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1.
Int. braz. j. urol ; 45(6): 1113-1121, Nov.-Dec. 2019. tab, graf
Article in English | LILACS | ID: biblio-1056346

ABSTRACT

ABSTRACT Purpose: To establish whether the citrate concentration in the seminal fluid ([CITRATE]) measured by means of high-resolution nuclear magnetic resonance spectroscopy (1HNMRS) is superior to the serum prostate-specific antigen (PSA) concentration in detecting of clinically significant prostate cancer (csPCa) in men with persistently elevated PSA. Materials and Methods: The group of patients consisted of 31 consecutively seen men with histological diagnosis of clinically localized csPCa. The control group consisted of 28 men under long-term follow-up (mean of 8.7 ± 3.0 years) for benign prostate hyperplasia (BPH), with persistently elevated PSA (above 4 ng/mL) and several prostate biopsies negative for cancer (mean of 2.7 ± 1.3 biopsies per control). Samples of blood and seminal fluid (by masturbation) for measurement of PSA and citrate concentration, respectively, were collected from patients and controls. Citrate concentration in the seminal fluid ([CITRATE]) was determined by means of 1HNMRS. The capacities of PSA and [CITRATE] to predict csPCa were compared by means of univariate analysis and receiver operating characteristic (ROC) curves. Results: Median [CITRATE] was significantly lower among patients with csPCa compared to controls (3.93 mM/l vs. 15.53 mM/l). There was no significant difference in mean PSA between patients and controls (9.42 ng/mL vs. 8.57 ng/mL). The accuracy of [CITRATE] for detecting csPCa was significantly superior compared to PSA (74.8% vs. 54.8%). Conclusion: Measurement of [CITRATE] by means of 1HNMRS is superior to PSA for early detection of csPCa in men with elevated PSA.


Subject(s)
Humans , Male , Aged , Prostatic Neoplasms/diagnosis , Semen/chemistry , Prostate-Specific Antigen/blood , Citric Acid/analysis , Prostatic Hyperplasia/diagnosis , Prostatic Hyperplasia/blood , Prostatic Neoplasms/pathology , Prostatic Neoplasms/blood , Biopsy , Biomarkers, Tumor/analysis , Reproducibility of Results , Sensitivity and Specificity , Statistics, Nonparametric , Risk Assessment , Middle Aged
2.
Biol. Res ; 48: 1-8, 2015. tab
Article in English | LILACS | ID: lil-734615

ABSTRACT

BACKGROUND: The Eurasian grapevine (Vitis vinifera L.) is the most widely cultivated and economically important horticultural crop in the world. As a one of the origin area, Anatolia played an important role in the diversification and spread of the cultivated form V. vinifera ssp. vinifera cultivars and also the wild form V. vinifera ssp. sylvestris ecotypes. Although several biodiversity studies have been conducted with local cultivars in different regions of Anatolia, no information has been reported so far on the biochemical (organic acids, sugars, phenolic acids, vitamin C) and antioxidant diversity of local historical table V. vinifera cultivars grown in Igdir province. In this work, we studied these traits in nine local table grape cultivars viz. 'Beyaz Kismis' (synonym name of Sultanina or Thompson seedless), 'Askeri', 'El Hakki', 'Kirmizi Kismis', 'Inek Emcegi', 'Hacabas', 'Kerim Gandi', 'Yazen Dayi', and 'Miskali' spread in the Igdir province of Eastern part of Turkey. RESULTS: Variability of all studied parameters is strongly influenced by cultivars (P < 0.01). Among the cultivars investigated, 'Miskali' showed the highest citric acid content (0.959 g/l) while 'Kirmizi Kismis' produced predominant contents in tartaric acid (12.71 g/l). The highest glucose (16.47 g/100 g) and fructose (15.55 g/100 g) contents were provided with 'Beyaz Kismis'. 'Kirmizi Kismis' cultivar had also the highest quercetin (0.55 mg/l), o-coumaric acid (1.90 mg/l), and caffeic acid (2.73 mg/l) content. The highest ferulic acid (0.94 mg/l), and syringic acid (2.00 mg/l) contents were observed with 'Beyaz Kismis' cultivar. The highest antioxidant capacity was obtained as 9.09 μmol TE g-1 from 'Inek Emcegi' in TEAC (Trolox equivalent Antioxidant Capacity) assay. 'Hacabas' cultivar had the highest vitamin C content of 35.74 mg/100 g. CONCLUSIONS: Present results illustrated that the historical table grape cultivars grown in Igdir province of Eastern part of Turkey contained diverse and valuable sugars, organic acids, phenolic acids, Vitamin C values and demonstrated important antioxidant capacity for human health benefits. Further preservation and use of this gene pool will be helpful to avoid genetic erosion and to promote continued agriculture in the region.


