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1.
Acta amaz ; 51(2): 145-155, jun. 2021.
Article in English | LILACS | ID: biblio-1353474

ABSTRACT

Os mecanismos de defesa bioquímica dos anfíbios envolvem secreções cutâneas de moléculas bioativas com atividade antimicrobiana. Este estudo avaliou a atividade in vitro de extratos metanólicos da secreção cutânea de duas espécies de anfíbios da família Bufonidae, Rhaebo guttatus e Rhinella marina, no controle dos patógenos Fusarium udum, Fusarium solani, Colletotrichum truncatum, Aspergillus flavus, Rhizoctonia solani, Macrophomina phaseolina e Calonectria pseudometrosideri. O extrato de R. guttatus inibiu o crescimento micelial de F. udum, F. solani, A. flavus e M. phaseolina em algumas concentrações testadas. O extrato de R. marina inibiu o crescimento micelial de C. truncatum na concentração de 0,5 mg mL- ¹, e inibiu o crescimento micelial de A. flavus nas concentrações de 0,1 e 0,5 mg mL- ¹, que foi semelhante à inibição pelo controle positivo. O extrato de R. marina também diminuiu a produção de microescleródios de R. solani nas concentrações de 0,2 e 0,3 mg mL- ¹. Além disso, os extratos inibiram a esporulação e germinação de conídios em graus variados. A inibição da formação de apressórios em C. truncatum pelos extratos de R. guttatus e R. marina foi de 85%­99% e 63%­100%, respectivamente. Nossos resultados demonstraram que o tratamento com extratos da secreção cutânea de R. guttatus e R. marina apresentou atividade antifúngica sobre os fitopatógenos estudados.(AU)


Subject(s)
Spores, Fungal , Bufo marinus , Bodily Secretions , Amphibians
2.
Rev. cuba. invest. bioméd ; 40(1): e884, ene.-mar. 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1289449

ABSTRACT

La actual pandemia de COVID-19 causada por el virus SARS-CoV-2, se caracteriza por una alta morbilidad y mortalidad. Algunos estudios han reportado que la frecuencia de ictus en pacientes infectados con el virus oscila entre un 5-20 por ciento. A pesar de estas cifras alarmantes, las vías por las cuales el virus llega al sistema nervioso central y los mecanismos fisiopatológicos por los que puede ocurrir un ictus en estos pacientes no han sido totalmente esclarecidos. Numerosos estudios han demostrado que la infección por SARS-CoV-2 está asociada a un estado protrombótico, capaz de causar un tromboembolismo arterial y venoso. Además, se ha reportado una respuesta inflamatoria exacerbada, con reclutamiento de células sanguíneas y una secreción desproporcionada de citoquinas proinflamatorias. También la hipoxia y fenómenos cardioembólicos han sido propuestos como posibles mecanismos. Es esencial definir con exactitud los mecanismos fisiopatológicos que vincula la infección por SARS-CoV-2 con la ocurrencia del ictus, con la finalidad de aplicar tratamientos más específicos y evitar futuras complicaciones(AU)


The actual Coronavirus Disease 2019 (COVID-19) infection is an ongoing pandemic, characterized by high morbidity and mortality produced by SARS-CoV-2 virus. Studies reported a stroke frequency around 5-20 percent in infected patients; however, SNC invasion and pathophysiological mechanisms related to stroke in COVID-19 patients are still unknown. Several studies have demonstrated that SARS-CoV-2 infection is linked to a prothrombotic state causing venous and arterial thromboembolism. Also, an overstated inflammatory response with recruitment of blood cells and disproportioned secretion of proinflammatory cytokines has been reported. Finally, cardioembolism and hypoxia have been proposed as surrogate mechanisms. It is essential to define the pathophysiological mechanisms of stroke during the infection in order to apply more specific treatments to avoid further stroke complications(AU)


Subject(s)
Humans , Cytokines , Bodily Secretions , Stroke , COVID-19 , Hypoxia
3.
Rev. cuba. invest. bioméd ; 40(supl.1): e1090, 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1289481

ABSTRACT

Introducción: Hasta el momento se han encontrado 27 virus que pueden provocar viremia en el semen humano. Para muchos de estos, faltan datos sobre la transmisión sexual y su repercusión sobre la fertilidad masculina; lo cual resalta las lagunas de conocimiento sobre la persistencia de los virus en los fluidos genitales, especialmente el semen. Objetivo: Realizar una revisión bibliográfica sobre la potencialidad del nuevo coronavirus de persistir en el semen, afectar las células reproductoras masculinas y por ende su fertilidad. Métodos: Se realizó una búsqueda bibliográfica en la base de datos PubMed utilizando los siguientes términos: virus and fertility; coronavirus and semen; coronavirus and testi y coronavirus and angiotensin. Se consultaron un total de 30 artículos originales, de los cuales 24 eran de los últimos 5 años. Conclusiones: La presencia de virus en el semen puede ser más común de lo que se entiende actualmente como potencialidad de daño reproductivo, y no se debe suponer que los virus tradicionales de transmisión no sexual están totalmente ausentes en las secreciones genitales. Los estudios sobre detección viral y persistencia del semen son beneficiosos para la práctica clínica y la salud pública, además de la trascendencia de dichos estudios en el desarrollo fetal, como es el SARS-CoV-2(AU)


