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1.
Rev. ADM ; 78(1): 13-21, ene.-feb- 2021. ilus, tab
Article in Spanish | LILACS | ID: biblio-1152240

ABSTRACT

Existe una creciente preocupación sobre el tema de la infección cruzada en clínicas y laboratorios dentales. El laboratorio odontológico debe seguir normas de bioseguridad que garanticen a todo el equipo de salud la prevención de estas infecciones. Los técnicos que allí laboran corren el riesgo de exponer su cara a salpicaduras, así como a rocíos de sangre y saliva. Este estudio fue diseñado para saber si los laboratorios a los que recurrimos cumplen con estas normas de bioseguridad, y qué tan confiados podemos estar de la desinfección por parte de ellos, ya que las prótesis deberían estar desinfectadas correctamente antes de colocarlas en boca (AU)


There is growing concern about the issue of cross infection in dental clinics and laboratories. The dental laboratory must follow biosafety standards that guarantee the prevention of these infections to the entire health team. The technicians who work there run the risk of exposing their face to splashes and spray of blood and saliva. This study was designed to find out if the laboratories we use comply with these biosafety standards, and how confident we can be of their disinfection by them, since the prostheses should be properly disinfected before placing them in the mouth (AU)


Subject(s)
Disinfection , Gram-Positive Bacterial Infections , Gram-Negative Bacterial Infections , Dental Prosthesis/adverse effects , Infection Control, Dental/methods , Laboratories, Dental , Colony Count, Microbial , Cross-Sectional Studies , Statistical Analysis , Analysis of Variance , Dental Offices/standards , Culture Techniques
2.
ABCS health sci ; 46: e021203, 09 fev. 2021. tab
Article in English | LILACS | ID: biblio-1147180

ABSTRACT

INTRODUCTION: The resistance of fungal species to drugs usually used in clinics is of great interest in the medical field. OBJECTIVE: To evaluate susceptibility and in vitro response of species of Trichophyton spp. to antifungal drugs of interest in clinical medicine. METHODS: 12 samples of clinical isolates from humans were used, nine of T. mentagrophytes and three of T. tonsurans. Susceptibility tests were performed according to the agar diffusion (AD) and broth microdilution (BM) methods. RESULTS: In the AD method, the species T. tonsurans presented a percentage of sensitivity of 33% in relation to amphotericin B and 66% to itraconazole, with 100% resistance to ketoconazole and fluconazole. T. mentagrophytes also showed 100% resistance to ketoconazole in this technique, with 11% sensitivity to ketoconazole, 22% to itraconazole and 22% of samples classified as sensitive dose dependent. In the MC method, the species T. tonsurans presented a sensitivity percentage of 66%, 55% and 33% in relation to ketoconazole, fluconazole and itraconazole, respectively. The T. mentagrophytes species presented sensitivity percentages of 11%, 11%, 33% and 55% for amphotericin B, itraconazole, ketoconazole and fluconazole, respectively. CONCLUSION: There was resistance in vitro of the species of T. mentagrophytes and T. tonsurans against the antifungal fluconazole and relative resistance against ketoconazole in the AD method. In BM, however, important percentages of sensitivity were observed for the two species analyzed in relation to the antifungals fluconazole and ketoconazole when compared to itraconazole and amphotericin B.


INTRODUÇÃO: A resistência de espécies fúngicas às drogas usualmente empregadas no meio clínico é motivo de grande interesse na área médica. OBJETIVO: Avaliar susceptibilidade e resposta in vitro de espécies de Trichophyton spp. a drogas antifúngicas de interesse em clínica médica. MÉTODOS: Foram utilizadas 12 amostras de isolados clínicos de humanos, sendo nove de T. mentagrophytes e três de T. tonsurans. Foram realizados testes de susceptibilidade segundo os métodos de difusão em ágar (DA) e microdiluição em caldo (MC). RESULTADOS: No método de DA, a espécie T. tonsurans apresentou percentual de sensibilidade de 33% em relação à anfotericina B e de 66% ao itraconazol, com 100% de resistência frente ao cetoconazol e ao fluconazol. A espécie T. mentagrophytes também apresentou 100% de resistência frente ao cetoconazol nesta técnica, com 11% de sensibilidade ao cetoconazol, 22% ao itraconazol e 22% das amostras classificadas como sensível dose dependente. No método de MC, a espécie T. tonsurans apresentou percentual de sensibilidade de 66%, 55% e 33% em relação ao cetoconazol, fluconazol e itraconazol, respectivamente. A espécie T. mentagrophytes apresentou percentuais de sensibilidade de 11%, 11%, 33% e 55% para anfotericina B, itraconazol, cetoconazol e fluconazol, respectivamente. CONCLUSÃO: Houve resistência in vitro das espécies do T. mentagrophytes e T. tonsurans frente ao antifúngico fluconazol e resistência relativa frente ao cetoconazol no método de DA. Na MC, no entanto, foram observados importantes percentuais de sensibilidade das duas espécies analisadas frente aos antifúngicos fluconazol e cetoconazol quando comparadas ao itraconazol e à anfotericina B.


