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1.
Braz. dent. j ; 32(4): 8-18, July-Aug. 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1345521

ABSTRACT

Abstract The aim of this study was to evaluate the physicochemical properties, cytocompatibility and antibiofilm activity of a new calcium silicate-based endodontic sealer, Sealer Plus BC (MK Life, Brazil), in comparison with TotalFill BC Sealer (FKG Dentaire SA, Switzerland) and AH Plus (Dentsply, Germany). Setting time and flow were evaluated based on ISO 6876 standard. The pH was evaluated after different periods, and radiopacity by radiographic analysis (mmAl). Solubility (% mass loss) and volumetric change (by micro-CT) were assessed after 30 days of immersion in distilled water. Cytocompatibility was assessed by methyltetrazolium (MTT) and neutral red (NR) assays, after exposure of Saos-2 cells to the sealer extract for 24 h. An additional analysis was performed by using MTT assay after 1, 3 and 7 days of exposure of Saos-2 to the sealers 1:8 dilution extracts. Antibiofilm activity against Enterococcus faecalis and/or Candida albicans was evaluated by crystal violet assay and modified direct contact test. The physicochemical properties were analyzed using ANOVA/Tukey tests; MTT and NR data were analyzed by ANOVA and Bonferroni tests; the antimicrobial tests were analyzed by Kruskal-Wallis and Dunn tests (α=0.05). Sealer Plus BC had proper setting time, radiopacity, flow and alkalization capacity. Sealer Plus BC was significantly more soluble than AH Plus (p<0.05) and presented volumetric change similar to AH Plus and TotalFill BC (p>0.05). Sealer Plus BC presented antibiofilm activity and no cytotoxic effect. In conclusion, although Sealer Plus BC had higher solubility, this sealer showed proper physicochemical properties, cytocompatibility, and antibiofilm activity.


Resumo O objetivo deste estudo foi avaliar as propriedades físico-químicas, a citocompatibilidade e a atividade antibiofilme de um novo cimento endodôntico à base de silicato de cálcio, Sealer Plus BC (MK Life, Brasil), em comparação com TotalFill BC Sealer (FKG Dentaire SA, Suíça) e AH Plus (Dentsply, Alemanha). O tempo de presa e o escoamento foram avaliados com base nas normas ISO 6876. O pH foi avaliado após diferentes períodos, e a radiopacidade por análise radiográfica (mmAl). A solubilidade (% de perda de massa) e alteração volumétrica (por micro-CT) foram avaliadas após 30 dias de imersão em água destilada. Citocompatibilidade foi avaliada pelos ensaios metiltetrazólio (MTT) e vermelho neutro (NR), após exposição das células Saos-2 ao extrato de cimento por 24 horas. Análise adicional foi realizada através do ensaio MTT após 1, 3 e 7 dias de exposição das células Saos-2 aos extratos dos cimentos na diluição de 1:8. Atividade antibiofilme contra Enterococcus faecalis e/ou Candida albicans foi avaliada pelos ensaios cristal violeta e contato direto modificado. As propriedades físico-químicas foram analisadas utilizando os testes ANOVA e Tukey; MTT e NR foram analisados pelos testes ANOVA e Bonferroni; os ensaios antimicrobianos foram analisados pelos testes Kruskal-Wallis e Dunn (α=0.05). Sealer Plus BC apresentou tempo de presa, radiopacidade e escoamento adequados, além de capacidade de alcalinização. Sealer Plus BC foi significantemente mais solúvel que AH Plus (p<0.05) e apresentou alteração volumétrica similar à de AH Plus e TotalFill BC (p>0.05). Sealer Plus BC apresentou atividade antibiofilme, sem efeito citotóxico. Como conclusão, embora Sealer Plus BC apresente maior solubilidade, este cimento apresentou propriedades físico-químicas adequadas, citocompatibilidade e atividade antibiofilme.


Subject(s)
Root Canal Filling Materials/pharmacology , Materials Testing , Silicates/pharmacology , Calcium Compounds/pharmacology , Biofilms , Epoxy Resins
2.
J. appl. oral sci ; 28: e20190023, 2020. graf
Article in English | LILACS | ID: biblio-1056577

ABSTRACT

Abstract When exposure of the pulp to external environment occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain pulp tissue vitality and function. These clinical situations require the use of materials that induce dentin repair and, subsequently, formation of a mineralized tissue. Objective: This work aims to assess the effect of tricalcium silicate cements and mineral trioxide aggregate cements, including repairing dentin formation and inflammatory reactions over time after pulp exposure in Wistar rats. Methodology: These two biomaterials were compared with positive control groups (open cavity with pulp tissue exposure) and negative control groups (no intervention). The evaluations were performed in three stages; three, seven and twenty-one days, and consisted of an imaging (nuclear medicine) and histological evaluation (H&E staining, immunohistochemistry and Alizarin Red S). Results: The therapeutic effect of these biomaterials was confirmed. Nuclear medicine evaluation demonstrated that the uptake of 99mTc-Hydroxymethylene diphosphonate (HMDP) showed no significant differences between the different experimental groups and the control, revealing the non-occurrence of differences in the phosphocalcium metabolism. The histological study demonstrated that in mineral trioxide aggregate therapies, the presence of moderate inflammatory infiltration was found after three days, decreasing during follow-ups. The formation of mineralized tissue was only verified at 21 days of follow-up. The tricalcium silicate therapies demonstrated the presence of a slight inflammatory infiltration on the third day, increasing throughout the follow-up. The formation of mineralized tissue was observed in the seventh follow-up day, increasing over time. Conclusions: The mineral trioxide aggregate (WhiteProRoot®MTA) and tricalcium silicate (Biodentine™) present slight and reversible inflammatory signs in the pulp tissue, with the formation of mineralized tissue. However, the exacerbated induction of mineralized tissue formation with the tricalcium silicate biomaterial may lead to the formation of pulp calcifications


Subject(s)
Animals , Male , Oxides/pharmacology , Biocompatible Materials/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Dental Pulp/drug effects , Dentin/drug effects , Dentinogenesis/drug effects , Phosphoproteins/analysis , Pulpitis/pathology , Pulpitis/drug therapy , Sialoglycoproteins/analysis , Time Factors , Immunohistochemistry , Random Allocation , Reproducibility of Results , Extracellular Matrix Proteins/analysis , Dental Pulp Exposure/pathology , Dental Pulp Exposure/drug therapy , Rats, Wistar , Dental Pulp/pathology , Dental Pulp Capping/methods , Drug Combinations , Molecular Imaging/methods , Pulp Capping and Pulpectomy Agents/pharmacology , Odontoblasts/drug effects
3.
J. appl. oral sci ; 28: e20190105, 2020. tab, graf
Article in English | LILACS | ID: biblio-1056578

ABSTRACT

Abstract Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. Objective: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. Methodology: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). Results: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. Conclusion: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair.


