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1.
São Paulo; s.n; 20240301. 45 p.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1532367

ABSTRACT

A Doença Creutsfeldt- Jacob (DCJ) é caracterizada por alterações neurodegenerativas através do acúmulo da proteína príon codificada (PrPC), nos órgãos linfoides, músculos esqueléticos e no SNC, sua forma vDCJ é a única que apresenta evidências de transmissão dos príons a partir da ingestão de alimentos de origem bovina contaminados. Há possibilidade de transmissão de príons por meio da utilização de enxertos ósseos bovinos em pacientes que necessitam de reconstrução e aumento de dimensão óssea resultantes de perdas dentárias e procedimentos reabilitadores da cavidade oral com implantes dentários, sendo esses pacientes aptos pelos bancos de sangue a doar sangue, surgindo o risco de transmissão, o que propagaria a disseminação da doença. Esta revisão teve como objetivo avaliar o risco de contaminação por príons e o desenvolvimento da doença de Creutzfeldt-Jakob ao uso de enxerto ósseo xenógeno de origem bovina, utilizado em procedimento cirúrgico odontológico. As bases de dados Pubmed, Web of Science, Scopus, LILACS, EMBASE, Google Scholar e Open grey foram pesquisadas almejando trabalhos publicados na língua inglesa, até março de 2021. Dos 2518 artigos potencialmente relevantes, 6 foram selecionados, porém nenhum incluído para o trabalho final, pois avaliaram outras formas de abordagem, riscos através do processo de esterilização e uso de instrumentais contaminados. Portanto, conclui-se que atualmente não há evidências científicas na literatura sobre o risco de contaminação do uso de osso bovino em reabilitações odontológicas, existindo apenas uma plausibilidade biológica.


Subject(s)
Bone and Bones
2.
J. afr. imag. méd ; 16(1): 28-32, 2024. figures, tables
Article in French | AIM | ID: biblio-1555119

ABSTRACT

L'IRM corps entier de diffusion 1,5T est une technique d'imagerie qui fournit des renseignements aussi bien morphologique que fonctionnel avec de nombreuses indications notamment dans les pathologies cancéreuses. Objectif: Faire le bilan d'activité de l'IRM corps entier en insistant sur son apport dans la pathologie cancéreuse. Méthodologie:Etude transversale descriptive avec collecte rétrospective des donnéesincluant les patients ayantréalisé une IRM corps entier pourtumeur maligne sur une période de quatre ans. La saisie et l'analyse des données ont été réalisées grâce aux logiciels Epi Info.Résultats:31,19% des patients avaient un âge compris entre 61 et 70 ans. Le cancer de la prostate était le plus représenté avec 34,68% suivi du cancer du sein 31,45%. L'IRM corps entier était réalisée pour le bilan d'extension dans 39,52% et dans 37,10% pour un bilan d'évolutivité. Les localisations métastatiques étaient observées dans 49% des cas avec une atteinte prépondérantede l'étage abdomino-pelvien 72,13%. Les localisations métastatiques osseuses étaient de 73,77%,hépatiques 26,23% et pulmonaire 14,75%. Les atteintes ganglionnaires de 57,14%. Une progression tumorale a été observée chez 53,33% des patients lors du suivi.Conclusion: L'IRM corps entier permet une fine analyse dans l'évaluation de la pathologie cancéreuse pour l'extension tumorale, la réponse thérapeutique et la recherche de cancer primiti


1.5T whole-body diffusion MRI is imaging that provides both morphological and functional information.It presents several indicationsparticularly in oncology. Objective: Assess the activity of whole-body MRI, emphasizing its contribution to oncologyMethodology:Descriptive retrospective study of patients who had undergone whole-body MRIfor malignancy over a four-year period. Data entry and analysis were performed using Epi Info software.Results:31.19% of patients were between 61 and 70 years old. Prostate cancer was the most represented with 34.68% followed by breast cancer 31.45%.Whole-body MRI was requested for an extension assessment in 39.52% and 37.10% for progress assessment. It revealed metastatic abnormalities in 49%. Abdomino-pelvic region was the most affected area with 72.13%. Metastatic organ involvement was predominantly in the bone 73.77%, followed by the liver 26.23% and the lung14.75%. Lymph node involvement was 57.14%.Tumor progression was observed in 53.33% of patients during follow-up.Conclusion:Whole body MRI allows a fine analysis in the evaluation of the cancerous pathology for the tumor extension, the therapeutic response and the search for primary cancer


Subject(s)
Humans , Male , Female , Bone and Bones
3.
J. afr. imag. méd ; 16(1): 22-27, 2024. figures, tables
Article in French | AIM | ID: biblio-1555120

ABSTRACT

Objectifs. Déterminerla place de la scintigraphie osseuse 3 temps dans le diagnostic del'ostéonécrose avasculaire de la tête fémorale (OATF).Matérielset Méthode. Nousavons colligérétrospectivement62 scintigraphies osseuses (SO+)mettant en évidence uneOATF. Ces SO+ ont été réalisées au Service de Médecine Nucléaire du CHU YOde Ouagadougou,de 2017à 2022. Un formulaire de recueil de données a permis de collecter les données générales et les résultats de la scintigraphie osseuse. L'étude descriptive consistait à calculer les fréquences pour les variables qualitatives et les moyennes pour les variables quantitatives.La SO en trois temps a été faite chez tous les patients après injection de 296 à 925 MBq d'un dérivé diphosphonate : l'hydroxyméthyléne diphosphonate(HMDP) marqué au technétium 99 m (Tc99m). Résultats. 62patients ont présenté une SO+ à la recherche scintigraphique de l'OATF de 2016 à 2021. Leur âge moyen est de 35 ± 14,18 ans avec une nette prédominance féminine (75,8%). La principale étiologie retrouvée etaitla drépanocytose. La douleur coxale avec ou sans boiterie a indiquéla réalisation de la SO dans 41,66 % des cas. Lesvingt-cinqpatients (40,3%) qui avaient bénéficié d'une TDM de la hanche concernée ont été adressés pour confirmation du diagnostic (n=4), bilan pré opératoire (n=9), recherche d'autres foyers(n=12). Pour lesonzepatients atteints de pathologie cancéreuse,le diagnostic d'OATF était fortuit au décours d'une SO réalisée dans le cadre de leur bilan d'extension. Sur les images scintigraphiques tardives, les atteintes de type II de GOLLSHALK étaient majoritaires,suivies des atteintes de type III puis de type I. Il n'y avait pas d'atteinte de type IV. Conclusion. La SO est efficace dans la caractérisation de l'OATF avant l'apparition de la symptomatologie clinique et de la traduction radiologique. Lorsque l'IRM est disponible et n'est pas contre indiquée, la SO est la modalité diagnostique à utiliser en deuxième intention.


