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1.
Article in Chinese | WPRIM | ID: wpr-360019

ABSTRACT

<p><b>OBJECTIVE</b>To summarize the clinical features and therapy experience of a case of CD5 positive diffuse large B cell lymphoma (CD5+ DLBCL) with autoimmune hemolytic anemia (AIHA).</p><p><b>METHODS</b>A 49-years old patient was investigated. The routine blood examination, bone marrow smear, Coombs test, serological test, chest CT, abdominal MR and immunochemistry etc were performed for this patient; and therapeutic effects of the chemotherapy regimen consisting of rituximab plus autologous hematopoietic stem cell transplantation (auto-HSCT) were observed.</p><p><b>RESULTS</b>The cervical lymphnode biopsy confirmed CD5+ DLBCL; the severe anemia, reticulocyte increase, Coombs test positive, and erythroid hyperplasia in bone marrow all suggested the occurence of autoimmune hemolytic anemia (AIHA). After plasma exchange, immune suppression using methylprednisolone, blood transfusion, one course of chemotherapy with "R-CHOP-E", the symptoms of AIHA in patient disappeared. After a continuous treatment for 3 courses of "R-CHOP-E", the bone marrow infiltration appeared, which was assessed as "PD", then the treatment was changed to the "R-ESHAP" for 4 courses, the patient was reassessed as "CR". The patient subsequently underwent auto-HSCT, followed up for 6 months, patientis still "CR".</p><p><b>CONCLUSION</b>The status of the CD5+ DLBCL patient with AIHA is severe, and the prognosis is poor. The curative effect of the chemotherapy regimen with rituximab plus auto-HSCT for this patien is well.</p>


Subject(s)
Anemia, Hemolytic, Autoimmune , Therapeutics , Antibodies, Monoclonal, Murine-Derived , Therapeutic Uses , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , CD5 Antigens , Metabolism , Cisplatin , Therapeutic Uses , Cyclophosphamide , Therapeutic Uses , Cytarabine , Therapeutic Uses , Doxorubicin , Therapeutic Uses , Etoposide , Therapeutic Uses , Hematopoietic Stem Cell Transplantation , Humans , Lymphoma, Large B-Cell, Diffuse , Therapeutics , Methylprednisolone , Therapeutic Uses , Middle Aged , Prednisone , Therapeutic Uses , Rituximab , Therapeutic Uses , Sentinel Lymph Node Biopsy , Vincristine , Therapeutic Uses
2.
Article in Chinese | WPRIM | ID: wpr-815027

ABSTRACT

OBJECTIVE@#To study the pathologic characteristics of bone marrow for CD5 positive small B cell lymphoma (SBL).
@*METHODS@#The pathologic profiles of 92 patients with CD5 positive SBL were retrospectively analyzed. The morphologic and immunophenotypic features were analyzed by flow cytometry and immunohistochemistry. IgH/CCND1 was examined by fluorescence in situ hybridization (FISH).
@*RESULTS@#A total of 92 patients with CD5 positive SBL were enrolled in this study, including 56 (60.9%) chronic lymphocytic leukemia /small lymphocytic lymphoma (CLL/SLL), 23 (25.0%) mantle cell lymphoma (MCL) and 13 other SBL (14.1%). Among the 13 other cases, 5, 4 and 4 cases were follicular lymphoma (FL), lymphoplasmacytic lymphoma (LPL) and splenic marginal zone lymphoma (SMZL), respectively. The frequency of patterns for bone marrow infiltration was as follow: diffuse pattern (19/92), mixed pattern (15/92), nodular pattern (9/92), interstitial pattern (8/92), and intrasinusodial pattern (2/92). All patients expressed CD19, CD20 and CD5. According to the immunophenotypic score system, all the CLL patients had 4-5 scores, while SMCL and other SBL patients had less than 3 scores. For the other SBL patients, 5 FL expressed CD10, while 3 FL, 1 LPL and 3 SMZL expressed CD23. There was a significant difference in the expression of CD23, sIgM, FMC7, CD11C and CD22 between the CLL and MCL groups (P<0.01). All 23 MCL patients expressed cyclin D1 and showed IgH/CCND1 gene translocation by FISH detection.
@*CONCLUSION@#CD5 positive SBL includes a variety of types of lymphoma. Patterns of bone marrow for CD5 positive SBL are diversity. Immunophenotypic analysis by flow cytometry is essential in the diagnosis and differential diagnosis of CD5 positive SBL, especially for CLL.


