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1.
Clin. biomed. res ; 41(2): 117-125, 2021. tab, graf
Article in Portuguese | LILACS | ID: biblio-1293209

ABSTRACT

Introdução: Avaliar a associação entre níveis plasmáticos da quimiocina CCL11, coeficiente de inteligência e prática da amamentação em homens com esquizofrenia em condições psiquiátricas estáveis sob acompanhamento ambulatorial em um serviço de saúde pública. Métodos: Foi realizado estudo caso-controle com 60 indivíduos: 30 pacientes com esquizofrenia e 30 controles saudáveis, dos quais 15 de cada grupo foram expostos ao aleitamento materno e 15 não foram. Foi aplicado questionário abordando questões socioeconômicas, história ao nascer, dados clínicos e alimentação ao nascer. Foi dosada a quimiocina CCL11 e aplicados testes psicológicos para avaliar quociente de inteligência, funcionalidade, sintomas psiquiátricos, curso da doença e diagnóstico. Para os controles, foi utilizada uma escala para descartar doença psiquiátrica. Resultados: A quimiocina CCL11 apresentou valores significativamente mais altos (> 0,5) em pacientes com esquizofrenia quando comparados aos controles. No grupo de amamentados, os esquizofrênicos apresentaram valores significativamente mais altos a nível intermediário (entre 0.106 e 0.5). Não houve correlação da CCL11 com o número de hospitalizações, idade, tempo de diagnóstico e escolaridade. Não foi evidenciada correlação entre tempo de aleitamento materno em relação aos fatores do Brief Psychiatric Rating Scale. Houve uma tendência de correlação entre a idade de início da doença e o aleitamento materno. Foi encontrada correlação positiva do CCL11 com o tempo de aleitamento materno. Ao comparar os pacientes esquizofrênicos que foram aleitados com os que não foram, foi encontrada diferença estatisticamente significativa apenas para o quociente de inteligência. Conclusão: O aleitamento materno está associado a níveis mais baixos de CCL11, escores mais altos de quociente de inteligência e a esquizofrenia. A quimiocina CCL11 é mais alta em quem não amamentou, especialmente nos esquizofrênicos. (AU)


Introduction: To evaluate the association between plasma levels of chemokine CCL11, intelligence quotient, and exposure to breastfeeding in men with schizophrenia under stable psychiatric condition and monitored as outpatients in a public health care unit. Methods: A case-control study of 60 individuals, 30 patients with schizophrenia and 30 healthy controls; in each group, 15 were exposed to breastfeeding and 15 were not. A questionnaire addressing socioeconomic issues, history at birth, clinical data, and feeding at birth was administered. Chemokine CCL11 levels were measured, and psychological tests were applied to assess intelligence quotient, functional status, psychiatric symptoms, disease course, and diagnosis. A scale to rule psychiatric illness was used for the controls. Results: Chemokine CCL11 levels were significantly higher (> 0.5) in patients with schizophrenia than in controls. In the breastfed group, patients with schizophrenia also had significantly higher CCL11 levels, but at an intermediate level (between 0.106 and 0.5). There was no correlation between CCL11 and number of hospitalizations, age, time since diagnosis, or level of education, nor between duration of breastfeeding and the Brief Psychiatric Rating Scale factors. A trend toward a correlation was observed between age at disease onset and breastfeeding. There was a positive correlation between CCL11 and duration of breastfeeding. The comparison of patients with schizophrenia who were breastfed vs those who were not breastfed showed a statistically significant difference only in intelligence quotient. Conclusion: Breastfeeding is associated with lower CCL11 levels, higher intelligence quotient scores, and schizophrenia. Chemokine CCL11 levels are higher in those not exposed to breastfeeding, especially in patients with schizophrenia. (AU)


Subject(s)
Humans , Male , Adult , Middle Aged , Schizophrenia/epidemiology , Breast Feeding , Chemokine CCL11 , Intelligence/drug effects
2.
Braz. j. otorhinolaryngol. (Impr.) ; 83(4): 420-425, July-Aug. 2017. tab, graf
Article in English | LILACS | ID: biblio-889288

ABSTRACT

Abstract Introduction: In teenagers with perennial allergic rhinitis, exposure to tobacco cigarette smoke increases the count of eosinophils in the nasal mucosa; the recruitment of eosinophils arises from the combined action of a number of cellular and molecular signals, including eotaxin. Objective: To assess the effect of exposure to tobacco cigarette smoke on the count of immunoreactive cells to eotaxin-1 and eosinophils on the nasal mucosa of children and teenagers with perennial allergic rhinitis. Methods: In a cross-sectional study, forty-four patients were evaluated (aged 7-19 years old): 22 with and 22 with no exposure to tobacco cigarette smoke. After replying to 2 validated questionnaires, on Asthma and Allergies in Childhood and on the severity of nasal symptoms, nasal mucosal samples were obtained by scraping the middle one-third of the inferior turbinates. Then counts of immunoreactive cells to eotaxin-1 and eosinophils were assessed by immunohistochemistry. Results: Patients with exposure to tobacco cigarette smoke showed higher cell counts of both eotaxin-1 and eosinophils than patients with no exposure to the smoke, with no correlation between the two variables. However, both counts, of eotaxin-1 and eosinophils, were related to the cotinine/creatinine ratio. Conclusions: Exposure to tobacco cigarette smoke can increase eotaxin-1 and the count of eosinophils in the nasal mucosa of young patients with perennial allergic rhinitis.


