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1.
Rev. cienc. cuidad ; 19(2): 31-39, 2022.
Article in Spanish | LILACS, BDENF, COLNAL | ID: biblio-1373526

ABSTRACT

Objetivo: Determinar el nivel de incertidumbre de las usuarias que acuden a la toma de ci-tología cérvico uterina según la teoría de Mishel. Materiales y métodos: Estudio de corte transversal de tipo descriptivo, realizado durante el año 2019 a partir de una muestra por conveniencia; es no probabilística y está conformada por 151 mujeres a quienes se les aplicó la Escala de Incertidumbre en la enfermedad de Mishel adaptada para procedimientos de diagnósticos en Colombia en el año 2017 por la enfermera Leidy Yazmin Díaz Moreno. Tiene validez de contenido de 0.861 y Alfa de Cronbach de 0.90. Además, se realizó un análisis univariado a través de frecuencias y distribución porcentual para las característi-cas sociodemográficas. Para obtener los niveles de incertidumbre se efectuó la sumatoria de ítems y chi cuadrado a fin de identificar la asociación. Resultados: La edad promedio fue de 39 años; el 17,3% de mujeres manifestó acudir a la toma de la citología por primera vez. Aproximadamente el 95% de las mujeres presentó niveles de incertidumbre alto o modera-do. Conclusiones: La incertidumbre provocada por un posible diagnóstico de cáncer, hace que las personas evalúen inadecuadamente una situación estresante como lo es la toma de citología cérvico uterina; es por eso que, la aplicación de la teoría de Mishel lleva a que se identifiquen las primeras percepciones del paciente que servirán para realizar un plan de cuidado enfocado en controlar esta situación y lograr que la persona o familiar afronten y se adapten a la enfermedad.


Objective: Determine the uncertainty level of the user that goes to the taking of cervical cytol-ogy according to the Mishel theory. Materials and methods: Cross-sectional study of descrip-tive type, realized during the 2019 year starting at a convenience sampling, non-probabilistic formed by 151 women's who was apply the Uncertainty in Illness Scale of Mishel adapted to diagnosis procedures in Colombia in 2017 by the nurse Leidy Yazmisn Díaz Moreno. It has conten validity of 0.861 and Cronbach's alpha of 0.9 Was realized a univariate analysis through frequencies and percent distribution for the sociodemographic characteristics and to obtain the levels of uncertainty was carried out the summation of items and chi ­ square to identify association. Results: The average age was 39 years, the 17,3% of women expressed go to the cytology by first time. Approximately the 95% of the women presented level of uncertainty high or moderate. Conclusion: The uncertainty caused for a possible terrible di-agnosis of cancer, can make that the persons evaluate inappropriately a stressful situation like the taking of the cervical cytology; that's why, the application of the Mishel Theory carry to identify the first perceptions of the patient to realize a care plan focus to control this situation and later reach that the person or familiar confront and adapt to the illness


Objetivo: Determinar o nível de incerteza dos clientes que freqüentam a citologia cervical de acordo com a teoria de Mishel. Materiais e métodos: Estudo descritivo transversal, realizado durante 2019 com base em uma amostra de conveniência; é não-probabilístico e é composto por 151 mulheres às quais foi aplicada a Escala de Incerteza Mishel adaptada para procedimentos diagnósticos na Colômbia em 2017 pela enfermeira Leidy Yazmin Díaz Moreno. Tem validade de conteúdo de 0,861 e o alfa do Cronbach de 0,90. Além disso, foi realizada uma análise uni-variada através de freqüências e distribuição percentual para as características sociodemográ-ficas. Para obter os níveis de incerteza, foi utilizada a soma dos itens e o qui-quadrado para iden-tificar a associação. Resultados: A idade média foi de 39 anos; 17,3% das mulheres relataram visitas citológicas pela primeira vez. Aproximadamente 95% das mulheres tinham níveis de incerteza altos ou moderados. Conclusões: A incerteza causada por um possível diagnóstico de câncer faz com que as pessoas avaliem inadequadamente uma situação estressante como a citologia cervical; portanto, a aplicação da teoria de Mishel leva à identificação das primeiras percepções do paciente que servirão para fazer um plano de cuidado focalizado no controle desta situação e fazer com que a pessoa ou membro da família enfrente e se adapte à doença


Subject(s)
Cell Biology , Attention , Uncertainty , Diagnosis , Education, Nursing , Secondary Prevention
2.
Prensa méd. argent ; 108(4): 194-200, 20220600. tab, graf
Article in Spanish | LILACS, BINACIS | ID: biblio-1381484

ABSTRACT

Introducción: La punción aspiración con aguja fina (PAAF) de tiroides se fundamentó y desarrolló por completo en 1950. Los informes citológicos eran descriptivos, no sistematizados, no comparables y rara vez indicaban el manejo del paciente. En 2007, en el Instituto Nacional del Cáncer de los Estados Unidos en Bethesda, se realiza el manual de la Nomenclatura y Sistemática de elaboración de los informes de PAAF tiroidea (Sistema Bethesda). En la actualidad, es una herramienta diagnóstica imprescindible para la evaluación de nódulos tiroideos y útil para decidir conducta e indicar la intervención quirúrgica. Objetivos: determinar el valor de la citología por PAAF en el diagnóstico final de los tumores tiroideos y comparar con el estudio patológico intraoperatorio y definitivo. Diseño: Estudio retrospectivo observacional. Lugar de aplicación: Hospital Público de atención terciaria de oncología. Materiales y métodos: se evaluaron todos los pacientes operados por patología tiroidea de enero de 2018 a diciembre de 2020, con PAAF previa y estudio patológico intraoperatorio. Resultados: La PAAF tuvo una sensibilidad de 96% y una especificidad del 70% con 1 falso negativo (FN) y 6 falsos positivos (FP), un valor predictivo positivo (VPP) de 85% y un valor predictivo negativo (VPN) de 87%. El porcentaje de malignidad según categoría de Bethesda fue: 28% para la categoría IV, 91% para V y 100% para VI. El estudio patológico intraoperatorio (EPI) tuvo una sensibilidad de 97% y especificidad del 83,3%, con 1 FN y 1 FP, un VPP de 96% y un VPN de 86%. Conclusión: El estudio de los tumores de tiroides por medio de la PAAF y su informe mediante el Sistema de Bethesda demostró, en general, ser confiable para evaluar el riesgo de malignidad tiroidea y la adecuada indicación de tratamiento quirúrgico a los pacientes estudiados en el preoperatorio.


Background: Thyroid fine needle aspiration (FNA) was fully founded and developed in 1950. Cytological reports were descriptive, not systematized, and not comparable and rarely indicated patient management. The manual of the Nomenclature and Systematics of preparation of the thyroid FNA reports (Bethesda System) was created in 2007, at the National Cancer Institute of the United States. At present, it is an essential diagnostic tool for the evaluation of thyroid nodules and useful for deciding on the conduct and indicating surgical intervention. Objectives: to determine the value of FNA cytology in the final diagnosis of thyroid tumors and to compare it with the intraoperative and definitive pathological study. Design: Retrospective observational study. Setting: Public Hospital for tertiary care of tumors. Material and methods: all patients surgically intervened for thyroid pathology from January 2018 to December 2020, with previous FNA and intraoperative pathological study, were evaluated. Results: FNA had a sensitivity of 96% and a specificity of 70% with one false negative (FN) and six of them with false positives (FP), a positive predictive value (PPV) of 85% and a negative predictive value (NPV) of 87%. The percentage of malignancy according to Bethesda category was: 28% for category IV, 91% for V and 100% for VI. Intraoperative histologic study (HIS) had a sensitivity of 97% and a specificity of 83.3%, with 1 FN and 1 FP, a PPV of 96% and a NPV of 86%. Conclusions: The study of thyroid tumors using FNA and its report using the Bethesda System proved, generally, to be reliable in evaluating the risk of thyroid malignancy and the adequate indication of surgical treatment in preoperative studied patients.


