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J. appl. oral sci ; 28: e20190039, 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1090769


Abstract This study aimed to evaluate the physical properties and antifungal activities of polymethyl methacrylate (PMMA) acrylic resins after the incorporation of chlorhexidine diacetate salt (CDA). Methodology: First, acrylic resin specimens were fabricated with Vipi Cor® and DuraLay® resins with and without the incorporation of 0.5%, 1.0% or 2.0% CDA. The residual monomer and CDA release were measured at intervals ranging from 2 hours to 28 days using ultraviolet spectrometry combined with high-performance liquid chromatography. The antifungal activity against C. albicans was evaluated with the agar diffusion method. Fourier transform infrared spectroscopy was used to analyze the degree of resin conversion. Finally, the water sorption values of the resins were also measured. Results: The incorporated CDA concentration significantly changed the rate of CDA release (p<0.0001); however, the brand of the material appeared to have no significant influence on drug release. Subsequently, the inhibition zones were compared between the tested groups and within the same brand, and only the comparisons between the CDA 2% and CDA 1% groups and between the CDA 1% and CDA 0.5% groups failed to yield significant differences. Regarding the degrees of conversion, the differences were not significant and were lower only in the CDA 2% groups. Water sorption was significantly increased at the 1.0% and 2.0% concentrations. Conclusions: We concluded that the incorporation of CDA into PMMA-based resins enabled the inhibition of C. albicans growth rate, did not alter the degrees of conversion of the tested resins and did not change the release of residual monomers.

Chlorhexidine/analogs & derivatives , Polymethyl Methacrylate/chemistry , Antifungal Agents/chemistry , Reference Values , Time Factors , Acrylic Resins/chemistry , Materials Testing , Candida albicans/drug effects , Water/chemistry , Chlorhexidine/pharmacology , Reproducibility of Results , Chromatography, High Pressure Liquid , Spectroscopy, Fourier Transform Infrared , Polymethyl Methacrylate/pharmacology , Antifungal Agents/pharmacology
Braz. oral res. (Online) ; 33: e035, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001616


Abstract: Eugenia dysenterica is a Brazilian tree investigated for its properties and bioactive compounds, which are believed to have both pharmacological and phytochemical therapeutic effects. The leaves of this tree contain tannins, flavonoids, terpenes, and saponins, with reportedly beneficial effects to the human body. Despite these therapeutic applications, its effects have never been tested on oral tissues. Therefore, the aim of the present study was to evaluate the cytotoxic and antioxidant effects and the anti-inflammatory and repair properties of the acetone fraction of E. dysenterica on primary culture of human gingival fibroblasts and on the immortalized murine macrophage cell line (RAW 264.7). For this purpose, a metabolic activity assay, a wound healing assay, a nitric oxide assay, and RT-qPCR were performed. The assays revealed a cytoprotective effect of this plant, suggested by the increase in the expression of SOD1 and NRF2. An antioxidant potential effect was observed in the DPPH• assay. However, the fraction of E. dysenterica did not show anti-inflammatory activity. In conclusion, Eugenia dysenterica may promote cytoprotection when associated with chlorhexidine digluconate because of its antioxidant effect. However, additional studies are necessary on other human dental tissues using other parts of the plant in order to develop a possible mouthwash to assist patients with oral disorders.

Humans , Animals , Mice , Plant Extracts/pharmacology , Fibroblasts/drug effects , Eugenia/chemistry , Gingiva/chemistry , Anti-Inflammatory Agents/pharmacology , Antioxidants/pharmacology , Reference Values , Time Factors , Wound Healing/drug effects , Brazil , Cells, Cultured , Chlorhexidine/analogs & derivatives , Chlorhexidine/pharmacology , Reproducibility of Results , Plant Leaves/chemistry , Reverse Transcriptase Polymerase Chain Reaction , RAW 264.7 Cells , Nitric Oxide/analysis
Braz. oral res. (Online) ; 31: e89, 2017. tab, graf
Article in English | LILACS | ID: biblio-952126


Abstract The objective of this study was to evaluate the effects of Cymbopogon citratus essential oil and its main compound (citral) against primary dental colonizers and caries-related species. Chemical characterization of the essential oil was performed by gas chromatography/mass spectroscopy (GC/MS), and the main compound was determined. Antimicrobial activity was tested against Actinomyces naeslundii, Lactobacillus acidophilus, S. gordonii, S. mitis, S. mutans, S. sanguinis and S. sobrinus. Minimum inhibitory and bactericide concentrations were determined by broth microdilution assay for streptococci and lactobacilli reference, and for clinical strains. The effect of the essential oil on bacterial adhesion and biofilm formation/disruption was investigated. Negative (without treatment) and positive controls (chlorhexidine) were used. The effect of citral on preformed biofilm was also tested using the same methodology. Monospecies and microcosm biofilms were tested. ANOVA or Kruskal-Wallis tests were used (α=0.05). Cytotoxicity of the essential oil to human keratinocytes was performed by MTT assay. GC/MS demonstrated one major component (citral). The essential oil showed an inhibitory effect on all tested bacterial species, including S. mutans and L. acidophilus. Essential oil of C. citratus (10X MIC) reduced the number of viable cells of lactobacilli and streptococci biofilms (p < 0.05). The essential oil inhibited adhesion of caries-related polymicrobial biofilm to dental enamel (p < 0.01). Citral significantly reduced the number of viable cells of streptococci biofilm (p < 0.001). The essential oil showed low cytotoxicity to human keratinocytes. Based on these findings, this study can contribute to the development of new formulations for products like mouthwash, against dental biofilms.

Humans , Oils, Volatile/pharmacology , Biofilms/drug effects , Cymbopogon/chemistry , Dental Caries/microbiology , Dental Caries/prevention & control , Anti-Infective Agents/pharmacology , Reference Values , Streptococcus/growth & development , Streptococcus/drug effects , Time Factors , Bacterial Adhesion/drug effects , Actinomyces/growth & development , Actinomyces/drug effects , Colony Count, Microbial , Microbial Sensitivity Tests , Keratinocytes/drug effects , Cell Survival/drug effects , Chlorhexidine/analogs & derivatives , Chlorhexidine/pharmacology , Reproducibility of Results , Analysis of Variance , Statistics, Nonparametric , Dental Enamel/drug effects , Dental Enamel/microbiology , Lactobacillus acidophilus/growth & development , Lactobacillus acidophilus/drug effects , Gas Chromatography-Mass Spectrometry , Anti-Infective Agents, Local/pharmacology
Braz. dent. j ; 27(6): 646-651, Nov.-Dec. 2016. tab, graf
Article in Portuguese | LILACS | ID: biblio-828063


