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1.
Braz. J. Pharm. Sci. (Online) ; 58: e18373, 2022. tab
Article in English | LILACS | ID: biblio-1364428

ABSTRACT

Abstract The aim of this study was to determine antimicrobial activities of Alchemilla mollis, Alchemilla persica as well as ellagic acid and miquelianin against Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans by using microbroth dilution method and anti-inflammatory activity by using human red blood cell (HRBC) membrane stabilization method. Microbroth dilution method was used to determine the antimicrobial activities. Extracts possessed activity having MIC values of 2.5-5-10mg/ mL, compounds possessed activity having MIC values of 1.25-2.5-4-5mg/mL. A.mollis aerial parts displayed the highest anti-inflammatory activity (IC50=1.22±0.07mg/mL). Ellagic acid and miquelianin were also determined as anti-inflammatory agents with 0.57±0.01mg/mL and 1.23±0.02mg/mL IC50 values, respectively. Total phenolic content and tannin content of the A.mollis and A.persica were determined as 357.00±75.80mg, 282.50±28.70mg PGE/g plant material and 18.02%, 18.63% respectively according to the method described by European Pharmacopoeia. Ellagic acid, miquelianin and catechin were analyzed by HPLC. The highest catechin content was detected in A.persica roots (6.69±0.05g/100g plant material). A.mollis aerial parts contain higher miquelianin (0.39±0.02g/plant material) and ellagic acid (1.56±0.01g/ plant material) than A.persica.


Subject(s)
Alchemilla/classification , Staphylococcus aureus , Bacillus subtilis , Candida albicans , Chromatography, High Pressure Liquid/methods , Dilution/methods , Ellagic Acid/pharmacology , Membranes , Anti-Inflammatory Agents
2.
Braz. dent. sci ; 25(1): 1-14, 2022. tab, ilus
Article in English | LILACS, BBO | ID: biblio-1361900

ABSTRACT

Objetivo: O objetivo do presente estudo foi avaliar a eficácia da adição de nanopartículas de óxido de zircônio (ZrO2), óxido de titânio (TiO2) e óxido de sílica (SiO2) a um material de revestimento macio curado a frio na adesão de Candida albicans (CA). Material e Método: Cinquenta e quatro pacientes foram selecionados e divididos em três grupos de acordo com a modificação do revestimento com nanopartículas de ZrO2, TiO2 e SiO2 (18 cada). Cada paciente recebeu prótese total maxilar com três cavidades, as cavidades foram revestidas com forro macio curado a frio modificado com diferentes concentrações (0%, 3% e 7%) de nanopartículas de óxido metálico. Nos dias 14 e 28, as trocas foram retiradas do local de realinhamento e imediatamente cultivadas para avaliação fúngica. O número de colônias foi contado, os dados coletados e explorados para normalidade usando o teste de Shapiro-Wilk e a transformação logarítmica da contagem de CA foi realizada. ANOVA para medidas repetidas e de uma via (one-way) foram usados, seguidos por teste de Tukey (HSD). O teste t independente foi usado para comparar as contagens de CA em diferentes períodos. Resultados: A adesão do CA foi significativamente diminuída pela adição de nanopartículas de ZrO2, TiO2 e SiO2 em comparação com o grupo controle, também a cobertura antifúngica aumentou com o aumento da concentração de nanopartículas (p <0,005). A maior contagem de CA foi identificada no grupo SiO2 seguido por ZrO2, enquanto TiO2apresentou a menor contagem de CA (p <0,001). Conclusão: Adição de diferentes nanopartículas; ZrO2, TiO2 e SiO2para revestimento macio curado a frio é um método eficaz para reduzir a adesão de CA (AU)


Objective: The aim of the current study was to evaluate the efficacy of addition of zirconium oxide (ZrO2), titanium oxide (TiO2), and silica oxide (SiO2) nanoparticles to cold-cured soft liner on adhesion of Candida albicans (CA). Material and Methods: Fifty-four patients had been selected and divided into three groups according to the modification of soft liner with ZrO2, TiO2, and SiO2 nanoparticles (18 each of). Each patient received maxillary complete denture having three cavities, the cavities were lined using cold cured soft liner modified with different concentration (0%, 3%, and 7%) of metal oxide nanoparticles. On days 14 and 28, swaps were taken out from relining site and immediately cultured for fungal evaluation. The number of colonies were counted, data collected and explored for normality using Shapiro-Wilk test, logarithmic transformation of CA count was performed. Repeated and one-way ANOVA were used followed by Tukey HSD. Independent-t test used to compare between CA counts at different periods. Results: The CA adhesion was significantly decreased by the addition of ZrO2, TiO2 and SiO2 nanoparticles in comparison with control group, also the antifungal coverage increased with nanoparticles concentration increased (P<0.005). The highest CA count was identified in group SiO2 followed by ZrO2, while TiO2 showed the lowest CA count (P <0.001). Conclusion: Addition of different nanoparticles; ZrO2, TiO2 and SiO2 to cold-cured soft liner is an effective method for reducing CA adhesion. (AU)


Subject(s)
Humans , Candida albicans , Denture Liners , Nanoparticles , Antifungal Agents
4.
Con-ciencia (La Paz) ; 9(1): 1-17, jun. 2021. ilus., tab
Article in Spanish | LILACS | ID: biblio-1284388

ABSTRACT

INTRODUCCIÓN: el aumento de la incidencia de las micosis ha generado la necesidad de desarrollar técnicas in vitro para el estudio de la susceptibilidad a los antifùngicos; El documento CLSI M27-A2 es el método de referencia para estudios de sensibilidad en levaduras. No obstante, este no subsana las necesidades de rutina de los laboratorios, principalmente por ser laboriosos; en consecuencia, métodos alternativos surgen ante la necesidad de contar con técnicas más sencillas, una de ellos es el ATB FUNGUS 3 que permite determinar la sensibilidad de Candida frente a diferentes antifùngicos. OBJETIVO: validar el método comercial ATB FUNGUS 3, frente al método de referencia M27-A2, con el fin de conocer su valor diagnóstico. MATERIAL Y MÉTODOS: se determinó la eficacia del método a través de parámetros de test diagnóstico; además, se evaluó la sensibilidad de 50 cepas de Candida albicans frente a Fluconazol (FLZ) e Itraconazol (ITZ) mediante el método comercial y el de referencia. RESULTADOS: se encontró que el ATB - FUNGUS 3 presenta una especificidad para FLZ de 100%, sensibilidad de 91%, valor predictivo positivo (VPP) de 56%, valor predictivo negativo (VPN) de 100%, con una eficacia diagnóstica de 92%, calculados para un intervalo de confianza (IC) de 95%; para ITZ la especificidad y sensibilidad fue de 88 % y 90% respectivamente, con un VPP de 64%, un VPN de 97%, eficacia diagnóstica de 90%, IC 95%. Para las pruebas de concordancia, el índice Kappa para FLZ e ITZ fue de 0,67 y 0,68 respectivamente. La prueba de Likelihood ratio para el FLZ fue (LR+) de 11,25 mientras que el (LR-) fue 0; para el ITZ (LR+) de 9,19 y el (LR-) fue 0,14. Reproducibilidad de 90 % (FLZ) y 85% (ITZ). CONCLUSIONES: el ATB FUNGUS 3, es una técnica rápida, de fácil realización y reproducible; pero el desempeño global de la técnica, sugiere que aún no es confiable para el diagnóstico en laboratorios, debido a los valores bajos obtenidos en los VPP, que indican que se podría derivar en errores al momento de determinar una cepa como sensible o resistente, punto importante al momento de decidir la conducta terapéutica.


