conjuntivo. Juega un papel importante en el remodelado del tejido óseo y del ligamento periodontal. Los niveles de ALP son elevados en los sitios con pérdida de inserción, permitiendo el diangóstico de EP y la vigilancia del tratamiento periodontal


Subject(s)
Humans , Periodontal Diseases/immunology , Alkaline Phosphatase/immunology , Biomarkers/chemistry , Disease Progression , Periodontal Diseases/diagnosis , Neutrophils/physiology , Bone Remodeling/physiology
14.
Rev. Fundac. Juan Jose Carraro ; 18(37): 46-48, mar.-abr. 2013.
Article in Spanish | LILACS | ID: lil-714978

ABSTRACT

Uno de los tejidos más afectados en esta enfermedad es el tejido óseo que recubre a la pieza dentaria (hueso alveolar), que como consecuencia de la progresión de la enfermedad, se va produciendo una reabsorción y destrucción total del mismo. Sería de utilidadpoder contar con algún criterio diagnóstico, como son los marcadores biológicos, que permita detectar la posible agresión a los tejidos. La FAL ósea es una enzima directamente relacionada con el metabolismo osteológico de nuestro organismo, particularmente cuando se produce el remodelamiento (reabsorción-neoformación) del mismo. El propósito de esta revisión bibliográfica es determinar el rol de la fosfatasa alcalina salival como marcador bioquímico de la enfermedad periodontal.


Subject(s)
Humans , Periodontal Diseases/diagnosis , Alkaline Phosphatase/physiology , Saliva , Biomarkers/chemistry
15.
Biol. Res ; 46(2): 177-182, 2013. graf, tab
Article in English | LILACS | ID: lil-683995

ABSTRACT

Quillaja saponaria Mol. (Quillajaceae) is one of the most important melliferous species in Chile, mainly as a source of monofloral honey. Honey made by A. mellifera presents biological activity against pathogens and antioxidant capacity associated with the presence of phenolic compounds deriving from the nectar, as a result of bee honey foraging. The aim of this study was to identify and quantify the phenolic compounds from the floral nectar of Q. saponaria and the honey made in apiaries in the central zone, and compare the composition of the chromatographic profiles of nectar and honey to known phenolic compounds. The results obtained by HPLC-DAD (high-performance liquid chromatography with diode-array detection) showed a similar profile of phenolic compounds, in which gallic acid, myricetin, rutin, quercetin and naringenin were identified. The phenolic compounds detected could be used as a reference for future studies for determining potential chemical markers of this honey, complementing the present identification of honeys by determining their botanical origin. The identification of bioindicators of the floral origins for honey of this species could provide added value to honey commercialization by certifying the botanical origin of their chemical features and biological attributes.


Subject(s)
Honey/analysis , Phenols/analysis , Plant Extracts/chemistry , Plant Nectar/chemistry , Quillaja/chemistry , Antioxidants , Biological Factors , Biomarkers/chemistry , Chromatography, High Pressure Liquid , Gallic Acid/chemistry
16.
São Paulo; s.n; 2012. 133 p. ilus, tab, graf.
Thesis in Portuguese | LILACS | ID: lil-691543