Resumo O objetivo do presente estudo foi avaliar clinicamente em humanos o efeito antiplaca de Ocimum gratissimum (Og). Quinze adultos saudáveis participaram deste estudo cruzado, duplo-cego, por meio de um modelo de acúmulo de placa parcial de 3 dias. Os voluntários aboliram qualquer método mecânico de higiene oral e foram inicialmente designados para usar os seguintes enxaguatórios bucais: água destilada (solução AD), digluconato de clorexidina a 0,12% (solução CLX) ou 10% Og (solução Og). O índice de placa (IPL) foi registrado em todos os dentes inferiores no final do experimento e os testes estatísticos Kruskal-Wallis (α=0,05) e Mann-Whitney (α=0,05) foram utilizados para estimar a diferença entre os grupos. Os resultados clínicos demonstraram diferença estatisticamente significante entre os três grupos (p<0,05), favorecendo os grupos CLX e Og; entretanto o grupo CLX foi mais efetivo que o grupo Og (p<0,05). Os enxaguatórios bucais contendo digluconato de clorexidina e Og a 10% foram capazes de reduzir a formação de nova placa bacteriana, mas Og mostrou resultados mais limitados em comparação ao digluconato de clorexidina.

Abstract The aim of this study was to evaluate the antiplaque effect of Ocimum gratissimum (Og) by in vivo investigation. Fifteen healthy volunteers participated in a crossover, double-blind clinical study, using a 3-day partial-mouth plaque accumulation model. The participants abolished any method of mechanical oral hygiene and they were randomly assigned to initially use just the following mouthrinses: distilled water (DW solution), 0.12% chlorhexidine digluconate (CLX solution) or 10% Og (Og solution). The plaque index (PLI) was recorded in all mandibular teeth at the end of the trial and the Kruskal-Wallis (α=0.05) and Mann-Whitney (α=0.05) tests were used to estimate the difference among groups. The clinical results showed statistically significant difference among the groups (p<0.05), favoring the CLX solution and Og solution, but the first was more effective (p<0.05). The mouthrinses containing 0.12% chlorhexidine digluconate and 10% Og were able to inhibit plaque re-growth, however Og showed more limited results in comparison to CLX.

Humans , Male , Female , Adolescent , Adult , Young Adult , Dental Plaque/prevention & control , Mouthwashes , Ocimum , Chlorhexidine/administration & dosage , Chlorhexidine/analogs & derivatives , Cross-Over Studies , Dental Plaque Index , Double-Blind Method
Braz. oral res. (Online) ; 30(1): e13, 2016. tab, graf
Article in English | LILACS | ID: lil-768259


Abstract The aim of this study was to evaluate the effect of green tea as a protective measure on eroded dentin. Disks of human coronary dentin were selected based on surface hardness and randomly assigned to 3 groups (n = 10): DW - distilled water, CHX - 0.2% chlorhexidine digluconate, and GT - green tea. The disks were allowed to acquire pellicle for 2 hours and were then subjected to 3 cycles per day of demineralization (C6H8O7 0.05 M, pH 3.75, 60 s), treatment (DW or CHX or GT, 5 min) and remineralization (artificial saliva, 60 min) over a period of 3 days. Changes in the dentin were determined by loss of surface hardness (%SHL) and mechanical profilometry analysis at the end of each day. Data were analyzed by two-way ANOVA followed by Tukey’s test for %SHL and profilometry (p < 0.05). Significant reductions in dentin hardness loss were observed only for the CHX group when compared to the DW group (p < 0.05). However, there was no significant difference between the CHX and GT groups (p > 0.05). A significant difference was observed between DW and GT treatments for wear and roughness measurements (p < 0.05). The green tea extract solution was able to reduce the wear and roughness caused by dentin erosion under the conditions of this study.

Humans , Dentin/drug effects , Protective Agents/chemistry , Tea/chemistry , Tooth Erosion/prevention & control , Analysis of Variance , Chlorhexidine/analogs & derivatives , Chlorhexidine/chemistry , Hardness , Matrix Metalloproteinases/chemistry , Plant Extracts/chemistry , Random Allocation , Reproducibility of Results , Saliva, Artificial/chemistry , Statistics, Nonparametric , Surface Properties/drug effects , Time Factors , Water/chemistry
Journal of Lasers in Medical Sciences. 2016; 7 (1): 21-25
in English | IMEMR | ID: emr-178965


Introduction: Considering the importance of prevention in periodontal diseases and the important role of Aggregatibacter actinomycetemcomitans in induction and progression of these diseases, the aim of the present in vitro study was to compare the antimicrobial effects of chlorhexidine digluconate [CHX], curcumin and light-emitting diode [LED] laser, on this bacterium

Methods: Antimicrobial activity of curcumin [5 mg/ml], CHX [2%], LED [120 J/cm[2]] and LED + curcumin [120 J/cm[2] + 2.5 mg/ml] against A. actinomycetemcomitans were tested in vitro, using micro-broth dilution test. One-way analysis of variance [ANOVA] and Tukey's HSD tests served for statistical analysis

Results: Regarding the minimum inhibitory concentration [MIC], CHX had a significantly lower MIC than curcumin [P<0.05]. Sorted out by bacterial growth from lowest to highest, were CHX, LED + curcumin, curcumin, and LED groups. All the differences were found to be statistically significant [P<0.05] except for the LED group

Conclusion: We conclude that curcumin is an effective substance in preventing the growth of A. actinomycetemcomitans, whose impact is reinforced when used simultaneously with photodynamic therapy [PDT]

In Vitro Techniques , Curcumin , Lasers, Semiconductor , Anti-Infective Agents , Photochemotherapy , Chlorhexidine/analogs & derivatives
Braz. oral res. (Online) ; 30(1): e125, 2016. graf
Article in English | LILACS | ID: biblio-951959


Abstract The aim of this study was to assess the in vitro antimicrobial effects of chlorhexidine digluconate (CHX), polyhexamethylene biguanide (PHBM), and octenidine dihydrochloride (OCT) on cariogenic microorganisms by using their minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). CHX, PHBM, and OCT were diluted in distilled water to the final test concentrations. Using the in-tube dilution method, Streptococcus mutans, Lactobacillus acidophilus, Lactobacillus rhamnosus, and Actinomyces viscosus were cultivated on blood agar and Mueller-Hinton broth (MHB) at 37°C for 48 h. They were read using a spectrophotometer to detect MIC. To determine MBC, samples in the range of the turbidity threshold after 24 h were transferred onto blood agar and evaluated for growth after 24 h. Different MICs and MBCs were observed in all disinfectants against each microorganism. The lowest MIC and MBC against S. mutans (60 mg/L) were obtained from PHBM. The lowest values against L. rhamnosus (15 mg/L, 30 mg/L), A. viscosus (30 mg/L), and L. acidophilus (15 mg/L, 30 mg/L) were determined by OCT. PHBM and OCT have the potential to be replaced with CHX because they were effective against cariogenic microorganisms.