Resumo Introdução: Em adolescentes com rinite alérgica perene, a exposição à fumaça do cigarro de tabaco aumenta a contagem de eosinófilos na mucosa nasal. O recrutamento de eosinófilos surge da ação combinada de alguns sinais celulares e moleculares, inclusive a eotaxina. Objetivo: Avaliar o efeito da exposição à fumaça do cigarro de tabaco na contagem de células imunorreativas a eotaxina-1 e eosinófilos na mucosa nasal de crianças e adolescentes com rinite alérgica perene. Método: Em um estudo transversal, 44 pacientes foram avaliados (entre sete e 19 anos): 22 com e 22 sem exposição à fumaça do cigarro de tabaco. Depois de responder a dois questionários validados, sobre asma e alergias na infância e sobre a gravidade dos sintomas nasais, as amostras de mucosa nasal foram obtidas por meio de raspagem do terço médio das conchas inferiores. Em seguida, as contagens de células imunorreativas para eotaxina-1 e eosinófilos foram avaliadas por imuno-histoquímica. Resultados: Os pacientes com exposição à fumaça do cigarro de tabaco apresentaram contagens de células mais elevadas tanto para eotaxina-1 como para eosinófilos em comparação com os pacientes sem exposição à fumaça, sem correlação entre as duas variáveis. No entanto, ambas as contagens, de eotaxina-1 e eosinófilos foram relacionadas com a razão cotinina/creatinina. Conclusões: A exposição à fumaça do cigarro de tabaco pode aumentar a eotaxina-1 e a contagem de eosinófilos na mucosa nasal de pacientes jovens com rinite alérgica perene.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Young Adult , Tobacco Smoke Pollution/adverse effects , Rhinitis, Allergic, Seasonal/immunology , Eosinophils/immunology , Chemokine CCL11/immunology , Nasal Mucosa/immunology , Severity of Illness Index , Immunohistochemistry , Cell Count , Rhinitis, Allergic, Seasonal/pathology , Cross-Sectional Studies , Eosinophils/cytology , Chemokine CCL11/analysis , Nasal Mucosa/cytology , Nasal Mucosa/chemistry
3.
Article in English | WPRIM | ID: wpr-49030

ABSTRACT

PURPOSE: Although the role of eosinophils in eosinophilic gastroenteritis (EGE) is not fully understood, they are believed to be a principal effector cell. Previous studies have demonstrated that eotaxin and its specific receptor, cysteine-cysteine chemokine receptor-3 (CCR3), play a central role in eosinophil trafficking into the gastrointestinal (GI) tract. Thus, we examined the targeting of CCR3 as a potential therapeutic intervention for EGE in a mouse model. METHODS: Eight- to 10-week-old BALB/c mice were intraperitoneally sensitized and intragastrically challenged with ovalbumin (OVA). Different groups of mice were administered either an anti-CCR3 antibody or a control IgG by intraperitoneal injection 1 hour before each OVA challenge. Eosinophilic inflammation in the intestinal mucosa, mucosal injury, and severity of diarrhea were compared between different groups at 1 hour after final OVA challenge. RESULTS: Anti-CCR3 antibody reduced the number of eosinophils in peripheral blood and intestinal mucosa, but not in bone marrow. This reduction was associated with restoration of reduced villous crypt ratio, increased intestinal epithelial cell proliferation, and weight loss induced by OVA challenge. However, Anti-CCR3 antibody had no effect on the level of OVA specific immunoglobulin E (IgE) and the expression of critical chemokines or cytokines in eosinophil trafficking into the GI tract, such as eotaxin-1, interleukin (IL)-5, and IL-13. CONCLUSIONS: Anti-CCR3 antibody significantly reduced the severity of eosinophilic inflammation, mucosal injury, and diarrhea in a mouse model of food allergen-induced GI eosinophilic inflammation. CCR3 may be a novel therapeutic target for treatment of EGE and other GI eosinophil-mediated diseases.


