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1.
Vitae (Medellín) ; 28(3)2021-08-11. Ilustraciones
Article in English | LILACS, COLNAL | ID: biblio-1363266

ABSTRACT

BACKGROUND: Biosensing techniques have been the subject of exponentially increasing interest due to their performance advantages such as high selectivity and sensitivity, easy operation, low cost, short analysis time, simple sample preparation, and real-time detection. Biosensors have been developed by integrating the unique specificity of biological reactions and the high sensitivity of physical sensors. Therefore, there has been a broad scope of applications for biosensing techniques, and nowadays, they are ubiquitous in different areas of environmental, healthcare, and food safety. Biosensors have been used for environmental studies, detecting and quantifying pollutants in water, air, and soil. Biosensors also showed great potential for developing analytical tools with countless applications in diagnosing, preventing, and treating diseases, mainly by detecting biomarkers. Biosensors as a medical device can identify nucleic acids, proteins, peptides, metabolites, etc.; these analytes may be biomarkers associated with the disease status. Bacterial food contamination is considered a worldwide public health issue; biosensor-based analytical techniques can identify the presence or absence of pathogenic agents in food. OBJECTIVES: The present review aims to establish state-of-the-art, comprising the recent advances in the use of nucleic acid-based biosensors and their novel application for the detection of nucleic acids. Emphasis will be given to the performance characteristics, advantages, and challenges. Additionally, food safety applications of nucleic acid-based biosensors will be discussed. METHODS: Recent research articles related to nucleic acid-based biosensors, biosensors for detecting nucleic acids, biosensors and food safety, and biosensors in environmental monitoring were reviewed. Also, biosensing platforms associated with the clinical diagnosis and food industry were included. RESULTS: It is possible to appreciate that multiple applications of nucleic acid-based biosensors have been reported in the diagnosis, prevention, and treatment of diseases, as well as to identify foodborne pathogenic bacteria. The use of PNA and aptamers opens the possibility of developing new biometric tools with better analytical properties. CONCLUSIONS: Biosensors could be considered the most important tool for preventing, treating, and monitoring diseases that significantly impact human health. The aptamers have advantages as biorecognition elements due to the structural conformation, hybridization capacity, robustness, stability, and lower costs. It is necessary to implement biosensors in situ to identify analytes with high selectivity and lower detection limits


ANTECEDENTES: Las técnicas de biodetección han sido objeto de un interés cada vez mayor debido a ventajas, tales como alta selectividad y sensibilidad, facilidad de manejo, bajo costo, tiempo de análisis corto, preparación sencilla de muestras y detección en tiempo real. Los biosensores se han desarrollado integrando la especificidad única de las reacciones biológicas y la alta sensibilidad de los sensores físicos. Por lo tanto, las técnicas de biodetección han tenido un amplio campo de aplicación y hoy en día son omnipresentes en diferentes áreas del medio ambiente, la salud y la seguridad alimentaria. Se han utilizado biosensores para estudios ambientales, detectando y cuantificando contaminantes en el agua, el aire y el suelo. Los biosensores también mostraron un gran potencial para desarrollar herramientas analíticas con innumerables aplicaciones en el diagnóstico, prevención y tratamiento de enfermedades, principalmente mediante la detección de biomarcadores. Los biosensores como dispositivo médico pueden utilizarse para identificar ácidos nucleicos, proteínas, péptidos, metabolitos, etc. Estos analitos pueden ser biomarcadores asociados al estado de la enfermedad. La contaminación bacteriana de los alimentos se considera un problema de salud pública mundial; se pueden utilizar técnicas analíticas basadas en biosensores para determinar la presencia o ausencia de agentes patógenos en los alimentos. OBJETIVOS: La presente revisión tiene por objeto establecer los últimos adelantos en la utilización de biosensores basados en ácidos nucleicos y su novedosa aplicación para la detección de ácidos nucleicos. Se hará hincapié en las características del desempeño, las ventajas y los desafíos. Además, se examinarán las aplicaciones de los biosensores basados en ácidos nucleicos para la inocuidad de los alimentos. MÉTODOS: Se examinaron artículos de investigación recientes relacionados con los biosensores a base de ácidos nucleicos, los biosensores para la detección de ácidos nucleicos, los biosensores y la inocuidad de los alimentos, y los biosensores para la vigilancia del medio ambiente. También se incluyeron plataformas de biosensores asociadas al diagnóstico clínico y a la industria alimentaria. RESULTADOS: Es posible apreciar que se han reportado múltiples aplicaciones de biosensores basados en ácido nucleico para el diagnóstico, prevención y tratamiento de enfermedades, así como para identificar bacterias patógenas transmitidas por los alimentos. El uso de PNA y aptámeros abre la posibilidad de desarrollar nuevas herramientas biométricas con mejores propiedades analíticas. CONCLUSIONES: Los biosensores pueden ser considerados como los instrumentos más importantes para la prevención, el tratamiento y la vigilancia de las enfermedades que tienen un impacto significativo en la salud humana. Los aptámeros tienen ventajas como elemento de biorreconocimiento debido a la conformación estructural, capacidad de hibridación, robustez, estabilidad y menores costos. Es necesario implementar biosensores in situ para identificar analitos con alta selectividad y menores límites de detección


