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1.
Braz. J. Pharm. Sci. (Online) ; 58: e181053, 2022. tab
Article in English | LILACS | ID: biblio-1360163

ABSTRACT

Abstract The effect of hypothermia treatment on white blood cell (WBC), neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR) and platelet-to-lymphocyte ratio (PLR) values as an indicator of inflammation was evaluated in newborns with hypoxic ischemic encephalopathy (HIE). The study was performed that the before-therapeutic hypothermia (TH) and after-TH WBC, lymphocytes, neutrophils, monocytes and NLR, LMR and PLR values of the complete blood cell count were retrospectively evaluated. The results of the patient group were compared with the results of healthy newborns. A total of 78 patients who underwent TH were evaluated in our study. Mean values before and after TH were NLR3.8/2.7, LMR 5.6/8.6, and PLR 60.3/67.1 respectively. A statistical significance was present for NLR values before and after TH in those with seizure in our study (4.15±2.95/3.01±2.54) but no statistical significance was found for LMR or PLR. In neonates with HIE, effect of TH on complete blood cell count and inflammatory mechanisms (mediated neutrophil and lymphocyte) may be minimal.


Subject(s)
Humans , Male , Female , Infant, Newborn , Infant, Newborn/physiology , Hypoxia-Ischemia, Brain/pathology , Hypothermia/pathology , Blood Cell Count/methods , Hypothermia/classification , Inflammation
2.
Rev. bras. neurol ; 57(3): 5-10, jul.-set. 2021. tab
Article in Portuguese | LILACS | ID: biblio-1342495

ABSTRACT

INTRODUÇÃO: À medida que a população envelhece e a expectativa de vida aumenta, a incidência global e a prevalência de AVC isquêmico tendem a aumentar significativamente. Nesse contexto, surge a necessidade de avaliar novos marcadores preditores de mortalidade, como a contagem absoluta de monócitos, relação linfócitos sobre monócitos, relação neutrófilos sobre linfócitos e níveis de proteína C reativa ultrassensível, que além de serem de fácil acesso e baixo custo, sugerem indicar desfecho no paciente com AVC agudo. OBJETIVOS: o objetivo deste estudo foi avaliar a associação dos marcadores inflamatórios com a mortalidade de pacientes com AVC isquêmico. MÉTODOS: trata-se de um estudo retrospectivo observacional a partir de prontuários eletrônicos e exames laboratoriais de pacientes com AVC isquêmico em uma unidade hospitalar de Cascavel/PR. Uma análise estatística descritiva foi conduzida para determinar o perfil dos pacientes segundo o desfecho e aplicado um modelo de regressão logística para verificar as variáveis associadas a mortalidade. Foram considerados significativos apenas os dados com p-valor <0,05. RESULTADOS: Dos 65 pacientes que foram admitidos no estudo, 50 receberam alta hospitalar e 15 foram a óbito no hospital. Entre os marcadores inflamatórios, a relação de neutrófilos sobre linfócitos (OR 1,55; p-valor <0,01) mostrou-se significativamente associada a maior chance de óbito. Os pacientes que faleceram apresentaram níveis superiores de PCR ultrassensível, maior contagem absoluta de monócitos, relação linfócitos sobre monócitos diminuída, e relação neutrófilos sobre linfócitos elevada. CONCLUSÃO: a relação de neutrófilos sobre linfócitos elevada pode estar significativamente associada ao desfecho desfavorável após um AVC isquêmico


IINTRODUCTION: As the population ages and life expectancy increases, the global incidence and prevalence of ischemic stroke tends to rise significantly. In this context, the need arises to evaluate new predictive markers of mortality, such as absolute monocyte count, lymphocyte-to-monocyte ratio, neutrophil-to-lymphocyte ratio and C-reactive protein (CRP) levels which, besides being easily accessible and affordable, manage to predict the outcome in patients with acute stroke. OBJECTIVES: the aim of this study was to evaluate the association between inflammatory markers and the mortality in ischemic stroke patients. METHODS: this is a retrospective observational study based on the analysis of electronic medical records and laboratory tests of in-patients who suffered an ischemic stroke in Cascavel/PR. A descriptive statistical analysis was conducted to determine patients´ profile according to the outcome and a logistic regression model was applied in order to verify the variables associated with mortality. Only data with a p-value <0,05 was considered. RESULTS: Out of the 65 patients who suffered an ischemic stroke included in the study, 50 were discharged and 15 died in hospital. Among the inflammatory markers, the neutrophil-tolymphocyte ratio (OR 1.55; p-value <0,01) was associated with a greater chance of death. Patients who died presented with higher levels of ultra-sensitive CRP, higher absolute monocyte count, lower lymphocyte-to-monocyte ratio and higher neutrophil-to- lymphocyte ratio. CONCLUSION: the elevated neutrophil-to-lymphocyte ratio may be significantly associated with negative outcomes following an ischemic stroke


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Ischemic Stroke/mortality , Ischemic Stroke/epidemiology , Inflammation/blood , Blood Cell Count , Comorbidity , Prevalence , Retrospective Studies , Risk Factors
3.
Rev. bras. med. esporte ; 27(3): 278-281, July-Sept. 2021. tab
Article in English | LILACS | ID: biblio-1288578

ABSTRACT

ABSTRACT Introduction Regular physical activity helps improve cardiovascular and cerebrovascular skills. How to evaluate the nervous tension of the cardiovascular and cerebrovascular vessels through sports is a hot topic. Objective The paper discusses the influence of regular participation in sports on people's cardiovascular function and blood-related indicators. Methods We select 30 healthy older adults who regularly participate in sports, record their ECG changes, blood pressure, heart rate and other related cardiovascular function indicators, and analyze the blood function of the elderly. Detection of blood cell count (RBC), red blood cell volume (MCV) and hemoglobin (Hb), serum creatinine (Cr), blood glucose (BGS), triglycerides (TG), cholesterol (TC), low-density lipoprotein (LDL) and high-Density lipoprotein (HDL) is measured. Results Older adults who persist in exercise for a long time have better indicators than those who do not exercise. Conclusions Appropriate aerobic exercise can reduce the stiffness of blood vessels in the elderly. Exercise can help the elderly increase heart rate variability and improve the heart's autonomic nerve function's blood indicators, and body mass. Level of evidence II; Therapeutic studies - investigation of treatment results.


