Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 34
Filter
1.
Braz. arch. biol. technol ; 63(spe): e20190493, 2020. tab, graf
Article in English | LILACS | ID: biblio-1142505

ABSTRACT

Abstract We evaluated the effect of combined Rhizobium tropici, Trichoderma asperellum and plant growth-promoting rhizobacteria (PGPR) in beans crop. The hypothesis that strains of T. asperullum, R. tropici and PGPR combined could improve growth, biomass accumulation and beans yield was tested under greenhouse and field conditions. The treatments consisted of control, mineral nitrogen application and inoculation, isolated and associated with the following microorganisms: Rhizobium tropici, Bacillus subtilis, Trichoderma asperellum and Burkholderia sp. 10N6. Results were evaluated by shoot dry weight (SDW) and root dry weight (RDW), number of nodules and yield components. In greenhouse environment all the microorganisms behaved similarly, and the treatments inoculated with Burkholderia sp. 10N6 (IBu) and R. tropici (IR) stood out regarding the production components. In field conditions the treatments IR and IRTBa presented the highest values of SDW and RDW. Our results suggest that inoculation with R. tropici, T. asperellum and PGPR may promote beans growth and bring benefits to shoot and root accumulation, increase the number of nodules as well as improve yield components, contributing to a sustainable agriculture.


Subject(s)
Phaseolus/growth & development , Phaseolus/microbiology , Bacillus subtilis , Trichoderma , Burkholderia , Rhizobium tropici , Plant Development
2.
Rev. argent. microbiol ; 51(3): 255-258, set. 2019. ilus, tab
Article in Spanish | LILACS | ID: biblio-1041834

ABSTRACT

La espectrometría de masas (EM) (matrix assisted laser desorption ionization-time of flight) MALDI-TOF demostró ser una herramienta robusta para la identificación de numerosos grupos taxonómicos. No obstante, presenta limitaciones. Una ventaja clave de la técnica es la flexibilidad para la incorporación de espectros proteicos de microorganismos ausentes en la base de datos comercial. Dada la prevalencia de Burkholderia contaminans en los pacientes fibroquísticos en Argentina, y a que en ellos es crucial el diagnóstico microbiológico rápido y confiable, la EM MALDI-TOF surge como una herramienta estratégica. El objetivo del trabajo fue desarrollar una base de datos adicional con espectros peptídicos de aislamientos de referencia de B. contaminans. La misma demostró ser exitosa para la identificación del 97% de los aislamientos analizados. Por lo cual la EM MALDI-TOF con la base de datos extendida resultó ser una herramienta útil para la identificación y diferenciación de otras especies relacionadas a B. contaminans.


MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry (MS) proved to be a robust tool for the identification of numerous taxonomic groups. However, it has limitations. A key advantage of this technique is the flexibility for the incorporation of protein profiles of microorganisms not included in the commercial database. Due to the prevalence of Burkholderia contaminans in fibrocystic patients in Argentina and the fact that rapid and reliable microbiological diagnosis is crucial in them, MALDI-TOF MS emerges as a strategic tool. The aim of this work was to develop an additional database with peptide spectra of reference isolates of B. contaminans. This database demonstrated to be successful for the identification of 97% of the isolates analyzed. Therefore, MALDI-TOF MS with the extended database was a useful tool for the identification and differentiation of other related species to B. contaminans.


Subject(s)
Humans , Databases, Factual , Bacteriological Techniques , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , Burkholderia/isolation & purification , Species Specificity , Bacterial Proteins/analysis , Algorithms , Reproducibility of Results , Burkholderia Infections/complications , Burkholderia Infections/microbiology , Burkholderia/classification , Burkholderia/chemistry , Cystic Fibrosis/complications , Cystic Fibrosis/microbiology
3.
Mycobiology ; : 105-111, 2019.
Article in English | WPRIM | ID: wpr-760521

ABSTRACT

Many of the fungicides and antibiotics currently available against plant pathogens are of limited use due to the emergence of resistant strains. In this study, we examined the effects of diphenyleneiodonium chloride (DPIC), an inhibitor of the superoxide producing enzyme NADPH oxidase, against fungal and bacterial plant pathogens. We found that DPIC inhibits fungal spore germination and bacterial cell proliferation. In addition, we demonstrated the potent antibacterial activity of DPIC using rice heads infected with the bacterial pathogen Burkholderia glumae which causes bacterial panicle blight (BPB). We found that treatment with DPIC reduced BPB when applied during the initial flowering stage of the rice heads. These results suggest that DPIC could serve as a new and useful antimicrobial agent in agriculture.