ABSTRACT

Introdução: As doenças crônicas não transmissíveis (DCNT), como o diabetes, apresenta estreita relação com os marcadores do estresse oxidativo e da inflamação. Estes marcadores podem ser modulados pelos compostos bioativos presentes nos alimentos. Os compostos fenólicos presentes no alecrim (Rosmarinus officinalis L.) possuem atividades biológicas importantes, como antioxidante, antiinflamatória, anticarcinogênica, entre outras. Objetivo: Avaliar o efeito dos compostos fenólicos do alecrim (Rosmarinus officinalis L.) na inflamação aguda e sobre os marcadores de estresse oxidativo de ratos diabéticos induzidos por estreptozotocina. Métodos: Extrato aquoso (EA) e frações ricas em compostos fenólicos foram obtidos das folhas de alecrim e avaliados quanto à sua composição em fenólicos e capacidades antioxidante e anti-inflamatória in vitro. Ratos Wistar, machos, foram tratados com EA, fração hidroalcoólica (FHA) ou fração de ácidos fenólicos livres (AFL). O efeito do EA, FHA e AFL foram avaliados em ratos diabéticos. Foi avaliada a atividade anti-inflamatória in vivo do EA nos modelos de inflamação aguda: edema de pata e bolsa de ar. O efeito do EA também foi investigado em células de hepatócitos humano (HepG2). Para análise dos resultados utilizou-se a análise de variância (ANOVA, post-hoc Tukey), adotando como nível de significância p<0,05. Resultados: O EA e suas frações apresentaram alto conteúdo em compostos fenólicos, entre eles os flavonoides. Os ácidos rosmarínico e carnósico foram os compostos majoritários das frações de ácidos fenólicos. A composição em compostos fenólicos contribuiu significativamente para a expressiva capacidade antioxidante e inibitória da lipoxigenase in vitro. O EA de alecrim alterou positivamente a atividade das enzimas antioxidantes, reduziu as concentrações séricas de TNF-α e IL-6; e aumentou níveis de LTB4 em animais diabéticos quando comparado ao grupo controle diabético (D-H2O). A AFL não atenuou os marcadores de estresse...


ntroduction: Non-transmissible chronic diseases, such as diabetes, are strongly associated with oxidative stress and inflammation markers, which are known to be modulated by bioactive compounds found in foods. Polyphenols present in rosemary (Rosmarinus officinalis L.) display important biological activities, including among others, antioxidant, anti-inflammatory and anticarcinogenic effects. Objective: To assess the effects of polyphenols from rosemary (Rosmarinus officinalis L.) on acute inflammation and oxidative stress markers in rats with streptozotocin-induced diabetes. Methods: Aqueous extract and phenolic acid fractions were obtained from rosemary leaves and assayed for their phenolic composition and in vitro antioxidant and anti-inflammatory activities. The effects of the aqueous extract (EA), the hydroalcoholic fraction (FHA) and the free phenolic fraction (AFL) were assessed in diabetic male Wistar rats. The in vivo anti-inflammatory activity of EA was evaluated against two models of acute inflammation: paw edema and air pouch. The effect of EA was also investigated in human hepatocyte cells (HepG2). Results were compared using a one-way Analysis of Variance (ANOVA) and the Tukey post-test at a significance level of p<0.05. Results: Polyphenols, mainly flavonoids, occurred in high quantities in EA and its fractions. Rosmarinic and carnosic acids were found chiefly in the phenolic fractions. The rich phenolic composition contributed significantly to the high in vitro antioxidant activity and lipoxygenase inhibiting capacity observed. The rosemary aqueous extract (EA) positively affected antioxidant enzyme activities, reduced serum TNF-α and IL-6 concentrations and elevated LTB4 levels in diabetic rats, compared with those in the diabetic control group (D-H2O). Treatment with AFL did not attenuate the oxidative stress markers altered by diabetes. In the acute inflammation models, paw edema was reduced by EA as successfully as by indomethacin (p>0.05)...


Subject(s)
Animals , Male , Rats , Phenolic Compounds/analysis , Diabetes Mellitus , Inflammation , Biomarkers/chemistry , Oxidative Stress , Rosmarinus/adverse effects , Analysis of Variance , Diabetes Mellitus, Experimental , Inflammation/chemically induced
17.
São Paulo; s.n; 2012. 158 p.
Thesis in Portuguese | LILACS | ID: lil-666584