Subject(s)
Bone Marrow , Pathology , CD5 Antigens , Metabolism , Diagnosis, Differential , Flow Cytometry , Humans , Immunohistochemistry , In Situ Hybridization, Fluorescence , Leukemia, Lymphocytic, Chronic, B-Cell , Diagnosis , Lymphoma, B-Cell , Diagnosis , Lymphoma, Follicular , Diagnosis , Lymphoma, Mantle-Cell , Diagnosis , Oncogene Proteins, Fusion , Metabolism , Retrospective Studies , Splenic Neoplasms , Diagnosis
3.
Protein & Cell ; (12): 872-881, 2013.
Article in English | WPRIM | ID: wpr-757545

ABSTRACT

Calreticulin (CRT) is a multifunctional molecule in both intracellular and extracellular environment. We have previously found that a recombinant CRT fragment (rCRT/39-272) could modulate T cell-mediated immunity in mice via activation and expansion of CD1d(hi)CD5⁺ B cells as well as induction of CRT-specific regulatory antibodies. Antibody secreting cells (ASCs) are terminally differentiated B cells responsible for producing antibodies to participate in positive immune response as well as immune regulation. In this study, we demonstrate that rCRT/39-272 differentiates murine CD1d(hi)CD5⁺ B cells into ASCs marked by increased expression of plasma cell-associated transcription factors and production of polyreactive antibodies against DNA and CRT in vitro. Intraperitoneal administration of rCRT/39-272 augmented differentiation of CD1d(hi)CD5⁺ B cells into ASCs in naïve mice or mice with experimental autoimmune encephalomyelitis. Thus, we propose that ASC differentiation and subsequent antibody production of CD1d(hi)CD5⁺ B cells are key steps in CRT-mediated immunoregulation on inflammatory T cell responses.


Subject(s)
Animals , Antigens, CD1d , Metabolism , Autoantibodies , B-Lymphocytes , Cell Biology , Allergy and Immunology , Metabolism , CD5 Antigens , Metabolism , Calreticulin , Chemistry , Cell Differentiation , Encephalomyelitis, Autoimmune, Experimental , Allergy and Immunology , Humans , Mice , Peptide Fragments , Chemistry , Pharmacology , Solubility
4.
Article in Chinese | WPRIM | ID: wpr-332771

ABSTRACT

To explore the clinical characteristics, diagnosis, treatment outcome and prognosis of de novo CD5 positive diffuse large B cell lymphoma (CD5(+)DLBCL), clinical data of 10 patients with pathologically confirmed CD5(+)DLBCL were retrospectively analyzed. The results indicated that 9 out of 10 patients were older than 60 years. All cases were in III/IV stages according to Ann-Arbor Staging System. Bone marrow biopsy with immunohistochemistry showed lymphoma involvement in 5 cases. Nine patients received chemotherapy with anti-CD20 monoclonal antibody (Rituximab) except one. Five cases achieved CR, two cases achieved PR, two cases achieved SD, one case achieved PD. Eight cases died within 2 years because of relapse or disease progression, in which 3 cases developed central nervous system lymphoma. The median survival time was 16 (1-23) months, 2-year survival rate was 20.40%. It is concluded that de novo CD5(+) DLBCL is rare in clinic, but it is a kind of highly aggressive lymphoma with poor prognosis. So, new treatment strategy should be explored.


Subject(s)
Aged , Aged, 80 and over , Antibodies, Monoclonal, Murine-Derived , Therapeutic Uses , CD5 Antigens , Metabolism , Female , Humans , Lymphoma, Large B-Cell, Diffuse , Drug Therapy , Metabolism , Pathology , Male , Middle Aged , Neoplasm Staging , Prognosis , Retrospective Studies , Rituximab , Survival Rate , Treatment Outcome
5.
Article in English | WPRIM | ID: wpr-169073

ABSTRACT

Primary gastric lymphoma is a rare gastric malignancy. Its diagnostic process is complex. Clinician may find initial diagnosis of primary gastric lymphoma unreliable, especially when it indicates the rarest subtype of gastric lymphoma, while its initial endoscopic presentation fails to raise the slightest suspicion of primary gastric lymphoma. A 53-year-old Korean man was diagnosed, by endoscopic examination, with a round submucosal tumor of the stomach. Deep endoscopic biopsy, however, confirmed CD5 positive gastric lymphoma. Surgical treatment was performed for diagnosis and treatment. Postoperative histological examination confirmed gastric schwannoma. Gastric schwannoma is a spindle cell tumor, characterized by a peripheral cuff-like lymphocytic infiltration. Deep endoscopic biopsy may have been misdirected to the peripheral lymphoid cuff, failing to acquire spindle cells. The literature has been reviewed, and options for diagnostic accuracy have been suggested.


Subject(s)
Antigens, CD20/metabolism , CD5 Antigens/metabolism , Diagnosis, Differential , Gastric Mucosa/metabolism , Gastroscopy , Humans , Male , Middle Aged , Neurilemmoma/diagnosis , Stomach Neoplasms/diagnosis , Tomography, X-Ray Computed
6.
Chinese Journal of Hematology ; (12): 1028-1032, 2012.
Article in Chinese | WPRIM | ID: wpr-323498