Subject(s)
Agriculture , Anti-Bacterial Agents , Burkholderia , Cell Proliferation , Flowers , Germination , Head , NADPH Oxidases , Plants , Spores, Fungal , Superoxides
4.
Pesqui. vet. bras ; 38(10): 1913-1917, out. 2018. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-976384

ABSTRACT

Amorimia septentrionalis is an important sodium monofluoroacetate (MFA) containing plant that causes sudden death in ruminants in northeastern Brazil. MFA degrading bacteria are being used in the prevention against poisoning by this plant. The aim of this study was to evaluate if goats which had per os received MFA degrading bacteria remained resistant when exposed to natural poisoning by A. septentrionalis. Eighteen goats were randomly distributed into three groups: the goats of Group 1 previously received, during 40 days, a solution containing the bacteria Ralstonia sp. and Burkholderia sp., those goats in the Group 2 received the bacteria Paenibacillus sp. and Cupriavidus sp. and goats from Group 3 did not receive any bacteria. After the administration period, during 60 days, the animals of all groups were released to graze on a one hectare paddock, with significant amount of A. septentrionalis. They were observed daily for the spontaneous consumption of A. septentrionalis leaves and the occurrence of clinical signs of poisoning or sudden death. Goats from all groups consumed significant amounts of A. septentrionalis during the experimental period. Goats that did not receive MFA-degrading bacteria (Group 3) became sick and died from the 25th to the 27th day of the experiment, whereas the goats of the groups that received MFA-degrading bacteria showed only clinical sings when A. septentrionalis regrowth after the 55th day of the experiment. The days elapsed from field observation to death of Group 3 goats (25.5±0.9 days) were significantly lower (p<0.05) than Group 1 (58.6±1.3 days) and Group 2 (57.8±1.5 days). Thus, it can be concluded that administration of MFA degrading bacteria increases the resistance to natural poisoning by A. septentrionalis.(AU)


Amorimia septentrionalis que contém monofluoroacetato de sódio (MFA) é responsável pela ocorrência de mortes súbitas em ruminantes no nordeste do Brasil. Bactérias degradadoras desse composto estão sendo utilizadas na prevenção contra a intoxicação por essa planta. O objetivo deste estudo foi avaliar se caprinos que receberam, via oral, bactérias degradadoras de MFA permaneciam resistentes quando expostos a intoxicação natural por A. septentrionalis. Dezoito caprinos foram divididos em três grupos, os caprinos do Grupo 1 receberam anteriormente, durante 40 dias, uma solução contendo as bactérias Ralstonia sp. e Burkholderia sp., os do Grupo 2 receberam, também por 40 dias as bactérias Paenibacillus sp. e Cupriavidus sp. e os do Grupo 3 não receberam nenhuma bactéria. Após o período de administração, durante 60 dias, os animais de todos os grupos foram soltos para pastar em um piquete de um hectare, que apresentava uma quantidade significativa da planta. Diariamente eles foram observados quanto ao consumo espontâneo das folhas de A. septentrionalis e quanto à presença de sinais clínicos de intoxicação ou morte. Os caprinos de todos os grupos consumiram quantidades significantes da planta durante o período experimental. Os caprinos que não receberam as bactérias degradantes de MFA (Grupo 3) adoeceram e morreram entre o 25º e o 27º dia de experimento, enquanto que os que receberam as bactérias degradantes de MFA (Grupo 1 e 2) só apresentaram sinais clínicos no 55º dia de experimento, o que coincidiu com a rebrota da planta. Os dias transcorridos desde a observação a campo até a morte dos caprinos do Grupo 3 (25,5±0,9 dias) foram significativamente menores (p<0,05) que os do Grupo 1 (58,6±1,3 dias) e do Grupo 2 (57,8±1,5 dias). Com isso pode-se concluir que a administração de bactérias degradadoras de MFA aumenta à resistência a intoxicação natural por A. septentrionalis.(AU)


Subject(s)
Animals , Plant Poisoning/therapy , Plant Poisoning/veterinary , Bacteria/enzymology , Ruminants , Malpighiaceae/poisoning , Fluoroacetates/antagonists & inhibitors , Burkholderia , Ralstonia , Cupriavidus , Paenibacillus
5.
Rev. argent. microbiol ; 49(4): 394-401, Dec. 2017. ilus, tab
Article in English | LILACS | ID: biblio-958021

ABSTRACT

Leguminous plants have received special interest for the diversity of β-proteobacteria in their nodules and are promising candidates for biotechnological applications. In this study, 15 bacterial strains were isolated from the nodules of the following legumes: Indigofera thibaudiana, Mimosa diplotricha, Mimosa albida, Mimosa pigra, and Mimosa pudica, collected in 9 areas of Chiapas, Mexico. The strains were grouped into four profiles of genomic fingerprints through BOX-PCR and identified based on their morphology, API 20NE biochemical tests, sequencing of the 16S rRNA, nifH and nodC genes as bacteria of the Burkholderia genus, genetically related to Burkholderia phenoliruptrix, Burkholderia phymatum, Burkholderia sabiae, and Burkholderia tuberum. The Burkholderia strains were grown under stress conditions with 4% NaCl, 45°C, and benzene presence at 0.1% as the sole carbon source. This is the first report on the isolation of these nodulating species of the Burkholderia genus in legumes in Mexico.


Las plantas leguminosas han recibido especial interés por la diversidad de β-proteobacteria que albergan en sus nodulos; algunas de estas bacterias son candidatas prometedoras para aplicaciones biotecnológicas. En el presente trabajo se aislaron 15 cepas bacterianas de los nodulos de las leguminosas Indigofera thibaudiana, Mimosa diplotricha, Mimosa albida, Mimosa pigra y Mimosa púdica, colectadas en 9 áreas de Chiapas, México. Las cepas fueron agrupadas en 4 perfiles de huellas genómicas por BOX-PCR e identificadas sobre la base de su morfología, pruebas bioquímicas API 20NE y secuenciación de los genes 16S ARNr, nifH y nodC como bacterias del género Burkholderia relacionadas genéticamente con Burkholderia phenoliruptrix, Burkholderia phymatum, Burkholderia sabiae y Burkholderia tuberum. Las cepas de Burkholderia crecieron en condiciones de estrés con NaCl al 4%, a una temperatura de 45°C y en presencia de benceno al 0,1% como única fuente de carbono. Este es el primer reporte del aislamiento de especies de Burkholderia nodulantes en leguminosas en México.