Subject(s)
Humans , Mice , Calcium Compounds/pharmacology , Calcium Compounds/chemistry , Aluminum Compounds/pharmacology , Aluminum Compounds/chemistry , Dental Cements/pharmacology , Dental Cements/chemistry , Dental Pulp/cytology , Dental Pulp/drug effects , Oxides/pharmacology , Oxides/chemistry , Time Factors , Zinc Oxide/pharmacology , Zinc Oxide/chemistry , Bismuth/pharmacology , Bismuth/chemistry , Materials Testing , Calcium Chloride/pharmacology , Calcium Chloride/chemistry , Gene Expression/drug effects , Cell Survival/drug effects , Cells, Cultured , Reproducibility of Results , Silicates/pharmacology , Silicates/chemistry , Drug Combinations , Alkaline Phosphatase/analysis , Alkaline Phosphatase/drug effects , Odontoblasts/drug effects
4.
J. appl. oral sci ; 28: e20190215, 2020. graf
Article in English | LILACS, BBO | ID: biblio-1056582

ABSTRACT

Abstract Objective: This study evaluated the angiogenesis-enhancing potential of a tricalcium silicate-based mineral trioxide aggregate (ProRoot MTA), Biodentine, and a novel bioceramic root canal sealer (Well-Root ST) in human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPLSCs), and human tooth germ stem cells (hTGSCs). Methodology: Dulbecco's modified Eagle's medium was conditioned for 24 h by exposure to ProRoot MTA, Biodentine, or Well-Root ST specimens (prepared according to the manufacturers' instructions). The cells were cultured in these conditioned media and their viability was assessed with 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium (MTS) on days 1, 3, 7, 10, and 14. Angiogenic growth factors [platelet-derived growth factor (PDGF), basic fibroblast growth factor (FGF-2), and vascular endothelial growth factor (VEGF)] were assayed by sandwich enzyme-linked immunosorbent assay (ELISA) on days 1, 7, and 14. Human umbilical vein endothelial cell (HUVEC) migration assays were used to evaluate the vascular effects of the tested materials at 6-8 h. Statistical analyses included Kruskal-Wallis, Mann-Whitney U, and Friedman and Wilcoxon signed rank tests. Results: None of tricalcium silicate-based materials were cytotoxic and all induced a similar release of angiogenic growth factors (PDGF, FGF-2, and VEGF) (p>0.05). The best cell viability was observed for hDPSCs (p<0.05) with all tricalcium silicate-based materials at day 14. Tube formation by HUVECs showed a significant increase with all tested materials (p<0.05). Conclusion: The tricalcium silicate-based materials showed potential for angiogenic stimulation of all stem cell types and significantly enhanced tube formation by HUVECs.


Subject(s)
Humans , Root Canal Filling Materials/pharmacology , Stem Cells/drug effects , Ceramics/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Angiogenesis Inducing Agents/pharmacology , Periodontal Ligament/cytology , Periodontal Ligament/drug effects , Tooth Germ/cytology , Tooth Germ/drug effects , Biocompatible Materials/pharmacology , Materials Testing , Platelet-Derived Growth Factor/analysis , Platelet-Derived Growth Factor/drug effects , Enzyme-Linked Immunosorbent Assay , Cell Survival/drug effects , Reproducibility of Results , Fibroblast Growth Factor 2/analysis , Fibroblast Growth Factor 2/drug effects , Statistics, Nonparametric , Neovascularization, Physiologic/drug effects , Dental Pulp/cytology , Dental Pulp/drug effects , Vascular Endothelial Growth Factor A/analysis , Vascular Endothelial Growth Factor A/drug effects , Human Umbilical Vein Endothelial Cells/drug effects , Flow Cytometry
5.
Int. j. morphol ; 37(3): 792-799, Sept. 2019. tab, graf
Article in English | LILACS | ID: biblio-1012355

ABSTRACT

The aim of the present study was to examine the short-term biocompatibility of Endosequence Root Repair Material (ERRM) paste and white Mineral Trioxide Aggregate MTA by implanting them into polyethylene tubes in the subcutaneous connective tissue of rats. twenty five male Wistar rats, 3-4 months old, weighing 300-350 g, were used. The tubes were implanted dorsally into the subcutaneous connective tissues of the rats. Five animals were sacrificed at five examination time points: 1, 3, 5, 7 and 15 days. The connective tissues containing the implants were excised. These sections were studied qualitatively and quantitatively using a light microscope. An average value for each group was obtained by averaging the sum of all inflammatory cells counted in 10 randomly selected, separate areas. For the ERRM group: There was a significant increase in the number of inflammatory cells on days 1-3 and on days 5-7 (P ≤ 0.003 and P ≤ 0.024). In the WHITE MTA group, the mean values of the sum of the inflammatory cells during the periods 1-3 days and 5-7 days were statistically significant (P ≤ 0.001 and P ≤ 0.044, respectively) and the XILOPERCHA group: Difference was observed significant in the value of the sum of inflammatory cells during the period of 3-5 days (P ≤ 0.05). According to the results it can be concluded that both, ERRM as MTA, caused an inflammatory reaction, which decreased over time; suggesting that both materials are biocompatible; showing however the presence of a higher organization of collagen fibers around the implants of ERRM.