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the number of peripheral blood CD5(+) B cells and their ability of secreting IL-10 in patients with immune thrombocytopenia (ITP).</p><p><b>METHODS</b>Peripheral blood lymphocytes were isolated from 57 pre-treated, 40 post-treated ITP patients and 25 controls using Ficoll-Hypaque density centrifugation and then stained with PE-CD5/FITC-CD19 for flow cytometric analysis. After 24-hour culture, lymphocytes were stained with APC-IL-10 for intracellular cytokine detection. ELISA assay was employed to determine IL-10 concentration in supernatants.</p><p><b>RESULTS</b>The percentage and absolute number of CD5(+) B cells in peripheral blood from pre-treated ITP patients were significantly higher than that from normal controls (3.75 ± 2.37)% vs (2.10 ± 1.08)%, P < 0.01; (6.29 ± 5.77)× 10(7)/L vs (3.06 ± 1.90)× 10(7)/L, P < 0.01. CD5(+) B cells expressed more intracellular IL-10 than other lymphocyte subsets both in ITP patients and normal controls. The percentages of IL-10(+) cells within CD5(+) B cells in pre-treated ITP patients and normal controls were (29.51 ± 20.73)% and(15.90 ± 9.58)%, respectively(P < 0.01). Intracellular mean fluorescence intensity (MFI) of IL-10 in CD5(+) B cells was 27.95 ± 13.99 in pre-treated patients, which was significantly higher than that in controls (P < 0.01). In contrast, IL-10 concentration in supernatants was (173.05 ± 102.50) ng/L in pre-treated ITP group, which was lower than that (230.61 ± 76.96) ng/L in controls. In patients who achieved remission, the number of CD5(+) B cells decreased to level comparable to normal controls. While intracellular IL-10 MFI of CD5(+) B cells in post-treated ITP patients remained as high as in pre-treated ones, the IL-10 concentration in supernatants increased to level similar to controls.</p><p><b>CONCLUSION</b>The significantly increased number of CD5(+) B cells and accumulated IL-10 in CD5(+) B cells suggested impaired IL-10 secretion in ITP patients. The number and the ability of secreting IL-10 of CD5(+) B cells could be restored after effective treatments in patients with ITP.</p>


Subject(s)
Adult , Aged , B-Lymphocytes , Allergy and Immunology , Metabolism , CD5 Antigens , Metabolism , Case-Control Studies , Female , Humans , Interleukin-10 , Blood , Male , Middle Aged , Purpura, Thrombocytopenic, Idiopathic , Blood , Allergy and Immunology , Young Adult
7.
Chinese Journal of Pathology ; (12): 156-160, 2012.
Article in Chinese | WPRIM | ID: wpr-241967

ABSTRACT

<p><b>OBJECTIVE</b>To analyze CD5 expression in diffuse large B cell lymphoma (DLBCL) and to explore its relationship with the clinicopathological characteristics.</p><p><b>METHODS</b>The clinical data from 160 DLBCL patients who were treated in First Bethune Hospital of Jilin University from January 2001 to December 2010 were retrospectively analyzed. Immunohistochemical staining (SP method) for CD5, CD10, bcl-6 and MUM-1 was performed on the paraffin-embedded tissue. The relationship between CD5 expression and the clinicopathological characteristics was evaluated by Chi-square test. Survival analysis adopted Kaplan-Meier analysis and Log-rank test.</p><p><b>RESULTS</b>In the patients aged 60 years or older, the incidence of CD5(+) lymphoma (12/17) was significantly higher than that of CD5(-) ones (39.9%, 57/143); two or more extranodal involvements in CD5(+) patients (11/17) were more commonly found than that of CD5(-)patients (31.5%, 45/143); DLBCL-related death in CD5(+) patients (13/17) was higher than that of CD5(-) patients (37.1%, 53/143). Survival analysis showed that the overall survival (OS) and the event-free survival (EFS) of CD5(+) patients were significantly lower than those of CD5(-) patients. In the condition of different GCB type, different therapy and low IPI (0 ∼ 2), the OS of CD5(+) DLBCL patients was significantly lower than that of CD5(-) patients, while in the condition of high IPI (3 ∼ 5), the OS of CD5(+) and CD5(-) DLBCL patient had no obvious difference.</p><p><b>CONCLUSIONS</b>CD5 expression is an adverse prognostic factor in DLBCL and it has more prognostic value in the condition of low IPI (0 ∼ 2).</p>


Subject(s)
Age Factors , Antibodies, Monoclonal, Murine-Derived , Therapeutic Uses , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , CD5 Antigens , Metabolism , Cyclophosphamide , Therapeutic Uses , Disease-Free Survival , Doxorubicin , Therapeutic Uses , Female , Humans , Interferon Regulatory Factors , Metabolism , Lymphoma, Large B-Cell, Diffuse , Classification , Drug Therapy , Metabolism , Pathology , Male , Middle Aged , Neprilysin , Metabolism , Prednisone , Therapeutic Uses , Proto-Oncogene Proteins c-bcl-6 , Metabolism , Retrospective Studies , Survival Rate , Vincristine , Therapeutic Uses
8.
Chinese Medical Journal ; (24): 2076-2079, 2012.
Article in English | WPRIM | ID: wpr-244410

ABSTRACT

Coexistence of chronic lymphocytic leukemia (CLL) and essential thrombocythemia (ET) in a patient is extremely rare, with only 10 cases reported thus far in literature. This paper describes a 94-year-old male having atypical B-CLL with CD5⁻ (CD5⁻) phenotype and ET. In this patient, we performed interphase fluorescence in situ hybridization (FISH) analysis which revealed 13q14.3 deletion in 31% of B-lymphocyte nuclei and RB1 deletion in 27% of B-lymphocyte nuclei, but not in neutrophils and T-lymphocytes. Furthermore, we identified JAK2 V617F mutation in the peripheral blood nucleated cells and neutrophils, but not in the B- and T-lymphocyte populations. Therefore, it was concluded that the occurrence of CD5− B-CLL and ET in this patient was pathogenically independent.