Subject(s)
Trichophyton/drug effects , Microbial Sensitivity Tests , Drug Resistance, Fungal , Disease Susceptibility/microbiology , Antifungal Agents/pharmacology , Tinea/microbiology , Tinea/drug therapy , Colony Count, Microbial , Fluconazole/pharmacology , Amphotericin B/pharmacology , Itraconazole/pharmacology , Ketoconazole/pharmacology
3.
Arq. odontol ; 57: 141-148, jan.-dez. 2021. ilus, tab
Article in English | LILACS, BBO | ID: biblio-1343550

ABSTRACT

Aim: To evaluate the effect of three natural antifungal agents combined with routine denture care on the treatment of DS, using a quantitative mycological culture analysis. Methods: Thirty denture wearers with denture stomatitis DS were treated using five substances: sterile distilled water (G1), nystatin oral suspension (G2), 20% alcoholic extract propolis (G3), Punica granatumLinné gel (G4), and Uncaria tomentosa gel (G5). The substances were used 3 times a day for 14 days. Quantitative mycological culture analysis of samples collected from the palatal mucosa was performed at three stages: before treatment (T0), after 14 days of treatment (T1), and 30 days after treatment completion (T2). Data were evaluated using Kruskal-Wallis and Friedman tests (p < 0.05). Results: Palatal mucosa intragroup analysis showed a significant reduction of Candida CFU/mL values for all groups at T1 compared to T0 (p < 0.05). However, they did not present statistical differences when comparing T1 and T2 (p > 0.05). The intergroup analysis demonstrated that there are no statistical differences, regardless of the evaluation time (p > 0.05). Conclusion:The natural products tested showed a satisfactory result on DS treatment, which proved to be equivalent to conventional topical therapy with nystatin and to treatment using only regular oral hygiene procedures.


Objetivo: Avaliar o efeito de três antifúngicos naturais combinados com o cuidado rotineiro com próteses dentárias no tratamento da EP, por meio de uma análise quantitativa de cultura micológica. Métodos: Trinta usuários de próteses dentárias com EP foram tratados com cinco substâncias: água destilada estéril (G1), suspensão oral de nistatina (G2), extrato alcoólico de própolis 20% (G3), gel Punica granatum L. (G4) e gel Uncaria tomentosa (G5). As substâncias foram utilizadas 3 vezes ao dia durante 14 dias. A análise micológica quantitativa das amostras coletadas da mucosa palatina foi realizada em três etapas: antes do tratamento (T0), após 14 dias do tratamento (T1) e 30 dias após o término do tratamento (T2). Os dados foram avaliados pelos testes de Kruskal-Wallis e Friedman (p < 0,05). Resultados: A análise intragrupo da mucosa palatina mostrou uma redução significativa dos valores de Candida UFC/mL para todos os grupos em T1 em comparação com T0 (p < 0,05). No entanto, não apresentaram diferenças estatísticas na comparação de T1 e T2 (p > 0,05). A análise intergrupos demonstrou que não há diferenças estatísticas, independentemente do tempo de avaliação (p > 0,05). Conclusão: Os produtos naturais testados apresentaram resultado satisfatório no tratamento da EP, sendo equivalente à terapia tópica convencional com nistatina e ao tratamento apenas com procedimentos rotineiros de higiene bucal.


Subject(s)
Stomatitis, Denture , Biological Products , Candida albicans , Colony Count, Microbial , Antifungal Agents , Propolis , Distilled Water , Nystatin
4.
Rev. Fundac. Juan Jose Carraro ; 24(44): 40-47, 2021. ilus, tab
Article in Spanish | LILACS | ID: biblio-1223492

ABSTRACT

Las enfermedades del periodonto tienen una etiopatogenia compleja y puede considerarse multifactorial. El factor etiológico esencial en la patología inflamatoria periodontal es la biopelícula dental y cuando el desequilibrio entre el huésped y los microorganismos cambia la complejidad de la flora. Ciertas bacterias como Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella loescheii, Fusobacterium nucleatum, Tannerrella forsythia, Campylobacter rectus, Eikenella corrodens y Treponema spp., han sido comúnmente relacionadas con la periodontitis crónica y son consideradas como indicadores de riesgo para la progresión de dicha enfermedad. El objetivo de este trabajo fue establecer la prevalencia de Prevotella spp y Porphyromona spp en los distintos estadios de periodontitis crónicas. Material y métodos: Se estudiaron 48 pacientes sistémicamente saludables con diagnóstico de periodontitis crónica. Se completó el consentimiento informado, se realizó historia clínica y examen periodontal. El estado periodontal se clasificó en distintos grados de severidad: leve, moderada y severa. Se tomaron muestras de dos sitios con mayor profundidad de sondaje con conos de papel absorbente estériles y se transportaron en un medio prerreducido. Para el aislamiento de Prevotella spp se utilizó agar Brucella más sangre ovina al 5%, hemina, vitamina K al que se agregaron vancomicina y kanamicina; Porphyromonas sp se aisló en el mismo medio con el agregado de bacitracina y colistina. Se sembraron 10 µl de muestra entera y las placas fueron incubadas en jarras de anaerobiosis por 5 a 7 días a 37ºC. Resultados: los distintos grados de periodontitis correspondieron a un 17% periodontits leve, 57% moderada y 26% severa. En el total de pacientes se determinó la presencia de Prevotella spp en el 54% de los casos y un 12,5% de Porphyromona spp. Conclusión: De los pacientes estudiados con periodontits crónica, un 52% correspondió al sexo masculino, un 57% de los casos correspondieron a periodontitis moderada. Se aisló Prevotella sp en todos los estadios de periodontitis crónica y Porphyromonas sp sólo en periodontitis severas (AU)


Periodontal diseases have a complex etiopathogenesis and can be considered multifactorial. The essential etiological factor in periodontal inflammatory pathology is the dental biofilm and when the imbalance between the host and the microorganisms changes the complexity of the flora. Certain bacteria such as Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Prevotella loescheii, Fusobacterium nucleatum, Tannerrella forsythia, Campylobacter rectus, Eikenella corrodens and Treponema spp., Have been commonly related to chronic periodontitis and are considered as risk indicators for the progression of said disease. The objective of this work was to establish the prevalence of Prevotella spp and Porphyromonas spp in the different stages of chronic periodontitis. Forty eight systemically healthy patients diagnosed with chronic periodontitis were studied. Informed consent was completed, a medical history and periodontal examination was carried out. The periodontal state was classified into different degrees of severity: mild, moderate and severe. Samples were taken from two sites with greater depth of probing with sterile absorbent paper cones and transported in a prereduced medium. For the isolation of Prevotella spp, Brucella agar plus 5% sheep blood, hemin, vitamin K to which vancomycin and kanamycin were added. For Porphyromonas spp, the same medium was used and bacitracin and colistin were added. 10 µl of the whole sample was seeded and the plates were incubated in anaerobic jars for 5 to 7 days at 37 ° C. Different degrees of periodontitis corresponded to 17% mild periodontitis, 57% moderate and 26% severe. In the total number of patients, the presence of Prevotella spp was determined in 54% of the cases and 12.5% of Porphyromona spp. Of the patients studied with chronic periodontitis, 52% corresponded to the male sex, 57% of the cases corresponded to moderate periodontitis. Prevotella spp was isolated in all stages of chronic periodontitis and Porphyromonas sp only in severe periodontitis (AU)


