Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 135
Filter
1.
Article in English | LILACS | ID: biblio-1353156

ABSTRACT

2021.174934ABSTRACTIntroduction: The mechanisms by which hepatitis C virus (HCV) infection induces autoimmune thyroiditis (AIT) have been studied, and it was suggested that inflammatory cytokines during HCV infection would change the thy-roperoxidase (TPO) signaling cascade and thyroglobulin (Tg) determining autoimmune thyroid disease.Objective: To show the signaling pathway, of TPO and Tg, and their potential targets mediated HCV in individuals with hepatitis C.Methods: The mapping of the signaling pathway was based on a review study approach and performed using the automatic annotation server of the Kyoto and Genome Encyclopedia (KEGG). PathVisio is free software for analysis and design of open source routes, and was used for the graphic representation of the signaling pathway.Results: The contigs were extracted from the KEGG database and their mapped transcription represents the signa-ling pathway of the main biomolecules that triggers the AIT. The action of HCV, or its treatment can trigger AIT that is characterized by the presence of autoantibodies against TPO and Tg. In AIT, autoreactive CD4 + T lymphocytes recruit B cells and CD8 + T cells in the thyroid. The progression of the disease leads to the death of thyroid cells and hypothyroidism. Conclusion: HCV or its treatment activates several signaling pathways with thyroid cells damage resulting in AIT and secondary hypothyroidism to cellular apoptosis. (AU)


RESUMOIntrodução: Os mecanismos pelos quais a infecção com o vírus da hepatite C (HCV) induz à tireoidite autoimune (TAI) têm sido alvo de estudos. Tem sido sugerido que citocinas inflamatórias, como a elevação das interleucinas na inflamação causadas pelo HCV, alterariam a cascata de sinalização da tireoperoxidase (TPO) e tireoglobulina (Tg) determinando um quadro de doença autoimune da tireóide.Objetivo: Demonstrar a via de sinalização da TPO e da Tg e seus potenciais alvos para a TAI mediados pelo HCV em indivíduos com hepatite C.Método: O mapeamento da via de sinalização foi realizado usando o servidor de anotação automática da Enciclopé-dia Quioto de Genes e Genomas (KEGG). O PathVisio, um software gratuito de análise e desenho de vias de código aberto, foi utilizado para a representação gráfica da via de sinalização.Resultado: As sequências foram retiradas do banco de dados KEGG e sua transcrição mapeada representa a via de . sinalização das principais biomoléculas que desencadeia a TAI. A ação do HCV, ou seu tratamento pode desen-cadear a TAI que é caracterizada pela presença de autoanticorpos contra a TPO e Tg. Na TAI os linfócitos T CD4+ auto-reativos recrutam células B e células T CD8+ na tireóide. A progressão da doença leva à morte de células da tireóide e hipotireoidismo.Conclusão: O HCV ou o seu tratamento ativa várias vias de sinalização com dano na célula tireoidiana, tendo como resultado TAI e hipotireoidismo secundário a apoptose celular. (AU)


Subject(s)
Humans , Autoimmune Diseases , Thyroid Diseases , Thyroiditis, Autoimmune , CD4 Antigens , CD8 Antigens , Hepacivirus , Disease Progression , Hypothyroidism
2.
Iatreia ; 33(4): 333-340, oct.-dic. 2020. tab
Article in Spanish | LILACS | ID: biblio-1143085

ABSTRACT

RESUMEN Objetivo: las personas infectadas con el virus de la inmunodeficiencia humana tipo 1 (VIH-1+) con un índice CD4:CD8 menor a 1, presentan un mayor riesgo de morbilidad y mor-talidad por eventos no asociados con el SIDA. El objetivo de este trabajo fue explorar‚ en la población seleccionada‚ variables sociodemográficas y clínicas de acuerdo con dicho índice, debido a que este es más informativo que LT CD4+ y LT CD8+ por sí solos. Materiales y métodos: estudio observacional en pacientes con VIH-1+ atendidos en la Corporación para Investigaciones Biológicas (CIB). En 227 pacientes se evaluaron diferencias en edad‚ recuento de LT CD4+‚ carga viral‚ número y tipo de esquemas. Se dividieron los pacientes en dos grupos: (A con índice CD4:CD8 ≥ 1 y, B < 1). Resultados: el estudio se compuso de la siguiente forma, 71 % hombres y 29 % mujeres. El 22,5 % pertenecía al grupo A y el 77,5 % al B. La media de la edad fue 42‚8 años en el grupo A y 45 en el B (p = 0‚176). El 100 % de los individuos en el grupo A recibían tratamiento y, 97‚7 % en el B. La media de LT CD4+ fue de 772‚4 para el grupo A y, 448‚1 en el B (p = 0‚00001). En el grupo A el 90‚2 % tenían carga viral indetectable‚ en contraste con el 68‚8 % del B (p = 0‚002). El 41‚2 % en el grupo A tuvieron un solo esquema‚ en relación con el 43,8 % del B (p = 0‚744). Conclusiones: la mayoría de los pacientes presentaron un índice CD4:CD8 < 1 a pesar de haber presentado LT CD4+ aceptables. Fue más frecuente encontrar un índice < 1 en los pacientes sin un adecuado control virológico. Se requieren más estudios para determinar las variables asociadas con su normalización.