Subject(s)
Aged, 80 and over , CD5 Antigens , Metabolism , Humans , In Situ Hybridization , Janus Kinase 2 , Genetics , Leukemia, Lymphocytic, Chronic, B-Cell , Genetics , Metabolism , Male , Mutation , Thrombocythemia, Essential , Genetics , Metabolism
9.
Article in English | WPRIM | ID: wpr-43978

ABSTRACT

The coexistence of CCND1/IGH and MYC rearrangements in mantle cell lymphoma (MCL) is a rare finding associated with a very poor prognosis. In this study, a patient with blastoid variant (MCL) is reported. The disease was clinically aggressive and refractory to chemotherapy, and the patient only survived for 1 month following diagnosis. Conventional cytogenetic study, FISH, and multicolor FISH (mFISH) demonstrated the involvement of the BCL1/CCND1 locus in a complex translocation, t(3;11)(q25;p15)t(11;14)(q13;q32). In addition, subclonal abnormalities in the 8q24 region, manifested as a t(8;14)(q24;q32)/MYC rearrangement, were identified. To the best of our knowledge, this is the first MCL case in Korea bearing these complex genomic aberrations.


Subject(s)
Aged, 80 and over , CD5 Antigens/metabolism , Bone Marrow/immunology , Chromosomes, Human, Pair 11 , Chromosomes, Human, Pair 14 , Chromosomes, Human, Pair 3 , Gene Rearrangement , Humans , Immunophenotyping , In Situ Hybridization, Fluorescence , Lymphoma, Mantle-Cell/diagnosis , Male , Oncogene Proteins, Fusion/genetics , Proto-Oncogene Proteins c-myc/genetics , Translocation, Genetic
10.
Chinese Journal of Pathology ; (12): 235-239, 2011.
Article in Chinese | WPRIM | ID: wpr-261815

ABSTRACT

<p><b>OBJECTIVE</b>To study the immunophenotype and overall survival of diffuse large B-cell lymphoma (DLBCL) classified according to the 2008 World Health Organization classification of tumors of hematopoietic and lymphoid tissues.</p><p><b>METHODS</b>Five hundred cases of DLBCL were retrospectively analyzed with histologic review, immunohistochemistry, gene rearrangement study, in situ hybridization and fluorescence in situ hybridization. Follow-up data were collected. The overall survival rates of germinal center B-cell (GCB) and non-germinal center B-cell (non-GCB) subtypes, as well as those of DLBCL, not otherwise specified (NOS) and Epstein-Barr virus (EBV)-positive DLBCL of the elderly, were compared.</p><p><b>RESULTS</b>DLBCL-NOS was the commonest subtype which accounted for 77.2% (386/500) of the cases. EBV-positive DLBCL of the elderly, primary DLBCL of central nervous system, primary mediastinal (thymic) large B-cell lymphoma and T cell/histiocyte-rich large B-cell lymphoma accounted for 9.4% (47/500), 4.4% (22/500), 2.8% (14/500) and 2.6% (13/500), respectively. 68.5% (219/320) of DLBCL-NOS belonged to non-GCB subtype. The percentage of GCB subtype and CD5-positive subtype were 28.4% (91/320) and 3.1% (10/320), respectively. Comparison of the overall survival, GCB and non-GCB immunophenotypic groups have no significant difference (P = 0.93). And the same result in which of the EBV-positive DLBCL of the elderly and DLBCL-NOS group, before and after age matched (P = 0.13 and 0.28, respectively). A double-hit lymphoma was found by FISH detection, which presenting as gray zone lymphoma in morphology.</p><p><b>CONCLUSIONS</b>By using Hans algorithm, GCB and non-GCB subtypes show no significant difference in overall survival. EBV-positive DLBCL of the elderly and DLBCL-NOS also do not have significant difference in overall survival. Fluorescence in situ hybridization technique is helpful in identification of DLBCL with rare phenotypes.</p>


Subject(s)
Aged , Burkitt Lymphoma , Metabolism , Pathology , CD5 Antigens , Metabolism , Epstein-Barr Virus Infections , Pathology , Follow-Up Studies , Genes, Immunoglobulin Heavy Chain , Genes, bcl-2 , Germinal Center , Pathology , Herpesvirus 4, Human , Humans , Immunophenotyping , Interferon Regulatory Factors , Metabolism , Lymphoma, Large B-Cell, Diffuse , Classification , Genetics , Pathology , Middle Aged , Neprilysin , Metabolism , Oncogene Fusion , Prognosis , Proto-Oncogene Proteins c-bcl-6 , Metabolism , Retrospective Studies , Survival Rate
11.
Chinese Journal of Pathology ; (12): 815-819, 2011.
Article in Chinese | WPRIM | ID: wpr-358228