INTRODUCTION: the higher incidence of mycoses has generated the need to develop in vitro techniques for susceptibility study to antifungal agents. CLSI M27-A2 is a reference method for yeast susceptibility studies. However, this method does not meet the needs of routine laboratories because it is difficult to follow all the processes. Consequently, alternative methods arise due to the need for simpler techniques. Then, one of them is ATB FUNGUS 3 which allows determining Candida's sensitivity to different antifungal agents. OBJECTIVE: validate the commercial method ATB FUNGUS 3 compared with the reference method M27-A2 in order to know its diagnostic value. MATERIAL AND METHODS: efficacy was determined by diagnostic test parameters. Moreover, sensitivity of 50 strains of Candida albicans at Fluconazole (FLZ) and Itraconazole (ITZ) was evaluated by the commercial and reference methods. RESULTS: ATB - FUNGUS 3 presents a specificity for FLZ of 100%, sensitivity of 91%, positive predictive value (PPV) 56%, negative predictive value (NPV) 100% with a diagnostic efficacy of 92%, calculated for a 95% confidence interval (CI). For ITZ, the specificity and sensitivity were 88% and 90% respectively, with a PPV 64%, a NPV 97% with a diagnostic efficacy of 90%, 95% CI. For the concordance tests, the Kappa index for FLZ and ITZ was 0.67 and 0.68 respectively. The Likelihood ratio test for FLZ was (LR +) of 11.25 while the (LR-) was 0; for ITZ (LR +) of 9.19 and the (LR-) was 0.14. Reproducibility of 90% (FLZ) and 85% (ITZ). CONCLUSIONS: the ATB FUNGUS 3 is a fast, easy and reproducible technique. However, the overall performance of the technique suggests that this method hasn't been reliable for diagnostic laboratory yet, because PPVS obtained low values. These PPVS indicate that it could lead to errors when determining a strain as sensitive or resistant. This is an important point when deciding the therapeutic conduct.


Subject(s)
Candida albicans , Antifungal Agents , Confidence Intervals , Diagnosis
5.
Rev. cuba. estomatol ; 58(2): e3026, 2021. graf
Article in Portuguese | LILACS, CUMED | ID: biblio-1289394

ABSTRACT

Introdução: Os fitoconstituintes são moléculas naturais que apresentam atividade antimicrobiana satisfatória e devem ser estudados quanto ao seu uso como novas substâncias para irrigação dos canais radiculares. Objetivo: Avaliar o efeito inibitório dos fitoconstituintes cinamaldeído e α-terpineol frente a biofilmes monoespécie e duoespécie de microrganismos envolvidos na infecção endodôntica. Métodos: Trata-se de um estudo experimental na área de microbiologia aplicada, in vitro, cego quanto às análises e randomizado. Foram selecionados os fitoconstituintes cinamaldeído e α-terpineol. A atividade antimicrobiana frente Candida albicans e Enterococcus faecalis foi avaliada por meio da análise da capacidade metabólica com o uso da resazurina e análise da viabilidade celular pelo plaqueamento. O meio de cultura e a clorexidina 1 porcento serviram de controle negativo e positivo, respectivamente. Resultados: Observou-se ausência de crescimento para exposição dos biofilmes nas concentrações de 10 e 5 mg/mL de ambos os fitoconstituintes. Na concentração de 2,5 mg/mL de terpineol, constatou-se crescimento somente nos biofilmes monoespécie de C. albicans e duoespécie. Já na concentração de 1mg/mL de terpineol e cinamaldeído, verificou-se crescimento para todos os biofilmes. Conclusão: O cinamaldeído e α-terpineol apresentaram atividade inibitória frente biofilmes monoespécie e duoespécie de Candida albicans e Enterococcus faecalis, nas concentrações de 10 e 5 mg/mL(AU)


Introducción: Los fitoconstituyentes son moléculas naturales que presentan actividad antimicrobiana satisfactoria y deben ser estudiados en cuanto a su uso como nuevas sustancias para irrigación de los canales radiculares. Objetivo: Evaluar el efecto inhibitorio de fitoconstituyentes cinamaldehído y α-terpineol frente a biopelículas monoespecies y duoespecies de microorganismos involucrados en la infección endodóntica. Métodos: Estudio experimental en el campo de la microbiología aplicada, in vitro, ciego al análisis y aleatorizado. Se seleccionaron los fitoconstituyentes cinamaldehído y α-terpineol. La actividad antimicrobiana frente Candida albicans y Enterococcus faecalis fue evaluada por medio del análisis de la capacidad metabólica con el uso de la resazurina y análisis de la viabilidad celular por el plaqueamiento. El medio de cultivo y la clorexidina 1 por ciento sirvieron de control negativo y positivo, respectivamente. Resultados: Se observó ausencia de crecimiento para exposición de las biopelículas en las concentraciones de 10 y 5 mg/mL de ambos fitoconstituyentes. En la concentración de 2,5 mg/mL de terpineol se constató crecimiento solo en los biofilmios monoespecies de C. albicans y duoespecies. En la concentración de 1 mg/mL de terpineol y cinamaldehído se verificó crecimiento para todas las biopelículas. Conclusiones: Cinamaldehído y α-terpineol presentaron actividad inhibitoria frente a biofilmes monoespecies y duoespecies de Candida albicans y Enterococcus faecalis, en las concentraciones de 10 y 5 mg/mL(AU)


Introduction: Phytoconstituents are natural molecules displaying satisfactory antimicrobial activity. Studies should be conducted about their use as new root canal irrigants. Objective: Evaluate the inhibitory effect of the phytoconstituents cinnamaldehyde and α-terpineol against mono- and duo-species biofilms of microorganisms involved in endodontic infection. Methods: An experimental applied microbiology blind randomized in vitro study was conducted. The phytoconstituents selected were cinnamaldehyde and α-terpineol. Antimicrobial activity against Candida albicans and Enterococcus faecalis was evaluated by metabolic capacity analysis with resazurin and cell viability analysis by the plaque. The culture medium and 1 percent chlorhexidine served as negative and positive controls, respectively. Results: An absence of growth was observed for exposure of the biofilms at concentrations of 10 and 5 mg/ml of both phytoconstituents. At a concentration of 2.5 mg/ml terpineol displayed growth only in the mono-species biofilms of C. albicans and duo-species biofilms. At a concentration of 1 mg/ml terpineol and cinnamaldehyde displayed growth in all biofilms. Conclusions: Cinnamaldehyde and α-terpineol displayed inhibitory activity against mono- and duo-species biofilms of Candida albicans and Enterococcus faecalis at concentrations of 10 and 5 mg/ml(AU)