RESUMO Introdução A atividade física regular ajuda a melhorar as habilidades cardiovasculares e cerebrovasculares. Como avaliar a tensão nervosa dos vasos cardiovasculares e cerebrovasculares por meio de esportes é um assunto quente. Objetivo o artigo discute a influência da participação regular em esportes sobre a função cardiovascular das pessoas e indicadores relacionados ao sangue. Métodos Selecionamos 30 idosos saudáveis que participam regularmente de esportes, registramos suas alterações no ECG, pressão arterial, frequência cardíaca e outros indicadores relacionados à função cardiovascular e analisamos a função sanguínea dos idosos. Detecção de contagem de células sanguíneas (RBC), volume de glóbulos vermelhos (MCV) e hemoglobina (Hb), creatinina sérica (Cr), glicose no sangue (BGS), triglicerídeos (TG), colesterol (TC), lipoproteína de baixa densidade (LDL ) e a lipoproteína de alta densidade (HDL) é medida. Resultados Idosos que persistem por muito tempo nos exercícios têm melhores indicadores do que aqueles que não praticam. Conclusão O exercício aeróbio adequado pode reduzir a rigidez dos vasos sanguíneos em idosos. O exercício pode ajudar os idosos a aumentar a variabilidade da frequência cardíaca e melhorar os indicadores sanguíneos da função nervosa autonômica do coração e a massa corporal. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción La actividad física regular ayuda a mejorar las habilidades cardiovasculares y cerebrovasculares. Cómo evaluar la tensión nerviosa de los vasos cardiovasculares y cerebrovasculares a través del deporte es un tema candente. Objetivo El artículo analiza la influencia de la participación regular en deportes sobre la función cardiovascular de las personas y los indicadores relacionados con la sangre. Métodos Seleccionamos a 30 adultos mayores sanos que participan regularmente en deportes, registramos sus cambios en el ECG, presión arterial, frecuencia cardíaca y otros indicadores relacionados con la función cardiovascular, y analizamos la función sanguínea de los ancianos. Detección del recuento de glóbulos rojos (RBC), volumen de glóbulos rojos (MCV) y hemoglobina (Hb), creatinina sérica (Cr), glucosa en sangre (BGS), triglicéridos (TG), colesterol (TC), lipoproteínas de baja densidad (LDL) ) y se mide la lipoproteína de alta densidad (HDL). Resultados Los adultos mayores que persisten en el ejercicio durante mucho tiempo tienen mejores indicadores que los que no lo hacen. Conclusión El ejercicio aeróbico adecuado puede reducir la rigidez de los vasos sanguíneos en los ancianos. El ejercicio puede ayudar a los ancianos a aumentar la variabilidad de la frecuencia cardíaca y mejorar los indicadores sanguíneos y la masa corporal de la función nerviosa autónoma del corazón. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento.


Subject(s)
Humans , Male , Female , Middle Aged , Aged , Aged, 80 and over , Sports/physiology , Blood Cell Count , Blood Pressure/physiology , Hemoglobins/analysis , Heart Rate/physiology , Lipids/blood
4.
Arq. bras. med. vet. zootec. (Online) ; 73(4): 791-798, Jul.-Aug. 2021. tab
Article in English | LILACS, VETINDEX | ID: biblio-1285265

ABSTRACT

The Nelore breed is the second largest bovine breed in the world and has actively participated in the expansion of new Brazilian agricultural frontiers. In this context, the purpose of this study was to determine the hematological and biochemical reference intervals of healthy Nelore matrices raised under an extensive regime without supplementation along southwest of Piauí state. Blood samples were collected from fifty-five multiparous female of the Nelore breed. Biochemical and hematological parameters were analyzed using a parametric statistical method with 95% CI of reference limits. The average values of red blood cells, hemoglobin as well as hematimetric indices showed reference ranges similar to reference standards. The hematocrit as well as granulocytes and agranulocytes presented alterations typical of animals raised in environments with higher temperatures. Mineral, enzymatic, protein and metabolic profiles were similar to other bovine breeds but with a narrower range of values. However, lower mean values were observed for levels of ionized calcium, total protein and urea. Nelore females present slightly different biochemical and hematological profiles from other breeds, which might result from the environmental and nutritional management applied and the natural deficiency of nitrogen, phosphorus and calcium in the region's pastures.(AU)


Nelore é a segunda maior raça bovina do mundo e tem participado ativamente da expansão das novas fronteiras agrícolas brasileiras. Nesse contexto, o objetivo do presente estudo foi determinar os intervalos de referência hematológicos e bioquímicos de matrizes Nelore criadas em regime extensivo sem suplementação, ao longo do sudoeste do estado do Piauí. Amostras de sangue foram coletadas de 55 fêmeas multíparas da raça Nelore. Os parâmetros bioquímicos e hematológicos foram analisados por método estatístico paramétrico com IC 95% para os limites de referência. Os valores médios de hemácias, hemoglobina e índices hematimétricos apresentaram intervalos de referência semelhantes aos padrões de referência. Tanto o hematócrito quanto os granulócitos e os agranulócitos apresentaram alterações típicas de animais criados em ambientes com temperaturas mais elevadas. Os perfis mineral, enzimático, proteico e metabólico foram semelhantes aos de outras raças bovinas, mas com uma faixa de valores mais estreita. No entanto, valores médios mais baixos foram observados para os níveis de cálcio ionizado, proteína total e ureia. Fêmeas Nelore apresentam perfis bioquímicos e hematológicos ligeiramente diferentes de outras raças, o que pode resultar dos manejos ambiental e nutricional aplicados e da deficiência natural de nitrogênio, fósforo e cálcio nas pastagens da região.(AU)


Subject(s)
Animals , Female , Cattle , Blood Cell Count/veterinary , Blood Urea Nitrogen , Blood Proteins/analysis , Calcium/blood , Granulocytes , Hematocrit/veterinary , Reference Values , Brazil , Serum , Agranulocytosis/veterinary
5.
J. health inform ; 13(2): 49-56, 20210000. []
Article in English | LILACS | ID: biblio-1359327

ABSTRACT

Objective: Present an explainable artificial intelligence (AI) approach for COVID-19 diagnosis with blood cell count. Methods: Five AI algorithms were evaluated: Logistic Regression, Random Forest, Support Vector Machine, Gradient Boosting and eXtreme Gradient Boosting. A Bayesian optimization with 5-Fold cross-validation was used to hyper-parameters tuning. The model selection evaluated three results: cross validation performance, test set prediction performance and a backtest: performance on identifying patients negative for COVID-19, but positive for others respiratory pathologies. Shapley Additive explanations (SHAP) was used to explain the chosen model. Results: A Random Forest model was obtained with 77.7% F1-Score (IC95%:57.1;92.3), 85.9% AUC (IC95%:73.7;95.9), 74.4% Sensitivity (IC95%:50.0;92.1) and 97.5% Specificity (IC95%:93.6;100.0). The main features were leukocytes, platelets and eosinophils. Conclusion: The research highlights the importance of model interpretability, demonstrating blood cell count as a possibility for COVID-19 diagnosis. The methodological structure developed, using TRIPOD's guidelines, can be extrapolated to other pathologies.


Objetivo: Propor uma abordagem com inteligência artificial explicável para diagnóstico de COVID-19 com hemograma. Métodos: Cinco algoritmos de IA foram testados: Regressão Logística, Florestas Aleatórias, Máquina de Vetores de Suporte, Gradient Boosting e eXtreme Gradient Boosting. Os hiper-parâmetros foram definidos através da otimização bayesiana com validação cruzada 5-Fold. A seleção de modelo utilizou três resultados de desempenho para definir o melhor modelo: validação cruzada, conjunto de teste e rendimento na identificação de pacientes negativos para COVID-19, porém positivos para outras patologias respiratórias (backtest). Ao final, Shapley Additive explanations (SHAP) foi utilizado para explicar o modelo escolhido. Resultados: Obteve-se um modelo Random Forest com F1-Score de 77.7% (IC95%:57.1;92.3), AUC de 85.9% (IC95%:73.7;95.9), Sensibilidade de 74.4% (IC95%:50.0;92.1) e Especificidade de 97.5% (IC95%:93.6;100.0). As principais variáveis foram leucócitos, plaquetas e eosinófilos. Conclusão: A pesquisa destaca a importância da interpretabilidade do modelo, demonstrando o hemograma como uma possibilidade para diagnosticar COVID-19. A estrutura metodológica desenvolvida no estudo, utilizando as diretrizes do TRIPOD, pode ser extrapolada para detecção de outras patologias.