Subject(s)
Animals , Bone Marrow , Chemokine CCL11 , Chemokines , Cytokines , Diarrhea , Eosinophils , Epithelial Cells , Gastroenteritis , Gastrointestinal Tract , Immunoglobulin E , Immunoglobulin G , Immunoglobulins , Inflammation , Injections, Intraperitoneal , Interleukin-13 , Interleukins , Intestinal Mucosa , Mice , Ovalbumin , Ovum , Weight Loss
4.
Article in English | WPRIM | ID: wpr-27729

ABSTRACT

BACKGROUND/OBSECTIVE: Airway inflammation by eosinophils, neutrophils and alveolar macrophages is a characteristic feature of asthma that leads to pathological subepithelial thickening and remodeling. Our previous study showed that oxidative stress in airways resulted in eosinophilia and epithelial apoptosis. The current study investigated whether glutathione-containing dry yeast extract (dry-YE) ameliorated eosinophilia, goblet cell hyperplasia and mucus overproduction. MATERIALS/METHOD: This study employed 2 µg/mL lipopolysaccharide (LPS)- or 20 ng/mL eotaxin-1-exposed human bronchial epithelial cells and ovalbumin (OVA)-challenged mice. Dry-YE employed in this study contained a significant amount of glutathione (140 mg in 100 g dry yeast). RESULTS: Human bronchial epithelial cell eotaxin-1 and mucin 5AC (MUC5AC) were markedly induced by the endotoxin LPS, which was dose-dependently attenuated by nontoxic dry-YE at 10-50 µg/mL. Moreover, dry-YE inhibited the MUC5AC induction enhanced by eotaxin-1, indicating that eotaxin-1-mediated eosinophilia may prompt the MUC5AC induction. Oral supplementation with 10-100 mg/kg dry-YE inhibited inflammatory cell accumulation in airway subepithelial regions with a reduction of lung tissue level of intracellular adhesion molecule-1. In addition, ≥ 50 mg/kg dry-YE diminished the lung tissue levels of eotaxin-1, eosinophil major basic protein and MUC5AC in OVA-exposed mice. Alcian blue/periodic acid schiff staining revealed that the dry-YE supplementation inhibited goblet cell hyperplasia and mucus overproduction in the trachea and bronchiolar airways of OVA-challenged mice. CONCLUSIONS: Oxidative stress may be involved in the induction of eotaxin-1 and MUC5AC by endotoxin episode and OVA challenge. Dry-YE effectively ameliorated oxidative stress-responsive epithelial eosinophilia and mucus-secreting goblet cell hyperplasia in cellular and murine models of asthma.


Subject(s)
Animals , Apoptosis , Asthma , Chemokine CCL11 , Eosinophil Major Basic Protein , Eosinophilia , Eosinophils , Epithelial Cells , Glutathione , Goblet Cells , Humans , Hyperplasia , Inflammation , Lung , Macrophages, Alveolar , Mice , Mucin 5AC , Mucins , Mucus , Neutrophils , Ovalbumin , Ovum , Oxidative Stress , Trachea , Yeasts
5.
Article in Chinese | WPRIM | ID: wpr-286332

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of Wenyang Decoction (WD) on the differentiation of CD34+ progenitor cells of occupational asthma (OA) model rats.</p><p><b>METHODS</b>Fifty healthy male SD rats were randomly divided into five groups, i.e., the model group, the blank control group,the WD group,the Western medicine group,the combined group, 10 in each group. Prednisone suspension (10 mg/kg) was administered to rats in the Western medicine group by gastrogavage. WD (20 g/kg) was administered to rats in the WD group by gastrogavage. Prednisone suspension plus WD was administered to rats in the combined group by gastrogavage. Normal saline was administered to rats in the model group and the blank control group by gastrogavage. The general condition of rats was observed. Expression levels of peripheral blood IL-5 and eotaxin, eosinophils (EOS), CD34+, CC chemokine receptor 3 (CCR3+) in bone marrow suspension were detected by ELISA, Wirght-Giemsa, and flow cytometry, respectively.</p><p><b>RESULTS</b>Compared with the blank control group,expression levels of IL-5 and eotaxin in peripheral blood were significantly higher (P < 0.01), and the count of EOS and CD34+ cells, as well as CD34+ /CCR3+ significantly increased (P < 0.01) in the model group. Compared with the model group, expression levels of IL-5 and eotaxin, the count of EOS, CD34+ cells, CD34+ / CCR3+ were lowered in three treated groups (P < 0.01). Compared with the Western medicine group, the count of EOS and CD34+ / CCR3+ decreased in the combined group (P < 0.01). The count of EOS was significantly lower in the combined group than in the WD group (P < 0.01).</p><p><b>CONCLUSION</b>WD could reduce levels of in vivo inflammatory factors, and restrain the differentiation and recruitment of EOS,thereby alleviating the differentiation of CD34 progenitor cells to EOS.</p>


Subject(s)
Animals , Antigens, CD34 , Asthma, Occupational , Drug Therapy , Bone Marrow , Cell Differentiation , Chemokine CCL11 , Drugs, Chinese Herbal , Therapeutic Uses , Eosinophils , Flow Cytometry , Interleukin-5 , Male , Rats , Rats, Sprague-Dawley , Receptors, CCR3 , Stem Cells
6.
Article in Chinese | WPRIM | ID: wpr-746448

ABSTRACT

OBJECTIVE@#To explore the expression and significance of Eotaxin and RANTES in the rat model of allergic rhinitis (AR).@*METHOD@#20 female SD rats in 6-7 weeks were randomly divided into control group and AR group (n = 10, respectively). AR rat model was made with ovalbumin stimulation. To detect pathological changes in mucosa and chemokine Eotaxin, RANTES in their nasal and lung tissues after execution.@*RESULT@#Compared with the control group, Lung EOS cell counted higher in AR group and the difference was significant (P < 0.01); the AR rats nasal mucosa and lung tissue of Eotaxin, RANTES expression was significantly increased (P < 0.01).@*CONCLUSION@#There exist high expression of Eotaxin, RANTES, infiltration of eosinophils in nasal and lung tissue of model rats with allergic rhinitis, inferring that the upper and lower respiratory tract inflammatory response has obvious consistency.