Subject(s)
Humans , Biosensing Techniques , Nucleic Acids , Biomarkers , Diagnosis , Noxae
2.
Chinese Journal of Biotechnology ; (12): 3890-3904, 2021.
Article in Chinese | WPRIM | ID: wpr-921474

ABSTRACT

Clustered regularly interspaced short palindromic repeats/clustered regularly interspaced short palindromic repeats -associated protein (CRISPR/Cas) has been developed as a precise, efficient, affordable and sensitive nucleic acid detection tool due to its efficient targeted binding ability and programmability. At present, biosensors based on CRISPR-Cas system have shown excellent performance in the detection of nucleic acid of pathogens, which has attracted widespread attention, and is expected to replace the conventional detection methods. This review summarizes the latest research progress of biosensors based on CRISPR/Cas system for detecting nucleic acid of pathogens.


Subject(s)
Biosensing Techniques , CRISPR-Cas Systems/genetics , Nucleic Acids/genetics
3.
Chinese Journal of Biotechnology ; (12): 911-922, 2021.
Article in Chinese | WPRIM | ID: wpr-878603

ABSTRACT

Transcription factor-based biosensors (TFBs) play an essential role in metabolic engineering and synthetic biology. TFBs sense the metabolite concentration signals and convert them into specific signal output. They hold high sensitivity, strong specificity, brief analysis speed, and are widely used in response to target metabolites. Here we reviewe the principles of TFBs, the application examples, and challenges faced in recent years in microbial cells, including detecting target metabolite concentrations, high-throughput screening, adaptive laboratory evolutionary selection, and dynamic control. Simultaneously, to overcome the challenges in the application, we also focus on reviewing the performance tuning strategies of TFBs, mainly including traditional and computer-aided tuning strategies. We also discuss the opportunities and challenges that TFBs may face in practical applications, and propose the future research trend.


Subject(s)
Biosensing Techniques , Gene Expression Regulation , Metabolic Engineering , Synthetic Biology , Transcription Factors/metabolism
4.
Psicol. Estud. (Online) ; 26: e46410, 2021. tab, graf
Article in Portuguese | LILACS, INDEXPSI | ID: biblio-1287637

ABSTRACT

RESUMO A associação entre mindfulness (MF) e Realidade Virtual (RV) é recente. O MF vem sendo utilizado na psicoterapia em razão dos efeitos terapêuticos observados. Entretanto, não são todos os pacientes que conseguem obter o benefício desse recurso, sendo a RV uma via para o trabalho com o MF por facilitar o sentido de presença. Este artigo teve como objetivo realizar uma revisão narrativa da literatura sobre a utilização terapêutica da RV na prática de MF. Foram analisados 23 artigos completos, sendo os principais focos de pesquisa: o aprimoramento dos ecossistemas imersivos e dos recursos terapêuticos no contexto do MF em relação à experiência do usuário; o desenvolvimento de recursos tecnológicos econômicos; material de fácil manuseio para facilitar a experiência do usuário. Concluímos que a RV pode ser facilitadora como ferramenta no tratamento de pacientes cujo perfil se enquadra em terapia mediada por MF, embora sejam necessários estudos controlados que permitam compreender as especificidades da RV e as variáveis com valor terapêutico.


