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1.
Odontol. vital ; (31): 59-66, jul.-dic. 2019. graf
Article in Spanish | LILACS, BBO | ID: biblio-1091429

ABSTRACT

Resumen En la actualidad tanto el consumo de jugos, como de bebidas gaseosas, es muy común en la dieta diaria de la población infantil, estas ofrecen diferentes sabores, además de un alto contenido de azúcar. En este estudio se hizo un análisis de la capacidad buffer de la saliva ante la ingesta de diferentes tipos de bebidas consideradas saludables y no saludables. Se utilizaron dos tipos de instrumentos, un examen clínico aplicado a 62 estudiantes para determinar la prevalencia de caries según ICDAS y un segundo instrumento que consta de una tabla aplicada a los mismos 62 estudiantes con la finalidad de analizar la capacidad buffer de la saliva mediante la toma del pH a intervalos de 15, 35 y 45 minutos después de ingerir las bebidas. Como conclusión se obtuvo que para las bebidas consideradas no saludables el pH tardó más en neutralizarse, mientras que la bebida saludable logró su neutralización más rápido, con una baja prevalencia de caries.


Abstract Nowadays the consumption of juices, and soft drinks, is very common in the children's diet, they offer different flavours and a high content of sugar. In this study, an analysis of the buffer capacity of the saliva was made in view of the intake of different types of beverages considered healthy and unhealthy. Two types of instruments were used, a clinical test applied to 62 students to determine the prevalence of caries according to ICDAS and a second instrument that consists of a chart applied to the same 62 students with the purpose of analyzing the buffer capacity by taking the pH on intervals of 15, 35 and 45 minutes after drinking the beverages. As a conclusion, it was obtained that for the drinks considered unhealthy, the pH took longer to neutralize, while the healthy drink achieved its neutralization faster, with a low prevalence of caries.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Saliva/chemistry , Buffers , Food and Beverages/analysis , Dental Caries/chemically induced , Sugar-Sweetened Beverages/adverse effects
2.
Pesqui. bras. odontopediatria clín. integr ; 19(1): 4107, 01 Fevereiro 2019. tab
Article in English | LILACS, BBO | ID: biblio-997911

ABSTRACT

Objective: To evaluate the influence of gustatory stimuli on the buffering capacity of saliva. Material and Methods: The buccal ph of 18 male volunteers aged 18-35 years was measured after a mouthwash with 20 ml of water as a control, and in individual disposable cups they collected the saliva for two minutes. Then, each of chewed bubble gum with sugar for two minutes, discarding the gum and made new collection of saliva, for two minutes in other disposable cups individualized. After collection, each volunteer was again subject to regular brushing with toothpaste and waited another ten minutes. The same procedure was repeated with all other substances. Salivary buffer capacity was determined by Ericsson technique. Data were submitted to analysis of variance and the means were compared by the Scott-Knott grouping test and Mann-Whitney test at 5% probability. Estimates of Pearson correlations were calculated in order to determine possible associations between the variables. Results: It was not found statistically significant differences between the initial pH variation and after eating food (p>0.05), or between gustatory stimulation and variation of salivary buffer capacity (p>0.05). Conclusion: There is no influence of gustatory stimulus aroma and flavor on the variation of salivary buffer capacity.


Subject(s)
Humans , Male , Adolescent , Adult , Saliva/metabolism , Taste Buds , Diet , Taste Perception , Hydrogen-Ion Concentration , Salivation , Brazil , Buffers , Analysis of Variance , Statistics, Nonparametric
3.
Article in Korean | WPRIM | ID: wpr-760017

ABSTRACT

PURPOSE: The purpose was to investigate the literature on depression among South Korean nurses in order to identify overall trends regarding depression. METHODS: Forty-five papers published between 1998 and 2017 in three Korean databases-RISS4U, DBPIA, and KISS-were analyzed. RESULTS: Depression showed significant positive associations with job stress, burnout, intent to leave, and somatic symptoms such as fatigue and trouble sleeping. Poor work environments such as staff shortage, work overload, and shift work were also positively associated with depression. Higher job satisfaction, resilience, self-efficacy, self-esteem, and social support were negatively correlated with and acted as significant buffers against depression. CONCLUSION: These results indicate that healthcare policymakers must pay more attention to the management and prevention of nurses' depression. In order to effectively do so, poor work conditions, which involve long working hours, high patient-to-nurse ratios, and extreme workloads, should be improved. Simultaneously, nursing managers should implement initiatives such as counseling and competency improvement programs, and stress management to reduce and prevent depression among nurses and strengthen their positive competency. Also, further research is required to clarify the prevalence of depression in South Korean nurses, with more meta-analyses also required to identify variations in depression rates owing to various factors.