El objetivo del presente estudio fue evaluar la biocompatibilidad a corto plazo de Material de Reparación de la Raíz Endodóntica (MRRE) y el agregado de trióxido mineral (AgTM), implantándolos dentro de tubos de polietileno en el tejido conectivo subcutáneo de ratas. Se usaron 25 ratas Wistar macho, de 3-4 meses de edad, con peso de 300 a 350 g. Los tubos fueron implantados en el tejido conectivo subcutáneo del dorso de las ratas. Cinco animales fueron sacrificados en cada uno de los siguientes períodos de tiempo: 1, 3, 5, 7, y 15 días. El tejido conectivo con los implantes fue escindido y seccionado. Los cortes se evaluaron cualitativa y cuantitativamente mediante microscopio óptico. Se obtuvo un valor para cada grupo resultado al promediar la suma de las células inflamatorias contadas en 10 áreas separadas seleccionadas aleatoriamente. Para el grupo de MRRE; hubo un incremento significativo en la cantidad de células inflamatorias entre los días 1-3 y 5-7 (p ≤ 0,003 y p ≤ 0,024). En el grupo de AgTM blanco, los valores promedio de la suma de células inflamatorias entre los períodos 1-3 días, y 5-7 días mostraron ser estadísticamente significativos (p≤ 0,001 y p ≤ 0,044 respectivamente) y en el grupo control de Xilopercha se observó diferencia significativa entre los valores de la suma de células inflamatorias entre los períodos de 3-5 días (P ≤ 0,05). De acuerdo a los resultados, puede concluirse que ambos materiales, AgTM y MRRE causaron una reacción inflamatoria que disminuyó a través del tiempo, sugiriendo que ambos materiales son biocompatibles; mostrando sin embargo una mayor organización de fibras colágenas alrededor de los implantes de MRRE.


Subject(s)
Animals , Male , Rats , Oxides/pharmacology , Calcium Phosphates/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Connective Tissue/drug effects , Root Canal Filling Materials/pharmacology , Materials Testing , Rats, Wistar , Drug Combinations
6.
Int. j. morphol ; 37(2): 685-689, June 2019. tab, graf
Article in Spanish | LILACS | ID: biblio-1002277

ABSTRACT

El Theracal TM LC es un cemento silicato de calcio (Ca) modificado con resina (SMCR) que ha demostrado ser un material ideal para el tratamiento dentino-pulpar por su alta tasa de formación de calcio. Los biomateriales por su contenido de Ca tienden a tener un aumento en su biodisponibilidad, estimulando la formación del puente dentario atreves de las células involucradas en la formación de tejidos mineralizados, promoviendo la diferenciación de fibroblastos en odontoblastos y aumentando la actividad de la enzima pirofostasa responsable en la mineralización de la dentina. El presente estudio con el objetivo de evaluar la respuesta inflamatoria a Theracal TM LC subcutáneamente en ratas Wistar. Fueron usados seis ratas cepa Wistar en las cuales se realizaron cuatro bolsillos quirúrgicos subcutáneos. Cada uno de estos bolsillos se determinó como cuadrante distinto, conteniendo los siguientes implantes: 1 Theracal TM LC en tubo polietileno, 2 tubo de polietileno, 3 Theracal TM LC directo y 4 como control. Las muestras histológicas se procesaron y se evaluaron distintos tipos celulares mediante conteo a microscopio de luz a 100X utilizando las tinciones H&E y AT pH 2.3. Los resultados mostraron que existen diferencias significativas en todos los tipos celulares observados durante los diferentes tiempos de exposición. Las diferencias en los tipos celulares observados podrían ser debido al tiempo de exposición al Theracal TM LC, al tubo polietileno y a ambos. El tejido evaluado del implante del tubo polietileno y al tubo polietileno con Theracal TM LC, presentan mayor respuesta inflamatoria, a diferencia en el tejido implantado con Theracal TM LC directamente.


TheraCalTM LC is a resin-modified calcium silicate (Ca) resin (SMCR) that has proven to be an ideal material for dentin-pulp treatment due to its high rate of calcium formation. Biomaterials due to their Ca content tend to have an increase in their bioavailability, stimulating the formation of the dental bridge through the cells involved in the formation of mineralized tissues, promoting the differentiation of fibroblasts in odontoblasts and increasing the activity of the pyrophosphate enzyme responsible in dentin mineralization. The present study aimed to evaluate the inflammatory response to TheracalTM LC subcutaneously in Wistar rats. Six Wistar strain rats were used in which four subcutaneous surgical pockets were made. Each of these pockets was determined as a different quadrant, containing the following implants: 1 TheracalTM LC in polyethylene tube, 2 polyethylene tubes, 3 TheracalTM LC direct and 4 as control. The histological samples were processed, and different cell types were evaluated by light microscopy at 100X using the H&E and AT pH 2.3 stains. The results showed that there are significant differences in all cell types observed during the different exposure times. The differences in the cell types observed could be due to the exposure time to TheracalTM LC, to the polyethylene tube and to both. The evaluated tissue of the polyethylene tube implant and the polyethylene tube with TheracalTM LC present a greater inflammatory response, unlike in the tissue implanted with TheracalTM LC directly.


Subject(s)
Animals , Rats , Calcium Compounds/pharmacology , Composite Resins/pharmacology , Subcutaneous Tissue/drug effects , Inflammation , Biocompatible Materials/pharmacology , Rats, Wistar , Silicates
7.
Braz. oral res. (Online) ; 33: e117, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-1132651

ABSTRACT

Abstract: The aim of this study was to evaluate the effect of mineral trioxide aggregate (MTA) and Brazilian propolis on the cell viability, mineralization, anti-inflammatory ability, and migration of human dental pulp cells (hDPCs). The cell viability was evaluated with CCK-8 kit after 1, 5, 7, and 9 days. The deposition of calcified matrix and the expression of osteogenesis-related genes were evaluated by Alizarin Red staining and real-time PCR after incubation in osteogenic medium for 21 days. The expression of inflammation-related genes in cells was determined after exposure to 1 μg/mL LPS for 3 h. Finally, the numbers of cells that migrated through the permeable membranes were compared during 15 h. Propolis and MTA significantly increased the viability of hDPCscompared to the control group on days 7 and 9. In the propolis group, significant enhancement of osteogenic potential and suppressed expression of IL-1β and IL-6 was observed after LPS exposure compared to the MTA and control groups. The number of migration cells in the propolis group was similar to that of the control group, while MTA significantly promoted cell migration. Propolis showed comparable cell viability to that of MTA and exhibited significantly higher anti-inflammatory and mineralization promotion effects on hDPCs.