Pyridines/pharmacology , Biguanides/pharmacology , Chlorhexidine/analogs & derivatives , Gram-Positive Bacteria/drug effects , Anti-Infective Agents, Local/pharmacology , Anti-Bacterial Agents/pharmacology , Spectrophotometry , Time Factors , Microbial Sensitivity Tests , Chlorhexidine/pharmacology , Reproducibility of Results , Agar , Dental Caries/microbiology , Gram-Positive Bacteria/growth & development
Braz. oral res. (Online) ; 30(1): e61, 2016. tab, graf
Article in English | LILACS | ID: biblio-952067


Abstract The aim of this study was to analyze the antimicrobial activity and substantivity of Uncaria tomentosa Willd DC (cat's claw, CC) in root dentin contaminated with Enterococcus faecalis. Forty-eight human premolars were contaminated with E. faecalis (ATCC 29212) and randomly divided into four groups according to the irrigant used during chemomechanical preparation (CMP): CC group: 2% CC gel; CHX group: 2% chlorhexidine digluconate gel (CHX); NaOCl group: 5.25% sodium hypochlorite (NaOCl); and SS group: sterile saline (SS). Microbiological samples were collected before (S1) and after (S2) CMP and after 7 days (S3). Colony-forming units (CFU/mL) at the different sampling times and comparisons among the groups were statistically analyzed by Wilcoxon and Kruskal-Wallis tests (p < 0.05). Significant bacterial reduction was achieved in all groups after CMP (p < 0.05). Results show no significant difference between S3 and S2 (p > 0.05) in the CC and CHX groups. Bacterial load was higher in S3 than in S2 samples (p < 0.05) in the NaOCl and SS groups. Our results suggest antibacterial effect of 2% CC gel against E. faecalis in infected dentin, in addition to antibacterial substantivity of 2% CC and 2% CHX up to 7 days.

Humans , Enterococcus faecalis/drug effects , Cat's Claw/chemistry , Dental Pulp Cavity/drug effects , Dental Pulp Cavity/microbiology , Dentin/drug effects , Dentin/microbiology , Anti-Infective Agents/pharmacology , Reference Values , Root Canal Irrigants/pharmacology , Sodium Hypochlorite/pharmacology , Time Factors , Colony Count, Microbial , Random Allocation , Chlorhexidine/analogs & derivatives , Chlorhexidine/pharmacology , Statistics, Nonparametric , Bacterial Load/drug effects
Braz. dent. j ; 26(2): 128-134, Mar-Apr/2015. tab, graf
Article in English | LILACS | ID: lil-741222


The aim of this study was to evaluate the effect of cariogenic challenge on the microtensile bond strength values (μTBS) of dentin pre-treated with chlorhexidine digluconate (CHX) or sodium hypochlorite (NaOCl). Thirty-six sound molars were selected and randomly assigned to 3 dentin pre-treatments (distilled water - control, 2% CHX and 10% NaOCl) and 4 aging protocols (24h control, biofilm without cariogenic challenge, biofilm with cariogenic challenge, and 18-month water storage). The same etch-and-rinse adhesive system and composite resin were used for all groups (n=30 beams). For the biofilm groups, dental microcosm biofilms originated from saliva of a healthy donor were grown on the samples with a defined medium enriched with mucin, with or without 10% sucrose, according to the group. After the experimental period, the microtensile test was performed. Data were analyzed with ANOVA followed by Tukey test (p<0.05). The pre-treatment did not influence μTBS for all aging conditions (p=0.188), but the type of aging affected the bond strength (p<0.001). Cariogenic challenge and water storage aging affected the bond stability resulting in a decrease of the μTBS, but the pre-treatments did not influence the μTBS.

O objetivo neste estudo foi avaliar o efeito do desafio cariogênico na resistência de união (RU) da dentina pré-tratada com clorexidina (CRX) ou hipoclorito de sódio (NaOCl). Trinta e seis molares hígidos foram selecionados e randomizados de acordo com 3 pré-tratamentos dentinários (controle, CRX a 2% e NaOCl a 10%) e 4 protocolos de envelhecimento (controle 24h, biofilme sem desafio cariogênico, biofilme com desafio cariogênico, e armazenamento de 18 meses em água). O mesmo sistema adesivo e resina composta foram usados para todos os grupos (n=30). Biofilme dental de microcosmo originado da saliva de doador saudável foi desenvolvido sobre as amostras com meio enriquecido com mucina adicionada ou não com sacarose a 10%, de acordo com o grupo. Após o período experimental, o teste de microtração foi realizado. Os dados de microtração foram analisados com ANOVA a dois fatores e teste de Tukey (p<0,05). O pré-tratamento não influenciou os valores de RU para todos os protocolos de envelhecimento (p=0,188), porém o tipo de envelhecimento influenciou os valores de RU (p<0,001). O desafio cariogênico e o armazenamento em água afetaram a estabilidade adesiva resultando na diminuição dos valores de RU e o pré-tratamento não influenciou os valores de RU.

Humans , Composite Resins/chemistry , Dental Cements/chemistry , Phosphoric Acids/chemistry , Acid Etching, Dental , Chlorhexidine/analogs & derivatives , Chlorhexidine/pharmacology , Dental Bonding , Dental Restoration, Permanent/methods , Dental Stress Analysis , Dentin/drug effects , In Vitro Techniques , Molar , Sodium Hypochlorite/pharmacology , Surface Properties , Tensile Strength
Journal of Islamic Dental Association of Iran [The]-JIDA. 2015; 26 (4): 256-259
in Persian | IMEMR | ID: emr-167547