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Porphyromonas gingivalis/isolation & purification , Prevotella intermedia/isolation & purification , Chronic Periodontitis/microbiology , Colony Count, Microbial , Risk Factors , Culture Media , Dental Plaque/microbiology , Age and Sex Distribution
5.
Rev. cuba. invest. bioméd ; 39(4): e687, oct.-dic. 2020. tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1156457

ABSTRACT

Objetivo: Determinar las propiedades antimicrobianas de la incorporación de nanopartículas de óxido de zinc y cobre en un adhesivo de grabado y lavado total sobre Streptococcus mutans en pacientes con restauraciones de resina compuesta confeccionadas con adhesivo cargado. Métodos: Este estudio clínico randomizado, participaron 25 pacientes, de ambos sexos, pertenecientes al posgrado de Ortodoncia de la Facultad de Odontología de la Universidad de Chile, en los cuales se confirmó presencia de Streptococcus mutans en saliva. Se confeccionaron restauraciones de resina compuesta oclusales, en premolares superiores con indicación de exodoncia por el tratamiento de ortodoncia, con adhesivo cargado (cuya composición es 5 / 0.2 por ciento ZnO y Cu respectivamente) y control (sin presencia de nanopartículas en su composición), según el listado de aleatorización. Se tomaron muestras microbiológicas en tres tiempos con la Técnica de la cubeta (antes, 1 semana y 4 semanas posterior a la confección de las restauraciones). Se obtuvieron, aislaron e identificaron colonias de Streptococcus mutans a partir de las muestras obtenidas. Los datos fueron analizados por el test de Mann Whittney. Resultados: El promedio del recuento de UFC (unidades formadoras de colonias) de Streptococcus mutans en el grupo experimental fue mayor posterior a la confección de las restauraciones de resina compuesta.Los resultados de la identificación molecular por PCR demuestran la presencia de Streptococcus mutans en 20 de 25 muestras. Conclusiones: No existen diferencias en el recuento de Streptococcus mutans antes y después de la aplicación del adhesivo sobre las restauraciones de resina compuesta(AU)


Objective: Determine the antimicrobial properties of the incorporation of copper and zinc oxide nanoparticles into a total rinse and etch adhesive against Streptococcus mutans in patients with composite resin restorations made with loaded adhesive. Methods: A randomized clinical study was conducted of 25 patients of both sexes from the orthodontics graduate course taught at the Dental School of the University of Chile, in whom the presence of Streptococcus mutans was confirmed in saliva. Occlusal composite resin restorations were performed in upper premolars with exodontia indicated as part of the orthodontic treatment, using loaded adhesive (composition 5 / 0.2 percent ZnO and Cu, respectively) and control (without nanoparticles in its composition), according to the randomization list. Microbiological samples were taken at three moments applying the tray technique (before, 1 week after and 4 weeks after the restorations). Streptococcus mutans were obtained, isolated and identified from the samples taken. Data analysis was based on the Mann-Whitney test. Results: Mean Streptococcus mutans CFU count in the experimental group was higher after the composite resin restorations were made. Results of PCR molecular identification show the presence of Streptococcus mutans in 20 of 25 samples. Conclusions: No differences were found in the Streptococcus mutans count before and after application of the adhesive over the composite resin restorations(AU)


Subject(s)
Humans , Male , Female , Streptococcus mutans/growth & development , Colony Count, Microbial , Dental Cements/therapeutic use , Metal Nanoparticles/therapeutic use , Surgery, Oral/methods , Epidemiology, Experimental
6.
Rev. cuba. invest. bioméd ; 39(4): e683, oct.-dic. 2020. tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1156456

ABSTRACT

Objetivo: Determinar las propiedades antimicrobianas de la incorporación de nanopartículas de óxido de zinc y cobre en un adhesivo de grabado y lavado total sobre Streptococcus mutans en pacientes con restauraciones de resina compuesta confeccionadas con adhesivo cargado. Métodos: Estudio experimental, randomizado, la muestra estuvo conformada por 25 pacientes, de ambos sexos, pertenecientes al posgrado de Ortodoncia de la Facultad de Odontología de la Universidad de Chile, en los cuales se confirmó presencia de Streptococcus mutans en saliva. Se confeccionaron restauraciones de resina compuesta oclusales, en premolares superiores con indicación de exodoncia por el tratamiento de ortodoncia, con adhesivo cargado (cuya composición fue 5/0,2 por ciento ZnO y Cu, respectivamente) y control (sin presencia de nanopartículas en su composición), según el listado de aleatorización. Se tomaron muestras microbiológicas en tres tiempos con la técnica de la cubeta (antes, 1 semana y 4 semanas posterior a la confección de las restauraciones). Se obtuvieron, aislaron e identificaron colonias de Streptococcus mutans a partir de las muestras obtenidas. Se usó el test de Mann-Whitney mediante el paquete estadístico SPSS v.21 Resultados: El promedio del recuento de UFC de Streptococcus mutans en el grupo experimental fue mayor posterior a la confección de las restauraciones de resina compuesta. Los resultados de la identificación molecular por PCR demuestran la presencia de Streptococcus mutans en 20 de 25 muestras. Conclusiones: No existen diferencias en el recuento de Streptococcus mutans antes y después de la aplicación del adhesivo sobre las restauraciones de resina compuesta(AU)