Subject(s)
Humans , Adult , Middle Aged , Aged , Retrospective Studies , Thyroid Nodule/surgery , Thyroid Nodule/diagnosis , Cell Biology , Biopsy, Fine-Needle/methods
3.
Rev. argent. cir ; 113(2): 205-215, jun. 2021. graf
Article in Spanish | LILACS-Express | LILACS, BINACIS | ID: biblio-1365475

ABSTRACT

RESUMEN Antecedentes: la cervicotomía de Kocher ha sido el abordaje clásico para la cirugía tiroidea y paratiroidea. El aumento en la preocupación por la cicatriz cervical ha generado un interés creciente por disminuir su tamaño o trasladarla a lugares menos visibles. Anuwong publica 60 casos de TOETVA/TOEPVA en 2016, con resultados óptimos. Objetivos: comunicar la experiencia con la tiroidectomía y paratiroidectomía transoral endoscópica con abordaje vestibular en el Hospital Universitario Austral. Material y métodos: análisis retrospetivo, sobre base prospectiva, de 18 procedimientos, entre mayo de 2019 y marzo de 2020. Se realizaron 2 paratiroidectomías, 13 lobectomías, 4 tiroidectomías totales, una con linfadenectomía central; una paciente presentó 2 patologías (adenoma paratiroideo y nódulo tiroideo). Citología según Bethesda: categoría II: 7; indeterminada: 1 y categoría VI: 9. Resultados: 18 pacientes femeninas. Mediana de edad: 41 años. Media del tamaño nodular: 30 mm. Volumen glandular medio: 24 mL. Conversión: 1 caso. Promedio de tiempo quirúrgico: lobectomía, 260 minutos; tiroidectomía total, 262 minutos. Histología definitiva: carcinoma papilar, 11; bocio nodular, 6; adenoma paratiroideo, 2. Complicaciones: equimosis leve, 12 pacientes; hipoparatiroidismo transitorio, 1 caso; paresia recurrencial transitoria, 1 caso; hiposensibilidad mentoniana transitoria, 1 caso. Dos casos de tiroidectomía total por cáncer: tiroglobulina a las 6 semanas < 0,1 μUI/mL. Conclusiones: 1) Es un abordaje seguro y ofrece resultados cosméticos excelentes. 2) Puede implementarse con equipamiento endoscópico convencional, con curva de aprendizaje corta y escasa morbilidad. 3) Alternativa para el tratamiento del carcinoma papilar de bajo riesgo. 4) Es prioritario garantizar la seguridad del paciente.


ABSTRACT Background: Kocher's cervicotomy has been the classic approach for thyroid and parathyroid surgery. The greater concern about neck scarring has generated an increasing interest in reducing scar size or leaving the scar in less visible places. In 2016 Anuwong published 60 cases of TOETVA/TOEPVA with optimal outcomes. Objectives: The aim of this study is to report the initial experience with transoral endoscopic thyroidectomy and parathyroidectomy vestibular approach in Hospital Universitario Austral. Material and methods: We conducted a retrospective analysis of 18 prospective procedures performed between May 2019 and March 2020. The procedures performed were 2 parathyroidectomies, 13 lobectomies, 4 total thyroidectomies, 1 with central lymph node dissection; one patient presented a parathyroid adenoma and a benign thyroid nodule. Cytology report according to the Bethesda system: category II in 7 cases, VI in 9 and indeterminate in 1. Results: 18 female patients. Median age: 41 years. Mean nodule size: 30 mm. Mean gland volume: 24 mL. Conversion. 1 case. Mean operative time: lobectomy, 260 minutes; total thyroidectomy, 262 minutes. Definite histology: papillary thyroid carcinoma,11; benign nodular goiter, 6; parathyroid adenoma, 2. Complications: mild ecchymosis, 12 patients; temporary hyperparathyroidism, 1 case; temporary recurrent laryngeal palsy, 1 case; temporary numbness of the mental region in 1 case. In the two cases undergoing total thyroidectomy due to cancer, thyroglobulin level 6 weeks after surgery was < 0.1 μIU/mL. Conclusions: 1) The transoral approach is a safe and feasible procedure that offers excellent cosmetic results. 2) It can be implemented using conventional endoscopic equipment, has a short learning curve and low morbidity rate. 3)It constitutes an alternative for the treatment of low-risk papillary thyroid carcinoma. 4) Patient's safety should be guaranteed.


Subject(s)
Humans , Male , Female , Thyroidectomy , Parathyroidectomy , Adenoma , Cicatrix , Cell Biology , Goiter, Nodular
4.
Rev. habanera cienc. méd ; 20(3): e4086, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1280431

ABSTRACT

Introducción: La citología vaginal directa es un método muy utilizado para la evaluación del ciclo estral de las ratas de laboratorio, pero la información acerca de los procedimientos e interpretación de los resultados aparece disgregada en la literatura, lo cual dificulta su empleo en los estudios de reproducción. Objetivo: Proponer un protocolo para la realización de la citología vaginal directa de ratas de laboratorio y la interpretación de los resultados. Material y métodos: Se combinó la información de la literatura y la experiencia de 10 años de estudios de reproducción, en los que se han empleado un total de 250 ratas Wistar hembras, siguiendo preceptos éticos establecidos. Se describen los procedimientos para la obtención de las muestras, mediante lavado vaginal, así como para su observación y análisis en estado húmedo, con microscopio óptico y cámara digital acoplada. Resultados: Se describen los tipos celulares principales presentes en el lavado vaginal y las características que permiten identificar cada fase o estado de transición del ciclo estral. Se discuten aspectos a considerar en la interpretación de los resultados, que incluye la relación con los cambios hormonales, los cuidados en la obtención de la muestra y la influencia de factores ambientales. Se muestran imágenes y figuras representativas para ilustrar el texto. Conclusiones: El trabajo constituye un protocolo para el estudio del ciclo estral de ratas de laboratorio, mediante la citología vaginal directa. Provee métodos no invasivos, sencillos y económicos, así como conocimientos esenciales para la interpretación de los resultados, que integran una guía de gran utilidad para los estudios experimentales de reproducción(AU)


Introduction: Direct vaginal cytology is a widely used method for the evaluation of the estrous cycle of laboratory rats, but the information about the procedures and interpretation of results is dispersed through literature, making its use difficult in investigations on reproduction. Objective: To propose a protocol for performing direct vaginal cytology of laboratory rats as well as for the interpretation of the results. Material and methods: The information obtained from literature and the experience of 10 years of investigation on reproduction in 250 female Wistar rats were combined following the established ethical principles. The procedures for obtaining samples by vaginal washing and by observation and analysis in humid state with light microscope equipped with digital camera were described. Results: The main types of cells present in the vaginal washing and the characteristics that allow us to identify each phase or transitional phases of the estrus cycle are described. Aspects to take into consideration in the interpretation of the results, which include the association between hormonal changes, the cares in the obtaining of the sample and the influence of environmental factors, are discussed. Representative images and figures are included to illustrate the text. Conclusions: The work consists of a study protocol of the estrus cycle of laboratory rats by direct vaginal cytology. It provides noninvasive, simple and cost-reducing procedures as well as essential knowledge for the interpretation of results which integrate a very useful guideline for experimental investigations on reproduction(AU)