Subject(s)
Buffers , Counseling , Delivery of Health Care , Depression , Fatigue , Job Satisfaction , Nursing , Prevalence
4.
Article in English | WPRIM | ID: wpr-740004

ABSTRACT

BACKGROUND: The purpose of this study was to compare the pain perception and anesthetic efficacy of 2% lignocaine with 1:200,000 epinephrine, buffered lignocaine, and 4% articaine with 1:200,000 epinephrine for the inferior alveolar nerve block. METHODS: This was a double-blind crossover study involving 48 children aged 5–10 years, who received three inferior alveolar nerve block injections in three appointments scheduled one week apart from the next. Pain on injection was assessed using the Wong-Baker Faces pain scale and the sound eye motor scale (SEM). Efficacy of anesthesia was assessed by subjective (tingling or numbness of the lip, tongue, and corner of mouth) and objective signs (pain on probing). RESULTS: Pain perception on injection assessed with Wong-Baker scale was significantly different between buffered lignocaine and lignocaine (P < 0.001) and between buffered lignocaine and articaine (P = 0.041). The onset of anesthesia was lowest for buffered lignocaine, with a statistically significant difference between buffered lignocaine and lignocaine (P < 0.001). Moreover, the efficacy of local analgesia assessed using objective signs was significantly different between buffered lignocaine and lignocaine (P < 0.001) and between lignocaine and articaine. CONCLUSION: Buffered lignocaine was the least painful and the most efficacious anesthetic agent during the inferior alveolar nerve block injection in 5–10-year-old patients.


Subject(s)
Analgesia , Anesthesia , Appointments and Schedules , Buffers , Carticaine , Child , Cross-Over Studies , Epinephrine , Humans , Hypesthesia , Lidocaine , Lip , Mandibular Nerve , Pain Perception , Tongue
5.
Article in English | WPRIM | ID: wpr-763574

ABSTRACT

OBJECTIVE: Loneliness is a specific risk factor for depressive symptoms and suicidal behavior. The present study examined whether the serum oxytocin level would interact with social support and buffers loneliness and hypothalamic-pituitary-adrenal (HPA)-axis activity in drug-naïve patients with major depressive disorder (MDD). METHODS: Twenty-six patients with MDD (male:female = 3:23; mean age, 45.54 ± 12.97 years) were recruited. The 17-item Hamilton Depression Rating Scale, UCLA Loneliness Scale and self-reported Measurement of Support Function Questionnaire were administered. Serum oxytocin and cortisol levels were assessed using a commercial immunoassay kits. RESULTS: In MDD patients, a negative association was found between degrees of social support and loneliness (β = −0.39, p = 0.04). The interaction between social support and serum oxytocin level was negatively associated with loneliness (β = −0.50, p = 0.017) and serum cortisol level (β = −0.55, p = 0.020) after adjusting for age. Follow-up analyses showed that the association between higher social support and lower loneliness was observed only in the higher-oxytocin group (r = −0.75, p = 0.003) but not in the lower group (r = −0.19, p = 0.53). The significance remained after further adjusting for sex and depression severity. CONCLUSION: Low oxytocin level is a vulnerability factor for the buffering effect of social support for loneliness and aberrant HPA-axis activity in MDD patients.


Subject(s)
Buffers , Depression , Depressive Disorder, Major , Follow-Up Studies , Humans , Hydrocortisone , Immunoassay , Loneliness , Oxytocin , Risk Factors
6.
Electron. j. biotechnol ; 19(5): 2-11, Sept. 2016. ilus
Article in English | LILACS | ID: lil-797331

ABSTRACT

The buffering effect of acetate on hydrogen production during glucose fermentation by Ethanoligenens harbinense B49 was investigated compared to phosphate, a widely used fermentative hydrogen production buffer. Specific concentrations of sodium acetate or phosphate were added to batch cultures, and the effects on hydrogen production were comparatively analyzed using a modified Gompertz model. Adding 50 mM acetate or phosphate suppressed the hydrogen production peak and slightly extended the lag phase. However, the overall hydrogen yields were 113.5 and 108.5 mmol/L, respectively, and the final pH was effectively controlled. Acetate buffered against hydrogen production more effectively than did phosphate, promoting cell growth and preventing decreased pH. At buffer concentrations 100-250 mM, the maximum hydrogen production was barely suppressed, and the lag phase extended past 7 h. Therefore, although acetate inhibits hydrogen production, using acetate as a buffer (like phosphate) effectively prevented pH drops and increased substrate consumption, enhancing hydrogen production.