Subject(s)
Humans , Biological Products/adverse effects , Candida albicans , Cell Survival , Biofilms , Enterococcus faecalis , Anti-Infective Agents/adverse effects
6.
Vitae (Medellín) ; 28(2): 1-8, 2021-05-18. Ilustraciones
Article in English | LILACS, COLNAL | ID: biblio-1363196

ABSTRACT

Background: "Dividivi" Caesalpinia coriaria (Jacq.) Willd fruits are traditionally used by the Wayuú community in La Guajira (Colombia) to treat oral and skin cavity diseases caused by bacteria and fungi. Streptococcus pyogenes is a gram-positive cocci of group A (beta-hemolytic) that is the cause of pharyngeal disease, scarlet fever, cellulitis, erysipelas, or toxic shock-like syndrome. Alternatively, Candida albicans is a yeast-like fungus that is a normal flora of the digestive tract, vagina, or skin folds; it has been known to be the root cause of opportunistic diseases such as diaper rash, oral and esophagus thrush, or vulvovaginitis. Objective: This study evaluated the antimicrobial activity of methanolic and ethanolic extracts of C. coriaria (Jacq.) Willddry fruits on S. pyogenes ATCC 12384andC. albicans ATTC 14053. Method: C. coriaria extracts were obtained from the Soxhlet method using two solvents (methanol and ethanol 98%) prepared from pulverized fruits. A phytochemical test and an antimicrobial activity assay were performed using the obtained extracts and tested using S. pyogenes ATCC 12384 and C. albicans ATTC 14053 strains. Results: A phytochemical profile was performed, examining the presence of bioactive metabolites (tannins, alkaloids, glycosides, saponins, and anthraquinones) from each extract. Antimicrobial susceptibility tests showed that the ethanolic extract inhibited S. pyogenes ATCC 12384,causing inhibition halos of 14.1 ± 0.1 mm and a Minimum Inhibitory Concentration (MIC) of 172 mg/ml, and C. albicans test shows inhibition halos of 16.1 ± 0.2 mm and MIC of 212 mg/ml. Additionally, the methanolic extract inhibited S. pyogenes with inhibition halos of 15.2 ± 0.2 mm and MIC of 152 mg/ml; no inhibitory effect was observed on C. albicans.Conclusion: This study revealed that C. coriaria has an antimicrobial effect on the tested species opening the field of its possible use as a therapeutic agent


Introducción: Los frutos del "Dividivi" Caesalpinia coriaria (Jacq.) Willd son usados tradicionalmente por la comunidad indígena Wayuú en La Guajira (Colombia) para el tratamiento de enfermedades de la cavidad bucal y cutáneas ocasionadas por bacterias y hongos. Streptococcuspyogenes es un coco grampositivo del grupo A (beta-hemolítico) que es la causa de enfermedad faríngea, escarlatina, celulitis, erisipela o síndrome tipo shock tóxico. Candida albicans es un hongo levaduriforme que es flora normal del tracto digestivo, la vagina o los pliegues de la piel; se sabe que es la causa principal de enfermedades oportunistas como la dermatitis del pañal, aftas bucales y esofágicas, o vulvovaginitis. Objetivo: El objetivo de este estudio fue evaluar la actividad antimicrobiana de extractos metanólicos y etanólicos de frutos secos sobre microorganismos patógenos específicamente S. pyogenes ATCC 12384yC. albicansATTC 14053. Método: A partir de frutos polverizados de C. coriaria, usando el método Soxlet, se evaluaron dos solventes (metanol y etanol al 98%), los cuales, fueron usados para estudiar su actividad antimicrobiana evaluando su efecto en cepas de S. pyogenes ATCC 12384 y C. albicans ATTC 14053. Resultados: Mediante un perfil fitoquímico se determinó la presencia de grupos de metabolitos secundarios con compuestos bioactivos (taninos, alcaloides, glucósidos, saponinas, y antraquinonas). Las pruebas de sensibilidad antimicrobiana mostraron que el extracto etanólico tuvo un efecto inhibidor sobre S. pyogenesATCC 12384 con halos de inhibición de 14.1 ± 0.1 mm y una concentración mínima inhibitoria (CMI) de 172 mg/mL, y sobre C. albicans se presentaron halos de inhibición de 16.1 ± 0.2 mm y CMI de 212 mg/mL, mientras que el extracto metanólico tuvo un efecto inhibidor sobre S. pyogenes con halos de inhibición de 15.2 ± 0.2 mm y CMI de 152 mg/mL no se observó efecto inhibidor sobre C. albicans. Conclusión: Este estudio demostró que C. coriaria tiene efecto antimicrobiano en las especies evaluadas, abriendo un campo de investigación en la evaluación de su uso como agente terapéutico


Subject(s)
Humans , Anti-Infective Agents , Skin Diseases , Streptococcus pyogenes , Candida albicans , Microbial Sensitivity Tests , Mouth Diseases
7.
Article in English | LILACS | ID: biblio-1284447

ABSTRACT

This paper described the chemical compositions and antimicrobial activity of the essential oils from the leaves and stem of Amomum rubidumLamxay & N. S. Lý, collected from Bidoup Nui Ba National Park, Lam Dong, Vietnam. The essential oils were obtained by hydrodisitllation method while antimicrobial activity was evaluetd by microdilution broth susceptibility assay. The main constituents of the leaf essential oil were identified as 1,8-cineole (37.7%), δ-3-carene (19.5%) and limonene (16.3%) while δ-3-carene (21.9%), limonene (17.8%) and ß-phellandrene (14.6%) dominated in the stem essentialoil. The leaf and stem essential oils displayed stronger inhibition of Pseudomonas aeruginosa with MIC of 25 µg/mLand 50 µg/mL respectively. The stem essential oil was active against Candida albicans (MIC, 50 µg/mL) while both essential oils inhibited the growth of Fusarium oxysporum (MIC 50 µg/mL). This is the first report on chemical constituents and antimicrobial activity of the essential oils of A. rubidum.


Este artículo describe la composición química y la actividad antimicrobiana de aceites esenciales de las hojas y el tallo de Amomum rubidum Lamxay & N. S. Lý recolectados del Parque Nacional Bidoup Nui Ba, Lam Dong, Vietnam. Los aceites esenciales se obtuvieron mediante el método de hidrodisitilación, mientras que la actividad antimicrobiana se evaluó mediante un ensayo de susceptibilidad de caldo de microdilución. Los principales componentes del aceite esencial de la hoja se identificaron como 1,8-cineol (37,7%), δ-3-careno (19,5%) y limoneno (16,3%), mientras que δ-3-careno (21,9%), limoneno (17,8 %) y ß-felandreno (14,6%) dominaron en el aceite esencial del tallo. Los aceites esenciales de hoja y tallo mostraron una inhibición más fuerte de Pseudomonas aeruginosa con un MIC de 25 µg/mL y 50 µg/mL, respectivamente. El aceite esencial del tallo fue activo contra Candida albicans (MIC, 50 µg/mL) mientras que ambos aceites esenciales inhibieron el crecimiento de Fusarium oxysporum (MIC 50 µg/mL). Este es el primer informe sobre los componentes químicos y la actividad antimicrobiana de los aceites esenciales de A. rubidum.