Objective: To determine the antimicrobial properties of the incorporation of zinc and copper oxide nanoparticles in an etching and total wash adhesive on Streptococcus mutans in patients with composite resin restorations made with loaded adhesive. Methods: Experimental and randomized trial, the sample were 25 patients, of both sexes, belonging to the FOUCH Orthodontic postgraduate program, in whom the presence of Streptococcus mutans in saliva was confirmed. Occlusal composite resin restorations were made in upper premolars with indication of extraction by orthodontic treatment, with loaded adhesive (whose composition is 5 / 0.2% ZnO and Cu respectively) and control (without the presence of nanoparticles in their composition), according to the scrambling listing. Microbiological samples were taken in three stages with the cuvette technique (before, 1 week and 4 weeks after the restoration was made). Colonies of Streptococcus mutans were obtained, isolated and identified from the samples obtained. The statistical analysis used the SPSS v.21 software, the data was analyzed by Mann Whitney test Results: The average CFU count of Streptococcus mutans in the experimental group (adhesive modified with zinc oxide and copper nanoparticles) was higher after the fabrication of composite resin restorations. The results of molecular identification by PCR demonstrate the presence of Streptococcus mutans in 20 of 25 samples. Conclusions: There are no differences in the count of Streptococcus mutans before and after the application of the adhesive on the composite resin restorations(AU)


Subject(s)
Humans , Male , Female , Streptococcus mutans/growth & development , Colony Count, Microbial/methods , Dental Cements/therapeutic use , Metal Nanoparticles/standards
7.
Article in Spanish | LILACS, BNUY, UY-BNMED | ID: biblio-1142102

ABSTRACT

En las fracturas abierta una de las complicaciones más temidas por los cirujanos ortopédicos es la infección de partes blandas y ósea. A pesar de múltiples investigaciones, las pautas de manejo terapéutico para las fracturas abiertas continúan en constante cambio. El objetivo principal de nuestro trabajo fue realizar una actualización sobre la prevención de la infección en las fracturas abiertas a través de una búsqueda bibliográfica poniendo especial énfasis en la utilidad de los cultivos (recuento microbiano) de heridas en el perioperatorio de estas fracturas.


In open fractures, one of the most feared complications by orthopedic surgeons is soft tissue and bone infection. Despite multiple investigations, therapeutic management guidelines for open fractures, is constantly changing. The main objective of our work was to make an update on the prevention of infection in open fractures through a bibliographic search, with special emphasis on the usefulness of cultures (microbial count) of wounds in the perioperative period of these fractures.


Nas fraturas abertas, uma das complicações mais temidas pelos cirurgiões ortopédicos é a infecção de tecidos moles e ossos. Apesar de várias investigações, as diretrizes de tratamento terapêutico para fraturas expostas estão mudando constantemente. O principal objetivo do nosso trabalho foi atualizar a prevenção de infecção em fraturas expostas por meio de uma pesquisa bibliográfica, com ênfase especial na utilidade das culturas (contagem microbiana) de feridas no período perioperatório dessas fraturas.


Subject(s)
Humans , Wound Infection/microbiology , Wound Infection/prevention & control , Fractures, Open/microbiology , Colony Count, Microbial , Predictive Value of Tests , Perioperative Period , Fractures, Open/drug therapy , Anti-Bacterial Agents/therapeutic use
8.
Rev. Asoc. Odontol. Argent ; 108(2): 46-51, mayo-ago. 2020. tab
Article in Spanish | LILACS | ID: biblio-1121108

ABSTRACT

Objetivos: Comparar ex vivo la eficacia del instrumento XP-endo Finisher y del sistema EndoActivator en la reducción/eliminación del biofilm microbiano en conductos radiculares infectados. Materiales y métodos: Se utilizaron 23 premolares inferiores humanos extraídos cuya longitud fue estandarizada en 17 mm. Todos los conductos se prepararon con el sistema WaveOne Gold Medium (#35.06). Los dientes se esterilizaron, se inocularon con Enterococcus faecalis y se separaron en dos grupos experimentales de 10 piezas cada uno. De los 3 dientes remanentes, 1 fue utilizado como control positivo y 2, como controles negativos. En el grupo 1, las soluciones irrigantes se agitaron con XP-endo Finisher. En el grupo 2, se utilizó EndoActivator. Se tomaron muestras antes de la contaminación, luego de esta y después de la agitación de los irrigantes mediante conos de papel estériles. La carga microbiana fue sembrada en agar sangre y los conos se cultivaron en caldo tripteína de soja. La remoción de la carga microbiana se determinó por la presencia o ausencia de turbiedad del medio. Las unidades formadoras de colonias (UFC) remanentes se cuantificaron y los resultados se categorizaron como R1 (≤10 UFC) o R2 (>10 UFC). Los datos fueron analizados mediante la prueba de Fisher. Resultados: No hubo diferencias significativas entre XP-endo Finisher y EndoActivator (P>0,05). El número de usos no influyó sobre la capacidad operativa de ambos instrumentos (AU)


Aim: To compare ex vivo the effectiveness of the XP-endo Finisher and the EndoActivator in biofilm reduction/ removal from infected root canals. Materials and methods: Twenty three extracted human single-rooted lower premolars were selected and standardised to 17 mm in length. All the canals were prepared with WaveOne Gold Medium reciprocating files (#35.06). The teeth were autoclaved and inoculated with Enterococcus faecalis. The infected teeth were then assigned to 2 experimental groups of 10 teeth each according to the final irrigation/agitation protocol. Of the three remaining teeth, one was used as a positive control, and the other two were used as negative controls. In Group 1 the irrigating solutions were agitated with XP-endo Finisher while in Group 2 the EndoActivator was used. All root canals were sampled before and after contamination, and again after irrigant agitation with sterile paper points. The microbial load was spread on blood agar plates and the paper points were cultured in sterile trypticase soy broth. The removal of the microbial load was determined by visual observation of the turbidity of the media and by quantification of the number of colony-forming units (UFC). The results were categorized as R1 (≤10 UFC) or R2 (>10 UFC). Data were analysed by the Fisher's exact test at P<0.05. Results: No significant differences was found between XP-endo Finisher and EndoActivator (P>0.05) regarding their effectiveness in the reduction/removal of the microbial biofilm. The number of uses of both instruments did not affect their operative performance (AU) Conclusion: XPF and EA were both equally effective for microbial biofilm reduction/removal from ex vivo infected root canals (AU)