Subject(s)
Gram-Positive Bacteria/metabolism , Hydrogen/metabolism , Acetates/analysis , Phosphates/analysis , Buffers , Biofuels , Fermentation , Batch Cell Culture Techniques , Glucose , Hydrogen-Ion Concentration
7.
Article in English | WPRIM | ID: wpr-146114

ABSTRACT

Colloidal particle size is an important characteristic that allows mapping sentinel nodes in lymphoscintigraphy. This investigation aimed to introduce different ways of making a 99mTc-tin colloid with a size of tens of nanometers. All agents, tin fluoride, sodium fluoride, poloxamer-188, and polyvinylpyrrolidone (PVP), were mixed and labeled with 99mTc. Either phosphate or sodium bicarbonate buffers were used to adjust the pH levels. When the buffers were added, the size of the colloids increased. However, as the PVP continued to increase, the size of the colloids was controlled to within tens of nanometers. In all samples, phosphate buffer added PVP (30 mg) stabilized tin colloid (99mTc-PPTC-30) and sodium bicarbonate solution added PVP (50 mg) stabilized tin colloid (99mTc-BPTC-50) were chosen for in vitro and in vivo studies. 99mTc-BPTC-50 (100 nm) mainly accumulated in the liver. When a rabbit was given a toe injection, the node uptake of 99mTc-PPTC-30 decreased over time, while 99mTc-BPTC-50 increased. Therefore, 99mTc-BPTC-50 could be a good candidate radiopharmaceutical for sentinel node detection. The significance of this study is that nano-sized tin colloid can be made very easily and quickly by PVP.


Subject(s)
Animals , Buffers , Cell Line, Tumor , Humans , Lymph Nodes/diagnostic imaging , Lymphatic Metastasis , Metal Nanoparticles/chemistry , Mice , Neoplasms, Experimental/diagnostic imaging , Particle Size , Povidone/chemistry , Rabbits , Radiopharmaceuticals/chemical synthesis , Reproducibility of Results , Sensitivity and Specificity , Technetium Compounds/chemistry , Tin/chemistry , Tin Compounds/chemistry
8.
Article in English | WPRIM | ID: wpr-137593

ABSTRACT

Recent cohort studies have relied on exposure prediction models to estimate individual-level air pollution concentrations because individual air pollution measurements are not available for cohort locations. For such prediction models, geographic variables related to pollution sources are important inputs. We demonstrated the computation process of geographic variables mostly recorded in 2010 at regulatory air pollution monitoring sites in South Korea. On the basis of previous studies, we finalized a list of 313 geographic variables related to air pollution sources in eight categories including traffic, demographic characteristics, land use, transportation facilities, physical geography, emissions, vegetation, and altitude. We then obtained data from different sources such as the Statistics Geographic Information Service and Korean Transport Database. After integrating all available data to a single database by matching coordinate systems and converting non-spatial data to spatial data, we computed geographic variables at 294 regulatory monitoring sites in South Korea. The data integration and variable computation were performed by using ArcGIS version 10.2 (ESRI Inc., Redlands, CA, USA). For traffic, we computed the distances to the nearest roads and the sums of road lengths within different sizes of circular buffers. In addition, we calculated the numbers of residents, households, housing buildings, companies, and employees within the buffers. The percentages of areas for different types of land use compared to total areas were calculated within the buffers. For transportation facilities and physical geography, we computed the distances to the closest public transportation depots and the boundary lines. The vegetation index and altitude were estimated at a given location by using satellite data. The summary statistics of geographic variables in Seoul across monitoring sites showed different patterns between urban background and urban roadside sites. This study provided practical knowledge on the computation process of geographic variables in South Korea, which will improve air pollution prediction models and contribute to subsequent health analyses.


Subject(s)
Air Pollution , Altitude , Buffers , Cohort Studies , Family Characteristics , Geographic Information Systems , Geography , Housing , Information Services , Korea , Seoul , Transportation
9.
Article in English | WPRIM | ID: wpr-137592

ABSTRACT

Recent cohort studies have relied on exposure prediction models to estimate individual-level air pollution concentrations because individual air pollution measurements are not available for cohort locations. For such prediction models, geographic variables related to pollution sources are important inputs. We demonstrated the computation process of geographic variables mostly recorded in 2010 at regulatory air pollution monitoring sites in South Korea. On the basis of previous studies, we finalized a list of 313 geographic variables related to air pollution sources in eight categories including traffic, demographic characteristics, land use, transportation facilities, physical geography, emissions, vegetation, and altitude. We then obtained data from different sources such as the Statistics Geographic Information Service and Korean Transport Database. After integrating all available data to a single database by matching coordinate systems and converting non-spatial data to spatial data, we computed geographic variables at 294 regulatory monitoring sites in South Korea. The data integration and variable computation were performed by using ArcGIS version 10.2 (ESRI Inc., Redlands, CA, USA). For traffic, we computed the distances to the nearest roads and the sums of road lengths within different sizes of circular buffers. In addition, we calculated the numbers of residents, households, housing buildings, companies, and employees within the buffers. The percentages of areas for different types of land use compared to total areas were calculated within the buffers. For transportation facilities and physical geography, we computed the distances to the closest public transportation depots and the boundary lines. The vegetation index and altitude were estimated at a given location by using satellite data. The summary statistics of geographic variables in Seoul across monitoring sites showed different patterns between urban background and urban roadside sites. This study provided practical knowledge on the computation process of geographic variables in South Korea, which will improve air pollution prediction models and contribute to subsequent health analyses.