Objective: Determine the role of three-step bone scintigraphy (BS) in the diagnosis of aseptic osteonecrosis of the femoral head (AOFH).Materials and Method: Retrospectively we collected 62 positive bone scans (BS+) at aseptic osteonecrosis of the femoral (AOFH). These BS+ were carried out at the Nuclear Medicine Department of CHU YO, from 2017 to 2022. A data collection form has been prepared. It made it possible to collect general data and the results of the bone scintigraphy. The descriptive study consisted of calculating the frequencies for the qualitative and the the means for the quantitative variables. The three-step BS was performed in all patients after injection of 296 to 925 MBq of a diphosphonate derivative: hydroxy methylene diphosphonate (HMDP) labeled with technetium 99 m (Tc99m).Results: 62 patients were positive (BS +) in the aseptic avascular osteonecrosis of the femoral head scintigraphic research from 2016 to2021. Their average age is 35 ± 14.18 years with a clear female predominance (75.8%). The main etiology fremains sickle cell anemia. Hip pain with or without lameness indicated the realization of SO in 41.66% of cases. The twenty-five patients (40.3%) who had undergone CT of the affected hip were referred for confirmation of the diagnosis (n=4), preoperative workup (n=9), search for other foci (n=12). For the eleven patients with cancerous pathology, the diagnosis of AOFH was fortuitous after an BS performes as part of their extension workup. On the late scintigraphic images, type II involvement of GOLLSHALK was predominant, followed by by type then type I. There was no type IV involvement. Conclusion: BS is sensitive in the detection of aseptic avascular osteonecrosis of the femoral head before the onset of clinical symptoms and radiological translation. When MRI is available and not contraindicated, BS is the second-line modality


Subject(s)
Humans , Male , Female , Osteonecrosis , Bone and Bones
4.
Braz. j. biol ; 84: e251970, 2024. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1345559

ABSTRACT

Abstract In order to better understand the ossification processes in anurans our study was carried out on tadpoles and adults of Lithobates catesbeianus. In this sense, we characterized the kinetic properties of alkaline phosphatase with p-nitrophenylphosphatase (pNPP) and pyrophosphate (PPi) and evaluated the activities of tartrate-resistant acid phosphatase and acid phosphatase. The enzyme extracts were obtained from tadpoles and adult femurs, which were divided into epiphysis and diaphysis. After homogenization, the samples were submitted to differential centrifugation to obtain cell membranes and, further, to phospholipase C (PIPLC) treatment, to remove membrane-bound proteins anchored by phosphatidylinositol. The average of specific activity for pNPP hydrolysis (at pH 10.5) by alkaline phosphatase released by phosphatidylinositol-specific phospholipase C (PIPLC) from Bacillus cereus among different bone regions at different animal ages was 1,142.57 U.mg-1, while for PPi hydrolysis (at pH 8.0), it was 1,433.82 U.mg-1. Among the compounds tested for enzymatic activity, the one that influenced the most was EDTA, with approximately 67% of inhibition for pNPPase activity and 77% for PPase activity. In the case of kinetic parameters, the enzyme showed a "Michaelian" behavior for pNPP and PPi hydrolysis. The Km value was around 0.6mM for pNPPase activity and ranged from 0.01 to 0.11mM for PPase activity, indicating that the enzyme has a higher affinity for this substrate. The study of pNPP and PPi hydrolysis by the enzyme revealed that the optimum pH of actuation for pNPP was 10.5, while for PPi, which is considered the true substrate of alkaline phosphatase, was 8.0, close to the physiological value. The results show that regardless of the ossification type that occurs, the same enzyme or isoenzymes act on the different bone regions and different life stages of anurans. The similarity of the results of studies with other vertebrates shows that anurans can be considered excellent animal models for the study of biological calcification.


Resumo Para melhor compreender o processo de ossificação em anuros, nosso estudo foi conduzido em girinos e adultos de Lithobates catesbeianus. Nesse sentido, as propriedades cinéticas da fosfatase alcalina com p-nitrofenilfosfato (pNPP) e pirofosfato (PPi) foram caracterizadas, e as atividades enzimáticas das fosfatases ácida e ácida tartarato resistente foram avaliadas. Os extratos enzimáticos foram obtidos de fêmur de girinos e adultos, divididos em epífise e diáfise. Após a homogeneização as amostras foram submetidas à centrifugação diferencial para obter membrana celular e, em seguida, ao tratamento com fosfolipase C (PIPLC), para remover as proteínas de membrana ancoradas por fosfatidilinositol. A média da atividade específica da fosfatase alcalina, liberada pela PIPLC de Bacillus cereus, para a hidrólise de pNPP (pH 10,5) nas diferentes regiões do fêmur e idades dos animais foi de 1.142,57 U.mg-1, enquanto para a hidrólise do PPi (pH 8,0) foi de 1.433,82 U.mg-1. Entre os compostos testados para a atividade enzimática, o de maior influência foi o EDTA, inibindo aproximadamente 67% e 77% das atividades de pNPPase e PPase, respectivamente. Quanto aos parâmetros cinéticos, a enzima apresentou comportamento Michaeliano para a hidrólise dos dois substratos. O valor de Km foi de 0,6 mM para a atividade de pNPPase e variou de 0,01 a 0,11 para a atividade de PPase, indicando uma maior afinidade por esse substrato. O estudo da hidrólise de pNPP e PPi revelou que o pH ótimo aparente de atuação foi de 10,5 para o pNPP e 8,0 para o PPi, próximo ao fisiológico, sendo que esse é considerado o substrato natural da fosfatase alcalina. Os resultados demonstram que, apesar do tipo de ossificação que ocorre, a mesma enzima ou isoenzimas, atuam nos diferentes locais do osso e estágios de vida dos anuros. A similaridade dos estudos com os realizados com outros vertebrados apontam que os anuros podem ser considerados excelentes modelos animais para o estudo da calcificação biológica.


Subject(s)
Animals , Osteogenesis , Alkaline Phosphatase/metabolism , Rana catesbeiana , Bone and Bones/metabolism , Kinetics
5.
Rev Enferm UFPI ; 12(1): e4010, 2023-12-12. tab e graf
Article in English, Portuguese | LILACS, BDENF | ID: biblio-1523661

ABSTRACT

Objetivo: Caracterizar as metástases ósseas e os eventos relacionados ao esqueleto associando-os ao tempo de sobrevida em pessoas submetidas a radioterapia. Métodos Trata-se de um estudo retrospectivo, descritivo e transversal. Foi realizado em um Centro de Alta Complexidade em Oncologia, com prontuários de pessoas diagnosticadas com metástases ósseas. Resultados: Foram analisados 122 prontuários, a maioria dos pacientes era do sexo feminino (59,02%), com média de idade de 58,86 anos (±14,54). Os sítios primários mais incidentes foram a mama (43,44%) e a próstata (30,33%). O número de osso ou grupo de ossos atingidos foi, em média, de 3,09 (±2,14). A coluna vertebral foi a mais acometida (80,33%). Esta região também foi a mais irradiada no tratamento radioterápico (55,74%). A maioria dos pacientes recebeu 10 sessões de radioterapia (70,49%), em média 9,05 (±2,89). Quanto aos eventos relacionados ao esqueleto, 100% dos pacientes apresentavam registros de dor óssea, 32,78% sofreram fratura patológica, com tempo de sobrevida de 32,53 meses, e 10,65% apresentaram compressão medular, com sobrevida média de 41,52 meses. Conclusão: As metástases ósseas foram mais comuns na coluna vertebral. Todos os pacientes apresentaram registros de dor óssea, e a fratura patológica foi associada à menor sobrevida dos indivíduos. Descritores: Metástase Neoplásica; Osso e Ossos; Radioterapia; Enfermagem.