An intracanal irrigant is essential for a successful root canal therapy. The aim of this study was to compare the antifungal efficacy of 5.25% sodium hypchlorite, 2% chlorhexidine gluconate and 94% carvacrol against Candida albicans in vitro. In this experimental study, after crown removal and canal preparation of 48 extracted human maxillary centreal incisors, they were randomly divided into 3 experimental groups of 14 teeth, plus 3 teeth as positive and 3 as negative controls. A suspension of Candida albicans [ATCC-10261] was injected into the experimental and positive control group root canals. The teeth were then incubated for 72 hours. Then, each group was rinsed with one of the irrigants for 1 minute and samples were taken from the canals and inoculated on plates. After 48 hours of incubation, the colony growth was assessed and colony forming units [CFUs] served as a measure of antifungal activity. Data were analyzed using the Kruskal Wallis test. In carvacrol, sodium hypochlorite and chlorhexidine gluconate groups, 6, 10 and 1 specimen did not show bacterial growth and the mean CFU was 86.3, 53.3 and 271.2 in the mentioned groups, respectively. Carvacrol and 5.25% sodium hypochlorite had similar antifungal efficacy against Candida albicans and this effect was significantly greater than that 2% chlorhexidine gluconate

Humans , Antifungal Agents , Root Canal Irrigants , Sodium Hypochlorite , Chlorhexidine/analogs & derivatives , Monoterpenes
Braz. oral res. (Online) ; 29(1): 1-6, 2015. tab
Article in English | LILACS | ID: lil-777180


Guedes-Pinto paste is the filling material most employed in Brazil for endodontic treatment of deciduous teeth; however, the Rifocort® ointment has been removed. Thus, the aim of this study was to investigate the antimicrobial potential of filling pastes, by proposing three new pharmacological associations to replace Rifocort® ointment with drugs of already established antimicrobial power: Nebacetin® ointment, 2% Chlorhexidine Gluconate gel, and Maxitrol® ointment. A paste composed of Iodoform, Rifocort® ointment and Camphorated Paramonochlorophenol (CPC) was employed as the gold standard (G1). The other associations were: Iodoform, Nebacetin® ointment and CPC (G2); Iodoform, 2% Chlorhexidine Digluconate gel and CPC (G3); Iodoform, Maxitrol® ointment and CPC (G4). The associations were tested for Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans), Streptococcus oralis (S. oralis), Enterococcus faecalis (E. faecalis), Escherichia coli (E. coli), and Bacillus subtilis (B. subtilis), using the methods of dilution on solid medium – orifice agar – and broth dilution. The results were tested using statistical analysis ANOVA and Kruskal-Wallis. They showed that all the pastes had a bacteriostatic effect on all the microorganisms, without any statistically significant difference, compared with G1. S. aureus was statistically significant (multiple comparison test of Tukey), insofar as G2 and G3 presented the worst and the best performance, respectively. All associations were bactericidal for E. coli, S. aureus, S. mutans and S. oralis. Only G3 and G4 were bactericidal for E. faecalis, whereas no product was bactericidal for B. subtilis. Thus, the tested pastes have antimicrobial potential and have proved acceptable for endodontic treatment of primary teeth.

Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Root Canal Filling Materials/pharmacology , Tooth, Deciduous/drug effects , Analysis of Variance , Bacitracin/pharmacology , Bacteria/growth & development , Chlorhexidine/analogs & derivatives , Chlorhexidine/pharmacology , Drug Combinations , Fluprednisolone/pharmacology , Microbial Sensitivity Tests , Neomycin/pharmacology , Ointments , Polymyxin B/pharmacology , Prednisolone/analogs & derivatives , Prednisolone/pharmacology , Reproducibility of Results , Rifamycins/pharmacology , Statistics, Nonparametric , Time Factors
Braz. oral res. (Online) ; 29(1): 1-7, 2015. tab
Article in English | LILACS | ID: lil-777199


Concern has been raised about the bonding of restorative procedures to an erosive lesion, given the change in organic and inorganic composition and structure of this substrate. This in vitro study evaluated the effect of erosive drinks and an enzyme inhibitor (2% chlorhexidine digluconate – 2% CHX) on bond strength to dentin. Sixty sound human third molars were selected, and the occlusal enamel was flattened, exposing the dentin surface. The specimens were randomly divided into three groups: AS-Artificial saliva (control group), RC- Regular Cola and ZC- Zero Cola. Twenty specimens were immersed in their respective solution for 1 minute, 3 times a day, over the course of 5 days. After acid etching and before bonding with Adper Single Bond 2, half of the samples of each group (n = 10) were treated with 2% CHX, whereas the other half (n = 10) were not, forming the control group (CONV). All the specimens were restored with Filtek Z250 composite resin filled in Tygon tubes (0.48 mm2), yielding six microcylinders for microshear bond strength testing. Three composite resin microcylinders of each specimen were tested after 1 month, and the remaining microcylinders were tested after 6 months. Failure modes were determined using a stereomicroscope (40x). The data were statistically analyzed by three-way ANOVA and Tukey tests (α = 0.05). Overall bonding was reduced after 6 months, regardless of treatment. The 2% CHX enhanced bond strength after 1 month only in the ZC group, and did not enhance bonding performance to demineralized dentin by erosive protocol after 6 months in any group.

Humans , Carbonated Beverages , Chlorhexidine/analogs & derivatives , Chlorhexidine/chemistry , Dentin/drug effects , Enzyme Inhibitors/chemistry , Tooth Erosion/chemically induced , Analysis of Variance , Anti-Infective Agents, Local/chemistry , Composite Resins/chemistry , Dental Restoration Failure , Dental Bonding/methods , Dental Cements/chemistry , Dental Enamel/drug effects , Dentin-Bonding Agents/chemistry , Immersion , In Vitro Techniques , Random Allocation , Saliva, Artificial/chemistry , Shear Strength/drug effects , Surface Properties/drug effects , Time Factors
J. appl. oral sci ; 22(3): 152-158, May-Jun/2014. tab
Article in English | LILACS, BBO | ID: lil-711713


Objectives: The intraoral transmission of cariogenic and periodontopathogenic species seems to be facilitated by contaminated toothbrushes and other oral hygiene devices. The aim of this investigation was to analyze the in vitro retention and survival rate of Streptococcus mutans and Streptococcus sanguinis on different toothbrushes. The impacts of human saliva and antimicrobial toothpaste on these parameters were further evaluated. Material and Methods: Part I: Four toothbrushes (Colgate 360°, Curaprox CS5460 ultra soft, elmex InterX, Trisa Flexible Head3) were contaminated by S. mutans DSM 20523 or S. sanguinis DSM 20068 suspensions for three minutes. Bacteria were removed from the toothbrushes after either three minutes (T0) or 24 hours (T24) of dry storage and grown on Columbia blood agar plates for the quantification of colony-forming units (CFUs). Part II: The effects of saliva from a caries-active or a caries-inactive person and of toothpaste containing 0.12% chlorhexidine digluconate were also tested. Results: Part I: After three minutes of dry storage, approximately one percent of the bacteria were still detectable on the toothbrushes. After 24 hours, S. sanguinis exhibited a more pronounced decrease in viable cell numbers compared with S. mutans but the differences were not significant (Kruskal-Wallis test, p>0.05). Part II: The addition of human saliva from a caries-active or caries-inactive person slightly increased the retention of both streptococcal species at T0. The use of toothpaste had no influence on the amount of viable streptococci at T0, but it reduced the microbial load after 24 hours of storage. There were only slight nonsignificant differences (p>0.05) between the four toothbrushes. Conclusions: In vitro bacterial retention and survival of S. sanguinis and S. mutans on different toothbrushes occurred. Within the limitations of this study, the use of human saliva or an antimicrobial toothpaste ...