Introduction: Twenty-seven viruses have so far been found which may cause viremia in human semen. In many of them, more data are required about sexual transmission and its impact on male fertility, pointing to knowledge gaps about the persistence of the virus in genital fluids, particularly in semen. Objective: Carry out a bibliographic review about the potential of the novel coronavirus to persist in semen, affecting male reproductive cells and hence their fertility. Methods: A bibliographic search was conducted in the database PubMed using the search terms "virus and fertility", coronavirus and semen, coronavirus and testis and "coronavirus and angiotensin". A total 30 original papers were consulted, of which 24 were from the last five years. Conclusions: Emergence of the novel coronavirus poses the question of its existence in semen and its capacity to affect reproduction. Presence of the virus in semen may be more common than what is currently understood as reproductive damage potential, and it should not be supposed that traditional non-sexually transmitted viruses are totally absent from genital secretions. Studies about virus detection and semen persistence are useful to medical practice and public health, as well as in relation to their impact on fetal development, as is the case with SARS-CoV-2(AU)


Subject(s)
Humans , Male , Reproduction , Bodily Secretions , Fertility , COVID-19
4.
Article in Chinese | WPRIM | ID: wpr-880839

ABSTRACT

OBJECTIVE@#To evaluate the effect of general anesthesia on postoperative melatonin secretion in 4-to 6-year-old children with snoring.@*METHODS@#Twenty children with snoring aged 4-6 years of either gender (ASA grade Ⅰ and Ⅱ) were selected for adenoidectomy.Before, during and 3 days after the operation, salivary melatonin levels of the children were measured at 11 selected time points (T1-T11).The illumination intensity and body temperature of the children were recorded at each time point of measurement.The sleep time of the children in 3 days after the operation was recorded, and postoperative pain scores (FLACC) and Riker and Rehabilitation Quality Rating Scale-15(QoR-15) scores were assessed.Sleep Apnea Life Quality Evaluation Questionnaire (OSA-18) was used to evaluate postoperative recovery of the children at 28 days after the operation.The incidence of major adverse events of the children during hospitalization was recorded.@*RESULTS@#No significant difference was found in baseline salivary melatonin level among the 20 children before the operation.Salivary melatonin level at 7 am after the operation (T8) was significantly lowered as compared with that before the surgery (T4)(@*CONCLUSIONS@#In preschool children with snoring, general anesthesia affects but does not inhibit melatonin secretion on the first night after surgery, and minor surgeries under general anesthesia in the morning do not cause significant changes in melatonin secretion to cause disturbance of the circadian rhythm in these children.


Subject(s)
Anesthesia, General/adverse effects , Bodily Secretions , Child , Child, Preschool , Circadian Rhythm , Humans , Melatonin , Snoring
5.
J. venom. anim. toxins incl. trop. dis ; 27: e20200105, 2021. tab, graf
Article in English | ID: biblio-1180822

ABSTRACT

Amphibians inhabit the terrestrial environment, a conquest achieved after several evolutionary steps, which were still insufficient to make them completely independent of the aquatic environment. These processes gave rise to many morphological and physiological changes, making their skin (and cutaneous secretion) rich in bioactive molecules. Among the tree frogs, the secretion is composed mainly of peptides; but alkaloids, proteins and steroids can also be found depending on the species. The most known class of biologically active molecules is the antimicrobial peptides (AMPs) that act against bacteria, fungi and protozoans. Although these molecules are well-studied among the hylids, AMPs ontogeny remains unknown. Therefore, we performed peptidomic and proteomic analyses of Pithecopus nordestinus (formerly Phyllomedusa nordestina) in order to evaluate the peptide content in post-metamorphosed juveniles and adult individuals. Methods: Cutaneous secretion of both life stages of individuals was obtained and analyzed by LC-MS/MS after reduction and alkylation of disulfide bonds or reduction, alkylation and hydrolysis by trypsin. Results: Differences in the TIC profile of juveniles and adults in both treatments were observed. Moreover, the proteomic data revealed known proteins and peptides, with slight differences in the composition, according to the life stage and the treatment. AMPs were identified, and bradykinin-potentiating peptides were observed in trypsin-treated samples, which suggests a protein source of such peptide (cryptide). Conclusion: In general, skin secretion contents were similar between juveniles and adults, varying in quantity, indicating that the different stages of life are reflected in the number of molecules and not on their diversity.(AU)


Subject(s)
Animals , Female , Peptides , Trypsin , Proteomics , Amphibians , Bodily Secretions , Hydrolysis
6.
Infectio ; 24(4): 229-233, oct.-dic. 2020. tab
Article in Spanish | LILACS, COLNAL | ID: biblio-1114874