Subject(s)
Root Canal Irrigants/chemistry , Dental High-Speed Equipment , Biofilms , Dental Instruments , Dental Pulp Cavity/microbiology , In Vitro Techniques , Colony Count, Microbial/methods , Efficacy , Statistical Analysis , Enterococcus faecalis/isolation & purification , Culture Media
9.
J. appl. oral sci ; 28: e20190100, 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1056594

ABSTRACT

Abstract Objective: This clinical study sought to evaluate the effectiveness of passive ultrasonic activation (PUA) in eliminating microorganisms in primary endodontic infection (PEI) after instrumentation of root canals using microbiological culture and checkerboard DNA-DNA hybridization. Methodology: Twenty root canals with PEI and apical periodontitis were selected. The root canals were instrumented and then randomly divided into 2 groups, according to the irrigation method: PUA and conventional needle irrigation (CNI). Microbiological samples were collected before instrumentation (S1), after instrumentation (S2) and after irrigation with 17% EDTA (S3). The samples were subjected to anaerobic culture technique and checkerboard DNA-DNA hybridization analysis. Results: A statistically significant difference was found between CNI (23.56%) and PUA (98.37%) regarding the median percentage values for culturable bacteria reduction (p<0.05). In the initial samples, the most frequently detected species was S. constellatus (50%), and after root canal treatment was E. faecalis (50%). Conclusion: Both treatments significantly decreased the number of bacterial species compared with the initial sample. However, no statistical difference in the total microbial load between PUA and CNI groups was detected. The number of cultivable anaerobic bacteria reduced significantly using PUA, and the bacterial composition and number of bacterial species after using either CNI or PUA was similar.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Periapical Periodontitis/therapy , Root Canal Therapy/instrumentation , Ultrasonic Therapy/instrumentation , Dental Pulp Cavity/microbiology , Root Canal Irrigants/therapeutic use , Root Canal Therapy/methods , Sodium Hypochlorite/therapeutic use , Bacteria/isolation & purification , Ultrasonic Therapy/methods , Colony Count, Microbial , DNA Probes , Linear Models , Analysis of Variance , Treatment Outcome , Cone-Beam Computed Tomography , Bacterial Load , Therapeutic Irrigation/instrumentation , Therapeutic Irrigation/methods
10.
J. appl. oral sci ; 28: e20190516, 2020. graf
Article in English | LILACS, BBO | ID: biblio-1090775

ABSTRACT

Abstract This study investigated the effect of a calcium hydroxide (CH) paste (CleaniCal®) containing N-2-methyl pyrrolidone (NMP) as a vehicle on Enterococcus faecalis (E. faecalis) biofilms compared with other products containing saline (Calasept Plus™) or propylene glycol (PG) (Calcipex II®). Methodology Standardized bovine root canal specimens were used. The antibacterial effects were measured by colony-forming unit counting. The thickness of bacterial microcolonies and exopolysaccharides was assessed using confocal laser scanning microscopy. Morphological features of the biofilms were observed using field-emission scanning electron microscopy (FE-SEM). Bovine tooth blocks covered with nail polish were immersed into the vehicles and dispelling was observed. The data were analyzed using one-way analysis of variance and Tukey tests (p<0.05). Results CleaniCal® showed the highest antibacterial activity, followed by Calcipex II® (p<0.05). Moreover, NMP showed a higher antibacterial effect compared with PG (p<0.05). The thickness of bacteria and EPS in the CleaniCal® group was significantly lower than that of other materials tested (p<0.05). FE-SEM images showed the specimens treated with Calasept Plus™ were covered with biofilms, whereas the specimens treated with other medicaments were not. Notably, the specimen treated with CleaniCal® was cleaner than the one treated with Calcipex II®. Furthermore, the nail polish on the bovine tooth block immersed in NMP was completely dispelled. Conclusions CleaniCal® performed better than Calasept Plus™ and Calcipex II® in the removal efficacy of E. faecalis biofilms. The results suggest the effect might be due to the potent dissolving effect of NMP on organic substances.


Subject(s)
Animals , Cattle , Pyrrolidinones/pharmacology , Root Canal Irrigants/pharmacology , Calcium Hydroxide/pharmacology , Enterococcus faecalis/drug effects , Biofilms/drug effects , Anti-Bacterial Agents/pharmacology , Potassium Chloride/pharmacology , Potassium Chloride/chemistry , Pyrrolidinones/chemistry , Root Canal Irrigants/chemistry , Materials Testing , Calcium Chloride/pharmacology , Calcium Chloride/chemistry , Calcium Hydroxide/chemistry , Microscopy, Electron, Scanning , Sodium Chloride/pharmacology , Sodium Chloride/chemistry , Colony Count, Microbial , Reproducibility of Results , Analysis of Variance , Sodium Bicarbonate/pharmacology , Sodium Bicarbonate/chemistry , Statistics, Nonparametric , Microscopy, Confocal , Drug Combinations
11.
An. bras. dermatol ; 94(5): 527-531, Sept.-Oct. 2019. tab
Article in English | LILACS | ID: biblio-1054860