ABSTRACT

<p><b>OBJECTIVE</b>To study the possible loss of pan-T cell antigens CD2, CD3, CD5 and CD7 in Kikuchi's disease and to evaluate the role of T cell antigen loss in distinguishing benign from malignant T-cell lymphoid lesions.</p><p><b>METHODS</b>Formalin-fixed and paraffin-embedded tissues of 33 cases of Kikuchi's disease and 15 cases of reactive lymphoid hyperplasia were studied by EliVision immunohistochemical staining for CD2, CD3, CD5 and CD7.</p><p><b>RESULTS</b>Twenty-four of the 33 (72.7%) cases of Kikuchi's disease lost one or more of the pan-T cell antigens, including the loss of CD5 only (13 cases), CD7 only (1 case), CD2 only (1 case), CD2 and CD7 (2 cases), CD5 and CD7 (4 cases), CD2 and CD5 (2 cases), and CD2, CD7 and CD5 (1 case). Amongst these cases, the commonest antigen loss was CD5 (20 cases, 60.6%), followed by CD7 (8 cases, 24.2%) and CD2 (6 cases, 18.2%). Compared with the xanthomatous subtype of Kikuchi's disease, the loss of antigens was more commonly seen in the proliferative and necrotizing subtypes. Analysis of follow-up data showed that the loss of antigens in Kikuchi's disease was not significantly associated with the prognosis. In reactive lymphoid hyperplasia, the expression of CD2, CD3, CD5 and CD7 was seen in all cases with similar intensity, with no obvious pan-T cell antigen loss.</p><p><b>CONCLUSION</b>Loss of one or more pan-T cell antigens in Kikuchi's disease is demonstrated in present study, suggesting that the immunophenotypic pattern is not unique in T cell lymphoma.</p>


Subject(s)
Adolescent , Adult , Antigens, CD7 , Metabolism , CD2 Antigens , Metabolism , CD3 Complex , Metabolism , CD5 Antigens , Metabolism , Child , Child, Preschool , Female , Follow-Up Studies , Histiocytic Necrotizing Lymphadenitis , Allergy and Immunology , Pathology , Humans , Male , Middle Aged , Pseudolymphoma , Allergy and Immunology , Recurrence , T-Lymphocytes , Allergy and Immunology , Young Adult
12.
Chinese Medical Journal ; (24): 1517-1523, 2011.
Article in English | WPRIM | ID: wpr-353952

ABSTRACT

<p><b>BACKGROUND</b>The cause of late-onset hemorrhagic cystitis (LOHC) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) remains obscure. In clinical practice, some LOHC patients respond to immunosuppression. The aim of this study was to determine the immune pathogenesis of LOHC post allo-HSCT.</p><p><b>METHODS</b>With the diagnosis of LOHC, patients were given initial treatment consisting of fluid hydration, alkalization and forced diuresis, and empirical anti-viral therapy for 10 - 14 days or until a week after the virus became negative. The nonresponders were applied corticosteroid. Seven to ten days later, patients' response was evaluated. Along with treatment, CD19(+) B lymphocyte subsets were measured at various study points.</p><p><b>RESULTS</b>From October 2009 to March 2010, we found 28 cases of LOHC occurred in 25 patients who underwent allo-HSCT in our hospital. Except that three cases were not treated according to the protocol, the other 25 cases were divided into three groups: anti-virus responders (Group A, n = 6), corticosteroid responders (Group B1, n = 16), corticosteroid and anti-virus nonresponders (Group C, n = 3) according to their clinical response. Percentages of CD19(+)CD5(+) B lymphocytes were not significantly different among three groups at onset of LOCH. However, in Group B1 after the first anti-virus phase, percentages of CD19(+)CD5(+) lymphocytes significantly increased comparing with those at onset (P = 0.022), and then significantly decreased at PR (P = 0.003) and CR (P = 0.002) with corticosteroid treatment. But significant change was not observed in Groups A and C.</p><p><b>CONCLUSION</b>The immune etiology seems to be involved in the development of LOHC and the proportion of CD19(+)CD5(+) lymphocytes may serve as a cellular biomarker to predict the response to corticosteroid in LOHC.</p>


Subject(s)
Adolescent , Adrenal Cortex Hormones , Therapeutic Uses , Adult , Antigens, CD19 , Metabolism , B-Lymphocytes , Metabolism , CD5 Antigens , Metabolism , Child , Child, Preschool , Cystitis , Drug Therapy , Allergy and Immunology , Therapeutics , Female , Flow Cytometry , Hematopoietic Stem Cell Transplantation , Humans , Male , Middle Aged , Young Adult
13.
Chinese Journal of Pathology ; (12): 89-93, 2011.
Article in Chinese | WPRIM | ID: wpr-295103