Subject(s)
Rats , Rats, Wistar , Cell Biology , Estrous Cycle , Occupational Groups , Intervention Studies
5.
Rev. bras. anal. clin ; 53(1): 9-14, 20210330.
Article in Portuguese | LILACS | ID: biblio-1290893

ABSTRACT

Cervical cancer is the second neoplasm that most affects women in Brazil. It is a slow-progressing disease with well-defined stages. When diagnosed early, it has a high probability of cure, and it is essential to study methods that help in its diagnosis. The main factor associated with this neoplasm is infection with HPV, a virus that infects approximately 75% of sexually active people. Currently in Brazil the most used prevention method is the Pap smear test. With further studies on molecular biology and HPV, screening methods for cervical carcinoma have emerged, based on the detection of HPV DNA. Among these new methods is the double staining p16 / Ki-67, a technique developed aiming at a high specificity for detecting high-grade lesions. This is a bibliographic review work with the objective of discussing how biomolecular methods can be important tools for the early diagnosis of cervical cancer. The most used methods to diagnose HPV are cytopathological, colposcopic and histopathological exams, however these methods can present high variations in the detection of neoplastic lesions. With this study, we concluded the importance of using double staining p16 / Ki-67 for an accurate diagnosis in cases of cervical cancer.


O câncer de colo de útero (CCU) é a segunda neoplasia que mais acomete mulheres no Brasil. É uma doença de progressão lenta com estágios bem definidos. Quando diagnosticado precocemente tem grande probabilidade de cura, sendo indispensável o estudo sobre métodos que auxiliem no seu diagnóstico. O principal fator associado a essa neoplasia é a infecção pelo HPV, vírus que infecta aproximadamente 75% das pessoas sexualmente ativas. Atualmente, no Brasil, o método de prevenção mais utilizado é o exame citológico de Papanicolaou. Com maiores estudos sobre biologia molecular e HPV surgiram métodos de triagem do carcinoma cervical, baseados na detecção de DNA-HPV. Entre esses novos métodos está a coloração dupla p16/Ki-67, técnica desenvolvida visando uma alta especificidade para detecção de lesões de alto grau. Este é um trabalho de revisão bibliográfica com o objetivo de discutir como métodos biomoleculares podem ser ferramentas importantes para o diagnóstico precoce do câncer de colo de útero. Os métodos mais utilizados para diagnosticar o HPV são os exames citopatológicos, colposcópicos e histopatológicos, porém esses métodos podem apresentar altas variações na detecção de lesões neoplásicas. Com este estudo, concluímos a importância do uso da coloração dupla p16/Ki-67 para um diagnóstico preciso em casos de CCU.


Subject(s)
Uterine Cervical Neoplasms , Papillomavirus Infections , Cell Biology , Squamous Intraepithelial Lesions
6.
Acta méd. colomb ; 46(1): 27-33, ene.-mar. 2021. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1278152

ABSTRACT

Resumen Propósito: determinar la efectividad de detección de lesiones orales potencialmente malignas de cuatro métodos por medio de revisión sistemática tipo paraguas. Fuente de los datos: la búsqueda fue realizada con bases de datos Pubmed y EBSCOhost. Restricción de los años 2013-2018. Estrategia de búsqueda: (early detection of cáncer) AND (mouth neoplasms), (early detection of cáncer) AND (diagnostic techniques and procedures), (mouth neoplasms) AND (diagnostic techniques and procedures). Selección de los estudios: fueron seleccionadas a través de lectura crítica y la lista de chequeo del formato PRISMA, aquellas revisiones sistemáticas cuyo objetivo sea evaluar la efectividad de al menos uno de los métodos de detección de lesiones orales potencialmente malignas, seis estudios cumplieron con los criterios de inclusión. Extracción de datos: mediante la realización de lectura crítica de las revisiones sistemáticas seleccionadas previamente. Resultado de síntesis de los datos: se determina la efectividad a través de la comparación de las medias de porcentajes de sensibilidad y especificidad. Las medias de sensibilidad y especificidad de Vizilite fueron de 81.31 y 25.4%, de Microlux DL de 82.63 y 69.52%, de azul de toluidina de 82.07 y 66.27% y de citología exfoliativa de 76.77 y 80.87%, respectivamente. Conclusiones: los métodos de detección de lesiones orales premalignas evaluados en el presente estudio requieren mayor respaldo de evidencia científica para validar su efectividad. El método con mayor eficacia es el Microlux/DL, debido a sus altos niveles de sensibilidad y especificidad.


Abstract Objective: to determine the effectiveness of four methods for detecting potentially malignant oral lesions through an umbrella systematic review. Source of data: the search was performed in the PubMed and EBSCOhost databases. Years of search limited to 2013-2018. Search strategy: (early detection of cancer) AND (mouth neoplasms), (early detection of cancer) AND (diagnostic techniques and procedures), (mouth neoplasms) AND (diagnostic techniques and procedures). Study selection: using critical reading and the PRISMA checklist, systematic reviews with the objective of evaluating the effectiveness of at least one of the detection methods for potentially malignant oral lesions were selected; six studies met the inclusion criteria. Data extraction: through critical reading of the previously selected systematic reviews. Results of data synthesis: effectiveness was determined by comparing the mean percentages of sensitivity and specificity. Vizilite's sensitivity and specificity means were 81.31 and 25.4%, Microlux DL's were 82.63 and 69.52%, toluidine blue's were 82.07 and 66.27%, and exfoliative cytology's were 76.77 and 80.87%, respectively. Conclusions: the detection methods for pre-malignant oral lesions evaluated in this study need greater scientific evidence to validate their effectiveness. The method with the greatest effectiveness is Microlux/DL, due to its high levels of sensitivity and specificity.


Subject(s)
Mouth Neoplasms , Early Detection of Cancer , Sensitivity and Specificity , Diagnostic Techniques and Procedures , Cell Biology
7.
Med. lab ; 25(3): 565-567, 2021.
Article in Spanish | LILACS | ID: biblio-1343143

ABSTRACT

Los nódulos tiroideos siempre han sido considerados problemas comunes en la práctica clínica, y con el fin de estudiarlos se han buscado diferentes técnicas de aproximación y diagnóstico a lo largo de los años [1,2]. Esto ha contribuido al aumento en su incidencia, ya que a mayor número de estudios de imágenes realizados, aumenta la probabilidad de encontrarlos, puesto que tan solo el 50% son detectados por palpación al examen físico [3]. Es importante mencionar que a pesar de que solo el 5% de los nódulos tiroideos finalmente presentan un diagnóstico maligno y requieren manejo quirúrgico, hace algunos años se llevaban a cabo exámenes médicos diagnósticos y manejos quirúrgicos invasivos y exhaustivos que no eran prácticos, necesarios ni costo efectivos [4]. Fue solo hasta hace aproximadamente 40 años que se empezó a difundir el uso de la técnica diagnóstica desarrollada en 1950 por Söderstrom, Einhorn, Franzèn y Zajicek en el Hospital Radiumhelmet, de Estocolmo, Suecia, y considerada actualmente como la prueba de elección para la evaluación de nódulos tiroideos: la aspiración con aguja fina (ACAF), la cual se describe segura, precisa y rentable


Subject(s)
Humans , Thyroid Nodule , Cell Biology , Molecular Diagnostic Techniques
8.
Autops. Case Rep ; 11: e2021278, 2021. graf
Article in English | LILACS | ID: biblio-1249013

ABSTRACT

Peritoneal lymphomatosis (PL) is a rare presentation of extranodal precursor leukemia/lymphoma. The presentation is often non-specific, leading to delayed diagnosis and treatment. In this case, though the preliminary diagnosis was established on ascitic fluid cytology, the disease progressed rapidly, leading to demise before initiating chemotherapy. Immunophenotyping and molecular studies, performed later, established a diagnosis of de novo B-cell precursor leukemia/lymphoma with MYC, BCL2 rearrangements (Double-hit lymphoma). MYC, BCL2 rearrangements are rarely reported in precursor B-lymphoma/leukemia which carry dismal prognosis. In this report, we illustrate autopsy findings of PL in an elderly gentleman who presented with ascites for evaluation.