ABSTRACT

Abstract Background Malassezia, a skin saprophyte, is frequently isolated from patients with seborrheic dermatitis, which is one of the most common dermatoses in HIV-infected patients. Its role in pathophysiology has not been defined. Objective To determine whether patients living with HIV and seborrheic dermatitis have more Malassezia than those without seborrheic dermatitis. Method This is an descriptive, observational, prospective cross-sectional study to which all adult patients living with HIV that attend the infectious disease outpatient clinic at the Dr. Manuel Gea González General Hospital were invited. Patients presenting with scale and erythema were included in Group 1, while patients without erythema were included in Group 2. Samples were taken from all patients for smear and culture. Results Thirty patients were included in each group. All patients with seborrheic dermatitis had a positive smear, with varying amounts of yeasts. In the control group, 36.7% of patients had a negative smear. The results are statistically significant, as well as the number of colonies in the cultures.Study limitations The study used a small sample size and the subspecies were not identified. Conclusions Patients with clinical manifestations of seborrheic dermatitis have larger amounts of Malassezia. Further studies need to be performed to analyze if the greater amount is related to imbalances in the microbiota of the skin.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Aged , Young Adult , HIV Infections/microbiology , Dermatitis, Seborrheic/microbiology , Malassezia/isolation & purification , Skin/microbiology , Colony Count, Microbial , Cross-Sectional Studies , Prospective Studies , Sex Distribution , CD4 Lymphocyte Count
12.
Rev. ADM ; 76(5): 261-266, sept.-oct. 2019. ilus, tab
Article in Spanish | LILACS | ID: biblio-1052989

ABSTRACT

Los profesionales de la salud están expuestos a una gran variedad de microorganismos desde esporas, bacterias, hongos, virus y protozoarios que pueden encontrarse en la sangre y/o saliva de los pacientes. Cualquiera de estos microorganismos puede causar una enfermedad infectocontagiosa a través de pinchazos y/o salpicaduras producidas por el aerosol durante la práctica dental. Objetivo: Determinar la presencia bacteriana en las piezas de alta velocidad utilizadas en la práctica clínica. Material y métodos: Es un estudio experimental, observacional y transversal en el que se evaluó la contaminación de 30 piezas de alta velocidad utilizadas en la práctica clínica. Previo al estudio se efectuó una estandarización obteniendo una kappa del 0.85. Se realizó una base de datos en el programa SPSS versión 22, con el que se llevó a cabo el análisis descriptivo para determinar medidas de tendencia central. Resultados: 73.3% de las muestras analizadas tuvieron crecimiento bacteriano, entre las bacterias que se encontraron resultó que 54.5% de ellas fueron bacterias Gram positivas y el resto Gram negativas. La bacteria con mayor presencia en la muestra fue el Bacillus en 45.5% seguida del Streptococcus en 27.3%, el restante 27.2% fue Staphylococcus, Coccus y Streptobacillus. Conclusiones: El uso correcto de las piezas de alta, así como su desinfección en la consulta dental es de suma importancia, ya que nos ayudan a evitar contaminaciones cruzadas y a prevenir que dentro del área de trabajo se formen focos de infección (AU)


Health professionals are exposed to a wide variety of microorganisms from spores, bacteria, fungi, viruses and protozoa that can be found in the blood and/or saliva of patients. Any of these microorganisms can cause an infectious disease through punctures and / or splashes produced by the aerosol during dental practice.1,2 Objective: To determine the bacterial presence in the high-speed pieces used in clinical practice. Material and methods: It are an experimental, observational and transversal study; where the contamination of 30 high-speed pieces used in clinical practice was evaluated. Prior to the study, a standardization was made obtaining a kappa of 0.85. A database was made in the program SPSS version 22, with which the descriptive analysis was carried out to determine measures of central tendency. Results: 73.3% of the analyzed samples showed bacterial growth, among the bacteria that were found, 54.5% of them were gram-positive bacteria and the rest were gram-negative. The bacterium with the highest presence in the sample was for Bacillus in 45.5% followed by Streptococcus in 27.3%, the remaining 27.2% was for Staphylococcus, Coccus and Streptobacillus. Conclusions: The correct use of the discharging parts, as well as their disinfection in the dental practice is of the utmost importance as they help us to avoid cross contamination and to prevent foci of infection from forming within the work area (AU)


Subject(s)
Dental High-Speed Equipment/microbiology , Biological Contamination , Infection Control, Dental/methods , Colony Count, Microbial , Cross-Sectional Studies , Statistical Analysis , Culture Media , Dental Clinics/standards , Observational Study
13.
Int. j. odontostomatol. (Print) ; 13(3): 357-362, set. 2019. tab
Article in English | LILACS | ID: biblio-1012436

ABSTRACT

ABSTRACT: The contamination of the dental units' waterlines is a reality, which can develop individual and collective disorders. The aim of this study was to evaluate the prevalence and resistance profile of bacteria on the internal surfaces of waterlines in a dental clinic from a Dentistry school of a Brazilian university. The design was an exploratory, descriptive study with quantitative and qualitative approach. Samples (n=4) were collected for analysis at different points: the portion closest to the water reservoir of the chair, and the portion closest to the triple syringe. After collection the samples were cultured in BHI medium in an oven for 24-48 h at 37 °C. For the quantitative analysis 1 ml of each sample was used for serial dilution up to the dilution value seven. The colonies were counted after pour plate and the results expressed in UFC/cm2. The qualitative analysis was initiated with the cultivation of Agar Blood, EMB-Levine and Cetrimide Agar for 24 h, and the identification of bacteria was performed by microscopic analyses. The resistance profile was verified by classical antibiogram. The internal surfaces of unit waterlines units exhibited a mean of 2.44 x 109 CFU / cm2. Bacillus subtilis and Pseudomonas aeruginosa were identified. The resistance profile of Pseudomonas aeruginosa indicated sensitivity to all tested antibiotics. A large number of microorganisms was quantified from biofilm accumulated in the dental units' waterlines. However, they were not resistant to classic antibiogram. Better management and application of decontamination protocols for waterlines need to be applied since opportunistic infections may be associated with Pseudomonas aeruginosa.