ABSTRACT

Resumen Introducción: la fibrosis quística (FQ) es una enfermedad autosómica recesiva que aumenta la viscosidad de las secreciones, en especial las del árbol respiratorio; genera inflamación crónica y colonización/infección por microorganismos, conduciendo a deterioro de la función pulmonar y muerte. Nuestro estudio evaluó la calidad del esputo de pacientes con FQ que ingresaron al Laboratorio de Infectados de la UdeA con base a los criterios de Murray. Metodología: estudio descriptivo con información retrospectiva, incluyendo todos los esputos de pacientes con FQ, recolectados entre enero de 2015 a diciembre de 2018. Resultados: se analizaron 686 muestras de 85 pacientes, de las cuáles se obtuvo cultivo positivo en 501 (73 %) y el 21 % no cumplían los criterios de calidad según Murray. De 908 aislamientos identificados, 823 (90.6 %) corresponden a microorganismos considerados como patógenos en la vía aérea de los pacientes con FQ donde se incluyen S aureus, Pseudomonas spp, H influenzae, Burkhordelia spp, A. xylosoxidans, S maltophilia, A fumigatus, entre otras. Conclusiones: los criterios de Murray no se deben utilizar para definir el procesamiento o no del esputo en pacientes con FQ.


Abstract Introduction: Cystic fibrosis (CF) is an autosomal recessive disease that affects the production and viscosity of secretions, especially the origin of the respiratory airways; generating chronic inflammation and colonization / infection by microorganisms, leading to functional deterioration and death. Our study evaluated the quality of sputum samples from patients with CF who enter the Infected Laboratory of the UDEA based on the Murray criteria. Methodology: A descriptive study with retrospective information was carried out. All sputum from patients with CF were included, collected between January 2015 and December 2018 in the Infected Laboratory of the University of Antioquia. Results: We analyzed 686 samples from 85 patients with CF, positive culture was obtained in 501 (73 %), considering that 21 % of the respiratory samples did not meet the quality criteria according to Murray criteria. Of 908 isolates identified, 823 (90.6 %) correspond to microorganisms considered as pathogens in the airway of CF patients including S aureus, Pseudomonas spp, H influenzae, Burkhordelia spp, A. xylosoxidans, S maltophilia, A fumigatus, among others. Conclusions: The Murray criteria should not be used to define the processing or not of the sputum in patients with CF, all should be processed.


Subject(s)
Sputum , Cystic Fibrosis , Respiratory Tract Infections , Colombia , Bodily Secretions , Infections , Laboratories
8.
Caracas; Observatorio Nacional de Ciencia, Tecnología e Innovación; ago. 2020. 57-78 p. ilus.(Observador del Conocimiento. Revista Especializada de Gestión Social del Conocimiento, 5, 3).
Monography in Spanish | LILACS, LIVECS | ID: biblio-1120131

ABSTRACT

El objetivo principal de este trabajo es describir los principales aspectos biológicos, epidemiológicos y clínicos,con especial énfasis en la fisiopatología, ocasionado por el SARS-CoV-2.El Coronavirus 2 del Síndrome Agudo Respiratorio Severo,es un orthocoronavirinae, del grupoI V, pertenecientes al género betacoronavirus,que causa la actual enfermedad pandémica definida por la Organización Mundial de la Salud (OMS)(2020), como COVID-19.Esta es considerada por los expertos mundiales como una infección viral emergente. Su origenes veterinario y su transmisión zoonótica, a partir de virus que tienen como reservorios a murciélagos y como hospedador intermediario al pangolín, desde el cual se infiere que pudo adaptarse al ser humano, para transmitirse de persona a persona,mediante gotitas respiratorias y secreciones nasales contaminadas con partículas altamente infecciosas. No se descarta la transmisión fecal-oral. La enfermedad posee un periodo de incubación de 2 a 14 días (promedio:5,2 días) y hasta ahora las medidas de prevención y control más eficientes son la cuarentena social obligatoria, el uso de tapabocas, lavado de manos con agua y jabón por al menos 20 segundos,o con productos en gel con alcohol al 70%, mantener una distancia mínima entre las personas de 2 metros. Lo aprendido de los otros dos betacoronavirus humanos relacionados, causantes de las epidemias previas: SARS-CoVen 2002-2003 y el MERSen 2012 ,permiten encarar de mejor forma la actual pandemia(AU)


The main objective of this work is to describe the main biological, epidemiological and clinical aspects, with special emphasis on pathophysiology, caused by SARS-CoV-2. Coronavirus 2 of Severe Acute Respiratory Syndrome is a member of group IV orthocoronavirinae, belonging to the betacoronavirus genus, which causes the current pandemic disease defined by the World Health Organization as COVID-19. This is considered by world experts as an emerging viral infection. Its origin is veterinary and its zoonotic transmission, from viruses that have bats as reservoirs to pangolin an as an intermediate host, from which it is inferred that it could have adapted to the human being, transmitted from person to person by means of respiratory droplets, and highly infectious contaminated nasal secretions Fecal-oral transmission is not ruled out. The disease has an incubation period of 2 to 14 days (5.2 days in mean), and until now the most efficient prevention and control measures are the obligatory social quarantine, the use of face masks, hand washing with soap and water for at least 20 seconds, or with products in gel with 70 alcohol, keeping a minimum distance between people of 2 meters. What has been learned from the other two related human betacoronaviruses that caused the previous epidemics: SARS-CoV in 2002-2003 and MERS in 2012, allow us to better face the current pandemic(AU)