Subject(s)
Oils, Volatile/pharmacology , Amomum/chemistry , Anti-Infective Agents/pharmacology , Pseudomonas aeruginosa/drug effects , Candida albicans/drug effects , Oils, Volatile/chemistry , Microbial Sensitivity Tests , Distillation , Chromatography, Gas , Plant Stems , Plant Leaves , Monoterpenes/analysis , Fusarium/drug effects , Anti-Infective Agents/chemistry
8.
Rev. patol. trop ; 50(3)2021. ilus
Article in English | LILACS | ID: biblio-1292499

ABSTRACT

This study characterized and related yeasts of the genus Candida isolated from vaginal mucous membranes of women with lesions caused by high-risk HPV for cervical cancer. Forty-two women treated at the Lower Genital Tract Pathology Clinic of the University of São Paulo Medical School Hospital of Clinics were examined, with 30 high-grade (G1) uterine lesions with a mean age of 36.5 years ± 11. 1 and 12 with low grade (G2) uterine lesions with a mean age of 34.7 years ± 15.5. Clinical conditions and laboratory data on HPV were collected from patients' medical records; the socio-demographic data obtained from an appropriate questionnaire. For the study of association between the variables, Odds Ratio analysis was used from the STATA 13.1 program. Patients G1 had a higher prevalence for diabetes and the results indicated 27% prevalence of Candida spp. in vaginal mucosa, in G2 this was 33% in vaginal mucosa. Among the species found in vaginal mucosa of patients, Candida albicans was the most isolated with 88%, followed by C. tropicalis (8%) and C. glabrata (4%). The strains of C. albicans isolated from mucosa presented sensitivity to all antifungal agents tested, unlike the C. tropicalis strain isolated in G2 in vaginal mucosa, which presented a resistance profile to fluconazole. Thus, monitoring and supervision through clinical and laboratory testing of HPV patients is important, reinforcing the need for care, treatment and prevention of HPV-related infections and Candida spp.


Subject(s)
Humans , Female , Papillomaviridae , Candida albicans , Uterine Cervical Neoplasms , Mucous Membrane , Antifungal Agents
9.
Article in French | AIM, AIM | ID: biblio-1353553

ABSTRACT

Introduction : Les candidoses systémiques sont des affections graves responsables d'une mortalité élevée. L'objectif de ce travail est de décrire l'épidémiologie des candidoses systémiques dans les services à haut risque au CHU et au CAC de BATNA. Patients et méthodes : Il s'agit d'une étude prospective descriptive durant une période de trois ans (1er janvier 2016 au 31 décembre 2018). Les patients inclus sont ceux ayant au moins un prélèvement profond positif á Candida spp. Résultats : Un total de 69 cas de candidoses systémiques correspondant à 75 isolats et concernant 63 patients a pu être analysé. L'incidence globale était de 2,62 cas pour 1000 admissions. Les principaux motifs d'hospitalisation étaient les hémopathies malignes et le choc septique. La présence d'une colonisation ( 2 sites), une antibiothérapie á large spectre, d'un cathéter intra vasculaire, une corticothérapie, une chimiothérapie, une neutropénie étaient les facteurs de risque les plus retrouvés. L'analyse des souches isolées a montré la prédominance des espèces non albicans. L'index de colonisation ≥ 0,5 a été significativement associé au risque de candidose systémique. L'utilisation des Azolés a été associée á un taux de mortalité le plus élevé (19%). Le taux de mortalité est significativement élevé 51%. Conclusion : Les facteurs de risque et un index de colonisation ≥ 0,5 dans les services á haut risque constituent un facteur prédictif de candidose systémique. La prise en charge thérapeutique doit être instaurée pour réduire le taux de mortalité et éviter les complications liées á ces infections.


Background: Systemic candidiasis are serious conditions responsible for high mortality. The objective of this work is to describe the epidemiology of systemic candidiasis in high-risk departments at the UHC and the ACC of BATNA. Patients and methods: This is a descriptive prospective study over a period of three years (January 1, 2016 to December 31, 2018). The patients included are those with at least one positive deep sample for Candida spp. Results: 69 cases of systemic candidiasis corresponding to 75 isolates and concerning 63 patients could be analyzed. The overall incidence was 2.62 cases per 1,000 admissions. The main reasons for hospitalization were hematologic malignancies and septic shock. The presence of colonization ( 2 sites), broad-spectrum antibiotic therapy, an intravascular catheter, corticosteroid therapy, chemotherapy, neutropenia were the most common risk factors. Analysis of the isolated strains showed the predominance of nonalbicans species. Colonization index ≥ 0.5 was significantly associated with the risk of systemic candidiasis. Azole's use was associated with the highest mortality rate (19%). The mortality rate is significantly high 51%. Conclusion. Risk factors and a colonization index ≥ 0.5 in high-risk wards are a predictor of systemic candidiasis. Therapeutic care must be instituted to reduce the mortality rate and avoid complications linked to these infections


Subject(s)
Humans , Male , Female , Candida albicans , Disease Management , Invasive Fungal Infections , Azoles , Risk Factors , Academic Medical Centers
10.
São Paulo; s.n; 2021. 85 p. ilus.
Thesis in Portuguese | SES-SP, LILACS, SES-SP, CONASS, SESSP-CTDPROD, SES-SP, SESSP-TESESESSP, SES-SP | ID: biblio-1358927

ABSTRACT

O objetivo deste estudo foi avaliar a atividade antifúngica dos óleos essenciais de Cinnamomum cassia (L.) J.Presl, Myristica fragrans Houtt, Schinus terebinthifolius Raddi, Zingiber officinale Roscoe bem como o composto químico majoritário do óleo essencial com a menor concentração fungicida mínima sobre 16 isolados biológicos de Candida albicans. Determinar as concentrações fungicidas mínimas, o efeito dos óleos e do composto químico majoritário, em doses subinibitórias sobre a formação de tubo germinativo, clamidoconídeo e a produção de franjas, nos isolados de C. albicans, antes e após contato com os diferentes óleos essenciais e composto químico majoritário. Os quatro óleos essenciais apresentaram atividade inibitória sobre C. albicans. C. cassia (L.) J.Presl CFM 50 3,23 µg/mL e CFM 90 51,68 µg/mL ,M. fragrans Houtt CFM 50 21,437 µg/mL e CFM 90 171.500 µg/mL, S. terebinthifolius Raddi CFM 50 42.600 µg/mL e CFM 90 85.200µg/mL, Z. officinale Roscoe CFM 50 171.200µg/mL e CFM 90 resistente até a concentração de 171.200 µg/mL, sendo o óleo essencial de C. cassia (L.) J.Presl o que apresentou a menor concentração fungicida sobre os isolados de C. albicans. Nas doses subinibitórias os óleos essenciais de C. cassia (L.) J.Presl (canela), M. fragans Houtt (noz moscada), S. terebinthifolius Raddi (pimenta rosa), Z. officinale Roscoe (gengibre) não inibiram em todos os isolados a formação de tubo germinativo e clamidoconídeo, mas alteraram a produção de franjas. O composto químico majoritário aldeído cinâmico do óleo essencial de C. cassia (L.) J.Presl com CFM 50 26,64µg/mL e CFM 90 213,18 µg/mL, apresentou atividade fungicida sobre os isolados de C. albicans, mas pouca atividade inibitória na formação de tubo germinativo, clamidoconídeo e produção de franja. O óleo essencial de C. cassia (L.) J.Presl (canela), apresentou as melhores concentrações fungicidas mínimas e inibição da formação de tubo germinativo em relação ao composto químico majoritário aldeído cinâmico.


