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1.
An. bras. dermatol ; 97(1): 93-95, Jan.-Feb. 2022. graf
Article in English | LILACS | ID: biblio-1360086

ABSTRACT

Abstract Jorge Lobo's disease (JLD) is a chronic, granulomatous fungal infection caused by the traumatic implantation of the fungus Lacazia loboi in the cutaneous and subcutaneous tissues, with the presence of isolated nodular and coalescent keloidal lesions. Malignant degeneration is rare. This case report describes a 64-year-old male patient with JLD for 30-years who showed a change in the aspect of a lesion in the left lower limb. Histopathological examination confirmed the progression to well-differentiated squamous cell carcinoma (SSC). JLD is highly prevalent in tropical and subtropical regions, requiring monitoring concerning the transformation into SSC in long-term lesions.


Subject(s)
Humans , Male , Carcinoma, Squamous Cell , Lacazia , Lobomycosis , Skin , Middle Aged
2.
Rev. Ciênc. Plur ; 8(1): e24820, 2022. tab, graf
Article in Portuguese | LILACS, BBO | ID: biblio-1348511

ABSTRACT

Introdução:O carcinoma de células escamosas de cavidade oral e orofaringe é uma neoplasia epitelial maligna comum, respondendo pela maioria dos casos de tumores de cabeça e pescoço. Ele está relacionado a hábitos comportamentais, como tabagismo e etilismo de longa duração, e à infecção pelo Papilomavírus humano. Objetivos:Esse estudo objetivou descrever o perfil epidemiológico dos pacientes diagnosticados com essa neoplasia na Liga Mossoroense de Estudos e Combate ao Câncer. Metodologia: Foi realizado um estudo observacional com delineamento transversal a partir de dados presentes nos prontuários clínicos e laudos anatomopatológicos e no Sistema de Informações sobre Mortalidade no período entre 2006 a 2018. Os dados foram analisados a partir do Software R, utilizandoo teste de Wilcoxon-Mann-Whitney para as análises inferenciais e o método de Kaplan-Meier para análise da sobrevida. Resultados:225 prontuários foram analisados, sendo 70,22% de homens, 65,33% na faixa etária entre 46-70 anos e cor branca (51,57%). Destes, 25,78% eram tabagistas e 39,11% tabagistas e etilistas. O principal tratamento identificado foi a associação de cirurgia, quimioterapia e radioterapia. Observou-se que 49,10% dos óbitos foram em decorrência dessa neoplasia. O principal estádio patológico encontrado foi o quatro A (34,22%). Foi identificada maior sobrevida nos pacientes acima de 70 anos, cujo tratamento foi exclusivamente cirúrgico. Menor sobrevida foi identificada em indivíduos que tinham associação de hábitos (etilismo e tabagismo). Conclusões:Nossos resultados sugerem que a evolução à óbito foi o principal desfecho clínico e, isso pode estar relacionado aos hábitos comportamentais que influenciam diretamente o curso e prognóstico da doença. Ademais, destaca-se a importância do diagnóstico precoce a fim de reduzir óbitos e melhorar a qualidade de vida dos indivíduos, assim como a necessidade de implementar políticas educativas sobre os principais fatores de risco associados ao desenvolvimento dessa neoplasia (AU).


Introduction: Squamous cell carcinoma of the oral cavity and oropharynx is a common malignant epithelial neoplasm, accounting for most cases of head and neck tumors. It is related to behavioral habits, such as long-standing smoking and alcoholism, as well as to the human Papillomavirus infection. Objectives: This study aimed at describing the epidemiological profile of the patients diagnosed with this neoplasm in the Mossoró League for Studying and Combating Cancer. Methodology:An observational study with a cross-sectional design was carried out based on data present in the medical records and anatomopathological reports and in the Mortality Information System during the 2006-2018 period. The data were analyzed using the R Software, resorting to the Wilcoxon-Mann-Whitneytest for the inferential analyses and to the Kaplan-Meier method for survival analysis. Results: 225 medical records were analyzed: 70.22% belonging to men, 65.33% aged between 46 and 70 years old and white-skinned (51.57%). Of these, 25.78% were smokers and 39.11% were smokers and alcoholics. The main treatment identified was the association of surgery, chemotherapy and radiotherapy. It was observed that 49.10% of the deaths were due to this neoplasm. The main pathological stage found wasfour A(34.22%).Longer survival was identified in patients over 70 years of age, whose treatment was exclusively surgical. Shorter survival was identified in individuals who had associated habits (alcoholism and smoking). Conclusions:Our results suggest that evolution to death was the main clinical outcome; this can be related to the behavioral habits that exert a direct influence on the course and prognosis of the disease. Furthermore, the importance of early diagnosis is highlighted in order to reduce the number of deaths and improve the individuals' quality of life, as well as the need to implement educational policies on the main risk factors associated with the development of this neoplasm (AU).


Introducción: El carcinoma de células escamosas de la cavidad oral y la orofaringe es una neoplasia epitelial maligna común, que representa la mayoría de los casos de tumores de cabeza y cuello. Se relaciona con hábitos de comportamiento, como el tabaquismo y el alcoholismo, y la infección por el virus papiloma humano. Objetivos:Este estudio tuvo como objetivo describir el perfil epidemiológico de los pacientes diagnosticados con esta neoplasiaen la Liga Mossoroense de Estudios y Combate al Cáncer. Metodología: Se realizó un estudio observacional, transversal a partir de los datos presentes en las historias clínicas e informes patológicos y en el Sistema de Información de Mortalidad en el período 2006-2018. Los datos se analizaron mediante el Software R, con utilización de la Prueba de Wilcoxon-Mann-Whitney para análisis inferencial y el método de Kaplan-Meier para análisis de supervivencia. Resultados:Se analizaron 225 historias clínicas, 70,22% en hombres, 65,33% con edades entre 46-70 años y blancos (51,57%). De estos, 25,78% eran fumadores y 39,11% eran fumadores y alcohólicos. El principal tratamiento identificado fue la asociación de cirugía, quimioterapia y radioterapia. 49,10% de las muertes se debieron a esta neoplasia. El principal estadio patológico encontrado fue cuatro A (34,22%). Se identificó mayor sobrevida en pacientes mayores de 70 años, cuyo tratamiento fue exclusivamente quirúrgico. Se identificó una menor sobrevida en personas que tenían hábitos asociados. Conclusiones:Nuestros resultados sugieren que la evolución hacia la muerte fue el principal resultado clínico y esto puede estar relacionado con hábitos de comportamiento que influyen directamente en el curso y pronóstico de la enfermedad. Además, se destaca la importancia del diagnóstico precoz para reducir las muertes y mejorar la calidad de vida, así como la necesidad de implementar políticas educativas sobre los principales factores de riesgo asociados al desarrollo de esta neoplasia (AU).


