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1.
Rev. bras. oftalmol ; 81: e0028, 2022. graf
Article in English | LILACS | ID: biblio-1376785

ABSTRACT

ABSTRACT We report an unusual case of brucellosis presented with headache, diminished vision, papillitis and multiple peripapillary hemorrhages accompanied by subretinal fluid extending up to macula. Diagnosis of brucellosis was made based on positive polymerase chain reaction of cerebrospinal fluid sample for Brucella species DNA, accompanied by a raised titer of anti-brucella antibodies. Patient showed remarkable improvement on triple drug therapy in form of doxycycline, rifampicin and ceftriaxone.


RESUMO Relatamos um caso incomum de brucelose apresentada com cefaleia, visão diminuída, papilite e múltiplas hemorragias peripapilares acompanhadas por fluido sub-retinal, estendendo-se até a mácula. O diagnóstico de brucelose foi feito com base na reação em cadeia da polimerase positiva de amostra de líquido cefalorraquidiano para DNA de espécies de Brucella, acompanhada por um título elevado de anticorpos antibrucela. O paciente apresentou melhora notável com a terapia tripla com drogas na forma de doxiciclina, rifampicina e ceftriaxona.


Subject(s)
Humans , Female , Aged , Brucellosis/diagnosis , Brucellosis/drug therapy , Eye Infections, Bacterial/diagnosis , Eye Infections, Bacterial/drug therapy , Ophthalmoscopy , Rifampin/therapeutic use , Ceftriaxone/therapeutic use , Brucella/isolation & purification , Fluorescein Angiography , Cerebrospinal Fluid/microbiology , Papilledema , Polymerase Chain Reaction , Doxycycline/therapeutic use , Tomography, Optical Coherence
2.
Rev. bras. ciênc. vet ; 28(3): 151-155, jul./set. 2021. il.
Article in English | LILACS, VETINDEX | ID: biblio-1366554

ABSTRACT

Leptospira spp. and Brucella abortus are bacterial pathogens that can infect humans and animals. The present study aimed to detect anti-Leptospira and anti-B. abortus antibodies and verified the presence of factors associated with seropositivity in cats. One hundred and eighty serum samples were collected from domestic cats (Felis silvestris catus) from the urban area of the municipality of Araguaína-Tocantins by phlebocentesis of the cephalic and jugular veins. The samples were subjected to detection of anti-Leptospira and anti-B.abortus antibodies, respectively, by microscopic seroagglutination and buffered acidified antigen testing, followed by confirmation by the 2-mercaptoethanol test and slow seroagglutination in tubes. Data from the epidemiological questionnaire (the age, sex, origin, breed, and presence of clinical signs) were analyzed using Epi Info® software with seropositivity data found to search for associated factors using the chi-square test. In the present study, the prevalence of Leptospira spp. was 5.56% (10/180). However, no sample was reactive to B. abortus. None of the studied variables were associated with seropositivity for the pathogens evaluated. Therefore, there is contact between Leptospira spp. and the feline population of the municipality, indicating the possibility of the circulation of pathogenic serovars and that the presence of anti-Leptospira antibodies does not depend on the variables analyzed.


Leptospira spp. e Brucella abortus são patógenos bacterianos que podem infectar humanos e animais. O presente estudo teve como objetivo detectar anticorpos anti-Leptospira e anti-B.abortus e verificar a presença de fatores associados com a soropositividade em gatos. Foram coletadas 180 amostras de soro de gatos domésticos (Felis silvestris catus) da zona urbana do município de Araguaína-Tocantins por flebocentese das veias cefálica e jugular. As amostras foram submetidas à detecção de anticorpos anti-Leptospira e anti-B. abortus, respectivamente, por soroaglutinação microscópica e teste do antígeno acidificado tamponado, seguido de confirmação pelo teste de 2-mercaptoetanol e soroaglutinação lenta em tubos. Os dados do questionário epidemiológico (idade, sexo, procedência, raça e presença de sinais clínicos) foram analisados no software Epi Info® com os dados de soropositividade encontrados para pesquisa de fatores associados pelo teste do qui-quadrado. No presente estudo, a prevalência de Leptospira spp. foi de 5,56% (10/180). No entanto, nenhuma amostra foi reativa para B. abortus. Nenhuma das variáveis estudadas foi associada com a soropositividade para os patógenos avaliados. Portanto, há contato entre Leptospiraspp. e a população felina do município, indicando a possibilidade de circulação de sorovares patogênicos e que a presença de anticorpos anti-Leptospira independe das variáveis analisadas.


Subject(s)
Animals , Cats , Brucellosis/veterinary , Cats/immunology , Leptospira , Antibodies, Bacterial , Brucella , Seroepidemiologic Studies
3.
Arq. bras. med. vet. zootec. (Online) ; 73(3): 583-588, May-June 2021. ilus
Article in English | LILACS, VETINDEX | ID: biblio-1278349

ABSTRACT

The aim of this study was to investigate the occurrence of diseases in free-ranging wild canids that were roadkill on highways in the State of Espírito Santo, Brazil. PCR tests were performed for the detection of Brucella sp., Babesia sp., Rangelia sp., and Hepatozoon sp. in the spleen. Morphological evaluation and identification of parasites was performed in the liver and lung. Twenty specimens of C. thous were necropsied at the Animal Pathology Sector of the Veterinary Hospital of the Universidade Federal do Espírito Santo. Tissue samples were processed for histopathological examination and polymerase chain reaction (PCR) analysis. There was no PCR amplification of genomic DNA sequences of Brucella sp., Babesia sp., Rangelia sp., and Hepatozoon sp. using DNA extracted from the spleen as template. Histologically, lesions associated with parasitism by Platynosomum sp. and Angiostrongylus sp. were observed in the liver and lung, respectively. This is the first report of Platynosomum sp. and Angiostrongylus sp. parasitism in C. thous in the state of Espírito Santo, Brazil. Therefore, this study demonstrated parasitism of crab-eating foxes by Platynosomum sp. and Angiostrongylus sp. Importantly, no evidence of infection with Brucella sp., Babesia sp., Rangelia sp., and Hepatozoon sp. was obtained by PCR analysis.(AU)