Objective: To characterize bone metastases and skeleton-related events associating them with survival time in people subjected to radiotherapy. Methods:This is a retrospective, descriptive and cross-sectional study. It was conducted in a High-Complexity Oncology Center with medical records of people diagnosed with bone metastases. Results:A total of 122 medical records were analyzed and most of the patients were female (59.02%), with a mean age of 58.86 years old (±14.54). The most incident primary sites were breast (43.44%) and prostate (30.33%). The mean number of bones or bone groups affected was 3.09 (±2.14). The spine was the most affected area (80.33%). This region was also the most irradiated in the radiotherapy treatments (55.74%). Most of the patients (70.49%) underwent 10 radiotherapy sessions, with a mean of 9.05 (±2.89). With regard to the skeleton-related events, 100% of the patients reported pain, 32.78% suffered pathological fractures with a survival time of 32.53 months, and 10.65% presented spine compression with a mean survival of 41.52 months. Conclusion:Bone metastases were more common in the spine. All the patients presented bone painrecords, and pathological fractures were associated with lower survival among the individuals. Descriptors: Neoplasm Metastasis; Bone and Bones; Radiotherapy; Nursing.


Subject(s)
Radiotherapy , Bone and Bones , Nursing , Neoplasm Metastasis
6.
Odovtos (En línea) ; 25(3): 43-54, Sep.-Dec. 2023. tab, graf
Article in English | LILACS, SaludCR | ID: biblio-1529068

ABSTRACT

Abstract The aim of this experimental study was to determine the effect of photobiomodulation therapy on bone repair in a rat tibia osteotomy model at 15 and 30 days. The sample consisted of 36 male Holtzman rats that were randomized into 6 equal groups. Groups A1 and A2: osteotomy + 1 J laser energy. Groups B1 and B2: osteotomy + 3 J laser energy. Groups C1 and C2 (controls): osteotomy only. The bone repair was analyzed by histological evaluation of osteoblasts and osteocytes both at 15 days (groups A1, B1, and C1) and at 30 days (groups A2, B2, and C2). Within the results, in all groups a greater number of osteoblasts was found at 15 days vs 30 days (p<0.05), and a greater number of osteocytes in B1 and C2 vs B2 and C1, respectively (p<0.05). When evaluating the 3 groups worked up to 15 days, more osteoblasts were found in A1 and C1 vs B1 (p<0.001); and osteocytes predominated in A1 and B1 vs C1 (p<0.001). At 30 days there was a greater quantity of osteoblasts in C2 vs A2 and B2 (p<0.05) and of osteocytes in C2 vs B2 (p<0.05). It is concluded that 1 J photobiomodulation therapy improved bone repair at 15 days; however, this improvement was not observed at 30 days because there were no differences between the irradiated groups and the control.


Resumen El objetivo de este estudio experimental fue determinar el efecto de terapia de fotobiomodulación sobre la reparación ósea en un modelo de osteotomía de tibia de rata a los 15 y 30 días. La muestra estuvo compuesta por 36 ratas Holtzman macho que se aleatorizaron en 6 grupos iguales. Grupos A1 y A2: osteotomía + energía láser de 1 Joule. Grupos B1 y B2: osteotomía + energía láser 3 Joule. Grupos C1 y C2 (controles): solo osteotomía. La reparación ósea fue analizada por evaluación histológica de osteoblastos y osteocitos tanto a los 15 días (grupos A1, B1 y C1) como a los 30 días (grupos A2, B2 y C2). Como resultados se encontró que en todos los grupos hubo mayor número de osteoblastos a los 15 días vs. 30 días (p<0,05), y mayor número de osteocitos en B1 y C2 vs B2 y C1, respectivamente (p<0,05). Al evaluar a los animales a los 15 días, se observó mayor número de osteoblastos en A1 y C1 vs B1 (p<0.001); y mayor número de osteocitos en A1 y B1 vs C1 (p<0,001). Al evaluar a los ratones a los 30 días hubo mayor cantidad de osteoblastos en C2 vs A2 y B2 (p<0,05) y de osteocitos en C2 vs B2 (p<0,05). Se concluye que la terapia de fotobiomodulación con 1 Joule mejoró la reparación ósea a los 15 días; sin embargo, dicha mejora no se observó a los 30 días porque no hubo diferencias entre los grupos irradiados y el control.


Subject(s)
Animals , Rats , Tibia , Photobiology , Low-Level Light Therapy , Bone and Bones
7.
Int. j. morphol ; 41(5): 1281-1287, oct. 2023. ilus, tab
Article in English | LILACS | ID: biblio-1521045

ABSTRACT

SUMMARY: Osteotechnics is one of the different anatomical preservation techniques and can be defined as the technique designed to prepare, clean, obtain and preserve bone structures that can be used in the teaching, museographic or research field. The osteotechnical technique procedure consists of the following phases: debulk and disjoint, maceration, cooking, cleaning, degreasing, bleaching, and labeling to obtain bone material. Seven phases will be explained in detail, as well as the materials, instruments, quantities of the substances used, and the time required to obtain human bone material. We consider that this article can serve as a guide, given that all the experimentation was carried out with human biological material. This methodological proposal could be consolidated and established based on the experience acquired during the creation of the contemporary skeletal collection of the department of innovation in human biological material (DIMBIH). Therefore, the purpose of our proposal is to provide tools that facilitate the work of those who carry out this work and fundamentally to avoid irreversible or irreparable damage to the osteological material, since it is of great value and difficult to acquire for disciplines as anatomy, veterinary, physical and forensic anthropology, medicine, dentistry and biology.


La osteotecnia es una de las técnicas diferentes de conservación anatómica y puede definirse como la técnica destinada a preparar, limpiar, obtener y conservar estructuras óseas que pueden ser utilizadas en el ámbito docente, museográfico o de investigación. El procedimiento de la técnica osteotécnica consta de las siguientes fases: descarnado y desarticulado, maceración, cocción, limpieza, desengrase, blanqueo y marcaje para la obtención de material óseo. Se explicarán en detalle siete fases, así como los materiales, instrumentos, cantidades de las sustancias utilizadas y el tiempo necesario para obtener material óseo humano. Consideramos que este artículo puede servir de guía, dado que toda la experimentación se realizó con material biológico humano. Esta propuesta metodológica pudo consolidarse y establecerse a partir de la experiencia adquirida durante la creación de la colección esquelética contemporánea del Departamento de Innovación en Material Biológico Humano (DIMBIH). Por lo tanto, el propósito de nuestra propuesta es brindar herramientas que faciliten el trabajo de quienes realizan este trabajo y fundamentalmente evitar daños irreversibles o irreparables en el material osteológico, ya que es de gran valor y difícil adquisición para las disciplinas como la anatomía, veterinaria, antropología física y forense, medicina, odontología y biología.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Preservation, Biological/methods , Bone and Bones , Anatomy/methods , Anthropology, Physical , Osteology
8.
Rev. Ciênc. Méd. Biol. (Impr.) ; 22(2): 188-196, set 2023. fig
Article in English | LILACS | ID: biblio-1516244

