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1.
Article in English | WPRIM | ID: wpr-91222

ABSTRACT

The nitric oxide (NO) formation and intrinsic nitrosation may be involved in the possible mechanisms of liver fluke-associated carcinogenesis. We still do not know much about the responses of inducible NO synthase (iNOS) induced by Clonorchis sinensis infection. This study was conducted to explore the pathological lesions and iNOS expressions in the liver of mice with different infection intensity levels of C. sinensis. Extensive periductal inflammatory cell infiltration, bile duct hyperplasia, and fibrosis were commonly observed during the infection. The different pathological responses in liver tissues strongly correlated with the infection intensity of C. sinensis. Massive acute spotty necrosis occurred in the liver parenchyma after a severe infection. The iNOS activity in liver tissues increased, and iNOS-expressing cells with morphological differences were observed after a moderate or severe infection. The iNOS-expressing cells in liver tissues had multiple origins.


Subject(s)
Animals , Female , Humans , Mice , Clonorchiasis/enzymology , Clonorchis sinensis/physiology , Liver/enzymology , Mice, Inbred BALB C , Nitric Oxide Synthase Type II/genetics
2.
Article in English | WPRIM | ID: wpr-219737

ABSTRACT

The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.


Subject(s)
Animals , Antibodies, Helminth/metabolism , Clonorchis sinensis/physiology , Electrophoresis, Gel, Two-Dimensional/veterinary , Helminth Proteins/chemistry , Hydrogen-Ion Concentration , Immunoglobulin G/chemistry , Microfilament Proteins/chemistry
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