Subject(s)
Humans , Male , Aged , Peritoneal Neoplasms , Precursor B-Cell Lymphoblastic Leukemia-Lymphoma/pathology , Ascites , Autopsy , Genes, myc , Cell Biology
9.
Rev. medica electron ; 42(6): 2609-2620, nov.-dic. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1150041

ABSTRACT

RESUMEN Introducción: el cáncer de tiroides representa un 1% del total de todos los tipos de cáncer. Su incidencia parece aumentar un 4% cada año, y en la actualidad es el octavo cáncer más frecuente en mujeres. Se utilizan medios diagnósticos para definir que paciente operar y la estrategia terapéutica a seguir. Objetivo: evaluar el resultado de los medios diagnósticos en los pacientes operados de cáncer de tiroides. Materiales y métodos: se realizó un estudio descriptivo, retrospectivo y observacional que incluye todos los pacientes operados de cáncer tiroides en el servicio de Cirugía General del Hospital Universitario "Comandante Faustino Pérez Hernández", en el período desde enero de 1993 a diciembre del 2018. Se confeccionó una planilla pararecopilar la información para el estudio de la base de datos y los resultados se presentaron en tablas de frecuencia, números y porciento. Resultados: el cáncer de tiroides fue más frecuente en el grupo etario de 31 a 50 años, predomino el sexo femenino, la variedad histológica papilar fue la más frecuente, el ultrasonido y la citología con aguja fina aportan resultados favorables para definir la estirpe biológica de los tumores del tiroides, no comportándose de igual forma la biopsia por congelación. Conclusiones: el cáncer de tiroides es más frecuente en pacientes relativamente jóvenes, del sexo femenino y de variedad papilar. El ultrasonido y la citología con aguja fina aportan criterios beneficiosos para definir conducta terapéutica (AU).


SUMMARY Introduction: thyroid cancer represents 1% of the total of all kinds of cancer. Its incidence seems to increase 4% every year, and at the present time it is the eighth more frequent cancer in women. Diagnostic means are used to determine what patient undergoes a surgery and the therapeutic strategy to follow. Objective: to evaluate the results of the diagnostic means used in patients who undergo a thyroid cancer surgery. Materials and methods: a retrospective, descriptive, observational study was carried out including all patients who underwent a surgery of thyroid cancer in the service of General Surgery of the University Hospital ¨Comandante Faustino Perez Hernandez¨ in the period from January 1993 to December 2018. A form was elaborated to collect the information for the study of the database; the results were presented in charts of frequency, numbers and percentages. Results: the thyroid cancer was more frequent in the age group of 31 to 50 years; the female sex prevailed; the most frequent variety was the histological papillary one; ultrasound and fine needle cytology yield favourable results to define the biological stock of the thyroid cancers unlike the behaviour of the biopsy by freezing. Conclusions: thyroid cancer of the papillary variety is more frequent in relatively young, female patients. Ultrasound and fine needle cytology yield beneficial criteria to define the therapeutic behaviour (AU).


Subject(s)
Humans , Male , Female , Thyroid Neoplasms/diagnostic imaging , Ultrasonography/methods , Patients , Cell Biology/instrumentation , Thyroid Cancer, Papillary/diagnosis
10.
Oncol. (Guayaquil) ; 30(3): 204-214, Diciembre 30, 2020.
Article in Spanish | LILACS | ID: biblio-1145722

ABSTRACT

Introducción: La citología de nódulos tiroideos es una técnica que, evita procedimientos quirúrgicos innecesarios por lo que se lo ha determinado como primera línea dentro del algoritmo de diagnóstico, el objetivo del estudio fue determinar la sensibilidad y la especificidad de la citología y biopsia por congelación frente al estudio histopatológico en el diagnóstico de nódulos tiroideos en pacientes atendidos en Solca desde el año 2009 -2017. Métodos: Es un estudio de tipo observacional, retrospectivo y de correlación diagnóstica; los datos fueron obtenidos de las historias clínicas de pacientes intervenidos quirúrgicamente por nódulos tiroideos con biopsia por congelación, a quienes se les realizó previamente un estudio citológico en el Departamento de patología de SOLCA de la ciudad de Cuenca, Ecuador. El cálculo del tamaño de la muestra fue de 324 casos. Resultados:324 casos fueron incluidos. El 8.3% correspondió a hombres y el 91.7% a mujeres. La media de la edad fue 51.8 años; la gran mayoría provenían de la provincia Azuay con el 64.8%. En los estudios citológicos el 34.6% (112 casos)corresponden a lesiones inflamatorias benignas; el 11.1% [36 casos]a patologías malignas y 14.2% (46 casos)fueron insatisfactorios. En la biopsia por congelación el mayor porcentaje estuvo concentrado en enfermedades benignas con un 62.6% y 35.5% a lesiones malignas. Hubo 6 casos con el 1.9% en donde fue diferido el criterio diagnóstico. En el histopatológico definitivo el 60.2% (195 casos)fueron patologías benignas y el 39.8% (129 casos)fueron lesiones malignas. La sensibilidad de la PAAF frente a histopatológico es alta con un 91.79%, pero la especificidad es baja con un 51.94%. La sensibilidad y la especificidad de la biopsia por congelación frente a histopatológico es alta con un 98.97% y 90.70% respectivamente lo que le confiere una metodología óptima. Conclusiones: La PAAF de tiroides demuestra ser una metodología útil en el diagnóstico de nódulos, siempre y cuando sea realizada y observada por personal capacitado. La biopsia por congelación constituye una técnica con alta sensibilidad y especificidad que nos permite discriminar lesiones benignas de las malignas. Palabras claves: Nódulo tiroideo, Biopsia con Aguja, Servicio de Patología en Hospital, Oncología Médica, Agencias Voluntarias de Salud, Biología Celular, Biopsia con Aguja Fina