ABSTRACT

Introdução A obesidade está associada a um quadro de inflamação crônica e de baixa intensidade, que predispõe à resistência à ação da insulina e ao desenvolvimento do diabetes mellitus (DM) tipo 2. O aumento da concentração sanguínea de ácidos graxos não esterificados e de citocinas pró-inflamatórias eleva a expressão hepática de quinases (quinase do inibidor do fator nuclear kappa B (IKK)- e a c-jun N-terminal quinase (JNK)) que aumentam a resposta inflamatória e prejudicam a via de sinalização da insulina. A erva-mate (Ilex paraguariensis) contém compostos bioativos que apresentam a capacidade de reduzir a expressão de genes envolvidos na resposta inflamatória. Objetivo Investigar o efeito da ingestão do extrato aquoso de erva-mate (EAEM) sobre a resposta inflamatória e via de sinalização da insulina no fígado de ratos alimentados com ração hiperlipídica (HL). Métodos Ratos Wistar, machos, foram submetidos à dieta controle (CON) (n= 18) ou HL (n= 18) durante 12 semanas. Após esse período, seis animais de cada grupo foram eutanasiados, enquanto o restante dos animais foi distribuído em grupos que receberam, ou não, por gavagem, o EAEM (1 g/kg massa corporal/dia) durante quatro semanas. Após esse período, todos os animais foram eutanasiados e determinou-se as concentrações de glicose, insulina, colesterol total, HDL-colesterol, LDL-colesterol, VLDL-colesterol, triacilgliceróis (TAG), leptina, adiponectina, TNF-, IL-6, inibidor do ativador do plasminogênio-1 (PAI-1) e a MCP-1 e a atividade sérica das enzimas alanina aminotransferase (ALT) e aspartato aminotransferase (AST). Foi realizada análise histológica para verificação da infiltração de lipídios. Foi avaliada também a composição corporal dos animais. Para a análise da expressão das proteínas JNK, IKK-, AKT e IRS-1 nas suas formas totais e fosforiladas, e da proteína NF-B, na sua forma fosforilada, um grupo de animais (n = 36) foi submetido ao mesmo protocolo experimental, porém neste foi realizada a inf...


Subject(s)
Animals , Male , Rats , Diet , Dietary Fats/metabolism , Inflammation , Ilex paraguariensis/chemistry , Insulin/metabolism , Metabolism , Eating , Liver/chemistry , Biomarkers/chemistry , Obesity , Rats, Wistar
18.
Rev. colomb. biotecnol ; 13(2): 51-62, dic 1, 2011. graf
Article in Spanish | LILACS | ID: lil-645167

ABSTRACT

La papa, cultivo de importancia a nivel mundial es gravemente afectado por gota, enfermedad ocasionada por el oomycete Phytophthora infestans. Actualmente la forma más efectiva para combatir la enfermedad es mediante el desarrollo de cultivares resistentes al patógeno. Para esto, una estrategia es identificar genes que confieran resistencia al patógeno, para lo cual se buscan marcadores asociados con el carácter de resistencia. En este estudio se evaluaron marcadores moleculares tipo SCAR (Sequence Characterized Amplified Region): CosA, GP179, BA47f2 y Prp1 asociados con resistencia a P. infestans y el gen de resistencia R1, en 22 cultivares tetraploides pertenecientes a la subespecie andigena y cinco especies silvestres. Se evaluó el polimorfismo y se determinó si los alelos polimórficos permitían diferenciar genotipos resistentes de susceptibles. Se comparó el tamaño de los fragmentos obtenidos con los fragmentos esperados asociados con resistencia de acuerdo a reportes. El análisis se realizó considerando presencia/ausencia de los fragmentos: CosA210, CosA250, R11400, R11800, BA47f2500, GP179570, Prp1300, Prp1600, y Prp1900. Los resultados indicaron que en los cultivares tetraploides y silvestres, se presentaron polimorfismos en todos los marcadores evaluados, con excepción del marcador GP179. No se encontró correlación entre el rasgo de resistencia y los alelos. Los resultados de este estudio muestran que hay repuesta diferencial a los marcadores entre las subsp. tuberosum y subsp. Andigena.