Subject(s)
Citric Acid/analysis , Dietary Carbohydrates/analysis , Fruit/chemistry , Polyphenols/analysis , Vitis/chemistry , Acids/analysis , Antioxidants/analysis , Ascorbic Acid/analysis , Caffeic Acids/analysis , Coumaric Acids/analysis , Crops, Agricultural/chemistry , Crops, Agricultural/classification , Gallic Acid/analysis , Gallic Acid/analogs & derivatives , Hydroxybenzoates/analysis , Turkey , Tartrates/analysis , Vitis/classification
3.
Biol. Res ; 47: 1-5, 2014. tab
Article in English | LILACS | ID: biblio-950717

ABSTRACT

BACKGROUND: The Hawthorn (Crateagus sp.) mostly occurs around the temperate region of the world with a high number of species, producing a fruit with numerous beneficial effects for human health. The aim of the study was to determine organic acid and sugar contents in the fruit of a number of hawthorn species grown in Erzincan province of Turkey. RESULTS: Citric acid was the predominant organic acid in all hawthorn species and C. pseudoheterophylla had the highest citric acid content (23.688 g/100 g). There were not statistically significant differences among hawthorn species (except C. atrosanguinea Pojark) in terms of fumaric acid content. C. pontica C.Koch had a higher content of vitamin C (9.418 mg/100 g) compared to other species. Fructose was the predominant sugar component in all species and C. monogyna subsp. monogyna Joiq had the highest fructose content (18.378 g/100 g). CONCLUSIONS: The high fruit quality of the studied species indicates the importance of this fruit in human nutrition as a natural source. The study revealed that there were differences in terms of fruit characteristics among hawthorn species and thus better quality hawthorn genotypes can be selected within the species. Hence, this study is considered to be a valuable reference for forthcoming studies. The high fruit quality of the studied species indicates the importance of this fruit in human nutrition as a natural source.


Subject(s)
Ascorbic Acid/analysis , Citric Acid/analysis , Crataegus/chemistry , Fructose/analysis , Fruit/chemistry , Nutritive Value , Sucrose/analysis , Turkey , Dietary Carbohydrates/analysis , Plant Extracts/analysis , Crops, Agricultural/chemistry , Dicarboxylic Acids/analysis , Glucose/analysis
4.
J. appl. oral sci ; 20(3): 376-383, May-June 2012. ilus, tab
Article in English | LILACS | ID: lil-643737

ABSTRACT

OBJECTIVES: The aim of this study was to establish the parameters of concentration, time and mode of application of citric acid and sodium citrate in relation to root conditioning. MATERIAL AND METHODS: A total of 495 samples were obtained and equally distributed among 11 groups (5 for testing different concentrations of citric acid, 5 for testing different concentrations of sodium citrate and 1 control group). After laboratorial processing, the samples were analyzed under scanning electron microscopy. A previously calibrated and blind examiner evaluated micrographs of the samples. Non-parametric statistical analysis was performed to analyze the data obtained. RESULTS: Brushing 25% citric acid for 3 min, promoted greater exposure of collagen fibers in comparison with the brushing of 1% citric acid for 1 minute and its topical application at 1% for 3 min. Sodium citrate exposed collagen fibers in a few number of samples. CONCLUSION: Despite the lack of statistical significance, better results for collagen exposure were obtained with brushing application of 25% citric acid for 3 min than with other application parameter. Sodium citrate produced a few number of samples with collagen exposure, so it is not indicated for root conditioning.