O objetivo deste estudo foi investigar a ocorrência de doenças em canídeos silvestres de vida livre que foram atropelados em rodovias no estado do Espírito Santo, Brasil. Testes de PCR foram realizados para a detecção de Brucella sp., Babesia sp., Rangelia sp. e Hepatozoon sp. no baço. A avaliação morfológica e a identificação de parasitas foram realizadaa no fígado e no pulmão. Vinte espécimes de C. thous foram necropsiados no Setor de Patologia Animal do Hospital Veterinário da Universidade Federal do Espírito Santo. Amostras de tecido foram processadas para exame histopatológico e análise de reação em cadeia da polimerase (PCR). Não houve amplificação por PCR das sequências de DNA genômico de Brucella sp., Babesia sp., Rangelia sp. e Hepatozoon sp. usando-se DNA extraído do baço como modelo. Histologicamente, lesões associadas ao parasitismo por Platynosomum sp. e Angiostrongylus sp. foram observadas no fígado e no pulmão, respectivamente. Este é o primeiro relato de Platynosomum sp. e Angiostrongylus sp. parasitismo em C. thous no estado do Espírito Santo, Brasil. Portanto, este estudo demonstrou parasitismo de cachorro-do-mato por Platynosomum sp. e Angiostrongylus sp. É importante detacar que não há evidências de infecção por Brucella sp., Babesia sp., Rangelia sp. e Hepatozoon sp. por análise de PCR.(AU)


Subject(s)
Animals , Babesia/isolation & purification , Brucella/isolation & purification , Canidae/blood , Angiostrongylus/isolation & purification , Autopsy/veterinary , Spleen/virology , Accidents, Traffic , Polymerase Chain Reaction/veterinary , Liver/parasitology , Lung/parasitology , Animals, Wild/blood
4.
Infectio ; 24(4): 259-261, oct.-dic. 2020.
Article in Spanish | LILACS, COLNAL | ID: biblio-1114879

ABSTRACT

Resumen La brucelosis, principal zoonosis a nivel mundial tiene alta prevalencia en varios países de Latinoamérica. Se asocia con la exposición a ganado infectado por distintas especies del género Brucella. B. melitensis la más virulenta para el humano, causa con frecuencia complicaciones de predominio osteoarticular. En Colombia se cree que la infección por B. melitensis es una entidad ausente, a pesar de su plausibilidad biológica en nuestro contexto; sin embargo, son escasos los estudios sobre su ocurrencia y mínimo el índice de sospecha de la enfermedad, por lo cual creemos está subdiagnosticada. Presentamos el primer caso confirmado de brucelosis por B. melitensis en Colombia en una joven embarazada, con diagnóstico incidental, en quien el análisis retrospectivo de su cuadro clínico alertó sobre puntos clave que pueden impactar en el diagnóstico y tratamiento oportuno de la enfermedad. Se plantean preguntas de prevalencia real de esta entidad en Colombia.


Summary Brucellosis, the principal zoonoses globally is highly prevalent in different countries of Latin America. It is associated with the exposition of livestock infected with different Brucella species, being B. melitensis the most virulent for humans, and frequently causing osteoarticular complications. In Colombia it is believed that B. melitensis infection is an absent entity, despite its biological plausibility in our context; however, there are few studies on its occurrence and a minimum index of suspicion of the disease, which is why we believe it is underdiagnosed. We present the first confirmed case of brucellosis by B. melitensis in Colombia diagnosed in a young pregnant patient, with an incidental diagnosis, in whom a retrospective analysis of her clinical outcome warned of key points that may impact on the diagnosis and timely treatment of the disease. We present several questions surrounding the real prevalence of this entity in Colombia.


Subject(s)
Humans , Female , Pregnancy , Adolescent , Brucellosis , Zoonoses , Brucella melitensis , Brucella , Ethnicity , Colombia , Livestock , Infections
5.
Pesqui. vet. bras ; 40(8): 604-613, Aug. 2020. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1135665

ABSTRACT

Brucellosis is a relevant zoonotic disease for which the most important tool for control is vaccination of susceptible animals. Assessment of vaccine efficacy in natural hosts is based on prevention of abortion and Brucella infection in organs of immunized animals. A meta-analysis of experimental vaccination of Brucella spp. natural hosts was performed, including 45 PubMed and/or Scopus-indexed publications, representing 116 individual experiments. Difference of risk was calculated as an indicator of protection, and a temporal analysis (1980-2016) demonstrated that experimental vaccines tested on natural hosts provided levels of protection that were stable over the past decades. The meta-regression model developed in this study included different vaccine categories (attenuated, inactivated, mutant, subunit, and vectored) considering the difference of risk as the dependent variable. The subcutaneous route of vaccination provided better protection when compared to the intramuscular and oral routes of vaccination. Surprisingly, inactivated vaccines provided better protection than live naturally attenuated vaccine strains (spontaneous mutations) that were considered the reference, whereas subunit vaccines provided lower levels of protection. This is the first meta-analysis of Brucella vaccinology in the natural hosts. These results are useful for the development of new vaccination protocols for controlling animal brucellosis.(AU)