ABSTRACT

Introduction: Hansen's disease, or leprosy is caused by Mycobacterium leprae (M. leprae), is a major public health problem in developing countries, and affecting the skin and peripheral nerves. However, M. leprae can also affect bone tissue, mucous membranes, liver, eyes, and testicles, producing a variety of clinical phenotypes. MicroRNAs (miRNAs) have been expressed in the various clinical forms of leprosy and could potentially be used for its diagnosis. Objective: in silico design of the molecular structure of miRNAs expressed in leprosy. Methodology: we performed a nucleotide sequence search of 17 miRNAs expressed in leprosy, designing in silico the molecular structure of the following miRNAs: miRNA-26a, miRNA-27a, miRNA-27b, miRNA-29c, miRNA-34c, miRNA-92a-1, miRNA- 99a-2, miRNA-101-1, miRNA-101-2, miRNA-125b-1, miRNA-196b, miRNA-425-5p, miRNA-452, miRNA-455, miRNA-502, miRNA-539, and miRNA-660. We extracted the nucleotides were from the GenBank of National Center for Biotechnology Information genetic sequence database. We aligned the extracted sequences with the RNA Folding Form, and the three-dimensional molecular structure design was performed with the RNAComposer. Results: we demonstrate the nucleotide sequences, and molecular structure projection of miRNAs expressed in leprosy, and produces a tutorial on the molecular model of the 17 miRNAs expressed in leprosy through in silico projection processing of their molecular structures. Conclusion: we demonstrate in silico design of selected molecular structures of 17 miRNAs expressed in leprosy through computational biology.


Introdução: a doença de Hansen, ou hanseníase é causada pelo Mycobacterium leprae (M. leprae), é um grande problema de saúde pública nos países em desenvolvimento e afeta, a pele e os nervos periféricos. Entretanto, o M. leprae também pode comprometer o tecido ósseo, membranas mucosas, fígado, olhos e testículos, produzindo uma variedade de fenótipos clínicos. MicroRNAs (miRNAs) têm sido expressos nas várias formas clínicas da hanseníase e podem ser potencialmente utilizados para seu diagnóstico. Objetivo: objetivou-se com esse experimento modelar computacionalmente a estrutura molecular dos miRNAs expressos na hanseníase. Metodologia: realizou-se como metodologia uma pesquisa das sequências nucleotídicas de 17 miRNAs expressos na hanseníase, desenhando em modelo computacional a estrutura molecular dos seguintes miRNAs: miRNA-26a, miRNA-27a, miRNA-27b, miRNA- 29c, miRNA-34c, miRNA-92a-1, miRNA-99a-2, miRNA-101-1, miRNA-101-2, miRNA-125b-1, miRNA-196b, miRNA-425-5p, miRNA-452, miRNA-455, miRNA-502, miRNA-539, e miRNA-660. Extraiu-se os nucleotídeos do banco de dados do GenBank of National Center for Biotechnology Information . Alinhou-se as sequências extraídas com o RNA Folding Form, e o projeto da estrutura molecular tridimensional foi realizado com o RNAComposer. Resultados: demonstrou-se como resultados as sequências dos nucleotídeos e a projeção da estrutura molecular dos miRNAs expressos na hanseníase, e produzimos um tutorial sobre o modelo molecular dos 17 miRNAs expressos em hanseníase através do processamento de suas estruturas moleculares em projeção computacional. Conclusão: foi demonstrado computacionalmente o projeto de estruturas moleculares selecionadas de 17 miRNAs expressos em hanseníase através da biologia computacional.


Subject(s)
Peripheral Nerves , Skin , Biomarkers , MicroRNAs , Leprosy , Mycobacterium leprae , Testis , Bone and Bones , Eye , Liver , Mucous Membrane
9.
Actual. osteol ; 19(2): 128-143, sept. 2023. ilus, tab
Article in Spanish | LILACS, UNISALUD, BINACIS | ID: biblio-1523882

ABSTRACT

El presente trabajo muestra la obtención de un material a partir de un polímero sintético (TerP) y otro natural, mediante entrecruzamiento físico y su caracterización fisicoquímica y biológica, con el fin de emplearlos para regeneración de tejido óseo. Las membranas fueron obtenidas por la técnica de evaporación del solvente y caracterizadas por espectroscopia FTIR, ensayos de hinchamiento, medidas de ángulo de contacto y microscopia electrónica de barrido (SEM). Se encontró que la compatibilidad entre los polímeros que la constituyen es estable a pH fisiológico y que, al incorporar mayor cantidad del TerP a la matriz, esta se vuelve más hidrofóbica y porosa. Además, teniendo en cuenta la aplicación prevista para dichos materiales, se realizaron estudios de biocompatibilidad y citotoxicidad con células progenitoras de médula ósea (CPMO) y células RAW264.7, respectivamente. Se evaluó la proliferación celular, la producción y liberación de óxido nítrico (NO) al medio de cultivo durante 24 y 48 horas y la expresión de citoquinas proinflamatorias IL-1ß y TNF-α de las células crecidas sobre los biomateriales variando la cantidad del polímero sintético. Se encontró mayor proliferación celular y menor producción de NO sobre las matrices que contienen menos proporción del TerP, además de poseer una mejor biocompatibilidad. Los resultados de este estudio muestran que el terpolímero obtenido y su combinación con un polímero natural es una estrategia muy interesante para obtener un biomaterial con posibles aplicaciones en medicina regenerativa y que podría extenderse a otros sistemas estructuralmente relacionados. (AU)


In the present work, the preparation of a biomaterial from a synthetic terpolymer (TerP) and a natural polymer, physically crosslinked, is shown. In order to evaluate the new material for bone tissue regeneration, physicochemical and biological characterizations were performed. The membranes were obtained by solvent casting and characterized using FTIR spectroscopy, swelling tests, contact angle measurements, and scanning electron microscopy (SEM). It was found that the compatibility between the polymers is stable at physiological pH and the incorporation of a higher amount of TerP into the matrix increases hydrophobicity and porosity.Furthermore, considering the intended application of these materials, studies of biocompatibility and cytotoxicity were conducted with Bone Marrow Progenitor Cells (BMPCs) and RAW264.7 cells, respectively. Cell proliferation, NO production and release into the culture medium for 24 and 48 hours, and proinflammatory cytokine expression of IL-1ß and TNF-α from cells grown on the biomaterials while varying the amount of the synthetic polymer were evaluated. Greater cell proliferation and lower NO production were found on matrices containing a lower proportion of TerP, in addition to better biocompatibility. The results of this study demonstrate that the obtained terpolymer and its combination with a natural polymer is a highly interesting strategy for biomaterial preparation with potential applications in regenerative medicine. This approach could be extended to other structurally related systems. (AU)