Subject(s)
Animals , Chemokine CCL11 , Metabolism , Chemokine CCL5 , Metabolism , Disease Models, Animal , Female , Lung , Metabolism , Nasal Mucosa , Metabolism , Rats , Rats, Sprague-Dawley , Rhinitis, Allergic , Metabolism
7.
Article in Chinese | WPRIM | ID: wpr-747689

ABSTRACT

OBJECTIVE@#To investigate the distribution of mast cells in nasal polyps.@*METHOD@#Biopsy specimens from patients with nasal polyps (n = 20) and control patients (n = 8) were obtained and included in this study. The distribution of mast cells in nasal polyps and the expression of chemokines (CCL5, CCL11, CX3CL1, IL-8, IL-6) in the epithelial cells of normal nasal mucosa and nasal polyps was determined by immunohistochemistry.@*RESULT@#Mast cells migrate to intraepithelial in nasal polyps and the expression of chemokines (CCL5, CCL11, CX3CL1, IL-8) was up regulated in the epithelial cells of nasal polyps compare to normal nasal mucosa.@*CONCLUSION@#Our findings showed that mast cells migrate to intraepithelial in nasal polyps and the over expression of chemotaxins (CCL5, CCL11, CX3CL1, IL-8) may be response for mast cells' migration in nasal polyps. Mast cells might be associated with the development of nasal polyps.


Subject(s)
Chemokine CCL11 , Metabolism , Chemokine CCL5 , Metabolism , Chemokine CX3CL1 , Metabolism , Epithelial Cells , Metabolism , Humans , Immunohistochemistry , Interleukin-6 , Metabolism , Interleukin-8 , Metabolism , Mast Cells , Metabolism , Pathology , Nasal Mucosa , Cell Biology , Metabolism , Nasal Polyps , Metabolism , Pathology , Up-Regulation
8.
Scientific Journal of Kurdistan University of Medical Sciences. 2014; 19 (1): 67-77
in Persian | IMEMR | ID: emr-157559

ABSTRACT

Eotaxin, which is an important chemokine in asthma, was recently reported to be increased in obesity in mice and humans. The purpose of this study was to investigate the effect of exercise training on plasma eotaxin levels, insulin resistance, maximal oxygen consumption [Vo2max] and some of the major physiological factors in overweight and obese adolescents. In this experimental study, 28 healthy obese and overweight male adolescents were randomly selected. We divided the obese subjects into two groups: exercise group [N = 13] and control group [N=15]. The exercise group performed combined exercise training, 4 days a week [70 min/d] for 8 weeks. Vo2max, adiposity, insulin resistance, lipid profile and eotaxin were measured before and after the completion of exercise training. Independent T-test and Pearson's correlation coefficient were used for data analysis. p<0.05 was considered significant. The results showed that 8 weeks of interval combined exercise training increased eotaxine level and Vo2max, and decreased insulin resistance, percentage of body fat, weight, BMI, WC, and plasma levels of triglycerides in obese male adolescents[P<0.05]. There was no significant relationship between primary level of eotaxine with other factors [P<0.05]. In spite of increased eotaxine level after 8 weeks of interval combined exercise training, We observed positive effects on some of the physiological indices [TG, %BF, insulin resistance, central obesity, BMI and Vo2max] in overweight and obese adolescents


Subject(s)
Humans , Male , Asthma/prevention & control , Physical Education and Training , Insulin Resistance/physiology , Chemokine CCL11 , Overweight , Obesity , Oxygen Consumption
9.
Journal of Integrative Medicine ; (12): 195-205, 2013.
Article in English | WPRIM | ID: wpr-308253