Objetivo: Proponer un enfoque explicable de inteligencia artificial (IA) para el diagnóstico de COVID-19 con el uso de hemograma. Métodos: Cinco modelos de IA fueron evaluados: Logistic Regression, Random Forest, Support Vector Machine, Gradient Boosting e eXtreme Gradient Boosting. Los hiper-parámetros fueron definidos a través de optimización bayesiana con validación cruzada 5-Folds. La selección del modelo se utilizó tres resultados: rendimiento del validación cruzada, rendimento en conjunto de pruebas y el análisis de desempeño en identificación de pacientes negativos para COVID-19, pero positivos para otras patologías respiratorias (backtest). Shapley Additive explanations (SHAP) fue utilizado para explicar el modelo elegido. Resultados: Se obtuvo un modelo Random Forest con F1-Score de 77.7% (IC95%:57.1;92.3), AUC de 85.9% (IC95%:73.7;95.9), Sensibilidad de 74.4% (IC95%:50.0;92.1) y Especificidad de 97.5% (IC95%:93.6;100.0). Las principales variables fueron leucocitos, plaquetas y eosinófilos. Conclusión: La investigación presenta la importancia de la interpretabilidad del modelo, demostrando el uso de hemograma como posibilidad para diagnosticar COVID-19. La estructura elaborada, siguiendo las directrices de TRIPOD, puede ser extrapolar para otras patologías.


Subject(s)
Humans , Respiratory Tract Diseases/diagnosis , Blood Cell Count/methods , Artificial Intelligence , COVID-19/diagnosis
6.
Artrosc. (B. Aires) ; 28(1): 81-86, 2021.
Article in Spanish | LILACS, BINACIS | ID: biblio-1252455

ABSTRACT

Introducción: El objetivo del siguiente estudio fue evaluar la influencia de la edad, el sexo, el índice de masa corporal y el hemograma previo de los pacientes en la composición final del PRP. Materiales y métodos: Se realizó un análisis multivariado en trescientos pacientes para determinar la relación entre las mencionadas variables. Resultados: el número de plaquetas finales mostró una relación directa con las plaquetas basales (F (9.187) = 2.067, p = 0.034). Cuando las plaquetas basales aumentaron en una unidad, las plaquetas finales aumentan en promedio con un intervalo de valores de 0.236 a 4.618 (IC 95%, p = 0.00752). Discusión: el valor esperado medio de leucocitos para el protocolo "bajo" es de 1597 y para el nivel "rico" es de 9253, con un IC 95% se espera que el protocolo "rico" aumente en promedio la cantidad de leucocitos finales entre 280 y 785 % con respecto al protocolo "bajo" (p <0.001). Ninguna de las otras variables estudiadas tuvo una influencia significativa en la composición final del PRP. Conclusión: la cantidad de plaquetas en el hemograma basal afectó significativamente la concentración final de plaquetas en el PRP. Además, el protocolo de preparación afectó la concentración final de leucocitos, la que fue significativamente mayor en el protocolo de PRP rico en leucocitos que en el protocolo pobre en leucocitos. El sexo, el IMC y el HTO previo no influyeron significativamente en las concentraciones finales de plaquetas ni de leucocitos del PRP final. Nivel de Evidencia: IV


Introduction: The purpose of this study study was to evaluate the influence of age, sex, body mass index and previous blood count of patients on the final composition of the PRP. Material and methods: a multivariate analysis was performed in three hundred patients to determine the relationship between sex, age, body mass index (BMI) and the characteristics of the baseline blood count with the final composition of the PRP. Results: the number of final platelets showed a direct relationship with the basal platelets (F (9.187) = 2.067, p = 0.034). When the basal platelets increased by one unit, the final platelets increased on average with a range of values from 0.236 to 4.618 (95% CI, p = 0.00752).Discussion: the mean expected value of leukocytes for the "low" protocol is 1597 and for the "rich" level it was 9253, with a 95% CI the "rich" protocol is expected to increase on average the amount of final leukocytes between 280 and 785 % with respect to the "low" protocol (p <0.001). None of the other variables studied had a significant influence on the final composition of the PRP. Conclusion: the amount of platelets in the basal blood count significantly affected the final concentration of platelets in the PRP. Likewise, the preparation protocol affected the final leukocyte concentration, being the same significantly higher in the leukocyte-rich PRP protocol than in the leukocyte-poor protocol. Sex, BMI and previous HTO did not significantly influence the final platelet or leukocyte concentrations of the final PRP. Level of Evidence: IV


Subject(s)
Blood Cell Count , Body Mass Index , Musculoskeletal Diseases , Platelet-Rich Plasma
7.
Int. j. morphol ; 38(6): 1618-1622, Dec. 2020. tab, graf
Article in English | LILACS | ID: biblio-1134488

ABSTRACT

SUMMARY: The use of hematological counts for the prevention, diagnosis and follow-up of hematological diseases has increased. Indeed, the correct operation of a clinical laboratory is essential to producing comparable results. However, there is a paucity of validation and reproducibility studies among the different existing methods for clinical analysis. Therefore, our aim was to assess the commutability of the results provided by analyzers with different measuring systems. Sixty venous blood samples were obtained from patients, without discriminating for age or sex. Then, an automated hematological analysis was performed using the Cell-Dyn Ruby and HumaCount 5L instruments. The variables measured were: RBC, Hb, HCT, MCV, MCH and MCHC. The data were compared by a one-way ANOVA and Pearson's correlation coefficient. Statistical significance was fixed at p < 0.05. There were no statistically significant differences for RBC, HCT, MCH or MCHC. In addition, with the exception of MCHC, all the analytes showed a good correlation coefficient between the two instruments. There is a variety of automated systems for the clinical laboratory and it is essential for the clinician to know the different methodologies used in hematological analyzers as well as their sensitivity and specificity. Therefore, our results are useful for demonstrating the importance of practical knowledge of the analyzers mentioned.


RESUMEN: El uso de recuentos de células sanguíneas para la prevención, diagnóstico y monitoreo de enfermedades hematológicas ha ido en aumento. Por ello, el funcionamiento correcto de un laboratorio clínico es indispensable para producir resultados comparables. Sin embargo, existen pocos estudios de validación y reproducibilidad de los diferentes métodos de análisis clínico existentes. Por lo tanto, nuestro objetivo fue evaluar la intercambiabilidad de los resultados entregados por los analizadores que utilizan diferentes sistemas de medición. Se obtuvieron sesenta muestras de sangre venosa de pacientes, sin discriminar por edad o sexo. Los eritrogramas fueron obtenidos utilizando los analizadores automatizados Cell-Dyn Ruby y HumaCount 5L. Las variables medidas fueron: RBC, Hb, HCT, MCV, MCH y MCHC. Los datos fueron comparados por ANOVA a una vía y la correlación de Pearson. La significación estadística se estableció en el nivel estándar p<0,05. No hubo diferencias estadísticamente sig- nificativas para RBC, HCT, MCH y MCHC. Con la excepción de la MCHC, todos los analitos presentaron un buen coeficiente de correlación entre los dos analizadores comparados. Existen varios sistemas de automatización para su uso en laboratorios clínicos. Por lo tanto, es primordial para el clínico estar familiarizado con las diferentes metodologías utilizadas en los analizadores de sangre, así como su sensibilidad y especificidad. Nuestros resultados son útiles para mostrar la importancia del conocimiento práctico de los diferentes sistemas de medidas comparados.