Subject(s)
Humans , Oxides/pharmacology , Propolis/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Dental Pulp/cytology , Dental Pulp/drug effects , Anti-Inflammatory Agents/pharmacology , Brazil , Cell Movement/drug effects , Cell Survival/drug effects , Cells, Cultured , Reproducibility of Results , Anthraquinones , Interleukin-6/analysis , Tumor Necrosis Factor-alpha , Statistics, Nonparametric , Drug Combinations , Interleukin-1beta/analysis , Real-Time Polymerase Chain Reaction , Odontoblasts/drug effects
8.
Braz. oral res. (Online) ; 33: e060, 2019. tab, graf
Article in English | LILACS | ID: biblio-1011656

ABSTRACT

Abstract This study evaluated the effect of hypertension on tissue response and biomineralization capacity of white Mineral Trioxide Aggregate (MTA), High-plasticity MTA (MTA HP), and Biodentine® (BDT) in rats. Polyethylene tubes filled with MTA, MTA HP, BDT, and the control group (empty tubes) were placed into the dorsal subcutaneous tissue of 32 male rats (16 normotensive (NT) and 16 hypertensive rats - 8 per group). After 7 and 30 days, the polyethylene tubes surrounded by connective tissue were removed, fixed, and embedded in histological resin. The mean number of inflammatory cells was estimated in HE-stained sections, biomineralization was quantified as area (µm2) by Kossa (VK) staining, and examination by polarized light (LP) microscopy was performed. The differences amongst the groups were analyzed statistically by the Mann-Whitney or Student's t test, according to Shapiro-Wilk test of normality (p < 0.05). The inflammatory responses to all materials were greater in hypertensive rats than in NT rats (p < 0.05). Positive VK staining in MTA and BDT were more pronounced in NT rats at 7 and 30 days (p < 0.05). Birefringent structures in LP for MTA, MTA HP, and BDT were more pronounced in NT rats at 7 days (p<0.05). In rats, hypertension was able to increase inflammatory infiltrate and decrease biomineralization of the tested materials.


Subject(s)
Oxides/pharmacology , Biocompatible Materials/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Subcutaneous Tissue/drug effects , Subcutaneous Tissue/physiopathology , Biomineralization/physiology , Hypertension/physiopathology , Time Factors , Materials Testing , Reproducibility of Results , Rats, Wistar , Subcutaneous Tissue/pathology , Drug Combinations , Hypertension/complications , Inflammation/physiopathology , Inflammation/pathology , Microscopy, Polarization
9.
J. appl. oral sci ; 27: e20180550, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-1012512

ABSTRACT

Abstract Purpose To compare, both qualitatively and quantitatively, the inflammatory cells, vascular density and IL-6 immunolabeled cells present in the pulp after pulpotomy with white MTA versus 15.5% ferric sulfate (FS). Methodology Forty-eight mandibular first molars from 24 Wistar rats were divided into MTA or FS groups and subdivided according to the period after pulpotomy procedure (24, 48 and 72 hours). Four teeth (sound and untreated) were used as controls. Histological sections were obtained and assessed through the descriptive analysis of morphological aspects of pulp tissue and the quantification of inflammatory cells, vascular density and interleukin-6 (IL-6) expression. Data were statistically analyzed (p<0.05). Results The number of inflammatory cells was similar in both groups, being predominantly localized at the cervical radicular third. In the MTA group, increased inflammation was observed at 48 hours. Vascular density was similar in both groups and over time, being predominant in the medium radicular third. No correlation was found between the number of inflammatory cells and the vascular density. Pulp tissue was more organized in MTA-treated teeth. In both groups, a weak to moderate IL-6 expression was detected in odontoblasts and inflammatory cells. Comparing both groups, there was a greater IL-6 expression in the cervical radicular third of teeth treated with MTA at 24 hours and in the medium and apical thirds at 72 hours, while in the FS group a greater IL-6 expression was found in the apical third at 24 hours. Conclusion The MTA group presented better histological features and greater IL-6 expression than the FS group. However, no difference was observed between the groups regarding the inflammatory status and vascularization, suggesting the usefulness of FS as a low-cost alternative to MTA.


Subject(s)
Animals , Male , Oxides/pharmacology , Pulpotomy/adverse effects , Ferric Compounds/pharmacology , Interleukin-6/analysis , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Inflammation/immunology , Time Factors , Rats, Wistar , Statistics, Nonparametric , Dental Pulp/drug effects , Dental Pulp/pathology , Drug Combinations
10.
J. appl. oral sci ; 27: e20180429, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-990101

ABSTRACT

Abstract Objectives: To evaluate the radiopacity of Biodentine (BD) and BD associated with 15% calcium tungstate (BDCaWO4) or zirconium oxide (BDZrO2), by using conventional and digital radiography systems, and their physicochemical and biological properties. Materials and Methods: Radiopacity was evaluated by taking radiographs of cement specimens (n=8) using occlusal film, photostimulable phosphor plates or digital sensors. Solubility, setting time, pH, cytocompatibility and osteogenic potential were also evaluated. Data were analyzed using one-way ANOVA and Tukey post-test or two-way ANOVA and Bonferroni post-test (α=0.05). Results: BD radiopacity was lower than 3 mm Al, while BD ZrO2 and BD CaWO4 radiopacity was higher than 3 mm Al in all radiography systems. The cements showed low solubility, except for BDCaWO4. All cements showed alkaline pH and setting time lower than 34 minutes. MTT and NR assays revealed that cements had greater or similar cytocompatibility in comparison with control. The ALP activity in all groups was similar or greater than the control. All cements induced greater production of mineralized nodules than control. Conclusions: Addition of 15% ZrO2 or CaWO4 was sufficient to increase the radiopacity of BD to values higher than 3 mm Al. BD associated with radiopacifiers showed suitable properties of setting time, pH and solubility, except for BDCaWO4, which showed the highest solubility. All cements had cytocompatibility and potential to induce mineralization in Saos-2 cells. The results showed that adding 15% ZrO2 increases the radiopacity of BD, allowing its radiography detection without altering its physicochemical and biological properties.