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features, immunophenotypes and differential diagnosis of thyroid carcinoma showing thymus-like differentiation (CASTLE).</p><p><b>METHODS</b>The clinical and pathologic features of 8 cases of CASTLE were reviewed. Immunohistochemical study was performed using a panel of antibodies. In-situ hybridization for Epstein-Barr virus-encoded RNA (EBER) was also carried out.</p><p><b>RESULTS</b>There were altogether 4 males and 4 females. The age of the patients ranged from 25 to 57 years (mean = 48.8 years). All of them presented with painless mass at the anterior neck. Two patients also complained of hoarseness of voice. On CT scan, the tumor had a low density with contrast enhancement. Seven cases were located in the mid to lower pole and the remaining one in the upper pole of thyroid gland. Four cases were relatively circumscribed. The other 4 cases showed evidence of extrathyroidal invasion. Grossly, the tumor had a nodular or lobulated appearance and was gray-white in color, with a mean diameter of 4.3 cm. Microscopically, the tumor was infiltrative and consisted of islands, nests or lobules of epithelial cells separated by thick fibrous septa. The fibrous stroma showed various degree of lymphoplasmacytic infiltration, resulting in a prominent lymphoepithelioma-like pattern in 3 cases. Two cases showed squamoid differentiation, mimicking thymic Hassall corpuscles. Immunohistochemically, the tumor was consistently positive for cytokeratins, CD5, bcl-2, p63 and CD117. CEA was variably expressed. The staining for thyroglobin and TTF1 was negative. There was no labeling for EBER in all the cases. Two patients experienced local recurrence at 22 months and 12 years after surgery, respectively. They were treated with re-resection. All patients remained well on follow up. The duration of follow up ranged from 4 to 55 months.</p><p><b>CONCLUSIONS</b>CASTLE is a low-grade thyroid carcinoma with the morphologic features and immunophenotypes overlapping with those of thymic carcinoma. Awareness of this rare entity is important to both the pathologists and clinicians.</p>


Subject(s)
Adenoma , Metabolism , Pathology , General Surgery , Adult , CD5 Antigens , Metabolism , Carcinoma , Diagnostic Imaging , Metabolism , Pathology , General Surgery , Cell Differentiation , Disease-Free Survival , Female , Follow-Up Studies , Humans , Keratins , Metabolism , Male , Middle Aged , Neoplasm Recurrence, Local , Proto-Oncogene Proteins c-bcl-2 , Metabolism , Reoperation , Thymus Gland , Pathology , Thymus Neoplasms , Pathology , Thyroid Neoplasms , Diagnostic Imaging , Metabolism , Pathology , General Surgery , Tomography, X-Ray Computed
14.
Yonsei Medical Journal ; : 851-855, 2011.
Article in English | WPRIM | ID: wpr-182767

ABSTRACT

IL-10 production by CD19(+)CD5(+) B cells was investigated, by determining the expression levels of CD19, a classical B cell marker. Peripheral mononuclear cells were stained with fluorescence-conjugated anti-CD5, anti-CD19, anti-IL-10, and Annexin V. Interestingly, IL-10-producing B cells were found to be localised within the CD19(low)CD5(+) B cell subset. Apoptotic changes were also observed mainly in CD19(low) cells among B cells. Thus, CD5(+) B cells should be classified as CD19(high) and CD19(low) cells, and the immunological significance of CD19 for the IL-10 production by CD5(+) B cells requires further studies.


Subject(s)
Antigens, CD19/metabolism , CD5 Antigens/metabolism , Apoptosis/immunology , B-Lymphocyte Subsets/cytology , Cell Separation , Flow Cytometry , Humans , Interleukin-10/biosynthesis
15.
Article in English | WPRIM | ID: wpr-58116

ABSTRACT

Waldenstrom macroglobulinemia (WM) is a B-cell lymphoproliferative disorder associated with bone marrow involvement of lymphoplasmacytic lymphoma (LPL) and an IgM monoclonal gammopathy. Generally B-lymphocytes in LPL do not express CD5 that is important for differential diagnosis of B-cell lymphoproliferative disorders. In WM, various renal diseases and type I cryoglobulinemia are well described separately, but cryoglobulinemic glomerulonephropathy is very rarely reported. A 61-yr-old woman complained of generalized edema, cyanosis of the extremities in cold weather, visual disturbance, and pancytopenia. Bone marrow and renal biopsy showed CD5+ expressing B-cells and cryoglobulinemic glomerulonephropathy. With the diagnosis of WM, she received cyclophosphamide, doxorubicin, vincristine and prednisolone chemotherapy and got complete remission. Here, we report a rare case of WM associated with unusual expression of CD5+ B-lymphocytes and cryoglobulinemic glomerulonephropathy, and emphasize the importance of the clinical features in differentiating CD5+ B-cell lymphoproliferative disorders.