Subject(s)
Humans , Hematologic Diseases/diagnosis , Hematologic Tests/methods , Blood Cell Count/methods , Blood Cells , Hemoglobins , Reproducibility of Results , Analysis of Variance , Sensitivity and Specificity , Erythrocyte Indices , Flow Cytometry , Hematocrit
8.
Rev. méd. panacea ; 9(2): 82-89, mayo-ago. 2020. tab, graf
Article in Spanish | LIPECS, LILACS, LIPECS | ID: biblio-1121154

ABSTRACT

Objetivo: Describir los hallazgos hematológicos en donantes preseleccionados a plaquetoferesis sanguínea en el Hospital Nacional Edgardo Rebagliati Martins (HNERM) de Lima, Perú. Materiales y métodos: Estudio de tipo observacional, descriptivo y transversal, realizado entre Mayo-Julio 2019. Se incluyeron hemogramas de donantes que resultaron ̈Aptos ̈ a la entrevista médica, con resultado ̈Negativo ̈ a marcadores serológicos y que fueron diferidos en donar por presentar alteración hematológica. Los datos fueron recolectados del sistema informático del servicio de medicina transfusional del HNERM. Se utilizó estadística descriptiva y analítica (U de Mann Whitney y Chi-cuadrado de Pearson) considerándose un valor p<0,05 como significativo. Resultados: Se analizaron los hemogramas de 330 donantes preseleccionados diferidos, entre los cuales el recuento absoluto de eosinófilos (14,2%), el hematocrito (14,2%) y el recuento de plaquetas (33,9%) fueron las de mayor presencia. Asimismo, en las observaciones la fórmula leucocitaria invertida (20,6%) fue la más frecuente. Además, se observó diferencia estadística significativa (p<0,05) entre la hemoglobina, el hematocrito, la hemoglobina corpuscular media (HCM) y la fórmula leucocitaria invertida de acuerdo al sexo del donante. Conclusiones: La alteración hematológica observada más frecuentemente en esta población fue la plaquetopenia. Además, algunos parámetros de la serie roja y la distribución leucocitaria en el hemograma estuvieron asociados con el género del donante. (AU)


Objective: To describe the hematological findings in preselected donors to blood plateletpheresis at the Edgardo Rebagliati Martins National Hospital (HNERM) in Lima, Peru. Materials and methods: Observational, descriptive and cross-sectional study, carried out between May-July 2019. Blood counts were included from donors that were ̈Approved ̈ to the medical interview, with a ̈Negative ̈ result for serological markers and that were deferred in donating for present hematological alteration. The data was collected from the computer system of the HNERM transfusion medicine service. Descriptive and analytical statistics (Mann Whitney's U and Pearsonʼs Chi-square) were used, considering a p<0.05 as significant. Results: The blood counts of 330 delayed preselected donors were analyzed, among which the absolute eosinophil count (14.2%), the hematocrit (14.2%) and the platelet count (33.9%) were those with the highest presence. Likewise, in observations, the inverted white blood cell formula (20.6%) was the most frequent. In addition, a statistically significant difference (p<0.05) was observed between hemoglobin, hematocrit, mean corpuscular hemoglobin (MCH) and the inverted leukocyte formula according to the sex of the donor. Conclusions: The hematological alteration most frequently observed in this population was plateletpenia. In addition, some parameters of the red series and the leukocyte distribution on the blood count were associated with the gender of the donor. (AU)


Subject(s)
Humans , Male , Female , Blood Cell Count , Blood Donors , Plateletpheresis , Hematologic Diseases , Epidemiology, Descriptive , Cross-Sectional Studies , Observational Studies as Topic
9.
Medicina (Ribeirao Preto) ; 53(2)jul. 2020. ilus, tab
Article in Portuguese | LILACS | ID: biblio-1358082

ABSTRACT

RESUMO: Introdução: As doenças cardiovasculares(DCV)estão entre as principais causas de mortalidade entre idosos, sendo a dislipidemia um importante fator de risco. Além disso, a relação Triglicerídeo/HDL-C e a razão Neutrófilos/Linfócitos apresentam estreita relação com os fatores de riscos para DCV. Objetivo: Comparar as relações Triglicerídeo/HDL-C e Neutrófilos/Linfócitos na população idosa sem e com dislipidemia. Métodos: Estudo documental, transversal, retrospectivo, com abordagem quantitativa e comparativa. A amostra foi de conveniência e incluiu os 110 idosos residentes em uma Instituição de Longa Permanência de Idosos. Foram coletados dados do perfil lipídico, hemograma, idade e sexo. Os idosos foram divididos em dois grupos: Com Dislipidemia e Sem Dislipidemia. As relações Triglicerídeo/HDL-C e Neutrófilos/Linfócitos foram calculadas e comparadas. Os resultados foram apresentados como mediana e intervalo interquartil e analisados pelo teste de Mann-Whitney. As variáveis categóricas foram representadas como frequência absoluta (n) e relativa (%) e avaliadas pelo teste de Qui-Quadrado (X2). Utilizou-se o programa estatístico SPSS 20.0®e nível de significância < 0,05. Resultados: Foram selecionados 84 idosos com resultados do perfil lipídico e hemograma, sendo 56 (67%) idosos do sexo feminino e 28 (33%) do sexo masculino, com idade mediana de 75 (69-81) anos. O grupo Com Dislipidemia apresentou valores superiores para Triglicerídeo/HDL-C e para a relação Neutrófilos/Linfócitos não houve diferença estatística. Conclusão: A relação Triglicerídeo/HDL-C pode ser utilizada como uma medida preventiva de doenças cardiovasculares nos idosos participantes do estudo. Porém, a relação Neutrófilos/Linfócitos deve ser interpretada com cautela nos idosos que apresentam características particulares quanto ao desenvolvimento das DCV. (AU)


ABSTRACT: Introduction: Cardiovascular diseases (CVD) are the leading causes of mortality among the elderly, and dyslipidemia is an important risk factor. Moreover, the Triglyceride/HDL-C and Neutrophils/Lymphocytes ratios are closely related to CVD risk factors. Objective: Comparing the Triglyceride/HDL-C and Neutrophils/Lymphocytes ratios in elderlies' groups, with and without dyslipidemia. Methods: Documentary, cross-sectional, and retrospective study with a quantitative and comparative approach. The sample was of convenience and included 110 elderlies' residents in a Long-Term Care for Elderlies. Data on lipid profile, full blood count, age, and sex were obtained and the residents divided into two groups, With Dyslipidemia and Without Dyslipidemia. The Triglyceride/HDL-C and Neutrophils/Lymphocytes ratios for both groups were measured and compared. Results were presented as median and interquartile range and analyzed by the Mann-Whitney test. Categorical variables were represented as absolute (n) and relative (%) frequencies and evaluated by the Chi-square (X2). The statistical program SPSS 20.0® was used with a significance level p < 0,05. Results: Eighty-four elderlies with results of lipid profile and blood count were selected, 56 (67%) were female and 28 (33%) male, with a median age of 75 (69-81) years. The group With Dyslipidemia showed higher values for Triglyceride/HDL-C and Neutrophils/Lymphocytes ratios, there was no statistical difference between the groups studied. Conclusion: The Triglyceride/HDL-C ratio can be used as a preventive measure of cardiovascular diseases in the elderlies' participants of the study. However, the Neutrophils/Lymphocytes ratio should be interpreted with caution in the elderly, who have particular characteristics regarding the development of cardiovascular diseases. (AU)