Subject(s)
Burkholderia , Fabaceae , Phylogeny , Stress, Physiological , Symbiosis , DNA, Bacterial , RNA, Ribosomal, 16S , Sequence Analysis, DNA , Burkholderia/isolation & purification , Burkholderia/genetics , Fabaceae/microbiology , Mexico
6.
Article in English | WPRIM | ID: wpr-219280

ABSTRACT

BACKGROUND: Musculoskeletal involvement in melioidosis is often seen in conjunction with a disseminated illness. Recent reports suggest that operative management of musculoskeletal melioidosis has favourable results. The purpose of this study was to review the patient profile and clinical outcomes of Burkholderia pseudomallei infection in the musculoskeletal system. METHODS: Hospital records of 163 patients who were diagnosed to have B. pseudomallei infection between January 2009 and December 2014 were reviewed. Patients underwent surgical and nonsurgical management depending upon the tissue of involvement. Epidata software was used to record the data. The SPSS ver. 17.0 was used for analysis. RESULTS: Eighteen out of 24 patients who had musculoskeletal melioidosis were available for follow-up. Septic arthritis, osteomyelitis, and intramuscular abscess were the common diagnosis, with 6 patients in each group. Twelve patients required surgical intervention. All patients received a full course of parenteral ceftazidime followed by oral doxycycline and co-trimoxazole. Two out of 6 patients (33.3%) died among those who had nonsurgical management as compared to none in the group who had surgical management. This was significant at 10% level of significance (p = 0.098). The rest were followed up for a minimum of 1 year with no evidence of disease recurrence. CONCLUSIONS: This series describing musculoskeletal involvement in melioidosis is the largest such study from a recently recognized ‘endemic’ region. Of importance are the patterns of musculoskeletal involvement, pitfalls in diagnosis and adequate clinical response with timely diagnosis and appropriate surgical management.


Subject(s)
Abscess , Arthritis, Infectious , Burkholderia pseudomallei , Burkholderia , Ceftazidime , Debridement , Diagnosis , Doxycycline , Follow-Up Studies , Hospital Records , Humans , Melioidosis , Musculoskeletal System , Osteomyelitis , Recurrence , Synovitis , Trimethoprim, Sulfamethoxazole Drug Combination
7.
Chinese Journal of Biotechnology ; (12): 912-926, 2016.
Article in Chinese | WPRIM | ID: wpr-242287

ABSTRACT

Production of chiral amines and unnatural amino-acid using ω-transaminase can be achieved by kinetic resolution and asymmetric synthesis, thus ω-transaminase is of great importance in the synthesis of pharmaceutical intermediates. By genomic data mining, a putative ω-transaminase gene hbp was found in Burkholderia phytofirmans PsJN. The gene was cloned and over-expressed in Escherichia coli BL21 (DE3). The recombinant enzyme (HBP) was purified by Ni-NTA column and its catalytic properties and substrate profile were studied. HBP showed high relative activity (33.80 U/mg) and enantioselectivity toward β-phenylalanine (β-Phe). The optimal reaction temperature and pH were 40 ℃ and 8.0-8.5, respectively. We also established a simpler and more effective method to detect the deamination reaction of β-Phe by UV absorption method using microplate reader, and demonstrated the thermodynamic property of this reaction. The substrate profiling showed that HBP was specific to β-Phe and its derivatives as the amino donor. HBP catalyzed the resolution of rac-β-Phe and its derivatives, the products (R)-amino acids were obtained with about 50% conversions and 99% ee.


Subject(s)
Bacterial Proteins , Genetics , Burkholderia , Cloning, Molecular , Escherichia coli , Genetics , Metabolism , Transaminases , Genetics
8.
Laboratory Medicine Online ; : 219-222, 2015.
Article in Korean | WPRIM | ID: wpr-128362

ABSTRACT

Melioidosis, which is an infectious disease caused by Burkholderia pseudomallei, is prevalent mostly in Southeast Asia and northern Australia; it can progress to abscess formation, pneumonia and sepsis, and ultimately cause death. A 66-yr-old male patient with diabetes mellitus was hospitalized for sepsis 3 months after coming back from Cambodia, and B. pseudomallei was identified from the blood culture. The B. pseudomallei strain was found to be susceptible to carbapenem, and non-susceptible to trimethoprim/sulfamethoxazole and ceftazidime. Although the patient was treated with carbapenem, to which the strain was susceptible, the bacteremia persisted, and progressed to septic shock and pneumonia, and eventually to acute respiratory distress syndrome (ARDS). The patient died on the 12th day of hospitalization. This study, which reports the first case of ceftazidime-nonsusceptible B. pseudomallei in Korea, indicates the importance of B. pseudomallei infection, which is highly likely to be imported to Korea, and discuss its clinical progress, which can lead to fatality.