Subject(s)
Humans , Health Profile , Brazil/epidemiology , Mouth Neoplasms/pathology , Carcinoma, Squamous Cell/pathology , Oropharyngeal Neoplasms/pathology , Head and Neck Neoplasms/pathology , Tobacco Use Disorder , Survival Analysis , Epidemiology , Cross-Sectional Studies , Statistics, Nonparametric , Papillomavirus Infections , Research Report , Smokers
3.
São Paulo; s.n; 2022. 55 p. tab, ilus.
Thesis in Portuguese | LILACS, Inca | ID: biblio-1362687

ABSTRACT

Introdução: O carcinoma escamoso do pênis é uma neoplasia rara, mas sua incidência e mortalidade específica vêm aumentando. No Brasil, o câncer de pênis constitui 2,1% das neoplasias em homens. O acometimento dos linfonodos inguinais é o fator prognóstico mais importante. Comparados aos pacientes com linfonodos positivos, aqueles com linfonodos histologicamente negativos (pN0) têm o melhor prognóstico; entretanto, a ausência de metástases linfonodais não garante a cura, haja vista que uma proporção destes apresenta recorrência locorregional ou progressão tumoral após o tratamento. Poucos estudos examinaram populações de pacientes com pN0 ou clinicamente negativos (cN0). Objetivos: Avaliar o impacto da expressão imuno-histoquímica da E-caderina e da vimentina como fatores prognósticos para sobrevida global, sobrevida câncer específica e sobrevida livre de doença em pacientes com carcinoma escamoso do pênis clinicamente ou histologicamente negativos para metástase linfonodal. Material e Métodos: Coorte retrospectiva de 116 pacientes cN0 e pN0 com carcinoma escamoso do pênis consecutivamente tratados no A.C.Camargo Cancer Center entre 1953-2017. As variáveis registradas incluíram idade, estadiamento TNM, subtipo histológico, grau de diferenciação tumoral, invasão vascular microscópica, invasão perineural, padrão microscópico da fronte de invasão tumoral (expansivo/infiltrativo) e expressão imuno-histoquímica das proteínas E-caderina e vimentina. As amostras de tumor foram analisadas no centro do tumor e na fronte de invasão tumoral. As análises de sobrevida foram realizadas usando curvas de Kaplan-Meier e testes de log-rank. O modelo de riscos proporcionais de Cox foi usado para determinar quais variáveis influenciaram as taxas de sobrevida global, câncer específica e livre de doença. Resultados: O tempo de seguimento médio foi de 176 meses. A presença de invasão perineural foi associada à alta expressão de vimentina no centro tumoral (p = 0,013), à perda da expressão de Ecaderina no centro do tumor (p = 0,026) e na fronte de invasão tumoral (p = 0,02). A perda da expressão imuno-histoquímica da E-caderina na fronte de invasão e a presença de invasão perineural foram independentemente associadas à sobrevida livre de doença. Conclusões: A perda da expressão imuno-histoquímica da E-caderina na fronte de invasão tumoral e a presença de invasão perineural no tumor primário foram associadas a uma menor sobrevida livre de doença. Alta expressão de vimentina no centro do tumor, perda da expressão da Ecaderina no centro do tumor e na fronte de invasão do tumor foram associadas à presença de invasão perineural no tumor primário.


Introduction: Penile squamous cell carcinoma is a rare neoplasm, but its incidence and specific mortality have been increasing. In Brazil, penile squamous cell carcinoma constitutes 2.1% of neoplasms in men. The involvement of inguinal lymph nodes is the most important prognostic factor. Compared to patients with positive lymph nodes, histologically negative patients (pN0) have the best prognosis; however, the absence of lymph node metastases does not guarantee a cure. Some pN0 patients exhibit locoregional recurrence or tumor progression after treatment. Few studies have examined patient populations with histologically negative (pN0) or clinically negative (cN0) lymph nodes. Objectives: To evaluate the impact of E-cadherin and vimentin expression as prognostic factors for overall survival, cancer-specific survival, and disease-free survival in patients with penile squamous cell carcinoma who were clinically or histologically negative for lymph node metastasis. Material and Methods: Retrospective cohort of 116 patients (cN0 and pN0) treated at A. C. Camargo Cancer Center from 1953 to 2017. Recorded variables included age, TNM staging, histological subtype, degree of tumor differentiation, microscopic vascular invasion, perineural invasion, microscopic pattern of the tumor invasion front (pushing/infiltrating) and vimentin and E-cadherin immunohistochemical expression. Tumor samples were analyzed at the tumor center and at the tumor invasion front. Survival analyses were performed using Kaplan-Meier curves and log-rank testing. Cox's proportional hazards model was used to determine which variables influenced overall survival, disease-free survival, or cancer specific survival. Results: The median follow-up time was 176 months. Perineural invasion was associated with high vimentin expression at tumor center (p = 0.013), loss of E-cadherin expression in the tumor center (p = 0.026) and at the front invasion (p = 0.02). Loss of immunohistochemical E-cadherin expression at the front invasion and the presence of perineural were independently associated with disease-free survival. Conclusions: Lower disease-free survival was associated with loss of immunohistochemical E-cadherin expression at the tumor invasion front, and the presence of perineural invasion. High vimentin expression in the tumor center, loss of E-cadherin expression in the tumor center and in the tumor invasion front were associated with the presence of perineural invasion in the primary tumor.


Subject(s)
Humans , Male , Adult , Middle Aged , Aged , Aged, 80 and over , Penile Neoplasms , Vimentin , Cadherins , Prognosis , Immunohistochemistry , Carcinoma, Squamous Cell , Survival Analysis , Lymph Node Excision , Neoplasm Metastasis
4.
Braz. dent. sci ; 25(2): 1-9, 2022. tab
Article in English | LILACS, BBO | ID: biblio-1363701

ABSTRACT

Objective: Tobacco smoke is composed of cancer-causing chemicals referred to as carcinogens. These carcinogens are metabolized by the enzymes of the cytochrome P450 (CYP) family. Our objective was to evaluate the correlation of tobacco consumption parameters with CYP1A1, CYP1B1 and CYP2A6 expression using qRT-PCR in samples of oral squamous cell carcinoma (OSCC). Material and Methods: The sample was divided into 2 groups: Cancer (36 subjects) and non-Cancer (12 subjects). The smokers' participants (36) were evaluated regarding their Nicotine dependence (ND) was assessed by the Fagerström test for cigarette dependence (FTCD). Questions regarding tobacco consumption like the number of cigarettes/day (CPD), duration of use, and pack-years were also evaluated. The Mann-Whitney and Spearman correlation tests were used at a significance level of 5%. Results: 48 participants were included, 32 men (66.7%), 36 smokers (75%) and 27 smokers with OSCC (56.3%). Samples of OSCC expressed more CYP1A1, CYP1B1, and CYP2A6. Especially, the CYP1B1 gene was significantly expressed in OSCC samples, regardless gender or tobacco use. No women expressed CYP2A6, as well as, non-smokers did not express the CYP1A1 and CYP2A6 genes. CYP1A1 gene was higher among men (P = 0.021). Conclusion: Lack of exposure to tobacco may justify the absence of CYP1A1 and CYP2A6 expression in non-smokers. The CYP1B1 gene was significantly expressed in the cancer presence despite gender or tobacco use. The assessment of ND and quantification of tobacco consumption are important instruments in monitoring smokers with benign oral lesions and, especially, in the presence of cancer.(AU)