Subject(s)
Humans , Zirconium/chemistry , Tungsten Compounds/chemistry , Silicates/chemistry , Calcium Compounds/chemistry , Radiography, Dental, Digital/methods , Osteoblasts/drug effects , Reference Values , Solubility , Time Factors , Zirconium/pharmacology , Materials Testing , Cell Survival/drug effects , Reproducibility of Results , Analysis of Variance , Anthraquinones , Tungsten Compounds/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Alkaline Phosphatase/analysis , Hydrogen-Ion Concentration
11.
J. appl. oral sci ; 27: e20180157, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-975884

ABSTRACT

Abstract Objectives The aim of this study was to evaluate the influence of surfactants 0.2% or 0.1% cetrimide (Cet) or 0.008% benzalkonium chloride (BAK) on 2.5% calcium hypochlorite (Ca(OCl)2), and compare to sodium hypochlorite (NaOCl), regarding the properties of pH, free chlorine content, surface tension, contact angle, pulp dissolution and antimicrobial activity. Material and Methods The pH and free chlorine content were evaluated by digital pHmeter and by titration, respectively. Surface tension was measured by the platinum ring technique with a Du Noüy tensiometer. The solution's contact angle in human dentin surfaces was checked by Drop Shape Analyzer software. Bovine pulps were used for pulp dissolution analysis and the dissolving capacity was expressed by percent weight loss. Antimicrobial activity over Enterococcus faecalis was evaluated by the agar diffusion method. Results Surfactants addition to Ca(OCl)2 and NaOCl did not alter the pH, free chlorine content and pulp dissolution properties. Ca(OCl)2 had the highest surface tension among all tested solutions. When surfactants were added to Ca(OCl)2 and NaOCl, there was a significant reduction of surface tension and contact angle values. The addition of 0.2% or 0.1% Cet enhanced antimicrobial activity of both Ca(OCl)2 and NaOCl. Conclusion Surfactant addition to 2.5% Ca(OCl)2 has shown acceptable outcomes for pH, free chlorine content, surface tension, contact angle, pulp dissolution and antimicrobial activity. Furthermore, the addition of 0.2% Cet showed better results for all tested properties.


Subject(s)
Humans , Animals , Cattle , Root Canal Irrigants/chemistry , Sodium Hypochlorite/chemistry , Surface-Active Agents/chemistry , Benzalkonium Compounds/chemistry , Calcium Compounds/chemistry , Cetrimonium/chemistry , Reference Values , Sodium Hypochlorite/pharmacology , Surface-Active Agents/pharmacology , Surface Properties , Benzalkonium Compounds/pharmacology , Materials Testing , Chlorine/analysis , Reproducibility of Results , Analysis of Variance , Enterococcus faecalis/drug effects , Calcium Compounds/pharmacology , Statistics, Nonparametric , Dental Pulp/drug effects , Dentin/drug effects , Cetrimonium/pharmacology , Hydrogen-Ion Concentration
12.
J. appl. oral sci ; 27: e20180150, 2019. graf
Article in English | LILACS, BBO | ID: biblio-975883

ABSTRACT

Abstract Objectives This investigation aimed to assess the differentiation inhibitory effects of ProRoot MTA® (PMTA) and Biodentine® (BIOD) on osteoclasts originated from murine bone marrow macrophages (BMMs) and compare these effects with those of alendronate (ALD). Materials and Methods Mouse BMMs were cultured to differentiate into osteoclasts with macrophage colony-stimulating factor and receptor activator of NF-κB (RANKL), treated with lipopolysaccharide. After application with PMTA, BIOD, or ALD, cell toxicities were examined using WST-1 assay kit, and RANKL-induced osteoclast differentiation and activities were determined by resorption pit formation assay and tartrate-resistant acid phosphate (TRAP) staining. The mRNA levels of osteoclast activity-related genes were detected with quantitative real time polymerase chain reaction. Expressions of molecular signaling pathways were assessed by western blot. All data were statistically analyzed with one-way ANOVA and Tukey's post-hoc test (p<0.05). Results Mouse BMMs applied with PMTA, BIOD, or ALD showed highly reduced levels of TRAP-positive osteoclasts. The BIOD treated specimens suppressed mRNA expressions of cathepsin K, TRAP, and c-Fos. Nonetheless, it showed a lower effect than PMTA or ALD applications. Compared with ALD, PMTA and BIOD decreased RANKL-mediated phosphorylation of ERK1/2 and IκBα. Conclusions PMTA and BIOD showed the inhibitory effect on osteoclast differentiation and activities similar to that of ALD through IκB phosphorylation and suppression of ERK signaling pathways.


Subject(s)
Animals , Mice , Osteoclasts/drug effects , Root Canal Filling Materials/pharmacology , Bone Marrow Cells/drug effects , Cell Differentiation/drug effects , Silicates/pharmacology , Calcium Compounds/pharmacology , Alendronate/pharmacology , Bone Density Conservation Agents/pharmacology , Osteoclasts/physiology , Osteogenesis/drug effects , Phosphorylation/drug effects , Root Resorption/prevention & control , Time Factors , Bone Marrow Cells/cytology , Cell Survival/drug effects , Cells, Cultured , Blotting, Western , Reproducibility of Results , MAP Kinase Signaling System/drug effects , I-kappa B Proteins/drug effects , RANK Ligand/analysis , RANK Ligand/drug effects , Real-Time Polymerase Chain Reaction , Tartrate-Resistant Acid Phosphatase
13.
J. appl. oral sci ; 27: e20180247, 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-975879

ABSTRACT

Abstract Objective This study aimed to investigate the effects of dodecacalcium hepta-aluminate (C12A7) content on some physicochemical properties and cytocompatibility of tricalcium silicate (C3S) cement using human dental pulp cells (hDPCs). Material and Methods High purity C3S cement was manufactured by a solid phase method. C12A7 was mixed with the cement in proportions of 0, 5, 8, and 10 wt% (C12A7-0, −5, −8, and −10, respectively). Physicochemical properties including initial setting time, compressive strength, and alkalinity were evaluated. Cytocompatibility was assessed with cell viability tests and cell number counts. Statistical analysis was performed by using one-way analysis of variance (ANOVA) and Tukey's test (p<0.05). Results The initial setting time of C3S-based cement was shorter in the presence of C12A7 (p<0.05). After 1 day, C12A7-5 showed significantly higher compressive strength than the other groups (p<0.05). After 7 days, the compressive strength of C12A7-5 was similar to that of C12A7-0, whereas other groups showed strength lower than C12A7-0. The pH values of all tested groups showed no significant differences after 1 day (p>0.05). The C12A7-5 group showed similar cell viability to the C12A7-0 group (p>0.05), while the other experimental groups showed lower values compared to C12A7-0 group (p<0.05). The number of cells grown on the C12A7-5 specimen was higher than that on C12A7-8 and −10 (p<0.05). Conclusions The addition of C12A7 to C3S cement at a proportion of 5% resulted in rapid initial setting time and higher compressive strength with no adverse effects on cytocompatibility.