Humans , Male , Female , Adult , Dental Devices, Home Care/microbiology , Saliva/microbiology , Streptococcus mutans/growth & development , Streptococcus sanguis/growth & development , Toothbrushing/instrumentation , Toothpastes/pharmacology , Anti-Infective Agents/pharmacology , Bacterial Adhesion , Bacterial Load , Chlorhexidine/analogs & derivatives , Chlorhexidine/pharmacology , Dental Caries/microbiology , Materials Testing , Statistics, Nonparametric , Streptococcus mutans/drug effects , Streptococcus sanguis/drug effects , Surface Properties , Time Factors
Braz. dent. j ; 25(1): 33-37, Jan-Feb/2014. tab, graf
Article in English | LILACS | ID: lil-709399


The objective of this work was to determine the effect of different concentrations of chlorhexidine digluconate (CHX) on setting time, surface hardness, maximum tensile bond strength and antibacterial activity of a glass ionomer cement (GIC). The material used as control was Ketac Molar Easymix GIC. CHX was incorporated into the GIC during its manipulation at concentrations of 0.5, 1.0 and 2.0%. Antimicrobial activity against S. mutans and L. acidophilus was evaluated by means of agar diffusion test. Tensile bond strength data were analyzed statistically using Analysis of variance and Tukey's test. Setting time, Vickers hardness and agar diffusion test were analyzed using Kruskal-Wallis and Mann-Whitney tests at a significance level of 5%. It was observed that adding CHX at concentrations of 1% and 2% increased significantly the setting time of the material (p=0.012 and p=0.003, respectively). There was no significant difference between control and 0.5% CHX groups regarding the setting time. Addition of 2% CHX decreased significantly the surface hardness in relation to the control group (p=0.009), followed by the 1% CHX group (p=0.009). The tensile bond strength of the material also decreased significantly after adding CHX at a concentration of 2% (p=0.001). Addition of CHX promoted formation of an inhibition halo in both bacterial strains for all concentrations. The results showed that the best option for clinical use of GIC with CHX is at 0.5% concentration, since antibacterial activity increased and the physical-mechanical properties remained unchanged.

O objetivo deste trabalho foi determinar o efeito de diferentes concentrações de gluconato de clorexidina (CLX) sobre o tempo de presa, dureza superficial, resistência máxima a tração e atividade antibacteriana de um cimento de ionômero de vidro (CIV). O material utilizado como controle foi o CIV Ketac Molar Easymix. O gluconato de CHX foi incorporado no CIV durante a sua manipulação, em concentrações de 0,5, 1,0 e 2,0%. Atividade antimicrobiana contra S. mutans e L. acidophilus foi avaliada por meio de teste de difusão em ágar. Análise de variância (ANOVA) e teste de Tukey foram usadas para avaliar a resistência a tração do material. Para avaliar tempo de presa, dureza Vickers e teste de difusão em ágar foram usados os testes de Mann-Whitney e Kruskal-Wallis ao nível de significância de 5%. Observou-se que a adição de CHX, em concentrações de 1% e 2%, aumentou significativamente o tempo de presa do material (p = 0,012 e p = 0,003, respectivamente). Não houve diferença significativa entre os grupos controle e CHX 0,5% em relação ao tempo de presa. A adição de 2% de CHX diminuiu significativamente a dureza superficial, em relação ao grupo controle (p = 0,009), seguido pelo grupo 1% de CHX (p = 0,009). A resistência à tração do material também diminuiu significativamente após a adição de CHX a uma concentração de 2% (p = 0,001). A adição de CHX promoveu formação de halo de inibição em ambas as linhagens bacterianas para todas as concentrações. Os resultados mostraram que a melhor opção para o uso clínico de CIV com CHX está numa concentração de 0,5%, uma vez que a atividade antibacteriana aumentada e as propriedades físico-mecânicas permaneceram inalteradas.

Anti-Bacterial Agents/chemistry , Chlorhexidine/analogs & derivatives , Glass Ionomer Cements , Anti-Bacterial Agents/pharmacology , Chlorhexidine/chemistry , Lactobacillus acidophilus/drug effects , Materials Testing , Microbial Sensitivity Tests , Streptococcus mutans/drug effects
Rev. chil. infectol ; 30(4): 361-367, ago. 2013. graf, tab
Article in Spanish | LILACS | ID: lil-690524


Background:Accumulation of bacteria in the pharynx is one of the risk factors of pneumonia due to ventilation. One of the methods of prevention from accumulation of bacteria in the pharynx is the use of oral solutions. Chlorhexidine is considered as the gold standard, but it has various side effects. Aim:Present study was aimed to determine and compare anti-bacterial effects of the chlorhexidine gluconate 0.2%, herbal mouthwash of Matrica® (chamomile extracts) 10%, PersicaTM 10% and normal saline in intensive care unit patients. Methods:In this double blind randomized clinical trial, 80 patients who were admitted in ICU divided into four groups of 20 patients each one. Researchers applied PersicaTM to group one, chlorhexidine gluconate mouth wash 0.2% to group two and third group received Matrica, finally in the control group, normal saline were used. In order to culturing of Staphylococcus aureusand S. pneumoniae,salivary samples were obtained without any stimulation after six minimums oral rinsing. The data were processed in SPSS17 software and analyzed by appropriate statistical tests. Results:Decreased rate of bacterial colonies after intervention in the whole four groups was significant (p < 0.001). The mouth wash of chlorhexidine (p < 0.001), PersicaTM (p: 0.008) and Matrica (p: 0.01) had a significant antibacterial effect on S. aureusand S. pneumoniae(p < 0.001). Conclusion:Herbal oral mouthwash of persica and matrica has the effect on S. pneumoniae and S. aureusof oropharynx area in mechanically ventilated patients. However, there is a need for further research to be considered as an alternative to chlorhexidine for prevention of VALP in ICU patients.