Brucelose é uma doença zoonótica relevante, para a qual a vacinação de animais susceptíveis é a ferramenta mais importante de controle. Avaliação da eficácia vacinal em hospedeiros naturais é baseada na prevenção de aborto e da colonização de órgãos pela Brucella spp. em animais imunizados. Foi realizada meta-análise de estudos de vacinação experimental de Brucella spp. em hospedeiros naturais, incluindo 45 publicações indexadas pela PubMed e/ou Scopus, representando 116 experimentos individuais. Diferença de risco foi calculada como indicador de proteção e uma análise temporal (1980-2016) demonstrou que vacinas experimentais testadas em hospedeiros naturais promoveram níveis de proteção que foram estáveis ao longo das últimas décadas. O modelo de meta-regressão desenvolvido neste estudo incluiu diferentes categorias de vacinas (atenuada, inativada, mutante, subunidade e vetorial) considerando a diferença de risco como variável dependente. A via de vacinação subcutânea promoveu melhor proteção quando comparada às vias intramuscular e oral. Surpreendentemente, vacinas inativadas promoveram melhor proteção que vacinas vivas atenuadas (com mutações espontâneas) que foram consideradas como referência, enquanto vacinas de subunidades promoveram menor proteção. Este é o primeiro estudo de meta-análise da vacinologia de Brucella em hospedeiros naturais. Estes resultados são úteis para o desenvolvimento de novos protocolos vacinais para controle de brucelose animal.(AU)


Subject(s)
Animals , Cattle , Brucellosis/prevention & control , Immunogenicity, Vaccine , Brucella , Vaccinology , Immunity
6.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 1039-1046, May-June, 2020. ilus, tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1129747

ABSTRACT

O objetivo deste comunicado é desenvolver um método quantitativo PCR em tempo real, baseado em guia molecular (MB) (MB-qPCR) para detecção de infecção por espécies de Brucella, e avaliar seu potencial de utilização clínica. Os primers e as sondas MB foram desenhados para amplificação específica e determinação de sequência conservada do código do gene para os primeiros 58-aa da proteína de membrana externa OMP-2a, que é compartilhada em cinco espécies de Brucella epidêmicas. A avaliação metodológica foi realizada por análise de sensibilidade, especificidade, coeficiente de variação intra e inter, e a linearidade do qPCR. O potencial diagnóstico foi avaliado comparando-se o método qPCR desenvolvido com ensaios de exames bacteriológicos convencionais, incluindo os testes de soroaglutinação convencionais (SATs) e os testes do Rosa Bengala (RBPTs). O método exibiu alta sensibilidade (tão baixo quanto 50 cópias) e grande faixa de linearidade (102-108 cópias). Nenhuma reação cruzada foi encontrada com bactéria clínica comum. A sensibilidade diagnóstica foi superior ao exame bacteriológico, e a especificidade diagnóstica foi superior ao SAT ou ao RBPT. Um método MB-qPCR altamente sensível e específico para DNA de Brucella foi estabelecido com sucesso, provando ser uma ferramenta útil no diagnóstico molecular de brucelose.(AU)


Subject(s)
Brucella/isolation & purification , Genome, Bacterial , Real-Time Polymerase Chain Reaction/methods
7.
Rev. cuba. invest. bioméd ; 39(1): e336, ene.-mar. 2020. graf
Article in Spanish | LILACS, CUMED | ID: biblio-1126572

ABSTRACT

Introducción: El género Brucella está incluido en la familia Brucellaceae que pertenece al orden Rhizobiales y es reconocido por su alto grado de patogenicidad. Las bacterias de este género son responsables de la brucelosis, enfermedad que ha sido reportada como una de las zoonosis más importantes a nivel mundial por su incidencia en el ganado y el hombre. Los estudios previos para la clasificación taxonómica del género, se han basado fundamentalmente en el análisis del gen 16S ARNr. Sin embargo, pocas investigaciones se han dirigido a la identificación de marcadores moleculares que distingan a sus miembros de otros grupos de bacterias. Objetivo: Identificar inserciones en secuencias de proteínas conservadas, que pudieran ser utilizados como marcadores moleculares para la taxonomía y diagnóstico de especies del género Brucella. Métodos: Las secuencias homólogas de las proteínas analizadas fueron obtenidas de bases de datos internacionales y, posteriormente, alineadas con el programa ClustalX2, para ello fueron considerados los parámetros sugeridos en la literatura. Resultados: Se identificaron inserciones en las proteínas oxoglutarato deshidrogenasa (componente E1) y ADN ligasa A específicas del género Brucella. Conclusiones: Las inserciones halladas pueden ser empleadas como complemento a los métodos tradicionales de clasificación taxonómica y para el diagnóstico molecular de bacterias incluidas en el género Brucella(AU)


Introduction: Brucella is a genus from the Brucellaceae family, Rhizobiales order. This genus is recognized for its high pathogenicity. Brucella bacteria cause brucellosis, a disease reported as one of the most important zoonoses worldwide due to its incidence in cattle and people. Previous studies on taxonomic classification of the genus have been mainly based on the analysis of gene 16S rDNA. However, few studies have been aimed at identification of molecular markers distinguishing its members from other groups of bacteria. Objective: Identify insertions in preserved protein sequences which could be used as molecular markers for the taxonomy and diagnosis of species from the Brucella genus. Methods: The homologous sequences for the proteins analyzed were obtained from international databases and aligned with the software ClustalX2, considering the parameters suggested in the literature. Results: Insertions were identified in the proteins oxoglutarate dehydrogenase (component E1) and DNA ligase A, specific of the genus Brucella. Conclusions: The insertions found may be used as complements to the traditional methods for taxonomic classification and for the molecular diagnosis of bacteria from the genus Brucella(AU)