Subject(s)
Air Pollution , Altitude , Buffers , Cohort Studies , Family Characteristics , Geographic Information Systems , Geography , Housing , Information Services , Korea , Seoul , Transportation
10.
Braz. oral res. (Online) ; 29(1): 1-6, 2015. tab, ilus
Article in English | LILACS | ID: lil-777225

ABSTRACT

A low pH and a high titratable acidity of juices and cola-based beverages are relevant factors that contribute to dental erosion, but the relative importance of these properties to maintain salivary pH at demineralizing levels for long periods of time after drinking is unknown. In this crossover study conductedin vivo, orange juice, a cola-based soft drink, and a 10% sucrose solution (negative control) were tested. These drinks differ in terms of their pH (3.5 ± 0.04, 2.5 ± 0.05, and 5.9 ± 0.1, respectively) and titratable acidity (3.17 ± 0.06, 0.57 ± 0.04 and < 0.005 mmols OH- to reach pH 5.5, respectively). Eight volunteers with a normal salivary flow rate and buffering capacity kept 15 mL of each beverage in their mouth for 10 s, expectorated it, and their saliva was collected after 15, 30, 45, 60, 90, and 120 s. The salivary pH, determined using a mini pH electrode, returned to the baseline value at 30 s after expectoration of the cola-based soft drink, but only at 90 s after expectoration of the orange juice. The salivary pH increased to greater than 5.5 at 15 s after expectoration of the cola drink and at 30 s after expectoration of the orange juice. These findings suggest that the titratable acidity of a beverage influences salivary pH values after drinking acidic beverages more than the beverage pH.


Subject(s)
Humans , Beverages , Citrus sinensis/chemistry , Saliva/chemistry , Buffers , Carbonated Beverages , Cross-Over Studies , Citric Acid/chemistry , Cola/chemistry , Hydrogen-Ion Concentration , Reference Values , Time Factors , Titrimetry , Tooth Erosion/chemically induced
11.
Article in Spanish | LILACS | ID: lil-746938

ABSTRACT

OBJETIVO Determinar la capacidad buffer de la saliva al ser añadida a distintas bebidas energéticas comercializadas en Chile, mediante mediciones de pH in vitro. MÉTODOS Fue requerida la participación de 3 pacientes jóvenes sistémicamente sanos, sin enfermedad de las glándulas salivales. Las muestras de saliva estimulada fueron obtenidas de cada paciente, las cuales fueron mezcladas y almacenadas en una sola muestra. Se seleccionaron 13 bebidas energéticas comercializadas a nivel nacional. Un total de 5 mL de cada bebida energética se distribuyó en 4 tubos Falcon. Se midió el pH de cada una de las bebidas energéticas, de la saliva y del agua potable. Se añadió 1 mL de agua potable al tubo Falcon número 1 y 1 mL de saliva a los 3 tubos restantes, cada 3 min hasta completar 13 mL de solución en cada uno (38% vol./vol.). Las mediciones de pH fueron realizadas en cada 1 mL añadido (saliva/agua), para permitir al ph-metro registrar de manera correcta. RESULTADOS Los rangos de pH para las bebidas energéticas van desde pH 2,42 ± 0,008 (Battery Gingered®), hasta pH 3,44 ± 0,005 (Battery Sugar Free®). La saliva en promedio tuvo un valor de pH 7,99 y el agua potable de 7,05. La bebida que más logró aumentar el pH, luego de agregar la saliva, fue la bebida Speed® que llegó a un valor de pH 4,38, mientras la que logró menos fue la bebida Quick Energy®, con un valor de pH 3,37. CONCLUSIÓN La capacidad buffer de la saliva logró aumentar entre 17 y 54% el pH de las bebidas energéticas analizadas en este estudio. Sin embargo, no pudo neutralizar los bajos niveles de pH de estas bebidas más allá de un pH final de 4,38, que es crítico para la estructura dentaria.


OBJECTIVE To determine, using in vitro pH measurements, the buffering capacity of saliva when added to different energy drinks sold in Chile. METHOD The participation of 3 young and systemically healthy patients, with no diseases of the saliva glands, was obtained. Samples of stimulated saliva where obtained from each patient and then mixed and stored as one sample. The study used 13 energy drinks sold nationwide, with 5 mL of each one being distributed into 4 Falcon tubes. The pH of each of the energy drinks, the saliva sample, and drinking water was measured. 1 mL of drinking water was added into Falcon tube number 1, and 1 mL of saliva into the 3 remaining every 3 min until completing 13 mL of solution in each one (38% vol./vol.). The pH measurements where performed upon adding each 1 mL (saliva/water) to allow the pH meter to correctly register the data. RESULTS The pH levels for energy drinks range between pH 2.42 ± 0.008 (Battery Gingered®), to pH 3.44 ± 0.005 (Battery Sugar Free®). The saliva had a mean pH value of 7.99, and 7.05 for drinkable water. The energy drink that achieved a higher increase in pH level was Speed®, reaching a value of pH 4.38, while the energy drink that increased the pH level the least was Quick Energy®, only reaching a pH of 3.37. CONCLUSION The buffering capacity of saliva managed to increase the pH level of energy drinks analyzed in this study between 17 and 54%. However, saliva was unable to neutralize further than 4.38 the low levels of pH in these drinks, thus being critical to dental structure.