Potato is an important worldwide crop seriously affected by late blight disease caused by the oomycete Phytophthora infestans. Currently, the most effective way to control the disease is developing resistant cultivars to the pathogen by identifying genes that confer resistance to the pathogen. For this purpose it is important to find molecular markers associated with the trait. In this study, the SCAR (Sequence Characterized Amplified Region) markers: CosA, GP179, BA47f2 y Prp1, associated with late blight and the resistant gen R1 were evaluated in 22 tetraploid cultivars from subspecie andigena and five wild potato species. Polymorphism was evaluated and it was evaluated if polymorphic alleles allow differentiating resistant from susceptible genotypes. The fragment length for each marker was compared to the allele size reported associated to resistance. The analysis considered the presence/absence of the fragments: CosA210, CosA250, R11400, R11800, BA47f2500, GP179570, Prp1300, Prp1600 and Prp1900. The results indicated that both, tetraploid cultivars and wild potatoes, showed polymorphisms with all these markers, except with the GP179 marker. It was not found correlation between resistance and the presence of specific alleles. Evidence found in this study indicates that results obtained with molecular markers differed between subsp. tuberosum and subsp. andigena.


Subject(s)
Biomarkers/analysis , Biomarkers/metabolism , Biomarkers/chemistry , Biomarkers, Pharmacological/analysis , Biomarkers, Pharmacological/cerebrospinal fluid , Biomarkers, Pharmacological/metabolism , Biomarkers, Pharmacological/chemistry
19.
GEN ; 64(1): 19-20, mar. 2010. tab
Article in Spanish | LILACS | ID: lil-664457

ABSTRACT

Para diseñar programas eficientes de vacunación masiva, debemos conocer la prevalencia de infección por virus de Hepatitis B y C en la población de embarazadas. Dado que la data regional es escasa, llevamos a cabo un estudio prospectivo de determinación de AgHBs, antiHBc, anti-HBs y antiVHC. Se recolectaron muestras serológicas de 3.588 embarazadas en control pre-natal referidas al Laboratorio del Sistema Regional de Salud del Estado Zulia, independientemente de edad de gestación en el período Enero 2007-Diciembre 2008. Las muestras fueron procesadas por ELISA (TecnoSuma). Los casos positivos se refirieron a Hepatología del Hospital Universitario de Maracaibo. Se detectó AgHBs(+) 3 casos (0,08%) y la combinación AgHBs(-), antiHBc(+), antiHBs(+) que indica seroconversión adecuada en 69 casos (1,92%). Cinco casos (0.13%) se reportaron AgHBs (-), antiHBc(+), antiHBs(-). La mayoría no había tenido contacto con VHB, pero 7,24% de ellas (260 casos) habían sido vacunadas y tenían marcadores negativos. La prevalencia de Hepatitis B y C fue de 2.1% y 0,44% (16 casos) respectivamente. A pesar de la baja prevalencia, madres AgHBs+ tienen gran riesgo de trasmisión vertical y sus hijos se beneficiarían del despistaje para garantizarles inmunización activa y pasiva inmediatamente después del parto...


In order to design adequate vaccination programs it is necessary to known the Hepatitis B and C virus infection prevalence among pregnant women. Given our poor regional data, we conducted a prospective study for HBsAg, anti-HBc, anti-HBs y anti-HCV seropositivity. Serological samples from 3.588 pregnant women no matter gestational age, referred to the Pre-birth Control Consult at the Regional System of Health in Zulia State, between January 2007 and December 2008. Samples were processed by ELISA (TecnoSuma). Positive cases were referred to the Hepatology consult at the Maracaibo University Hospital. HBsAg prevalence was 0.08% (3 cases). We found 1, 92% prevalence (69 cases) for HBsAg (-), antiHBc(+), AntiHBs(+), indicator of adequate seroconversion. Five cases (0, 13%) were reported as HBsAg (-), antiHBc(+), AntiHBs(-). Most women had not contact with HB virus but 260 cases (7, 24%) had received the vaccine and they had other negative markers. Hepatitis B prevalence was 2,1% and Hepatitis C prevalence was 0,44% (16 cases). Despite low prevalence, mothers HBsAg+ have great risk to infection vertical transmission to their child and they will be beneficiated from the HBV screening in order to receive immediate active and passive immunization after delivery...