Introducción:La presencia y acumulación de bacterias en la cavidad oral es un factor de riesgo para el desarrollo de neumonía asociada a ventilación mecánica. Uno de los métodos para prevenir esta acumulación en la faringe es el uso de soluciones orales. Al respecto, clorhexidina es considerada el estándar de oro, pero tiene varios efectos colaterales. Objetivo:Determinar y comparar el efecto antibacteriano de gluconato de chorhexidina al 0,2%, aseo bucal con preparado en base a hierba matrica (extracto de chamomile) al 10%, Persica® al 10% y solución salina fisiológica (NaCl 9%0) en pacientes de la unidad de cuidados intensivos ingresados para ventilación mecánica. Métodos:Ensayo doble ciego, randomizado, sobre 80 pacientes ingresados en UCI, divididos en cuatro grupos de 20 miembros cada uno. El grupo I recibió Persica®, el grupo II aseo bucal con gluconato de clorhexidina al 0,2%, el grupo III recibió matrica y, finalmente, el grupo IV-control recibió solución salina fisiológica. Con la finalidad de cultivar Staphylococcus aureus yS. pneumoniae,se obtuvieron muestras de saliva sin efectuar estímulo alguno tras un mínimo de seis lavados bucales. Los datos fueron procesados en el software SPSS17 y analizados por tests estadísticos apropiados. Resultados:La disminución en el recuento de colonias bacterianas, después de la intervención, fue significativa en los cuatro grupos (p < 0,001). El aseo bucal con clorhexidina (p < 0,001), Persica® (p: 0,008) y matrica (p: 0,01) tuvo un significativo efecto antibacteriano sobre las especies S. aureusy S. pneumoniae(p < 0,001). Conclusión:El aseo bucal con solución en base a hierbas de Persica® y matrica tiene un efecto sobre S. pneumoniae y S. aureusde la cavidad oral en pacientes en régimen de ventilación mecánica. No obstante, se requiere de mayores estudios para considerarlo una alternativa a clorhexidina para la prevención de neumonías en pacientes de UCI.

Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Anti-Bacterial Agents/administration & dosage , Chlorhexidine/analogs & derivatives , Mouthwashes/administration & dosage , Plant Preparations/administration & dosage , Pneumonia, Ventilator-Associated/prevention & control , Prunus/chemistry , Anti-Bacterial Agents/pharmacology , Colony Count, Microbial , Chlorhexidine/administration & dosage , Chlorhexidine/pharmacology , Double-Blind Method , Mouthwashes/pharmacology , Plant Preparations/pharmacology , Pneumonia, Ventilator-Associated/microbiology , Staphylococcus aureus/drug effects , Staphylococcus aureus/growth & development , Staphylococcus aureus/isolation & purification , Streptococcus pneumoniae/drug effects , Streptococcus pneumoniae/growth & development , Streptococcus pneumoniae/isolation & purification
Braz. dent. j ; 24(3): 188-193, May-Jun/2013. tab, graf
Article in English | LILACS | ID: lil-681867


This study investigated the expression of extracellular matrix glycoproteins tenascin (TN) and fibronectin (FN) in pulp repair after capping with calcium hydroxide (CH), following different hemostasis protocols. Class I cavities with a pulp exposure were prepared in 42 human third molars scheduled for extraction. Different hemostatic agents (0.9% saline solution, 5.25% sodium hypochlorite and 2% chlorhexidine digluconate) were used and pulps were capped with CH cement. After 7, 30 or 90 days, teeth were extracted, formalin-fixed, and prepared for immunohistochemical technique. Hemostatic agents did not influence the expression of TN and FN. Both glycoproteins were found in the entire the pulp tissue and around collagen fibers, but were absent in the mineralized tissues. In the predentin, TN showed positive immunostaining and FN had a variable expression. Within 7 days post-treatment, a slightly more pronounced immunostaining on the pulp exposure site was observed. Within 30 days, TN and FN demonstrated a positive expression around the dentin barrier and at 90 days, a thin and linear expression of TN and FN was delimitating the reparative dentin. In conclusion, hemostatic agents did not influence TN and FN expression. Immunostaining for TN and FN was seen in different regions and periods, demonstrating their role in pulp repair.

Este estudo investigou a expressão das glicoproteínas Tenascina (TN) e Fibronectina (FN) da matriz extracelular no reparo pulpar após capeamento com hidróxido de cálcio (HC), seguindo diferentes protocolos de hemostasia. Cavidades de classe I com exposição pulpar foram preparadas em 42 terceiros molares humanos indicados para extração. Diferentes agentes hemostáticos (solução salina a 0,9%, hipoclorito de sódio a 5,25% e clorexidina a 2%) foram usados e as polpas foram capeadas com cimento de HC. Após 7, 30 ou 90 dias, os dentes foram extraídos, fixados em formalina e preparados para análise imunoistoquímica. Os agentes hemostáticos não influenciaram a expressão de TN e FN. Ambas glicoproteínas foram encontradas em todo tecido pulpar, ao redor das fibras colágenas e estiveram ausentes nos tecidos mineralizados. Na pré-dentina, a TN mostrou forte imunoexpressão e a FN teve uma expressão variável. Após 7 dias, foi observada uma expressão levemente mais pronunciada no lugar da exposição pulpar. Aos 30 dias, a TN e a FN demonstraram uma expressão mais forte sob a barreira dentinária e aos 90 dias, uma expressão fina e linear da TN e FN apresentava-se delimitando a dentina reparativa. Em conclusão, os agentes hemostáticos não influenciaram e expressão da TN e da FN. A imunoexpressão da TN e FN foi observada em diferentes regiões e períodos, demonstrando o seu papel no reparo pulpar.