Subject(s)
Humans , Sequence Homology , Ketoglutarate Dehydrogenase Complex , Brucella/pathogenicity , Genetic Markers/genetics
8.
Article in Chinese | WPRIM | ID: wpr-826387

ABSTRACT

To compare the differences in apparent diffusion coefficient (ADC) and fractional anisotropy (FA) between brucella spondylitis (BS) groups at different stages before treatment and the normal control group and to evaluate the change trend of ADC value and FA value at different time points before and after treatment. Totally 53 patients suspected of BS by conventional magnetic resonance imaging (MRI) and later confirmed as BS patients by serological tests were enrolled in this study. These patients underwent conventional MRI and diffusion tensor imaging scans,and the ADC value and FA value were measured. Independent sample test was used to compare the ADC value and FA value between the BS group and the control group,the ADC value and FA value between the BS group at each stage. Repeated measurement ANOV was used to compare the ADC values and FA values at different time points before and after treatment. FA imaging showed that the color code of BS was different from that of the normal control group,and the color code of FA imaging showed increased singal. The ADC values of BS in the acute,subacute,and chronic stages [(1.45±0.02)×10 mm /s,(1.35±0.03)×10 mm /s,(1.26±0.05)×10 mm /s,respectively] were significantly higher than those in the control group [(1.06±0.09) ×10 mm /s](=2.538,=0.009;=1.998,=0.032;=1.575,=0.004),and the FA value (0.55±0.02,0.65±0.03,0.71±0.04,respectively) were significantly lower than those of the control group (0.78±0.02) (=2.440,=0.012; =1.847,=0.041;=2.102,=0.003). Repeated measurement analysis showed that there were statistically significant differences in ADC values and FA values at different time points before and after treatment in the acute,subacute,and chronic stages (ADC:=12.100,<0.001;=8.439,=0.005;=9.704,=0.004,respectively;FA:=7.080,=0.002;=6.607;=0.003;=8.868,=0.001,respectively). The ADC values at different time points after treatment were significantly lower than those before treatment or at a previous time point after treatment (=332.14,<0.001),and the FA values were significantly higher than those before treatment or at a previous time point after treatment (=134.26,<0.001). FA color code can intuitively display differences in BS and normal vertebral bodies and show change of color code before and after treatment. Also,the ADC values and FA values can quantitatively reveal differences between BS and normal vertebral body in different time points and quantify BS vertebral lesion changes before and after treatment. In particular,in BS patients who are recovering from treatment,it can quantify microscopic edema. Therefore,diffusion tensor imaging may be useful objective indicator in evaluating the effectiveness of a specific treatment for BS.


Subject(s)
Anisotropy , Brucella , Brucellosis , Diagnostic Imaging , Diffusion Magnetic Resonance Imaging , Diffusion Tensor Imaging , Humans , Spondylitis , Diagnostic Imaging , Microbiology
9.
Arq. Inst. Biol ; 86: e0582018, 2019.
Article in English | LILACS, SES-SP, VETINDEX, SESSP-IPPROD, SES-SP | ID: biblio-1009484

ABSTRACT

Among the diseases that affect equines, bacterial diseases play an important role from a health and economic point of view, especially leptospirosis and brucellosis. The study aimed to provide information on the occurrence of anti-Leptospira spp. and anti-Brucella abortus antibodies in donkeys of São Paulo state. We found a frequency of 62.4% (53/85) antibodies against Leptospira spp. The donkeys were not seropositive for Brucella spp.(AU)


Entre as doenças que acometem os equídeos, as enfermidades bacterianas assumem um papel importante do ponto de vista sanitário e econômico, destacando-se a leptospirose e a brucelose. O estudo teve como objetivo fornecer informações sobre a ocorrência de anticorpos anti-Leptospira spp. e anti-Brucella abortus em jumentos no estado de São Paulo. Estimou-se que 62,4% (53/85) dos animais apresentavam anticorpos anti-Leptospira spp. Os jumentos estudados não foram sororreagentes contra a Brucella spp.(AU)


Subject(s)
Animals , Bacterial Infections , Brucellosis , Equidae , Leptospirosis , Brucella , Serologic Tests/methods , Leptospira/pathogenicity
10.
Laboratory Medicine Online ; : 177-180, 2019.
Article in Korean | WPRIM | ID: wpr-760497

ABSTRACT

Catabacter hongkongensis is an anaerobic gram-positive coccobacillus that was first isolated in Hong Kong. It is infectious and causes high mortality in patients with rare but underlying diseases. Alistipes indistinctus is an anaerobic gram-negative coccobacillus. This bacterium is a common member of the human intestinal microbiota. We report a case of C. hongkongensis and A. indistinctus isolated from blood cultures of a patient with acute appendicitis. A 35-year-old female patient with no specific medical history was admitted to the hospital due to abdominal pain, vomiting, nausea, and diarrhea experienced on the day before admission. On admission, laboratory tests revealed leukocytosis, neutropenia, and elevated C–reactive protein and procalcitonin levels. Following an abdominal computed tomography showing acute appendicitis with suspected perforation, emergency surgery was performed. Growth was observed in two anaerobic blood culture bottles after four days. After further culturing of the bacteria on Brucella Blood Agar, two types of bacteria were obtained. The two bacterial isolates, one gram-positive and one gram-negative, were unable to be identified using matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Thus, 16S rRNA gene sequence analysis was performed, resulting in identification of the bacteria as C. hongkongensis and A. indistinctus. The patient was administered antibiotics and discharged two days after surgery. Although MALDI-TOF MS enables fast and accurate identification of bacteria, C. hongkongensis and A. indistinctus were not listed in the spectral library, and 16S rRNA gene sequence analysis was useful for identifying the two bacteria.