RESUMEN La asociación entre mindfulness (MF) y Realidad Virtual (VR) es reciente. MF se ha utilizado en psicoterapia debido a los efectos terapéuticos observados. Sin embargo, no todos los pacientes pueden obtener el beneficio de este recurso, siendo la RV una forma de trabajar con el MF porque facilita el sentido de presencia. Este artículo tuvo como objetivo realizar una revisión narrativa de la literatura sobre el uso terapéutico de la RV en la práctica de MF. Se analizaron 23 artículos completos, siendo los principales focos de investigación: la mejora de ecosistemas inmersivos y recursos terapéuticos en el contexto de MF en relación a la experiencia del usuario; el desarrollo de recursos tecnológicos económicos; material fácil de manejar para facilitar la experiencia del usuario. Concluimos que la RV puede ser un facilitador como herramienta en el tratamiento de pacientes cuyo perfil encaja en la terapia mediada por MF, aunque son necesarios estudios controlados para entender las especificidades de la RV y las variables con valor terapéutico.


ABSTRACT The association between mindfulness (MF) and Virtual Reality (VR) is recent. MF has been used in psychotherapy due to the therapeutic effects reported. However, not all patients are able to obtain the benefits of this resource, and VR becomes a way to work with MF as it facilitates the sense of presence. This paper presents a narrative review of the literature about therapeutic uses of VR in the practice of MF. A total of 23 complete papers were analyzed, being the main research focuses: the improvement of immersive ecosystems and therapeutic resources in the context of MF in relation to the user experience; the development of economic technological resources; easy to handle material to facilitate the user experience. We concluded that VR can be a facilitator as a tool in the treatment of patients who could benefit from MF-informed therapy, although controlled studies are needed to understand the specificities of VR and the variables with therapeutic value.


Subject(s)
Psychotherapy , Mindfulness , Virtual Reality , Anxiety/psychology , Psychology, Clinical/trends , Therapeutics/psychology , Biosensing Techniques/trends , Meditation/psychology , Depression/psychology , Psychological Distress
5.
Braz. arch. biol. technol ; 64: e21210030, 2021. graf
Article in English | LILACS | ID: biblio-1339317

ABSTRACT

Abstract Nowadays, a prompt and reliable diagnosis is one of the most critical measures for leprosy control. The current diagnostic is based on clinical exams by a health care professional, and it may not recognize early signs of the disease. Therefore, other leprosy diagnosis methods are needed that are sensitive, disease-specific, and easy to deliver to the end-user. This study describes the construction of an electrochemical DNA biosensor to detect PCR products of Mycobacterium leprae using methylene blue as an indicator of the hybridization. The capture probe was immobilized on the graphite electrode modified with poly(4-aminophenol). The electrode surface was morphologically characterized by atomic force microscopy. Linear voltammetry was used to monitor the concentration of methylene blue on the DNA biosensor, which indicated a limit detection of 1 x 10-10 mol/L. The biosensor showed selective when placed to hybridize with a non-complementary sequence. This study suggests that the electrochemical DNA biosensor developed is promising for detecting DNA of Mycobacterium leprae.


Subject(s)
Biosensing Techniques , Aminophenols , Methylene Blue , Mycobacterium leprae
7.
Chinese Journal of Biotechnology ; (12): 1051-1059, 2020.
Article in Chinese | WPRIM | ID: wpr-826871

ABSTRACT

Neurotransmitters play an important role in nervous system. Temporal and spatial changes of neurotransmitter distribution are crucial to information processing in neural networks. Biosensors that can visually monitor neurotransmitters are one of the vital tools to explore a variety of physiological and pathological activities. This article reviews recent advances in monitoring neurotransmitters with high temporal and spatial resolution, and introduces the latest fluorescent imaging methods for typical neurotransmitters, including glutamate, dopamine, γ-aminobutyric acid and acetylcholine. The article also summarizes the basic principles, advantages and disadvantages of various visually detection methods, and provides systematic suggestions for designing neurotransmitter sensors with high temporal and spatial resolution.