Subject(s)
Animals , Rats , Osteogenesis , Polymers/chemistry , Biocompatible Materials/chemical synthesis , Bone and Bones/chemistry , Bone Regeneration , Chitosan/chemistry , Polymers/toxicity , Biocompatible Materials/toxicity , Materials Testing , Cell Differentiation , Chromatography, Gel , Spectroscopy, Fourier Transform Infrared , Cell Culture Techniques , Nuclear Magnetic Resonance, Biomolecular , Chitosan/toxicity
10.
Rev. bras. ortop ; 58(4): 659-661, July-Aug. 2023. graf
Article in English | LILACS | ID: biblio-1521791

ABSTRACT

Abstract Supracondylar apophysis (SA) is a bony prominence that originates from the anteromedial aspect of the distal humerus with a lower projection and which, although usually asymptomatic, due to the relationship with adjacent structures can cause symptoms. We describe the case of a 42-year-old woman with pain complaints radiating from her elbow to her hand, with 6 months of evolution. On objective examination, the patient had a sensory deficit in the median nerve territory and decreased grip strength. Radiographs of the distal humerus were performed, in which a bone spike was visible, and magnetic resonance imaging showed thickening of the median nerve epineurium. Electromyography showed severe axonal demyelination of the median nerve proximal to the elbow. A median nerve compression caused by a SA was diagnosed. The patient underwent surgery and, 1 year after the operation, she had a complete clinical recovery. Supracondylar apophysis is a rare, but possible and treatable cause of high median nerve compression.


Resumo A apófise supracondilar (ASC) é uma proeminência óssea que tem origem na face anteromedial do úmero distal com projeção inferior e que, apesar de habitualmente assintomática, pela relação com as estruturas adjacentes pode causar sintomatologia. Descrevemos o caso de uma mulher de 42 anos, com queixas álgicas irradiadas do cotovelo à mão, com 6 meses de evolução. Ao exame objetivo, a paciente apresentava um déficit sensorial no território do nervo mediano e diminuição da força de preensão. Foram realizadas radiografias do úmero distal nas quais era visível uma espícula óssea, e na ressonância magnética era evidente o espessamento do epineuro do nervo mediano. A eletromiografia apresentou uma desmielinização axonal grave do nervo mediano proximal ao cotovelo. Foi diagnosticada uma compressão do nervo mediano por uma ASC. A paciente foi submetida à cirurgia e 1 ano pós-operatório apresentou recuperação clínica total. A ASC é uma causa rara, mas possível e tratável da compressão alta do nervo mediano.


Subject(s)
Humans , Female , Adult , Bone and Bones/surgery , Median Neuropathy , Humerus/surgery
11.
Biomédica (Bogotá) ; 43(2): 157-163, jun. 2023. tab, graf
Article in English | LILACS | ID: biblio-1533931

ABSTRACT

We documented two stages of bone involvement due to syphilis in two adult patients infected with human immunodeficiency virus. Bony lesions of secondary versus tertiary syphilis cannot be differentiated on clinical or radiologic grounds alone. Given the rarity of this clinical presentation, there is no consensus on treatment duration and related outcomes.


Se describen dos etapas de compromiso óseo por sífilis en dos pacientes adultos infectados por el virus de la inmunodeficiencia humana. Las lesiones óseas de la sífilis secundaria y de la sífilis terciaria no se pueden diferenciar únicamente por características clínicas o radiológicas. Dada la rareza de esta presentación clínica, no hay consenso sobre la duración del tratamiento y los resultados relacionados.


Subject(s)
Bone and Bones , Syphilis , Bone Neoplasms , HIV , Neurosyphilis
12.
Int. j. morphol ; 41(3): 894-900, jun. 2023. ilus, tab
Article in English | LILACS | ID: biblio-1514321

ABSTRACT

SUMMARY: It is known that diabetes mellitus has late complications, including microvascular and macrovascular diseases. Diabetes can affect bones through biochemical markers of bone structure, density, and turnover. This study aimed to biomechanically investigate the bone-protective effects of angiotensin 1-7 (Ang 1-7), one of the active peptides in the renin-angiotensin system, in rats with diabetes. Thirty male Wistar albino rats, three months old and weighing 250-300 g, were divided into four groups: diabetes, Ang 1- 7, diabetes plus Ang 1-7, and control. One month later, diabetes developed in rats; the rats were sacrificed, and their right femur was removed. Three-point bending biomechanical tests were performed on the femurs. The diabetic group had significantly higher bone fragility than the other groups (Pr >.05). Bone fragility was lower, and bone flexibility was higher in the Ang 1-7 groups (Pr>F value 0.05). As a result of our study, the effect of Ang 1-7 on the bones of rats with diabetes was investigated biomechanically. Ang 1-7 has a protective impact on the bones of rats with diabetes.


Se sabe que la diabetes mellitus tiene complicaciones tardías, incluyendo enfermedades microvasculares y macrovasculares. La diabetes puede afectar los huesos a través de los marcadores bioquímicos de la estructura, la densidad y el recambio óseo. Este estudio tuvo como objetivo investigar biomecánicamente los efectos protectores en los huesos de la angiotensina 1-7 (Ang 1-7), uno de los péptidos activos en el sistema renina-angiotensina, en ratas con diabetes. Treinta ratas albinas Wistar macho, de tres meses de edad y con un peso de 250-300 g, se dividieron en cuatro grupos: diabetes, Ang 1-7, diabetes más Ang 1-7 y control. Un mes después, se desarrolló diabetes en ratas; se sacrificaron los animales y se extrajo su fémur derecho. Se realizaron pruebas biomecánicas de flexión de tres puntos en los fémures. El grupo diabéticos tenía una fragilidad ósea significativamente mayor que los otros grupos (Pr > 0,05). La fragilidad ósea fue menor y la flexibilidad ósea fue mayor en los grupos Ang 1-7 (valor Pr>F 0,05). Como resultado de nuestro estudio, se determinó biomecánicamente el efecto de Ang 1-7 en los huesos de ratas con diabetes. Se concluye que Ang 1-7 tiene un impacto protector en los huesos de ratas diabéticas.


Subject(s)
Animals , Male , Rats , Peptide Fragments/administration & dosage , Renin-Angiotensin System , Angiotensin I/administration & dosage , Diabetes Mellitus, Experimental , Femur/drug effects , Biomechanical Phenomena , Bone and Bones/drug effects , Rats, Wistar , Disease Models, Animal
13.
Rev. Flum. Odontol. (Online) ; 1(60): 127-136, jan.-abr. 2023.
Article in Portuguese | LILACS, BBO | ID: biblio-1411347

ABSTRACT

O defeito ósseo de Stafne foi considerado por muito tempo como um cisto ósseo por se apresentar radiograficamente de forma radiolúcida, circunscrita, com bordas delimitadas e em quase todos os casos unilateral. Hoje em dia já se sabe que é uma depressão e/ou cavidade óssea causada pelo alojamento da glândula submandibular ou parte dela e/ou seus tecidos adjacentes na região posterior da mandíbula, ou na região anterior em casos mais raros pelo alojamento da glândula sublingual. Sendo assim, o objetivo deste trabalho é realizar uma breve revisão da literatura acerca das características mais frequentes e suas variações desta anomalia anatômica. As buscas foram realizas em periódicos de artigos científicos publicado nas bases de dados eletrônicas: PubMed, Scielo e Google Acadêmico, contendo artigos científicos dos últimos dez anos nos idiomas inglês e português. Os dados mostram que o defeito ósseo de stafne tem predileção pelo sexo masculino, é assintomática, pois se trata de uma alteração anatômica, não requer tratamento, portanto cabe ao cirurgião dentista ter conhecimento, uma vez que este é encontrado de forma ocasional em radiografias panorâmicas para investigação de outras circunstancias, e que muitas vezes acaba levando o profissional odontólogo a realizar diagnósticos errôneos ou exames mutiladores como a biopsia que são desnecessários nesta situação pelo fato desta variação anatômica ter características muitos semelhantes aos cistos.