Subject(s)
Abdominal Pain , Adult , Agar , Anti-Bacterial Agents , Appendicitis , Bacteria , Brucella , Diarrhea , Emergencies , Female , Gastrointestinal Microbiome , Genes, rRNA , Hong Kong , Humans , Leukocytosis , Mass Spectrometry , Mortality , Nausea , Neutropenia , Sequence Analysis , Vomiting
11.
Article in English | WPRIM | ID: wpr-758937

ABSTRACT

Brucella is an intracellular pathogen that invades a host and settles in its immune cells; however, the mechanism of its intracellular survival is unclear. Modification of small ubiquitin-related modifier (SUMO) occurs in many cellular activities. E2 conjugating enzyme 9 (Ubc9) is the only reported ubiquitin-conjugating enzyme that links the SUMO molecule with a target protein. Brucella's intracellular survival mechanism has not been studied with respect to SUMO-related proteins and Ubc9. Therefore, to investigate the relationship between Brucella melitensis 16M and SUMO, we constructed plasmids and cells lines suitable for overexpression and knockdown of SUMO1 and Ubc9 genes. Brucella 16M activated SUMO1/Ubc9 expression in a time-dependent manner, and Brucella 16M intracellular survival was inhibited by SUMO1/Ubc9 overexpression and promoted by SUMO1/Ubc9 depletion. In macrophages, Brucella 16M-dependent apoptosis and immune factors were induced by SUMO1/Ubc9 overexpression and restricted by SUMO1/Ubc9 depletion. We noted no effect on the expressions of SUMO1 and Ubc9 in B. melitensis 16M lipopolysaccharide-prestimulated mouse RAW264.7 macrophages. Additionally, intracellular survival of the 16M△VirB2 mutant was lower than that of Brucella 16M (p < 0.05). VirB2 can affect expression levels of Ubc9, thereby increasing intracellular survival of Brucella in macrophages at the late stage of infection. Collectively, our results demonstrate that B. melitensis 16M may use the VirB IV secretion system of Brucella to interact with SUMO-related proteins during infection of host cells, which interferes with SUMO function and promotes pathogen survival in host cells.


Subject(s)
Animals , Apoptosis , Brucella melitensis , Brucella , Immunologic Factors , Macrophages , Mice , Plasmids
12.
Arq. Ciênc. Vet. Zool. UNIPAR (Online) ; 21(4): 149-150, out-dez. 2018.
Article in Portuguese | LILACS, VETINDEX | ID: biblio-986999

ABSTRACT

Dentre as zoonoses responsáveis pelas maiores perdas socioeconômicas na bovinocultura brasileira, a brucelose possui significativo destaque, sendo considerada uma antropozoonose causada pelas bactérias do gênero Brucella spp. Essas bactérias possuem potencial para infectar diversos mamíferos, inclusive, existem registros de brucelose em cetáceos (baleias e golfinhos). Nos bovinos, as principais maneiras de transmissão se dão pelas descargas bacterianas presentes nos restos de materiais provenientes de abortos de vacas positivas para brucelose, mas também pode ser transmitida pelo leite, cópula, fômites, solo e até a água. As vacas continuam liberando as bactérias por cerca de 30 dias após o parto ou aborto. Para diagnóstico são utilizados testes diretos ou indiretos preconizados pelo Programa Nacional de Controle e Erradicação da Brucelose e da Tuberculose Animal (PNCEBT). O objetivo deste trabalho foi avaliar o perfil epidemiológico de Brucelose bovina na região noroeste do estado do Paraná de 2014 a 2016. Entre os anos de 2014 a 2016, foram utilizados dados de exames realizados por Médico Veterinário devidamente registrado e habilitado para a realização do diagnóstico. Entre bovinos machos e fêmeas foram testados 12.640 animais, na região noroeste do estado Paraná, sendo o teste utilizado o do antígeno acidificado tamponado (AAT). 26 animais foram positivos para brucelose, perfazendo um percentual de 0,2 % do rebanho analisado. Apesar dos baixos índices de animais infectados pela Brucella spp. apresentados nessa pesquisa o controle e uma possível erradicação da brucelose se tornam indispensáveis devido à fácil transmissão e às perdas econômicas provocadas, principalmente pelos abortos ocorridos nos últimos trimestres das gestações.(AU)


Brucellosis features among the zoonoses responsible for greatest socioeconomic losses in the Brazilian cattle farming. Considered an anthropozoonosis, its etiological agents are bacteria of the Brucella spp genus. Such bacteria have the potential to infect several mammals, with records of brucellosis even in cetaceans (whales and dolphins). In cattle, it is mainly transmitted through bacterial discharges present in the remains of materials coming from abortions tested positive for brucellosis. Nevertheless, it can also be transmitted by milk, copulation, blood transfusions, fomites, soil and even water. Cows continue releasing the bacteria for about 30 days after calving or abortion. Direct or indirect tests as recommended by the National Program for the Control and Eradication of Brucellosis and Animal Tuberculosis (PNCEBT) can be used for diagnosis. The objective of this study was to assess the epidemiological profile of bovine brucellosis in the northwestern region of the State of Paraná from 2014 to 2016. Between 2014 and 2016, data from examinations performed by a veterinarian duly registered and qualified to perform the diagnosis were used. A total of 12,640 animals in the northwest region of the state of Paraná were tested, both male and female, using the acidified buffered antigen (ABA). A total of 26 animals tested positive for brucellosis, which corresponds to 0.2% of the studied herd. Despite the low rates of animals infected with Brucella spp. presented in this study, the control and possible eradication of brucellosis is essential due to its easy transmission and extensive economic losses mainly caused by abortions occurring during the last quarters of pregnancies.(AU)


