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Braz. dent. j ; 31(6): 598-604, Nov.-Dec. 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1132351


Abstract The aims of this study were to evaluate the physical and chemical properties, cytotoxicity and dentinal tubule penetration of a new calcium silicate-based root canal dressing. For pH and calcium ion release evaluation (1, 24, 72 and 168 h) were used a pH meter and colorimetric spectrophotometer, respectively. Radiopacity evaluation followed the ISO 6876:2012. Cytotoxicity was evaluated by the percentage of cell viability using MTT assay. Illustrative images of dentinal tubule penetration were obtained using confocal laser scanning microscopy (CLSM). Data from pH and calcium ion release were statistically analyzed by two-way analysis of variance and Tukey test. Radiopacity was analyzed using the Student t-test. The statistical tests for cytotoxicity results were the one-way analysis of variance and Tukey test. Both materials showed alkaline pH in all experimental times. The pH values for calcium hydroxide paste were higher than bioceramic paste at 1, 24, and 72 h (p<0.05). The calcium ion release of bioceramic was lower than the calcium hydroxide paste only at 24 h (p<0.05). The bioceramic was more radiopaque than the calcium hydroxide paste (p<0.05). Bioceramic paste presented a dose and time-dependent cytotoxic effect after MTT assay. CLSM images showed absence of tubule penetration for both pastes. The new calcium silicate-based canal dressing presented alkaline pH, high calcium release, and acceptable radiopacity. Bio C Temp showed a dose and time-dependent cytotoxic and absence of dentinal tubule penetration.

Resumo Os objetivos deste estudo foram avaliar as propriedades físicas e químicas, citototoxidade e penetração tubular de uma nova medicação à base de silicato de cálcio. Para o teste de pH, e liberação de íons cálcio (1, 24, 72 e 168 h) foi usado medidor de pH e espectofotômetro colorimétrico, respectivamente. Avaliação da radiopacidade, seguiu a ISO 6876:2012). A citotoxicidade foi avaliada pela porcentagem de células viáveis usando o ensaio MTT. Imagens ilustrativas de penetração tubular foram obtidas usando microscopia confocal de varredura a laser (CLSM). Os dados de pH e liberação de cálcio foram analisados através do teste de Análise de Variância de duas vias e teste de Tukey. A radiopacidade foi avaliada usando o teste T de Student. Para a citotoxicidade foi empregada a Análise de Variância de uma via e teste de Tukey. Ambos os materiais apresentaram pH alcalino em todos os tempos experimentais. Os valores de pH da pasta de hidróxido de cálcio foram superiores à pasta biocerâmica em 1, 24 e 72 h (p<0,05). A liberação de cálcio da pasta biocerâmica foi inferior à pasta de hidróxido de cálcio apenas em 24 h (p<0,05). Bio-C Temp foi mais radiopaco que o Ultracal XS (p<0,05). A pasta biocerâmica apresentou efeito citotóxico dependente da dose e do tempo de exposição. Imagens de CLSM mostraram ausência de penetração intratubular para ambas as pastas. A nova medicação à base de silicato de cálcio apresentou pH alcalino, alta liberação de cálcio e boa radiopacidade. Bio C Temp apresentou um efeito citotóxico dependente da dose e do tempo de exposição e ausência de penetração tubular.

Humans , Root Canal Filling Materials , Bandages , Calcium Hydroxide/toxicity , Silicates , Calcium Compounds/toxicity , Dental Pulp Cavity
Braz. oral res. (Online) ; 33: e042, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001597


Abstract: This study evaluated the cytotoxicity and biocompatibility of a new bioceramic endodontic sealer (i.e., Sealer Plus BC) in comparison with those of MTA Fillapex and AH Plus. L929 fibroblasts were cultured and Alamar Blue was used to evaluate cell viability of diluted extracts (1:50, 1:100, and 1:200) from each sealer at 24 h. Polyethylene tubes that were filled with material or empty (as a control) were implanted in the subcutaneous tissue of rats. The rats were killed after 7 and 30 d (n = 8), and the tubes were removed for histological analysis. Parametric data was analyzed using a one-way ANOVA test, and nonparametric data was analyzed via the Kruskal-Wallis test followed by the Dunn test (p < 0.05). A reduction in cell viability was observed in the extracts that were more diluted for Sealer Plus BC when compared to that of Control and AH Plus (p < 0.05). However, the 1:50 dilution of the Sealer Plus BC was similar to that of the Control (p > 0.05). Conversely, more diluted extracts of MTA Fillapex (1:200) and AH Plus (1:100 and 1:200) were similar to the Control (p > 0.05). Histological analysis performed at 7 d did not indicate any significant difference between tissue response for all materials, and the fibrous capsule was thick (p > 0.05). At 30 d, Sealer Plus BC was similar to the Control (p > 0.05) and MTA Fillapex and AH Plus exhibited greater inflammation than the Control (p < 0.05). The fibrous capsule was thin for the Control and for most specimens of Sealer Plus BC and AH Plus. Thus, Sealer Plus BC is biocompatible when compared to MTA Fillapex and AH Plus, and it is less cytotoxic when less-diluted extracts are used.