ABSTRACT

<p><b>OBJECTIVE</b>Anti-asthma herbal medicine intervention (ASHMI(TM)), a combination of three traditional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic asthma, and a double-blind placebo-controlled clinical trial in patients with asthma. This study was designed to determine if the anti-inflammatory effects of individual herbal constituents of ASHMI(TM) exhibited synergy.</p><p><b>METHODS</b>Effects of ASHMI and its components aqueous extracts of Lingzhi (Ganoderma lucidum), Kushen (Sophora flavescens) and Gancao (Glycyrrhiza uralensis), on Th2 cytokine secretion by murine memory Th2 cells (D10.G4.1) and eotaxin-1 secretion by human lung fibroblast (HLF-1) cells were determined by measuring levels in culture supernatants by enzyme-linked immunosorbent assay. Potential synergistic effects were determined by computing interaction indices from concentration-effect curve parameters.</p><p><b>RESULTS</b>Individual Lingzhi, Kushen and Gancao extracts and ASHMI (the combination of individual extracts) inhibited production of interleukin (IL)-4 and IL-5 by murine memory Th2 cells and eotaxin-1 production by HLF-1 cells. The mean 25%-inhibitory-concentration (IC25) values (mg/mL) for ASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 30.9, 79.4, 123, and 64.6, respectively; for IL-5 production were 30.2, 263, 123.2 and 100, respectively; for eotaxin-1 were 13.2, 16.2, 30.2, and 25.1, respectively. The IC50 values (mg/mL) for ASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 158.5, 239.9, 446.7, and 281.8, respectively; for eotaxin-1 were 38.1, 33.1, 100, and 158.5, respectively. The interaction indices of ASHMI constituents at IC25 were 0.35 for IL-4, 0.21 for IL-5 and 0.59 for eotaxin-1. The interaction indices at IC50 values were 0.50 for IL-4 and 0.62 for eotaxin-1 inhibition. Inhibition of IL-5 did not reach IC50 values. All interaction indices were below 1 which indicated synergy.</p><p><b>CONCLUSION</b>By comparing the interaction index values, we find that constituents in ASHMI(TM) synergistically inhibited eotaxin-1 production as well as Th2 cytokine production.</p>


Subject(s)
Animals , Asthma , Drug Therapy , Metabolism , Cell Line , Chemokine CCL11 , Metabolism , Down-Regulation , Drug Synergism , Drugs, Chinese Herbal , Pharmacology , Fibroblasts , Metabolism , Humans , Interleukin-4 , Metabolism , Interleukin-5 , Genetics , Allergy and Immunology , Mice , Plants, Medicinal , Chemistry , Th2 Cells , Metabolism
10.
Article in Chinese | WPRIM | ID: wpr-747340

ABSTRACT

OBJECTIVE@#To investigate the relationship among the expressions of NF-kappaB, Eotaxin and the effects of tripterine in nasal mucosa of allergic rhinitis rat and to discuss the possible mechanism of tripterine on allergic rhinitis.@*METHOD@#Forty healthy SD rats were randomly divided into four groups: OVA group,tripterine group (T group), DM group, and SC group. Allergic rhinitis model was established by OVA. The pathological changes were observed by HE staining. The expressions of NF-kappaB and Eotaxin were examined by SP immunohistochemical analysis.@*RESULT@#There was no pathological change in SC group. Nasal mucosa in T and DM group was swelling,and there were some inflammatory cells. Nasal mucosa in OVA group was highly swelling, and there were abundant inflammatory cells. NF-kappaB and Eotaxin expression in OVA group was significantly different from the other three groups (P0.05). The expression of NF-kappaB in OVA group had positive correlation with the expression of Eotaxin (r=0.908, P<0.01).@*CONCLUSION@#Tripterine can inhibit expression of Eotaxin by restraining the activation of NF-kappaB.


Subject(s)
Animals , Chemokine CCL11 , Metabolism , NF-kappa B , Metabolism , Nasal Mucosa , Metabolism , Rats , Rats, Sprague-Dawley , Rhinitis, Allergic , Rhinitis, Allergic, Perennial , Metabolism , Triterpenes , Pharmacology
11.
Article in Chinese | WPRIM | ID: wpr-320643

ABSTRACT

<p><b>OBJECTIVE</b>Andrographolide, the active component in andrographis paniculata, has potent anti-inflammatory actions. This study aimed to evaluate the effects of andrographolide on eosinophil granulocytes (EOS) and the expression of eotaxin and IL-5 in mice with asthma.</p><p><b>METHODS</b>BALB/c mice were randomly assigned into normal control, asthma, budesonide treatment and andrographolide treatment groups (n=8 each). Mice in the latter three groups were sensitized and challenged with ovalbumin (OVA) to induce asthma. ELISA was used to detect the concentrations of eotaxin and IL-5 in bronchoalveolar lavage fluid (BALF) and peripheral blood. The expression of eotaxin mRNA and IL-5 mRNA in lung tissues was detected by real-time quantitative PCR.</p><p><b>RESULTS</b>Andrographolide treatment significantly decreased EOS count in BALF (P<0.05) and the effect of andrographolide was better than the effect of budesonide. Andrographolide treatment significantly down-regulated the expression of eotaxin and IL-5 in BALF, lung eotaxin mRNA expression and blood IL-5 expression (P<0.05), but the effects of andrographolide were poorer than the effects of budesonide. Andrographolide treatment resulted in a decrease in blood eotaxin expression and lung IL-5 mRNA expression and the effects of andrographolide were similar to budesonide.</p><p><b>CONCLUSIONS</b>Andrographolide can down-regulate the expression of IL-5 and eotaxin and thus suppress the inflitration of EOS in a mouse model of asthma.</p>


Subject(s)
Animals , Asthma , Drug Therapy , Bronchoalveolar Lavage Fluid , Cell Biology , Chemokine CCL11 , Genetics , Diterpenes , Pharmacology , Eosinophils , Physiology , Female , Interleukin-5 , Genetics , Mice , Mice, Inbred BALB C , RNA, Messenger
12.
Article in English | WPRIM | ID: wpr-182110