Subject(s)
Humans , Viruses , Hand Disinfection , Causality , Coronavirus Infections/prevention & control , Disease Transmission, Infectious , Bodily Secretions , Severe Acute Respiratory Syndrome , Pandemics
10.
Article in Chinese | WPRIM | ID: wpr-880400

ABSTRACT

According to the actual requirements of pediatric intensive care, a suction detection system of pediatric oral secretions integrated with monitoring function is designed. The system has the function of adjustable intermittent attraction. The duration and proportion of intermittent attraction can be adjusted according to the individualized needs of pediatric intensive care. The suction head of pacifier can reduce the mechanical damage to pediatric oral mucosa as much as possible. Meanwhile, the system can detect and monitor the real-time biochemical indexes of the collected oral secretions, which can be used to help the judgement of aspiration and quantitatively evaluate the microcirculation dysfunction.


Subject(s)
Bodily Secretions , Child , Humans , Mouth , Suction/instrumentation
11.
Rev. cuba. med. mil ; 48(3): e335, jul.-set. 2019. tab, fig
Article in Spanish | LILACS, CUMED | ID: biblio-1126628

ABSTRACT

Introducción: El Acinetobacter spp. se ha convertido en un germen de gran relevancia clínica, resulta un verdadero paradigma de las infecciones nosocomiales multirresistentes. Objetivo: Caracterizar los aislamientos microbiológicos de Acinetobacter spp. en infecciones asociadas a la asistencia sanitaria. Métodos: Estudio descriptivo que incluyó 280 aislamientos de Acinetobacter spp. de las muestras provenientes de pacientes hospitalizados, en el periodo de tres años (del 2016 al 2018) en el Hospital Comandante "Manuel Fajardo Rivero". Las variables del estudio fueron: salas de procedencia del aislamiento, tipo de muestra, factor predictivo, diagnóstico infectológico, susceptibilidad antimicrobiana in vitro y multidrogorresistencia, Resultados: El mayor número de aislamientos de Acinetobacter spp. se obtuvo en la unidad de cuidados intensivos (78,9 por ciento), las secreciones respiratorias fueron las muestras con más aislamientos (58,9 por ciento), la ventilación mecánica resultó el factor predictivo más frecuente (67,9 por ciento) y como diagnóstico infectológico, la neumonía asociada al ventilador (66,8 por ciento). Se encontró un porcentaje elevado de cepas con multidrogorresistencia (73,6 por ciento). Conclusiones: El Acinetobacter spp. se encuentra vinculado a las infecciones asociadas a los servicios de salud, fundamentalmente en los cuidados intensivos. Los antimicrobianos probados evidenciaron altos porcentajes de resistencia, con predominio de las cepas multidrogorresistentes(AU)


Introduction: Acinetobacter spp. has become a germ of great clinical relevance, it is a true paradigm of multiresistant nosocomial infections. Objective: To characterize the microbiological isolates of Acinetobacter spp. in infections associated with health care. Methods: Descriptive study that included 280 isolates of Acinetobacter spp. from the samples of hospitalized patients, in the period of three years (from 2016 to 2018) in the Hospital Comandante "Manuel Fajardo Rivero". The variables of the study were: wards of origin of the isolation, type of sample, predictive factor, infectious diagnosis, antimicrobial susceptibility in vitro and multidrug resistance. Results: The highest number of isolates of Acinetobacter spp. was obtained in the intensive care unit (78.9 percent), the respiratory secretions were the samples with the most isolations (58.9 percent), mechanical ventilation was the most frequent predictor (67.9 percent) and as an infectious diagnosis, ventilator-associated pneumonia (66.8 percent). A high percentage of strains with multidrug resistance (73.6 percent) was found. Conclusions: Acinetobacter spp. is linked to infections associated with health services, mainly in intensive care. The antimicrobials tested showed high percentages of resistance, with a predominance of multidrug resistant strains(AU)


Subject(s)
Acinetobacter Infections/parasitology , Acinetobacter Infections/drug therapy , Cross Infection/microbiology , Bodily Secretions , Epidemiology, Descriptive , Observational Study
12.
Neumol. pediátr. (En línea) ; 14(2): 100-104, jul. 2019. ilus
Article in Spanish | LILACS | ID: biblio-1015017

ABSTRACT

Primary ciliary dyskinesia is a rare autosomal recessive disease with compromised mucociliary drainage. Among the most commonly recommended non-pharmacological therapeutic strategies are secretion drainage techniques. However, the evidence for the use and effectiveness of these techniques is low, and they are generally based on extrapolated evidence of cystic fibrosis. This article reviews the recommendations and available evidence of chest physiotherapy, mainly manual and instrumental techniques of bronchial drainage and physical exercise in children with primary ciliary dyskinesia.