Subject(s)
Abscess , Asia, Southeastern , Australia , Bacteremia , Burkholderia pseudomallei , Burkholderia , Cambodia , Ceftazidime , Communicable Diseases , Diabetes Mellitus , Hospitalization , Humans , Korea , Male , Melioidosis , Pneumonia , Respiratory Distress Syndrome , Sepsis , Shock, Septic
9.
Childhood Kidney Diseases ; : 143-147, 2015.
Article in English | WPRIM | ID: wpr-27107

ABSTRACT

PURPOSE: Burkholderia cepacia is an aerobic, glucose-non-fermenting, gramnegative bacillus that mainly affects immunocompromised and hospitalized patients. Burkholderia cepacia has high levels of resistance to many antimicrobial agents, and therapeutic options are limited. The authors sought to analyze the incidence, clinical manifestation, risk factors, antimicrobial sensitivity and outcomes of B. cepacia urinary tract infection (UTI) in pediatric patients. METHODS: Pediatric patients with urine culture-proven B. cepacia UTI between January 2000 and December 2014 at Samsung Medical Center, a tertiary referral hospital in Seoul, Republic of Korea, were included in a retrospective analysis of medical records. RESULTS: Over 14 years, 14 patients (male-to-female ratio of 1:1) were diagnosed with B. cepacia UTI. Of 14 patients with UTI, 11 patients were admitted to the intensive care unit, and a bladder catheter was present in 9 patients when urine culture was positive for B. cepacia. Patients had multiple predisposing factors for UTI, including double-J catheter insertion (14.2%), vesico-ureteral reflux (28. 6%), congenital heart disease (28.6%), or malignancy (21.4%). Burkholderia cepacia isolates were sensitive to piperacillin-tazobactam and sulfamethoxazole-trimethoprim, and resistant to amikacin and colistin. Treatment with parenteral or oral antimicrobial agents including piperacillin-tazobactam, ceftazidime, meropenem, and sulfamethoxazole-trimethoprim resulted in complete recovery from UTI. CONCLUSION: Burkholderia cepacia may be a causative pathogen for nosocomial UTI in pediatric patients with predisposing factors, and appropriate selection of antimicrobial therapy is necessary because of high levels of resistance to empirical therapy, including aminoglycosides.


Subject(s)
Amikacin , Aminoglycosides , Anti-Infective Agents , Bacillus , Burkholderia cepacia , Burkholderia , Catheters , Causality , Ceftazidime , Child , Colistin , Heart Defects, Congenital , Humans , Incidence , Intensive Care Units , Medical Records , Republic of Korea , Retrospective Studies , Risk Factors , Seoul , Tertiary Care Centers , Urinary Bladder , Urinary Tract Infections , Urinary Tract , Vesico-Ureteral Reflux
10.
Biosci. j. (Online) ; 30(3): 655-665, may/june 2014. tab, ilus
Article in Portuguese | LILACS | ID: biblio-947180

ABSTRACT

Este trabalho objetivou avaliar o efeito da inoculação de seis estirpes de bactérias promotoras de crescimento de plantas (BPCP), Burkholderia spp. (B7 e B16), Pseudomonas spp. (P18 e P21) e Rhizobium spp. (R65 e R82) e duas misturas contendo uma estirpe de cada gênero no crescimento de seis cultivares de arroz (BRS Bonança, BRS Aimoré, BRS Talento, BRSMG Curinga, Japonês e Bolinha). As características fisiológicas das bactérias foram determinadas em ensaios in vitro. Foi conduzido um experimento sob condições de casa de vegetação onde as BPCP foram inoculadas em cultivares de arroz, cultivados em vasos contendo 1 kg de solo, em delineamento de blocos casualizados com 4 repetições. Aos 60 dias após a emergência foram avaliados parâmetros de crescimentos relacionados à parte aérea e ao sistema radicular dos cultivares de arroz. Entre as bactérias, somente duas (B7 e R65) não apresentaram capacidade de produção in vitro de auxina. Todas as estirpes apresentaram atividade de catalase e formação de película em meio NFb semi-sólido sem N ou presença de nódulo em feijoeiro, indicando capacidade de fixação biológica de nitrogênio. A inoculação das BPCP em arroz proporcionou aumentos médios de 10% em relação ao controle para a área foliar e massa seca da parte aérea e de 9% para o comprimento e massa seca de raiz. Entre os cultivares, BRS Aimoré apresentou maior interação com as estirpes estudadas e a estirpe P21 de Pseudomonas spp. proporcionou diferença significativa em relação ao controle em parâmetros relacionados à parte aérea e ao sistema radicular.