Subject(s)
Humans , Male , Adolescent , Adult , Saliva , Energy Drinks/analysis , Energy Drinks/adverse effects , Hydrogen-Ion Concentration , In Vitro Techniques , Buffers
12.
Braz. j. phys. ther. (Impr.) ; 18(6): 513-520, 09/01/2015. graf
Article in English | LILACS | ID: lil-732354

ABSTRACT

BACKGROUND: Knowing the potential for and limitations of information generated using different evaluation instruments favors the development of more accurate functional diagnoses and therapeutic decision-making. OBJECTIVE: To investigate the relationship between the number of compensatory movements when climbing up and going down stairs, age, functional classification and time taken to perform a tested activity (TA) of going up and down stairs in boys with Duchenne muscular dystrophy (DMD). METHOD: A bank of movies featuring 30 boys with DMD performing functional activities was evaluated. Compensatory movements were assessed using the climbing up and going down stairs domain of the Functional Evaluation Scale for Duchenne Muscular Dystrophy (FES-DMD); age in years; functional classification using the Vignos Scale (VS), and TA using a timer. Statistical analyses were performed using the Spearman correlation test. RESULTS: There is a moderate relationship between the climbing up stairs domain of the FES-DMD and age (r=0.53, p=0.004) and strong relationships with VS (r=0.72, p=0.001) and TA for this task (r=0.83, p<0.001). There were weak relationships between the going down stairs domain of the FES-DMD-going down stairs with age (r=0.40, p=0.032), VS (r=0.65, p=0.002) and TA for this task (r=0.40, p=0.034). CONCLUSION: These findings indicate that the evaluation of compensatory movements used when climbing up stairs can provide more relevant information about the evolution of the disease, although the activity of going down stairs should be investigated, with the aim of enriching guidance and strengthening accident prevention. Data from the FES-DMD, age, VS and TA can be used in a complementary way to formulate functional diagnoses. Longitudinal studies and with broader age groups may supplement this information. .


CONTEXTUALIZAÇÃO: Conhecer as potencialidades e limitações das informações geradas por diferentes instrumentos de avaliação favorece o desenvolvimento mais preciso do diagnóstico funcional e da tomada de decisão terapêutica. OBJETIVO : Investigar a relação entre o número de movimentos compensatórios ao subir e descer escadas, idade, classificação funcional e tempo de realização de atividade (TA) em meninos com Distrofia Muscular de Duchenne (DMD). MÉTODO : Foi utilizado banco de filmes de 30 meninos com DMD realizando atividades funcionais. Os movimentos compensatórios foram avaliados pela Escala de Avaliação Funcional para Distrofia Muscular de Duchenne (FES-DMD), domínio subir e descer escada; a idade, mensurada em anos; a classificação funcional foi pesquisada pela Escala de Vignos (EV), e o TA foi cronometrado. Foi utilizado o teste de correlação de Spearman. RESULTADOS : Existe moderada relação entre a FES-DMD-subir escada e a idade (r=0,53, p=0,004) e forte relação com a EV (r=0,72, p=0,001) e TA dessa tarefa (r=0,83, p<0,001). Houve fraca relação entre a FES-DMD-descer escada e a idade (r=0,40, p=0,032), EV (r=0,65, p=0,002) e o TA dessa tarefa (r=0,40, p=0,034). CONCLUSÃO : Esses achados indicam que a avaliação da tarefa de subir escada pode trazer informações mais relevantes sobre a evolução da doença, embora a atividade de descer escada deva ser pesquisada visando à orientação e prevenção de acidentes. A utilização conjunta de dados provenientes da FES-DMD, da idade e do TA pode se complementar para formulação do diagnóstico funcional. Estudos longitudinais e com outras faixas etárias mais amplas podem complementar tal informação. .