La disquinesia ciliar primaria es una enfermedad autosómica recesiva rara con compromiso del drenaje mucociliar. Entre las estrategias terapéuticas no farmacológicas más comúnmente recomendadas se encuentra las técnicas de drenaje de secreciones. Sin embargo, la evidencia del uso y efectividad de estas técnicas es reducida y generalmente se basan en evidencia extrapolada de la fibrosis quística. Este artículo revisa las recomendaciones y la evidencia disponible de la kinesiología respiratoria, principalmente las técnicas manuales e instrumentales de drenaje bronquial y el ejercicio físico en niños con disquinesia ciliar primaria.


Subject(s)
Humans , Infant , Child , Adult , Pneumonia/therapy , Respiratory Therapy/methods , Kartagener Syndrome/diagnosis , Physical Therapy Modalities , Exercise/physiology , Drainage/instrumentation , Bodily Secretions
13.
Rev. biol. trop ; 67(1): 1-10, Jan.-Mar. 2019. graf
Article in English | LILACS | ID: biblio-1041889

ABSTRACT

Abstract Phyllomedusa azurea is a frog species well distributed geographically in South America, including Brazilian biomes as Pantanal and Cerrado. Compared with other anurans from the Phyllomedusinae family, there are few reports on the bioactive potential of skin-derived molecules from this species. In this perspective, the aim of the present study was to evaluate the in vitro antibacterial activity of skin secretion of P. azurea by detection of Minimum Inhibitory Concentration (MIC) of the growth of bacterial indicator strains and to determine if occurs a changing in the bacterial cell envelope permeability. The MIC determination was carried out by the microdilution plate method. The absorbance was measured and analyzed statistically using the t-test to compare two groups (0.05 % of significance). The impact of the crude extract on cell envelope permeability of Staphylococcus aureus ATCC 25923 was conducted by the crystal violet assay, and the absorbance was measured spectrophotometry followed by the calculation of the crystal violet uptake percentage. The specific MIC for S. aureus ATCC 25923 and Escherichia coli ATCC 25922 was 31.25 µg/mL, while for Bacillus subtilis ATCC 6633 was 125 µg/mL and Pseudomonas aeruginosa ATCC 27853 was 250 µg/mL. The treatment with crescent concentrations of frog skin secretion increased the crystal violet uptake by S. aureus ATCC 25923 cells, suggesting an action on the cell plasma membrane. The results demonstrated that the skin secretion of P. azurea presents antibacterial activity and merit further investigations to characterize the bioactive molecules.(AU)


Resumen P. azurea es una especie de rana bien distribuida geográficamente en América del Sur, que incluye biomas brasileños como Pantanal y Cerrado. En comparación con otros anuros de Phyllomedusinae, existen pocos informes sobre el potencial bioactivo de las moléculas derivadas de la piel de esta especie. En esta perspectiva, el objetivo del presente estudio fue evaluar la actividad antibacteriana in vitro de la secreción de la piel de P. azurea mediante la detección de la Concentración Inhibitoria Mínima (CIM) del crecimiento de cepas indicadoras bacterianas y determinar si ocurre un cambio en la permeabilidad de la envoltura celular bacteriana. La determinación de MIC se llevó a cabo mediante el método de la placa de microdilución. La absorbancia se midió y se analizó estadísticamente mediante la prueba t para comparar dos grupos (0.05 de significancia). El impacto del extracto crudo sobre la permeabilidad de la envoltura celular de Staphylococcus aureus ATCC 25923 se realizó mediante el ensayo de cristal violeta, y se midió la absorbancia mediante espectrofotometría seguida del cálculo del porcentaje de absorción de violeta cristal. La CIM específica para S. aureus ATCC 25923 y Escherichia coli ATCC 25922 fue 31.25 μg / ml, mientras que para Bacillus subtilis ATCC 6633 de 125 μg / ml y Pseudomonas aeruginosa ATCC 27853 de 250 μg / ml. El tratamiento con concentraciones crecientes de secreción de piel de rana aumentó la absorción de violeta cristal por las células de S. aureus ATCC 25923, sugiriendo una acción sobre la membrana plasmática de la célula. Los resultados demostraron que la secreción de la piel de P. azurea presenta actividad antibacteriana y amerita más investigaciones para caracterizar las moléculas bioactivas.(AU)


Subject(s)
Anura/microbiology , Microbial Sensitivity Tests/instrumentation , Ecosystem , Bodily Secretions , Brazil
14.
J. bras. pneumol ; 45(3): e20180058, 2019. tab, graf
Article in English | LILACS | ID: biblio-990114