SUMMARY Introduction: Human Immunodeficiency Virus type 1 (HIV-1+) patients with a CD4:CD8 ratio < 1 presents a higher risk of morbidity and mortality due to not-associated AIDS events. The aim was to explore, in the selected population, sociodemographic and clinical variables, based on that ratio, because it is more informative than LT CD4+ and LT CD8+ by themselves. Materials and Methods: Observational, in HIV-1 infected patients attended at Biological Research Corporation. In 227 patients, age differences, LT CD4+ count, viral load, number and type of treatments were evaluated. The patients were divided in group A with a CD4:CD8 ratio equal or above to 1, and B bellow 1. Results: The study includes 71% of male and 29% of female. 22,5% were from group A and 77,5% from B. The mean of age was 42,8 years old in A and 45,3 years old in B (p=0,176). 100% of individuals from group A receive treatment, meanwhile 97,7% in B. Mean of LT CD4+ count was 772,4 cell/μL in A and 448,1 cell/μL in B (p=0,00001). In A, 90,2% had undetectable viral load vs 68,8% in B (p=0,002). 41,2% in A had only one type of treatment, vs 43,8% in B (p=0,744). Conclusion: Most of the patients had a CD4:CD8 ratio bellow to 1, despite an acceptable count of LTCD4++. To find a ratio bellow 1 in patients without an adequate virological control was more frequent. More studies to determinate variables associated with its normalization are required.


Subject(s)
Humans , CD4 Antigens , Acquired Immunodeficiency Syndrome , HIV , CD8 Antigens , Mortality
3.
Oncol. (Guayaquil) ; 28(1): 62-72, 30 de Abril 2018.
Article in Spanish | LILACS | ID: biblio-999996

ABSTRACT

El sistema inmune cumple un rol fundamental en la defensa contra microorganismos y células anómalas. Históricamente, el concepto de vigilancia inmunológica se fundamenta en el control de múltiples funciones incluyendo la regulación de células cancerígenas a través de diversos mecanismos, en los cuales están involucrados: células, moléculas y tejidos del sistema inmune. El objetivo de analizar la respuesta inmune frente al cáncer, es entender los mecanismos de presentación del antígeno y los mecanismos desencadenados por el sistema adaptativo e innato que participan en la destrucción del tumor a expensas de un proceso inflamatorio agudo que podría llevar al control o destrucción del cáncer. La propuesta de esta revisión es resumir y esquematizar los aspectos cardinales de los diferentes procesos inmunológicos que participan en la fisiopatología de las enfermedades malignas, así como los mecanismos que emplea el sistema inmune para la defensa del cáncer.


The Inmune System plays an essential role in the defense of the organism against microorganisms and alters cells. Historically, the concept of immune surveillance its based in the control of multiple functions including the regulation of cancer cells through diverse mechanisms such as cells, molecules and tissue from the immune system. Therefore, it is important to understand the mechanisms of antigen presentation and other mechanisms of the innate and adaptive system which participate in the defense of the organism against the tumor. This process is enhancing by an inflammatory acute process that could lead to the control or de destruction of the tumor. The purpose of this review is to develop the cardinal aspects of the immunologic process that take part in the defense against malignant diseases, and also to explain its mechanisms.


Subject(s)
Humans , Immune System , Immunity , Antibody Formation , CD4 Antigens , CD8 Antigens , Antigens
4.
Rev. colomb. cancerol ; 21(1): 38-43, ene.-mar. 2017. tab, graf
Article in Spanish | LILACS | ID: biblio-900452

ABSTRACT

Resumen La proliferación linfoide indolente cutánea CD8 positiva es una variante recientemente descrita de linfoma T cutáneo que se caracteriza por un nódulo, pápula o placa eritematosa de crecimiento lento que puede afectar la región facial o extrafacial. En el estudio de patología se caracteriza por un infiltrado monomorfo de linfocitosTalo largo de la dermis con presencia de zona de Grenz y ausencia de epidermotropismo. El infiltrado es característicamente CD8+ así como CD3+, TIA-1+, CD4-, CD56- CD30-, PD-1-, Granzima B- y EBER negativo. El índice de proliferación Ki-67 es inferior al 10% y se observan reordenamientos clonales de los genes del receptor de antígeno de la célula T, TCR. El seguimiento clínico es favorable y no se ha observado compromiso sistémico. Se presentan tres casos con compromiso facial (dos casos en pabellón auricular y un caso con compromiso nasal), con presentación clínica y hallaz gos histopatológicos típicos (curiosamente un caso con cambio de célula clara), y además se realizaron estudios de clonalidad.


Abstract Primary cutaneous indolent CD8-positive lymphoid proliferation is a recent variant of cutaneous T lymphoma that is characterized by nodule, papule or plaque erythematous with slow growth that can affect the facial or extrafacial region. In the histopathology study it is characterized by an infiltration of monomorphic T lymphocytes throughout the dermis with presence of Grenz zone and absence of epidermotropism. The infiltrate is characteristically CD 8+ and CD3+ TIA-1+ CD4-, CD56- CD30, PD-1, Granzyme B- and negative EBER. Ki-67 Proliferación linfoide indolente cutánea CD8 positiva a propósito de tres casos proliferation index is less than 10% and clonal T-cell receptor gene rearrangements. Clinical follow-up is favorable and has not been observed systemic involvement. We present three cases with facial involvement (two cases in ear and one case with nasal commitment) with typical clinical presentation, histopathological findings (curiously a case with clear cell change) and clonality studies.