Stafne's bone defect was considered for a long time as a bone cyst because it presents radiographically in a radiolucent, circumscribed form, with delimited borders and, in almost all cases, unilateral. Nowadays it is known that it is a depression and / or bone cavity caused by the accommodation of the submandibular gland or part of it and / or its adjacent tissues in the posterior region of the mandible, or in the anterior region in rarer cases by the accommodation of the sublingual gland. Therefore, the objective of this work is to carry out a brief literature review of the more specific characteristics and their variations of this anatomical anomaly. Searches were performed in journals of scientific articles published in electronic databases: PubMed, Scielo and Academic Google, containing scientific articles from the last ten years in English and Portuguese. The data show that the stafne bone defect has a predilection for males, it is asymptomatic, as it is an anatomical alteration, does not require treatment, therefore it is up to the dental surgeon to be aware, once panoramic to investigate other circumstances, and that many Sometimes it ends up leading the dental professional to perform misdiagnosis or mutilating tests such as a biopsy, which are unnecessary in this situation because this anatomical variation has characteristics that are very similar to cysts.


Subject(s)
Submandibular Gland Diseases , Tissues , Bone and Bones , Radiography, Panoramic , Mandible
14.
Rev. odontol. UNESP (Online) ; 52: e20230027, 2023. tab, ilus
Article in English | LILACS, BBO | ID: biblio-1530299

ABSTRACT

Introdução: O conhecimento da biomecânica de implantes de diâmetro reduzido indica dimensões seguras para uso clínico. Objetivo: O objetivo do presente estudo foi comparar biomecanicamente implantes de diâmetro regular e reduzido para suporte de próteses implantossuportadas unitárias na região anterior da maxila por meio de análise de elementos finitos 3D (3D-FEA). Material e método: Quatro modelos 3D-FEA foram desenvolvidos a partir de recomposição de tomografia computadorizada e dados da literatura: um bloco ósseo na região incisiva lateral superior direita com implante e coroa. M1: 3,75 x 13 mm, M2: 3,75 x 8,5 mm, M3: 2,9 x 13 mm e M4: 2,9 x 8,5 mm. Foi aplicada carga de 178 N nos ângulos 0, 30 e 60 graus em relação ao longo eixo do implante. Foram avaliados mapas de tensão de Von Mises, tensão principal máxima e microdeformação. Resultado: M3 e M4 apresentaram maiores valores de tensão e microdeformação que M1 e M2, principalmente quando foram aplicadas forças inclinadas. Porém, M3 apresentou comportamento biomecânico melhor do que M4. Conclusão: Pode-se concluir que reduzir o diâmetro dos implantes pode prejudicar a biomecânica durante a aplicação de forças, mas a distribuição e intensidade das tensões, bem como os valores de microdeformação podem ser melhorados se o comprimento do implante for aumentado


Introduction: Narrow diameter implants biomechanics knowledge indicates safe dimensions for clinical use. Objective: Purpose of the present study was biomechanically to compare regular and narrow diameter implants to support single implant-supported prosthesis in the anterior region of the maxilla by 3D finite element analysis (3D-FEA). Material and method: Four 3D-FEA models were developed form CT scan recompositing and literature data: a bone block in the right upper lateral incisive region with implant and crown. M1: 3.75 x 13 mm, M2: 3.75 x 8.5 mm, M3: 2.9 x 13 mm and M4: 2.9 x 8.5 mm. It was applied load was of 178 N at 0, 30 and 60 degrees in relation to implant long axis. Von Mises stress, maximum principal stress and microdeformation maps were evaluated. Result: M3 and M4 did show higher tension and higher microdeformation values than M1 and M2, especially when inclined forces were applied. However, M3 presented enhanced biomechanical behavior than M4. Conclusion: It can be concluded that reduce the diameter of the implants can disadvantage to the biomechanics during the application of forces, but the distribution and intensity of the stresses, as well as the micro deformation values can be improved if the length of the implant is increased


Subject(s)
Prostheses and Implants , Stress, Mechanical , Biomechanical Phenomena , Bone and Bones , Dental Implants , Finite Element Analysis , Tomography, X-Ray Computed , Maxilla
15.
Rev. odontol. UNESP (Online) ; 52: e20230039, 2023. tab, ilus
Article in Portuguese | LILACS, BBO | ID: biblio-1530298

ABSTRACT

Introdução: Diferentes metodologias tem sido utilizadas para avaliação histológica da osseointegração, levando a diferentes resultados. Objetivo: O objetivo é comparar diferentes metodologias de análise histomorfométrica da osseointegração de implantes com diferentes superfícies, instalados em tíbias de ratos. Material e método: 24 ratos foram aleatoriamente divididos em 3 grupos (n = 8) para a instalação dos implantes. Esses grupos foram divididos de acordo com o tipo de implante instalado na tíbia: Grupo Hidrofílico - HFL (instalação de implante com superfície modificada por jateamento de óxidos e ataque ácido e mantida em solução de cloreto de sódio), Grupo Hidrofóbico - HFB (instalação de implante com superfície modificada por jateamento de óxidos e ataque ácido) e Grupo Usinado - U (instalação de implante com superfície usinada). No período de 45 dias após os procedimentos cirúrgicos de instalação dos implantes, os animais foram submetidos à eutanásia, e as tíbias foram removidas, sendo realizado o processamento histológico para amostras não descalcificadas. Após a obtenção das lâminas, foi realizada a análise histomorfométrica para avaliar as porcentagens de contato osso-implante (% BIC) e da área óssea entre as espiras (% BBT). As mensurações foram realizadas em duas diferentes regiões: 1) as roscas do implante na região do osso cortical e 2) todas as roscas do implante inseridas no osso (cortical e medular). A análise estatística foi feita por meio de ANOVA One-Way, seguida pelo teste de Tukey para análise inferencial dos dados. Resultado: Quando o BIC e o BBT foram analisados de forma independente nas regiões cortical e trabecular/total, observou-se uma diferença no comportamento histológico dos implantes de acordo com o tratamento de superfície. Os implantes HFL apresentaram BIC (%) trabecular 16,85% maior (p = 0,02) do que os implantes HFB e 26,12% maior (p ≤ 0,0001) do que os implantes usinados. Contudo, a região cortical de todos os grupos apresentou valores de BIC cortical significativamente maiores ao redor dos implantes, independentemente da superfície. Conclusão: Os resultados obtidos demonstraram que a formação óssea peri-implantar foi superior nas análises realizadas apenas em osso cortical, se comparados aos valores obtidos na mensuração total (osso cortical somado ao medular). Também foi possível observar que, dentro da mesma superfície, os valores de BIC (%) foram superiores em osso cortical. Em relação às limitações do estudo, pode-se concluir que diferentes metodologias de análise histométrica da osseointegração ao redor de diferentes superfícies de implantes instalados em tíbias de ratos podem interferir nos resultados de osseointegração, independentemente da superfície analisada.