Subject(s)
Candida albicans , Oils, Volatile , Cinnamomum zeylanicum
11.
Article in Chinese | WPRIM | ID: wpr-888117

ABSTRACT

This study explored the mechanism of Sanhuang Decoction(SHD) in treating dextran sulfate sodium(DSS)-induced ulcerative colitis(UC) in mice with Candida albicans(Ca) colonization via high-throughput transcriptome sequencing. Specifically, the animal model was established by oral administration of 3.0% DSS for 7 days followed by intragastrical administration of Ca suspension at 1.0 × 10~8 cells for 4 days and then the mice were treated with SHD enema for 7 days. Afterwards, the general signs were observed and the disease activity index(DAI) was recorded every day. After mice were sacrificed, colon length and colon mucosa damage index(CMDI) were determined and the histomorphology was observed with the HE staining method. The fungal loads of feces were detected with the plate method. Anti-saccharomyces cerevisiae antibody(ASCA) and β-1,3-glucan in serum, and TNF-α, IL-1β, and IL-6 in serum and colon were detected by ELISA. High-throughput RNA sequencing method was adopted to identify transcriptome of colon tissues from the control, model and SHD(15.0 g·kg~(-1)) groups. Differentially expressed genes(DEGs) among groups were screened and the GO and KEGG pathway enrichment analysis of the DEGs was performed. The expression levels of NLRP3, ASC, caspase-1, and IL-1β genes related to the NOD-like receptor signaling pathway which involved 9 DEGs, were examined by qRT-PCR and Western blot. The results demonstrated that SHD improved the general signs, decreased DAI and Ca loads of feaces, alleviated colon edema, erosion, and shortening, and lowered the content of β-1,3-glucan in serum and TNF-α, IL-1β, and IL-6 in serum and colon tissues of mice. Transcriptome sequencing revealed 383 DEGs between SHD and model groups, which were mainly involved in the biological processes of immune system, response to bacterium, and innate immune response. They were mainly enriched in the NOD-like signaling pathway, cytokine-cytokine interaction pathway, and retinol metabolism pathway. Moreover, SHD down-regulated the mRNA and protein levels of NLRP3, caspase-1, and IL-1β. In a word, SHD ameliorates DSS-induced UC in mice colonized with Ca, which probably relates to its regulation of NOD-like receptor signaling pathway.


Subject(s)
Animals , Candida albicans/genetics , Colitis, Ulcerative/genetics , Colon , Dextran Sulfate/toxicity , Disease Models, Animal , Drugs, Chinese Herbal , High-Throughput Nucleotide Sequencing , Mice , Transcriptome
12.
Article in Chinese | WPRIM | ID: wpr-878924

ABSTRACT

The aim of this paper was to investigate the effect of berberine hydrochloride on the cell wall integrity of Candida albicans hypha. The minimal inhibitory concentration(MIC) of berberine hydrochloride against clinical and standard C. albicans strains was detected by micro liquid-based dilution method; the effect of berberine hydrochloride on the colony formation of C. albicans SC5314 was investigated by spot assay; the effect of berberine hydrochloride on the metabolism of C. albicans SC5314 hypha was checked by XTT reduction assay, and the viability of C. albicans SC5314 hypha was tested by fluorescent staining assay. The effect of berberine hydrochloride on the morphology of C. albicans SC5314 hypha was examined by scanning electron microscope. The changes in the cell wall of C. albicans SC5314 hypha after berberine hydrochloride treatment were detected by transmission electron microscopy. The effect of berberine hydrochloride on β-glucan from C. albicans SC5314 was detected by flow cytometry. The effect of berberine hydrochloride on hypha-specific gene ECE1 and β-glucan synthase genes FKS1 and FKS2 in C. albicans was examined by qRT-PCR. The results showed that berberine hydrochloride showed a strong inhibitory effect on both clinical and standard strains of C. albicans, and the MIC was 64-128 μg·mL~(-1). Spot assay, XTT redunction assay and fluorescent staining assay showed that with the increase of berberine hydrochloride concentration, the viability of C. albicans SC5314 gradually decreased. The transmission electron microscopy scanning assay showed that this compound could cause cell wall damage of C. albicans. The flow cytometry analysis showed the exposure degree of C. albicans β-glucan. The qRT-PCR further showed that berberine hydrochloride could significantly down-regulate hypha-specific gene ECE1 and β-glucan synthase-related gene FKS1 and FKS2. In conclusion, this compound can down-regulate C. albicans and β-glucan synthase-related gene expressions, so as to destroy the cell wall structure of C. albicans, expose β-glucan and damage the integrity of the wall.


Subject(s)
Antifungal Agents/pharmacology , Berberine/pharmacology , Candida albicans/genetics , Cell Wall , Hyphae , Microbial Sensitivity Tests
13.
Rev. biol. trop ; 69(1): 36-44, 2021. tab, graf
Article in Spanish | MTYCI, LILACS, MTYCI | ID: biblio-1290965

ABSTRACT

Introduction: In recent decades, studies related to the search and characterization of bioactive molecules in marine organisms have increased exponentially, demonstrating the enormous wealth of secondary metabolites of diverse structural composition that cannot be found in organisms present in the terrestrial environment. A significant number of the new marine natural compounds discovered have contributed to solving some of the problems of humanity, mainly those related to human health. Objective: The purpose of this research is to evaluate the bactericidal and fungicidal activities of the methanolic extract of sea cucumber Holothuria princeps collect from the bay of Cispatá in the Colombian Caribbean, in addition to chemically identifying its fatty acids. Methods: A methanolic extraction was performed from the collected biological material, by the cold maceration method. The extract obtained was fractionated using chromatographic techniques and the fatty acids were obtained, which were derivatized and identified by means of gas chromatography in coupling with mass spectrometry. The antibacterial and antifungal activities of the methanolic extract of Holothuria princeps was performed through the microdilution method against reference strains and clinical isolates. Results: We found 16 fatty acids present in Holothuria princeps according to the analysis of their mass spectra. Antibacterial activity showed that Enterococcus faecalis was the most susceptible to the extract at low concentrations, while Pseudomonas aeruginosa was the highest at the higher concentrations. In antifungal treatment, the fungus with the highest inhibition was the clinical isolate of Candida albicans (blood sample). Conclusions: Taking into account previous studies in the genus Holothuria, it is considered that the environment plays a fundamental role in the presence and diversity of fatty acids. The evaluation of the antibacterial activity against reference strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae and Enterococcus faecalis demonstrated the existence of a considerable effect in the reduction of bacterial growth by the extracts applied, mainly at low concentrations (less than 1 000 ppm). On the other hand, the antifungal activity against the reference strain of Candida albicans and the clinical isolates of Candida albicans (blood sample) and Candida krusei (catheter sample), the extract showed that the best results were presented at higher concentrations (above 1 500 ppm).