Subject(s)
Humans , Lymphoma, T-Cell, Cutaneous , CD8 Antigens , Cell Proliferation , Pathology , Genes, T-Cell Receptor , Ear Auricle
5.
Article in Chinese | WPRIM | ID: wpr-264007

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the correlation of the changes in CD8(+)CD28(-) T cell percentage with platelet (PLT) and D-dimer (D-D) levels in patients with multiple injuries (MI).</p><p><b>METHODS</b>Twenty-six patients with MI, 31 with a single injury (SI group) and 26 healthy individuals were examined for peripheral blood CD8(+)CD28(-) T cells and intracellular transformation growth factor-β1 (TGF-β1) and interleukin 10 (IL-10) contents using flow cytometry at 24, 48, and 72 h after the injuries. PLT and D-dimer levels were compared among the 3 groups.</p><p><b>RESULTS</b>CD8(+)CD28(-) T cells, TGF-β1 and IL-10 were significantly higher in MI group than in SI group and healthy control group (P<0.05) without significant differences between the latter 2 groups. The levels of PLT and D-D differed significantly among the 3 groups, the highest in MI group and the lowest in the control group. In MI group, CD8(+)CD28(-) T cells, TGF-β1 and IL-10 significantly increased at 48 h after the injury (P<0.05) but decreased significantly at 72 h (P<0.05) compared with the measurements at 24 h. The levels of PLT and D-D trended to decrease with time after the injuries and showed significant differences among the 3 groups at any of the 3 time points (P<0.05). CD8(+)CD28(-) T cells, TGF-β1 and IL-10 were all positively correlated with the levels of PLT and D-D in MI patients (r>0.70, P<0.05 for all comparisons).</p><p><b>CONCLUSION</b>In MI patients, CD8(+)CD28(-) T cell percentage and their cytokines tend to increase early after the injury but decrease significantly at 72 h in close relation with the changes of the coagulation function following the injuries.</p>


Subject(s)
CD28 Antigens , Metabolism , CD8 Antigens , Metabolism , Case-Control Studies , Fibrin Fibrinogen Degradation Products , Metabolism , Flow Cytometry , Humans , Interleukin-10 , Metabolism , Multiple Trauma , Allergy and Immunology , T-Lymphocyte Subsets , Cell Biology , Transforming Growth Factor beta1 , Metabolism
6.
Braz. oral res. (Online) ; 30(1): e34, 2016. graf
Article in English | LILACS | ID: biblio-951990

ABSTRACT

Abstract Interleukin 17A (IL-17A) is a proinflammatory cytokine responsible for the initiation and propagation of inflammation. One of its actions is the recruitment of neutrophils to the site of infection. The aim of this study was to investigate whether there is association between IL-17A expression and neutrophil infiltration in periapical abscesses and periapical granulomas, as well as to find which type of T lymphocyte effector (CD4+ or CD8+) expresses IL-17A in these lesions. Elastase, CD4, CD8, and IL-17A were analyzed by immunohistochemistry and immunofluorescence, in the biopsies of periapical lesions. Abscess lesions exhibited the highest labeling area for IL-17A (p = 0.011). During double immunofluorescence staining, there were significantly more CD4+/IL-17A+ cells compared to CD8+/IL-17A+ cells, both in the abscesses (p = 0.025) and granulomas (p = 0.011). In conclusion, IL-17A was intensively expressed in periapical abscesses rich in neutrophils. The high percentage of IL-17A in these cases suggests the participation of this cytokine particularly in the acute stages of the inflammatory process of the periapical lesions.


Subject(s)
Humans , Periapical Abscess/metabolism , Periapical Granuloma/metabolism , Periapical Granuloma/pathology , Interleukin-17/analysis , Periapical Abscess/pathology , Reference Values , Biopsy , Immunohistochemistry , Pancreatic Elastase/analysis , CD4-Positive T-Lymphocytes/pathology , CD4-Positive T-Lymphocytes/chemistry , CD4 Antigens/analysis , Fluorescent Antibody Technique , CD8 Antigens/analysis , CD8-Positive T-Lymphocytes/pathology , CD8-Positive T-Lymphocytes/chemistry , Neutrophil Infiltration
7.
Rev. bras. reumatol ; 55(3): 203-208, May-Jun/2015. tab, graf
Article in Portuguese | LILACS | ID: lil-752085

ABSTRACT

Objetivo: Analisar as frequências de expressão dos antígenos de complexo principal de histocompatibilidade classe I (MHC-I) e células CD4 e CD8 no músculo esquelético na polimiosite (PM) e dermatomiosite (DM). Métodos: Estudo retrospectivo de 34 casos de PM, oito casos de DM e 29 controles com miopatias não inflamatórias. Resultados: Os antígenos MHC-I expressaram-se no sarcolema e/ou sarcoplasma em 79,4% dos casos de PM, 62,5% dos casos de DM e 27,6% dos controles (a expressão de CD4 foi observada em 76,5%, 75% e 13,8%, respectivamente). Quando os antígenos de MHC-I foram coexpressados com CD4, houve elevada suspeita de PM/DM (principalmente PM). Em 14,3% dos casos de PM/DM, observou-se a expressão isolada dos antígenos MHC-I, sem células inflamatórias. Conclusão: A expressão dos antígenos MHC-I e a positividade do CD4 podem aumentar a suspeita diagnóstica de PM/DM. Não foi observado infiltrado celular em 14,3% dos casos. .


Objective: To analyze the frequencies of the expression of major histocompatibility complex class I (MHC-I) antigens, and CD4 and CD8 cells in skeletal muscle in polymyositis (PM) and dermatomyositis (DM). Methods: This was a retrospective study of 34 PM cases, 8 DM cases, and 29 control patients with non-inflammatory myopathies. Results: MHC-I antigens were expressed in the sarcolemma and/or sarcoplasm in 79.4% of PM cases, 62.5% of DM cases, and 27.6% of controls (CD4 expression was observed in 76.5%, 75%, and 13.8%, respectively). There was a high suspicion of PM/DM (mainly PM) in participants in whom MHC-I antigens and CD4 were co-expressed. In 14.3% of PM/DM cases, we observed MHC-I antigens expression alone, without inflammatory cells. Conclusion: MHC-I antigens expression and CD4 positivity might add to strong diagnostic suspicion of PM/DM. No cellular infiltration was observed in approximately 14.3% of such cases. .