Animals , Male , Root Canal Filling Materials/chemistry , Bone Cements/chemistry , Calcium Hydroxide/chemistry , Ceramics/chemistry , Oxides/chemistry , Root Canal Filling Materials/toxicity , Biocompatible Materials , Bone Cements/toxicity , Bone Cements/pharmacology , In Vitro Techniques , Materials Testing , Calcium Hydroxide/toxicity , Calcium Hydroxide/pharmacology , Cell Survival/drug effects , Cells, Cultured/drug effects , Rats, Wistar , Silicates/chemistry , Calcium Compounds/blood , Aluminum Compounds/chemistry , Subcutaneous Tissue/pathology , Drug Combinations , Epoxy Resins/chemistry , Fibroblasts/drug effects , Inflammation
J. appl. oral sci ; 27: e20180291, 2019. graf
Article in English | LILACS | ID: biblio-984570


Abstract Objective The aim of this study was to investigate the cytotoxic effects of modified triple antibiotic paste and an experimental composition using calcium hydroxide on lipoteichoic acid (LTA)-primed apical papilla cells (APC). Material and Methods Human APC were tested for in vitro cytotoxicity of modified Triple Antibiotic Paste (mTAP - Ciprofloxacin, Metronidazole and Cefaclor at 1:1:1) and of a paste of Ciprofloxacin, Metronidazole and Calcium hydroxide (CMC - 1:1:2) and modified CMC (mCMC - 2:2:1) by using MTT assay. The substances were reconstituted in DMEM at 1,000 µg/mL and » serially diluted before being kept in contact with cells for 1, 3, 5 and 7 days. Further, cells were primed with 1 µg/mL of Enterococcus faecalis LTA for 7 days prior to the viability test with 1,000 µg/mL of each substance. Statistical analysis was performed using one-way analysis of variance (ANOVA) and two-way ANOVA respectively followed by Tukey's post-test. Significance levels were set at p<0.05. Results In the first assay, the higher cytotoxic rates were reached by mTAP for all experimental periods. CMC was found toxic for APC at 5 and 7 days, whereas mCMC did not affect the cell viability. Only CMC and mCMC were able to induce some cellular proliferation. In the second assay, when considering the condition with medium only, LTA-primed cells significantly proliferated in comparison to LTA-untreated ones. At this context, mTAP and CMC showed similar cytotoxicity than the observed for LTA-untreated cells, while mCMC was shown cytotoxic at 7 days only for LTA-primed APC. Comparing the medications, mTAP was more cytotoxic than CMC and mCMC. Conclusion mTAP showed higher cytotoxicity than CMC and mCMC and the effect of topic antimicrobials might differ when tested against apical papilla cells under physiological or activated conditions.

Humans , Male , Female , Adolescent , Teichoic Acids/toxicity , Lipopolysaccharides/toxicity , Enterococcus faecalis/chemistry , Tooth Apex/cytology , Dental Papilla/cytology , Anti-Bacterial Agents/toxicity , Root Canal Irrigants/toxicity , Time Factors , Calcium Hydroxide/toxicity , Calcium Hydroxide/chemistry , Ciprofloxacin/toxicity , Ciprofloxacin/chemistry , Cefaclor/toxicity , Cefaclor/chemistry , Cell Survival/drug effects , Cells, Cultured , Reproducibility of Results , Analysis of Variance , Tooth Apex/drug effects , Dental Papilla/drug effects , Metronidazole/toxicity , Metronidazole/chemistry , Anti-Bacterial Agents
J. appl. oral sci ; 21(1): 43-47, 2013. tab, graf
Article in English | LILACS, BBO | ID: lil-684994