Objetivo: A fumaça do tabaco é composta de substâncias químicas cancerígenas conhecidas como carcinógenos. Esses carcinógenos são metabolizados pelas enzimas da família do citocromo P450 (CYP). Nosso objetivo foi avaliar a correlação dos parâmetros do consumo de tabaco com a expressão de CYP1A1, CYP1B1 e CYP2A6 por qRT-PCR em amostras de carcinoma de células escamosas bucal (CCEB). Material e Métodos: A amostra foi dividida em 2 grupos: Câncer (36 indivíduos) e sem Câncer (12 indivíduos). Os participantes fumantes (36) foram avaliados quanto à dependência nicotínica (DN) pelo teste de Fagerström para dependência de cigarro (TFDC). Questões relacionadas ao consumo de tabaco como número de cigarros / dia (CPD), tempo de uso e anos-maço também foram avaliadas. Os testes de correlação de Mann-Whitney e Spearman foram utilizados com nível de significância de 5%. Resultados: foram incluídos 48 participantes, 32 homens (66,7%), 36 fumantes (75%) e 27 fumantes com CCEB (56,3%). Amostras de CCEB expressaram mais CYP1A1, CYP1B1 e CYP2A6. Especialmente, o gene CYP1B1 foi significativamente expresso em amostras de CCEB, apesar do sexo ou uso de tabaco. Nenhuma mulher expressou CYP2A6, assim como, não fumantes não expressaram os genes CYP1A1 e CYP2A6. O gene CYP1A1 foi maior entre os homens (P = 0,021). Conclusão: A falta de exposição pode justificar a ausência da expressão dos genes CYP1A1 e CYP2A6 entre não fumantes. O gene CYP1B1 foi significativamente expresso na presença de câncer, independentemente do sexo ou do uso de tabaco. A avaliação da DN e a quantificação do consumo de tabaco são importantes instrumentos no acompanhamento de fumantes com lesões bucais benignas e, principalmente, na presença de câncer (AU)


Subject(s)
Humans , Tobacco Use Disorder , Carcinoma , Carcinoma, Squamous Cell , Cytochrome P-450 CYP1A1 , Cytochrome P-450 CYP1B1 , Cytochrome P-450 CYP2A6
5.
Oncología (Ecuador) ; 31(3): 234-242, 30-diciembre-2021.
Article in Spanish | LILACS | ID: biblio-1352468

ABSTRACT

Se define carcinoma de cabeza y cuello (CCC) de primario desconocido al cuadro de adenopatía cervical en que, luego de examen físico, estudios de imágenes y panendoscopía con biopsias, no se encuentra el tumor primario pero sí la confirmación de malignidad de la adenomegalia. Son infrecuentes, por lo que estudios prospectivos que arrojen resultados estadísticamente significativos no están disponibles actualmente, y el tratamiento definitivo es aún motivo de controversia. Al ser la radioterapia un tratamiento dirigido es imprescindible definir adecuadamente los volúmenes blanco de tratamiento; es ideal el hallazgo del tumor primario, pero en muchos casos a pesar de un estudio escalonado, exhaustivo y multidisciplinar esto no se logra. Esto motiva el debate de qué regiones tratar, dosis, fraccionamiento y modalidad (exclusiva, adyuvante, en concurrencia). Hasta el momento el tratamiento de ganglios cervicales y mucosa de alto riesgo parece ser la estrategia con mejor control locorregional.


Head and neck carcinoma (HNC) of unknown primary is a clinical condition defined as a cervical adenopathy for which, after physical examination, imaging studies and panendoscopy with biopsies, the primary tumor is not found, but there is confirmed malignancy of the adenomegaly. It is infrequent, so prospective studies that yield statistically significant results are not currently available, and definitive treatment is still controversial. Since radiation therapy is a targeted treatment, it is essential to adequately define treatment target volumes; the discovery of the primary tumor is ideal, but in many cases, despite a phased, exhaustive and multidisciplinary study, this is not achieved. This motivates the debate on which regions to treat, dose, fractionation and modality (exclusive, adjuvant, concurrent). Until now, the treatment of high-risk cervical nodes and mucosa seems to be the strategy with the best locoregional control.


Subject(s)
Humans , Adult , Middle Aged , Aged , Radiotherapy , Head and Neck Neoplasms , Carcinoma, Squamous Cell , Lymphadenopathy
6.
Braz. j. oral sci ; 20: e210298, jan.-dez. 2021. ilus
Article in English | LILACS, BBO | ID: biblio-1252387

ABSTRACT

Oral squamous cell carcinoma (OSCC) is one of the most well-known malignancies that affect the human population worldwide. The early diagnosis and early intervention of OSCC help improve the survival rate of the patients. The tumour free surgical margins are a positive prognostic factor for recurrence-free survival. The molecular markers can be used to detect the tumour free surgical margins. Aim: The aim of the study is to evaluate the expression of p53 & Cyclin D1 marker in resected surgical apparently clear margins and to correlate the p53 & Cyclin D1 expression with clinicopathological characteristics and patient outcome. Methods: The study population included retrospective cases of OSCC with apparently clear margins (2017-18) n=10 and Clinicopathological variables relevant to survival analysis were recorded. Finally, two margins were selected from each case, a total of 20 margins were included in this study. Paraffin-embedded wax blocks retrieved and tissue sections were made. Expression of cyclin D1 and p 53 was assessed by the immunohistochemical staining procedure Results: Positive expressions Cyclin D1 in 40% of mild dysplasia margins and 60% in clearance adequate margins were present. p53 expression was seen in 16% of mild dysplasia margins and 84% in clearance adequate margins. The expression of p53 and Cyclin D1 molecular markers are noted in the basal & parabasal layer of epithelium. Conclusion: Molecular markers could play a more reliable method for the assessment of dysplasia at the margins


Subject(s)
Humans , Male , Female , Carcinoma, Squamous Cell , Tumor Suppressor Protein p53 , Cyclin D1
7.
Rev. bras. ciênc. vet ; 28(4): 181-183, out./dez. 2021. il.
Article in Portuguese | LILACS | ID: biblio-1363186