Subject(s)
Humans , Silicates/chemistry , Calcium Compounds/chemistry , Aluminum Compounds/chemistry , Dental Cements/chemistry , Dental Pulp Cavity/cytology , Particle Size , Reference Values , Time Factors , X-Ray Diffraction , Biocompatible Materials/pharmacology , Biocompatible Materials/chemistry , Materials Testing , Microscopy, Electron, Scanning , Cell Survival/drug effects , Cells, Cultured , Reproducibility of Results , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Compressive Strength , Dental Cements/pharmacology , Dental Pulp Cavity/drug effects
14.
J. appl. oral sci ; 27: e20180693, 2019. graf
Article in English | LILACS, BBO | ID: biblio-1040226

ABSTRACT

ABSTRACT Objectives: To compare the sealing ability and biocompatibility of Biodentine with mineral trioxide aggregate (MTA) when used as root-end filling materials. Methodology: The Cell Counting Kit-8 (CCK-8) assay was used to compare the cytotoxicity of MTA and Biodentine. Twenty-one extracted teeth with a single canal were immersed in an acidic silver nitrate solution after root-end filling. Then, the volume and depth of silver nitrate that infiltrated the apical portion of the teeth were analyzed using micro-computed tomography (micro-CT). Seventy-two roots from 3 female beagle dogs were randomly distributed into 3 groups and apical surgery was performed. After six months, the volume of the bone defect surrounding these roots was analyzed using micro-CT. Results: Based on the results of the CCK-8 assay, MTA and Biodentine did not show statistically significant differences in cytotoxicity (P>0.05). The volume and the depth of the infiltrated nitrate solution were greater in the MTA group than in the Biodentine group (P<0.05). The volume of the bone defect was larger in the MTA group than in the Biodentine group. However, the difference was not significant (P>0.05). The volumes of the bone defects in the MTA and Biodentine groups were smaller than the group without any filling materials (P<0.05). Conclusions: MTA and Biodentine exhibited comparable cellular biocompatibility. Biodentine showed a superior sealing ability to MTA in root-end filling. Both Biodentine and MTA promoted periradicular bone healing in beagle dog periradicular surgery models.


Subject(s)
Humans , Animals , Male , Adolescent , Dogs , Oxides/pharmacology , Periapical Tissue/drug effects , Periodontal Ligament/drug effects , Root Canal Filling Materials/pharmacology , Root Canal Therapy/methods , Wound Healing/drug effects , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Osteogenesis/drug effects , Periapical Tissue/cytology , Periapical Tissue/diagnostic imaging , Periodontal Ligament/diagnostic imaging , Time Factors , Tooth Root/surgery , Tooth Root/drug effects , Tooth Root/diagnostic imaging , Bone Regeneration/drug effects , Materials Testing , Cell Count , Cells, Cultured , Reproducibility of Results , Treatment Outcome , Drug Combinations , X-Ray Microtomography
15.
Braz. dent. j ; 29(5): 459-464, Sept.-Oct. 2018. tab
Article in English | LILACS | ID: biblio-974175

ABSTRACT

Abstract The aim of this study was to compare the efficacy of grape seed extract (GSE), calcium hypochlorite [Ca(ClO)2], and sodium hypochlorite (NaOCl) irrigant solutions with rotary or reciprocating instrumentation for disinfection of root canals inoculated with Enterococcus faecalis. The mesiobuccal root canals of mandibular molars were prepared and inoculated with Enterococcus faecalis for 21 days. The roots were then randomly divided into the following eight experimental groups (n=11) according to the instrumentation technique and disinfection protocol: ProTaper Next or Reciproc R25 with sodium chloride (control group), 6% NaOCl, 6% Ca(ClO)2, or 50% GSE used for irrigation during instrumentation. The antimicrobial activity was determined on the basis of a reduction in colony-forming units (CFUs) counted on bacterial samples collected before and after root canal instrumentation and expressed as a percentage of reduction. Data were evaluated by two-way ANOVA followed by Tukey HSD post-hoc tests (p<0.05). No significant differences were observed in bacterial reduction between the ProTaper Next and Reciproc R25 systems (p>0.05), regardless of the irrigant solution used. Furthermore, all active solutions (6% NaOCl, 50% GSE, and 6% Ca(ClO)2) showed similar potential to reduce bacterial counts (p>0.05) and were significantly more effective than sodium chloride (control) (p<0.05). The results suggest that the GSE and Ca(ClO)2 have potential clinical application as irrigant solutions in endodontic therapy since they present bactericidal efficacy against Enterococcus faecalis.


Resumo O objetivo deste estudo foi comparar a eficácia do extrato de semente de uva (ESU), hipoclorito de cálcio [Ca(ClO)2] e hipoclorito de sódio (NaOCl) como soluções irrigadores quando utilizadas com instrumentos reciprocantes e rotatórios para desinfecção de canais radiculares infectados com Enterococcus faecalis. Raízes mesio-vestibulares de molares inferiores foram preparados e inoculados com E. faecalis por 21 dias. As raízes foram aleatoriamente divididas em 8 grupos (n=11) de acordo com a técnica de instrumentação e protocolo de irrigação: ProTaper Next ou Reciproc R25 associados com soro fisiológico (grupo controle), Ca(ClO)2 6%, NaOCl 6% ou ESU 50%. A atividade antimicrobiana foi determinada pela redução do número de Unidades Formadoras de Colonias (UFCs) coletadas antes e após a instrumentação e expressas em porcentagens de redução. Os dados foram analisados estatisticamente pelos testes ANOVA seguido pelo teste complementar de Tukey HSD (p<0,05). Não foi encontrado diferença estatisticamente significante na redução bacteriana entre os sistemas ProTaper Next e Reciproc R25 (p>0.05), independente da solução irrigadora usada. Além disso, todas as soluções ativas (NaOCl, ESU e Ca(ClO)2) mostraram similar potencial em reduzir a quantidade de bactérias (p>0.05) e foram significativamente mais efetivas que o soro fisiológico (p<0.05). Pode-se concluir que o ESU e o Ca(ClO)2 apresentam potencial para aplicação clínica como irrigantes endodônticos uma vez que apresentaram efetividade antimicrobiana contra o E. faecalis.