Objectives: The aim of the present study was to investigate the effects of root canal sealers on the cytotoxicity of 3T3 fibroblasts during a period of 5 weeks. Material and Methods: Fibroblasts (3T3, 1×105 cells per well) were incubated with elutes of fresh specimens from eight root canal sealers (AH Plus, Epiphany, Endomethasone N, EndoREZ, MTA Fillapex, Pulp Canal Sealer EWT, RoekoSeal and Sealapex) and with elutes of the same specimens for 5 succeeding weeks after immersing in simulated body fluid. The cytotoxicity of all root canal sealers was determined using the MTT assay. Data were analyzed using ANOVA and Tukey's test. Results: RoekoSeal was the only sealer that did not show any cytotoxic effects (p<0.05). All the other tested sealers exhibited severe toxicity initially (week 0). MTA Fillapex remained moderately cytotoxic after the end of experimental period. Toxicity of the other tested sealers decreased gradually over time. The evaluated root canal sealers presented varying degrees of cytotoxicity, mainly in fresh mode.Conclusions: RoekoSeal had no cytotoxic effect both freshly mixed and in the other tested time points. MTA Fillapex was associated with significantly less cell viability when compared to the other tested root canal sealers.

Animals , Mice , /drug effects , Root Canal Filling Materials/toxicity , Biocompatible Materials/toxicity , Calcium Hydroxide/toxicity , Cell Survival/drug effects , Composite Resins/toxicity , Drug Combinations , Dental Cements/toxicity , Dexamethasone/toxicity , Epoxy Resins/toxicity , Formaldehyde/toxicity , Hydrocortisone/toxicity , Salicylates/toxicity , Time Factors , Thymol/analogs & derivatives , Thymol/toxicity
Braz. oral res ; 26(5): 424-430, Sept.-Oct. 2012. graf, tab
Article in English | LILACS | ID: lil-649362


The aim of this work was to evaluate the effects of different times of extraction on the cytotoxicity of six representatives of different root canal sealer groups-Real Seal SE, AH Plus, GuttaFlow, Sealapex, Roth 801, and ThermaSeal Plus-with human gingival fibroblasts. The materials were prepared according to manufacturers' specifications, and were incubated in culture medium (DMEM) at 37ºC for 1, 7, 14, 21, and 28 days, with daily washing, to simulate periodontal ligament clearance. Human fibroblasts were exposed to the final extracts at 24 hours, and cell viability was determined by MTT assay, with exposure to unconditioned DMEM as a negative control. Statistical analysis comparing cytotoxicities at each exposure time was performed by ANOVA with Scheffé adjustment for multiple comparisons at a 95% confidence level. Results indicated that GuttaFlow was significantly less cytotoxic than all other sealers (p < 0.05) at 1 day of extraction. After 7 days of extraction, cell viability for GuttaFlow was significantly increased as compared with that of all groups except sealer AH Plus. At day 14, cytotoxicity of Sealapex was significantly higher than that of all other sealers (p < 0.05). At days 21 and 28, there were no significant differences in cytotoxicity among sealer groups. All materials presented some level of cytotoxicity to fibroblasts, while GuttaFlow was the least cytotoxic sealer tested. However, the cytotoxicity of all materials seemed to decrease similarly in a time-dependent manner.

Humans , Fibroblasts/drug effects , Root Canal Filling Materials/toxicity , Analysis of Variance , Cell Survival , Cells, Cultured , Calcium Hydroxide/toxicity , Composite Resins/toxicity , Drug Combinations , Dimethylpolysiloxanes/toxicity , Epoxy Resins/toxicity , Gutta-Percha/toxicity , Materials Testing , Salicylates/toxicity , Time Factors
Rev. clín. pesq. odontol. (Impr.) ; 6(2): 135-140, maio-ago. 2010. graf
Article in English | LILACS, BBO | ID: lil-617376


OBJECTIVE: Endodontic irrigants solutions with antibacterial activity have been used in treatment of teeth with infected root canals; however, these solutions can irritate periapical tissues. The aim of this study was to evaluate the cytotoxity and genotoxicity of different endodontic irrigants solutions – sodium hypochlorite (1% and 2%), calcium hydroxide (0.2%), and HCT20 in human KB cells. MATERIAL AND METHOD: Cells were incubated with solutions for 2 and 24 hours. The cell viability was assessed after the trypan blue exclusion and the frequency of cell death mechanism (apoptotic or necrotic) was determined by acridine orange/ethidium bromide fluorescent dyeing test. The genotoxicity effects were assessed by the micronucleus assays. RESULTS AND DISCUSSION: The results showed that Ca(OH)2 alone or in combination with tergentol (HCT20), and NaOCl induced cytotoxicity in KB causing death cells by apoptosis. The micronuclei test showed that KB treated with NaOCl (1%) present an increase in the frequency of micronucleus compared to the control group.