Introduction: Different methodologies were used for histological evaluation of osseointegration, leading to different results. Objective: The objective is to compare different methodologies for histomorphometric analysis of the osseointegration of implants with different surfaces, installed in rat tibias. Material and method: Twenty-four rats were randomly divided into 3 groups (n=8) for implant installation. These groups were divided according to the type of implant that will be installed in the tibia: Hydrophilic Group (installation of an implant with a surface modified by oxide blasting and acid attack and maintained in a sodium chloride solution), Hydrophobic Group (installation of an implant with surface modified by oxide blasting and acid attack) and Machined Group (implant installation with machined surface). Within 45 days after the surgical procedures to install the implants, the animals were euthanized and the tibias were removed, and histological processing was carried out for non-decalcified samples. After obtaining the slides, histomorphometric analysis was performed to evaluate the percentages of bone-implant contact (%BIC) and the bone area between the turns (%BBT). Measurements were carried out in two different regions: 1) the implant threads in the cortical bone region and 2) all implant threads inserted into the bone (cortical and medullary). Statistical analysis was performed using One-Way ANOVA followed by Tukey's test for inferential data analysis. Result: The BIC and BBT were analyzed independently in the cortical and trabecular/total regions, a difference was observed in the histological behavior of the implants according to the surface treatment. HFL implants showed trabecular BIC (%) 16.85% higher (p=0.02) than HFB implants and 26.12% higher (p≤0.0001) than machined implants. However, the cortical region of all groups showed significantly higher cortical BIC values around the implants, regardless of the surface. Conclusion: the results obtained demonstrated that peri-implant bone formation was superior in analyzes carried out only on cortical bone, compared to the values obtained in the total measurement (cortical bone plus medullary bone). It was also possible to observe that, even within the same surface, the BIC(%) values were higher in cortical bone. Within the limitations of the study, it can be concluded that different methodologies for histometric analysis of osseointegration around different surfaces of implants installed in rat tibias can interfere with the results of osseointegration regardless of the surface analyzed


Subject(s)
Rats , Tibia , Bone and Bones , Dental Implants , Data Interpretation, Statistical , Osseointegration , Mouth, Edentulous , Analysis of Variance
16.
Article in English | LILACS, CUMED | ID: biblio-1536302

ABSTRACT

Introduction: Tapered implants have shown that thanks to their macro design they are capable of expanding the surgical bed performed by the surgeon, which in clinical practice gives a feeling of greater stability, however it is highly subjective and dependent on the operator. Aim: To analyze the influence of the implant macro design in the primary and secondary stability by means of analysis of resonance frequency and force of insertion. Methods: 38 Screw Type and Tapered Type implants were placed in 18 patients in the Bucomaxillofacial Implantology program of the University of Chile during 2006 and 2007 in type II or III bone jaws according to Leckholm and Zarb. Implant stability, implant stability coefficient (ISQ), was measured through Ostell® mentor at the time of installation (ISQ1) and then at connection (ISQ2) and the Insertion Torque through the Osseoset® machine: 17 Screw Type implants with an approximate average contact area of 237 mm2 (3.75/15 mm; 3.75/13 mm) and 17 Tapered Type implants with an approximate average contact area of 226 mm2 (4.3/13 mm; 4.3/16 mm). Results: The averages of ISQ1 and ISQ2 and Insertion Torque respectively for implants with an approximate contact area of 237 mm2 were 71.3 ISQ1, 66.6 ISQ2 and 44.52 Ncm; for 226 mm2 implants it was 75 ISQ1, 72.5 ISQ2 and 48.82 Ncm. Conclusion: Implants with an average contact area of approximately 226 mm2 (Tapered Type) present significantly higher primary and secondary stability than those with an average contact area of approximately 237 mm2 (Screw Type). (Average ISQ1: p = 0.0473; Insertion Torque: p = 0.0031 and Average ISQ2: p = 0.0039)(AU)


Introducción: Los implantes Cónicos han demostrado que gracias a su macro diseño son capaces de expandir el lecho quirúrgico realizado por el cirujano, lo que en la práctica clínica da una sensación de mayor estabilidad, sin embargo, ella es altamente subjetiva y dependiente del operador. Objetivo: Analizar la influencia del diseño del implante en la estabilidad primaria y secundaria mediante análisis de frecuencia de resonancia y Torque de Inserción. Métodos: 38 implantes Tipo Tornillo y Tipo Cónico fueron colocados en 18 pacientes en el programa de Implantología Bucomáxilofacial de la Universidad de Chile durante el año 2006 y 2007 en maxilares de hueso tipo II o III según Leckholm y Zarb. Se midió la estabilidad implantaria, coeficiente de estabilidad del implante (ISQ), a través de Ostell® mentor al momento de la instalación (ISQ1) y luego en la conexión (ISQ2) y el Torque de Inserción a través de el motor Osseoset®: 17 implantes Tipo Tornillo de área de contacto promedio aproximada de 237 mm2 (3.75/15 mm; 3.75/13 mm) y 17 implantes Tipo Cónico de área de contacto promedio aproximada de 226 mm2 (4.3/13 mm; 4.3/16 mm). Resultados: Los promedios de ISQ1 e ISQ2 y Torque de Inserción respectivamente para implantes de área contacto aproximada de 237 mm2 fue de 71,3 ISQ1, 66,6 ISQ2 y 44,52 Ncm; para implantes de 226 mm2 fue de 75 ISQ1, 72,5 ISQ2 y 48,82 Ncm. Conclusión: Los implantes de área contacto promedio aproximada de 226 mm2 (Tipo Cónicos) presentan estabilidad primaria y secundaria significativamente mayor a los de área contacto promedio aproximada de 237 mm2 (Tipo Tornillo). (Promedio ISQ1: p = 0.0473; Torque de Inserción: p = 0.0031 y Promedio ISQ2: p = 0.0039)(AU)


Subject(s)
Humans , Dental Implants , Resonance Frequency Analysis , Jaw/injuries , Bone and Bones , Prospective Studies , Longitudinal Studies , Orthodontic Appliance Design/instrumentation , Clinical Study
17.
Artrosc. (B. Aires) ; 30(1): 41-52, 2023.
Article in Spanish | LILACS, BINACIS | ID: biblio-1427241