ABSTRACT

O carcinoma espinocelular é uma neoplasia maligna dos ceratinócitos que tem como principal causa a exposição a raios ultravioletas. Os felinos constituem a espécie doméstica mais susceptível para o aparecimento desta neoplasia, sendo a face a região mais comum de acometimento, incluindo áreas do plano nasal, pina e pálpebras. No presente trabalho relata-se um felino, sem raça definida, fêmea, 12 anos de idade, 3kg, castrada, de pelagem branca, que foi atendida apresentando lesão extensa de caráter ulcerativo em região periocular de globo ocular esquerdo, com evolução de aproximadamente três meses, envolvendo ambas as pálpebras, superior e inferior. Após realização de avaliação clínica, os achados obtidos por meio da anamnese juntamente com o exame citológico, sugeriram como principal diagnóstico o carcinoma espinocelular. Para tratamento foi preconizado remoção cirúrgica, na qual demonstrou boa efetividade para tratamento dessa afecção. Após, o material foi enviado para análise histopatológica, sendo confirmado o diagnóstico. O paciente apresentou boa recuperação e evolução clínica do quadro, não apresentando nenhuma complicação no decorrer do tratamento. O prognóstico para este caso foi considerado bom, uma vez que não havia indícios de metástase no momento da realização dos exames complementares, e as margens da neoplasia se encontravam livres de células neoplásicas.


Squamous cell carcinoma is a malignant neoplasm of keratinocytes whose main cause is exposure to ultraviolet rays. The original felines are the domestic species most susceptible to the onset of this neoplasm, the face being the most common region of involvement, including areas of the nasal plane, pinna and eyelids. In the present study, we report a feline, mixed breed, female, 12 years old, 3kg, neutered, with white fur, who was treated for an enlarged ulcerative lesion in the periocular region of the left eyeball, with an evolution of approximately three months. involving both the upper and lower eyelids. After conducting a clinical valuation, the findings obtained through the anamnesis together with the cytological examination, suggested squamous cell carcinoma as the main diagnosis. For treatment before surgical removal, in the qualification good effectiveness to treat this condition. Afterwards, the material sent for histopathological analysis, the diagnosis being confirmed. The patient shows good recovery and clinical evolution of the condition, there is no complication or complication during the treatment. The prognosis for this case was considered good, since there was no evidence of metastasis at the time of the complementary tests, and as the neoplasia margins were free of neoplastic cells.


Subject(s)
Animals , Cats , Neoplasms , Surgery, Veterinary , Carcinoma, Squamous Cell , Cat Diseases , Corneal Keratocytes
8.
Rev. cuba. cir ; 60(3): e1074, 2021. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1347384

ABSTRACT

Introducción: El cáncer de esófago es una de las neoplasias más agresivas del tracto digestivo, presenta alta morbilidad y mortalidad a nivel mundial. Objetivo: Determinar las características clínico epidemiológicas de los pacientes con cáncer de esófago y su oportunidad del tratamiento. Métodos: Se realizó una investigación básica de tipo descriptiva y retrospectiva en los pacientes con cáncer de esófago hospitalizados en el Instituto Nacional de Oncología y Radiobiología durante el período enero 2016 - enero 2017. Se obtuvieron los datos necesarios de las historias clínicas individuales de los casos que cumplieron los criterios de inclusión. Se aplicaron frecuencias absolutas y porcentajes para permitir los análisis estadísticos de variables seleccionadas. Resultados: El diagnóstico en las edades entre 60 y 69 años fue más frecuente, con el 44,8 por ciento de los casos; el 73,7 por ciento eran hombres y el 52,3 por ciento de la raza negra. En el 71,0 por ciento se encontró antecedentes de esofagitis de reflujo; 78,9 por ciento eran fumadores y 52,6 por ciento bebedores abusivos. La disfagia fue el síntoma más común y el carcinoma epidermoide se presentó con mayor frecuencia alcanzándose el 82,1 por ciento de la muestra estudiada. La mayoría de los casos se diagnosticó en estadio III. Conclusiones: Esta neoplasia constituye un problema de salud en Cuba, se incrementa su incidencia en los últimos años. Se recomienda aplicar el programa de detección y diagnóstico precoz de la enfermedad(AU)


Introduction: Esophageal cancer is one of the most aggressive neoplasms of the digestive tract; it accounts for high morbidity and mortality worldwide. Objective: To determine the clinical-epidemiological characteristics of patients with esophageal cancer and their chances for treatment. Methods: A descriptive and retrospective basic research was carried out, during the period from January 2016 to January 2017, in patients with esophageal cancer hospitalized at the National Institute of Oncology and Radiobiology. The necessary data were obtained from the individual medical records of the cases. that met the inclusion criteria. Absolute frequencies and percentages were applied for the statistical analyses of the selected variables. Results: The diagnosis at ages between 60 and 69 years was more frequent, accounting for 44.8 percent of the cases. 73.7 percent were men and 52.3 percent were of black race. A history of reflux esophagitis was found in 71.0 percent. 78.9 percent were smokers and 52.6 percent were abusive drinkers. Dysphagia was the most common symptom, while squamous cell carcinoma presented more frequently, reaching 82.1 percent of the sample studied. Most of the cases were diagnosed in stage III. Conclusions: This neoplasm is a health concern in Cuba; its incidence has increased in recent years. It is recommended to apply the program of early diagnosis and detection of the disease(AU)


Subject(s)
Humans , Male , Middle Aged , Aged , Esophageal Neoplasms/epidemiology , Carcinoma, Squamous Cell/therapy , Esophagitis, Peptic/drug therapy , Early Diagnosis , Medical Records , Epidemiology, Descriptive , Retrospective Studies , Treatment Adherence and Compliance
9.
Medicentro (Villa Clara) ; 25(3): 504-512, 2021. graf
Article in Spanish | LILACS | ID: biblio-1340198

ABSTRACT

RESUMEN El riesgo de cáncer de ovario a lo largo de la vida de una mujer es menor de 2 %, y durante la vida reproductiva es de 0,01 %. La incidencia de cáncer de ovario durante el embarazo es de 1: 10 000 - 100 000 casos. La mayoría de las masas anexiales en la gestación son asintomáticas. El diagnóstico se realiza frecuentemente mediante un hallazgo casual en la ecografía obstétrica; se ha estimado una incidencia de 0,2 - 2 %, y de estas, 1 - 6 % son malignas. El carcinoma epidermoide desarrollado en un teratoma quístico maduro es una rara neoplasia que representa, aproximadamente, el 2 % de los tumores primarios del ovario. Se presenta una paciente con diagnóstico accidental de teratoma ovárico maduro con diferenciación de carcinoma epidermoide, durante la realización de una cesárea.