ABSTRACT

In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels of the Th2 cell-secreted cytokines, IL-4, IL-5, and IL-13 were markedly reduced in the PAR2 KO mice as compared to the wild type mice following infection with T. spiralis. The serum levels of parasite-specific IgE increased significantly in the wild type mice as the result of T. spiralis infection, but this level was not significantly increased in PAR2 KO mice. The expression level of thymic stromal lymphopoietin, IL-25, and eotaxin gene (the genes were recently known as Th2 response initiators) of mouse intestinal epithelial cells were increased as the result of treatment with T. spiralis excretory-secretory proteins. However, the expression of these chemokine genes was inhibited by protease inhibitor treatments. In conclusion, PAR2 might involve in Th2 responses against T. spiralis infection.


Subject(s)
Animals , Antibodies, Helminth/blood , Chemokine CCL11/biosynthesis , Cytokines/biosynthesis , Female , Gene Expression Profiling , Immunoglobulin E/blood , Interleukin-13/metabolism , Interleukin-4/metabolism , Interleukin-5/metabolism , Interleukins/biosynthesis , Mice , Mice, Inbred C57BL , Mice, Knockout , Receptor, PAR-2/metabolism , Th2 Cells/immunology , Trichinella spiralis/immunology , Trichinellosis/immunology
13.
Article in English | WPRIM | ID: wpr-134538

ABSTRACT

PURPOSE: Aspirin-intolerant asthma (AIA) is characterized by moderate to severe asthma that is aggravated by aspirin or other non-steroidal anti-inflammatory drugs. Affected patients frequently have chronic rhinosinusitis and nasal polyposis due to persistent upper and lower airway inflammation with marked eosinophilia. IL-13 plays a crucial role in the development of allergic asthma by inducing airway eosinophilia and hyper-reactivity and it has been correlated with an increased eosinophil count. METHODS: Two promoter polymorphisms of the IL-13 gene (-1510 A>C and -1055C>T) and one coding nonsynonymus Arg110Gln (110G>A) polymorphism were genotyped using primer extension methods in 162 patients with AIA, 301 patients with aspirin-tolerant asthma (ATA), and 430 normal healthy controls (NC). RESULTS: There was no significant difference in the genotype, allele, and haplotype frequencies of the three polymorphisms among the three groups. AIA patients with the AA genotype -1510A>C (P=0.012) and CC genotype -1055C>T (PA). CONCLUSIONS: These findings suggest that the IL-13 polymorphisms at -1510A>C and 1055C>T are associated with the development of rhinosinusitis in AIA patients. IL-13 Arg110Gln may be associated with an increased eosinophil count and eotaxin-1 level and could increase eosinophilic inflammation in the upper and lower airways of patients with AIA.


Subject(s)
Alleles , Aspirin , Asthma , Chemokine CCL11 , Clinical Coding , Eosinophilia , Eosinophils , Genotype , Haplotypes , Humans , Inflammation , Interleukin-13 , Polymorphism, Single Nucleotide
14.
Article in English | WPRIM | ID: wpr-134535

ABSTRACT

PURPOSE: Aspirin-intolerant asthma (AIA) is characterized by moderate to severe asthma that is aggravated by aspirin or other non-steroidal anti-inflammatory drugs. Affected patients frequently have chronic rhinosinusitis and nasal polyposis due to persistent upper and lower airway inflammation with marked eosinophilia. IL-13 plays a crucial role in the development of allergic asthma by inducing airway eosinophilia and hyper-reactivity and it has been correlated with an increased eosinophil count. METHODS: Two promoter polymorphisms of the IL-13 gene (-1510 A>C and -1055C>T) and one coding nonsynonymus Arg110Gln (110G>A) polymorphism were genotyped using primer extension methods in 162 patients with AIA, 301 patients with aspirin-tolerant asthma (ATA), and 430 normal healthy controls (NC). RESULTS: There was no significant difference in the genotype, allele, and haplotype frequencies of the three polymorphisms among the three groups. AIA patients with the AA genotype -1510A>C (P=0.012) and CC genotype -1055C>T (PA). CONCLUSIONS: These findings suggest that the IL-13 polymorphisms at -1510A>C and 1055C>T are associated with the development of rhinosinusitis in AIA patients. IL-13 Arg110Gln may be associated with an increased eosinophil count and eotaxin-1 level and could increase eosinophilic inflammation in the upper and lower airways of patients with AIA.