Subject(s)
Animals , Biosensing Techniques , Fluorescence , Humans , Neurotransmitter Agents , Metabolism
8.
Chinese Journal of Biotechnology ; (12): 1060-1068, 2020.
Article in Chinese | WPRIM | ID: wpr-826870

ABSTRACT

Fluorescent proteins can be used as probes to investigate intercellular molecular interactions and trace the pathway of specific metabolites, thus providing a detailed and accurate description of various metabolic processes and cellular pathways in living cells. Nowadays, the existing fluorescent proteins cover almost all spectral bands from ultraviolet to far-red. These fluorescent proteins have been applied in many fields of bioscience with the help of high-resolution microscopy, making great contributions to the development of biology. It is generally agreed that orange fluorescent proteins refer to the fluorescent proteins at the spectral range of 540-570 nm. In recent years, researches on orange fluorescent proteins have made great progress, and they have been widely applied in the field of biology and medicine as reporter protein and fluorescence resonance energy transfer as fluorescent receptor. This paper reviews the studies in the field of orange fluorescent proteins over the last 15 years, with the special focus on the development and application of orange fluorescent proteins to provide the basis for the future studies.


Subject(s)
Biosensing Techniques , Fluorescence Resonance Energy Transfer , Luminescent Proteins , Metabolism , Research
9.
Article in Chinese | WPRIM | ID: wpr-878790

ABSTRACT

A highly specific electrochemical biosensor based on T-Hg~(2+)-T structure for fast screening trace Hg~(2+) in complex animal drug matrix was constructed by cyclic voltammetry(CV) and differential pulse voltammetry(DPV). In the presence of Hg~(2+), it can be specifically binded to the T base of DNA sequence on the surface of modified gold electrode, which changes the conformation of DNA molecule and the electrochemical signal. The concentration ratio of EDC/NHS, the concentration ratio of FC-DNA and the reaction time of the biosensor were optimized by the index of sensitivity and reproducibility in CV. The results showed that the stability of the biosensor was good within 3 days(RSD≤1.3%), the difference between batches was low(RSD=4.7%), and the specificity of the biosensor was high in the presence of interfering ions(As~(3+), Cd~(2+), Cu~(2+), Pb~(2+), Zn~(2+) and Fe~(3+)). DPV results showed that the peak current signal value has a linear relationship with the lgC_((Hg)) over a concentration range from 0.1 nmol·L~(-1) to 1.0 μmol·L~(-1) with a detection limit of 0.066 nmol·L~(-1). Finally, the recovery rate tested in the matrix of animal medicine was satisfactory as 99.17%-101.3%, which can meet the needs of the determination of trace Hg~(2+) in the matrix of Bombyx Batryticatus, and provide a new idea for the rapid screening of trace heavy metals in the matrix of other types of complex traditional Chinese medicine.


Subject(s)
Animals , Biosensing Techniques , DNA/genetics , Electrochemical Techniques , Gold , Mercury , Reproducibility of Results
10.
Chinese Journal of Biotechnology ; (12): 2779-2790, 2020.
Article in Chinese | WPRIM | ID: wpr-878529

ABSTRACT

Bile acids facilitate the absorption of lipids, and affect the development of various diseases by regulating intestinal flora structure and modulating immunity and metabolism. It is therefore important to quantitatively detect bile acids. Current analytical methods are still immature due to constituent complexity, structural heterogeneity and bioactive variability of bile acids. Detection of individual bile acids is of significance for pharmacological research, clinical diagnosis and disease prevention. Advances have been made in bile acid analysis from multiple sources including serum, bile, urine and feces, although several limitations still exist for bile acid quantification. Here we review research progress in conventional bile acid assays, including spectrophotometry, thin-layer chromatography, liquid/gas chromatography and liquid/gas chromatography-mass spectrometry. Moreover, we emphasize the development of bile acid biosensors that may have promising prospects.


Subject(s)
Bile , Bile Acids and Salts , Biosensing Techniques , Chromatography, Thin Layer , Gas Chromatography-Mass Spectrometry
11.
Article in Chinese | WPRIM | ID: wpr-880404

ABSTRACT

Breast cancer is one of the most serious diseases threatening women's life and health in the world, and the mortality rate is the second in the world. With the progress of nanotechnology and the advantages of nanomaterials in the field of electrochemistry and biosensor, various nanomaterials have been applied in electrochemical biosensors. This makes the electrochemical nano-biosensor in the field of rapid detection of breast cancer has been widely concerned and studied. This paper introduces the important components of electrochemical nano-biosensor for breast cancer detection and the research progress of each component in breast cancer detection, as well as the performance of electrochemical nano biosensor in breast cancer detection and the prospect of its application.