Subject(s)
Humans , Male , Prostatic Hyperplasia/metabolism , Receptors, Androgen/metabolism , Binding, Competitive , Buffers , Charcoal , Cytosol/metabolism , Dextrans , Dihydrotestosterone/metabolism , Electrophoresis, Agar Gel , Enzyme Activation/drug effects , Estrenes/metabolism , Metribolone , Molybdenum/pharmacology , Progesterone/metabolism , Protease Inhibitors/pharmacology , Temperature , Tartrates/pharmacology , Testosterone Congeners/metabolism
13.
Perionews ; 8(1): 20-25, jan.-fev. 2014. ilus
Article in Portuguese | LILACS, BBO | ID: lil-708033

ABSTRACT

Objetivos: relatar um caso clínico e analisar a relação do fluxo salivar e da capacidade tampão da saliva na formação do cálculo supragengival em uma paciente com alto grau de formação, através da realização de testes salivares para quantificar essas variáveis. Métodos: foram realizadas três coletas salivares com intervalos de tempo mínimo de um mês entre elas. Para a mensuração do fluxo salivar, a salivação foi estimulada através da utilização de uma lâmina de parafina durante cinco minutos, e o volume obtido foi dividido por cinco, obtendo-se dessa forma a relação mL/min. Para a análise da capacidade tampão da saliva, foi realizada a leitura de pH após a adição de 1 ml da saliva coletada com 3 mL de ácido clorídrico a 0,005 N, seguido pela homogeneização e repouso da mistura por dez minutos. A leitura do valor do pH salivar final foi registrada através de um eletrodo de pH acoplado a um potenciômetro. Resultados: como médias dos valores do fluxo salivar e da capacidade tampão, obteve-se respectivamente 2,13 m L/min e pH 5,26. Conclusão: as variáveis "fluxo salivar e capacidade tampão" mostraram-se sem uma relação direta com a tendência de formação de cálculo dental da paciente. Como fator determinante para o quadro clinico apresentado, pôde-se constatar o baixo grau de instrução sobre higiene bucal, influenciado pelo fator socioeconômico.


Subject(s)
Humans , Female , Adult , Buffers , Dental Calculus , Oral Health , Saliva , Salivation
14.
EMHJ-Eastern Mediterranean Health Journal. 2014; 20 (2): 139-145
in English | IMEMR | ID: emr-159149

ABSTRACT

This study was evaluated the relationship between blood glucose levels and salivary pH and buffering capacity in type II diabetic patients. The sample comprised 210 participants [age ranged 40-60 years]. Based on fasting blood glucose levels the participants were divided into 3 groups: controls with normal blood glucose levels; diabetic patients with levels > 200 mg/dL; and diabetic patients with levels >/= 200 mg/dL. Salivary pH and buffering capacity were determined in a sample of resting [non-stimulated] saliva taken from each participant. Salivary pH levels in diabetic patients with blood glucose levels >/= 200 mg/dL were lower than in the controls and diabetic patients with levels

Subject(s)
Humans , Female , Male , Blood Glucose , Hydrogen-Ion Concentration , Saliva , Buffers
15.
Article in Chinese | WPRIM | ID: wpr-747696

ABSTRACT

OBJECTIVE@#To explore different conditions and buffers of antigen retrieval which affect the CK of SNIP on immunohistochemical staining results.@*METHOD@#Dividing paraffin tissue sections of 11 patients into four groups. Using the Image-Pro Plus Image analyzer and taking five horizons for each section to calculate an average of 200 areas, measured standard optical density of the positive reaction areas.@*RESULT@#It is divided into four groups: high temperature and high pressure citrate buffer retrieval, microwave EDTA buffer retrieval, microwave citrate retrieval, high temperature and high pressure EDTA buffer retrieval. The standard optical density of positive reaction areas respectively express: 0.324 ± 0.051, 0.325 ± 0.056, 0.303 ± 0.061, 0.365 ± 0.023. The rates of CK positive expression with high temperature and high pressure EDTA buffer retrieval is batter than other repairing groups in the same paraffin tissue sections (P < 0.05).@*CONCLUSION@#For the Pan of Sinonasal inverted papilloma, the method of high temperature and high pressure EDTA buffer antigen retrieval can achieve the ideal staining results? which is worth while to promote and maybe as a bet? ter guide of clinic work.


Subject(s)
Antigens , Buffers , Humans , Immunohistochemistry , Methods , Microwaves , Nose Neoplasms , Allergy and Immunology , Papilloma, Inverted , Allergy and Immunology , Paranasal Sinus Neoplasms , Allergy and Immunology , Staining and Labeling , Methods
16.
Indian J Biochem Biophys ; 2013 Dec; 50(6): 570-756
Article in English | IMSEAR | ID: sea-154217