RESUMEN: La contaminación de líneas de agua en las unidades dentarias es una realidad, generando enfermedades individuales y colectivas. El objetivo de este estudio fue evaluar la prevalencia y resistencia de las bacterias en las zonas internas de las líneas de agua de la Facultad de Odontología de una universidad brasileña. El diseño del estudio fue exploratorio, descriptivo con enfoques cuantitativos y cualitativos. Las muestras para análisis (n=4) fueron selecionadas de distintos lugares: el punto más cercano al sistema de agua del sillón odontológico y el punto más cercano a la jeringa tríplice. Las muestras obtenidas fueron cultivadas en un médio BHI por 24-48 h, en un horno a 37 ºC. Para el análisis cuantitativo, se utilizó 1 ml de cada muestra para dilución hasta el valor siete. Las colonias fueran contadas y los resultados fueron expresados en UFC/cm2. El análisis cualitativo fue iniciado con el cultivo de Agar Sangre, EBM-Levine y Agar Cetrimide por 24 h y la identificación de la bacteria fue realizada por análisis microscópicos. El perfil de resistencia fue verificado por el antibiograma clásico. Las zonas internas de las unidades de líneas de agua mostraron un promédio de 2,44 x 109 UFC/cm2. Bacillus subtilis y Pseudomonas aeruginosa fueron encontrados. El perfil de resistencia de Pseudomonas aeruginosa indicó sensibilidad a todos los antibióticos testados. Un gran número de microorganismos fue cuantificado desde la biopelícula acumulada en las líneas de agua de unidades dentales. Sin embargo, no resistieron al antibiograma clásico. Se requiere una mejor gestión y aplicación de protocolos de decontaminación en las líneas de agua debido a que las infecciones oportunistas puedan estar asociadas a Pseudomonas aeruginosa.


Subject(s)
Humans , Water/adverse effects , Biofilms , Infection Control, Dental/methods , Water Microbiology , Brazil , Colony Count, Microbial , Equipment Contamination/prevention & control , Prospective Studies , Dental Equipment/microbiology , Mycobacterium/growth & development
14.
Biosci. j. (Online) ; 35(1): 126-136, jan./fev. 2019. tab, graf, ilus
Article in English | LILACS | ID: biblio-1048566

ABSTRACT

The Asian rust (Phakopsorapachyrhizi) is one of the most destructive diseases of soybean in Brazil. Despite its importance, little is known about the airborne inoculum production dynamics of pathogen throughout the soybean cycle in Brazil. The objective of this study was to assess the temporal variation of air collected P. pachyrhizi urediniospores using a Burkard 7-day-spore-trap during 2006-2007 and 2007-2008, from November to March of each soybean (MSoy-8001) growing season. The disease severity wasquantified on soybean planted in October 18, 2006 and in October 30, 2007. From November/06 through March/07 were collected over 175,000 urediniospores, and, over 131,000 from November/07 to March/08. In 06-07, most of the spores were collected from January 9, 2007 through February 1st, 2007 (±3,000 urediniospores day-1), and, in 07-08 most urediniospores was collected from February 8, 2008 throughout March 2, 2008 (±2,000 urediniospores day-1). The maximum amount of urediniospores collected in a single day in 06-07 (±15,000; 1/25/2007) was higher than in 07-08 (±12,000; 2/13/2008). In both soybean growing seasons most of the hourly urediniospores was collected from 10:00 am through 6:00 pm and the highest number from December to March, was at 3:00 pm (±16,000 in 06-07 and ±14,000 in 07-08). The disease started earlier in 2006-07 [38 days after planting (DAP)] than in 07-08 (58 DAP). The amount of collected airborne spores was positively correlated to disease severity, leaf wetness, and, precipitation along both soybean growing seasons. However, during a 24h-day evaluation, the major amount of hourly collected spores was negatively correlated to the leaf wetness and air relative humidity.


A ferrugem asiática (Phakopsorapachyrhizi) é uma das doenças mais destrutivas de soja no Brasil. Apesar de sua importância, pouco é conhecido sobre a dinâmica de distribuição de inóculo no ar do patógeno em todo o ciclo da soja no Brasil. O objetivo deste estudo foi avaliar a variação temporal deurediniósporos de P. pachyrhizi coletados do ar com uma armadilha de esporos Burkard de 7 dias de captura em 2006-2007 e 2007-2008, durante o cultivo da soja (cv. MSoy-8001) entre novembro e março. A severidade da doença foi quantificada em soja plantada em 18 de outubro de 2006 e em 30 de outubro de 2007. De novembro/06 até março/07 foram coletados mais de 175.000 urediniósporos, e, mais de 131.000 de novembro/07 a março/08. Em 06 / 07, a maioria dos esporos foram coletada de 9 de janeiro de 2007 a 1º. de fevereiro de 2007 (± 3.000 urediniósporos dia-1), e, em 07-08 a maioria dos urediniósporos foi coletada entre 8 de fevereiro de 2008 e 2 de março de 2008 (±2,000 uredosporo dia-1). A quantidade máxima de urediniósporos coletados em um único dia em 06-07 (±15,000; 25/1/2007) foi superior a 07-08 (±12,000; 13/2/2008). Em ambas as safras de soja a maioria dos urediniósporos em cada hora foi coletado entre 10:00 e 18:00 de dezembro a março e o maior número foi às 15:00 (±16,000 em 06-07 e ±14,000 em 07-08). A doença inicioumais cedo em 2006-07 [38 dias após o plantio (DAP)] do que em 07-08 (58 DAP). A quantidade de esporos no ar coletados correlacionou-se positivamente a severidade da doença, umidade foliar, e precipitação ao longo de ambos os ciclos de soja. No entanto, durante a avaliação diária, a quantidade de esporos coletados por hora foi negativamente correlacionada com umidade foliar e a umidade relativa do ar.