Aiming to evaluate the effect of the inoculation of six strains of plant growth-promoting bacteria (PGPBs) Burkholderia spp. (B7 and B16), Pseudomonas spp. (P18 and P21) and Rhizobium spp. (R65 and R82) and two mixtures containing one strain of each genera on the growth of six rice cultivars (BRS Bonança, BRS Aimoré, BRS Talento, BRSMG Curinga, Japonês and Bolinha). Physiological characteristics of the bacteria were determined on in vitro assays. Rice cultivars were inoculated with PGPB under greenhouse conditions and cultivated on 1 kg pots filled with soil on a randomized block design with four replicates. At 60 days after emergence growth parameters related with the shoot and root were evaluated for rice cultivars. Among the evaluated bacteria only two (B7 and R65) were not able to produce auxin under in vitro conditions. All strains showed catalase activity and film formation in semisolid medium NFb without N or nodulation in common bean, indicating the ability of biological nitrogen fixation. Inoculation of PGPBs in rice provided increases of 10% as compared to the control regarding leaf area and dry mass of shoots and 9% for length and root dry weight. Among the cultivars BRS Aimoré showed greater interaction with the strains studied and the strain P21 of Pseudomonas spp. promoted significant difference compared to the control for the parameters related with the shoot and root system.


Subject(s)
Pseudomonas , Rhizobium , Oryza/growth & development , Burkholderia , Genotype
11.
Med. lab ; 19(9-10): 465-472, 2013. ilus, tab
Article in Spanish | LILACS | ID: biblio-834764

ABSTRACT

Resumen: La melioidosis es una enfermedad infecciosa causada por una bacteria gram-negativa intracelularBurkholderia pseudomallei. Este microorganismo es un saprofito ambiental en regiones endémicas,algunas de ellas ubicadas probablemente en el territorio nacional y presenta alto riesgo de propagación como epidemia en zonas no endémicas. La melioidosis es una enfermedad clínicamente diversa, la mayoría de las infecciones son asintomáticas; sin embargo, si el paciente es sintomático, se puede clasificar como aguda o crónica según su persistencia. La presentación clínica más común es la afectación pulmonar y al diagnóstico predominan baciloscopias persistentemente negativas. Aquí se presenta un caso de un paciente con tos crónica, expectoración mucopurulenta, sudoración nocturna y disnea.


Abstract: Melioidosis is an infectious disease caused by the intracellular gram-negative bacterium Burkholderia pseudomallei. This microorganism is an environmental saprophyte in endemic regions, some of which are likely located in our country and have a high risk of spreading to non-endemic areas. Melioidosis is a clinically diverse disease. Most infections are asymptomatic; however, if symptoms are present, the disease can be classified as acute or chronic according to persistence of symptoms. In addition, lung involvement is the most common clinical presentation and one of the main diagnostic features is consistently negative bacilloscopy. Here we present a case report of a patient with chronic cough, mucopurulent expectoration, night sweats and dyspnea.


Subject(s)
Humans , Burkholderia , Burkholderia Infections , Burkholderia pseudomallei , Melioidosis
12.
Article in Korean | WPRIM | ID: wpr-653534

ABSTRACT

Burkholderia cepacia is a highly virulent pathogen known to cause opportunistic infections in immunocompromised patients. It accelerates lung disease and causes necrotizing pneumonia with associated severe sepsis, known as cepacia syndrome. In particular, lung transplant recipients infected with Burkholderia cepacia show higher mortality after lung transplantation than those who are not infected with this organism. Due to broad-spectrum antibiotic resistance, a combination therapy should be used according to the results of the susceptibility test. This bacterial infection is rare in Korea, and no case was reported in lung transplant recipients. However, we report a case of pneumonia caused by Burkholderia cepacia after lung transplantation. As Burkholderia cepacia was grown from a sputum culture, the patient was treated initially with a combination of meropenem and trimethoprim/sulfamethoxazole and then ceftazidime and trimethoprim/sulfamethoxazole as a result of leukopenia. After antibiotics treatment for 20 days, sputum cultures became negative for Burkholderia cepacia and the patient successfully recovered.


Subject(s)
Anti-Bacterial Agents , Bacterial Infections , Burkholderia , Burkholderia cepacia , Ceftazidime , Cystic Fibrosis , Drug Resistance, Microbial , Humans , Immunocompromised Host , Korea , Leukopenia , Lung , Lung Diseases , Lung Transplantation , Opportunistic Infections , Pneumonia , Sepsis , Sputum , Thienamycins
13.
Biosci. j. (Online) ; 28(Supplement 1 - XXXIII Congresso Brasileiro de Ciência do Solo): 112-121, 2012.
Article in Portuguese | LILACS | ID: biblio-912166

ABSTRACT

A cana-de-açúcar é uma fonte importante de alimento e bioenergia. Assim, o estudo da interação com bactérias endofíticas que fixam o nitrogênio atmosférico (FBN) e produzem o ácido indol acético (AIA) vem sendo amplamente explorado. Porém, devido à sensibilidade bacteriana, tanto a produção de AIA, como a FBN, podem sofrer o impacto de fatores edafoclimáticos. O objetivo deste trabalho foi avaliar a influência de diferentes concentrações de NaCl sobre o crescimento bacteriano, a FBN e a produção de AIA in vitro de linhagens bacterianas do gênero Burkholderia, isoladas endofiticamente de plantas de cana-de-açúcar, cultivadas no estado de Pernambuco. Para tanto, duas linhagens bacterianas endofíticas das espécies Burkholderia gladioli e B. heleia foram inoculadas em meio TSA (com L-triptofano) acrescido de cinco concentrações de NaCl: 0,1; 1; 10; 25 e 50 g.L-1 e incubadas sob agitação. O crescimento bacteriano e a produção de AIA foram avaliados através de um espectrofotômetro por 48 horas. Para o teste de fixação de nitrogênio, as linhagens foram inoculadas em meio LGI-P semi-sólido acrescido de cinco concentrações de NaCl: 0,1; 1; 10; 25 e 50 g.L1 e incubadas a 28ºC por 10 dias. Foi possível observar que altas concentrações de NaCl influenciaram negativamente o crescimento bacteriano e a produção do AIA in vitro por B. glaidoli e B. heleia. E que, concentrações de NaCl acima de 25 g.L-1 inibiram a capacidade de fixar nitrogênio, de ambas as linhagens.