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , CD4 Antigens/biosynthesis , CD8 Antigens/biosynthesis , Dermatomyositis/metabolism , Histocompatibility Antigens Class I/biosynthesis , Polymyositis/metabolism , CD4 Antigens/analysis , CD8 Antigens/analysis , Dermatomyositis/immunology , Histocompatibility Antigens Class I/analysis , Muscle, Skeletal/chemistry , Polymyositis/immunology , Retrospective Studies
8.
Chinese Journal of Hepatology ; (12): 955-957, 2015.
Article in Chinese | WPRIM | ID: wpr-303227

ABSTRACT

<p><b>OBJECTIVE</b>To determine the changes in levels of D-dimer, prothrombin time (PT), fibrinogen (Fib), CD4 and CD8 in relation to hepatopulmonary syndrome (HPS) by using a rat model system and to assess the association with pathologic changes in lung.</p><p><b>METHODS</b>Forty male Sprague-Dawley rats were divided into equal groups for modeling of cirrhosis and HPS. The two groups were assessed by blood gas analysis, standard biochemical tests to measure D-dimer, PT, Fib, CD4 and CD8, and pathological examination of lung tissues.</p><p><b>RESULTS</b>The HPS rats showed significantly lower PaO2 than the cirrhosis rats (58.20+/-3.19 mmHg vs. 85.00+/-2.53 mmHg, P = 0.000). The HPS rats showed significantly higher levels of D-dimer, Fib and CD8 than the cirrhosis rats (0.39+/-0.09 mg/ml vs. 0.25+/-0.05 mg/ml, P = 0.000; 1.77+/-0.10 g/L vs. and 1.49+/-0.09 g/L, P = 0.010; 32.32+/-4.45/mm3 vs. 20.13+/-6.09/mm3, P = 0.014). The HPS rats showed significantly lower levels of PT, CD4 and CD4/CD8 than the cirrhosis rats (14.86+/-1.04 s vs. 16.23+/-0.75 s, P = 0.036; 20.45+/-3.86/mm3 vs. 26.75+/-5.32/mm3, P = 0.000; 0.64+/-0.09 vs. 1.32+/-0.13, P = 0.000). The lung tissues of the HPS rats showed microthrombosis in pulmonary vessels, which were not observed in lung tissues of the cirrhosis rats.</p><p><b>CONCLUSION</b>HPS-related differential levels of D-dimer, PT, Fib, CD4, CD8 and CD4/CD8 may represent a biomarker profile suggestive of incidence of thromboembolism in lung.</p>


Subject(s)
Animals , CD4 Antigens , Metabolism , CD4-CD8 Ratio , CD8 Antigens , Metabolism , Disease Models, Animal , Fibrin Fibrinogen Degradation Products , Metabolism , Fibrinogen , Metabolism , Hepatopulmonary Syndrome , Blood , Liver Cirrhosis , Blood , Lung , Pathology , Male , Prothrombin Time , Rats , Rats, Sprague-Dawley
9.
IPMJ-Iraqi Postgraduate Medical Journal. 2014; 13 (3): 430-433
in English | IMEMR | ID: emr-149009

ABSTRACT

Immunophenotyping with monoclonal antibodies [MoAbs] directed against lymphoid-associated antigens, immunohistochemical staining on paraffin-embedded BM biopsy material, and molecular studies of Ig genes/T-cell receptor genes or lymphoma-associated gene translocations should be used in the global approach to the patient with malignant lymphoma. 1. To determine the subtypes of non-Hodgkin lymphomas [B- or T-cell] in the bone marrow using anti-CD3, CD8 monoclonal antibodies for T-cell and anti-CD19 and CD20 for B-cells. 2. Correlation of the subtypes of NHL [B- or T-cell] with the morphology and pattern of bone marrow infiltration. A retrospective study, done in Al-Kadhymia teaching hospital during the period from 1/10/2010 to1/2/2011.The study consisted 26 adult patients, who were diagnosed as Non-Hodgkin lymphomas by undergoing a BM biopsy. Immunohistochemical staining of the paraffin-embedded sections of BM trephine biopsies was performed in all cases and used standard techniques with monoclonal anti-CD8, CD20 and dual immunofluorscence-labelled CD3, and CD19 antibodies and also all stained with Hematoxylin and Eosin [H and E] for morphologic assessment. The 26 cases of NHL comprised of 14 male [54%] and 12 female patients [46%]. The median age was [57.32] year ranged from 27-85 years. There were 23 cases of B-cell cases [88.5%] and 3 cases of T-cell lineage [11.5%] of all the cases. Among all the B-cell lymphomas, 15 cases showed interstitial infiltration in the bone marrow, while among the T-cell lymphoma two cases showed diffuse infiltration. 1. In these 26 cases NHL patients with marrow involvement, B cell phenotype comprised 88% of cases. 2. B-cell NHLs had predominance of interstitial infiltration in bone marrow biopsies in comparison with the T-cell lymphoma, in which diffuse infiltration was predominant