OBJETIVO: Soluções irrigadoras com atividade antibacteriana têm sido usadas no tratamento de dentes com canais radiculares infectados; entretanto, essas soluções podem irritar os tecidos periapicais. O objetivo deste estudo foi avaliar a citotoxicidade e genotoxicidade de diferentes soluções irrigadoras – hipoclorito de sódio (1% e 2%), hidróxidode cálcio (0,2 %) e HCT em células humanas KB. MATERIAL E MÉTODO: As células foram incubadas em soluções por 2 e 24 horas. A viabilidade celular foi determinada após exclusão do tripan blue e a frequência de mecanismo de morte celular (apoptótica ou necrótica) foi determinada pelo teste acridine Orange/ethidium bromide fluorescen dyeing. Os efeitos de genotoxicidade foram determinados pelo ensaio de micronúcleos. RESULTADOS E DISCUSSÃO: Os resultados demonstraram que o Ca(OH2), isoladamente ou em combinação com Tergentol™ (HCT20) e NaOCl, induziram citotoxicidade em KB, causando morte celular por apoptose. O teste de micronúcleos demonstrou que KB tratado codm NaOCl (1%) apresentou aumento na frequência de microdnúcleos quando comparadocom o grupo controle.

Calcium Hydroxide/toxicity , Disinfectants/toxicity , KB Cells/drug effects , Root Canal Irrigants/toxicity , Sodium Hypochlorite/toxicity , Analysis of Variance , Cell Survival , DNA Damage/drug effects , Genotoxicity , Micronucleus Tests , Microscopy, Fluorescence , Time Factors
J. appl. oral sci ; 17(5): 457-461, Sept.-Oct. 2009. ilus, graf
Article in English | LILACS | ID: lil-531397


OBJECTIVE: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. MATERIAL AND METHODS: Undiluted (crude extract) and diluted extracts to 10 percent, 1 percent, 0.1 percent, 0.01 percent, 0.001 percent and 0.0001 percent of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5 percent significance level. RESULTS: Crude extract of AH Plus and Fill Canal killed approximately 90 percent of THP-1 cells versus 36 percent of THP-1 cells killed by L&C crude extract (p<0.05). Ten-fold dilutions of L&C, Fill Canal and AH Plus killed 24, 35 and 61 percent of THP-1 cells (p<0.05), respectively. Dilutions lesser than 1 percent caused minimal cell death as compared to the control groups (p>0.05), except for L&C 1 percent extract. CONCLUSIONS: The results revealed that the L&C paste crude extract was less cytotoxic to THP-1 cells than AH Plus or Fill Canal crude extracts.

Humans , Calcium Hydroxide/toxicity , Root Canal Filling Materials/toxicity , Barium Sulfate/toxicity , Bismuth/toxicity , Borates/toxicity , Cell Count , Cell Line, Tumor , Cell Death/drug effects , Cell Survival/drug effects , Coloring Agents , Drug Combinations , Epoxy Resins/toxicity , Eugenol/toxicity , Materials Testing , Monocytes/drug effects , Resins, Synthetic/toxicity , Trypan Blue , Zinc Oxide-Eugenol Cement/toxicity , Zinc Oxide/toxicity
Braz. dent. j ; 20(1): 22-26, 2009. graf, ilus
Article in English | LILACS | ID: lil-513909


This study compared the cytotoxicity of an experimental epoxy-resin and calcium hydroxide-based cement (MBPc), gray mineral trioxide aggregate (MTA) and white mineral trioxide aggregate (WMTA) using the agar overlay method with neutral red dye. L929 cells were seeded into 6-well culture plates where 48-h set test materials were placed on the agar overlay, in triplicate. Teflon and natural rubber served as negative and positive controls. After an incubation period of 24 h at 37ºC in a humidified atmosphere of 5 percent CO2 in air, a discolored area around the samples and the positive controls could be observed and measured per quadrant. The mean values were compared and converted into grades to classify the results according to the table of cytotoxicity grades according to the Standard Operating Procedures (SOP) of the Oswaldo Cruz Foundation, Brazil. The nonviable cell areas and the morphological changes in the cells were observed with an inverted microscope. The results showed grade 1 (slight) for the two types of MTA (p>0.05) and grade 2 (mild) for the MBPc (p<0.001). All samples met the requirements of the test as none of the cultures showed reactivity higher than grade 2.