Subject(s)
Humans , Acid Etching, Dental/methods , Chelating Agents/administration & dosage , Citrates/administration & dosage , Citric Acid/administration & dosage , Dental Cavity Preparation/methods , Tooth Root/drug effects , Chelating Agents/analysis , Citrates/analysis , Citric Acid/analysis , Collagen/drug effects , Dental Cavity Preparation/instrumentation , Microscopy, Electron, Scanning , Smear Layer , Time Factors
5.
Braz. j. microbiol ; 42(1): 394-409, Jan.-Mar. 2011. ilus, tab
Article in English | LILACS | ID: lil-571414

ABSTRACT

Valencia orange (Citrus sinensis) peel was employed in this work as raw material for the production of citric acid (CA) by solid-state fermentation (SSF) of Aspergillus niger CECT-2090 (ATCC 9142, NRRL 599) in Erlenmeyer flasks. To investigate the effects of the main operating variables, the inoculum concentration was varied in the range 0.5À10³ to 0.7À10(8) spores/g dry orange peel, the bed loading from 1.0 to 4.8 g of dry orange peel (corresponding to 35-80 percent of the total volume), and the moisture content between 50 and 100 percent of the maximum water retention capacity (MWRC) of the material. Moreover, additional experiments were done adding methanol or water in different proportions and ways. The optimal conditions for CA production revealed to be an inoculum of 0.5À10(6) spores/g dry orange peel, a bed loading of 1.0 g of dry orange peel, and a humidification pattern of 70 percent MWRC at the beginning of the incubation with posterior addition of 0.12 mL H2O/g dry orange peel (corresponding to 3.3 percent of the MWRC) every 12 h starting from 62 h. The addition of methanol was detrimental for the CA production. Under these conditions, the SSF ensured an effective specific production of CA (193 mg CA/g dry orange peel), corresponding to yields of product on total initial and consumed sugars (glucose, fructose and sucrose) of 376 and 383 mg CA/g, respectively. These results, which demonstrate the viability of the CA production by SSF from orange peel without addition of other nutrients, could be of interest to possible, future industrial applications.


Subject(s)
Citric Acid/analysis , Aspergillus niger/isolation & purification , Citrus , Citrus sinensis , Fermentation , Methanol/analysis , Pectins , Volatile Solids , Food Samples , Methods , Carbonated Beverages , Methods
6.
Braz. dent. j ; 22(6): 473-478, 2011. tab
Article in English | LILACS | ID: lil-622720

ABSTRACT

The aim of this study was to create a synthetic juice (SJ) to be used as a surrogate for natural orange juices in erosion studies, verifying its erosive potential. The SJ was formulated based on the chemical composition of orange juices from different locations. Forty enamel and 40 root dentin specimens were randomly assigned into 4 experimental groups (n=10): SJ; 1% Citric Acid (CA); Minute Maid Original® (MM) and Florida Natural Original® (FN). The specimens were immersed in their respective solutions for 5 min, 6x/day for 5 days, in an erosion-remineralization cycling model. Enamel specimens were analyzed by surface Knoop microhardness and optical profilometry and dentin specimens only by optical profilometry. Outcomes were analyzed statistically by ANOVA followed by Tukey’s test considering a significance level of 5%. For enamel, the surface loss and microhardness changes found for MM and SJ groups were similar (p>0.05) and significantly lower (p<0.01) than those found in the CA group. For dentin, CA promoted significantly greater (p<0.01) surface loss compared with all the other groups. No significant difference (p>0.05) was observed in dentin surface loss between MM and SJ. In conclusion, CA was the most erosive solution, and SJ had a similar erosive potential to that of MM natural orange juice.