Introduction:Cytology of thyroid nodules is a technique that avoids unnecessary surgical procedures and has therefore been determined as the first line within the diagnostic algorithm.General Objective:To determine the sensitivity and specificity of cytology and freezing biopsy versus histopathological study in the diagnosis of thyroid nodules in patients treated in Solca since 2009 -2017. Methods:This is an observational, retrospective and diagnostic correlation study; the data were obtained from the clinical histories of patients surgically treated by thyroid nodules with freeze biopsy, who underwent a cytological study in the Department of pathology of the city of Cuenca, Ecuador. The calculation of the sample size was 324 cases. Results:8.3% corresponded to men and 91.7% to women. The mean age was 51.8 years; The vast majority came from the province of Azuay with 64.8%. In cytological studies, 34.6% [112 cases]correspond to benign inflammatory lesions; 11.1% [36 cases]to malignant pathologies and 14.2% [46 cases]were unsatisfactory. In the freeze biopsy the greater percentage was concentrated in benign diseases with 62.6% and 35.5% to malignant lesions. There were 6 cases with 1.9% where the diagnostic criterion was deferred. In the definitive histopathological, 60.2% [195 cases]were benign pathologies and 39.8% [129 cases]were malignant lesions. The sensitivity of FNAB to histopathological is high with 91.79%, but the specificity is low with 51.94%. The sensitivity and specificity of freezing versus histopathological biopsy is high with 98.97% and 90.70% respectively, which gives it an optimal methodology. Conclusions: Thyroid PAAF proves to be a useful methodology in the diagnosis of nodules, as long as it is performed and observed by trained personnel. Freezing biopsy is a technique with high sensitivity and specificity that allows us to discriminate benign from malignant lesions. Key words:Thyroid Nodule; Biopsy, Needle;Pathology Department, Hospital; Medical Oncology; Voluntary Health Agencies; Cell Biology; Biopsy, Fine-Needle


Subject(s)
Humans , Pathology Department, Hospital , Biopsy, Needle , Thyroid Nodule , Voluntary Health Agencies , Cell Biology , Biopsy, Fine-Needle , Medical Oncology
11.
Univ. salud ; 22(3): 231-237, set.-dic. 2020. tab
Article in Spanish | LILACS, COLNAL | ID: biblio-1139844

ABSTRACT

Resumen Introducción: Actualmente, el cáncer de cérvix sigue siendo una enfermedad importante por su incidencia y mortalidad. Aunque se conoce su etiología generada por la infección del virus del Papiloma Humano (PVH) y sus métodos de tamizaje como la citología convencional y el test de ADN-PVH, aún es necesario reconocer diversas características que se relacionan con la aparición de este tipo de cáncer. Objetivo: Describir las características citológicas previas al diagnóstico de cáncer cervical en mujeres de una institución de salud de la ciudad de Medellín. Materiales y métodos: Estudio descriptivo. Se tomaron los registros clínicos correspondientes a mujeres con diagnóstico confirmado de cáncer de cérvix entre 2012 y 2017 y se revisaron los resultados de citología previos al diagnóstico. Se realizó análisis univariado para describir las variables de estudio. Resultados: La muestra de mujeres con cáncer fue de 714, la mediana de edad fue 51 años (rango intercuartílico: 42-60), la alteración citológica más frecuente fue lesión intraepitelial de alto grado (33,8%), el principal cáncer diagnosticado fue carcinoma escamocelular (70,6%). Conclusiones: Los resultados de citología alteradas son un indicador para riesgo de cáncer de cérvix, de ahí la importancia de continuar con el protocolo de tamizaje establecido.


Abstract Introduction: Currently, cervical cancer continues to be an important disease because of its high incidence and mortality. Even though its etiology (Human Papilloma Virus infection) and screening methods (conventional cytology and HPV-DNA test) are well known, understanding the various characteristics associated with the appearance of cervical cancer is fundamental. Objective: To describe the cytological characteristics that precede the diagnosis of cervical cancer in women diagnosed in a health care institution from the city of Medellín. Materials and methods: A descriptive study. The clinical records corresponding to women with a confirmed diagnosis of cervical cancer between 2012 and 2017 were used. Also, the citology results obtained before the diagnosis were reviewed. An univariate analysis was carried out in oder to describe the study variables. Results: The sample size of women with cervical cancer was 714, the age median was 51 years old (interquartile range: 42-60), the most frequent cytological alteration was high-grade intraepithelial lesion (33.8%), and the main diagnosed cancer type was squamous cell carcinoma (70.6%). Conclusions: Results showing an altered cytology are indicators of cervical cancer risk. Therefore, it is important to continue with the stablished screening protocol.


Subject(s)
Cervix Uteri , Cell Biology , Uterine Cervical Neoplasms , Colposcopy , Squamous Intraepithelial Lesions of the Cervix
12.
Rev. med. Risaralda ; 26(1): 28-37, ene.-jun. 2020. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1127001

ABSTRACT

Resumen Introducción: La infección genital por el Virus de Papiloma Humano (VPH) se ha asociado con el cáncer cérvicouterino (CCE) al provocar la aparición de lesiones precursoras de cáncer en la zona de transformación de la unión escamo-columnar del cuello uterino. Existen más de 100 tipos de VPH, clasificados en bajo riesgo oncogénico (VPH-BR) y alto riesgo oncogénico (VPH-AR). Estudios reportan la infección por genotipos de alto riesgo en el 100% de los CCE. En Venezuela, el 67,7% de los CCE, se relacionan con el genotipo de VPH-AR 16. Objetivo: Detectar la presencia de VPH en pacientes con cambios citológicos cervicouterino. Metodología: Se incluyeron 49 pacientes que presentaban cambios citológicos, se tomaron las muestras de la región endocervical y exocervical para la detección y genotipificación del virus mediante la técnica de Multiple PCR. Resultados: Las alteraciones citológicas presentes fueron Células Escamosas Atípicas (69,4%), Células Glandulares Atípicas (4,1%), Lesión Escamosa Intraepitelial de Bajo Grado (16,3%), y Lesión Escamosa Intraepitelial de Alto Grado (10,2%). La detección molecular demostró que 16,3% presentaba VPH, 62,5% correspondían a VPH-AR, 25% a VPH-BR, 12,5% al genotipo 16 y no se detectó el genotipo 18. Se reportó un solo caso de coinfección. Conclusiones: A diferencia de otros estudios, no se encontró una relación estadísticamente significativa entre la presencia del virus y la aparición de cambios citológicos cervicouterino en esta población. No obstante, se detectaron genotipos de alto riesgo oncogénico, lo que puede traducirse en una mayor incidencia de cáncer cervicouterino a futuro.


Abstract Introduction: Genital infection by the Human Papilloma Virus (HPV) has been associated with cervical cancer (CC) since it causes the appearance of precursor cancer lesions in the transformation area of ​​the squamous-columnar junction of the cervix. There are more than 100 types of HPV that are classified as low oncogenic risk (LR-HPV) and high oncogenic risk (HR-HPV). Studies report that the infection by high-risk genotypes is present in 100% of CC. In Venezuela, 67.7% of CC is related to the HPV-16 genotype. Objective: This study seeks to detect the presence of HPV in patients with cervical cytological cell changes. Methodology : Forty-nine patients with cytological changes were studied. The endocervical and ectocervical areas were sampled to detect and genotype the virus by using the Multiplex PCR technique. Results: The cytological alterations presented were: Atypical Squamous Cells (69.4%), Atypical Glandular Cells (4.1%), Low-grade Squamous Intraepithelial Lesion (16.3%) and High-grade Squamous Intraepithelial Lesion (10.2%). Besides, the general molecular detection showed that 16.3% had HPV, 62.5% of it corresponded to HR-HPV, 25% to LR-HPV, and 12.5% ​​to genotype 16. The genotype 18 was not detected, and only one co-infection case was reported. Conclusions: Unlike other studies, a statistically significant relationship was not found between the virus presence and the appearance of cervical cytological cell changes in this population. However, genotypes with high oncogenic risk were detected, which may lead to a higher incidence of cervical cancer in the future.