Subject(s)
Humans , Male , Female , Aged , Blood Cell Count , Biomarkers , Cardiovascular Diseases , Chi-Square Distribution , Risk Factors , Dyslipidemias
10.
Rev. bras. anal. clin ; 52(2): 131-137, 20200630.
Article in Portuguese | LILACS | ID: biblio-1146823

ABSTRACT

A COVID-19 se manifesta principalmente como uma infecção do trato respiratório. Entretanto, uma enorme quantidade de estudos mostra características de uma enfermidade sistêmica com repercussões nos sistemas cardiovascular, respiratório, gastro­intestinal, neurológico, hematopoiético e imunológico. Os estudos realizados em vários centros de pesquisa na China, Europa e nos Estados Unidos indicam que os resultados laboratoriais podem fornecer à equipe clínica muitos marcadores prognósticos de grande utilidade. O impacto no sistema hematopoiético e na hemostasia é evidenciado por alterações importantes na quantidade de linfócitos, granulócitos e plaquetas além de alterações no processo de coagulação. Estes parâmetros podem ser monitorados e têm efeito prognóstico na evolução da doença podendo ajudar a identificar pacientes que necessitem de cuidados intensivos. Em resumo, a COVID-19 apresenta alterações importantes do sistema hematopoiético estando frequentemente associada a um estado de hipercoagulabilidade. A avaliação cuidadosa dos índices laboratoriais no início da doença e durante a evolução podem ajudar o corpo clínico a formular uma abordagem de tratamento adaptada à situação além de permitir atenção especial àqueles pacientes que apresentam maior necessidade.


COVID-19 manifests itself mainly as an infection of the respiratory tract. However, a huge number of studies show characteristics of a systemic disease with repercussions on the cardiovascular, respiratory, gastrointestinal, neurological, hematopoietic and immunological systems. Studies carried out in various research centers in China, Europe and the United States indicate that laboratory results can provide the clinical team with many useful prognostic markers. The impact on the hematopoietic system and hemostasis is evidenced by important changes in the amount of lymphocytes, granulocytes and platelets, in addition to changes in the coagulation process. These parameters can be monitored and have a prognostic effect on the evolution of the disease and can help to identify patients who need intensive care. In summary, COVID-19 presents important changes in the hematopoietic system and is frequently associated with a state of hypercoagulability. Careful assessment of laboratory indexes at the onset of the disease and during evolution can help the clinical staff to formulate a treatment approach adapted to the situation, in addition to allowing special attention to those most severe patients.


Subject(s)
Thrombocytopenia , Blood Cell Count , Blood Coagulation , Coronavirus Infections , Coronavirus , Severe Acute Respiratory Syndrome , Disseminated Intravascular Coagulation , Betacoronavirus , Leukopenia , Neutrophils
11.
Autops. Case Rep ; 10(2): e2020147, Apr.-June 2020. graf
Article in English | LILACS | ID: biblio-1131811

ABSTRACT

In adults, B-lymphocytes comprise approximately 10% of circulating lymphocytes. The majority of peripheral B cells are B2 cells ("Mature" B-cells), which function as part of the humoral adaptive immune system. B1 cells ("Innate-like" B cells) are another sub-class of B lymphocytes, considered as innate immune cells with a characteristic phenotype (CD20+, CD27+, CD43+, CD70-, CD11b+, sIgM++, sIgD+) which can be divided into two subtypes; B1a (CD5+): spontaneously produce broadly reactive natural IgM, and B1b (CD5-): can generate T-cell independent, long-lasting IgM. There is very limited data available, indicating a correlation between allogeneic bone marrow transplantation and an increase in B1a cells. Here we present a case of a 17-year-old female with homozygous sickle cell disease (HbSS disease) who underwent hematopoietic stem cell transplant (HSCT). Approximately seven months post-transplant, she was found to have 16% immature mononuclear cells on complete blood count (CBC)-differential report. A follow-up peripheral blood flow cytometry showed that these cells were polyclonal CD5+/CD20+ B-cells, and comprised 66% of lymphocytes. Further workup and follow up failed to reveal any lymphoproliferative disorders. It is important not to misdiagnose these cells as an atypical CD5+ lymphoproliferative disorder. The presence of B1a cells has not been widely reported in non-neoplastic post-stem cell transplanted patients. This case also adds to and expands our knowledge regarding the presence of increased circulating B1a cells after stem cell transplant in a patient with no history of hematological malignancy.


Subject(s)
Humans , Female , Adolescent , Stem Cell Transplantation/adverse effects , Blood Cell Count , Hematopoietic Stem Cells , B-Lymphocytes/cytology , B-Lymphocyte Subsets/pathology , Flow Cytometry , Anemia, Sickle Cell , Lymphoproliferative Disorders/diagnosis
12.
Rev. cuba. hematol. inmunol. hemoter ; 36(1): e1090, ene.-mar. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1126545

ABSTRACT

Introducción: El cordón umbilical se ha convertido en un elemento de interés para la medicina regenerativa en los últimos años, pues constituye una fuente importante de células madres y progenitores hematopoyéticos. Objetivo: Caracterizar morfológicamente la sangre del cordón umbilical para terapia regenerativa en recién nacidos del Hospital Universitario Ginecobstétrico Ana Betancourt de Mora. Métodos: Estudio observacional, analítico y transversal realizado en el Centro de Inmunología y Productos Biológicos de Camagüey, entre enero y diciembre de 2017. Se evaluaron 35 muestras de sangre del cordón umbilical obtenidas de recién nacidos, que fueron partos eutócicos y sus madres no tuvieron procesos de enfermedades infecciosas. Resultados: El promedio mayor de células mononucleares correspondió a los linfocitos. En el conteo diferencial los polimorfonucleares neutrófilos ocuparon el primer lugar, seguido de los linfocitos, con medias de 0,50 y 0,46 x 109/L, respectivamente. De las células presentes en el frotis del botón, fueron más frecuente los linfocitos con 0.59 x 109/L; se observó un promedio de monocitos de 0,00-0,07 x 109/L. Conclusiones: La obtención de células mononucleares viables a través de la vena umbilical, constituye una técnica promisoria en las investigaciones biomédicas. Entre las células mononucleares predominaron los linfocitos, tanto en la sangre del cordón umbilical como en el botón celular aislado(AU)


Introduction: In recent years, the umbilical cord has become an element of interest for regenerative medicine, based on its importance as a source of stem cells and hematopoietic progenitors. Objective: To characterize the morphology of umbilical cord blood for regenerative therapy in newborns from Ana Betancourt de Mora Gyneco-obstetric University Hospital. Methods: Observational, analytical and cross-sectional study carried out at the Center of Immunology and Biological Products in Camagüey, between January and December 2017. We evaluated 35 samples of umbilical cord blood obtained from newborns who were eutocic deliveries and whose mothers did not have infectious disease processes. Results: The highest average of mononuclear cells corresponded to lymphocytes. In the differential count, neutrophil polymorphonuclear cells ranked first, followed by lymphocytes, with averages of 0.50x109 and 0.46x109 per liter, respectively. Of the cells present in the button cell smear, lymphocytes were more frequent, with 0.59x109 per L; an average of monocytes was observed, with 0.00-0.07x109 per L was observed. Conclusions: Obtaining viable mononuclear cells through the umbilical vein is a promising technique in biomedical research. Among the mononuclear cells, lymphocytes predominated, both in the cord blood and in the isolated cell button(AU)