O objetivo deste estudo foi criar um suco sintético (SJ) para ser usado como substituto do suco de laranja natural em estudos de erosão dental, verificando o seu potencial erosivo. O SJ foi formulado com base na composição química de sucos de laranja de diferentes locais. Quarenta espécimes de esmalte e 40 de dentina radicular foram aleatoriamente alocados em 4 grupos experimentais (n=10): SJ; 1% Citric acid (CA); Minute Maid Original® (MM) e Florida Natural Original® (FN). Os espécimes foram imersos nas suas respectivas soluções por 5 min, 6x/dia por 5 dias, em um modelo de ciclagem de erosão-remineralização. Os espécimes de esmalte foram analisados por microdureza de superfície Knoop e perfilometria ótica, enquanto que os espécimes de dentina foram analisados somente por perfilometria. Os resultados foram analisados estatisticamente com o teste de ANOVA, seguido pelo teste de Tukey, considerando um nível de significância de 5%. Para o esmalte, a perda superficial e as alterações de microdureza encontradas para os grupos MM e SJ foram similares (p>0,05) e significantemente menores (p<0,01) do que as encontradas para o grupo CA. Para dentina, CA promoveu significantemente (p<0,01) a maior perda de superfície quando comparada aos outros grupos. Não foram encontradas diferenças significantes (p>0,05) entre a perda de superfície de dentina dos grupos MM e SJ. Concluiu-se que CA foi a solução mais erosiva e SJ apresentou um potencial erosivo semelhante ao do suco de laranja natural MM.


Subject(s)
Humans , Beverages , Citrus sinensis , Fruit , Tooth Erosion/etiology , Ascorbic Acid/analysis , Beverages/analysis , Chemistry, Pharmaceutical , Calcium/analysis , Citric Acid/analysis , Citric Acid/pharmacology , Citrus sinensis/chemistry , Dental Enamel/drug effects , Dental Enamel/pathology , Dentin/drug effects , Dentin/pathology , Fruit/chemistry , Glucose/analysis , Hardness , Materials Testing , Magnesium/analysis , Phosphorus/analysis , Potassium/analysis , Sucrose/analysis , Time Factors , Tooth Remineralization
7.
Int. braz. j. urol ; 35(3): 299-309, May-June 2009. graf, tab
Article in English | LILACS | ID: lil-523155

ABSTRACT

INTRODUCTION: Studies that compare the impact of different infectious entities of the male reproductive tract (MRT) on the male accessory gland function are controversial. MATERIAL AND METHODS: Semen analyses of 71 patients with proven infections of the MRT were compared with the results of 40 healthy non-infected volunteers. Patients were divided into 3 groups according to their diagnosis: chronic prostatitis NIH type II (n = 38), chronic epididymitis (n = 12), and chronic urethritis (n = 21). RESULTS: The bacteriological analysis revealed 9 different types of microorganisms, considered to be the etiological agents, isolated in different secretions, including: urine, expressed prostatic secretions, semen and urethral smears: E. Coli (n = 20), Klebsiella (n = 2), Proteus spp. (n = 1), Enterococcus (n = 20), Staphylococcus spp. (n = 1), M. tuberculosis (n = 2), N. gonorrhea (n = 8), Chlamydia tr. (n = 16) and, Ureaplasma urealyticum (n = 1). The infection group had significantly (p < 0.05) lower: semen volume, alpha-glucosidase, fructose, and zinc in seminal plasma and, higher pH than the control group. None of these parameters was sufficiently accurate in the ROC analysis to discriminate between infected and non-infected men. CONCLUSION: Proven bacterial infections of the MRT impact negatively on all the accessory gland function parameters evaluated in semen, suggesting impairment of the secretory capacity of the epididymis, seminal vesicles and prostate. These findings were associated with an infectious related significant increase of semen pH. None of the semen parameters evaluated can be suggested as a diagnostic tool for infection.