The sugarcane is an important source of food and bioenergy. So, the study of its interaction with endophytic bacteria that fix atmospheric nitrogen (BNF) and produce indole acetic acid (IAA) has been widely explored. However, due to bacterial sensitivity, both the production of IAA as the BNF may suffer the impact of soil and climatic factors. The goal of this study was to evaluate the influence of the NaCl different concentrations on the bacterial growth, on the BNF and on the IAA production in vitro by endophytic bacterial strains of the genus Burkholderia isolated from sugarcane plants grown in the Pernambuco State. In this study, two endophytic bacterial strains of Burkholderia gladioli and B. heleia species were inoculated in TSA (with L-tryptophan) added of five NaCl concentrations: 0.1, 1, 10, 25 and 50 g.L-1 and incubated under agitation. Bacterial growth and IAA production were assessed using a spectrophotometer for 48 hours. The nitrogen fixation test was carried out by the inoculation of the bacteria in semi-solid LGP-I added of five NaCl concentrations: 0.1, 1, 10, 25 and 50 g.L-1 and incubated at 28°C during 10 days. It was observed that high concentrations of NaCl influenced negatively the bacterial growth and indole acetic acid production in vitro by B. glaidoli and B. heleia. Nevertheless, NaCl concentrations above 25 g.L-1 inhibited the ability to fix nitrogen in both strains.


Subject(s)
Sodium Chloride , Saltpetre Soils , Burkholderia , Saccharum , Burkholderia gladioli , Nitrogen Fixation , Biofuels
14.
Genomics & Informatics ; : 249-255, 2012.
Article in English | WPRIM | ID: wpr-11756

ABSTRACT

In this study, fosmid cloning strategies were used to assess the microbial populations in water from the International Space Station (ISS) drinking water system (henceforth referred to as Prebiocide and Tank A water samples). The goals of this study were: to compare the sensitivity of the fosmid cloning strategy with that of traditional culture-based and 16S rRNA-based approaches and to detect the widest possible spectrum of microbial populations during the water purification process. Initially, microbes could not be cultivated, and conventional PCR failed to amplify 16S rDNA fragments from these low biomass samples. Therefore, randomly primed rolling-circle amplification was used to amplify any DNA that might be present in the samples, followed by size selection by using pulsed-field gel electrophoresis. The amplified high-molecular-weight DNA from both samples was cloned into fosmid vectors. Several hundred clones were randomly selected for sequencing, followed by Blastn/Blastx searches. Sequences encoding specific genes from Burkholderia, a species abundant in the soil and groundwater, were found in both samples. Bradyrhizobium and Mesorhizobium, which belong to rhizobia, a large community of nitrogen fixers often found in association with plant roots, were present in the Prebiocide samples. Ralstonia, which is prevalent in soils with a high heavy metal content, was detected in the Tank A samples. The detection of many unidentified sequences suggests the presence of potentially novel microbial fingerprints. The bacterial diversity detected in this pilot study using a fosmid vector approach was higher than that detected by conventional 16S rRNA gene sequencing.


Subject(s)
Biomass , Bradyrhizobium , Burkholderia , Clone Cells , Cloning, Organism , Dermatoglyphics , DNA , DNA, Ribosomal , Drinking , Drinking Water , Electrophoresis, Gel, Pulsed-Field , Genes, rRNA , Groundwater , Mesorhizobium , Nitrogen , Pilot Projects , Plant Roots , Polymerase Chain Reaction , Ralstonia , Soil , Water , Water Purification
15.
Korean Journal of Medicine ; : 526-532, 2011.
Article in Korean | WPRIM | ID: wpr-164060

ABSTRACT

Melioidosis is an infectious disease caused by the Gram-negative bacillus, Burkholderia pseudomallei. It is endemic in Southeast Asia and northern Australia, where B. pseudomallei is a soil saprophyte and invades the host through inoculation, inhalation or ingestion. It infects mostly adults with predisposing conditions, mainly diabetes mellitus. The lung is the most commonly affected organ, presenting with either cough or fever resulting from a primary spread. Also, seeding and abscess formation can arise in any organ. However, prostatic abscess due to melioidosis is uncommon. We report a case of pneumonia, and prostate and scrotal abscesses due to Burkholderia pseudomallei, in a 48-year-old man with diabetes mellitus who had a history of working in Malaysia. This is the first report of genitourinary infection of melioidosis in Korea.