O objetivo deste estudo foi comparar a citotoxicidade de um cimento experimental à base de resina epóxica e hidróxido de cálcio (MBPc), do agregado trióxido mineral (MTA) cinza e do MTA branco, utilizando o ensaio de difusão em agar com o corante vermelho neutro. Células L929 foram semeadas em placas de 6 poços e sobre elas a camada de agar, onde foram colocados os materiais endurecidos por 48 h, em triplicata, além de teflon como controle negativo e látex como controle positivo. Após 24 h em estufa umidificada a 37ºC com 5 por cento CO2, um halo claro se formou ao redor das amostras e dos controles positivos. As medidas foram tomadas, por quadrante, e as médias foram comparadas e convertidas em graus para qualificar os resultados, de acordo com a tabela de grau de citotoxicidade do POP/FIOCRUZ. As zonas de inibição e as alterações da morfologia celular foram avaliadas sob microscópio invertido. Os resultados revelaram grau 1 (leve) para os dois tipos de MTA (p>0,05) e grau 2 (branda) para o MBPc (p<0,001). Todas as amostras foram consideradas satisfatórias, pois nenhuma cultura exposta aos cimentos revelou toxicidade superior ao grau 2.

Animals , Mice , Fibroblasts/drug effects , Resin Cements/toxicity , Root Canal Filling Materials/toxicity , Tooth Injuries/therapy , Tooth Root/injuries , Aluminum Compounds/toxicity , Calcium Compounds/toxicity , Calcium Hydroxide/toxicity , Cell Survival/drug effects , Drug Combinations , Dental Instruments/adverse effects , Epoxy Resins/toxicity , L Cells , Oxides/toxicity , Resin Cements/chemistry , Root Canal Filling Materials/chemistry , Root Canal Preparation/adverse effects , Root Canal Preparation/instrumentation , Silicates/toxicity
Braz. dent. j ; 20(2): 99-106, 2009. ilus
Article in English | LILACS | ID: lil-524516


This study was evaluated the response of subcutaneous connective tissue of isogenic mice to calcium hydroxide-based pastes with chlorhexidine digluconate (CHX). Seventy isogenic male BALB/c mice aged 6-8 weeks and weighing 15-20 g were randomly assigned to 8 groups. The animals received polyethylene tube implants as follows: Groups I, II, and III (n=10) - Calen® paste mixed with 0.4 percent CHX (experimental paste; Calen/CHX) for 7, 21, and 63 days, respectively; Groups IV, V, and VI (n=10) - UltraCal™ paste mixed with 2 percent CHX (experimental paste supplied by Ultradent Products Inc.; Ultracal/CHX) for 7, 21, and 63 days, respectively; and Groups VII and VIII (n=5): empty tube for 7 and 21 days, respectively. At the end of the experimental periods, the implants were removed together with the surrounding tissues (skin and subcutaneous connective tissue). The biopsied tissues were subjected to routine processing for histological analysis. Using a descriptive analysis and a four-point (0-3) scoring system, the following criteria were considered for qualitative and quantitative analysis of the tissue around the implanted materials: collagen fiber formation, tissue thickness and inflammatory infiltrate. A quantitative analysis was performed by measuring the thickness (µm), area (µm²) and perimeter (µm) of the reactionary granulomatous tissue formed at the tube ends. Data were analyzed statistically by the Kruskal-Wallis test and Dunn's post-test (á=0.05). Calen/CHX showed biocompatibility with the subcutaneous and reactionary tissues, with areas of discrete fibrosis and normal conjunctive fibrous tissue, though without statistically significant difference (p>0.05) from the control groups. In Groups I to III, there was a predominance of score 1, while in Groups IV to VI scores 2 and 3 predominated for all analyzed parameters. UltraCal/CHX, on the other hand, induced the formation of an inflammatory infiltrate and abundant exudate, ...