Subject(s)
CD5 Antigens/metabolism , Antineoplastic Agents/therapeutic use , B-Lymphocytes/immunology , Bone Marrow/pathology , Cryoglobulinemia/diagnosis , Cyclophosphamide/therapeutic use , Diagnosis, Differential , Doxorubicin/therapeutic use , Drug Therapy, Combination , Female , Glomerulonephritis/diagnosis , Humans , Kidney/pathology , Middle Aged , Paraproteinemias/diagnosis , Prednisolone/therapeutic use , Vincristine/therapeutic use , Waldenstrom Macroglobulinemia/diagnosis
16.
Chinese Journal of Pathology ; (12): 249-254, 2010.
Article in Chinese | WPRIM | ID: wpr-333294

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic characteristics of thymic epithelial tumors and to evaluate the diagnostic reproducibility and clinical relevance of the 2004 WHO histologic classification system.</p><p><b>METHODS</b>The morphology and immunophenotype of 52 cases of thymic epithelial tumor were reviewed. The tumors were classified according to the new WHO classification system and the clinical data were analyzed.</p><p><b>RESULTS</b>Of the 52 cases studied, 45 were thymomas and 7 were thymic carcinomas. Amongst the 45 cases of thymoma, 6 (13.4%) were type A, 15 (33.3%) were type AB, 4 (8.9%) were type B1, 9 (20.0%) were type B2, 9 (20.0%) were type B3 and 2 (4.4%) were metaplastic thymoma. Amongst the 7 cases of thymic carcinoma, 6 were squamous cell carcinomas and 1 was neuroendocrine carcinoma. The commonest presentations were cough and chest pain. Some cases were incidentally discovered by routine physical examination. Thirteen cases (25.0%) of thymoma were associated with myasthenia gravis. CT scan showed that 49 cases (94.2%) were located in the anterior mediastinum. All cases of type A, AB and B1 thymoma and most cases of B2 thymoma appeared as well-defined homogeneous mass, whereas a few cases of type B2 thymoma and most cases of type B3 thymoma and thymic carcinoma were poorly demarcated and heterogeneous. According to Masaoka staging system, 20 cases (41.7%) belonged to stage I, 15 cases (31.3%) stage II, 11 cases (22.9%) stage III and 2 cases (4.1%) stage IV. The histologic subtypes of thymic epithelial tumors significantly correlated with the clinical stages (chi(2) = 32.5, P < 0.01).</p><p><b>CONCLUSIONS</b>The 2004 revision of WHO histologic classification system for thymic epithelial tumors shows a high degree of reproducibility. Correlation with the radiologic, clinical and prognostic parameters is helpful in determining the management strategy for individual patients.</p>


Subject(s)
Adult , Aged , Antibodies, Monoclonal , Antigens, CD20 , Metabolism , CD5 Antigens , Metabolism , Carcinoma, Neuroendocrine , Classification , Diagnostic Imaging , Metabolism , Pathology , Carcinoma, Squamous Cell , Classification , Diagnostic Imaging , Metabolism , Pathology , Female , Follow-Up Studies , Humans , Keratins , Allergy and Immunology , Male , Middle Aged , Myasthenia Gravis , Neoplasm Recurrence, Local , Neoplasm Staging , Prognosis , Radiotherapy, Adjuvant , Retrospective Studies , Thymoma , Classification , Diagnostic Imaging , Metabolism , Pathology , Thymus Neoplasms , Classification , Diagnostic Imaging , Metabolism , Pathology , Tomography, X-Ray Computed
17.
Chinese Journal of Pathology ; (12): 804-809, 2010.
Article in Chinese | WPRIM | ID: wpr-295137

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic characteristics of gastric T-cell lymphoma.</p><p><b>METHODS</b>The clinicopathologic features of 7 cases of gastric T-cell lymphoma were retrospectively reviewed. Immunohistochemical study, T-cell receptor gene rearrangement analysis and evaluation of Epstein Barr virus (EBV) status were also performed.</p><p><b>RESULTS</b>The median age at onset of gastric T-cell lymphoma was 45 years. The male-to-female ratio was 6 to 1. The clinical information was available in 6 cases; and one of them had history of persistent diarrhea and 5 had hypoproteinemia. Histologically, 5 cases consisted of large lymphoma cells and the remaining 2 cases showed mainly medium-sized cells. Intraepithelial lymphoma cell infiltration was found in one case. The lymphoma cells of all cases were negative for CD20 and CD79a. CD3 and TIA-1 expression was noted in 6 of the 7 cases. CD5, βF-1 and CD30 were positive in 4 cases and CD4 was positive in 3 cases. In-situ hybridization for Epstein-Barr virus-encoded RNA was negative. Clonal T-cell receptor gene rearrangement was demonstrated in all cases.</p><p><b>CONCLUSION</b>Gastric T-cell lymphoma is a rare type of malignant lymphoma, with distinctive clinicopathologic characteristics.</p>


Subject(s)
Adult , Aged , CD3 Complex , Metabolism , CD5 Antigens , Metabolism , CD56 Antigen , Metabolism , CD8 Antigens , Metabolism , Female , Gene Rearrangement, T-Lymphocyte , Humans , Ki-1 Antigen , Metabolism , Lymphoma, T-Cell , Genetics , Metabolism , Pathology , General Surgery , Male , Middle Aged , Mitochondrial Proton-Translocating ATPases , Metabolism , RNA-Binding Proteins , Metabolism , Retrospective Studies , Stomach Neoplasms , Genetics , Metabolism , Pathology , General Surgery , Young Adult
18.
Article in Korean | WPRIM | ID: wpr-720076