Entre las zoonosis responsables por las mayores pérdidas socioeconómicas en la bovinocultura brasileña, la brucelosis posee significativo destaque, es considerada una antropozoonosis causada por bacterias del género Brucella spp. Esas bacterias poseen potencial para infectar diversos mamíferos, incluso, existen registros de brucelosis en cetáceos (ballenas y delfines). En los bovinos, las principales formas de transmisión se dan por las descargas bacterianas presentes en los restos de materiales provenientes de abortos de vacas positivas para brucelosis, también puede ser transmitida por la leche, cópula, fómites, suelo e incluso el agua. Las vacas continúan liberando las bacterias durante unos 30 días después del parto o aborto. Para el diagnóstico se utilizan pruebas directas o indirectas preconizadas por el Programa Nacional de Control y Erradicación de Brucelosis y de la Tuberculosis Animal (PNCEBT). El objetivo de esta investigación ha sido evaluar el perfil epidemiológico de Brucelosis bovina en la región noroeste del estado de Paraná de 2014 a 2016. Entre los años de 2014 a 2016 se utilizaron datos de exámenes realizados por Médico Veterinario debidamente registrado y habilitado para la realización del diagnóstico. Entre bovinos machos y hembras se probaron 12.640 animales, en la región noroeste del estado de Paraná, la prueba utilizada fue antígeno acidificado tamponado (AAT). 26 animales atestaron positivos para brucelosis, lo que significó el 0,2% del rebaño analizado. A pesar de los bajos índices de animales infectados por Brucella spp. presentados en esa investigación, el control y una posible erradicación de la brucelosis se vuelven indispensables debido a su fácil transmisión y las pérdidas económicas provocadas, principalmente por los abortos ocurridos en los últimos trimestres de las gestaciones.(AU)


Subject(s)
Animals , Male , Female , Cattle , Brucellosis, Bovine/classification , Brucellosis, Bovine/epidemiology , Zoonoses , Brucella
13.
Biomédica (Bogotá) ; 38(supl.2): 30-36, ago. 2018. tab
Article in Spanish | LILACS | ID: biblio-974004

ABSTRACT

Introducción. Cada año mueren, aproximadamente, tres millones de personas como consecuencia de las enfermedades transmitidas por alimentos. El queso artesanal fresco que se produce y distribuye en la región Caribe colombiana es un producto autóctono de los departamentos de Córdoba, Sucre, Bolívar, Atlántico, Magdalena, Cesar y La Guajira. Su consumo masivo aumenta el riesgo de infección con salmonelosis, listeriosis y brucelosis debido a que es elaborado con una tecnología muy rústica, con leche de vaca no pasteurizada, sin procedimientos estandarizados e higiénicos, y a que su almacenamiento no es adecuado. Objetivo. Detectar la presencia de Salmonella spp., Listeria spp. y Brucella spp. en muestras de queso artesanal costeño fresco procedente de los departamentos de la región Caribe colombiana. Materiales y métodos. Mediante reacción en cadena de la polimerasa en tiempo real (qPCR), se analizaron 27 muestras de queso proveniente de cinco departamentos de la región Caribe: Atlántico (n=6), Bolívar (n=2), Córdoba (n=1), Magdalena (n=16) y Sucre (n=2). Del total de las muestras, 17 eran de queso blando, cinco de queso semiduro y cinco de queso duro. Resultados. En el 62,9 % de las muestras se detectó Salmonella spp. (17/27), en el 70,4 %, Listeria spp. (19/27), y en el 22,2 %, Brucella spp. (6/27). Las muestras provenían principalmente del departamento del Magdalena y, en 62,5 % (10/16) de ellas, se encontró Salmonella spp. y Listeria spp., en tanto que en el 50 % (3/6) de las muestras del departamento del Atlántico se detectó Brucella spp. Conclusión. Los resultados evidenciaron la presencia de estos microorganismos en todas las muestras de queso costeño blando.


Introduction: Each year approximately 3 million people die as the result of foodborne diseases. The fresh artisan (handmade) cheese produced and distributed in the Colombian Caribbean region is a native product from the departments of Córdoba, Sucre, Bolívar, Atlántico, Magdalena, Cesar, and La Guajira. Its mass consumption increases the risk of infection with Salmonella spp., Listeria spp., and Brucella spp., as it is made with a very rustic technology, with unpasteurized cow milk, without standardized and hygienic procedures and its storage is inadequate. Objective: To detect the presence of Salmonella spp., Listeria spp., and Brucella spp. in samples of fresh artisan cheese from the Colombian Caribbean region. Materials and methods: Twenty-seven samples of cheese from five departments of the Caribbean Region (Atlántico (n=6), Bolívar (n=2), Córdoba (n=1), Magdalena (n=16), and Sucre (n=2)) were analyzed by real-time polymerase chain reaction (qPCR). Seventeen of the samples corresponded to soft cheese, five to semi-hard cheese and five to hard cheese. Results: In 62.9% (17/27) of the samples we detected Salmonella spp., in 70.4% (19/27), Listeria spp., and in 22.2% (6/27), Brucella spp., mainly from the department of Magdalena. In 62.5% (10/16) of the samples we detected Salmonella spp. and Listeria spp. while in the department of Atlántico, 50% (3/6) of the samples corresponded to Brucella spp. Conclusion: The results confirmed the presence of these microorganisms in all the samples of soft cheese from the Colombian Caribbean region.