Subject(s)
Humans , Root Canal Irrigants/pharmacology , Disinfection/methods , Enterococcus faecalis/drug effects , Anti-Bacterial Agents/pharmacology , Sodium Hypochlorite/pharmacology , Stem Cells , In Vitro Techniques , Calcium Compounds/pharmacology , Composite Resins/chemistry , Dental Instruments , Dental Pulp Cavity/microbiology , Grape Seed Extract/pharmacology , Molar
16.
Braz. oral res. (Online) ; 32: e103, 2018. tab, graf
Article in English | LILACS | ID: biblio-974462

ABSTRACT

Abstract The aim of this study is to evaluate the expression of cytokines in response to mineral trioxide aggregate (MTA) plus selenium in germ-free mice with experimental furcal perforation. The first left maxillary molar was opened, and the furcal area was perforated and treated with post-MTA-Se (experimental group). The same surgical intervention was performed for the maxillary right first molar, which was treated with MTA (control group). Fifteen mice were sacrificed 7, 14, and 21 days after furcal perforation, and periapical tissue samples were collected. The mRNA expression levels of the cytokines TGF-β, TNF-α, IFN-γ, HPRT, IL-10, IL-4, RANK, RANKL, IL-1, and IL-17 were assessed by using real-time polymerase chain reaction. In the experimental group, at 21-days post-MTA-Se sealing, the mRNA levels of TNF-α and IL-10 were upregulated compared with those in the control group (p < 0.05). Futher assessment revealed basal mRNA expression levels of IL-1α, IFN-γ, RANK, RANKL, IL-17A, IL-4, and TGF-β, over long experimental times, in both the experimental and control groups (p > 0.05). In conclusion, MTA+Se sealing favoured increased expression of IL-10 and TNF-α at later time points (day 21).


Subject(s)
Animals , Male , Female , Oxides/pharmacology , Root Canal Filling Materials/pharmacology , Selenium/pharmacology , Cytokines/analysis , Silicates/pharmacology , Furcation Defects/drug therapy , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Dental Pulp Cavity/injuries , Root Canal Therapy/methods , Time Factors , Reproducibility of Results , Treatment Outcome , Furcation Defects/immunology , Dental Pulp Cavity/drug effects , Dental Pulp Cavity/immunology , Drug Combinations , Real-Time Polymerase Chain Reaction , Molar/drug effects , Molar/injuries
17.
Braz. oral res. (Online) ; 32(supl.1): e68, 2018. tab
Article in English | LILACS | ID: biblio-974474

ABSTRACT

Abstract: Endodontic medicine, which addresses the bidirectional relationship between endodontic infections and systemic diseases, has gained prominence in the field of endodontics. There is much evidence showing that while systemic disease may influence the pathogenesis of endodontic infection, endodontic infection can also cause systemic alterations. These alterations include more severe bone resorption and inflammation in the periapical area as well as enhanced systemic disease symptoms. Similarly, many reports have described the impact of systemic diseases on the tissue responses to dental materials. Conversely, the local use of dental materials may show systemic effects in the form of altered production of biomarkers. Thus, studies to better understand the mechanisms related to those connections are extremely important. In this context, the objective of this review was to analyze and discuss the current literature regarding the connections among these three factors—systemic diseases, endodontic infection, and endodontic dental materials—and determine how these connections may interfere in the systemic health status and the endodontic treatment outcomes, which are represented by periapical wound healing.


Subject(s)
Humans , Periapical Periodontitis/physiopathology , Root Canal Filling Materials/pharmacology , Cardiovascular Diseases/physiopathology , Subcutaneous Tissue/drug effects , Dental Pulp/drug effects , Diabetes Mellitus/physiopathology , Oxides/pharmacology , Risk Factors , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Dental Pulp Diseases/physiopathology , Drug Combinations , Metabolic Diseases/physiopathology
18.
J. appl. oral sci ; 26: e20160629, 2018. graf
Article in English | LILACS, BBO | ID: biblio-893696

ABSTRACT

Abstract Objective: The aim of the study was to evaluate the effects of the capping materials mineral trioxide aggregate (MTA), calcium hydroxide (CH) and BiodentineTM (BD) on stem cells from human exfoliated deciduous teeth (SHED) in vitro. Material and Methods: SHED were cultured for 1 - 7 days in medium conditioned by incubation with MTA, BD or CH (1 mg/mL), and tested for viability (MTT assay) and proliferation (SRB assay). Also, the migration of serum-starved SHED towards conditioned media was assayed in companion plates, with 8 μm-pore-sized membranes, for 24 h. Gene expression of dentin matrix protein-1 (DMP-1) was evaluated by reverse-transcription polymerase chain reaction. Regular culture medium with 10% FBS (without conditioning) and culture medium supplemented with 20% FBS were used as controls. Results: MTA, CH and BD conditioned media maintained cell viability and allowed continuous SHED proliferation, with CH conditioned medium causing the highest positive effect on proliferation at the end of the treatment period (compared with BD and MTA) (p<0.05). In contrast, we observed increased SHED migration towards BD and MTA conditioned media (compared with CH) (p<0.05). A greater amount of DMP-1 gene was expressed in MTA group compared with the other groups from day 7 up to day 21. Conclusion: Our results show that the three capping materials are biocompatible, maintain viability and stimulate proliferation, migration and differentiation in a key dental stem cell population.