ABSTRACT

Neoplastic lymphoid cells of chronic lymphocytic leukemia (CLL) typically co-express CD5 and CD23. CD5-negative CLL is a rare variant of CLL; only 1 case of it has been reported in Korea. We describe a case of CD5-negative CLL. A 48-year-old female complained of a palpable neck mass that had been present for over 1 year. The initial WBC count was 7,300/microliter, with 69% lymphocytes. A CT scan revealed multiple enlarged lymph nodes, both of each in the neck, axilla, and common iliac areas. The athologic results of the cervical lymph node was consistent with small lymphocytic lymphoma, of which tumor cells do not express CD5. In a bone marrow study, neoplastic lymphoid cells comprise 34.8% of all nucleated cells, which showed small size, round nuclei with clumped chromatin, and sparse cytoplasm. Immunophenotyping of small lymphoid cells displayed phenotypes that were CD45-, CD23-, CD20-, and CD19-positive, but CD5-negative. The patient was diagnosed with CD5-negative CLL, and has been followed up for 2.5 years after chemotherapy.


Subject(s)
CD5 Antigens , Axilla , Bone Marrow , Chromatin , Cytoplasm , Female , Humans , Immunophenotyping , Korea , Leukemia, Lymphocytic, Chronic, B-Cell , Lymph Nodes , Lymphocytes , Middle Aged , Neck , Phenotype
19.
Chinese Journal of Pathology ; (12): 749-753, 2009.
Article in Chinese | WPRIM | ID: wpr-249049

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features, immunophenotype and prognosis of primary cutaneous anaplastic large cell lymphoma (C-ALCL).</p><p><b>METHODS</b>Eight cases of C-ALCL were enrolled into the study. The clinicopathologic features, immunohistochemical findings and results of in-situ hybridization for EBER 1/2 were analyzed.</p><p><b>RESULTS</b>Three of the 8 patients were males and 5 were females. The median age was 49.5 years. C-ALCL often presented with solitary skin nodule, without systemic symptoms. Histologically, the lymphoma cells infiltrated the dermis and subcutis in a sheet-like pattern. They were of large size and showed conspicuous nuclear atypia. Immunohistochemical study showed that more than 75% of the lymphoma cells were positive for CD30. All cases expressed one to three T cell markers (CD3, CD5 or CD45RO) and cytotoxic granule-associated antigens (TIA-1, granzyme B or perforin). The staining for leukocyte common antigen was positive in all cases, while the expression of CD5, CD8, ALK-1 and epithelial membrane antigen was noted in 5, 1, 1 and 3 cases, respectively. The staining for CD15, CD20, CK and HMB45 was negative. In-situ hybridization for EBER 1/2 was also negative in all the cases studied. Follow-up information was available in 6 patients. Five of them were still alive and 1 died of unclear cause.</p><p><b>CONCLUSIONS</b>C-ALCL has distinctive clinicopathologic and immunophenotypic features. It is not Epstein-Barr virus-related and often carries a favorable prognosis.</p>


Subject(s)
Adult , Aged , CD5 Antigens , Metabolism , Child , Combined Modality Therapy , Female , Follow-Up Studies , Humans , Immunophenotyping , In Situ Hybridization , Ki-1 Antigen , Metabolism , Leukocyte Common Antigens , Metabolism , Lymphoma, Primary Cutaneous Anaplastic Large Cell , Allergy and Immunology , Metabolism , Pathology , Therapeutics , Male , Middle Aged , Prognosis , RNA, Viral , Metabolism , Skin Neoplasms , Allergy and Immunology , Metabolism , Pathology , Therapeutics , Young Adult
20.
Indian J Pathol Microbiol ; 2008 Jul-Sep; 51(3): 315-22
Article in English | IMSEAR | ID: sea-72871

ABSTRACT

We present clinical features, histopathology and results of treatment in cases of mantle cell lymphoma (MCL) at our hospital. We had 93 cases (2.1%) of MCL out of total 4301 cases of non-Hodgkin's lymphoma (NHL) in a 4-year period. It included 68 cases (1.7%) of MCL from 3987 cases of NHL diagnosed on histopathology. Remaining 25 cases (7.9%) diagnosed solely on peripheral blood examination were excluded. Thirty-six (85%) patients had advanced-stage disease. Sixty-three were nodal and five were extranodal (all gastrointestinal tract). Common patterns were diffuse (64%), nodular (25%) and mantle zone type (11%). Sixty-two cases had lymphocytic while six had blastic morphology (all nodal). Tumor cells expressed CD20 (100%), CD43 (94%), CD5 (89%) and cyclin D1 (85%). Bone marrow was involved in 25 (59%) cases. Thirty-two patients could be treated. Median recurrence-free survival was 22.23 months. Diffuse pattern of nodal involvement had a lower overall survival.


Subject(s)
Adult , Aged , Antigens, CD20/biosynthesis , Leukosialin/biosynthesis , CD5 Antigens/biosynthesis , Antineoplastic Agents/therapeutic use , Bone Marrow/pathology , Cyclin D1/biosynthesis , Female , Gastrointestinal Tract/pathology , Hospitals , Humans , India , Lymphoma, Mantle-Cell/drug therapy , Male , Middle Aged , Survival Analysis
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