Subject(s)
Foodborne Diseases/epidemiology , Salmonella , Brucella , Cheese , Polymerase Chain Reaction , Listeria
14.
Acta bioquím. clín. latinoam ; 52(1): 61-64, mar. 2018. tab
Article in Spanish | LILACS | ID: biblio-886161

ABSTRACT

La brucelosis es una enfermedad muy extendida por todo el mundo. El diagnóstico se realiza mediante el uso de diferentes técnicas, entre ellas el aislamiento del agente etiológico o por serología detectando anticuerpos específicos. Todos estos métodos están estandarizados y validados en general para las distintas especies animales de interés zootécnico. En animales silvestres pueden utilizarse los mismos procedimientos serológicos, pero cada uno debe ser validado para la especie animal estudiada. El objetivo de este trabajo fue comprobar si la técnica de Polarización Fluorescente (FPA) utilizada para la determinación de anticuerpos contra Brucella en bovinos puede ser usada para el diagnóstico de Brucella en el armadillo (Chaetophractus villosus). Para ello se tomaron 150 muestras de sangre de Chaetophractus villosus. Veinticuatro muestras fueron positivas por medio de las técnicas de antígeno bufferado en placa, seroaglutinación lenta en tubo, 2-mercaptoetanol y fijación del complemento. Las mismas muestras resultaron positivas a la técnica de Polarización Fluorescente, estableciéndose un límite de corte de 82 mP. El índice Kappa registrado fue de 1 para todos los tests de diagnóstico comparados con FPA (IC: 0,84-1).


Brucellosis is a disease widespread throughout the world. The diagnosis is made through the use of different techniques including the isolation of the etiological agent or by serology detecting specific antibodies. All these methods are standardized and validated in general for animal species of zoo-technical interest. In wild animals, serological procedures may be used, but each should be validated for the particular animal species studied. The objective of this work was to verify if the technique of Fluorescent Polarization used for the determination of antibodies against Brucella in ruminants can be used for the diagnosis of Brucella in Chaetophractus villosus. To this aim, s 150 blood samples of Chaetophractus villosus were taken. Twenty-four samples were positive by the techniques of Antigen Buffered in Plate, serum agglutination test (SAT), 2-mercaptoethanol (2-ME) agglutination test and complement fixation test (CFT). The same samples were also positive by Fluorescent Polarization, and a cut-off limit of 82 mP was established. The KAPPA index was 1 for all diagnostic tests compared to FPA (CI: 0.84-1).


A brucelose é uma doença muito espalhada pelo mundo. O diagnóstico é feito por meio de diferentes técnicas, incluindo o isolamento do agente etiológico ou por sorologia detectando anticorpos específicos. Todos esses métodos são padronizados e validados em geral para diferentes espécies animais de interesse zootécnico. Em animais silvestres podem ser utilizados os mesmos métodos serológicos, mas cada um tem de ser validado para a espécie de animal estudada. O objectivo deste estudo foi testar se a técnica de Polarização Fluorescente utilizada para a determinação de anticorpos contra Brucella em bovinos pode ser utilizada para o diagnóstico de Brucella em tatu (Chaetophractus villosus). Para isso, 150 amostras de sangue de Chaetophractus villosus foram coletadas. Vinte e quatro amostras foram positivas por técnicas de antígeno bufferado em placa, soroaglutinação lenta em tubo, 2-mercaptoetanol e fixação do complemento. As mesmas amostras foram positivas para a técnica de Polarização Fluorescente, estabelecendo um limite de corte de 82 mP. O índice Kappa registrado foi de 1 para todos os testes de diagnóstico em comparação com FPA (CI: 0,84-1).


Subject(s)
Brucella , Diagnosis , Fluorescence Polarization/methods , Serology , Serology/instrumentation , Gram-Negative Bacteria
15.
LMJ-Lebanese Medical Journal. 2018; 66 (1): 46-48
in English | IMEMR | ID: emr-170974

ABSTRACT

A 63-year-old female patient presented for infected total knee prosthesis by brucella spp. several years after undergoing knee replacement arthroplasty. She underwent a two-stage revision surgery and was treated wih gentamicin, rifampicin and doxycycline for a total duration of four months


Subject(s)
Humans , Female , Middle Aged , Brucellosis/diagnosis , Brucella/isolation & purification , Brucellosis/drug therapy , Infections
16.
Article in English | WPRIM | ID: wpr-741500

ABSTRACT

Brucellosis is one of the common zoonoses caused by Brucella abortus (B. abortus). However, little has been reported on factors affecting invasion of B. abortus into host cells. To investigate cell-type dependent invasion of B. abortus, phagocytic RAW 264.7 and THP-1 cells and non-phagocytic HeLa cells were infected with wild-type and mutant B. abortus, and their invasion efficiencies were compared. The invasion efficiencies of the strains were cell-type dependent. Wild-type B. abortus invasion efficiency was greater in phagocytic cells than in epithelial cells. The results also indicated that there are different factors involved in the invasion of B. abortus into phagocytic cells.


Subject(s)
Brucella abortus , Brucella , Brucellosis , Epithelial Cells , HeLa Cells , Humans , Phagocytes , Zoonoses
17.
Infection and Chemotherapy ; : 149-152, 2018.
Article in English | WPRIM | ID: wpr-721489

ABSTRACT

Brucellosis is a zoonotic infection that is usually transmitted from cattle to humans through ingestion of animal milk, direct contact with animal parts, or inhalation of aerosolized particles. In Korea, brucellosis seem to be transmitted through close contact with blood, fetus, urine, and placenta of domestic cow that has been infected by Brucella abortus, or inhalation of B. arbortus while examining or slaughtering cow. Brucella melitensis infection is rare in Korea and there have been no reported cases of B. melitensis originating from other countries until now. This report details a case of complicated brucellosis with infective spondylitis in a 48-year-old male construction worker recently returned from Iraq. Infection with B. melitensis was confirmed using 16s rRNA sequencing and omp31 gene analysis. The patient was successfully treated using a combination of rifampin, doxycycline, and streptomycin, in accordance with WHO guidelines. This is the first reported case of complicated brucellosis with infective spondylitis in Korea caused by B. melitensis originating from Iraq.