ABSTRACT

La patología ósea subcondral incluye una amplia gama de patologías, como la artrosis, las fracturas por insuficiencia espontánea, la osteonecrosis y los traumatismos articulares. Todas muestran hallazgos típicos de imágenes de resonancia magnética (RM) denominados lesiones de la médula ósea (LMO). Sin embargo, la etiología y la evolución de las LMO en múltiples afecciones aún no están claras. Además, todavía no existe un protocolo de tratamiento estándar de oro para las LMO, es por esto que se están probando una variedad de modalidades de tratamiento con la esperanza de que puedan reducir el dolor y detener la progresión de la enfermedad. Nuestro propósito es presentar una revisión sobre los conceptos actuales para el diagnóstico y tratamiento de las LMO. Se realizó una revisión de la literatura que incluyó búsquedas en las bases de datos PubMed, Cochrane y Medline utilizando las siguientes palabras clave: lesiones de médula ósea subcondral, hueso subcondral, subcondroplastia, concentrado de médula ósea, plasma rico en plaquetas (PRP) y aumento óseo subcondral. Podemos concluir que el uso de nuevas técnicas biológicas para tratar las LMO, como el PRP y las células de la médula ósea, ha mostrado resultados clínicos prometedores. La investigación futura de las LMO será necesaria para abordar mejor las diferentes patologías y determinar las estrategias terapéuticas adecuadas. Todavía se necesitan estudios randomizados y controlados de alta calidad junto a revisiones sistemáticas para generar guías y recomendaciones para el tratamiento de las LMO.


Subchondral bone pathology includes a wide range of pathologies, such as osteoarthritis, spontaneous insufficiency fractures, osteonecrosis, and trauma. They show typical magnetic resonance imaging (MRI) findings termed bone marrow lesions (BMLs). However, the etiology and evolution of BMLs in multiple conditions remains unclear. There is still no gold standard treatment protocol in treating BML, and a variety of treatment modalities have been tested in the hope that they might reduce pain and stop disease progression.Our purpose was to write a current concepts review about diagnosis and treatment options for BMLs. A literature review was performed that included searches of PubMed, Cochrane, and Medline databases using the following keywords: Bone marrow lesions, subchondral bone, subchondroplasty, bone marrow concentrate, platelet-rich plasma (PRP), subchondral bone augmentation.The use of novel biologic techniques to treat BMLs, such as PRP and Bone Marrow Cells, has yielded promising clinical outcomes. Future research of BMLs will be mandatory to address the different pathologies better and determining appropriate treatment strategies. There is still a need for high-quality RCTs studies and systematic reviews in the future to enhance further treatment strategy in preventing or treating BMLs of the knee.


Subject(s)
Osteochondritis , Bone and Bones , Bone Marrow , Cartilage, Articular , Knee Joint
18.
Chinese Journal of Medical Instrumentation ; (6): 651-658, 2023.
Article in Chinese | WPRIM | ID: wpr-1010256

ABSTRACT

3D printing technology has great advantages in small batch and personalized customization, so it has attracted much attention in the biomedical field. The consumables available for 3D printing include polymer, metal, ceramic and derived materials. Biomedical ceramics, with high melting point and poor toughness, are the most difficult materials to be used in 3D printing. The progress of 3D printing ceramic preparation process using ceramic powder, ceramic slurry, ceramic wire, ceramic film and other different raw materials as consumables are reviewed, and the surface roughness, size, density and other parameters of ceramics prepared by SLS, 3DP, DIW, IJP, SL, DLP, FDM, LOM and other different processes are compared. The study also summarizes the clinical application status of 3D printed bioceramics in the field of hard tissue repair such as bone tissue engineering scaffolds and dental prostheses. The SL ceramic additive manufacturing technology based on the principle of UV polymerization has better manufacturing precision, forming quality and the ability to prepare large-size parts, and can also endow bioceramics with better biological properties, mechanical properties, antibacterial, tumor treatment and other functions by doping trace nutrients and surface functional modification. Compared with the traditional subtractive manufacturing process, the bioceramics prepared by 3D printing not only have good mechanical properties, but also often have better biocompatibility and osteoconductivity.


Subject(s)
Bone and Bones , Ceramics , Printing, Three-Dimensional , Tissue Engineering , Tissue Scaffolds
19.
Chinese Journal of Biotechnology ; (12): 942-960, 2023.
Article in Chinese | WPRIM | ID: wpr-970415

ABSTRACT

Collagen, which widely exists in skin, bone, muscle and other tissues, is a major structural protein in mammalian extracellular matrix. It participates in cell proliferation, differentiation, migration and signal transmission, plays an important role in tissue support and repair and exerts a protective effect. Collagen is widely used in tissue engineering, clinical medicine, food industry, packaging materials, cosmetics and medical beauty due to its good biological characteristics. This paper reviews the biological characteristics of collagen and its application in bioengineering research and development in recent years. Finally, we prospect the future application of collagen as a biomimetic material.


Subject(s)
Animals , Collagen/analysis , Tissue Engineering/methods , Extracellular Matrix/metabolism , Biomimetic Materials/chemistry , Bone and Bones , Tissue Scaffolds , Mammals/metabolism
20.
Chinese Journal of Pathology ; (12): 147-152, 2023.
Article in Chinese | WPRIM | ID: wpr-970149

ABSTRACT

Objective: To investigate the clinicopathological features, diagnosis and differential diagnosis of pediatric myofibroma/myofibromatosis of the soft tissue and bone. Methods: All cases of pediatric myofibroma/myofibromatosis of the soft tissue and bone diagnosed between January 2011 and December 2018 were retrieved from the surgical pathology records in the Department of Pathology, Beijing Jishuitan Hospital, Beijing, China. Clinical and radiological data were collected. H&E and immunohistochemistry were used to examine histological and immunophenotypic features and to make the diagnosis and differential diagnosis. The relevant literature was also reviewed. Results: Twenty-eight cases of pediatric myofibroma/myofibromatosis of the soft tissue and bone were respectively collected. The patients' ages ranged from 2 months to 14 years, with a mean age of 7 years. There were 7 females and 21 males. There were 12 cases located in soft tissue, including the finger (n=9), upper arm (n=1) and foot (n=2). There were 14 cases located in the bone of limb, including the femur (n=8), tibia (n=4), clavicle (n=2), fibula (n=2) and radius (n=1). There were 2 cases of myofibromatosis involving multiple bones. Radiology showed lytic lesions in the bone. The proliferation of spindle-shaped myofibroblasts arranged in fascicles with indistinct eosinophilic cytoplasm and bland nuclei, with no pleomorphism and cytological atypia. The characteristic histologic structure was the biphasic nodular growth pattern with cellular and paucicellular regions. The tumors might arrange in a hemangiopericytoma-like pattern. The stroma varied between dense fibrosis and myxoid changes. The reactive new bone formation and inflammatory cell infiltration also existed. Immunohistochemical study showed that the SMA was positive. The surgical resections were performed. One of the patients had tumor recurrence as a result of 11-month follow-up. Conclusions: The pediatric myofibroma/myofibromatosis of the soft tissue and bone is a very rare benign tumor and has a good prognosis. It has a characteristic morphology and its differential diagnosis from other spindle cell tumors could be made with the immunohistochemical analysis.


Subject(s)
Child , Female , Humans , Infant , Male , Child, Preschool , Adolescent , Bone and Bones/pathology , Diagnosis, Differential , Leiomyoma , Myofibroma/diagnosis , Myofibromatosis/diagnosis
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