O objetivo do presente estudo foi avaliar a resposta do tecido conjuntivo subcutâneo de camundongos isogênicos frente a pastas à base de hidróxido de cálcio, associadas ao digluconato de clorexidina (CHX). Setenta camundongos isogênicos BALB/c machos, com 6-8 semanas e pesando 15-20 g foram aleatoriamente divididos em 8 grupos. Os animais receberam implantes de tubos de polietileno contendo: Grupos I, II e III (n=10) - pasta Calen® associada à CHX a 0,4 por cento (Calen/CHX), por 7, 21 e 63 dias, respectivamente; Grupos IV, V e VI (n=10) - pasta UltraCal™ associada à CHX a 2 por cento (pasta experimental fornecida pela Ultradent Products Inc.; Ultracal/CHX), por 7, 21 e 63 dias, respectivamente; e Grupos VII e VIII (n=5) - tubo de polietileno vazio, por 7 e 21 dias, respectivamente. Decorridos os períodos experimentais, os implantes foram removidos juntamente com os tecidos circundantes (pele e tecido conjuntivo). Os tecidos foram submetidos ao processamento histotécnico de rotina, para análise histopatológica. Empregando um sistema de escores, os seguintes critérios foram considerados para a análise qualitativa e quantitativa: fibrosamento, espessura tecidual e infiltrado inflamatório. Foi efetuada, também, a análise quantitativa da medida da espessura (µm), área (µm²) e perímetro (µm) do tecido granulomatoso reacional formado na abertura dos tubos. Os dados obtidos foram submetidos à análise estatística, empregando o teste de Kruskal-Wallis e o pós-teste de Dunn. O nível de significância adotado foi de 5 por cento. Os resultados demonstraram biocompatibilidade da pasta Calen associada à CHX a 0,4 por cento com o tecido adjacente, com fibrosamento discreto, assim como tecido conjuntivo normal, sem diferença estatística significante com os controles (p>0,05). Nos Grupos I, II e III houve predominância do escore 1, enquanto que nos Grupos IV, V e VI houve predominância dos escores 2 e 3, em todos os parâmetros analisados. Em relação ...

Animals , Male , Mice , Anti-Infective Agents, Local/toxicity , Calcium Hydroxide/toxicity , Chlorhexidine/analogs & derivatives , Root Canal Filling Materials/toxicity , Subcutaneous Tissue/drug effects , Chlorhexidine/toxicity , Drug Combinations , Mice, Inbred BALB C , Random Allocation
Braz. dent. j ; 20(2): 118-121, 2009. ilus
Article in English | LILACS | ID: lil-524518


The aim of this study was to evaluate the cytotoxicity of root canal irrigating solutions containing calcium hydroxide and sodium lauryl sulphate on fibroblasts derived from L929 cell line. Saturated calcium hydroxide aqueous solution (CH), sodium lauryl sulphate (SLS) and SLS associated with calcium hydroxide (HCT20) were diluted with sterile distilled water at 50 percent, 20 percent, 10 percent and 5 percent concentrations. Minimum essential medium (MEM) served as the control group. The cytotoxicity of the solutions was evaluated on L929 mouse fibroblast cell line, at 4 and 24 h of contact time by the 51Cr radiotracer method. Data were compared and statistical inferences were made with the chi-square test. In all analysis, significance level was set at 5 percent. CH and HCT20 showed toxicity at 50 percent concentration, while at concentrations lower than 50 percent these solutions showed cell tolerance. SLS was cytotoxic at all concentrations. In conclusion, the association of calcium hydroxide and SLS (HCT20) combines the beneficial properties of these solutions and was not harmful to the fibroblast cell line, seeming to be a suitable endodontic irrigating solution.

O objetivo desta pesquisa foi avaliar a citotoxicidade de soluções irrigadoras de canais radiculares contendo hidróxido de cálcio e lauril sulfato de sódio em linhagem de fibroblastos L929. Solução aquosa saturada de hidróxido de cálcio, lauril sulfato de sódio e HCT20 (lauril sulfato de sódio e hidróxido de cálcio) foram diluídos em água destilada em concentrações de 50 por cento, 20 por cento, 10 por cento e 5 por cento. O grupo controle foi representado por meio de cultura de células (MEM - minimum essential medium). A citotoxicidade das soluções sobre os fibroblastos foi avaliada em 4 e 24 h de contato, pelo método do cromo radioativo. Os resultados foram analisados estatisticamente pelo teste do qui-quadrado. Em todas as análises, o intervalo de confiança referente às médias entre os grupos foi estabelecido em 95 por cento. As soluções saturadas de hidróxido de cálcio e o HCT20 apresentaram toxicidade nas concentrações de 50 por cento. O lauril sulfato de sódio foi tóxico em todas as concentrações. As soluções de hidróxido de cálcio em concentrações menores que 50 por cento apresentaram tolerância celular, assim como combinadas ao lauril sulfato de sódio. Tal comportamento não foi observado na solução pura de lauril sulfato de sódio em todas as concentrações.