Subject(s)
Humans , Adult , Female , Young Adult , Hepacivirus/isolation & purification , Biomarkers/chemistry , Communicable Disease Control , Hepatitis B virus/isolation & purification , Infectious Disease Medicine , Virology
20.
São Paulo; s.n; 2010. 98 p. ilus, tab, graf.
Thesis in Portuguese | LILACS | ID: lil-593588

ABSTRACT

A esclerose lateral amiotrófica (ELA) é uma doença neurodegenerativa que afeta os neurônios motores levando a atrofia muscular e morte por insuficiência respiratória. Esta patologia se manifesta de forma esporádica ou familiar, que são indistinguíveis clinicamente. Mutações na enzima antioxidante superóxido dismutase 1 (hSod1) respondem por aproximadamente 20% dos casos familiares de ELA. Além disso, o caráter autossômico dominante destas mutações revela que a hSod1 adquire propriedades tóxicas aos neurônios motores. Atualmente, duas hipóteses não mutuamente excludentes existem para explicar o caráter tóxico das mutantes da hSod1 relacionadas à ELA. A primeira refere-se à produção de oxidantes pela atividade peroxidásica exacerbada das mutantes contribuindo para o estresse oxidativo observado em ELA. A segunda refere-se à agregação de proteínas como ocorre em outras doenças neurodegenerativas. Digno de nota, o radical carbonato produzido na atividade peroxidásica da hSod1 causa a formação de um dímero covalente da proteína análogo a uma espécie de hSod1 frequentemente detectada em modelos experimentais e pacientes da doença e associada à propriedade tóxica das mutantes. Desta forma, o presente trabalho buscou esclarecer o mecanismo de produção do radical carbonato pela hSod1, bem como caracterizar o dímero covalente da proteína para posterior estudo de sua formação em um modelo de ELA em ratos que superexpressam a mutante G93A da hSod1. Os estudos cinéticos da variação do pH sobre os efeitos de bicarbonato/CO2, nitrito e formato na atividade peroxidásica da hSod1, medidos pelo consumo de peróxido de hidrogênio e produção de radical, permitiram excluir o mecanismo de Fenton para explicar o ciclo peroxidativo da enzima em tampão bicarbonato em favor de outros intermediários reativos. Já, os experimentos de 13C RMN, modelagem molecular e cinética de fluxo interrompido com mistura assimétrica demonstraram que o ânion peroxomonocarbonato constitui o precursor...


Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease of motors neurons that causes muscle atrophy, weakness, and death by respiratory failure. This pathology occurs in both sporadic and familiar forms that are clinically indistinguishable. Mutations in the antioxidant enzyme superoxide dismutase 1 (hSod1) respond to about 20% of the familiar cases of ALS. Besides, the autosomal dominant nature of these hSod1-associated ALS suggests that the mutants gain toxic properties to motor neurons. Currently, two hypotheses exist to explain the toxicity of hSod1 mutants but they do not exclude each other. The first one is related to the production of oxidants by the increased peroxidase activity of the ALS-linked mutants that could contribute to the oxidative stress reported in ALS. The second refers to protein aggregation as proposed in other neurodegenerative diseases. Noteworthy, the carbonate radical produced during hSod1 peroxidase activity leads to the formation of a covalent dimer of the protein similar to a hSod1 species often detected in experimental models and patients of the disease and implicated in the toxic properties of hSod1 mutants. Thus, the present work aimed to determine the mechanism of carbonate radical production by hSod1 and to characterize the covalent dimer of the protein in vitro followed by the study of covalent aggregates of hSod1 in a rat model of ALS that overexpresses the G93A mutant of the protein. The kinetic studies of the effect of bicarbonate/CO2, nitrite and formate in the peroxidase activity of hSod1 at various pH, measured by hydrogen peroxide consumption and radical production, permitted to exclude the Fenton mechanism to explain the enzyme peroxidative cycle in bicarbonate buffer in favor of other reactive intermediates. Furthermore, 13C NMR, molecular docking and stopped-flow experiments with asymmetric mixing demonstrated that the anion peroxomonocarbonate is the precursor of the carbonate radical produced by...


Subject(s)
Dimerization , Enzyme Activation , Enzymes , Amyotrophic Lateral Sclerosis/physiopathology , Peroxidase , Superoxide Dismutase/chemistry , Free Radicals , Biomarkers/analysis , Biomarkers/chemistry , Neurodegenerative Diseases
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