Subject(s)
Humans , Male , Female , Cord Blood Stem Cell Transplantation/methods , Regenerative Medicine/methods , Blood Cell Count/standards , Cross-Sectional Studies
13.
Rev. Assoc. Med. Bras. (1992) ; 66(2): 133-138, Feb. 2020. tab, graf
Article in English | SES-SP, LILACS, SES-SP | ID: biblio-1136183

ABSTRACT

SUMMARY OBJECTIVE Periodontitis may stimulate infectious and immune response and cause the development of atherogenesis, coronary heart disease, and myocardial infarction. The aim of this study was to compare the plateletcrit (PCT) and mean platelet volume (MPV) levels derived from complete blood count (CBC) tests in patients suffering from stage 3 periodontitis with those of healthy individuals without periodontal disease. METHODS The study included 57 patients (28 females and 29 males) with Stage 3 Periodontitis and 57 volunteering individuals (31 females and 26 males) who were periodontally healthy. The age of study participants ranged from 18 to 50 years. Their periodontal condition was investigated with probing depth (PD), clinical attachment level, bleeding on probing, and plaque index. Leukocyte (WBC) and erythrocyte count (RBC), hemoglobin (Hb) and hematocrit (HCT) levels, mean corpuscular volume (MCV) and red cell distribution width (RDW), thrombocyte count, mean platelet volume (MPV), plateletcrit (PCT ), and neutrophil and lymphocyte counts were evaluated based on the CBC test results of the study participants. RESULTS PCT, WBC, Neutrophil, and MPV values were found to be significantly higher in the periodontitis group (p<0.05). There were no significant differences in RBC counts, Hb, HCT, MCV, RDW, and platelet and lymphocyte counts between the two study groups (p>0.05). CONCLUSIONS PCT and MPV levels may be a more useful marker to determine an increased thrombotic state and inflammatory response in periodontal diseases.


RESUMO OBJETIVO A periodontite pode estimular a resposta infecciosa e imunitária e causar o desenvolvimento da aterogênese, doença coronária e infarto do miocárdio. O objetivo deste estudo foi comparar os níveis de plaquetócrito (PCT) e de volume médio de plaquetas (VMP) derivados dos testes de hemograma completo (CBC) em doentes que sofrem de periodontite de fase 3 com os de indivíduos saudáveis, sem doença periodontal. MÉTODOS O estudo incluiu 57 doentes (28 mulheres e 29 homens) com periodontite de fase 3 e 57 voluntários (31 mulheres e 26 homens) que eram periodontalmente saudáveis. A idade dos participantes do estudo variou de 18 a 50 anos. A condição periodontal dos participantes do estudo foi investigada com profundidade de sonda (PD), nível de ligação clínica, hemorragia na sonda e índice de placas. Contagem de leucócitos (WBC) e eritrócitos (RBC), níveis de hemoglobina (Hb) e hematócrito (HCT), volume corpuscular médio (VCM) e largura de distribuição das células vermelhas (RDW), contagem de trombócitos, volume plaquetário médio (MPV), plaquetócrito (PCT) e contagem de neutrófilos e linfócitos foram avaliados com base nos resultados do teste CBC dos participantes do estudo. RESULTADO Verificou-se que os valores de PCT, WBC, neutrófilos e MPV eram significativamente mais elevados no grupo da periodontite (p<0,05). Não houve diferenças significativas nas contagens de glóbulos vermelhos, Hb, HCT, MCV, RDW; nem nas contagens de plaquetas e linfócitos entre os dois grupos estudados (p>0, 05). CONCLUSÃO Os níveis de PCT e MPV podem ser um marcador mais útil para determinar um estado trombótico aumentado e a resposta inflamatória em doenças periodontais.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Young Adult , Periodontitis/blood , Blood Platelets/cytology , Mean Platelet Volume , Reference Values , Blood Cell Count , Case-Control Studies , Periodontal Index , Cross-Sectional Studies , Statistics, Nonparametric , Middle Aged
14.
Rev. Assoc. Med. Bras. (1992) ; 66(2): 160-165, Feb. 2020. tab
Article in English | SES-SP, LILACS, SES-SP | ID: biblio-1136172

ABSTRACT

SUMMARY OBJECTIVE Coronary collateral development (CCD) predicts the severity of coronary heart disease. Hemogram parameters, such as mean platelet volume (MPV), eosinophil, red cell distribution width, and platelet distribution width (PDW), are supposed novel inflammatory markers. We aimed to compare hemogram parameter values in patients presenting with non-ST-elevation myocardial infarction (NSTEMI) with adequate or inadequate CCD. METHODS A total of 177 patients with NSTEMI undergoing coronary arteriography were enrolled and divided into two groups based on the development of CCD: one group with adequate CCD (n=88) and the other with impaired CCD (n=89). RESULTS Baseline demographics and clinical risk factors were similar between the groups. Hemogram parameters were not significantly different between the two groups. However, compared to the inadequate CCD group, the median PDW was significantly higher in the adequate CCD group, 17.6 (1.4) vs. 17.8 (1.6) p=0.004. In a multivariate analysis, PDW (p=0.001, 95% CI for OR: 0.489(0,319-0,750) was found to be significantly different in the adequate CCD group compared to the inadequate CCD group. Pearson's correlation analysis revealed that PDW was significantly correlated with the Rentrop score (r=0.26, p<0.001). CONCLUSIONS We suggest that since PDW is an index that is inexpensive and easy to assess, it could serve as a marker of CCD in patients with NSTEMI.


RESUMO OBJETIVO O desenvolvimento colateral coronariano (CCD) prediz a gravidade da doença coronariana. Parâmetros de hemograma como volume plaquetário médio (VPM), eosinófilo, largura de distribuição de glóbulos vermelhos e largura de distribuição de plaquetas (PDW) são supostos novos marcadores inflamatórios. Nosso objetivo foi comparar os valores do parâmetro hemograma em pacientes com infarto do miocárdio sem supradesnivelamento do segmento ST (IAMSSST) com DCC adequado ou inadequado. MÉTODOS Um total de 177 pacientes com NSTEMI submetidos à arteriografia coronariana foram incluídos e divididos, com base no desenvolvimento de CCD, em dois grupos: grupo com CCD adequado (n = 88) e grupo com CCD alterado (n = 89). RESULTADOS Os dados demográficos e os fatores de risco clínicos basais foram semelhantes entre os grupos. Os parâmetros do hemograma não foram significativamente diferentes entre os dois grupos. Mas, em comparação com a mediana inadequada do grupo CCD, o PDW foi significativamente maior em CCD adequado de 17,6 (1,4) vs. 17,8 (1,6) p = 0,004. Na análise multivariada, PDW (p = 0,001, IC 95% para OR: 0,489 (0,319-0,750) foi significativamente diferente no grupo CCD adequado em comparação com o grupo CCD inadequado. A análise de correlação de Pearson revelou que PDW foi significativamente correlacionado com escore de aluguel (r = 0,26, p <0,001). CONCLUSÃO Sugerimos que, uma vez que a PDW é um índice barato e de fácil avaliação, pode servir como um marcador de DCC em pacientes com IAMSSST.