Animals , Mice , Root Canal Irrigants/toxicity , Calcium Hydroxide/toxicity , Fibroblasts/drug effects , L Cells , Sodium Dodecyl Sulfate/toxicity
Bauru; s.n; 2004. 127 p. ilus.
Thesis in Portuguese | LILACS, BBO | ID: lil-407605


O principal objetivo deste trabalho foi estabelecer, através da definição da dose TD50 (concentração que causa a morte de 50% das células) uma escala de toxicidade dentre diversas substâncias comumente utilizadas em pulpotomias de dentes decíduos. Foram utilizadas como substâncias-teste o formocresol concentrado (FC), formocresol diluído a 1:5 (FCD), solução de sulfato férrico a 15,5% (SF), hidróxido de cálcio P.A. (HC) e o agregado trióxido mineral (MTA). Fibroblastos Balb-c 3T3 foram semeados em placas de cultura de 24 poços na concentração de 3x '10 POT. 4' células por poço. Diluições variadas dos extratos das substâncias-teste, obtidos de acordo com normas da ISO 10993-12, foram colocadas em contato com as células por 24 horas para a determinação da TD50, dose tóxica para 50% das células quando comparadas com grupo controle negativo (células não expostas aos extratos). Os ensaios colorimétricos de redução do MTT e captação do corante Vermelho Neutro foram realizados na TD50 das substâncias-teste. O tratamento estatístico dos resultados foi feito através dos testes de Análise de Variância (ANOVA), Dunnet e Bartlett, com p < 0,05. Baseado na definição da TD50, pode ser estabelecida a escala de citotoxicidade em ordem decrescente, a saber: FC > FCD > SF > HC > MTA. Os ensaios para a determinação da capacidade de redução do MTT e incorporação de VN demonstraram que a citotoxicidade dos materiais afeta, primordialmente, a função mitocondrial em detrimento do efeito mais discreto na integridade da membrana. Concluímos que a determinação da dose TD50 utilizando a metodologia proposta pela ISO 10993-12 permite o estabelecimento de uma escala de potência adequada para a ordenação de compostos terapêuticos em função de sua toxicidade. Esta padronização poderá facilitar a comparação entre diversos materiais auxiliando na investigação de novas drogas. Ainda, é possível concluir que os materiais testados e utilizados para a pulpotomia de dentes decíduos têm sua toxicidade mediada principalmente por alterações da função mitocondrial das células

In Vitro Techniques , Pulpotomy , Toxic Substances , Ferric Sulfate , Fibroblasts , Formocresols/toxicity , Calcium Hydroxide/toxicity , Tooth, Deciduous
ROBRAC ; 6(22): 9-13, dez. 1997. ilus
Article in Portuguese | LILACS, BBO | ID: lil-235781


Os compômeros ou ionocompósitos säo materiais restauradores híbridos, derivados dos cimentos de ionômero de vidro convencionais com pequenas adiçöes de resinas compostas fotoativada, exibindo propriedades intermediárias aos dois materiais, com algumas características superiores aos cimentos ionoméricos. O objetivo do trabalho foi avaliar de forma comparativa a biocompatibilidade do compômeros Variglass VLC e do cimento de hidróxido de cálcio - Dycal. Para isto ambos materiais foram implantados no tecido conjuntivo subcutâneo de ratos, onde permaneceram pelos períodos de 7, 15, 30 e 60 dias. Variglass VLC provocou no primeiro período de células inflamatórias ao 7 dias, sendo que a área reacional atingiu amplitude avaliaçäo, moderada - intensa reaçäo inflamatória, onde a área reacional junto à abertura tubular tinha amplitude média de 3,822 mm, decrescendo com o decorrer do período para 0,506 mm. Já o grupo controle (Dycal) apresentou discreta quantidade média de 1,118 mm. Houve regressäo do quadro reacional com o decorrer dos períodos, sendo que aos 60 dias a amplitude média era de 0,347 mm. Conclui-se que o Variglass VLC foi mais irritante que o Dycal, porém ambos materiais apresentaram biocompatibidade aceitável