Adult , Humans , Young Adult , Dental Pulp Capping , Fibronectins/analysis , Hemostatics/therapeutic use , Pulp Capping and Pulpectomy Agents/therapeutic use , Tenascin/analysis , Bisphenol A-Glycidyl Methacrylate/chemistry , Calcium Hydroxide/therapeutic use , Chlorhexidine/analogs & derivatives , Chlorhexidine/therapeutic use , Collagen/analysis , Composite Resins/chemistry , Dental Pulp Exposure/therapy , Dental Pulp/chemistry , Dental Restoration, Permanent/methods , Dentin, Secondary/chemistry , Dentin/chemistry , Follow-Up Studies , Sodium Chloride/therapeutic use , Sodium Hypochlorite/therapeutic use , Tooth Extraction
Braz. oral res ; 26(6): 523-529, Nov.-Dec. 2012. ilus, tab
Article in English | LILACS | ID: lil-656704


Volatile sulphur compounds (VSC) are the gases mainly responsible for halitosis (bad breath). The aim of this research was to evaluate the effects of medicinal plants on halitosis control. Two commonly used plants were tested: Curcuma zedoaria and Camellia sinensis (green tea). These plants were prepared as an aqueous solution and used as mouthwashes, compared with a standard mouthwash of 0.12% chlorhexidine gluconate and a placebo (water). The experiment was conducted with 30 volunteers from the School of Dentistry of São Jose dos Campos, Univ. Estadual Paulista - UNESP, SP, Brazil. Each volunteer tested the four mouthwashes. The Cysteine Challenge Method, modified for this study, was used for initial breath standardization. Four breath assessments were conducted after volunteers rinsed orally with acetylcysteine: one before the test mouthwash was used; the second, one minute after its use; a third 90 minutes later; and the last 180 minutes later. The results showed that chlorhexidine gluconate lowered VSC production immediately, and that this effect lasted up to 3 hours, while the tested plants had immediate inhibitory effects but no residual inhibitory effects on VSC. We concluded that Curcuma zedoaria and Camellia sinensis, prepared as infusions and used as mouthwashes, did not have a residual neutralizing effect on VSC.

Adult , Female , Humans , Male , Camellia sinensis/chemistry , Curcuma/chemistry , Halitosis/prevention & control , Mouthwashes/therapeutic use , Analysis of Variance , Brazil , Chlorhexidine/analogs & derivatives , Chlorhexidine/therapeutic use , Sulfur Compounds/chemistry , Time Factors , Treatment Outcome
Braz. dent. j ; 23(5): 521-526, Sept.-Oct. 2012. ilus, tab
Article in English | LILACS | ID: lil-660354


The aim of this study was to evaluate the effects of different irrigants on sealer-dentin bond strength when using Real Seal. Thirty single-rooted teeth were divided into 3 groups. In one group, the teeth were irrigated with 3 mL of 2.5% NaOCl after each file change, flushed with 17% EDTA for 3 min and finally rinsed with 3 mL of 2.5% NaOCl. In the other two groups, rinse with NaOCl was replaced with 2% chlorhexidine gluconate (CHX) and 0.9% saline, respectively. Each root was sectioned transversally into apical, middle and coronal thirds to obtain 2-mm-thick slices. Each slice was filled with Real Seal and Resilon. Push-out test was used to analyze bond strength and failure modes were classified as adhesive, cohesive or mixed, according to SEM observations. The push-out test did not reveal any statistically significant difference (p>0.05) between the irrigants. However, the groups exhibited significantly different (p<0.05) bond strengths in terms of the root canal third. Higher bond strength was observed at the apical third when compared with coronal third, while middle third presented intermediary values. Fifteen specimens were analyzed by SEM (5 per group). Eleven specimens exhibited adhesive failures (5 in saline, 4 in NaOCl and 2 in CHX group); 2 cohesive failures were observed in the CHX group, and 1 mixed failure each was observed in the CHX and NaOCl groups. The tested irrigants did not influence the bond strength of Resilon and Real Seal to dentin. The apical third exhibited higher mean bond strengths and adhesive failures were predominant.

O objetivo deste estudo foi avaliar os efeitos de diferentes soluções irrigadoras na resistência adesiva do Real Seal à dentina radicular. Trinta dentes monorradiculares foram divididos em 3 grupos: Grupo 1 - os dentes foram irrigados com 3 mL de NaOCl 2,5% após cada troca de instrumento, irrigados com EDTA 17% por 3 min e lavagem final com NaOCl 2,5%. Nos Grupos 2 e 3, o NaOCl foi substituído por digluconato de clorexidina 2% (CHX) e solução salina 0,9%, respectivamente. Cada raiz foi seccionada transversalmente nos terços apical, médio e cervical para obter fatias de 2 mm de espessura. Cada slice foi obturado com Real Seal e Resilon. Teste de push-out foi usado para analisar a resistência adesiva e os padrões de falha foram classificados como adesiva, coesiva e mista de acordo com MEV. O teste de push-out não revelou diferença estatisticamente significante entre os irrigantes (p<0,05). Porém, os grupos exibiram diferentes valores de resistência adesiva de acordo com o terço radicular (p>0,05). Maior resistência adesiva foi observada na região apical quando comparado com o terço cervical, enquanto que o terço médio apresentou valores intermediarios. Quinze espécimes foram analisados através de MEV (5 por grupo). Onze espécimes exibiram falhas adesivas (5 no grupo da solução salina, 4 no grupo NaOCl e 2 na CHX); duas falhas coesivas foram observadas no grupo CHX e uma falha mista foi observada no grupo da CHX e uma no NaOCl. Os irrigantes testados não influenciaram a resistência adesiva do Resilon e do Real Seal à dentina radicular. O terço apical apresentou maior resistência adesiva e falhas adesivas foram predominantes para todos os grupos.

Humans , Chlorhexidine/analogs & derivatives , Dental Stress Analysis/methods , Dentin/chemistry , Root Canal Filling Materials/chemistry , Sodium Hypochlorite/chemistry , Analysis of Variance , Dental Bonding , Equipment Failure Analysis , Microscopy, Electron, Scanning , Root Canal Irrigants/chemistry
Braz. dent. j ; 23(4): 387-393, 2012. tab
Article in English | LILACS | ID: lil-658015


Chemical disinfectants are usually associated with mechanical methods to remove stains and reduce biofilm formation. This study evaluated the effect of disinfectants on release of metal ions and surface roughness of commercially pure titanium, metal alloys, and heat-polymerized acrylic resin, simulating 180 immersion trials. Disk-shaped specimens were fabricated with commercially pure titanium (Tritan), nickel-chromium-molybdenum-titanium (Vi-Star), nickel-chromium (Fit Cast-SB Plus), and nickel-chromium-beryllium (Fit Cast-V) alloys. Each cast disk was invested in the flasks, incorporating the metal disk to the heat-polymerized acrylic resin. The specimens (n=5) were immersed in these solutions: sodium hypochlorite 0.05%, Periogard, Cepacol, Corega Tabs, Medical Interporous, and Polident. Deionized water was used as a control. The quantitative analysis of metal ion release was performed using inductively coupled plasma mass spectrometry (ELAN DRC II). A surface analyzer (Surftest SJ-201P) was used to measure the surface roughness (µm). Data were recorded before and after the immersions and evaluated by two-way ANOVA and Tukey's test (α=0.05). The nickel release proved most significant with the Vi-Star and Fit Cast-V alloys after immersion in Medical Interporous. There was a significant difference in surface roughness of the resin (p=0.011) after immersion. Cepacol caused significantly higher resin roughness. The immersion products had no influence on metal roughness (p=0.388). It could be concluded that the tested alloys can be considered safe for removable denture fabrication, but disinfectant solutions as Cepacol and Medical Interporous tablet for daily denture immersion should be used with caution because it caused greater resin surface roughness and greater ion release, respectively.