Introducción: En las últimas décadas los estudios relacionados con la búsqueda y caracterización de moléculas bioactivas en organismos marinos han aumentado de una manera exponencial, lo que demuestra la enorme riqueza de metabolitos secundarios de diversa composición estructural que no pueden ser encontrados en organismos presentes en el medio terrestre. Estas nuevas moléculas halladas poseen numerosas actividades biológicas que ayudan a resolver muchos problemas que ha tenido el hombre a lo largo de su existencia, lo que las convierte en productos de gran importancia para la humanidad. Objetivo: El propósito de este estudio es la identificación de los ácidos grasos presentes en el pepino de mar Holothuria princeps recolectado en costas del Caribe colombiano, además del análisis de las actividades antibacterianas y antifúngicas de su extracto metanólico frente a cepas de referencia y aislados clínicos. Métodos: Del material biológico recolectado se realizó una extracción metanólica usando el método de maceración en frío. El extracto obtenido se fraccionó usando cromatografía en columna y se lograron obtener los ácidos grasos, los cuales fueron derivatizados e identificados por medio de cromatografía de gases en acople con espectrometría de masas. La actividad antibacteriana y antifúngica del extracto metanólico de Holothuria princeps se realizó a través del método de microdilución. Resultados: Los resultados arrojaron la identificación de 16 ácidos grasos presentes en Holothuria princeps de acuerdo con el análisis de sus espectros de masas. La actividad antibacteriana mostró que Enterococcus faecalis fue la bacteria más susceptible al efecto del extracto a bajas concentraciones, mientras que a las más altas lo fue Pseudomonas aeruginosa. A nivel general en el tratamiento antifúngico, el hongo que presentó una mayor inhibición fue el aislado clínico de Candida albicans (muestra de sangre). Conclusiones: Teniendo en cuenta estudios previos en organismos del mismo género, se puede considerar en cuanto a los ácidos grasos identificados, que el entorno juega un papel fundamental en la presencia y composición de estos compuestos. La evaluación de la actividad antibacteriana contra cepas de referencia de Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae y Enterococcus faecalis, demostró la existencia de un efecto considerable en la reducción del crecimiento bacteriano por parte de los extractos utilizados, principalmente a bajas concentraciones (menos de 1 000 ppm). En cuanto


Subject(s)
Holothuria , Anti-Bacterial Agents , Antifungal Agents , Mass Spectrometry , Candida albicans , Chromatography
14.
São José dos Campos; s.n; 2021. 59 p. ilus, graf, tab.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1359953

ABSTRACT

Candida albicans possui capacidade de causar uma ampla variedade de manifestações clínicas devido a múltiplos fatores de virulência que agem simultaneamente para vencer o sistema imune e invadir os tecidos do hospedeiro. Estudos recentes demonstraram que o crescimento de C. albicans pode ser inibido por metabólitos produzidos por Streptococcus mutans, que estão presentes no sobrenadante da cultura bacteriana. Assim, o objetivo desse estudo foi investigar se o sobrenadante da cultura de S. mutans, além de inibir o crescimento de C. albicans, é capaz de atenuar os mecanismos de virulência desse fungo. Inicialmente, uma suspensão padronizada de S. mutans (107 células/mL) foi incubada em caldo BHI a 37ºC por 4 h em 5% de CO2. O crescimento em BHI foi filtrado em membrana de 0,22 µm, obtendo-se o sobrenadante da cultura de S. mutans livre de células. A seguir, uma suspensão padronizada de C. albicans (107 células/mL) foi adicionada ao sobrenadante filtrado da cultura de S. mutans em caldo BHI, sendo incubada a 37ºC por 24 h. Para os grupos controle, a suspensão de C. albicans foi colocada em caldo BHI ou YPD esterilizados e incubada nas mesmas condições. Após o período de incubação, o crescimento de C. albicans foi centrifugado e lavado para obtenção de uma suspensão padronizada de C. albicans contendo as células sobreviventes da exposição ao sobrenadante de S. mutans. Essa suspensão de C. albicans foi então utilizada nos testes in vitro e in vivo para determinação dos fatores de virulência desse micro-organismo. No estudo in vitro, foi investigada a atividade proteolítica extracelular de C. albicans, bem como sua capacidade de filamentação, adesão e formação de biofilmes (1:30, 6, 24 e 48 h). Para o estudo in vivo, foi utilizado o modelo de Galleria mellonella, analisando-se a curva de sobrevivência, o número de células fúngicas e hemócitos na hemolinfa de larvas infectadas por C. albicans. Para a análise estatística foi utilizado ANOVA, teste de Tukey, Kruskal-Wallis e Log-rank, com nível de significância de 5%. Verificou-se que as células de C. albicans expostas ao sobrenadante de S. mutans apresentaram redução na filamentação, formação de biofilmes e patogenicidade em G. mellonella em relação ao controle. A exposição ao sobrenadante de S. mutans também mudou o padrão de aderência de C. albicans. Entretanto, o sobrenadante de S. mutans não reduziu a atividade proteolítica de C. albicans. Concluiu-se que o sobrenadante de S. mutans apresentou capacidade de inibir importantes mecanismos de virulência de C. albicans, podendo ser uma fonte de novos agentes antifúngicos a ser explorada


Candida albicans has the ability to cause a wide variety of clinical manifestations due to multiple virulence factors that act simultaneously to overcome the immune system and invade host tissues. Recent studies have shown that the growth of C. albicans can be inhibited by metabolites produced by Streptococcus mutans. Thus, the objective of this study was to investigate whether the culture supernatant of S. mutans is able to attenuate the virulence mechanisms of this fungus. Initially, a standardized suspension of S. mutans (107 cells/mL) was incubated in BHI broth at 37ºC for 4 h in 5% CO2. The growth in BHI was filtered through a 0.22 µm membrane, obtaining the cell free culture supernatant of S. mutans. Then, a standardized suspension of C. albicans (107 cells/mL) was prepared and added to the supernatant of the culture of S. mutans in BHI broth, being incubated at 37ºC for 24 h. For the control groups, the suspension of C. albicans was placed in sterile BHI or YPD broth and incubated under the same conditions. After the incubation period, the growth of C. albicans was centrifuged and washed to obtain a standardized suspension of C. albicans containing the surviving cells from exposure to the S. mutans supernatant. This suspension of C. albicans was then used to perform in vitro and in vivo tests to determine the virulence factors of this microorganism. In the in vitro study, the extracellular proteolytic activity of C. albicans was investigated, as well as its capacity for filamentation, adhesion and biofilm formation (1:30, 6, 24 and 48 h). For the in vivo study, the Galleria mellonella model was used, analyzing the survival curve, the number of fungal cells and hemocytes in the hemolymph of larvae infected with C. albicans. For statistical analysis, ANOVA, Tukey's test, Kruskal-Wallis and Log-rank were used, with a significance level of 5%. It was found that the cells of C. albicans exposed to the supernatant of S. mutans showed a reduction in filament, formation of biofilms and pathogenicity in G. mellonella in relation to the control. Exposure to the S. mutans supernatant also changed the pattern of adherence of C. albicans. However, the S. mutans supernatant did not reduce the proteolytic activity of C. albicans. It was concluded that the supernatant of S. mutans had the capacity to inhibit important mechanisms of virulence of C. albicans, being able to be a source of new antifungal agents to be explored.