Subject(s)
Alleles , Aspirin , Asthma , Chemokine CCL11 , Clinical Coding , Eosinophilia , Eosinophils , Genotype , Haplotypes , Humans , Inflammation , Interleukin-13 , Polymorphism, Single Nucleotide
15.
Article in Chinese | WPRIM | ID: wpr-746666

ABSTRACT

OBJECTIVE@#To explore the expression of Eotaxin and the effect of histamine in allergic rhinitis model (AR), and aim to explore the pathogenesis of AR.@*METHOD@#The AR models were established by application of ovum albumin in rats. The expression of Eotaxin in nasal mucosa, serum and nasal cavity lavage fluid, were observed before and after treatment of histamine or its antagonist by immunochemistry, RT-PCR and ELISA technique.@*RESULT@#The expression of Eotaxin in nasal lavage fluid and nasal mucosa increased after treatment of histamine (P < 0.05). Contrarily, the expression of Eotaxin in nasal lavage fluid, nasal mucosa and serum decreased after treatment of the antagonist of histamine.@*CONCLUSION@#Both histamine and its receptor can involve in the pathogenesis of AR by affecting the expression of Eotaxin.


Subject(s)
Animals , Chemokine CCL11 , Metabolism , Female , Histamine , Metabolism , Male , Nasal Mucosa , Metabolism , Rats , Rats, Sprague-Dawley , Rhinitis, Allergic, Perennial , Metabolism , Pathology , Rhinitis, Allergic, Seasonal , Metabolism , Pathology
16.
Article in Chinese | WPRIM | ID: wpr-748261

ABSTRACT

OBJECTIVE@#To study the expression of Eotaxin and Eotaxin-2 in nasal polyp and observe the effects of steroids on Eotaxin and Eotaxin-2 in nasal polyps.@*METHOD@#The SP immunohistochemical method was applied to explore the expression of Eotaxin and Eotaxin-2 in nasal polyps before and after systemic corticosteroids therapy; the optical density of positive cells were measured by using HPIAL-2000 image-conduct system.@*RESULT@#The expression of Eotaxin and Eotaxin 2 were positive in mucosal epithelia, vascular endothelial, glandular epithelium, and inflammatory cells. After corticosteroids use, the number of eosinophils, the expression of Eotaxin in mucosal epithelia, inflammatory cells and vascular endothelial, and the expression of Eotaxin-2 in mucosal epithelia were significantly decreased (P<0.05). The steroids affected the expression of on Eotaxin-2 in mucosal epithelia of nasal polyps mostly.@*CONCLUSION@#1) The expression of Eotaxin and Eotaxin-2 in nasal polyp are positive. 2) The effects of steroid on the nasal polyps may depend on decreasing the infiltration of eosinophils and the expression of Eotaxin and Eotaxin-2.


Subject(s)
Adrenal Cortex Hormones , Pharmacology , Adult , Chemokine CCL11 , Metabolism , Chemokine CCL24 , Metabolism , Female , Humans , Male , Middle Aged , Nasal Mucosa , Metabolism , Nasal Polyps , Drug Therapy , Metabolism , Young Adult
17.
Article in Chinese | WPRIM | ID: wpr-814190

ABSTRACT

OBJECTIVE@#To investigate the expression of inflammatory cytokines in patients with recurrent nasal polyps by antibody chips.@*METHODS@#Proteins from the patients'nasal membrane in a nasal polyps group, a recurrent nasal polyps group, and a control group were labeled with biotin. The biotin-labeled proteins reacted with antibody chips on which the antibodies of 40 major inflammatory cytokines were prepared. The target proteins were conjugated with streptomycin antibody labeled with horseradish peroxidase (HRP),and signals were imaged by laser scanner.@*RESULTS@#Compared with the control group, the levels of inflammatory cytokines of nasal polyp group were notably increased, including pro-and anti-inflammatory cytokines, chemokines and certain cytokine receptors; while in recurrent nasal polyps, expression of chemokines were increased and most anti-inflammatory cytokines were decreased.@*CONCLUSION@#Antibody chips demonstrate a significant change in cytokine profiles in patients with recurrent nasal polypsis, as compared with those with nasal polyps. The abnormally higher expression of chemotatic factors in the nasal mucosa may play an important role in the recurrence of human nasal polyps.


Subject(s)
Adolescent , Adult , Aged , Antibodies , Chemokine CCL11 , Metabolism , Chemokines , Metabolism , Cytokines , Metabolism , Female , Humans , Interleukin-1beta , Metabolism , Interleukin-6 , Metabolism , Male , Middle Aged , Nasal Polyps , Metabolism , Protein Array Analysis , Methods , Recurrence , Young Adult
18.
Article in Chinese | WPRIM | ID: wpr-242364

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the therapeutic effect of Anti-anaphylaxis Granule (AAG) on chronic urticaria and its impact on cytokine of regulated upon activation of normal T cells expressed and secreted (RANTES), eosinophil chemotactic factor (Eotaxin) and tumor necrosis factor-alpha (TNF-alpha).</p><p><b>METHODS</b>The therapeutic effects of AAG and cetirizine on chronic urticaria patients allocated in two groups were observed respectively, and the serum levels of RANTES, Eotaxin and TNF-alpha in patients were measured by ELISA before and after treatment, and were compared with those in normal subjects.</p><p><b>RESULTS</b>The therapeutic effects of AAG group were better in effective rate (88.5% vs 64.0%) and lower in the recurrent rate (5.6% vs 41.9%) than those cetirizine (all P<0.05). Serum levels of RANTES, Eotaxin and TNF-alpha in patients were higher than those in normal subjects (P<0.01), and they could be significantly reduced after AAG treatment (P<0.01).</p><p><b>CONCLUSION</b>AAG has favorite effect for treatment of chronic urticaria, its regulation on serum levels of RANTES, Eotaxin and TNF-alpha may be the mechanism of action.</p>