Subject(s)
Humans , Male , Female , Aged , Aged, 80 and over , Coronary Artery Disease/physiopathology , Coronary Artery Disease/blood , Collateral Circulation/physiology , Coronary Circulation/physiology , Non-ST Elevated Myocardial Infarction/physiopathology , Non-ST Elevated Myocardial Infarction/blood , Reference Values , Blood Cell Count , Blood Platelets , Logistic Models , Multivariate Analysis , Retrospective Studies , Risk Factors , Coronary Angiography , Statistics, Nonparametric , Mean Platelet Volume , Middle Aged
15.
Med. lab ; 24(2): 131-140, 2020.
Article in Spanish | LILACS, COLNAL | ID: biblio-1097088

ABSTRACT

El EDTA es el anticoagulante de elección en los laboratorios de hematología para la conservación de la muestra de sangre total. Existen dos tipos, EDTA K2 y EDTA K3, y su diferencia radica en la cantidad de moléculas de potasio. Algunas guías sugieren que hay diferencias entre el anticoagulante EDTA K2 y el K3 para el proceso del hemograma; sin embargo, con las nuevas presentaciones de los tubos que traen las casas comerciales, no se tiene claro si en realidad aún hay diferencia entre los dos anticoagulantes, y si esto puede alterar el resultado del hemograma, tanto en el resultado cuantitativo, como en el cualitativo. Objetivo. Comparar los recuentos leucocitarios, la hemoglobina, el hematocrito, el volumen corpuscular medio, las plaquetas y la morfología celular en muestras de sangre periférica con EDTA K2 y EDTA K3, en diferentes tiempos (0, 1 y 2 horas). Materiales y métodos. Se realizó un estudio cuasi-experimental, multivariado, multifactorial, que tiene como unidad de análisis la sangre anticoagulada con EDTA K2 y EDTA K3, extraída de 53 individuos a través de un muestreo no probabilístico por conveniencia. Resultados. Al comparar los resultados del estudio morfológico por medio del extendido de sangre periférica y los datos cuantitativos del hemograma, se encontró que no hay diferencias estadísticamente significativas usando EDTA K2 o K3. Conclusión. Se evidenció que el uso del EDTA K2 o EDTA K3 como anticoagulante de elección, procesando las muestras en un tiempo adecuado después de su recolección, no afecta los parámetros cuantitativos del hemograma automatizado ni los morfológicos.


EDTA is the anticoagulant of choice in hematology laboratories for the conservation of whole blood samples. There are two types, K2 EDTA and K3 EDTA, and their difference lies in the amount of potassium molecules. Some guidelines suggest that there are differences between K2 and K3 EDTA for the blood analysis process. However, with the new collection tubes offered by the commercial suppliers, it is not clear if in fact there is a difference between the two anticoagulants that would result in changes in blood parameters and cell morphology. Objective. To compare leukocyte counts, hemoglobin, hematocrit, mean corpuscular volume, platelets and cell morphology in peripheral blood samples collected with K2 EDTA and K3 EDTA, at different times (0, 1 and 2 hours). Materials and methods. A quasi-experimental, multivariate, multifactorial study was carried out, with anticoagulated blood as the unit of analysis, either with K2 EDTA or K3 EDTA, extracted from 53 subjects through a non-probabilistic sampling for convenience. Results. There was no statistically significant difference when comparing results of the peripheral blood smear and the quantitative hematological parameters using K2 or K3 EDTA. Conclusion. The use of either K2 EDTA or K3 EDTA as the anticoagulant of choice, when processing samples within a suitable time after their collection, proved equally satisfactory for both quantitative and morphological parameters


Subject(s)
Humans , Blood Cells , Blood Cell Count , Edetic Acid
17.
Article in Chinese | WPRIM | ID: wpr-828511

ABSTRACT

OBJECTIVE@#To identify the key biochemical indicators that affect the clinical type and outcomes of COVID-19 patients and explore the application of neutrophil/lymphocyte ratio (NLR) in COVID-19.@*METHODS@#Ninety-three patients with confirmed diagnosis of COVID-19 admitted in Ezhou Central Hospital from February to April in 2020 were analyzed. Among them, 43 patients were selected from Intensive Care Unit (ICU) with the diagnosis of critical type of COVID-19, and 50 cases of common type were selected from the Department of Respiratory Medicine. The baseline data, blood routine test and biochemical indexes of the patients were collected on the first day of admission. NLRs of the patients were calculated, and COX survival analysis according to the NLR 4-category method was performed. The patients' outcomes were analyzed with receiver operating curves (ROCs). The patients were divided into two groups according to NLR cutoff value for comparison of the biochemical indexes. Based on the patients' outcomes, NLR cutoff value classification and clinical classification, multiple binary logistics regression was performed to screen the key variables and explore their significance in COVID-19.@*RESULTS@#The NLR four-category method was not applicable for prognostic evaluation of the patients. The cut-off value of NLR for predict the prognosis of COVID-19 was 11.26, with a sensitivity of 0.903 and a specificity of 0.839; the laboratory indicators of the patients with NLR < 11.26 were similar to those in patients of the common type; the indicators were also similar between patients with NLR≥11.26 and those with critical type COVID-19. NLR, WBC, NEUT, PCT, DD, BUN, TNI, BNP, and LDH had significant effects on the clinical classification and outcome of the patients ( < 0.05); Cr, Ca, PH, and Lac had greater impact on the outcome of the patients ( < 0.05), while Na, PCO had greater impact on the clinical classification of the patients ( < 0.05).@*CONCLUSIONS@#NLR can be used as an important reference for clinical classification, prognostic assessment, and biochemical abnormalities of COVID-19. Patients of critical type more frequently have bacterial infection with more serious inflammatory reactions, severer heart, lung and kidney damages, and much higher levels of DD and LDH than those of the common type. NLR, NEUT, DD, TNI, BNP, LDH, Ca, PCT, PH, and Lac have obvious influence on the prognosis of COVID-19 and should be observed dynamically.


Subject(s)
Betacoronavirus , Blood Cell Count , Reference Standards , Coronavirus Infections , Blood , Diagnosis , Humans , Lymphocytes , Cell Biology , Neutrophils , Cell Biology , Pandemics , Pneumonia, Viral , Blood , Diagnosis , Prognosis , ROC Curve , Retrospective Studies , Severity of Illness Index
18.
Article in English | WPRIM | ID: wpr-880735

ABSTRACT

BACKGROUND@#The proportion of recurrences after discharge among patients with coronavirus disease 2019 (COVID-19) was reported to be between 9.1% and 31.0%. Little is known about this issue, however, so we performed a meta-analysis to summarize the demographical, clinical, and laboratorial characteristics of non-recurrence and recurrence groups.@*METHODS@#Comprehensive searches were conducted using eight electronic databases. Data regarding the demographic, clinical, and laboratorial characteristics of both recurrence and non-recurrence groups were extracted, and quantitative and qualitative analyses were conducted.@*RESULTS@#Ten studies involving 2071 COVID-19 cases were included in this analysis. The proportion of recurrence cases involving patients with COVID-19 was 17.65% (between 12.38% and 25.16%) while older patients were more likely to experience recurrence (weighted mean difference (WMD)=1.67, range between 0.08 and 3.26). The time from discharge to recurrence was 13.38 d (between 12.08 and 14.69 d). Patients were categorized as having moderate severity (odds ratio (OR)=2.69, range between 1.30 and 5.58), while those with clinical symptoms including cough (OR=5.52, range between 3.18 and 9.60), sputum production (OR=5.10, range between 2.60 and 9.97), headache (OR=3.57, range between 1.36 and 9.35), and dizziness (OR=3.17, range between 1.12 and 8.96) were more likely to be associated with recurrence. Patients presenting with bilateral pulmonary infiltration and decreased leucocyte, platelet, and CD4@*CONCLUSIONS@#The main factors associated with the recurrence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) after hospital discharge were older age, moderate severity, bilateral pulmonary infiltration, laboratory findings including decreased leucocytes, platelets, and CD4