Subject(s)
Adult , Humans , Male , Middle Aged , Young Adult , Bacterial Infections/complications , Genital Diseases, Male/diagnosis , Genitalia, Male , Semen , Biomarkers/analysis , Case-Control Studies , Chronic Disease , Citric Acid/analysis , Ejaculation/physiology , Epididymitis/diagnosis , Epididymitis/physiopathology , Fructose/analysis , Genital Diseases, Male/microbiology , Genital Diseases, Male/physiopathology , Infertility, Male/microbiology , Prostate/physiopathology , Prostate , Prostatitis/diagnosis , Prostatitis/physiopathology , Semen/chemistry , Semen/microbiology , Seminal Vesicles , Urethritis/diagnosis , Young Adult , alpha-Glucosidases/analysis
8.
J. bras. nefrol ; 28(2): 72-76, jun. 2006. ilus, tab
Article in Portuguese | LILACS | ID: lil-607396

ABSTRACT

Introdução: A hipercalciúria é comumente associada à nefrolitíase. Porém, o risco relacionado à formação de cálculos renais é pouco conhecido. Objetivo: Comparar a demografia e a evolução clínica de pacientes com nefrolitíase e hipercalciúria (HC) com pacientes portadores de outros distúrbios metabólicos(NH). Métodos: Foram estudados 155 pacientes com apenas um distúrbio metabólico e que apresentaram seguimento mínimo de um ano. Foram dosados em duas amostras de urina de 24 horas: cálcio, creatinina, sódio, ácido úrico, citrato, oxalato. A avaliação radiológica foi feita com ultra-sonografia e/ou Rxde abdome. Resultados: 69 (44,5%) pacientes apresentaram HC (333±118 mg/dia) e 86 (55,5%) outros distúrbios (NH). Não houve diferenças entre osgrupos na média de idade (40,1 ± 10,8 nos HC vs. 43 ± 13,9 anos NH), proporção homem/mulher, peso e índice de massa corporal (26,14 ± 4,58 HC vs.25,58 ± 5,16 NH). A pressão arterial foi semelhante nos dois grupos. História familiar de cálculo foi maior no grupo NH (44 NH vs. 25 no grupo HC, p<0,019). Os pacientes com HC relataram mais crises álgicas (326 HC vs. 282 NH, p<0,043) e apresentaram proporção maior de cálculos com diâmetro superior a 10 mm. A excreção de sódio foi maior no grupo HC (228,7±79 vs. 158,5±70,1 NH, p<0,0002). Conclusão: A HC esteve presente em 44,5% dos pacientes.Associou-se com maior número de crises álgicas e com cálculos maiores que os do grupo NH. A excreção de sódio foi maior no grupo HC e correlacionousecom a calciúria.


Introduction: Hypercalciuria is a common metabolic disorder associated with nephrolithiasis. However, little is known about the risk related to stoneformation. Objective: To compare demographic data and clinical course of nephrolithiasis patients with hypercalciuria (HC) and patients with others metabolic disorders (NH). Methods: We studied 155 patients with only one metabolic abnormality and with a minimum follow-up of one year. Two 24-h urine collections for volume, pH, calcium, creatinine, sodium, uric acid, citrate, and oxalate were obtained. Radiological evaluation consisted in abdominal ultrasound and/or. X-Ray. Results: Sixty-nine (44.5%) patients presented with HC (333±118 mg/day) and 86 (55.5%) with other metabolic disorders. There were no differences in age (40.1±10.8 HC vs. 43±13.9 years NH), gender, weight, and body mass index (26.14 ± 4.58 HC vs. 25.58 ± 5.16 NH). Blood pressure was similar in both groups. A familial history was present in 44 patients with NH vs. 25 in the HC group (p<0.019). HC patients reported higher frequency of both pain crisis (326 HC vs. 282 NH, p<0.043) and stones larger than 10 mm. Sodium excretion was higher in HC group (228.7±79 vs. 158.5±70.1 NH, p<0.0002). Conclusion: HC was present in 44.5% of patients. It was associated with a higher frequency of both pain crisis and larger stones than NH group. Sodium excretion was higher in HC patients and was directly correlated with urinary calcium.