Subject(s)
Abscess , Adult , Asia, Southeastern , Australia , Bacillus , Burkholderia , Burkholderia pseudomallei , Communicable Diseases , Cough , Diabetes Mellitus , Eating , Fever , Humans , Inhalation , Korea , Lung , Malaysia , Melioidosis , Middle Aged , Pneumonia , Prostate , Seeds , Soil
16.
Infection and Chemotherapy ; : 490-493, 2011.
Article in Korean | WPRIM | ID: wpr-173961

ABSTRACT

Burkholderia cepacia is an aerobic gram-negative rod that causes healthcare-associated infections through contaminated disinfectants, fluids, and medical equipment. This bacterium shows resistance to various antibiotics. For this reason, available therapeutic antimicrobial agents are limited, and the optimal antimicrobial therapeutic regimen was still not well-determined in the case of infective spondylitis. We experienced a case of B. cepacia bacteremia with infective spondylitis and paraspinal abscesses which did not respond to carbapenem monotherapy, but was did respond to ceftazidime and sulfamethoxzaole/trimethoprim combination therapy.


Subject(s)
Abscess , Anti-Bacterial Agents , Anti-Infective Agents , Bacteremia , Burkholderia , Burkholderia cepacia , Ceftazidime , Disinfectants , Spondylitis , Trimethoprim, Sulfamethoxazole Drug Combination
17.
An. acad. bras. ciênc ; 80(4): 749-761, Dec. 2008. ilus, graf, tab
Article in English | LILACS | ID: lil-497117

ABSTRACT

This work aimed to evaluate density of associative diazotrophic bacteria populations in soil and grass root samples from heavy metal contaminated sites, and to characterize isolates from these populations, both, phenotypically (Zinc, Cadmium and NaCl tolerance in vitro, and protein profiles) and genotypically (16S rDNA sequencing), as compared to type strains of known diazotrophic species. Densities were evaluated by using NFb, Fam and JNFb media, commonly used for enrichment cultures of diazotrophic bacteria. Bacterial densities found in soil and grass root samples from contaminated sites were similar to those reported for agricultural soils. Azospirillum spp. isolates from contaminated sites and type strains from non-contaminated sites varied substantially in their in vitro tolerance to Zn+2 and Cd+2, being Cd+2 more toxic than Zn+2. Among the most tolerant isolates (UFLA 1S, 1R, S181, S34 and S22), some (1R, S34 and S22) were more tolerant to heavy metals than rhizobia from tropical and temperate soils. The majority of the isolates tolerant to heavy metals were also tolerant to salt stress as indicated by their ability to grow in solid medium supplemented with 30 g L-1 NaCl. Five isolates exhibited high dissimilarity in protein profiles, and the 16S rDNA sequence analysis of two of them revealed new sequences for Azospirillum.


Objetivou-se avaliar a densidade de populações de bactérias diazotróficas associativas em amostras de solos e de raízes de gramíneas oriundas de sítios contaminados com metais pesados, e caracterizar isolados destas populações através da análise fenotípica (tolerância aos metais pesados zinco e cádmio e à NaCl in vitro, perfis protéicos), e genotípica (seqüenciamento de 16S rDNA), comparados às estirpes tipo das mesmas espécies. As densidades foram avaliadas nos meios NFb, Fam e LGI, comumente utilizados para culturas de enriquecimento de populações de bactérias diazotróficas associativas. As densidades encontradas em amostras de solo e raiz de sítios contaminados foram semelhantes àquelas relatadas na literatura para solos agrícolas. Isolados de Azospirillum spp. de solos contaminados e estirpes tipo oriundas de solos não contaminados variaram substancialmente com relação à tolerância a Zn+2 e Cd+2, sendo que Cd+2 mais tóxico que Zn+2. Dentre os isolados mais tolerantes (UFLA 1S, 1R, S181, S34, e S22), alguns(1R, S34 e S22) foram mais tolerantes a metais pesados que rizóbios isolados de solos de áreas tropicais e temperadas. A maioria dos isolados mais tolerantes a metais pesados também foi tolerante ao estresse salino, o que foi indicado por seu crescimento em meio sólido suplementado com 30 g L-1 de NaCl in vitro. Cinco isolados apresentaram alta dissimilaridade em perfis protéicos e o seqüenciamento do gene 16S rDNA em dois deles revelou que apresentam novas seqüências de Azospirillum.


Subject(s)
Azospirillum/drug effects , Burkholderia/drug effects , Herbaspirillum/drug effects , Metals, Heavy/toxicity , Plant Roots/microbiology , Poaceae/microbiology , Soil Microbiology , Azospirillum/genetics , Azospirillum/growth & development , Burkholderia/genetics , Burkholderia/growth & development , Colony Count, Microbial , DNA, Bacterial/genetics , Drug Resistance, Bacterial/genetics , Genotype , Herbaspirillum/genetics , Herbaspirillum/growth & development , Metals, Heavy/analysis , Phenotype , /genetics
18.
An. acad. bras. ciênc ; 80(3): 477-493, Sept. 2008. ilus, graf
Article in English | LILACS | ID: lil-491836