Subject(s)
Humans , Male , Female , Bone Marrow/pathology , Immunophenotyping , CD3 Complex , CD8 Antigens , Antigens, CD19 , Antigens, CD20 , Antibodies, Monoclonal , T-Lymphocytes , B-Lymphocytes , Retrospective Studies
10.
Chinese Journal of Oncology ; (12): 910-913, 2013.
Article in Chinese | WPRIM | ID: wpr-329018

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the expression of co-stimulatory molecules PD-1/PD-L1 in peripheral blood mononuclear cells in lung cancer patients, and to explore its biological significance.</p><p><b>METHODS</b>One hundred and thirty-three lung cancer patients, 25 lung infection patients and 23 healthy donors were enrolled in this study. 100 µl of whole blood from these subjects were collected. Multi-color immunofluorescence staining and flow cytometry were used to detect PD-1/PD-L1 expression. The results were statistically analyzed.</p><p><b>RESULTS</b>The expression level of CD3⁺CD8⁺ T cells in the lung cancer patients was (38.83 ± 1.74)%, significantly lower than that in the control group [(43.25 ± 3.35)%, P < 0.05]. CD8⁺CD28⁺ T cell subset in the peripheral blood of lung cancer patients was (17.73 ± 1.21)% significantly lower than that of the healthy donors [(27.96 ± 2.72)%, P < 0.01]. The CD8⁺CD28⁻ T cell subset was (21.19 ± 1.92)% in the lung cancer patients, significantly higher than that of the healthy control group [(15.18 ± 2.93)%, P < 0.05]. The expression level of PD-1 on the surface of CD8⁺CD28⁺ T cells was (10.67 ± 1.12)% in the group of lung cancer patients, significantly higher than that of the control group [(5.32 ± 1.58)%, P < 0.01]. It was also found that the expression of PD-1 on CD8⁺CD28⁻ T cells was up-regulated in the group of lung cancer patients (7.46 ± 1.25)%, significantly higher than that of the healthy control group [(2.68+1.07)%, P < 0.01]. The expression level of PD-L1 on CD68⁺ cells in the lung cancer patients was (16.03 ± 2.06)%, significantly higher than that of the healthy control group [(9.32 ± 2.00)%, P < 0.05].</p><p><b>CONCLUSION</b>Up-regulation of PD-1/PD-L1 on peripheral blood cells in lung cancer patients negatively regulates the lymphocytes, inhibits the immune response for killing tumor cells, and promotes tumor development and immune escape.</p>


Subject(s)
Adenocarcinoma , Blood , Pathology , B7-H1 Antigen , Metabolism , CD28 Antigens , Metabolism , CD3 Complex , Metabolism , CD8 Antigens , Metabolism , Carcinoma, Large Cell , Blood , Pathology , Carcinoma, Squamous Cell , Blood , Pathology , Case-Control Studies , Female , Humans , Lung Neoplasms , Blood , Pathology , Male , Middle Aged , Programmed Cell Death 1 Receptor , Metabolism , Small Cell Lung Carcinoma , Blood , Pathology , T-Lymphocytes , Allergy and Immunology , Metabolism , Up-Regulation
11.
Article in English | WPRIM | ID: wpr-169635

ABSTRACT

Germanium biotite (GB) is an aluminosilicate mineral containing 36 ppm germanium. The present study was conducted to better understand the effects of GB on immune responses in a mouse model, and to demonstrate the clearance effects of this mineral against Porcine reproductive and respiratory syndrome virus (PRRSV) in experimentally infected pigs as an initial step towards the development of a feed supplement that would promote immune activity and help prevent diseases. In the mouse model, dietary supplementation with GB enhanced concanavalin A (ConA)-induced lymphocyte proliferation and increased the percentage of CD3+CD8+ T lymphocytes. In pigs experimentally infected with PRRSV, viral titers in lungs and lymphoid tissues from the GB-fed group were significantly decreased compared to those of the control group 12 days post-infection. Corresponding histopathological analyses demonstrated that GB-fed pigs displayed less severe pathological changes associated with PRRSV infection compared to the control group, indicating that GB promotes PRRSV clearance. These antiviral effects in pigs may be related to the ability of GB to increase CD3+CD8+ T lymphocyte production observed in the mice. Hence, this mineral may be an effective feed supplement for increasing immune activity and preventing disease.


Subject(s)
Aluminum Silicates/administration & dosage , Animal Feed/analysis , Animals , CD3 Complex/metabolism , CD8 Antigens/metabolism , Antiviral Agents/administration & dosage , Concanavalin A/metabolism , Dietary Supplements/analysis , Disease Models, Animal , Ferrous Compounds/administration & dosage , Germanium/administration & dosage , Lung/immunology , Lymphocyte Activation/drug effects , Lymphocytes/cytology , Lymphoid Tissue/immunology , Mice , Mitogens/metabolism , Porcine Reproductive and Respiratory Syndrome/drug therapy , Porcine respiratory and reproductive syndrome virus/drug effects , Swine
12.
Egyptian Journal of Histology [The]. 2013; 36 (4): 814-822
in English | IMEMR | ID: emr-160166

ABSTRACT

Tonsils contain four specialized lymphoid compartments that together are involved in immune functions. The capacity of tonsillar lymphocytes to counter infections may be altered during one's lifetime. The classification of lymphocytes by CD antigen expression is now widely used in clinical medicine and experimental immunology. The present work was designed to study the distribution of CD4 and CD8 antigen expression in T lymphocytes in human tonsils at different periods of life. Sixty-two tonsillar specimens were obtained from still birth infants and from children aged 1-9 years. Paraffin sections were prepared and stained with H and E and with immunohistochemical stains to demonstrate CD4 and CD8 T lymphocytes. The distribution of these cells in the different components of the tonsils was evaluated with an image analyzer. The obtained data were statistically analyzed using SPSS. There was a significant increase in the distribution of stained CD4 and CD8 T lymphocytes in the interfollicular areas, mantle zones of lymphoid follicles, and partially in the germinal centers of the examined tonsils with the advancement of age. Activated T lymphocytes differentiate into several subtypes, among which are CD4 and CD8 cells. These types of T lymphocytes express surface antigens, which can interact with different foreign pathogens