ABSTRACT The risk of ovarian cancer throughout a woman's life is less than 2%, and during reproductive life it is 0.01%. The incidence of ovarian cancer during pregnancy is 1: 10,000 - 100,000 cases. Most adnexal masses in pregnancy are asymptomatic. Diagnosis is frequently made by casual finding on obstetric ultrasound; an incidence of 0.2 - 2% has been estimated, and 1 - 6% of them are malignant. Squamous cell carcinoma developed in a mature cystic teratoma is a rare neoplasm that represents approximately 2% of primary ovarian tumors. We present a patient with an accidental diagnosis of mature ovarian teratoma with differentiation of squamous cell carcinoma, during a cesarean section.


Subject(s)
Pregnancy Complications, Neoplastic , Teratoma , Carcinoma, Squamous Cell
10.
Oncol. (Guayaquil) ; 31(2): 104-113, 31 de agosto 2021.
Article in Spanish | LILACS | ID: biblio-1284428

ABSTRACT

Introducción: La maxilectomía es la intervención quirúrgica recomendada para la exéresis de neoplasias faciales, esta técnica comprende la resección de los maxilares y de estructuras anatómicas adyacentes, y conlleva diferentes grados de alteración funcional. La reconstrucción de defectos medio faciales re-presenta un gran desafío, se utilizan diferentes técnicas desde el uso de prótesis obturadoras, colgajos locales, hasta colgajos libres, con la finalidad de la mayor recuperación de funciones sofisticadas como el habla, la deglución y la ventilación en vía área superior. Reporte de caso: Paciente hombre de 89 años de edad con 4 meses de evolución de masa tumoral decrecimiento progresivo y constante en la mitad del paladar superior y se extiende tomando el maxilar superior derecho. Al examen físico se observa la cavidad con deficiente cuidado de las piezas dentales y una masa tumoral de más de 15 cm de diámetro que deforma la cavidad oral. Evolución: Se realizó tumorectomía y vaciamiento ganglionar con preservación de nervio espinal, hipogloso, lingual, musculo esternocleidomastoideo, vena yugular interna, vena y arteria facial. Se realizó una reconstrucción de neopaladar con colgajo nasogeniano. La patología definitiva recibida en días posteriores reportó un carcinoma escamo celular pobremente diferenciado de estirpe epitelial con borde quirúrgico positivo para el tumor. Dentro del período postoperatorio el paciente presentó una neumonía asociada a los cuidados de la salud, acompañada con déficit neurológico, el cuadro involucionó hasta sepsis de origen pulmonar y fallece 21 días posteriores a la cirugía. Conclusiones: El crecimiento acelerado de una masa del maxilar en este paciente se asoció con la presencia de un carcinoma escamo celular pobremente diferenciado.


Introduction: Maxillectomy is the recommended surgical intervention for the excision of facial neoplasms. This technique includes the resection of the jaws and adjacent anatomical structures, and en-tails different degrees of functional alteration. The reconstruction of mid-facial defects represents a great challenge, different techniques are used from the use of obturator prostheses, local flaps, to free flaps, with the aim of greater recovery of sophisticated functions such as speech, swallowing and ventilation in via upper area. Case report: An 89-year-old male patient with a 4-month evolution of a tumor mass with progressive and constant growth in the middle of the upper palate and extending taking the right upper jaw. Physical examination revealed the cavity withpoor dental care and a tumor mass of more than 15 cm in diameter that deformed the oral cavity. Evolution: A lumpectomy and lymph node dissection were performed with preservation of the spinal, hypoglossal, lingual, sternocleidomastoid muscle, internal jugular vein, vein and facial artery. A neo-palatal reconstruction with a nasolabial flap was performed. The definitive pathology received in later days reported a poorly differentiated squamous cell carcinoma of epithelial lineage with a positive surgical border for the tumor. Within the postoperative period, the patient presented pneumonia associated with health care, accompanied by neurological deficit, the condition regressed to sepsis of pulmonary origin and died 21 days after surgery. Conclusions: The accelerated growth of a maxillary mass in this patient was associated with the pres-ence of a poorly differentiated squamous cell carcinoma.


Introdução: A maxilectomia é a intervenção cirúrgica recomendada para a excisão de neoplasias faciais, esta técnica inclui a ressecção da mandíbula e das estruturas anatômicas adjacentes e acarreta diferentes graus de alteração funcional. A reconstrução dos defeitos médios da face representa um grande desafio, diferentes técnicas são utilizadas desde o uso de próteses obturadoras, retalhos locais, até retalhos livres, com o objetivo de maior recuperação de funções sofisticadas como fala, deglutição e ventilação em via. . Relato do caso: Paciente do sexo masculino, 89 anos, com evolução de 4 meses de massa tumoral progressiva e constante decrescente em meio palato superior e extensão em maxilar superior direito. Ao exame físico, observa-se cavidade com mau atendimento odontológico e massa tumoral de mais de 15 cm de diâmetro que forma a cavidade oral. Evolução: Realizada lumpectomia e dissecção dos linfonodos com preservação da coluna vertebral, hipoglosso, nervo lingual, músculo esternocleidomastóideo, veia jugular interna, veia e artéria facial. Foi realizada reconstrução neo-palatina com retalho nasolabial. A patologia definitiva recebida em dias posteriores relatou um carcinoma de células escamosas pouco diferenciado de linhagem epitelial com uma borda cirúrgica positiva para o tumor. No pós-operatório, o paciente apresentou pneumonia associada aos cuidados de saúde, acompanhada de déficit neurológico, o quadro regrediu para sepse de origem pulmonar e faleceu 21 dias após a cirurgia. Conclusões: O crescimento acelerado de uma massa maxilar neste paciente foi associado à presença de um carcinoma espinocelular pouco diferenciado.


Subject(s)
Humans , Aged, 80 and over , Carcinoma, Squamous Cell , Free Tissue Flaps , Case Reports , Maxillary Neoplasms , Mouth
11.
An. bras. dermatol ; 96(3): 263-277, May-June 2021. tab, graf
Article in English | LILACS | ID: biblio-1285080

ABSTRACT

Abstract Mohs micrographic surgery is a specialized form of skin cancer surgery that has the highest cure rates for several cutaneous malignancies. Certain skin cancers can have small extensions or "roots" that may be missed if an excised tumor is serially cross-sectioned in a "bread-loaf" fashion, commonly performed on excision specimens. The method of Mohs micrographic surgery is unique in that the dermatologist (Mohs surgeon) acts as both surgeon and pathologist, from the preoperative considerations until the reconstruction. Since Dr. Mohs's initial work in the 1930s, the practice of Mohs micrographic surgery has become increasingly widespread among the dermatologic surgery community worldwide and is considered the treatment of choice for many common and uncommon cutaneous neoplasms. Mohs micrographic surgery spares the maximal amount of normal tissue and is a safe procedure with very few complications, most of them managed by Mohs surgeons in their offices. Mohs micrographic surgery is the standard of care for high risks basal cell carcinomas and cutaneous squamous cell carcinoma and is commonly and increasingly used for melanoma and other rare tumors with superior cure rates. This review better familiarizes the dermatologists with the technique, explains the difference between Mohs micrographic surgery and wide local excision, and discusses its main indications.