Animals , Male , Adult , Glass Ionomer Cements/toxicity , Composite Resins/toxicity , Connective Tissue/anatomy & histology , Calcium Hydroxide/toxicity , Biocompatible Materials/toxicity , Dental Pulp Necrosis , Dental Leakage/microbiology , Dental Pulp/pathology
ROBRAC ; 6(22): 28-31, dez. 1997. tab
Article in Portuguese | LILACS, BBO | ID: lil-235786


Com a finalidade de avaliar o potencial citotóxico de algumas soluçöes utilizadas com auxiliares no preparo biomecânico dos sistema de canais radiculares, testou-se os efeitos hemolíticos e hemoglobinolíticos in vitro, de tais soluçöes através de diluiçäo em soro fisiológico normal. Foram testadas as seguintes soluçöes: soluçäo de hidróxido de cálcio a 0,2 por cento (água de cal), Tergentol, associaçäo de água de cal e Tergentol em duas proporçöes (HcT10 e Hct20), líquido de Dakin, soluçäo de Milton, soluçäo de Labarraque e soda clorada. Para tal, após a padronizaçäo da fragilidade globular das hemácias através de diluiçäo volume a volume de sangue venoso com soluçäo de Alsever modificada e armazenamento em geladeira por 4 dias, diluiu-se as soluçöes teste em concentraçöes decrescente de razäo variável em soro fisiológico puro, após o que procedeu-se o contato célula-material por 30 minutos, centrifugaçäo de tubos e leitura das densidades ópticas em Espectrofotômetro Espectra I no comprimento de onda de 545 mm. Após a ordenaçäo dos dados, calculou-se as médias para n=2 e os respectivos desvios padröes (DP) para cada diluiçäo das soluçöes. Os resultados permitiram concluir tratar-se de um método válido para tal finalidade por ser quantitativo, reprodutivo e de fácil execuçäo; que a adiçäo de um agente tênsio-ativo (tergentol) reduz significativamente o potencial hemolítico e hemoglobinolítico da água de cal; que o tipo e a concentraçäo das soluçöes säo determinantes no seu potencial hemolítico; e que as soluçöes de hipoclorito de sódio säo extremamente hemolítica e hemoglobinolíticas e, consequentemente, altamente citotóxicas

Dental Pulp Cavity/blood supply , Detergents , Calcium Hydroxide/toxicity , In Vitro Techniques , Root Canal Irrigants , Root Canal Therapy , Sodium Hypochlorite/toxicity , Dental Pulp Cavity , Root Canal Preparation , Solutions
Bauru; s.n; 1996. 142 p. tab, graf.
Thesis in Portuguese | LILACS, BBO | ID: lil-250288


A citotoxicidade de cinco cimentos obturadores de canais radiculares, à base de hidróxido de cálcio (Sealapex, CRCS, Apexit e Sealer 26), e à base de óxido de zinco e eugenol (Fill Canal), foi avaliada em culturas de macrófagos peritoniais de camundongos. Utilizaram-se duas metodologias em culturas de macrófagos. A primeira relacionada à alteraçäo morfológica das células em contato com os cimentos após presa e a segunda, à liberaçäo de H2O2 pelas células em contato com os cimentos solubilizados. Os resultados permitiram concluir, revelando as limitaçöes metodológicas, que: 1) Na avaliaçäo da citotoxicidade através da alteraçäo morfológica dos macrófagos frente aos cimentos endodônticos, os mais citotóxicos foram em ordem crescente Fill Canal, CRCS, Sealer 26, Apexit e Sealapex. 2) Na avaliaçäo da resposta celular bioquímica dos macrófagos frente a esses cimentos, tendo-se como parâmetro o nível de H2O2 no meio de cultura dessas células, os mais citotóxicos, em ordem crescente foram CRCS, Sealapex, Apexit, Fill Canal e Sealer 26. 3) A avaliaçäo comparativa entre as metodologias mostrou a viabilidade de ambas como método de avaliaçäo citotóxica entre materiais de solubilidade semelhante

Animals , Male , Female , Infant , Mice , Dental Cements/toxicity , Macrophages, Peritoneal/cytology , Zinc Oxide-Eugenol Cement/toxicity , Calcium Hydroxide/toxicity , Biocompatible Materials/toxicity , Dental Materials/toxicity , Hydrogen Peroxide/toxicity