Subject(s)
Humans , Male , Female , Immunohistochemistry/statistics & numerical data , Diagnostic Techniques and Procedures/statistics & numerical data , T-Lymphocytes/immunology , CD8 Antigens/immunology , CD8 Antigens/blood , CD4 Antigens/blood , CD4 Antigens/immunology
13.
Medical Journal of Islamic World Academy of Sciences. 2013; 21 (2): 57-60
in English | IMEMR | ID: emr-143223

ABSTRACT

Determination of concentrations of cluster of differentiation [CD] markers in patients with atopic dermatitis and comparison with healthy individuals were carried out in this study. It was found that the mean concentrations of positive lymphocytes for AD patients reached 82.2%, 55.7%, 28.7%, and 21.9% for CD3, CD4, Cd8, and GD19, respectively, and those of healthy individuals reached 72.2, 40.3, 18.5, and 13.1 for CD3, CD4, Cd8, and CD19 respectively. We found that the mean values of CDs for AD patients high than those of healthy individuals [69.7%, 75.9%, 94.4% and 68.5%] [P<0.05]


re high than those of healthy individuals [65.7%, 75.9%, 94.4% and 68.5%] [P<0.05]


Subject(s)
Humans , Antigens, CD , Immunophenotyping , CD3 Complex , CD4 Antigens , CD8 Antigens , Antigens, CD19
14.
Chinese Journal of Pathology ; (12): 26-31, 2013.
Article in Chinese | WPRIM | ID: wpr-256264

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinicopathologic features, immunohistochemical findings, differential diagnosis and prognosis of type II enteropathy-associated T-cell lymphoma (EATL).</p><p><b>METHODS</b>Fourteen cases of type II EATL encountered in Department of Pathology, Nanjing General Hospital were retrospectively reviewed. The clinical data, histologic features, immunohistochemical findings and follow-up information were analyzed, with literature review.</p><p><b>RESULTS</b>There were altogether 12 males and 2 females. The median age of patient was 49 years. The sites of involvement included jejunum (10 cases) and ileum/colon (4 cases). The patients often presented with an abdominal mass, abdominal pain, diarrhea and constitutional symptoms such as fever, night sweating and cachexia. There was no clinical evidence of gluten-sensitive enteropathy. Histologically, the lymphoma cells showed full-thickness infiltration of the intestinal wall. They contained round hyperchromatic nuclei and pale cytoplasm. The stroma was minimally inflamed, with or without associated coagulative necrosis. A remarkable finding was the presence of villous atrophy, cryptal hyperplasia and intraepithelial lymphocytosis. Immunohistochemical study showed that the tumor cells expressed CD3, CD43 and CD8 (14/14). Some of them were also positive for CD56 (11/14) and CD30 (2/14). The staining for CD4, CD20, CD79a and myeloperoxidase was negative. A high proliferation index was demonstrated by Ki-67 immunostain. In-situ hybridization for EBER was negative. Follow-up data were available in 9 cases. The duration of follow-up ranged from 6 months to 36 months. Seven patients died within 14 months.</p><p><b>CONCLUSIONS</b>EATL is a rare type of lymphoma with intestinal involvement. Associated enteropathy is not demonstrated, in contrast to cases encountered in Nordic countries. A correct diagnosis requires evaluation of clinical manifestations, pathologic features and ancillary study results.</p>


Subject(s)
Adolescent , Adult , Aged , CD3 Complex , Metabolism , CD8 Antigens , Metabolism , Diagnosis, Differential , Enteropathy-Associated T-Cell Lymphoma , Genetics , Allergy and Immunology , Pathology , General Surgery , Female , Follow-Up Studies , Gene Rearrangement, T-Lymphocyte , Humans , Ileal Neoplasms , Genetics , Allergy and Immunology , Pathology , General Surgery , Jejunal Neoplasms , Genetics , Allergy and Immunology , Pathology , General Surgery , Leukosialin , Metabolism , Lymphoma, B-Cell, Marginal Zone , Metabolism , Pathology , Lymphoma, Extranodal NK-T-Cell , Metabolism , Pathology , Lymphoma, Large B-Cell, Diffuse , Metabolism , Pathology , Male , Middle Aged , Retrospective Studies , Young Adult
15.
Chinese Journal of Biotechnology ; (12): 1370-1377, 2012.
Article in Chinese | WPRIM | ID: wpr-342389

ABSTRACT

Wolynes argued that the track of a protein's folding was directed by the tendency of lowering its energy, and thus when a local minimum of its energy was reached, a relatively stable conformation was formed. However not all of the local minimums will lead the protein to a biologically useful conformation, for those otherwise are called energy traps. Wolynes energy landscape theory and natural selection have well explained the high efficiency of protein folding in vivo, instead of being stuck in energy traps. As to whether a protein can assume different conformations with the same bioactivity, there is no clear answer yet. In this paper, two conformational states of a pHLA-A*2402 are discovered after refolding, and by studying their interactions with TCR and CD8alphaalpha, two conformations of pHLA-A*2402 are confirmed of having escaped from natural selection.