Subject(s)
Biosensing Techniques , Breast Neoplasms/diagnosis , Electrochemical Techniques , Female , Humans , Nanostructures , Nanotechnology
12.
Chonnam Medical Journal ; : 86-98, 2019.
Article in English | WPRIM | ID: wpr-763278

ABSTRACT

Biosensors are analytical devices for biomolecule detection that compromise three essential components: recognition moiety, transducer, and signal processor. The sensor converts biomolecule recognition to detectable signals, which has been applied in diverse fields such as clinical monitoring, in vitro diagnostics, food industry etc. Based on signal transduction mechanisms, biosensors can be categorized into three major types: optical biosensors, electrochemical biosensors, and mass-based biosensors. Recently, the need for faster, more sensitive detection of biomolecules has compeled researchers to develop various sensing techniques. In this review, the basic structure and sensing principles of biosensors are introduced. Additionally, the review discusses multiple recent works about nucleic acid and exosome sensing.


Subject(s)
Biosensing Techniques , Exosomes , Food Industry , In Vitro Techniques , Nucleic Acids , Signal Transduction , Transducers
14.
Chinese Journal of Biotechnology ; (12): 2269-2283, 2019.
Article in Chinese | WPRIM | ID: wpr-781639

ABSTRACT

Cell-free synthetic biology system can perform biological transcription and translation process in vitro. Because of its advanced features, such as flexible openness, easy control, short expression time and high tolerance to cytotoxicity, this systemhas been successfully used to synthesize proteins that are difficult to express in cells. With the continuous development of cell-free biosensing technology and the lyophilization technology, its applications have widely expanded into many biomedical fields. This review discusses the current research progress of cell-free synthetic biology system in on-demand biopharmaceutical synthesis, portable diagnostics, and others. Further development of the system can lead to even more complicated synthesis of therapeutic proteins with post-translational modifications and evolution of different cell-free biosensors with high sensitivity. Cell-free synthetic biology as an emerging engineering strategy can be a better means applied to high-throughput screening of pharmaceutical proteins, detection of new pathogens, and other important health-care fields in the future.


Subject(s)
Biosensing Techniques , Cell-Free System , Industry , Synthetic Biology
15.
Chinese Journal of Biotechnology ; (12): 1581-1589, 2019.
Article in Chinese | WPRIM | ID: wpr-771771

ABSTRACT

As the main factor leading to foodborne illnesses, foodborne pathogens have been attached great importance by people. The development of simple, rapid, high-sensitivity and low-cost food-borne pathogen detection methods is of great significance in reducing the incidence of foodborne diseases. Biosensor technology is a new micro-analysis technology developed by multi-disciplinary cross-infiltration. It has the characteristics of high sensitivity and fast analysis speed, and is widely used in the detection of food-borne pathogens. This paper introduces the basic principles of biosensors, summarizes the application of common biosensors in the detection of foodborne pathogens, and prospects for future development.


Subject(s)
Biosensing Techniques , Food Microbiology , Foodborne Diseases , Humans
16.
Int. j. morphol ; 36(4): 1490-1499, Dec. 2018. tab, graf
Article in Spanish | LILACS | ID: biblio-975727

ABSTRACT

Nanotecnología es la ciencia que involucra la síntesis de materiales en escala entre 1-100 nm (nanomateriales) es aplicable en diferentes áreas tales como medio ambiente, electrónica, alimentos, energía, entre otros. Los campos que serán relevantes dentro de esta revisión y explicados en detalle son la nanomedicina y la nano-odontología. Actualmente, en estas áreas los tres principales temas en desarrollo son específicamente en el sub-área de la nanobiotecnología y corresponden a: sensorización (biosensores/biodetección), diagnóstico (biomarcadores/bioimagen) y transportes de genes, proteínas o fármacos (sistemas de intercambio controlado en blancos sistémicos versus localizados). También se han presentado avances en bioaplicaciones como modelamientos de membranas, marcaje celular, entrega de agentes a blancos específicos, estrategias para prevención de enfermedades, ingeniería de tejidos, regeneración de órganos, estrategias de inmunoensayos y nano-oncología. Este artículo de revisión pretende abordar algunos de los aportes más relevantes, que tienen algunos de los trabajos recientes, sobre los sistemas de nanopartículas, principalmente aquellos dirigidos a terapias en áreas como diabetes, nano-oncología, terapia de fármacos y genes, mediante la técnica layer-by-layer y autoensamblado, muy utilizados también en ingeniería de tejidos y regeneración tisular, junto a un breve resumen de los avances que existen en el campo de la nano-odontología.