ABSTRACT

Burkholderia kururiensis is a diazotrophic bacterium originally isolated from a polluted aquifer environment and presents a high level of similarity with the rice endophyte "B. brasilensis" species. This work assessed the ability of B. kururiensis to endophytically colonize rice plantlets by monitoring different tissues of root-inoculated plants for the presence of bacterial growth in different media, electron microscopy and by 16S rDNA analysis. Observations of roots, stems and leaves of inoculated rice plantlets by electron microscopy revealed B. kururiensis colonization predominantly on root hair zones, demonstrating endophytic colonization primarily through the endodermis, followed by spreading into xylem vessels, a possible pathway leading to aerial parts. Although indifferent for the bacterial growth itself, addition of a nitrogen source was a limiting factor for endophytic colonization. As endophytic colonization was directly associated to an enhanced plant development, production of phytohormone auxin/indole-3-acetic acid by B. kururiensis was assayed with transgenic rice plantlets containing an auxin-responsive reporter (DR5-GUS). Our findings suggest the ability of auxin production by plant-associated B. kururiensis which may have a stimulatory effect on plant development, as evidenced by activation of DR5-GUS. We hereby demonstrate, for the first time, the ability of B. kururiensis to endophytically colonize rice, promoting both plant growth and rice grain yield.


Burkholderia kururiensis é uma bactéria diazotrófica, originalmente isolada de um ambiente aquático poluído e apresenta alto nível de similaridade com a espécie endofítica "B. brasilensis" encontrada na planta de arroz. Este artigo demonstrou a habilidade de B. kururiensis colonizar endofiticamente plântulas de arroz, após esta bactéria ter sido inoculada na raiz das plantas. Esta capacidade foi confirmada pelo crescimento bacteriano em diferentes tecidos da planta, por microscopia eletrônica e pela análise do 16S rADN. Observação por microscopia eletrônica das raízes, caule e folhas das plântulas de arroz inoculadas, revelou predominância da colonização de B. kururiensis na zona pilífera da raiz, demonstrando que a colonização endofítica inicia-se na endoderme, espalha-se pelo xilema, sendo esta a possível via para a bactéria alcançar as partes aéreas. A adição de uma fonte de nitrogênio, embora não tenha influenciado no crescimento bacteriano, foi um fator limitante para a colonização endofítica. Como a colonização endofítica mostrou-se diretamente associada ao aumento no desenvolvimento da planta, a produção do fitohormônio auxina/ácido 3-indolacético pela B. kururiensis foi verificada utilizando uma plântula de arroz transgênica, contendo o repórter responsivo para auxina (DR5-GUS). Nossos resultados sugerem que a produção de auxina pela B. kururiensis é responsável pelo estímulo no desenvolvimento da planta verificado pela ativação do DR5-GUS. Neste trabalho demonstramos, pela primeira vez, a habilidade de B. kururiensis colonizar endofiticamente a planta de arroz, promovendo tanto o aumento no crescimento da planta como a produção de sementes de arroz.


Subject(s)
Burkholderia/physiology , Indoleacetic Acids/analysis , Oryza/microbiology , Burkholderia/ultrastructure , Colony Count, Microbial , Microscopy, Electron , Oryza/growth & development , Polymerase Chain Reaction
19.
Infection and Chemotherapy ; : 233-236, 2008.
Article in English | WPRIM | ID: wpr-721687

ABSTRACT

Burkholderia cepacia is an important pathogen of humans in both immunocompromised or hospitalized patients. This aerobic gram-negative rod causes various clinically significant infections such as pneumonia, bacteremia, urinary tract infection, peritonitis, endophthalmitis, and keratitis. This uncommon pathogen is mainly associated with cystic fibrosis, dialysis, and immunocompromised states. We observed a 73-year-old woman without any underlying diseases, who had both lower leg pain and fever. She was later found to have spondylitis with epidural abscess at L5-S1. Culture of the epidural abscess revealed the growth of B. cepacia. To the best of our knowledge, this is the first case of pyogenic spondylitis caused by a rare infection of B. cepacia to be reported in Korea


Subject(s)
Adult , Aged , Bacteremia , Burkholderia , Burkholderia cepacia , Cystic Fibrosis , Dialysis , Endophthalmitis , Epidural Abscess , Female , Fever , Humans , Keratitis , Korea , Leg , Peritonitis , Pneumonia , Spondylitis , Urinary Tract Infections
20.
Infection and Chemotherapy ; : 233-236, 2008.
Article in English | WPRIM | ID: wpr-722192

ABSTRACT

Burkholderia cepacia is an important pathogen of humans in both immunocompromised or hospitalized patients. This aerobic gram-negative rod causes various clinically significant infections such as pneumonia, bacteremia, urinary tract infection, peritonitis, endophthalmitis, and keratitis. This uncommon pathogen is mainly associated with cystic fibrosis, dialysis, and immunocompromised states. We observed a 73-year-old woman without any underlying diseases, who had both lower leg pain and fever. She was later found to have spondylitis with epidural abscess at L5-S1. Culture of the epidural abscess revealed the growth of B. cepacia. To the best of our knowledge, this is the first case of pyogenic spondylitis caused by a rare infection of B. cepacia to be reported in Korea


Subject(s)
Adult , Aged , Bacteremia , Burkholderia , Burkholderia cepacia , Cystic Fibrosis , Dialysis , Endophthalmitis , Epidural Abscess , Female , Fever , Humans , Keratitis , Korea , Leg , Peritonitis , Pneumonia , Spondylitis , Urinary Tract Infections
SELECTION OF CITATIONS
SEARCH DETAIL