Subject(s)
CD8 Antigens , Chemistry , Energy Metabolism , HLA-A24 Antigen , Chemistry , Humans , Protein Conformation , Protein Folding , Receptors, Antigen, T-Cell , Chemistry
16.
Chinese Medical Journal ; (24): 178-181, 2012.
Article in English | WPRIM | ID: wpr-333520

ABSTRACT

<p><b>BACKGROUND</b>Polyunsaturated omega-3 fatty acids may beneficially influence healing processes and patient outcomes. The aim of this research was to study the clinical efficacy of fish oil enriched total parenteral nutrition in elderly patients after colorectal cancer surgery.</p><p><b>METHODS</b>Fifty-seven elderly patients with colorectal cancer were enrolled in this prospective, randomized, double-blind, controlled clinical trial. All patients received isocaloric and isonitrogenous total parenteral nutrition by continuous infusion (20 - 24 hours per day) for seven days after surgery. The control group (n = 28) received 1.2 g/kg soybean oil per day, whereas the treatment group (n = 29) received 0.2 g/kg fish oil and 1.0 g/kg soybean oil per day. Blood samples were taken pre-operatively, and at days one and eight after the operation. The plasma levels of CD4, CD8, CD4/CD8, interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) were measured. Clinical outcomes were then analysed.</p><p><b>RESULTS</b>Patient characteristics were comparable between the two groups. At day eight post-surgery, IL-6, TNF-α and CD8 titres were lower in the treatment group when compared to the control group; these results reached statistical significance. In the treatment group, there were fewer infectious complications and incidences of systemic inflammatory response syndrome (SIRS), and shorter lengths of hospital stay were observed. The total cost of medical care was comparable for the two groups. No serious adverse events occurred in either group.</p><p><b>CONCLUSIONS</b>Fish oil 0.2 g/kg per day administrated to elderly patients after colorectal surgery was safe and may shorten the length of hospital stay and improve clinical outcomes.</p>


Subject(s)
Aged , CD4 Antigens , Blood , CD4-CD8 Ratio , CD8 Antigens , Blood , Colorectal Neoplasms , Blood , General Surgery , Colorectal Surgery , Female , Fish Oils , Therapeutic Uses , Humans , Interleukin-6 , Blood , Male , Middle Aged , Parenteral Nutrition, Total , Methods , Tumor Necrosis Factor-alpha , Blood
17.
Article in English | WPRIM | ID: wpr-80828

ABSTRACT

BACKGROUND: We developed a single-color multitarget flow cytometry (SM-FC) assay, a single-tube assay with graded mean fluorescence intensities (MFIs). We evaluated the repeatability of SM-FC, and its correlation with multicolor flow cytometry (MFC), to assess its application as a routine FC assay. METHODS: We selected CD19, CD3, CD4, and CD8 as antigen targets to analyze a lymphocyte subset. MFIs were graded by adjusting monoclonal antibody (mAb) volumes to detect several cell populations. Dimly labeled mAb was prepared by decreasing mAb volume and the optimum diluted volume was determined by serial dilution. SM-FC repeatability was analyzed 10 times in 2 normal controls. The correlation between SM-FC and MFC was evaluated in 20 normal and 23 patient samples. RESULTS: CV values (0.8-5.0% and 1.3-4.1% in samples 1 and 2, respectively) acquired by SM-FC with CD3-fluorescein alpha-isothyocyanate (FITC)dim+CD4-FITCbright and with CD19-FITCdim+CD3-FITCbright showed good repeatability, comparable to that acquired by MFC (1.6-3.7% and 1.0-4.8% in samples 1 and 2, respectively). Excellent correlation was observed between the 2 methods in the 20 normal samples (B cells, T cells, non-Thelper cells, and Thelper cells; r2=0.87, 0.97, 0.97, and 0.98, respectively; P or =0.98, 0.99, 0.99, and 0.99, respectively; P<0.05). CONCLUSIONS: The multicolor, single-tube SM-FC technique is a potential alternative tool for identifying a lymphocyte subset.


Subject(s)
Antibodies, Monoclonal/chemistry , Antigens, CD19/chemistry , CD3 Complex/chemistry , CD4 Antigens/chemistry , CD8 Antigens/chemistry , B-Lymphocyte Subsets/immunology , Color , Flow Cytometry/methods , Fluorescein-5-isothiocyanate/chemistry , Humans , T-Lymphocyte Subsets/immunology
18.
Chinese Journal of Pathology ; (12): 229-233, 2012.
Article in Chinese | WPRIM | ID: wpr-241946

ABSTRACT

<p><b>OBJECTIVE</b>To explore the hematopathologic features of T-cell large granular lymphocytic leukemia (T-LGLL).</p><p><b>METHODS</b>A retrospective analysis of the clinical presentation, bone marrow morphology, immunophenotyping and T-cell receptor gene rearrangement status were performed in 19 patients with T-LGLL.</p><p><b>RESULTS</b>Of 19 patients, the most frequent hematological abnormalities were anemia and neutropenia (16/19 and 17/19 patients, respectively). Large granular lymphocytes (LGLs) were observed in 17 of 19 peripheral blood smears and 15 of 19 bone marrow aspirate specimens. Lymphocytosis (> 0.2) was present in 17 of 19 patients in their bone marrow aspirate specimens. Bone marrow biopsy specimens revealed lymphocytosis in 16 cases, with a mild to moderate increase of lymphocytes observed in 12 cases (12/16). The pattern of lymphoid distribution was interstitial in bone marrow sections. Intravascular distribution was seen in 8 cases. Lymphoid nodules were present in 4 cases. Flow cytometery showed an immunophenotype of CD3(+) CD4(-) CD8(+) CD56(-) CD57(+) of the tumor cells in 13 cases. Of the other 6 cases, the immunophenotypes included CD8(-) (1 case), CD56(+) (2 cases) and CD57(-) (3 cases). Immunohistochemistry showed CD3+ (10/10), CD57+ (3/3), CD8+ (6/7), TIA-1+ (6/7), granzyme B+ (4/7), perforin + (1/7), CD4- (4/4) and CD56- (9/9). Clonal T-cell receptor γ gene rearrangement by PCR was detected in 12 cases (12/17).</p><p><b>CONCLUSIONS</b>Hematopathologic features of most T-LGLL are distinct. Morphologic, immunophenotypic and molecular analysis of both peripheral blood and bone marrow specimens are essential and complementary in the diagnosis and differential diagnosis of T-LGLL.</p>