Subject(s)
Animals , Streptococcus mutans , Candida albicans , Virulence Factors , Virulence , Analysis of Variance , Statistics, Nonparametric , Biofilms
15.
São José dos Campos; s.n; 2021. 54 p. ilus, graf.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1362071

ABSTRACT

A terapia fotodinâmica (TFD) vem se mostrando como um método eficaz no controle de micro-organismos patogênicos, sendo investigada para o tratamento de diversas doenças infecciosas, como a candidose bucal. Recentemente, alguns agentes potencializadores dessa terapia têm sido estudados, incluindo a Quitosana (QT), um polímero natural extraído do exoesqueleto de crustáceos. O objetivo desse estudo foi avaliar o efeito potencializador da QT na TFD mediada pelo fotossensibilizador Azul de Metileno (AM) sobre cepas de Candida albicans, investigando sua ação em culturas planctônicas, biofilmes e células persistentes ao fluconazol. Além disso, foi avaliado a capacidade da QT em interferir na absorção do AM pelas células de Candida. Foram utilizadas duas cepas de C. albicans, sendo uma padrão (ATCC 18804) e uma clínica isolada de candidose orofaríngea (70). Para os ensaios em culturas planctônicas, as cepas de C. albicans foram cultivadas em caldo Yeast Nitrogen Base (YNB) por 24 horas. Os biofilmes foram formados por 48 horas no fundo das placas de 96 poços. Para indução de células persistentes, C. albicans foi cultivada com altas concentrações de fluconazol por 48 horas. A seguir, foram realizados os tratamentos com QT a 5 mg/mL (pH de 6,5), AM nas concentrações de 300 ou 600 µM, e irradiação com LED (660 nm) na densidade de energia de 30 J/cm2. Foram incluídos oito grupos experimentais: TFD com AM e QT na presença de Luz (AM+QT+L+), AM e QT sem Luz (AM+QT+L-), QT e Luz (QT+L+), QT sem Luz (QT+L-), TFD com AM e Luz (AM+L+), AM sem Luz (AM+L-), Solução Fisiológica com Luz (F-L+) e apenas Solução Fisiológica (F-L-). Após os tratamentos, foi realizada contagem de unidades formadoras de colônias (UFC/mL). Para determinar a absorção do fotossensibilizador pelas células de C. albicans, as células foram lisadas e centrifugadas para leitura da densidade óptica. Os dados foram analisados por ANOVA e teste de Tukey (p<0,05%). Na TFD em culturas planctônicas, o AM (300 µm) reduziu as células de Candida em 1,6 log (UFC/mL), enquanto que a associação AM+QT levou à redução de 4,7 log. Na TFD em biofilmes, ocorreu redução microbiana de 2,9 log para o tratamento com AM (600 µm) e de 5,3 log para AM+QT. Em relação às células persistentes, a redução encontrada foi de 0,8 log para AM e 1,5 log para AM+QT. No teste de absorção, a penetração do AM nas células de Candida (DO 0,02) foi aumentada na presença da QT (DO 0,39). Concluiu-se que a QT potencializou o efeito antimicrobiano da TFD em culturas planctônicas, biofilmes e células persistentes de C. albicans, provavelmente por facilitar a penetração do AM nas células fúngicas.


Photodynamic therapy (PDT) has been shown to be an effective method to control pathogenic microorganisms, being investigated for the treatment of several infectious diseases, such as oral candidiasis. Recently, some agents that enhance this therapy have been studied, including Chitosan (CS), a natural polymer extracted from the exoskeleton of crustaceans. The aim of this study was to evaluate the potentiating effect of CS on Methylene Blue (MB) photosensitizer-mediated PDT on Candida albicans strains, investigating its action on planktonic cultures, biofilms and persister cells to fluconazole. In addition, the ability of CS to interfere with MB absorption by Candida cells was evaluated. Two strains of C. albicans were used, one standard (ATCC 18804) and one isolated clinical of oropharyngeal candidosis (70). For assays in planktonic cultures, C. albicans strains were cultivated in Yeast Nitrogen Base broth (YNB) for 24 hours. Biofilms were formed for 48 hours at the bottom of 96-well plates. For the induction of persister cells, C. albicans was cultivated with high concentrations of fluconazole for 48 hours. Next, treatments were performed with CS at 5 mg/mL (pH 6.5), MB at concentrations of 300 or 600 µM, and irradiation with LED (660 nm) at an energy density of 30 J/cm2. eight experimental groups were included: PDT with MB and CS in the presence of Light (MB+CS+L+), MB and CS without Light (MB+CS+L-), CS and Light (CS+L+), CS without Light (CS +L-), PDT with MB and Light (MB+L+), MB without Light (MB+L-), Physiological Solution with Light (F-L+) and Physiological Solution only (FL-). After the treatments, colony forming units (CFU/mL) were counted. To determine the absorption of the photosensitizer by C. albicans cells, the cells were lysed and centrifuged for optical density reading. Data were analyzed by ANOVA and Tukey test (p<0.05%). In PDT in planktonic cultures, MB (300 µm) reduced Candida cells by 1.6 log (CFU/mL), while the MB+CS association led to a 4.7 log reduction. In PDT in biofilms, there was a microbial reduction of 2.9 log for the treatment with MB (600 µm) and of 5.3 log for MB+CS. Regarding persister cells, the reduction found was 0.8 log for MB and 1.5 log for MB+CS. In the absorption test, the penetration of MB into Candida cells (OD 0.02) was increased in the presence of CS (OD 0.39). It was concluded that CS potentiated the antimicrobial effect of PDT in planktonic cultures, biofilms and persister cells of C. albicans, probably by facilitating the penetration of MB into fungal cells .


Subject(s)
Photochemotherapy , Candida albicans , Chitosan , Methylene Blue , Analysis of Variance , Photosensitizing Agents , Biofilms
17.
J. appl. oral sci ; 29: e20210024, 2021. tab, graf
Article in English | LILACS | ID: biblio-1340097

ABSTRACT

Abstract Understanding the behavior of Candida spp. when exposed to denture disinfectants is essential to optimize their effectiveness. Changes in the virulence factors may cause increased resistance of Candida spp. to disinfectant agents. Objective To evaluate the microbial load, cellular metabolism, hydrolytic enzyme production, hyphae formation, live cell and biofilm quantification of Candida albicans, Candida tropicalis and Candida glabrata after exposure to disinfectant solutions. Methodology Simple biofilms were grown on heat-polymerized acrylic resin specimens, and divided into groups according to solutions/strains: distilled water (control); 0.25% sodium hypochlorite (NaOCl 0.25% ); 10% Ricinus communis (RC 10%); and 0.5% Chloramine T (CT 0.5%). The virulence factors were evaluated using the CFU count (microbial load), XTT method (cell metabolism), epifluorescence microscopy (biofilm removal and live or dead cells adhered), protease and phospholipase production and hyphae formation. Data were analyzed (α=0.05) by one-way ANOVA/ Tukey post hoc test, Kruskal-Wallis test and Wilcoxon test. Results NaOCl 0.25% was the most effective solution. CT 0.5% reduced the number of CFUs more than RC 10% and the control. RC 10% was effective only against C. glabrata. RC 10% and CT 0.5% decreased the cellular metabolism of C. albicans and C. glabrata. Enzyme production was not affected. Hyphal growth in the RC 10% and CT 0.5% groups was similar to that of the control. CT 0.5% was better than RC 10% against C. albicans and C. tropicalis when measuring the total amount of biofilm and number of living cells. For C. glabrata, CT 0.5% was equal to RC 10% in the maintenance of living cells; RC 10% was superior for biofilm removal. Conclusions The CT 0.5% achieved better results than those of Ricinus communis at 10%, favoring the creation of specific products for dentures. Adjustments in the formulations of RC 10% are necessary due to efficacy against C. glabrata. The NaOCl 0.25% is the most effective and could be suitable for use as a positive control.