ABSTRACT

Esterification of lauric acid with n-butanol, catalyzed by immobilized Candida antarctica lipase (CAL) in aqueous-organic biphasic solvent system was studied. Effects of various reaction parameters on esterification were investigated, such as type and amount of solvent, amount of buffer, pH, temperature, speed of agitation, amount of enzyme, butanol and lauric acid. The most suitable reaction conditions for esterification were observed at 50ºC and pH 7.0 using 5000 μmoles of lauric acid, 7000 μmoles of butanol, 0.25 ml phosphate buffer, 1 ml of isooctane as the solvent and 50 mg of immobilized enzyme in the reaction medium at agitation speed of 150 rpm. Maximum esterification of 96.36% was acheived in 600 min of reaction time at n-butanol to lauric acid molar ratio of 1: 0.7. Kinetic study for the esterification of lauric acid with n-butanol using immobilized CAL was carried out and the kinetic constants were estimated by using non-linear regression method. The estimated value of Michaelis kinetic constants for butanol (KmBt) and acid (KmAc) were 451.56 (M) and 4.7 × 10-7(M), respectively and the value of dissociation constant (KBt) of the butanol-lipase complex was 9.41 × 107(M). The estimated constants agreed fairly well with literature data.


Subject(s)
Buffers , Butanols/chemistry , Enzymes, Immobilized/metabolism , Esterification , Fungal Proteins/metabolism , Hydrogen-Ion Concentration , Kinetics , Lauric Acids/chemistry , Lipase/metabolism , Solvents/chemistry , Temperature , Water/chemistry
17.
Int. j. odontostomatol. (Print) ; 7(3): 343-349, Dec. 2013. ilus
Article in Spanish | LILACS | ID: lil-696561

ABSTRACT

Gran cantidad de población consume cotidianamente infusiones, como el Té, Manzanilla y Yerba Mate. Diferentes estudios han determinado sus efectos benéficos en los seres humanos, razón por la cual, para este estudio se han seleccionado aquellas infusiones de uso habitual con el fin de caracterizar sus efectos inmediatos sobre las propiedades de la saliva. Con grupos de 37 sujetos sanos, entre 18-23 años, de bajo riesgo cariogénico se obtuvieron 3 muestras de saliva no estimulada: Basal; Post-ingesta de Agua Destilada (Placebo) y Post-ingesta Infusión (Té Negro, Té Verde, Mate, Manzanilla y Manzanilla con Endulzante), respectivamente. Todas las pruebas fueron realizadas bajo condiciones estándar. Se determinó el flujo salival (ml/min), pH mediante pH-metro (PL-600, GOnDO Electronics Co, TW) y capacidad buffer mediante método de Ericsson. Todos los datos se procesaron mediante la prueba ANOVA con el programa Origin 6.0. El promedio de Flujo Salival Basal (0,51 ml/min) tiende a aumentar destacando el efecto de la Manzanilla con Endulzante (0,63 ml/min); el pH basal (7,25) se mantuvo relativamente constante, y la Capacidad Buffer (4,38) también tiende a aumentar destacando la Manzanilla (5,01). El efecto de algunas infusiones es positivo sobre las propiedades salivales, destacando la Infusión de Manzanilla, Manzanilla con Endulzante y Yerba Mate las cuales aumentan significativamente el flujo y la capacidad buffer salival, lo cual sugiere un efecto benéfico en la prevención de caries.


A great number of the population consumes daily a variety of infusions such as Tea, Chamomile and Mate Herb. Different studies have determined their favorable effects in human beings, for this reason those infusions habitually used have been selected for this study, in order to characterize their immediate effects on the saliva properties. We studied groups of 37 healthy subjects, between 18-23 years of age, with low caries risk, and obtained 3 samples of non-stimulated saliva: Basal; Post-ingestion of Distilled Water (Placebo); Post-ingestion of Infusion (Black Tea, Green Tea, Mate Herb, Chamomile and Chamomile with Sucralose). All the tests were realized under standard conditions. We measured, salivary flow (ml/min); pH with pH-meter (PL-600, GOnDO Electronics Co, TW) and buffer capacity with Ericsson's method. All the information was processed with Anova Test in Origin 6.0. Our results showed the average of Salivary Basal Flow (0.51 ml/min) tends to increase standing out the effect of Chamomile with Sucralose (0.63 ml/min), the basal pH (7.25) was maintained relatively constant, and finally the Buffer Capacity (4.38) also tends to increase, emphasizing Chamomile (5.01). The effect of some infusions is positive on the salivary properties, emphasizing the Infusion of Chamomile, Chamomile with Sucralose and Mate Herb, which increase significantly the flow and the salivary buffer capacity. This suggests a favorable effect in the prevention of caries.