Subject(s)
Age Factors , Blood Cell Count , CD4 Lymphocyte Count , COVID-19/pathology , Cough , Dizziness , Headache , Humans , Patient Discharge , Recurrence , Risk Factors
19.
Rev. cuba. hematol. inmunol. hemoter ; 35(4): e1070, oct.-dic. 2019.
Article in Spanish | LILACS, CUMED | ID: biblio-1093291

ABSTRACT

Introducción: La visión actual de las enfermedades por inmunodeficiencia primaria (IDP) incluye un número creciente de síndromes que están asociados con la desregulación inmune y la autoinmunidad como características predominantes. Las citopenias autoinmunes pueden ser el primer signo de desregulación que precede a la presentación clásica de inmunodeficiencia primaria, con infecciones recurrentes u oportunistas. El conocimiento de un espectro de enfermedades potencialmente involucradas (hematológicas, reumatológicas e inmunológicas) es crucial para la identificación de una cierta proporción de genotipos y fenotipos de otros diagnósticos descritos. También permitirá excluir desórdenes como lupus eritematoso sistémico, inmunodeficiencia variable común, síndrome linfoproliferativo autoinmune; así como realizar diagnósticos diferenciales noveles como la deficiencia de GATA2, deficiencia de CD27, deficiencia de sensibilidad a lipopolisacáridos, síndrome fosfoinositol-3-quinasa delta activada, inmunodeficiencia ligada a X con déficit de magnesio y otros. Objetivo: Proporcionar una sinopsis conceptual de la aparición de citopenias en las IDP con el propósito de actualizar el conocimiento actual sobre dicho tema y de aumentar la percepción, tanto de hematólogos como inmunólogos, en relación a la presentación de citopenias como manifestación de estas enfermedades. Métodos: Se revisaron artículos originales y de corte experimental publicados en la década 2009 - 2019, en algunas bases de datos de la Biblioteca Virtual de Salud (BVS) de Cuba. Conclusiones: Al igual que las formas benignas autolimitadas de citopenia autoimmune post o parainfecciosas, o la neutropenia autoimmune adquirida de la infancia, que generalmente ocurren independientemente de una IDP subyacente reconocida, muchas de las citopenias que acompañan a esta enfermedad (pero no todas) están mediadas por autoanticuerpos. Es esencial entonces, que los médicos valoren, ante la evidencia clara de citopenia, que esta puede ser autoinmune(AU)


Introduction: The current view of primary immunodeficiency diseases (IDP) includes an increasing number of syndromes that are associated with immune dysregulation and autoimmunity as predominant characteristics. Autoimmune cytopenias may be the first sign of dysregulation that precedes the classic presentation of primary immunodeficiency, with recurrent or opportunistic infections. The knowledge of a spectrum of potentially involved diseases (hematological, rheumatological and immunological) is crucial for the identification of a certain proportion of genotypes and phenotypes of other diagnoses described. It will also allow excluding disorders such as systemic lupus erythematosus, common variable immunodeficiency, autoimmune lymphoproliferative syndrome; as well as making novel differential diagnoses such as GATA2 deficiency, CD27 deficiency, lipopolysaccharide sensitivity deficiency, activated delta phosphoinositol-3-kinase syndrome, X-linked immunodeficiency with magnesium deficiency and others. Objective: This review provides a conceptual synopsis of the appearance of cytopenias in the IDPs with the purpose of updating current knowledge on this topic and increasing the perception, of both hematologists and immunologists, in relation to the presentation of cytopenias as manifestation of these diseases. Methodos: Original and experimental articles published in the 2009-2019 decade were reviewed in some databases of the Virtual Health Library (VHL) of Cuba. Conclusions: As the self-limited benign forms of post or parainfectious autoimmune cytopenia, or childhood acquired autoimmune neutropenia, which generally occur independently of a recognized underlying IDP, many of the cytopenias that accompany this disease (but not all) mediated by autoantibodies. It is essential, then, that doctors assess, given the clear evidence of cytopenia, that it may be autoimmune(AU)


Subject(s)
Humans , Male , Female , Blood Cell Count/methods , Primary Immunodeficiency Diseases/epidemiology , Autoimmune Diseases/epidemiology , Retrospective Studies , Primary Immunodeficiency Diseases/physiopathology
20.
Int. j. odontostomatol. (Print) ; 13(4): 452-457, dic. 2019. tab, graf
Article in Spanish | LILACS | ID: biblio-1056484

ABSTRACT

RESUMEN: La caries temprana de la infancia severa (CTI-S) es una enfermedad crónica que afecta a niños menores de 6 años, produce dolor, infección y destrucción de los tejidos dentales. El dolor que experimentan los niños con CTI-S puede llevar a hábitos alimenticios alterados que pueden causar deficiencias nutricionales. El objetivo fue evaluar los valores de hemograma en niños con CTI-S y compararlos con los valores normales de referencia para la edad. Se realizó un estudio retrospectivo observacional. Se analizaron las fichas y hemogramas de 47 niños con CTI-S, clasificados como ASA 1, atendidos bajo anestesia general en el Hospital de la Fuerza Aérea de Chile. Se tomaron en cuenta los valores del hemograma en relación a: Hematocrito, Hemoglobina y VCM. Se realizaron test descriptivos para las variables en estudio y se utilizó el testt para comparar los valores de hemograma con los valores normales de referencia. Se encontró una disminución de los valores de hematocrito en 4 pacientes (8,5 %) y una disminución del valor de VCM en 17 pacientes (36,7 %). Se encontraron diferencias significativas al comparar los promedios obtenidos en relación a hematocrito, VCM y hemoglobina en niños con CTI-S con el promedio de referencia (p <0,001). De acuerdo con los resultados obtenidos en este estudio, podemos concluir que los niños con caries temprana de la infancia severa, tienen alteraciones en los valores promedio de hemograma en relación a hematocrito, hemoglobina y VCM.


ABSTRACT: Severe early childhood caries (S-ECC) is a multifactorial chronic disease that affects children under 6 years of age, produces pain, infection and destruction of the dental tissues. The pain experienced by children with SECC may lead to altered eating habits that may cause nutritional deficiencies. The aim of this study was to evaluate the hemogram values in children with severe early childhood caries, and compare them with age population reference values. An observational retrospective study was carried out. We analyzed the medical records and their respective hemograms of 47 children with S-ECC, classified as ASA1, attended at the Chilean Air Force Hospital under general anesthesia. The hemogram values were taken into account in relation to: hematocrit, hemoglobin and mean corpuscular volume (MCV). Descriptive tests were carried out for the variables under study and the t-test was used to compare the hemogram values with the normal reference values. A decrease in hematocrit values was found in 4 patients (8.5 %) and a decrease in the value of MCV in 17 patients (36.7 %). Significant differences were found when comparing the averages obtained in relation to hematocrit, hemoglobin and MCV in children with S-ECC with the reference average (p <0.001). According to the results obtained, in this study, we can conclude that children with severe early childhood caries, have alterations in the average of hemogram values in relation to hematocrit, hemoglobin and MCV.


Subject(s)
Humans , Male , Female , Child , Dental Caries/etiology , Dental Caries/epidemiology , Blood Cell Count , Clinical Record , Chile/epidemiology , Retrospective Studies , Ethics Committees
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