ABSTRACT

ABSTRACT Objective: To investigate the effects of manual chest compression (MCC) on the expiratory flow bias during the positive end-expiratory pressure-zero end-expiratory pressure (PEEP-ZEEP) airway clearance maneuver applied in patients on mechanical ventilation. The flow bias, which influences pulmonary secretion removal, is evaluated by the ratio and difference between the peak expiratory flow (PEF) and the peak inspiratory flow (PIF). Methods: This was a crossover randomized study involving 10 patients. The PEEP-ZEEP maneuver was applied at four time points, one without MCC and the other three with MCC, which were performed by three different respiratory therapists. Respiratory mechanics data were obtained with a specific monitor. Results: The PEEP-ZEEP maneuver without MCC was enough to exceed the threshold that is considered necessary to move secretion toward the glottis (PEF − PIF difference > 33 L/min): a mean PEF − PIF difference of 49.1 ± 9.4 L/min was achieved. The mean PEF/PIF ratio achieved was 3.3 ± 0.7. Using MCC with PEEP-ZEEP increased the mean PEF − PIF difference by 6.7 ± 3.4 L/min. We found a moderate correlation between respiratory therapist hand grip strength and the flow bias generated with MCC. No adverse hemodynamic or respiratory effects were found. Conclusions: The PEEP-ZEEP maneuver, without MCC, resulted in an expiratory flow bias superior to that necessary to facilitate pulmonary secretion removal. Combining MCC with the PEEP-ZEEP maneuver increased the expiratory flow bias, which increases the potential of the maneuver to remove secretions.


RESUMO Objetivo: Avaliar os efeitos da compressão torácica manual (CTM) sobre o flow bias expiratório durante a manobra positive end-expiratory pressure-zero end-expiratory pressure (PEEP-ZEEP) para a remoção de secreção em pacientes sob ventilação mecânica invasiva. O flow bias, que influencia na remoção de secreção pulmonar, foi avaliado pela razão e diferença entre pico de fluxo expiratório (PFE) e pico de fluxo inspiratório (PFI). Métodos: Estudo cruzado e randomizado no qual participaram 10 pacientes. A manobra PEEP-ZEEP foi aplicada em quatro momentos, sendo um sem CTM e os outros três em associação com a CTM, que foram aplicadas por três fisioterapeutas distintos. Um monitor específico foi utilizado para o registro dos dados de mecânica respiratória. Resultados: A manobra PEEP-ZEEP sem a CTM foi suficiente para ultrapassar o limiar do flow bias expiratório (diferença PFE − PFI > 33 l/min), considerado necessário para deslocar a secreção em direção à glote; a média da diferença PFE − PFI encontrada foi de 49,1 ± 9,4 l/min. A média da razão PFE/PFI alcançada foi de 3,3 ± 0,7. A associação da CTM à PEEP-ZEEP aumentou a média da diferença PFE − PFI em 6,7 ± 3,4 l/min. Foi observada correlação moderada entre a força de preensão manual dos fisioterapeutas e o flow bias gerado durante a CTM. Não foram encontradas alterações hemodinâmicas ou respiratórias adversas ao longo do estudo. Conclusões: A manobra PEEP-ZEEP sem a CTM resultou em um flow bias expiratório superior ao considerado efetivo para auxiliar na remoção de secreção pulmonar. A associação com a CTM aumentou o flow bias expiratório, o que aumenta o potencial da manobra para remover secreções.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Young Adult , Respiration, Artificial/methods , Positive-Pressure Respiration/methods , Pulmonary Ventilation/physiology , Thoracic Wall/physiopathology , Lung/physiology , Reference Values , Respiration, Artificial/adverse effects , Time Factors , Linear Models , Respiratory Mechanics/physiology , Analysis of Variance , Treatment Outcome , Cross-Over Studies , Bodily Secretions , Arterial Pressure/physiology
15.
Braz. J. Pharm. Sci. (Online) ; 54(1): e17261, 2018. tab, graf
Article in English | LILACS | ID: biblio-951916

ABSTRACT

ABSTRACT Equigan is an anabolic steroid that has been developed for veterinary use and derived from endogenous sex hormone testosterone that plays a key role in the development of male reproductive tissue as well as in puberty and spermatogenesis. The current study is aimed to investigate the possible prophylactic effect of star anise extracts (SAE) on the toxicity of rat testes, sexual hormones alternations, sperm count, sperm abnormalities and testicular DNA damage by Equigan. Forty adult male rats were equally divided into four groups (1st Control group, 2nd SAE group, 3rd Equigan and 4th Equigan+SAE group). Food and fluid intakes, relative body weight, potassium, chloride, phosphorous, non-progressive and immotile sperms were significantly increased in Equigan group as compared to control group. In contrast; relative testes weight, sodium, magnesium, total calcium, testosterone, FSH, LH, PRL, sperm count, progressive motility, and viability showed a significant decrease in Equigan group as compared to control groups. The relative weight of epididymis, seminal vesicles, prostates and serum calcium ions didn't change significantly in different studied groups. Co-administration of SAE with Equigan improved the sexual toxicity, electrolyte alternations, sperm count, abnormalities and DNA damage induced by Equigan.