Subject(s)
Animals , Brucella abortus , Brucella melitensis , Brucella , Brucellosis , Cattle , Doxycycline , Eating , Fetus , Humans , Inhalation , Iraq , Korea , Male , Middle Aged , Middle East , Milk , Placenta , Rifampin , Spondylitis , Streptomycin , Zoonoses
18.
Infection and Chemotherapy ; : 149-152, 2018.
Article in English | WPRIM | ID: wpr-721994

ABSTRACT

Brucellosis is a zoonotic infection that is usually transmitted from cattle to humans through ingestion of animal milk, direct contact with animal parts, or inhalation of aerosolized particles. In Korea, brucellosis seem to be transmitted through close contact with blood, fetus, urine, and placenta of domestic cow that has been infected by Brucella abortus, or inhalation of B. arbortus while examining or slaughtering cow. Brucella melitensis infection is rare in Korea and there have been no reported cases of B. melitensis originating from other countries until now. This report details a case of complicated brucellosis with infective spondylitis in a 48-year-old male construction worker recently returned from Iraq. Infection with B. melitensis was confirmed using 16s rRNA sequencing and omp31 gene analysis. The patient was successfully treated using a combination of rifampin, doxycycline, and streptomycin, in accordance with WHO guidelines. This is the first reported case of complicated brucellosis with infective spondylitis in Korea caused by B. melitensis originating from Iraq.


Subject(s)
Animals , Brucella abortus , Brucella melitensis , Brucella , Brucellosis , Cattle , Doxycycline , Eating , Fetus , Humans , Inhalation , Iraq , Korea , Male , Middle Aged , Middle East , Milk , Placenta , Rifampin , Spondylitis , Streptomycin , Zoonoses
19.
Article in English | WPRIM | ID: wpr-758849

ABSTRACT

Salmonella is an intracellular pathogen with a cellular infection mechanism similar to that of Brucella, making it a suitable choice for use in an anti-Brucella immune boost system. This study explores the efficacy of a Salmonella Typhimurium delivery-based combination vaccine for four heterologous Brucella antigens (Brucella lumazine synthase, proline racemase subunit A, outer-membrane protein 19, and Cu/Zn superoxide dismutase) targeting brucellosis in goats. We inoculated the attenuated Salmonella delivery-based vaccine combination subcutaneously at two different inoculation levels; 5 × 10⁹ colony-forming unit (CFU)/mL (Group B) and 5 × 10¹⁰ CFU/mL (Group C) and challenged the inoculations with virulent Brucella abortus at 6 weeks post-immunization. Serum immunoglobulin G titers against individual antigens in Salmonella immunized goats (Group C) were significantly higher than those of the non-immunized goats (Group A) at 3 and 6 weeks after vaccination. Upon antigenic stimulation, interferon-γ from peripheral blood mononuclear cells was significantly elevated in Groups B and C compared to that in Group A. The immunized goats had a significantly higher level of protection as demonstrated by the low bacterial loads in most tissues from the goats challenged with B. abortus. Relative real-time polymerase chain reaction results revealed that the expression of Brucella antigens was lower in spleen, kidney, and lung of immunized goats than of non-immunized animals. Also, treatment with our combination vaccine ameliorated histopathological lesions induced by the Brucella infection. Overall, the Salmonella Typhimurium delivery-based combination vaccine was effective in delivering immunogenic Brucella proteins, making it potentially useful in protecting livestock from brucellosis.


Subject(s)
Animals , Bacterial Load , Brucella abortus , Brucella Vaccine , Brucella , Brucellosis , Goats , Immunoglobulin G , Kidney , Livestock , Lung , Proline , Real-Time Polymerase Chain Reaction , Salmonella typhimurium , Salmonella , Spleen , Stem Cells , Superoxides , Vaccination
20.
Article in English | WPRIM | ID: wpr-758812

ABSTRACT

An anti-Brucella vaccine candidate comprised of purified Brucella lipopolysaccharide (LPS) and a cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported previously. Each vector constitutively expressed highly conserved Brucella antigens (rB), viz., lumazine synthase (BLS), proline racemase subunit A, outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined a relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice, with the ST-Omp19-, ST-BLS-, LPS-, and ST-SOD-immunized mice showing much less burden. Indirect enzyme-linked immunosorbent assay-based assay also confirmed the induction of antigen-specific immunoglobulin G for each antigen delivered. In a route-wise comparison of the combined vaccine candidate, intraperitoneal (IP), intramuscular (IM), and subcutaneous immunizations revealed an indication of highly efficient routes of protection. Splenocytes of mice immunized via IM and IP routes showed significant relative expression of IL-17 upon antigenic pulsing. Taken together, each of the Brucella antigens delivered by ST successfully induced an antigen-specific immune response, and it was also evident that an individual antigen strain can confer a considerable degree of protection. More effective protection was observed when the candidate was inoculated via IP and IM routes.


Subject(s)
Animals , Brucella , Brucellosis , Immunization , Immunoglobulin G , Interleukin-17 , Membranes , Mice , Proline , Salmonella typhimurium , Salmonella , Superoxide Dismutase , Vaccination
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