Subject(s)
Humans , Male , Female , Adult , Citric Acid/analysis , Hypercalciuria/complications , Hypercalciuria/diagnosis , Nephrolithiasis/diagnosis , Nephrolithiasis/etiology , Sodium/analysis
9.
Braz. j. microbiol ; 34(1): 1-4, Jan.-Apr. 2003. tab
Article in English | LILACS | ID: lil-344555

ABSTRACT

Mutants of Aspergillus niger N402, induced by UV mutagenesis, were selected and tested for resistance or sensitivity to 5-fluorocytosine. Some mutants showed increased citric acid production, which did not correlate with the intracellular amount of protein or ammonium ion. The resistance to 5-fluorocytosine proved to be a rational approach for isolation of new mutants with improved production of citric acid. The best mutant (FR13) accumulated double the amount of citric acid produced by the parental strain.


Subject(s)
Citric Acid/analysis , Citric Acid/metabolism , Aspergillus niger , Flucytosine , In Vitro Techniques , Mutagenesis , R Factors
10.
Rev. odonto ciênc ; 18(39): 17-22, jan.-mar. 2003. tab, graf
Article in Portuguese | LILACS, BBO | ID: lil-365792

ABSTRACT

O objetivo deste trabalho foi avaliar as reações teciduais induzidas por alguns ácidos, durante a fase exsudativa do processo inflamatório. Utilizando-se do teste edemogênico, o EDTA, EGTA, ácido cítrico e soro fisiológico foram avaliados nos períodos de tempo de 12, 24 e 48 horas. A análise dos dados demostrou que não houve diferença estatisticamente significante entre o EGTA eo ácido cítrico - 12 h, entre o EGTA e soro - 24 h entre as 3 substâncias - 48 h. Notou-se diferença significante entre o EDTA e todos os demais grupos, sendo o EDTA a substância mais irritante independente do tempo analisado.


Subject(s)
Animals , Rats , Citric Acid/analysis , Edetic Acid/analysis , Egtazic Acid/analysis
11.
Salusvita ; 22(2): 17-180, 2003. tab
Article in Portuguese | LILACS | ID: lil-401268

ABSTRACT

O ácido cítrico é um ácido orgânico biológico que vem sendo utilizado na Odontologia como coadjuvante na terapia endondôntica, periodontal e restauradora. A utilização desta substância na Endodontia visa basicamente a desmineralização das paredes do conduto radicular durante o preparo biomecânico, com o intuito de remover a "smear layer", permitindo uma melhor adesão dos materiais obturadores a dentina. Sabendo da importância do dano químico que uma substância irrigadora pode causar, quando inavertidamente alcança os tecidos periodontais, o objetivo deste trabalho é avaliar in "in vivo" o potencial irritativo do ácido cítrico em diferentes concentrações, nos tempos de, 1/2, 1, 3 e 6 horas, pela técnica de exsudação de corantes vitais. Inicialmente (1/2 hora) todas as concentrações foram irritantes ao tecido conjuntivo e no decorrer do tempo, elas se diferenciam entre si. Com relação à concentração, as soluções de ácido cítrico, a 1 por cento e 10 por cento, foram menos irritantes que as demais. Houve uma semelhança de comportamento entre as soluções a 5 por cento e 25 por cento, nos períodos de tempo de 1,3 e 6h, sendo as mais irritantes. A solução menos irritante foi o ácido cítrico a 1 por cento. Os autores recomendam cautela no uso clínico deste ácido, optando sempre que possível por concentrações mais baixas...