Subject(s)
Humans , Female , Papillomaviridae , Uterine Cervical Neoplasms , Cervix Uteri , Polymerase Chain Reaction , Cell Biology , Reproductive Tract Infections , Atypical Squamous Cells of the Cervix , Gynecology , Venezuela , Human papillomavirus 16 , Coinfection , Multiplex Polymerase Chain Reaction , Squamous Intraepithelial Lesions , Genitalia , Herpes Zoster
13.
Infectio ; 24(1): 20-26, ene.-mar. 2020. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1090539

ABSTRACT

Resumen Objetivos del trabajo: Se determinaron los porcentajes de las lesiones precancerosas de cuello uterino en un grupo de mujeres diagnosticadas positivas por la prueba ADN-VPH. Materiales y métodos: El presente estudio es un análisis exploratorio descriptivo transversal de una base de datos con resultados de las pruebas de ADN-VPH (genotipo y tipo de infección), citología y colposcopia, realizadas en 58 mujeres de 30 años o más, para el periodo de octubre del 2018 a febrero del 2019. Resultados: De las 58 mujeres positivas para la prueba ADN-VPH, el 57% (n=33) fueron positivas para la prueba citológica cervical. De este grupo de mujeres fueron diagnosticadas negativas para LEI el 21% (n=7); para LEI-BG el 33% (n=11); y para LEI-AG el 45% (n=15) mediante la prueba de colposcopia. El VPH-16 mostró la mayor frecuencia relativa de detección en las LEI-AG con un 46,7% (n=7). Igualmente, los genotipos que cubre la vacuna Gardasil_4 fueron identificados en mayor porcentaje en las LEI-AG en comparación con los otros tipos histopatológicos diagnosticados, siendo esta asociación estadísticamente significativa, valor de p = 0,033. Conclusiones: La implementación de la nueva guía de práctica clínica para la detección y manejo de lesiones precancerosas de cuello uterino muestra resultados satisfactorios, siendo concordante la detección de ADN-VPH, con la identificación de anormalidades citológicas e histopatológicas, permitiendo la identificación precoz de mujeres en riesgo de desarrollar cáncer cervical.


Abstract Objectives of the study: To determine the percentages of precancerous lesions in the cervix in a group of women with positive diagnostic to the DNA-HPV test. Materials and methods: The present study is a cross-sectional exploratory analysis of a database with information on the results of DNA-HPV tests (genotype and type of infection), cytology and colposcopy, carried out on 58 women aged 30 or older, for the period from October 2018 to February 2019. Results: Of the 58 women positive for the DNA-HPV test, 57% (n=33) were positive for the cervical cytology test. Of this group of women, 21% (n=7) were diagnosed LEI-negative; for LEI-BG 33% (n=11); and for LEI-AG, 45% (n=15) using the colposcopy test. HPV-16 has a higher detection frequency in the LEI-AG with 46.7% (n=7). Likewise, the genotypes that cover the Gardasil_4 vaccine were members in a greater percentage in the LEI-AG in comparison with other diagnosed histopathological types, this association being statistically significant, value of p = 0.033. Conclusions: The implementation of the new clinical practice guideline for the detection and management of precancerous lesions of the cervix shows satisfactory results, the DNA-HPV detection being consistent, with the identification of cytological and histopathological abnormalities, allowing the early identification of women at risk to develop cervical cancer.


Subject(s)
Humans , Female , Adult , Precancerous Conditions , Uterine Cervical Neoplasms , Practice Guideline , Cervix Uteri , Colombia , Colposcopy , Cell Biology
14.
Rev. cienc. salud (Bogotá) ; 18(1): 37-51, ene.-mar. 2020. tab, graf
Article in English | LILACS, COLNAL | ID: biblio-1115528

ABSTRACT

Abstract Introduction: Cervical cancer is rare in young women, so screening in women under 21 years is not recommended. However, early and intense exposure to risk factors could increase the likelihood of early preneoplastic lesions. Currently, particular social conditions in adolescents can favor exposure, generate changes in lifestyles, and affect their immediate and future health. This work describes the frequency of risk factors and Pap results in adolescents of a child protection center. Materials and Methods: This paper presents a retrospective cross-sectional study that measured the frequency of exposure to risk factors for cervical cancer and Pap results of 889 adolescents exposed to critical social conditions. It used information from the cytology report files made between 2011 and 2016. Results: Normal Pap smear was found in 85 % of the cases. The risk factors had a very high prevalence, especially the early age of onset of sexual relations with 75 % before age 14, the number of sexual partners with more than one in 66.7 %, no use of a condom 95 % and high cigarette consumption. Discussion: The frequency of pre-malignant cervical lesions was very low; however, in women under 21 years old with premature and intense exposure to risk factors, an early-onset conservative screening accompanied by gynecological consultation for prevention, diagnosis, or treatment can be considered. The marginalized adolescent's social problem requires interdisciplinary and intersectoral management.


Resumen Introducción: el cáncer de cuello uterino es poco frecuente en mujeres jóvenes, por lo cual no se recomienda el tamizaje en menores de 21 años. Sin embargo, la exposición temprana e intensa a factores de riesgo podría aumentar la probabilidad de aparición de lesiones preneoplásicas tempranas. Ciertas condiciones sociales en las adolescentes pueden favorecer dicha exposición, generar cambios en los estilos de vida y afectar su salud inmediata y futura. Este trabajo describe la frecuencia de los factores de riesgo y los resultados de Papanicolaou en adolescentes de un centro de protección de menores. Materiales y Métodos: estudio retrospectivo de corte transversal que midió la frecuencia de exposición a factores de riesgo para el cáncer de cuello uterino y los resultados de Papanicolaou de 889 adolescentes marginadas. Utilizó información de los archivos de reporte de citologías realizadas entre 2011 y 2016. Resultados: se encontró Papanicolaou normal en el 85 %> de los casos. Los factores de riesgo tuvieron una muy alta prevalencia, especialmente la edad temprana de inicio de relaciones sexuales con el 75 %> antes de los 14 años, más de un compañero sexual (66.7 %>), no uso de preservativo 95 %> y alto consumo de cigarrillo. Discusión: la frecuencia de lesiones cervicales premalignas fue muy baja; sin embargo, en mujeres menores de 21 años con exposición prematura e intensa a factores de riesgo, podría considerarse un tamizaje conservador de inicio temprano, acompañado de consulta ginecológica para prevención, diagnóstico o tratamiento. El problema social de las adolescentes marginadas requiere un manejo interdisciplinario e intersectorial.


Resumo Introdução: o câncer de colo uterino é pouco frequente em mulheres jovens, pelo qual não se recomenda o rastreio em menores de 21 anos. No entanto, exposição precoce e intensa a fatores de risco poderia aumentar a probabilidade de aparição de lesões pré-neoplásicas precoces. Atualmente condições sociais particulares nas adolescentes podem favorecer a exposição, gerar mudanças nos estilos de vida e afetar sua saúde imediata e futura. Este trabalho descreve a frequência de fatores de risco e resultados de Papanicolau em adolescentes de um centro de proteção de menores. Materiais e Métodos: estudo retrospectivo de corte transversal que mediu a frequência de exposição a fatores de risco para o cancro de colo uterino e os resultados de Papanicolau de 889 adolescentes marginadas. Utilizou informação dos ficheiros de reporte de citologias realizadas entre 2011 e 2016. Resultados: se encontrou Papanicolau normal em 85 % dos casos. Os fatores de risco tiveram uma prevalência muito alta, especialmente em idade temporã de início de relações sexuais com um 75 % antes dos 14 anos, mais de um parceiros sexual no 66.7 %, não uso de preservativo 95 % e alto consumo de cigarro. Discussão: a frequência de lesões cervicais pré-malignas foi muito baixa, no entanto, em mulheres menores de 21 anos com exposição precoce e intensa a fatores de risco, se poderia considerar um rastreio conservador de início temporão acompanhado de consulta ginecológica para prevenção, diagnóstico ou tratamento. O problema social das adolescentes marginadas requere uma gestão interdisciplinar e intersetorial.