Subject(s)
Adult , Aged , Anemia , Metabolism , Pathology , Bone Marrow , Pathology , CD3 Complex , Metabolism , CD57 Antigens , Metabolism , CD8 Antigens , Metabolism , Diagnosis, Differential , Female , Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor , Granzymes , Metabolism , Humans , Immunophenotyping , Leukemia, Large Granular Lymphocytic , Metabolism , Pathology , Lymphocytosis , Metabolism , Pathology , Male , Middle Aged , Neutropenia , Metabolism , Pathology , Poly(A)-Binding Proteins , Metabolism , Retrospective Studies , T-Cell Intracellular Antigen-1
19.
Chinese Journal of Cancer ; (12): 287-294, 2012.
Article in English | WPRIM | ID: wpr-294432

ABSTRACT

Establishing Epstein-Barr virus(EBV)-specific cytolytic T lymphocytes(EBV-CTLs) from peripheral blood mononuclear cells(PBMCs) for adoptive immunotherapy has been reported in EBV-associated malignancies including Hodgkin's lymphoma and nasopharyngeal carcinoma(NPC). In the current study, we performed ex vivo expansion of tumor-infiltrating lymphocytes(TILs) obtained from NPC biopsy specimens with a rapid expansion protocol using anti-CD3 monoclonal antibody(OKT3), recombinant human interleukin(IL)-2, and irradiated PBMCs from healthy donors to initiate the growth of TILs. Young TIL cultures comprised of more than 90% of CD3+ T cells, a variable percentage of CD3+CD8+ and CD3+CD4+ T cells, and less than 10% of CD3-CD16+ natural killer cells, a similar phenotype of EBV-CTL cultures from PBMCs. Interestingly, TIL cultures secreted high levels of the Th1 cytokines, interferon gamma (IFNγ) and tumor necrosis factor-alpha (TNF-α), and low levels of the Th2 cytokines, IL-4 and IL-10. Moreover, young TILs could recognize autologous EBV-transformed B lymphoblast cell lines, but not autologous EBV-negative blast cells or allogeneic EBV-negative tumor cells. Taken together, these data suggest that ex vivo expansion of TILs from NPC biopsy tissue is an appealing alternative method to establish T cell-based immunotherapy for NPC.


Subject(s)
Biopsy , CD3 Complex , CD4 Antigens , CD8 Antigens , Cells, Cultured , Herpesvirus 4, Human , Allergy and Immunology , Humans , Immunotherapy, Adoptive , Interferon-gamma , Metabolism , Interleukin-10 , Metabolism , Interleukin-2 , Pharmacology , Interleukin-4 , Metabolism , Lymphocytes, Tumor-Infiltrating , Allergy and Immunology , Virology , Monocytes , Pathology , Muromonab-CD3 , Pharmacology , Nasopharyngeal Neoplasms , Allergy and Immunology , Pathology , Therapeutics , Virology , Receptors, IgG , T-Lymphocytes, Cytotoxic , Allergy and Immunology , Virology , Tumor Necrosis Factor-alpha , Metabolism
20.
Journal of Experimental Hematology ; (6): 1212-1215, 2012.
Article in Chinese | WPRIM | ID: wpr-278404

ABSTRACT

This study was aimed to explore the effect of cordyceps sinensis enhancing lymphocyte proliferation and surface CD marker expression in simulated microgravity environment. The splenic lymphocytes were separated from mice and cultured in the rotary cell culture system simulated microgravity environment. The cells were treated with different concentration of cordyceps sinensis solution (0, 6.25, 12.5, 25 and 50 µg/ml) for 24, 48 and 72 h respectively, then the cells were harvested, and analyzed for cell proliferation and the expression of cell surface markers (CD4 and CD8). The results showed that under simulated microgravity environment, the lymphocyte proliferation was inhibited. When the concentration of cordyceps sinensis was 25 or 50 µg/ml, the lymphocyte proliferation, CD4 and CD8 expressions all increased, but 50 µg/ml cordyceps sinensis could inhibit the proliferation ability with the time prolonging. It is concluded that the suitable concentration of cordyceps sinensis displayed the ability to enhance the lymphocyte proliferation and CD marker expression in simulated microgravity environment. These results may be valuable for screening drugs which can be potentially against immunosuppression under simulated microgravity.


Subject(s)
Animals , CD4 Antigens , Metabolism , CD8 Antigens , Metabolism , Cell Proliferation , Cells, Cultured , Cordyceps , Immune Tolerance , Lymphocyte Activation , Lymphocytes , Metabolism , Mice , Mice, Inbred C57BL , Polysaccharides , Pharmacology , Spleen , Cell Biology , Weightlessness Simulation
SELECTION OF CITATIONS
SEARCH DETAIL