Subject(s)
Humans , Male , Female , Citric Acid/analysis , Dental Pulp Test , Endodontics
12.
Interciencia ; 26(7): 290-295, jul. 2001. tab, graf
Article in Spanish | LILACS | ID: lil-341014

ABSTRACT

Se desarrolló un diseño multifactorial 2K con puntos centrales para evaluar el efecto de diferentes niveles de sulfito de sodio ácido L-ascórbico y ácido cítrico, disueltos en jarabes de sacarosa de diferentes concentraciones, en el pardeamiento del cambur durante 4,8 y 12 días de almacenamiento, a través de la medición del cambio neto de color (deltaE). Mediante el análisis de superficie de respuesta a partir de los datos experimentales, se estableció el uso óptimo de los inhibidores del pardeamiento. Se determinó, utilizando un nivel de confianza de 95 por ciento, que la impregnanción al vacío utilizando un jarabe con 300 ppm (p/p) de sulfito de sodio, 1 por ciento (p/p) de ácido L-ascórbico, 1 por ciento (p/p) de ácido cítrico y 30 por ciento (p/p) de sacarosa, logró minimizar el cambio neto de color de la fruta durante ocho días de almacenamiento, aún cuando la aceptación sensorial del producto para el atributo de calidad estudiado se prolonga solo hasta el cuarto día de almacenamiento


Subject(s)
Ascorbic Acid/analysis , Citric Acid/analysis , Biochemistry , Food Preservation/methods , Fruit , In Vitro Techniques , Sucrose , Zingiberales , Science , Venezuela
13.
Araçatuba; s.n; 2001. 228 p. ilus. (BR).
Thesis in Portuguese | LILACS, BBO | ID: lil-296714

ABSTRACT

O processo de reparo em alvéolos dentais infectados de ratos (Rattus novegicus, albinus, Wistar) diabéticos controlado e näo controlado foi avaliado qualitativa e quantitativamente. Para isso foram utilizados 60 animais. O Grupo I (Controle) foi submetido à inoculaçäo de soluçäo tampäo, os Grupos II e III (Diabéticos) receberam estreptozotocina (45mg/Kg), dissolvida em tampäo citrato 0,01M, sendo administradas 3 unidades/dia de insulina apenas no Grupo III (Diabético controlado). Após a verificaçäo do estado glicêmico dos animais, todos os incisivos superiores direitos foram extraídos e induziu-se a alveolite com soluçäo salina pré-reduzida e epinefrina. Os animais foram sacrificados no 3§, 7§, 14§ e 28§ dias pós-operatórios. Suas maxilas foram separadas, fixadas em formalina, descalcificadas em EDTA e incluídas em parafina. Os cortes com 6µm de espessura foram corados com Hematoxilina e eosina (H.E.) e Tricrômio de Masson. Após a análise qualitativa e quantitativa ao microscópio óptico e histometria óssea com o Software Imagelab, os resultados foram submetidos ao teste de Kruskal-Wallis. Concluiu-se que: qualitativamente o reparo alveolar do grupo diabético näo controlado foi mais retardado em relaçäo ao controle e ao diabético controlado nas últimas fases da reparaçäo sem apresentar diferenças estatisticamente significantes


Subject(s)
Animals , Rats , Diabetes Mellitus , Dry Socket , Tooth Socket , Citric Acid/administration & dosage , Citric Acid/analysis , Insulin/administration & dosage , Insulin/analysis , Streptozocin/administration & dosage , Streptozocin/analysis
14.
Rev. Asoc. Odontol. Argent ; 86(1): 50-53, ene.-feb. 1998. ilus, tab
Article in Spanish | LILACS, BBO | ID: biblio-872228