Subject(s)
Humans , Female , Child , Adolescent , Uterine Cervical Neoplasms , Social Medicine , Mass Screening , Risk Factors , Adolescent , Colombia , Cell Biology
15.
Int. j. morphol ; 38(1): 26-29, Feb. 2020. graf
Article in English | LILACS | ID: biblio-1090663

ABSTRACT

Mitochondria (m) are responsible for the energy availability of cells, and their analysis is indicated for example, in studies related to metabolism and oxidative stress. The direct measurement of mitochondria (morphometry) is biased because of the section obliquity and position relative to the mitochondria length (non-equatorial cut). Therefore, stereology is an appropriate technique to evaluate mitochondria. However, before beginning the study, it is necessary to consider the premises to obtain random and uniform samples to be analyzed stereology. Mitochondria must have the chance to appear in all the possibilities of cut and orientation in the micrographs. The number of micrographs to be analyzed will depend on the distribution and occupation of mitochondria in the cell. After this is resolved, a proposal is the estimation of the following stereological data: volume density (Vv), surface density (Sv), and mean cross-sectional area (A). Overlapping a known test area at each micrograph, the density by area of mitochondria is estimated (NAT). Vv [m] can easily be estimated by point-counting (Vv = Pp/PT; Pp are the points hitting the structure, PT are the number of points of the test system). Sv is estimated overlaying a test-line (LT) on the micrographs and counting the intersections of the lines (I) with the outer membrane (om), inner membrane (im), and crests (c), thus, Sv [om], Sv [im], Sv [c] (Sv = 2I / LT). A [m] is obtained as the ratio: A = Vv / 2NAT.


Las mitocondrias (m) son responsables de la disponibilidad de energía de las células, y su análisis está indicado, por ejemplo, en estudios relacionados con el metabolismo y el estrés oxidativo. La medición directa de las mitocondrias (morfometría) está sesgada debido a la oblicuidad de la sección y la posición relativa a la longitud de las mitocondrias (corte no ecuatorial). Por lo tanto, la estereología es una técnica apropiada para evaluar las mitocondrias. Sin embargo, antes de comenzar el estudio, es necesario considerar las premisas para obtener muestras aleatorias y uniformes para analizar estereológicamente. Es esencial que las mitocondrias tengan la posibilidad de aparecer en todas las posibilidades de corte y orientación en las micrografías. El número de micrografías que se analizarán dependerá de la distribución y ocupación de las mitocondrias en la célula. Una vez resuelto esto, una propuesta es la estimación de los siguientes datos estereológicos: densidad de volumen (Vv), densidad de superficie (Sv) y área de sección transversal media (A). Superponiendo un área de prueba conocida en cada micrografía, se estima la densidad por área de mitocondrias (NAT). Vv [m] se puede estimar fácilmente contando puntos (Vv = Pp / PT; Pp son los puntos que llegan a la estructura, PT son el número de puntos del sistema de prueba). Sv se estima superponiendo una línea de prueba (LT) en las micrografías y contando las intersecciones de las líneas (I) con la membrana externa (om), la membrana interna (im) y las crestas (c), por lo tanto, Sv [om], Sv [im], Sv [c] (Sv = 2I / LT). A [m] se obtiene como la relación: A = Vv / 2NAT.


Subject(s)
Microscopy, Electron, Transmission , Mitochondria/ultrastructure , Cell Biology
16.
Orient Journal of Medicine ; 32(1-2): 28-38, 2020. tab
Article in English | AIM | ID: biblio-1268294

ABSTRACT

Background:A majority of breast lesionisbenignin nature; benignbreast disease is four times more commonin Nigerian women. The percentage of unsatisfactory smears in breast cytology appears to behigher in benign conditions compared to malignant ones.The aim of this study is to determine the effectiveness of cytopathology in the diagnosis of benign breast disease in our institution.Methodology: This is a prospective study of 96 patients with benign breast disease seen during the study period. The patients were subjected to clinical assessment, fine needle aspiration cytology (FNAC) and open biopsy histopathology (as standardreference test).Results:One hundred andseventy-fourpatients with both FNACand histopathology reports were initially evaluated, 96 (55.2%) had benign while the rest (78, 44.8%) harbored malignant lumps. On analysis of the benign lumps, FNAC achieved high sensitivity (98.8%), specificity (96.9%) and overall diagnostic accuracy (98.0%) compared to clinical assessment with values of 83.3% (sensitivity), 82.1% (specificity) and 82.2% (overall diagnostic accuracy). The false positive rate (FPR, 2.3%) and false negative rate(FNR, 1.6%) reported for FNAC were equally better than figures of 14.9% (FPR) and 20.0% (FNR) documented for clinical assessment.Cytopathology was utilized insubclassifying 76 (79.2%) out of the 96 biopsy confirmed benign lumps; 49 slides were correctly typed giving a concordant rate of 64.5%.Conclusion:Fine needle aspiration cytology in our index study showed appreciable concordance with open biopsy histologyin the diagnosis and sub-classification of benign breast disease


Subject(s)
Biopsy , Cell Biology , Needles , Nigeria
17.
Rev. bras. ciênc. saúde ; 24(1): 17-28, 2020. tab
Article in Portuguese | LILACS | ID: biblio-1087470

ABSTRACT

Objetivo: Conhecer a tendência temporal de realização da citologia oncótica e identificar fatores associados à realização do exame em algum momento da vida e nos últimos três anos. Metodologia: Trata-se de um estudo epidemiológico, utilizando dados autorreferidos por mulheres, de 25 a 64 anos, oriundos do estudo transversal Vigitel. Para análise da tendência, dados de 2007 a 2015 da Capital João Pessoa, PB, foram analisados por meio de regressão linear simples e, para identificação dos fatores associados, razões de prevalências foram estimadas utilizando-se regressão de Poisson com dados de 2015. Resultados: A realização da citologia oncótica permaneceu, praticamente, estável no período de 2007 a 2015. Dentre as variáveis analisadas, a faixa etária manteve-se associada a uma maior prevalência de realização do exame nos dois desfechos e o estado civil casado/união estável manteve-se associado à maior prevalência de realização do exame em algum momento da vida; a escolaridade de 9 a 11 anos de estudo apresentou uma associação negativa com a realização do exame nos últimos três anos. Conclusão: A cobertura da citologia oncótica não foi satisfatória entre as mulheres de João Pessoa, Paraíba. A faixa etária mais avançada e as mulheres casadas ou em união estável apresentaram maior prevalência de realização do exame.(AU)


Objective: Know the time trend of performing cytology smears and identifying factors associated with performing the examination at some point in life and in the last three years. Methodology: It is an epidemiological study using self-reported data by women from 25 to 64 years old, from the cross-sectional Vigitel study. For the trend analysis, data from 2007 to 2015 in the city of João Pessoa, Paraiba, were analyzed by means of simple linear regression, and to identify associated factors, prevalence ratios were estimated using Poisson Regression, with data from 2015. Results: Oncotic cytology was practically stable from 2007 to 2015. Among the analyzed variables, age remained associated with a higher prevalence of performing the examination in both outcomes and the marital status married/in a stable union remained associated with a higher prevalence of performing the examination at any lifetime; education from 9 to 11 years of schooling showed a negative association with the completion of the exam in the last three years. Conclusion: The coverage of the Pap smear exam was not satisfactory among women in Joao Pessoa. The advanced age and married/in a stable union woman had higher prevalence of performing the examination. (AU)