Desinfetantes químicos são normalmente associados a métodos mecânicos para remover manchas e reduzir a formação do biofilme. Este estudo avaliou o efeito de desinfetantes na liberação de íons metálicos e na rugosidade superficial do titânio comercialmente puro, ligas metálicas e resina acrílica termopolimerizável, simulando 180 ensaios de imersões. Espécimes em formato de discos foram confeccionados com titânio comercialmente puro (Tritan), liga de níquel-cromo-molibdênio-titânio (Vi-Star), liga de níquel-cromo (Fit Cast-SB Plus) e liga de níquel-cromo-berílio (Fit Cast-V). Os espécimes (n=5) foram imersos nestas soluções: hipoclorito de sódio a 0,05%, Periogard, Cepacol, Corega Tabs, Medical Interporous e Polident. Como controle, foi utilizada a água deionizada. A análise quantitativa de liberação de íons metálicos foi realizada por meio de espectrometria de massa com plasma indutivamente acoplado (ELAN DRC II). O rugosímetro (Surftest SJ-201P) foi utilizado para medir a rugosidade superficial (µm). Os dados foram registrados antes e depois das imersões e avaliados por ANOVA com dois fatores e teste de Tukey (α=0,05). A liberação de níquel provou ser mais expressiva nas ligas Vi-Star e Fit Cast-V após a imersão em Medical Interporous. Houve diferença significante na rugosidade superficial da resina (p=0,011) após a imersão. O Cepacol causou maior rugosidade superficial de forma significativa. Os produtos de imersão não influenciaram nos resultados da rugosidade do metal (p=0,388). Pode-se concluir que as ligas metálicas testadas podem ser consideradas seguras para a fabricação de próteses removíveis, mas as soluções desinfetantes como o Cepacol e a pastilha Medical Interporous para a imersão diária da prótese devem ser utilizados com cautela, pois causaram maior rugosidade superficial da resina e maior liberação de íons, respectivamente.

Humans , Denture Bases , Dental Alloys/chemistry , Denture Cleansers/chemistry , Acrylic Resins/chemistry , Alloys/chemistry , Aluminum/analysis , Beryllium/analysis , Borates/chemistry , Cetylpyridinium/chemistry , Chlorhexidine/analogs & derivatives , Chlorhexidine/chemistry , Chromium Alloys/chemistry , Chromium/analysis , Citric Acid/chemistry , Dental Disinfectants/chemistry , Dental Materials/chemistry , Materials Testing , Metals/analysis , Metals/chemistry , Molybdenum/analysis , Nickel/analysis , Spectrophotometry, Atomic , Surface Properties , Sodium Hypochlorite/chemistry , Sulfates/chemistry , Titanium/analysis , Titanium/chemistry
Braz. dent. j ; 23(4): 417-421, 2012. ilus, tab
Article in English | LILACS | ID: lil-658020


This study compared the surface detail reproduction and dimensional accuracy of stone models obtained from molds disinfected with 2% sodium hypochlorite, 2% chlorhexidine digluconate or 0.2% peracetic acid to models produced using molds which were not disinfected, with 3 alginate materials (Cavex ColorChange, Hydrogum 5 and Jeltrate Plus). The molds were prepared over matrix containing 20-, 50-, and 75-µm lines, performed under pressure with perforated metal tray. The molds were removed following gelation and either disinfected (using one of the solutions by spraying followed by storage in closed jars for 15 min) or not disinfected. The samples were divided into 12 groups (n=5). Molds were filled with dental gypsum Durone IV and 1 h after the start of the stone mixing the models were separated from the tray. Surface detail reproduction and dimensional accuracy were evaluated using optical microscopy on the 50-µm line with 25 mm in length, in accordance with the ISO 1563 standard. The dimensional accuracy results (%) were subjected to ANOVA. The 50 µm-line was completely reproduced by all alginate impression materials regardless of the disinfection procedure. There was no statistically significant difference in the mean values of dimensional accuracy in combinations between disinfectant procedure and alginate impression material (p=0.2130) or for independent factors. The disinfectant solutions and alginate materials used in this study are no factors of choice regarding the surface detail reproduction and dimensional accuracy of stone models.

Este estudo comparou a reprodução de detalhes da superfície e alteração dimensional de modelos de gesso obtidos a partir de moldes desinfetados com hipoclorito de sódio 2%, digluconato de clorexidina 2%, ou ácido peracético 0,2% a modelos confeccionados utilizando moldes que não foram desinfetados com três alginatos (Cavex ColorChange, Hydrogum 5, Jeltrate Plus). Os moldes foram preparados sobre matriz contendo linhas de 20, 50 e 75 µm realizado sob pressão com moldeira de metal perfurada. Os moldes foram removidos após a geleificação e desinfetados (utilizando uma das soluções por pulverização, armazenados em frascos fechados durante 15 min) ou não desinfetados. Assim, as amostras foram divididas em 12 grupos (n=5). Os moldes foram preenchidos com gesso dental Durone IV e uma hora após a manipulação do gesso os modelos foram separados da moldeira. A reprodução de detalhes da superfície e a precisão dimensional foram avaliadas usando microscopia óptica na linha 50 µm com 25 mm de comprimento, de acordo com a norma ISO 1563. Os resultados de precisão dimensional (%) foram submetidos à ANOVA. A linha de 50 µm foi completamente reproduzida por todos os alginatos, independentemente do processo de desinfecção. Não houve diferença estatisticamente significativa nos valores médios de precisão dimensional nas combinações entre procedimento de desinfecção e alginato (p=0,2130), ou para fatores independentes. Soluções desinfetantes e alginatos utilizados neste estudo não são fatores de escolha em relação à reprodução de detalhes da superfície e alteração dimensional de modelos de gesso.

Humans , Alginates/chemistry , Calcium Sulfate/chemistry , Models, Dental , Dental Disinfectants/chemistry , Dental Impression Materials/chemistry , Chlorhexidine/analogs & derivatives , Chlorhexidine/chemistry , Colloids/chemistry , Dental Impression Technique/instrumentation , Materials Testing , Microscopy , Organic Chemicals/chemistry , Pressure , Peracetic Acid/chemistry , Surface Properties , Sodium Hypochlorite/chemistry , Time Factors