Subject(s)
Candida , Disinfectants , Acrylic Resins , Candida albicans , Biofilms , Virulence Factors
18.
J. appl. oral sci ; 29: e20200865, 2021. graf
Article in English | LILACS | ID: biblio-1286916

ABSTRACT

Abstract Denture stomatitis is the most frequent oral lesion in removable prosthesis wearers, with high recurrence rates and a complex treatment. Objective This study describes a protocol to obtain and to contaminate a palatal device with Candida albicans biofilm that could be used for an animal model of denture stomatitis. Methodology Acrylic resin devices (N=41) were obtained from impressions of the palates of Wistar rats with individual trays and polyether. The efficacy of microwave irradiation (MW), ultraviolet light (UV), or ultrasonic bath (US) was assessed by colony viability and spectrophotometric analyses (n=5) in order to select the most appropriate method for sterilizing the devices. Then, different devices (n=5) were contaminated with C. albicans and evaluated by CFU/mL determination, scanning electron microscopy, and laser confocal microscopy. Device stabilization was assessed with either autopolymerizing acrylic resins or a self-adhesive resin cement (n=2). The spectrophotometric data were analyzed by one-way ANOVA followed by the Tukey's HSD post-hoc test (α=0.05). Results MW was the only method capable of sterilizing the devices, and the contamination protocol developed a mature and viable C. albicans biofilm (~1.2 x 106 CFU/mL). The self-adhesive resin cement was the best stabilization material. Conclusions This acrylic resin palatal device was designed to be similar to the clinical situation of contaminated prostheses, with easy manufacturing and handling, effective stabilization, and satisfactory contamination. Thus, the acrylic device can be a valuable tool in the development of denture stomatitis in rats.


Subject(s)
Animals , Rats , Stomatitis, Denture , Candida albicans , Palate , Acrylic Resins , Rats, Wistar , Biofilms , Denture Bases
19.
Arq. odontol ; 57: 141-148, jan.-dez. 2021. ilus, tab
Article in English | LILACS, BBO | ID: biblio-1343550

ABSTRACT

Aim: To evaluate the effect of three natural antifungal agents combined with routine denture care on the treatment of DS, using a quantitative mycological culture analysis. Methods: Thirty denture wearers with denture stomatitis DS were treated using five substances: sterile distilled water (G1), nystatin oral suspension (G2), 20% alcoholic extract propolis (G3), Punica granatumLinné gel (G4), and Uncaria tomentosa gel (G5). The substances were used 3 times a day for 14 days. Quantitative mycological culture analysis of samples collected from the palatal mucosa was performed at three stages: before treatment (T0), after 14 days of treatment (T1), and 30 days after treatment completion (T2). Data were evaluated using Kruskal-Wallis and Friedman tests (p < 0.05). Results: Palatal mucosa intragroup analysis showed a significant reduction of Candida CFU/mL values for all groups at T1 compared to T0 (p < 0.05). However, they did not present statistical differences when comparing T1 and T2 (p > 0.05). The intergroup analysis demonstrated that there are no statistical differences, regardless of the evaluation time (p > 0.05). Conclusion:The natural products tested showed a satisfactory result on DS treatment, which proved to be equivalent to conventional topical therapy with nystatin and to treatment using only regular oral hygiene procedures.


Objetivo: Avaliar o efeito de três antifúngicos naturais combinados com o cuidado rotineiro com próteses dentárias no tratamento da EP, por meio de uma análise quantitativa de cultura micológica. Métodos: Trinta usuários de próteses dentárias com EP foram tratados com cinco substâncias: água destilada estéril (G1), suspensão oral de nistatina (G2), extrato alcoólico de própolis 20% (G3), gel Punica granatum L. (G4) e gel Uncaria tomentosa (G5). As substâncias foram utilizadas 3 vezes ao dia durante 14 dias. A análise micológica quantitativa das amostras coletadas da mucosa palatina foi realizada em três etapas: antes do tratamento (T0), após 14 dias do tratamento (T1) e 30 dias após o término do tratamento (T2). Os dados foram avaliados pelos testes de Kruskal-Wallis e Friedman (p < 0,05). Resultados: A análise intragrupo da mucosa palatina mostrou uma redução significativa dos valores de Candida UFC/mL para todos os grupos em T1 em comparação com T0 (p < 0,05). No entanto, não apresentaram diferenças estatísticas na comparação de T1 e T2 (p > 0,05). A análise intergrupos demonstrou que não há diferenças estatísticas, independentemente do tempo de avaliação (p > 0,05). Conclusão: Os produtos naturais testados apresentaram resultado satisfatório no tratamento da EP, sendo equivalente à terapia tópica convencional com nistatina e ao tratamento apenas com procedimentos rotineiros de higiene bucal.


Subject(s)
Stomatitis, Denture , Biological Products , Candida albicans , Colony Count, Microbial , Antifungal Agents , Propolis , Distilled Water , Nystatin
20.
Article in English | LILACS, BBO | ID: biblio-1250450

ABSTRACT

ABSTRACT Objective: To investigate the antimicrobial activity of colloidal selenium nanoparticles in chitosan solution (Cts-Se-NPs) against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. Material and Methods: Cts-Se-NPs solution was prepared using a simple chemical reduction method. The MIC and MBC against S. mutans, L. acidophilus, and C. albicans were determined using the broth dilution assay. Results: The Cts-Se-NPs had remarkable antimicrobial activity against S. mutans, L. acidophilus, and C. albicans. The MIC values of the Cts-Se-NPs were lowest for S. mutans (0.068 mg/ml) compared to L. acidophilus (0.137 mg/ml), and C. albicans (0.274 mg/ml). The MBC values of the Cts-Se-NPs against the microorganisms after one, two, six, and 24 hours indicated that the concentration of 0.274 mg/ml of Cts-Se-NPs completely killed S. mutans, L. acidophilus, and C. albicans after one, two, and six hours, respectively. At the concentration of 0.137 mg/ml, S. mutans and L. acidophilus were killed after six and 24 hours, respectively. Conclusion: These findings encourage the potential use of Cts-Se-NPs in dentistry, while further clinical research is required in this area.


Subject(s)
Selenium , Streptococcus mutans , Dentistry , Chitosan , Nanoparticles , Candida albicans , Iran , Lactobacillus acidophilus
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