Nanotechnology is the science that involves the synthesis of materials in scale between 1-100 nm (nanomaterials) and is applicable in different areas such as environment, electronics, food, energy, among others. The fields that will be relevant within this review and explained in detail are nanomedicine and nano-dentistry. Currently, in these areas, the three main topics under development are specifically in the sub-area of nanobiotechnology and correspond to: sensorization (biosensors / biosensing), diagnostics (biomarkers / bioimaging) and transport of genes, proteins or drugs (exchange systems) controlled in systemic versus localized targets). Advances have also been presented in bioapplications such as membrane modeling, cell marking, delivery of agents to specific targets, strategies for disease prevention, tissue engineering, organ regeneration, immunoassay strategies and nano-oncology. This review article aims to address some of the most relevant contributions, some of the recent work, on nanoparticle systems, mainly those aimed at therapies in areas such as diabetes, nanooncology, drug and gene therapy, through the layer-by-layer and self-assembled technique, also widely used in tissue engineering and tissue regeneration, together with a brief summary of the advances that exist in the field of nano-dentistry.


Subject(s)
Nanomedicine/trends , Polymers/chemistry , Bone Regeneration , Biosensing Techniques , Genetic Therapy , Drug Delivery Systems , Tissue Engineering , Nanotechnology , Dentistry/trends , Quantum Dots , Medical Oncology/trends
17.
Vaccimonitor (La Habana, Print) ; 27(1)ene.-abr. 2018. ilus, tab
Article in Spanish | LILACS, CUMED | ID: biblio-1094601

ABSTRACT

Los biosensores son dispositivos móviles que permiten detectar de forma rápida y sencilla enfermedades del metabolismo e infecciones víricas de interés veterinario y clínico, como el rotavirus y la hepatitis B y C. Este trabajo tuvo como objetivo determinar las variables significativas del proceso de producción de los biosensores de glucosa fabricados en el Centro de Inmunoensayo (La Habana, Cuba). Se produjeron ocho corridas experimentales teniendo en cuenta los procedimientos normativos de operación implementados en la planta de producción de biosensores y se realizaron las evaluaciones de calidad correspondientes (pruebas de exactitud) para liberar analíticamente los lotes producidos. Los experimentos realizados proporcionaron información acerca de cuáles variables deben controlarse con más cuidado durante la producción a fin de evitar altos niveles de productos no conformes o el comportamiento errático del proceso. Las variables seleccionadas para el estudio fueron las relacionadas con la preparación de la solución enzimática. Con los resultados obtenidos se realizó un análisis de regresión múltiple para determinar los factores estadísticamente significativos del modelo, obteniéndose un coeficiente de determinación superior al 90 por ciento, logrando explicar el 98,637 por ciento de la variación entre los valores de porcentaje de exactitud y la media. Los factores que resultaron ser significativos fueron la concentración de la enzima glucosa oxidasa, la concentración del mediador eléctrico y la conductividad del agua ultrapura para un nivel de confianza del 95 por ciento. El análisis realizado arrojó resultados satisfactorios demostrando que variando parámetros del proceso productivo es posible disminuir los valores del porcentaje de exactitud(AU)


Biosensors are mobile devices that allow rapid and easy detection of metabolic diseases and viral infections of veterinary and clinical interest, such as rotavirus and hepatitis B and C. The objective of this work was to determine the significant variables of the production process of the glucose biosensors manufactured in the Immunoassay Center (Havana, Cuba). Eight experimental runs were carried out taking into account the normative operating procedures of the biosensor production plant. Accuracy tests were carried out to release produced batches. The experiments provided information about which factors should be carefully controlled during the manufacture procedure in order to avoid high levels of faulty products or the erratic behavior of the process. The factors selected for the study were those related with the preparation of the enzymatic solution. A multiple regression analysis was carried out to determine the statistically significant factors of the model. The coefficient of determination was higher than 90 percent, and the 98.637 percent of the variation between the values of percentage of accuracy and the mean value could be explained. The significant factors were the concentration of the glucose oxidase enzyme, the electric mediator concentration, and the ultrapure water conductivity (95 percent confidence level). The analysis carried out showed satisfactory results. In the present study, it was demonstrated that varying parameters of the production process it is possible to decrease the accuracy percentage values(AU)