Subject(s)
Humans , Skin Neoplasms/surgery , Carcinoma, Basal Cell/surgery , Carcinoma, Squamous Cell/surgery , Melanoma/surgery , Mohs Surgery
13.
Rev. med. Risaralda ; 27(1): 21-27, ene.-jun. 2021. graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1280489

ABSTRACT

Resumen Objetivo: Analizar la tendencia de la incidencia de cáncer de cuello uterino invasivo en mujeres entre 20 y 39 años en Manizales, Colombia, 2003-2018. Materiales y métodos: Estudio observacional, descriptivo, retrospectivo, de base poblacional. Se evaluaron las tendencias temporales en la incidencia (con referencia a Población Segi, por 100.000 mujeres/año) utilizando la regresión de Joinpoint (Joint Point Regression Program v. 4.7.0.0 del National Cancer Institute de los EEUU) para estimar los cambios porcentuales anuales y años de variación significativa en las tendencias. Resultados: Se realizó el estudio con un total de 116 pacientes, cuya edad promedio al momento del diagnóstico fue 33.7 años. Sólo se hallaron 3 casos de cáncer invasivo en mujeres entre 20 y 25 años. El mayor número de casos se presentó en el grupo de mujeres de 35 a 39 años. El tipo histológico más frecuente fue el carcinoma escamocelular. El 45.6% de los casos se diagnosticaron en etapas tempranas. El Cambio Porcentual Anual Promedio calculado mediante el modelo de regresión Joinpoint mostró un descenso de 7,9% en la tasa de incidencia entre 2003 y 2018, con un punto de quiebre en el año 2010. Conclusiones: El cáncer de cuello uterino en Manizales tiene escasa frecuencia en mujeres menores de 25 años y para edades entre 20 y 39 años muestra una tendencia a la disminución en el tiempo, en particular desde el año 2010 en adelante.


Abstract Objective: This article analyzes the invasive uterine cancer occurrences in women between 20 and 39 years old in Manizales, Colombia, 2003-2018. Materials and methods: Observational, descriptive, retrospective, population-based study. Temporary trends of occurrences were evaluated (as referring to Population Segi, per 100,000 women / year) by using the Joinpoint regression (Joint Point Regression Program v. 4.7.0.0 of the National Cancer Institute of the USA), in order to estimate annual percentage changes and number of years of significant variation in trends. Results: The study was carried out with a total of 116 patients, whose average age at the time of diagnosis was 33.7 years. Only 3 invasive cancer cases were found in women between the ages of 20 and 25. The greatest number of cases occurred in the group of women between 35 and 39 years old. The most frequent histological type was squamous cell carcinoma. 45.6% of cases were diagnosed in early stages. The Average Annual Percentage Change calculated using the Joinpoint regression model showed a 7.9% decrease in the incidence rate between 2003 and 2018, with a breaking point in 2010. Conclusions: Cervical cancer in Manizales is infrequent in women under the age of 25; and, for ages between 20 and 39, it has shown a tendency to decrease over time, particularly from 2010 onwards.


Subject(s)
Humans , Female , Adult , Uterine Neoplasms , Uterine Cervical Neoplasms , Carcinoma, Squamous Cell , Colombia , Diagnosis , Joints , Methods , Neoplasms
14.
Rev. colomb. anestesiol ; 49(2): e501, Apr.-June 2021. graf
Article in English | LILACS, COLNAL | ID: biblio-1251503

ABSTRACT

Abstract Introduction Neuropathic pain is present in up to 40 % of all cancer patients. A considerable number of patients fail to achieve enough pain relief with conventional treatment, which is why therapeutic alternatives such as spinal cord stimulation should be considered. Case description and results This is the case of a female patient with chronic neuropathic pain secondary to a partial femoral nerve injury sustained during resection and lymph node dissection surgery with curative intent for a large stage II cell squamous cell carcinoma T2N0M0, localized in the right popliteal fossa. The patient presented with difficult to manage chronic neuropathic pain, despite receiving multiple oral analgesics and nerve blocks. A medullary neurostimulator was implanted that relieved the patient's pain intensity in up to 80%, in addition to improved function and quality of life. Conclusions Spinal cord stimulation is considered an effective neuromodulatory intervention which has shown satisfactory results in the treatment of various types of refractory chronic pain in cancer patients, including neuropathic pain.


Resumen Introducción El dolor neuropático está presente hasta en el 40 % de los pacientes con cáncer. Un número considerable de pacientes no logran un alivio suficiente del dolor con el tratamiento convencional, por lo cual deben considerarse alternativas terapéuticas como la estimulación de la médula espinal. Descripción del caso y resultados Caso de una paciente con dolor neuropático crónico secundario a lesión parcial de nervio femoral durante cirugía de resección y vaciamiento ganglionar con objetivos curativos de carcinoma escamocelular de célula grande T2N0M0 estadio II, localizado en la fosa poplítea derecha, quien cursó con dolor neuropático crónico de difícil manejo a pesar de recibir múltiples analgésicos orales y bloqueos nerviosos. Se implantó un neuroestimulador medular con lo cual se logró un alivio hasta del 80 % en intensidad de dolor de la paciente, además de una mejoría de su funcionalidad y calidad de vida. Conclusiones La estimulación de la médula espinal se considera una intervención neuromoduladora eficaz, que ha demostrado resultados satisfactorios para tratar diversas formas de dolor crónico refractario en los pacientes con cáncer, incluido el dolor neuropático.


Subject(s)
Humans , Female , Middle Aged , Carcinoma, Squamous Cell , Chronic Pain , Pain Management , Spinal Cord Stimulation , Lymph Node Excision , Neoplasms , Quality of Life , Therapeutics , Cells , Femoral Nerve , Analgesics , Nerve Block , Neuralgia
15.
Rev. colomb. gastroenterol ; 36(supl.1): 112-115, abr. 2021. graf
Article in Spanish | LILACS | ID: biblio-1251558

ABSTRACT

Resumen La lengua negra pilosa (LNP) es una alteración benigna caracterizada por coloración oscura e hipertrofia con hiperqueratosis en las papilas filiformes de la superficie de la lengua. Han sido implicados varios factores intrínsecos y extrínsecos como causales, entre ellos la pobre higiene dental, el tabaquismo, la ingesta de bebidas oscuras, el uso de antibióticos y algunas patologías malignas. Reportamos el caso de un paciente con carcinoma escamocelular avanzado de esófago medio conjunto con LNP, asociación no encontrada previamente en la literatura.