Subject(s)
Humans , Male , Adolescent , Adult , Female , Young Adult , Beverages , Salivation , Analysis of Variance , Buffers , Chamomile , Dental Caries/prevention & control , Hydrogen-Ion Concentration , Ilex paraguariensis , Secretory Rate , Tea
18.
Acta Physiologica Sinica ; (6): 83-88, 2013.
Article in Chinese | WPRIM | ID: wpr-333131

ABSTRACT

The variability of peak current of L-type calcium channel (I(Ca,L)) shows an increase in cardiomyocytes after 6 h of preservation when the acutely isolated cardiomyocytes are preserved in a small volume buffer solution. The mechanism of the increased variability of I(Ca,L) is not clear. In order to obtain more accurately and stably experimental data of I(Ca,L), the aim of this study was to observe the pH changes of preservation buffer solution with acutely isolated rat cardiomyocytes, and the effects of pH changes on the shape of cardiomyocytes, the function of mitochondria and the gating property of L-type calcium channel. The results indicated that the pH was kept stable in 100 mL buffer solution, but was decreased from 7.20 to 6.95 in 20 mL buffer solution during 10 h of cardiomyocyte preservation. Therefore, 100 mL or 20 mL preservation solution was used as a normal control or acidotic group, respectively. The ratio of abnormal to normal rod-shaped cardiomyocytes increased in the acidotic group after 6 h of preservation. The acidosis induced a reduction in mitochondrial membrane potential indicated by JC-1 fluorescent probe after 8 h of cardiomyocyte preservation. The acidosis also shifted the autofluorescence of NADPH from blue to green after 8 h of cardiomyocyte preservation. The above changes in mitochondrial function induced a significant decrease in the peak I(Ca,L) and a shift in the clamped voltage at peak I(Ca,L) from +10 mV to 0 mV, after 10 h of cardiomyocyte preservation. These results suggest that the best way to preserve acutely isolated cardiomyocytes is to use a larger volume buffer system. In order to get stable peak I(Ca,L), we need to not only select a normal shape of cardiomyocyte at a bright field but also a blue fluorescent myocyte at an ultraviolet excitation.


Subject(s)
Animals , Buffers , Calcium Channels, L-Type , Physiology , Cells, Cultured , Membrane Potential, Mitochondrial , Myocytes, Cardiac , Physiology , Preservation, Biological , Rats
19.
Pakistan Journal of Pharmaceutical Sciences. 2013; 26 (5): 865-871
in English | IMEMR | ID: emr-138403

ABSTRACT

Two chromatographic methods were developed for analysis ofdiiodohydroxyquinoline [DIHQ] and metronidazole [MTN]. In the first method, diiodohydroxyquinoline and metronidazole were separated on TLC silica gel 60F254 plate using chloroform: acetone: glacial acetic acid [7.5: 2.5: 0.1, by volume] as mobile phase. The obtained bands were then scanned at 254 nm. The second method is a RP-HPLC method in which diiodohydroxyquinoline and metronidazole were separated on a reversed-phase C18 column using water: methanol [60 :40, V/V, PH=3.6] as mobile phase at a flow rate of 0.7 mL.min[-1] and UV detection at 220 nm. The mentioned methods were successfully used for determination of diiodohydroxyquinoline and metronidazole in pure form and in their pharmaceutical formulation


Subject(s)
Iodoquinol/chemistry , Metronidazole/chemistry , Chromatography, High Pressure Liquid , Chromatography, Reverse-Phase , Chromatography, Thin Layer , Hydrogen-Ion Concentration , Technology, Pharmaceutical/methods , Reference Standards , Reproducibility of Results , Solvents/chemistry , Spectrophotometry, Ultraviolet , Buffers , Calibration
20.
Pakistan Journal of Pharmaceutical Sciences. 2013; 26 (5): 961-966
in English | IMEMR | ID: emr-138416

ABSTRACT

An easy, fast and validated RV-HPLC method was invented to quantify donepezil hydrochloride in drug solution and orally disintegrating tablet. The separation was carried out using reversed phase C-18 column [Agilent Eclipse Plus C-18] with UV detection at 268 nm. Method optimization was tested using various composition of organic solvent. The mobile phase comprised of phosphate buffer [0.01M], methanol and acetonitrile [50:30:20, v/v] adjusted to pH 2.7 with phosphoric acid [80%] was found as the optimum mobile phase. The method showed intraday precision and accuracy in the range of 0.24% to -1.83% and -1.83% to 1.99% respectively, while interday precision and accuracy ranged between 1.41% to 1.81% and 0.11% to 1.90% respectively. The standard calibration curve was linear from 0.125 micro g/mL to 16 micro g/mL, with correlation coefficient of 0.9997 +/- 0.00016. The drug solution was stable under room temperature at least for 6 hours. System suitability studies were done. The average plate count was > 2000, tailing factor <1, and capacity factor of 3.30. The retention time was 5.6 min. The HPLC method was used to assay donepezil hydrochloride in tablet and dissolution study of in-house manufactured donepezil orally disintegrating tablet and original Aricept


Subject(s)
Indans/analysis , Chromatography, High Pressure Liquid/standards , Chromatography, Reverse-Phase/standards , Drug Stability , Technology, Pharmaceutical/methods , Acetonitriles/chemistry , Administration, Oral , Buffers , Calibration , Limit of Detection , Methanol/chemistry , Phosphoric Acids/chemistry
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