ABSTRACT

La remoción del barro dentinario, que se forma por la instrumentación de los conductos radiculares, es importante para el aumento de la permeabilidad por limpiar los orificios de los túbulos dentinarios. En este estudio "in vitro" se utilizaron 90 dientes unirradiculares de humanos, distribuidos en 3 grupos de 30, instrumentados con limas K (15-40), teniendo el hipoclorito de sodio (NaOCl) al 1 per cent como solución irrigante. En el primer grupo, se utilizó únicamente el NaOCl; en el 2º y 3º grupo, además de la solución irrigante, se anãdieron respectivamente el ácido cítrico, al 50 per cent, y el ácido etileno diaminotetracético (EDTA) como soluciones auxiliares. La penetración del colorante Rodamina B en la masa dentinaria evidenció el aumento de la permeabilidad. Los resultados analizados permitieron concluir que la solución irrigante associada con la solución auxiliar de EDTA fue, entre las probadas, aquella que presentó mejores efectos tras comprobación estadística


Subject(s)
Root Canal Irrigants/administration & dosage , Root Canal Irrigants/analysis , Root Canal Therapy/instrumentation , Citric Acid/administration & dosage , Citric Acid/analysis , Egtazic Acid/administration & dosage , Egtazic Acid/analysis , Endodontics , Smear Layer , Sodium Hypochlorite/administration & dosage , Sodium Hypochlorite/analysis
15.
Säo Paulo; s.n; 1998. 80 p. ilus, tab.
Thesis in Portuguese | LILACS, BBO | ID: lil-211230

ABSTRACT

Buscou-se verificar in vitro a remoçäo de restos pulpares e magma dentinário das paredes dos canais radiculares, quando do emprego na irrigaçäo final de substâncias químicas segundo três diferentes técnicas. No P.Q.C. utilizou-se a técnica telescópica e hipoclorito de sódio a 1 por cento. A irrigaçäo final foi de 30 ml: Grupo I - 10ml de hipoclorito de sódio a 1 por cento + 10 ml de ácido cítrico a 10 por cento + 10ml de água destilada; Grupo II - 15ml de hipoclorito de sódio a 0,5 por cento + 15 ml de EDTA-T; Grupo III - 10 ml de hipoclorito de sódio a 5 por cento + 10 ml de água oxigenada a 3 por cento + 10 ml de hipoclorito de sódio a 5 por cento. A avaliaçäo pelo MEV fez-se pela média da leitura dos túbulos dentinários visíveis segundo 3 observadores de uma fotomicrografia de cada terço...


Subject(s)
Root Canal Irrigants/analysis , Root Canal Irrigants/pharmacokinetics , Root Canal Irrigants/therapeutic use , Root Canal Therapy , Citric Acid/analysis , Citric Acid/pharmacokinetics , Citric Acid/therapeutic use , Endodontics , Microscopy, Electron, Scanning , Sodium Hypochlorite/analysis , Sodium Hypochlorite/pharmacokinetics , Sodium Hypochlorite/therapeutic use
16.
Rev. Fac. Odontol. Bauru ; 2(3): 34-9, jul. 1994.
Article in Portuguese | LILACS, BBO | ID: lil-222504

ABSTRACT

Este trabalho teve como objetivo comparar a efetividade de duas substâncias químicas, ácido cítrico (AC) pH 2,0 e cloridrato de tetraciclina (CT) a 2 por cento quando aplicadas em fragmentos dentários humanos durante 3 minutos e implantados no subcutâneo de ratos. Foram feitos 8 grupos, variando o período, 30 e 60 dias. Grupo 1: Raspagem(R)+CT; Grupo 8: Fragmento com doença periodontal (DP). De acordo com os resultados estatísticos obtidos pode-se concluir que as substâncias químicas comportaram-se de modo semelhante quando considerados os diversos parâmetros avaliados


Subject(s)
Animals , Rats , Citric Acid/analysis , Citric Acid/chemistry , Root Canal Therapy , Tetracycline/analysis , Tetracycline/chemistry , Periodontics
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