Abstract Black hairy tongue (BHT) is a benign disorder characterized by dark coloration and hypertrophy with hyperkeratosis in filiform papillae on the surface of the tongue. Several intrinsic and extrinsic factors, including poor dental hygiene, smoking, drinking dark beverages, indiscriminate use of antibiotics, and some malignant diseases, have been proposed as potential causes. This is the case of a patient with advanced squamous cell carcinoma of the mid esophagus in conjunction with BHT, a previously unknown association.


Subject(s)
Humans , Female , Aged , Tongue , Carcinoma, Squamous Cell , Esophagus , Oral Hygiene , Association , Tobacco Use Disorder , Smoking
16.
Dermatol. argent ; 27(1): [3-11], ene.-mar. 2021. il, tab
Article in Spanish | LILACS, BINACIS | ID: biblio-1359875

ABSTRACT

El linfoma de células T epidermotropo agresivo CD8+ o linfoma de Berti es un subtipo infrecuente de los linfomas primarios cutáneos, descripto en 1999. Aún se considera una entidad provisional en la última clasificación de linfomas cutáneos primarios de la Organización Mundial de la Salud. Predomina en los hombres adultos y se manifiesta con pápulas, placas hiperqueratósicas y tumores ulcerados, diseminados y de inicio agudo. En la histopatología, es característica la presencia de un infiltrado de linfocitos atípicos medianos CD8 +, con epidermotropismo de patrón pagetoide. Tiene un comportamiento agresivo y es de mal pronóstico, con una sobrevida a los 5 años menor del 40%. Si bien esta entidad es un reto terapéutico, el tratamiento inicial se basa en poliquimioterapia y/o trasplante de células hematopoyéticas.


The aggressive epidermotropic CD8+ T-cell lymphoma or Berti's lymphoma, is a rare subtype of primary cutaneous lymphomas, first described in 1999. It is still considered a provisional entity by the latest World Health Organization classification of cutaneous lymphomas. Patients are commonly adults with a male predominance and it is characterized by widespread rapid evolving papules, hyperkeratotic annular plaques and ulcerated tumors. Histopathologicfindingsarecharacteristic,withaninfiltrate of medium-sized CD8+ atypical lymphocytes involving the epidermis with a pagetoid pattern. Ithasanaggressivebehaviorandtheprognosisispoor with a 5-yearsurvival less than 40%. Instead these disease represents a therapeutic challenge, the initial treatment consists on polychemotherapy and hematopoietic stem cell transplantation.


Subject(s)
Humans , Skin Neoplasms , Lymphoma, Primary Cutaneous Anaplastic Large Cell/diagnosis , Carcinoma, Squamous Cell , Drug Therapy, Combination , Lymphoma, Primary Cutaneous Anaplastic Large Cell/therapy
18.
São Paulo; s.n; 2021. 69 p. ilust, tabelas.
Thesis in Portuguese | LILACS, Inca | ID: biblio-1222633

ABSTRACT

O carcinoma epidermóide (CEC) é a neoplasia maligna mais frequente em cavidade oral, representando aproximadamente 90% de todos os cânceres da boca. Esta neoplasia tem origem no epitélio de revestimento bucal, e a depender do subtipo e da sua localização, pode apresentar tendência marcante de invasão do tecido ósseo adjacente. Existe uma interação entre osso e neoplasia, e atenção principal deve ser dada aos reguladores relacionados à osteoclastogênese. As moléculas RANK/RANKL/OPG são fundamentais neste processo, e juntamente com a IL-6, promovem formação e ativação de osteoclastos, com consequente promoção da invasão mandibular. Neste sentido, o objetivo do estudo foi avaliar as características clínicas, radiográficas e histopatológicas dos pacientes submetidos a mandibulectomia para tratamento do carcinoma epidermóide oral, e correlacionar/associar com a expressão gênica na neoplasia de marcadores reguladores da invasão óssea. Foi realizado um estudo retrospectivo de pacientes tratados com algum tipo de mandibulectomia no A.C. Camargo Cancer Center durante o período de1990 a 2015. Um total de 220 casos foi selecionado, e destes, foram extraídos 40 casos, posteriormente submetidos as etapas de análise molecular. A análise molecular foi realizada por PCR quantitativa em tempo real (qPCR), por meio da mensuração de mRNA para os genes estudados, calibrados pelo gene endógeno GAPDH e por um pool de controle composto por RNA não neoplásico. Dos 220 pacientes incluídos na pesquisa, 161 eram do sexo masculino, a idade média foi de 60 anos, e 53,6% eram etilistas e 66,8% tabagistas. A invasão óssea da mandíbula foi observada através do laudo anatomopatológico em 77 pacientes, e foi associada à presença de margens comprometidas/exíguas e a localização da lesão (gengiva inferior, rebordo e região retro-molar). Ao final do estudo, 127 pacientes foram a óbito, e a média de sobrevida foi de 96,92 meses. Parâmetros clínicos, dos exames de imagem e histopatológicos foram significantemente associados com a sobrevida global, principalmente - tamanho do tumor, infiltração perineural, invasão óssea (imagem e anatomopatológico). Na análise multivariada, apenas invasão óssea e infiltração perineural aumentaram risco de óbito nos pacientes. Na análise molecular de expressão gênica foi possível avaliar que das 36 amostras do gene RANK, 16 eram downreguladas. Para RANKL, das 37 amostras, 28 estavam super expressas. Das 35 do gene da IL-6, 19 amostras eram downreguladas. E por fim, das 37 amostras do gene da OPG, 16 tinham expressão normal. A avaliação de associação entre a expressão dos genes analisados demostrou que o gene da IL-6 está associado a presença de invasão óssea, e que existe uma associação significativa entre as expressões do RANK, RANKL, OPG e IL-6. Maior sobrevida dos pacientes foi associada significativamente a uma expressão mais baixa de IL-6 (108,5 meses). Conclui-se que a invasão mandibular interfere na sobrevida global dos pacientes, e que este mecanismo de invasão óssea está associado com a expressão gênica dos genes RANK, RANKL, OPG e IL-6. A IL-6 downregulada nas células malignas está associada com maior sobrevida