Subject(s)
Animals , Male , Rats , Plant Extracts/analysis , Reproductive Techniques , Illicium/adverse effects , Reproductive Physiological Phenomena , Spermatogenesis/drug effects , Bodily Secretions , DNA Fragmentation/drug effects , Fertility Agents, Male/analysis , Anabolic Agents/pharmacology
16.
J. venom. anim. toxins incl. trop. dis ; 24: 36, 2018. tab, graf, ilus
Article in English | ID: biblio-976026

ABSTRACT

Animal poisons and venoms are sources of biomolecules naturally selected. Rhinella schneideri toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads' secretion has gaining attention. Frog skin is widely known to present great number of host defense peptides and we hypothesize toads present them as well. In this study, we used a RNA-seq analysis from R. schneideri skin and biochemical tests with the gland secretion to unravel its protein molecules. Methods: Total RNA from the toad skin was extracted using TRizol reagent, sequenced in duplicate using Illumina Hiseq2500 in paired end analysis. The raw reads were trimmed and de novo assembled using Trinity. The resulting sequences were submitted to functional annotation against non-redundant NCBI database and Database of Anuran Defense Peptide. Furthermore, we performed caseinolytic activity test to assess the presence of serine and metalloproteases in skin secretion and it was fractionated by fast liquid protein chromatography using a reverse-phase column. The fractions were partially sequenced by Edman's degradation. Results: We were able to identify several classes of antimicrobial peptides, such as buforins, peroniins and brevinins, as well as PLA2, lectins and galectins, combining protein sequencing and RNA-seq analysis for the first time. In addition, we could isolate a PLA2 from the skin secretion and infer the presence of serine proteases in cutaneous secretion. Conclusions: We identified novel toxins and proteins from R. schneideri mucous glands. Besides, this is a pioneer study that presented the in depth characterization of protein molecules richness from this toad secretion. The results obtained herein showed evidence of novel AMP and enzymes that need to be further explored.(AU)


Subject(s)
Anura/physiology , Poisons , Metalloproteases , Serine Proteases , Bodily Secretions , Sequence Analysis, Protein
17.
Article in English | WPRIM | ID: wpr-716854

ABSTRACT

It is well known that the incidence of youth-onset type 2 diabetes is increasing worldwide. On the other hand, most studies have shown that the majority of youth-onset type 2 diabetes occurs in obese individuals, generally with a body mass index (BMI) greater than the 90th or 95th centile for sex- and age-matched children and adolescents. However, we identified some Japanese children with nonobese type 2 diabetes and BMI less than 90th centile by a urine glucose screening program at schools in the Tokyo Metropolitan Area. According to the results obtained from the screening, clinical characteristics of patients with nonobese type 2 diabetes seemed to be different from those in obese type 2 diabetes. Nonobese patients tended to show lower insulin secretion abilities and milder, but evident, insulin resistance from the time of diagnosis. Female, low birth weight (small for gestational age), and genetic background, not related to β-cell-associated autoimmunity, may play a role in development of nonobese type 2 diabetes. In addition, nonobese patients tend to progress earlier to pharmacological treatment including oral hypoglycemic drugs and insulin. Further studies are needed to confirm to these findings and clarify the pathophysiology of children with nonobese type 2 diabetes.


Subject(s)
Adolescent , Asian Continental Ancestry Group , Autoimmunity , Bodily Secretions , Body Mass Index , Child , Diagnosis , Female , Genetic Background , Glucose , Hand , Humans , Hypoglycemic Agents , Incidence , Infant, Low Birth Weight , Infant, Newborn , Insulin , Insulin Resistance , Mass Screening
18.
Rev. med. Risaralda ; 23(2): 4-9, jul.-dic. 2017. ilus, tab
Article in Spanish | LILACS, COLNAL | ID: biblio-902074

ABSTRACT

El objetivo de la investigación fue evaluar la capacidad de formación de biopelículas en especies del género Candida provenientes de muestras clínicas con procesos infecciosos. La muestra estuvo constituida por 70 aislados obtenidos de laboratorios clínicos privados. Para la identificación de las especies se utilizó la Prueba de tubo germinativo y la técnica de microcultivo en agar harina de maíz con tween 80, mientras que la cuantificación de la capacidad de formación de biopelículas se realizó por la técnica de microplaca de poliestireno. Entre los resultados obtenidos en la investigación las especies aisladas fueron: Candida glabrata (34%), Complejo Candida albicans (30%), Complejo Candida parapsilosis (27%) y Candida tropicalis (9%), de las cuales 41 aislados (59%) formaron biopelículas, resultando el Complejo Candida albicans y Candida glabrata las especies con mayor grado de formación de biopelículas con 29% cada una, seguida de Complejo Candida parapsilosis con 27% y Candida tropicalis con 15%. Las cepas que tuvieron mayor formación de biopelículas provenían de orina, seguidas de secreción ótica, secreción vaginal y sangre, sin embargo al aplicar la prueba de Chi-cuadrado (x2) de Pearson se determinó que no existe asociación estadísticamente significativa entre la capacidad de formación de biopelículas y el grado de formación con la procedencia clínica así como tampoco hay asociación entre la capacidad de formación de biopelículas y grado de formación con la especie involucrada (p > 0,05)