Subject(s)
Humans , Female , Adult , Middle Aged , Uterine Cervical Neoplasms , Cell Biology , Papanicolaou Test , Neoplasms
18.
Rev. salud bosque ; 10(1): 1-11, 2020.
Article in Spanish | LILACS, COLNAL | ID: biblio-1104443

ABSTRACT

El fluido lagrimal se caracteriza por ser una mezcla de moléculas que incluye proteínas, lípidos, metabolitos, entre otros; estas moléculas juegan un papel importante en la fisiopatología de distintas enfermedades, por lo cual las lágrimas han sido de gran interés para la comunidad científica en la búsqueda de biomarcadores y en el desarrollo de estrategias terapéuticas para enfermedades sistémicas y oculares. La poca invasividad y el bajo riesgo al obtener la lágrima la convierten en una interesante muestra en comparación con algunos fluidos corporales que pueden ser mucho más costosos y molestos para su obtención. Lo anterior ha sido demostrado en diversos estudios que sugieren estrategias para obtener la muestra e indican un posterior análisis mediante avanzadas técnicas de biología molecular y celular, entre ellas los análisis ómicos, que han logrado una mejor caracterización lagrimal.Los análisis ómicos han contribuido en la identificación diferencial de distintas moléculas que pueden desempeñar un papel importante en el diagnóstico, seguimiento y/o tratamiento de enfermedades oculares y sistémicas. Por tanto, el propósito del presente artículo fue describir las diferentes características del fluido lagrimal, así como los posibles candidatos de biomarcadores de patologías oculares y sistémicas reportados.


The lacrimal fluid is characterized by a mixture of molecules from proteins, lipids, metabolites among others, which play an important role in the pathophysiology of different diseases, for which it has been of great interest in the scientific community for the search of biomarkers and development of therapeutic strategies in systemic and eye diseases. The lower invasiveness and risk when obtaining the tear, makes it an interesting sample compared to some bodily fluids that can be much more expensive and annoying to obtain. The above has been demonstrated through various studies in which they suggest different strategies to obtain the sample and its subsequent analysis using techniques of molecular and cellular biology among them advanced molecular technology such as those such as omic analyses, which have achieved a better lacrimal characterization. These analyses have contributed to the differential identification of different molecules that may play an important role in the diagnosis, monitoring and/or treatment of eye and systemic diseases. The purpose of this article is to describe the different characteristics of the tear fluid as well as the possible candidates for biomarkers of mainly reported eye and systemic pathologies.


O líquido lacrimal é caracterizado por ser uma mistura de moléculas que inclui proteínas, lipídios, metabólitos, entre outros; Essas moléculas desempenham um papel importante na fisiopatologia de diferentes doenças, motivo pelo qual as lágrimas têm sido de grande interesse para a comunidade científica na busca de biomarcadores e no desenvolvimento de estratégias terapêuticas para doenças sistêmicas e oculares. A baixa invasividade e o baixo risco de obter a lágrima fazem dela uma amostra interessante em comparação com alguns fluidos corporais que podem ser muito mais caros e problemáticos de obter. Isso foi demonstrado em vários estudos que sugerem estratégias para obter a amostra e indicam uma análise subsequente usando técnicas avançadas de biologia molecular e celular, incluindo análise ômica, que alcançaram melhor caracterização das lágrimas.A análise ômica contribuiu para a identificação diferencial de diferentes moléculas que podem desempenhar um papel importante no diagnóstico, monitoramento e / ou tratamento de doenças oculares e sistêmicas. Portanto, o objetivo deste artigo foi descrever as diferentes características do líquido lacrimal, bem como os possíveis candidatos a biomarcadores de patologias oculares e sistêmicas relatadas.


Subject(s)
Tears , Technology , Therapeutics , Methods , Cell Biology , Eye , Molecular Biology
19.
Article in English | WPRIM | ID: wpr-828960

ABSTRACT

Once pulp necrosis or apical periodontitis occurs on immature teeth, the weak root and open root apex are challenging to clinicians. Berberine (BBR) is a potential medicine for bone disorders, therefore, we proposed to apply BBR in root canals to enhance root repair in immature teeth. An in vivo model of immature teeth with apical periodontitis was established in rats, and root canals were filled with BBR, calcium hydroxide or sterilized saline for 3 weeks. The shape of the roots was analyzed by micro-computed tomography and histological staining. In vitro, BBR was introduced into stem cells from apical papilla (SCAPs). Osteogenic differentiation of stem cells from apical papilla was investigated by alkaline phosphatase activity, mineralization ability, and gene expression of osteogenic makers. The signaling pathway, which regulated the osteogenesis of SCAPs was evaluated by quantitative real time PCR, Western blot analysis, and immunofluorescence. In rats treated with BBR, more tissue was formed, with longer roots, thicker root walls, and smaller apex diameters. In addition, we found that BBR promoted SCAPs osteogenesis in a time-dependent and concentration-dependent manner. BBR induced the expression of β-catenin and enhanced β-catenin entering into the nucleus, to up-regulate more runt-related nuclear factor 2 downstream. BBR enhanced root repair in immature teeth with apical periodontitis by activating the canonical Wnt/β-catenin pathway in SCAPs.


Subject(s)
Animals , Berberine , Pharmacology , Cell Differentiation , Dental Papilla , Male , Osteogenesis , Periapical Periodontitis , Therapeutics , Rats , Stem Cells , Cell Biology , Metabolism , Wnt Signaling Pathway , Wnt3A Protein , Genetics , Metabolism , X-Ray Microtomography
20.
Article in English | WPRIM | ID: wpr-828956

ABSTRACT

A splicing mutation in VPS4B can cause dentin dysplasia type I (DD-I), a hereditary autosomal-dominant disorder characterized by rootless teeth, the etiology of which is genetically heterogeneous. In our study, dental follicle cells (DFCs) were isolated and cultured from a patient with DD-I and compared with those from an age-matched, healthy control. In a previous study, this DD-I patient was confirmed to have a loss-of-function splicing mutation in VPS4B (IVS7 + 46C > G). The results from this study showed that the isolated DFCs were vimentin-positive and CK14-negative, indicating that the isolated cells were derived from the mesenchyme. DFCs harboring the VPS4B mutation had a significantly higher proliferation rate from day 3 to day 8 than control DFCs, indicating that VPS4B is involved in cell proliferation. The cells were then replenished with osteogenic medium to investigate how the VPS4B mutation affected osteogenic differentiation. Induction of osteogenesis, detected by alizarin red and alkaline phosphatase staining in vitro, was decreased in the DFCs from the DD-I patient compared to the control DFCs. Furthermore, we also found that the VPS4B mutation in the DD-I patient downregulated the expression of osteoblast-related genes, such as ALP, BSP, OCN, RUNX2, and their encoded proteins. These outcomes confirmed that the DD-I-associated VPS4B mutation could decrease the capacity of DFCs to differentiate during the mineralization process and may also impair physiological root formation and bone remodeling. This might provide valuable insights and implications for exploring the pathological mechanisms underlying DD-I root development.


Subject(s)
ATPases Associated with Diverse Cellular Activities , Genetics , Case-Control Studies , Cell Differentiation , Genetics , Cells, Cultured , Dental Sac , Cell Biology , Dentin Dysplasia , Genetics , Pathology , Endosomal Sorting Complexes Required for Transport , Genetics , Humans , Mutation , Genetics , Osteogenesis , Genetics , RNA Splicing , Genetics
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