Oral Squamous Cell Carcinoma (OSCC) is the most frequent malignant neoplasm in the oral cavity, representing 90% of all oral cancers. The tumor usually arises within the epithelium lining of the oral cavity, and in cases of certain subtype or location may reveal a tendency for invasion of the surrounding bone tissue. There is a relation between tumor and bone, and more attention should be given to the events related to osteoclastogenesis. The molecules RANK/RANKL/OPG are crucial in this process, and together with IL-6, promotes the formation and activation of osteoclasts, with influence on the mandibular invasion process. In this way, the aim of this study was to evaluate the clinical, demographic, imaging, and histopathology data of patients diagnosed with OSCC treated by mandibulectomy. Also, analyze the gene expression condition of the genes RANK, RANKL, OPG, and IL-6 on the tumor. A retrospective study was conducted on patients treated with mandibulectomy at the A.C. Camargo Cancer Center, between 1990 and 2015. Two hundred and twenty cases were retrieved, and 40 of them were selected for gene expression analysis. The mRNA expressions of the selected genes were determined by real-time polymerase chain reaction, extracted from the tumor, and calibrated by endogenous GAPDH and a control pool of non-neoplastic RNA. Among 220 patients, 161 were male, with a mean age of 60 years. 53.6% were alcohol users and 66.8% tobacco users. Mandible bone invasion was reported on 77 histopathology reports and associated with compromised margins and the site of the lesion (gingiva, alveolar ridge, and retromolar region). One hundred and twenty-seven patients died of the disease, and the mean overall survival rate was 96.92 months. Overall survival was associated with clinical, imaging, and pathological parameters, especially, tumor size, perineural growth, and bone invasion (both imaging and pathologic). Multivariate analysis revealed higher risk of death only to bone invasion and perineural growth. Gene expression analysis revealed - 16 downregulation of 36 RANK samples evaluated; 28 overexpression of 37 RANKL samples evaluated; 19 downregulation of 35 IL-6 analyzed, and 16 normal expression of 37 OPG samples evaluated. Association analysis revealed that IL-6 expression is associated with bone invasion, and there is also a significant association among the expression of RANK, RANKL, OPG, and IL-6. Higher overall survival rate was associated with an IL-6 down expression (108;5 months). In conclusion, mandibular invasion interferes with the patient's overall survivall rate, and the bone invasion mechanism is associated with gene expression of RANK, RANKL, OPG, and IL-6 on the tumor. IL-6 in downregulation expressed by malignant cells is associated with higher overall survivor rates


Subject(s)
Humans , Female , Pregnancy , Adult , Middle Aged , Prognosis , Carcinoma, Squamous Cell , Interleukin-6 , Receptor Activator of Nuclear Factor-kappa B , Osteoprotegerin , Mandible
19.
Braz. j. med. biol. res ; 54(8): e10877, 2021. tab, graf
Article in English | LILACS | ID: biblio-1249331

ABSTRACT

Esophageal squamous cell carcinoma (ESCC) is among the ten most frequent and deadly cancers, without effective therapies for most patients. More recently, drugs targeting deregulated growth factor signaling receptors have been developed, such as HGF-MET targeted therapy. We assessed MET and HGF genetic alterations and gene and protein expression profiles in ESCC patients from the Brazilian National Cancer Institute and publicly available datasets, as well as the intratumor heterogeneity of the alterations found. Our analyses showed that HGF and MET genetic alterations, both copy number and mutations, are not common in ESCC, affecting 5 and 6% of the cases, respectively. HGF showed a variable mRNA expression profile between datasets, with no alterations (GSE20347), downregulation (GSE45670), and upregulation in ESCC (our dataset and GSE75241). On the other hand, MET was found consistently upregulated in ESCC compared to non-tumor surrounding tissue, with median fold-changes of 5.96 (GSE20347), 3.83 (GSE45670), 6.02 (GSE75241), and 5.0 (our dataset). Among our patients, 84% of the tumors showed at least a two-fold increase in MET expression. This observation was corroborated by protein levels, with 55% of cases exhibiting positivity in 100% of the tumor cells. Intratumor heterogeneity was evaluated in at least four tumor biopsies from five patients and two cases showed a consistent increase in MET expression (at least two-fold) in all tumor samples. Our data suggested that HGF-MET signaling pathway was likely to be overactivated in ESCC, representing a potential therapeutic target, but eligibility for this therapy should consider intratumor heterogeneity.


Subject(s)
Humans , Esophageal Neoplasms/genetics , Carcinoma, Squamous Cell/genetics , Esophageal Squamous Cell Carcinoma/genetics , Head and Neck Neoplasms , Brazil , Gene Expression Regulation, Neoplastic , Proto-Oncogene Proteins c-met/genetics , Proto-Oncogene Proteins c-met/metabolism , Cell Line, Tumor
20.
Braz. j. med. biol. res ; 54(5): e10093, 2021. tab, graf
Article in English | LILACS | ID: biblio-1153556

ABSTRACT

The aim of this study was to explore the effect of hsa_circ_0002162 on regulating cell proliferation, apoptosis, and invasion, and investigate its potential target microRNA (miRNA) in tongue squamous cell carcinoma (TSCC). Hsa_circ_0002162 expression was detected in human TSCC cell lines and human oral keratinocytes (HOK) cell line. Cell proliferation, apoptosis, invasion, and candidate target miRNA expressions were detected in hsa_circ_0002162 knockdown-treated CAL-27 cells and hsa_circ_0002162 overexpression-treated SCC-9 cells. In the rescue experiment, miR-33a-5p knockdown plasmid was transfected into hsa_circ_0002162 knockdown-treated CAL-27 cells, while miR-33a-5p overexpression plasmid was transfected into hsa_circ_0002162 overexpression-treated SCC-9 cells. Subsequently, cell proliferation, apoptosis, and invasion were detected, and then luciferase reporter assay was performed. Hsa_circ_0002162 expression was increased in human TSCC cell lines SCC-9, CAL-27, HSC-4, and SCC-25 compared with HOK. In CAL-27 cells, hsa_circ_0002162 knockdown inhibited cell proliferation and invasion and promoted apoptosis. In SCC-9 cells, hsa_circ_0002162 overexpression enhanced cell proliferation and invasion and suppressed apoptosis. Furthermore, a negative regulation of hsa_circ_0002162 on miR-33a-5p (but not miR-302b-5p and miR-545-5p) was observed. In the rescue experiment, miR-33a-5p knockdown increased cell proliferation and invasion, and decreased apoptosis in hsa_circ_0002162 knockdown-treated CAL-27 cells, whereas miR-33a-5p overexpression decreased cell proliferation and invasion, but increased apoptosis in hsa_circ_0002162 overexpression-treated SCC-9 cells. The luciferase reporter assay showed the direct binding of hsa_circ_0002162 to miR-33a-5p. In conclusion, hsa_circ_0002162 had an important role in malignant progression of TSCC through targeting miR-33a-5p.


Subject(s)
Humans , Tongue Neoplasms/genetics , Carcinoma, Squamous Cell/genetics , MicroRNAs/genetics , Tongue , Cell Line, Tumor , RNA, Circular
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