The aim of this research was to evaluate the capability of biofilm formation in Candida genus species from clinical samples with infectious conditions. The sample consisted of 70 isolates obtained from private clinical laboratories. Germ tube test and agar cornmeal with tween 80 microculture technique were used as species identification, meanwhile, quantification of biofilm formation capacity was performed by the polystyrene microplate technique. Among the results obtained in the research, isolated species were: Candida glabrata (34%), Candida albicans Complex (30%), Candidaparapsilosis Complex (27%) and Candida tropicalis (9%), of which 41 isolates (59%) formed biofilms, resulting Candida albicans and Candida glabrata Complex species with the highest degree of biofilms with 29% each, followed by Candida parapsilosis Complex with 27% and Candida tropicalis with 15%. Strains with more biofilm formation came from urine, followed by ear discharge, vaginal secretion and blood. However, by applying the Chi squaretest the Pearson, it was determined that there was no statistically significant association between thecapability of biofilm formation and the formation degree with clinical origin, neither there is association between the ability of biofilm formation and the formation degree with the species involved (p> 0.05).


Subject(s)
Humans , Female , Candida , Candida albicans , Biofilms , Vaginal Discharge , Bodily Secretions , Polysorbates , Polystyrenes , Zea mays , Candida glabrata , Candida tropicalis , Agar , Candida parapsilosis , Laboratories
19.
Rev. méd. hered ; 28(4): 236-241, oct.-dic. 2017. graf
Article in Spanish | LILACS, LIPECS | ID: biblio-991434

ABSTRACT

Objetivos: Determinar la frecuencia de microorganismos y el patrón de susceptibilidad antimicrobiana en aislamientos de cultivos de secreción endotraqueal en la unidad de cuidados intensivos de un hospital nacional de Lima. Material y métodos: Estudio observacional, descriptivo. Se incluyeron 195 cultivos positivos de secreción endotraqueal de pacientes en ventilación mecánica durante el periodo enero a diciembre del 2016. Resultados: Acinetobacter sp. fue la bacteria más frecuentemente aislada (28%) seguido por Pseudomonas aeruginosa (22%) y Klebsiella pneumoniae (14%), Los aislamientos con Acinetobacter sp. presentaron una elevada resistencia a antibióticos carbapenémicos (meropenem 90% eimipenem 88%). Conclusiones: Se identificó una elevada frecuencia de Acinetobacter sp. En cultivos de secreción traqueal en la unidad de cuidados intensivos, con un patrón de multirresistencia. (AU)


Objectives: To determine the prevalence of micro-organisms and their susceptibility pattern among endotracheal isolates in an intensive care unit in national hospital in Lima. Methods: An observational, descriptive study was carried-out from January to December 2016, 195 positive isolates from endotracheal aspirates of patients under mechanical ventilation were analyzed. Results: Acinetobacter spp. was the most common bacteria isolates (28%), followed by Pseudomonas aeruginosa (22%) and Klebsiella pneumoniae (14%). Acinetobacter spp. was highly resistant to carbapenems (90% to meropenem and 88% to imipenem). Conclusions: Multi-drug resistant Acinetobacter spp. was the most common pathogen isolated in this setting. (AU)


Subject(s)
Humans , Trachea , Actinobacteria , Bodily Secretions , Intensive Care Units , Epidemiology, Descriptive , Observational Studies as Topic
20.
Rev. bras. ginecol. obstet ; 39(1): 31-34, Jan. 2017. graf
Article in English | LILACS | ID: biblio-843904

ABSTRACT

ABSTRACT The development of a tubocutaneous fistula due to endometriosis in a post-cesarean section surgical scar is a rare complication that generates significant morbidity in the affected women. Surgery is the treatment of choice in these cases. Hormonal therapies may lead to an improvement in symptoms, but do not eradicate such lesions. In this report, we present a 34-year-old patient with a cutaneous fistula in the left iliac fossa with cyclic secretion. Anamnesis, a physical examination, and supplementary tests led us to suggest endometriosis as the main diagnosis, which was confirmed after surgical intervention.


RESUMO O desenvolvimento de fístula tubocutânea secundária à endometriose em cicatriz cirúrgica após cesariana é uma complicação rara, que gera importante morbidade às mulheres acometidas. A cirurgia é o tratamento de escolha nesses casos. Terapias hormonais podem conduzir a uma melhora dos sintomas, mas, de forma alguma, levam à erradicação de tais lesões. No presente relato, temos uma paciente de 34 anos de idade que apresentava uma fístula cutânea em fossa ilíaca esquerda com secreção cíclica. Anamnese, exame físico e exames complementares nos levaram a aventar como principal hipótese diagnóstica a endometriose, que foi confirmada após intervenção cirúrgica.


Subject(s)
Humans , Female , Adult , Cutaneous Fistula/etiology , Endometriosis/complications , Fallopian Tube Diseases/etiology , Fistula/etiology , Genital Diseases, Female/complications , Postoperative Complications/etiology , Bodily Secretions , Cesarean Section , Cicatrix/complications , Cutaneous Fistula/diagnosis , Diagnosis, Differential
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