Subject(s)
Adolescent , Adult , Cetirizine , Therapeutic Uses , Chemokine CCL11 , Blood , Chemokine CCL5 , Blood , Drugs, Chinese Herbal , Therapeutic Uses , Female , Humans , Male , Middle Aged , Phytotherapy , Serum , Metabolism , Tumor Necrosis Factor-alpha , Blood , Urticaria , Blood , Drug Therapy , Young Adult
19.
Journal of the Egyptian Society of Endocrinology, Metabolism and Diabetes [The]. 2008; 40 (1): 21-32
in English | IMEMR | ID: emr-99662

ABSTRACT

Hypoadiponectinemia is associated with insulin resistance and predicts the incidence of type 2 diabetes and coronary artery disease. Chronic subclinical inflammation and activation of innate immunity are involved in the pathogenesis of type 2 diabetes. Since obesity is associated with hypoadiponectinemia and with increased circulating levels of various immunological markers, which are both major risk factors for the development of type 2 diabetes, so the aim of this study was to investigate the association of hypoadiponectinemia and low grade systemic inflammation in type 2 diabetic patients as well as subjects with impaired glucose tolerance. Sixty male age matched subjects were included in the study. They were divided into 3 groups each of twenty as follows: newly diagnosed patients with type 2 diabetes mellitus [group I], patients with impaired glucose tolerance [group II] and healthy subjects with normal glucose tolerance [group Ill] as a control group. Patients and controls were subjected to full history taking, clinical examination stressing on blood pressure, BMI and WHR; laboratory investigations including FPG, PPG, HbA1C, fasting insulin and HOMA-IR index, lipid profile [TG, total cholesterol, HDL-C, LDL-C], serum uric acid, serum adiponectin and some immunological markers including WBC, acute phase reactants [CRP], TN F-alpha and eotaxin. In type 2 diabetic patients, plasma adiponectin levels were strongly negatively correlated with CRP, fasting TG, fasting insulin, HOMA-IR and fasting glucose [P < 0.001] and strongly positively correlated with HDL-cholesterol [P < 0.001]. Inverse correlations were found between adiponectin levels and WHR, postprandial glucose, and TNF-alpha [P < 0.05]. No significant correlation was found between adiponectin level and eotaxin [P > 0.05]. In subjects with IGT, an inverse relation was found between adiponectin and fasting glucose [P < 0.05]. The mean values of immunological markers [eotaxin, TNF-alpha, CRP and WBC] were significantly higher in type 2 diabetics versus control group. Subjects with IGT showed significant lower levels of eotaxin and TNF-alpha than diabetic patients, while they showed significant higher levels of eotaxin, TN F-alpha and CRP than controls [P < 0.05]. The mean value of adiponectin was significantly lower in type 2 diabetic patients than in subjects with IGT and the control group [P < 0.05]. The studied clinical and anthropometric parameters, lipid profile, parameters of glycemic control, fasting insulin and HOMA-IR were significantly higher in group I versus group II and the control group [P < 0.05]. Our results support the hypothesis that hypoadiponectinemia may be associated with low grade inflammation, metabolic abnormalities and dyslipidemia. Therefore, adiponectin may be an important link between inflammation and type 2 diabetes as it was negatively correlated with markers of inflammation in such patients


Subject(s)
Humans , Male , Chemokine CCL11/blood , Adiponectin/blood , Insulin Resistance , Tumor Necrosis Factor-alpha/blood , C-Reactive Protein , Anthropometry/methods , Body Mass Index , Waist-Hip Ratio/methods , Metabolism , Leukocyte Count
20.
Article in Chinese | WPRIM | ID: wpr-748894

ABSTRACT

OBJECTIVE@#To study the expression of Eotaxin and Eotaxin-2 in nasal polyposis and nasal polyp and explore the different mechanism between polyposis and polyp.@*METHOD@#Fifteen cases of nasal polyposis, thirteen cases of polyp and eight cases of normal middle turbinate were studied with immunohistochemical SP method to detect the expression of Eotaxin and Eotaxin-2.@*RESULT@#There were significant differences between either two groups about the expression of Eotaxin-2 (P 0.05).@*CONCLUSION@#Both of Eotaxin and Eotaxin-2 likely play key roles in the inflammatory reaction process of nasal polyposis and polyp. The different expression of Eotaxin-2 between nasal polyposis and polyp demonstrates it may be one of the main causes in the different mechanism of the two diseases.


Subject(s)
Adolescent , Adult , Chemokine CCL11 , Metabolism , Chemokine CCL24 , Metabolism , Child , Female , Humans , Male , Middle Aged , Nasal Polyps , Metabolism , Pathology , Young Adult
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