Subject(s)
Biosensing Techniques/instrumentation , Glucose Oxidase/blood , Cuba
18.
An. acad. bras. ciênc ; 90(1,supl.1): 825-857, 2018. tab, graf
Article in English | LILACS | ID: biblio-886938

ABSTRACT

ABSTRACT Bioelectrochemistry can be defined as a branch of Chemical Science concerned with electron-proton transfer and transport involving biomolecules, as well as electrode reactions of redox enzymes. The bioelectrochemical reactions and system have direct impact in biotechnological development, in medical devices designing, in the behavior of DNA-protein complexes, in green-energy and bioenergy concepts, and make it possible an understanding of metabolism of all living organisms (e.g. humans) where biomolecules are integral to health and proper functioning. In the last years, many researchers have dedicated itself to study different redox enzymes by using electrochemistry, aiming to understand their mechanisms and to develop promising bioanodes and biocathodes for biofuel cells as well as to develop biosensors and implantable bioelectronics devices. Inside this scope, this review try to introduce and contemplate some relevant topics for enzyme bioelectrochemistry, such as the immobilization of the enzymes at electrode surfaces, the electron transfer, the bioelectrocatalysis, and new techniques conjugated with electrochemistry vising understand the kinetics and thermodynamics of redox proteins. Furthermore, examples of recent approaches in designing biosensors and biofuel developed are presented.


Subject(s)
Bioelectric Energy Sources , Biosensing Techniques , Electrochemistry , Electron Transport , Enzymes/chemistry , Enzymes/physiology
19.
An. acad. bras. ciênc ; 90(1,supl.1): 943-992, 2018. tab, graf
Article in English | LILACS | ID: biblio-886937

ABSTRACT

ABSTRACT Several enzymatic reactions of heteroatom-containing compounds have been explored as unnatural substrates. Considerable advances related to the search for efficient enzymatic systems able to support a broader substrate scope with high catalytic performance are described in the literature. These reports include mainly native and mutated enzymes and whole cells biocatalysis. Herein, we describe the historical background along with the progress of biocatalyzed reactions involving the heteroatom(S, Se, B, P and Si) from hetero-organic substrates.


Subject(s)
Bacteria/metabolism , Biotransformation , Enzymes/metabolism , Biocatalysis , Fungi/metabolism , Substrate Specificity , Biosensing Techniques , Enzymes/chemistry
20.
Article in English | WPRIM | ID: wpr-772818

ABSTRACT

Synthetic biology aims to endow living cells with new functions by incorporating functional gene networks into them. By overexpressing, blocking and rewiring native gene pathways, synthetic biologists have harnessed this promising technology to reprogram cells to perform diverse tasks such as drug discovery, biopharmaceutical manufacturing, gene therapy and tissue engineering, etc. In this review, we focus on current technologies of synthetic biosensors for disease detection. We start with the design principle of synthetic biosensors. Then we move towards the characteristics of simple synthetic biosensors, which can respond to a single input signal, and complex synthetic biosensors including Boolean gate biosensors, cascade biosensors, time-delay biosensors, oscillator biosensors and hysteretic biosensors, which can respond to more than two input signals and perform complex tasks. Synthetic biosensor has showed great potential in disease detection, but it is still in its infancy stage. More efforts should be made in identifying and constructing clinically relevant regulation systems. Computational tools are also needed in the design process in order to guarantee the precision of the synthetic biosensor. The ultimate goal of a synthetic biosensor is to act as a therapeutic sensor-effector device that connects diagnostic input with therapeutic output and therefore provides all-in-one diagnostic and therapeutic solutions for future gene- and cell-based therapies.


Subject(s)
Biosensing Techniques , Methods , Humans , Synthetic Biology , Methods
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