Subject(s)
Humans , Oxides/pharmacology , Stem Cells/drug effects , Tooth, Deciduous/cytology , Calcium Hydroxide/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Pulp Capping and Pulpectomy Agents/pharmacology , Phosphoproteins/analysis , Stem Cells/physiology , Time Factors , Tooth, Deciduous/drug effects , Materials Testing , Cell Differentiation/drug effects , Cell Movement/drug effects , Cell Survival/drug effects , Cells, Cultured , Reproducibility of Results , Analysis of Variance , Extracellular Matrix Proteins/analysis , Reverse Transcriptase Polymerase Chain Reaction , Dental Pulp Capping/methods , Cell Proliferation/drug effects , Drug Combinations , Glyceraldehyde-3-Phosphate Dehydrogenases/drug effects
19.
Rio de janeiro; s.n; 2018. 76 p. ilus.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1016644

ABSTRACT

Este estudo teve como objetivo avaliar a biocompatibilidade, através de análise histopatológica e de imuno-histoquímica, de um novo cimento reparador à base de MTA com alta plasticidade: MTA HP (Angelus Londrina, PR). O MTA branco (Angelus Londrina, PR), e um material a base de óxido de zinco e eugenol (IRM, Dentsply, Petrópolis, RJ) foram utilizados como referências para comparação. Para isso, trinta ratos machos de linhagem Wistar tiveram inoculados no tecido subcutâneo um tubo de polietileno vazio (controle negativo) e mais três tubos, cada um preenchido com um dos materiais testados. Os animais foram eutanasiados após 7, 30 e 60 dias da implantação dos tubos e as amostras foram fixadas e incluídas em parafina. Os cortes histológicos foram corados com hematoxilina e eosina e tricômico de gomori para avaliação das reações inflamatórias e a presença de angiogênese foi realizada utilizando o marcador VEGF (do inglês vascular endothelial growth factor). Os cortes também foram corados com Picrosirius Red para quantificar as fibras colágenas do tipo I e tipo III, assim como a coloração de Weigert foi realizada para observar as fibras elásticas. Os dados não-paramétricos foram analisados usando o ensaio de Kruskal-Wallis seguido do teste de Dunn. Os níveis de significância adotados foram de 5% (P < 0,05). Os resultados mostraram diferença significativa da resposta inflamatória após 60 dias entre os grupos IRM e tubo vazio (P < 0,05). O MTA HP apresentou biocompatibilidade similar ao MTA branco e ao grupo controle negativo em todos os períodos experimentais. Além disso, após 7 dias o MTA HP estimulou a angiogênese de forma menos acentuada que o MTA branco, assim como apresentou inicialmente um remodelamento mais lento da matriz extracelular quando comparado ao MTA branco e o IRM. Foi observado uma diminuição da espessura da cápsula fibrosa, da quantidade de fibras elásticas e da imonumarcação com VEGF em todos os grupos experimentais e controle negativo ao longo do processo de cicatrização. Após 60 dias os grupos experimentais apresentaram matriz extracelular com tecido conjuntivo mais maduro, com predominância de fibras colágenas do tipo I. De acordo com os resultados obtidos no presente estudo, pode-se concluir que o novo cimento reparador com alta plasticidade, MTA HP, apresentou-se biocompatível em todos os períodos experimentais, com resultados similares aos grupos controle negativo e experimentais com MTA branco e IRM.


This study evaluated the biocompatibility, through histopathological analysis and immunohistochemistry of a new repair cement based on MTA with high plasticity: MTA HP (Angelus Londrina, PR). White MTA (Angelus Londrina, PR), and a material based on zinc oxide and eugenol (IRM, Dentsply, Petrópolis, RJ) were used as references for comparison. Thirty male Wistar rats had inoculated into the subcutaneous tissue an empty polyethylene tube (negative control) and three more tubes, each filled with one of the tested materials. The animals were euthanized after 7, 30 and 60 days of tube implantation and the specimens were fixed and embedded in paraffin. The sections were stained with hematoxylin and eosin and gomori trichrome to assess inflammatory reactions, and the presence of angiogenesis was performed using the VEGF (vascular endothelial growth factor) marker. The sections were also stained with Picrosirius Red to quantify as type I and type III collagen fibers, as well as a Weigert staining was performed to observe elastic fibers. Non-parametric data were analyzed using the Kruskal-Wallis assay followed Dunn's test. The significance levels adopted were 5% (P < 0.05). The results demonstrated a significant difference in inflammatory response after 60 days between IRM and empty tube groups (P < 0.05). MTA HP showed similar biocompatibility to the White MTA and the negative control group in all experimental periods. Furthermore, after 7 days MTA HP stimulated less pronounced angiogenesis than White MTA, as it initially exhibited slower extracellular matrix remodeling when compared to White MTA and IRM. A decrease in the thickness of the fibrous capsule, the amount of elastic fibers and the immunostaining with VEGF in all experimental groups and control throughout the healing process was observed. After 60 days, the experimental groups presented extracellular matrix with more mature connective tissue, with predominance of type I collagen fibers. According to the results obtained in the present study, it can be concluded that the new repair cement with high plasticity, MTA HP, was biocompatible in all the experimental periods, presenting similar results to the control and experimental groups with White MTA and IRM.


Subject(s)
Animals , Rats , Oxides/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Subcutaneous Tissue/drug effects , Dental Cements , Materials Testing , Immunohistochemistry , Rats, Wistar , Statistics, Nonparametric , Drug Combinations
20.
J. appl. oral sci ; 25(6): 620-630, Nov.-Dec. 2017. tab, graf
Article in English | LILACS, BBO | ID: biblio-893669

ABSTRACT

Abstract Objective: Several studies reported the local tissue reaction caused by mineral aggregate-based cements. However, few studies have investigated the systemic effects promoted by these cements on liver and kidney when directly applied to connective tissue. The purpose of this in vivo study was to investigate the systemic effect of mineral aggregate-based cements on the livers and kidneys of rats. Material and Methods: Samples of Mineral Trioxide Aggregate (MTA) and a calcium aluminate-based cement (EndoBinder) containing different radiopacifiers were implanted into the dorsum of 40 rats. After 7 and 30 d, samples of subcutaneous, liver and kidney tissues were submitted to histopathological analysis. A score (0-3) was used to grade the inflammatory reaction. Blood samples were collected to evaluate changes in hepatic and renal functions of animals. Results: The moderate inflammatory reaction (2) observed for 7 d in the subcutaneous tissue decreased with time for all cements. The thickness of inflammatory capsules also presented a significant decrease with time (P<.05). Systemically, all cements caused adverse inflammatory reactions in the liver and kidney, being more evident for MTA, persisting until the end of the analysis. Liver functions increased significantly for MTA during 30 d (P<.05). Conclusion: The different cements induced to a locally limited inflammatory reaction. However, from the systemic point of view, the cements promoted significant inflammatory reactions in the liver and kidney. For MTA, the reactions were more accentuated.


Subject(s)
Animals , Male , Rats , Oxides/pharmacology , Root Canal Filling Materials/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Dental Cements/pharmacology , Kidney/drug effects , Liver/drug effects , Time Factors , Biocompatible Materials , Materials Testing , Drug Combinations , Kidney/pathology , Liver/pathology
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