Subject(s)
Soybeans , Colony Count, Microbial , Phakopsora pachyrhizi
15.
Braz. oral res. (Online) ; 33: e023, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001611

ABSTRACT

Abstract: We evaluated the antifungal and antibiofilm potential of the hydroalcoholic extract of bark from Anadenanthera colubrina (vell.) Brenan, known as Angico, against Candida spp. Antifungal activity was evaluated using the microdilution technique through the Minimum Inhibitory and Fungicide Concentrations (MIC and MFC). The antibiofilm potential was tested in mature biofilms formed by Candida species and analyzed through the counting of CFU/mL and scanning electron micrograph (SEM). In vivo toxicity and therapeutic action was evaluated in the Galleria mellonella model. The treatment with the extract, in low doses, was able to reduce the growth of planktonic cells of Candida species. MIC values range between 19.5 and 39 µg/mL and MFC values range between 79 and 625 µg/mL. In addition was able to reduce the number of CFU/mL in biofilms and to cause structural alteration and cellular destruction, observed via SEM. A. colubrina showed low toxicity in the in vivo assay, having not affected the viability of the larvae at doses below 100mg/kg and high potential in the treatment of C. albicans infection. Considering its high antifungal potential, its low toxicity and potential to treatment of infections in in vivo model, A. colubrina extract is a strong candidate for development of a new agent for the treatment of oral candidiasis.


Subject(s)
Candida/drug effects , Plant Extracts/pharmacology , Biofilms/drug effects , Fabaceae/chemistry , Antifungal Agents/pharmacology , Time Factors , Microscopy, Electron, Scanning , Colony Count, Microbial , Microbial Sensitivity Tests , Nystatin/pharmacology , Reproducibility of Results , Analysis of Variance
16.
Acta odontol. latinoam ; 32(1): 36-43, 2019. tab
Article in English | LILACS | ID: biblio-1015020

ABSTRACT

The aim of this study was to evaluate the deproteinization of primary enamel by analyzing etching pattern types, with and without the application of 5% NaOCl before acid etching with 37% H3PO4. Fifteen extracted human primary molars were randomly selected for the present in vitro study; 1mm x 1mm blocks were prepared and divided into two groups (n = 21). These groups were treated as follows: Group AAcid Etching with 37% H3PO4 gel for 15 s; Group B5% NaOCl for 60 s + Acid Etching with 37% H3PO4for 15 s. The specimens were prepared for scanning electron microscopy analysis. The images were evaluated for quality types I and II etching of the enamel surface using ImageJ software. Datasets were checked for normality by KolgomorvSmirnov test and the nonparametric unpaired MannWhitney test was applied. The mean surface area of type I and II etching pattern values was 1922.314 µm2for Group A and 3840.473 µm2Group B. We conclude that deproteinization with 5% NaOCl prior to acid etching can be used to increase the area of adhesion and the quality of the etching pattern (AU)


El objetivo del estudio fue evaluar la desproteinización del esmalte primario a través de los tipos de patrones de grabado, con y sin NaOCl 5% utilizado antes del grabado ácido con H3PO4 37%. Quince dientes primarios humanos extraídos se seleccionaron al azar para el presente estudio in vitro, se prepararon bloques de 1mm x 1 mm y se dividieron en dos grupos (n = 21). Estos grupos se trataron de la siguiente manera: Grupo A: Grabado ácido con H3PO4 37% en gel durante 15 segundos; Grupo B: NaOCl 5% durante 60 segundos + Grabado ácido con H3PO4 37% durante 15 segundos. Las muestras se prepararon para el análisis de microscopía electrónica de barrido. Las imágenes obtenidas se evaluaron principalmente por la calidad de los grabados tipo I y II de la superficie del esmalte primario, utilizando el software Image J. Los datos se analizaron en cuanto a su normalidad mediante la prueba de KolgomorvSmirnov, se utilizó pruebas no paramétricas: Prueba de MannWhitney no pareada. Como resultado, se encontró que el área de superficie media de los valores de patrón de grabado de tipo I y II para el Grupo A era 1922,314 µm2 y el Grupo B era 3840,473 µm2. Finalmente, llegamos a la conclusión de que se puede usar la desproteinización con NaOCl 5% antes del grabado ácido para aumentar el área de adhesión y la calidad del patrón de grabado (AU)


Subject(s)
Humans , Male , Female , Adolescent , Adult , Periodontitis/microbiology , Culture Media , Colony Count, Microbial/methods , Cross-Sectional Studies , Statistical Analysis , Dominican Republic
17.
J. bras. pneumol ; 45(3): e20170351, 2019. tab
Article in English | LILACS | ID: biblio-1012549

ABSTRACT

ABSTRACT Objective: Home nebulizers are routinely used in the treatment of patients with cystic fibrosis (CF). This study aims to evaluate the contamination of nebulizers used for CF patients, that are chronically colonized by Pseudomonas aeruginosa, and the association of nebulizer contamination with cleaning, decontamination and drying practices. Methods: A cross-sectional, observational, multicenter study was conducted in seven CF reference centers in Brazil to obtain data from medical records, structured interviews with patients/caregivers were performed, and nebulizer's parts (interface and cup) were collected for microbiological culture. Results: overall, 77 CF patients were included. The frequency of nebulizer contamination was 71.6%. Candida spp. (52.9%), Stenotrophomonas maltophilia (11.9%), non-mucoid P. aeruginosa (4.8%), Staphylococcus aureus (4.8%) and Burkholderia cepacia complex (2.4%) were the most common isolated pathogens. The frequency of nebulizers' hygiene was 97.4%, and 70.3% of patients reported cleaning, disinfection and drying the nebulizers. The use of tap water in cleaning method and outdoor drying of the parts significantly increased (9.10 times) the chance of nebulizers' contamination. Conclusion: Despite the high frequency hygiene of the nebulizers reported, the cleaning and disinfection methods used were often inadequate. A significant proportion of nebulizers was contaminated with potentially pathogenic microorganisms for CF patients. These findings support the need to include patients/caregivers in educational programs and / or new strategies for delivering inhaled antibiotics.


RESUMO Objetivo: Nebulizadores caseiros são usados rotineiramente no tratamento de pacientes com fibrose cística (FC). Este estudo objetiva avaliar a contaminação de nebulizadores utilizados por pacientes de FC que estão cronicamente colonizados por Pseudomonas aeruginosa e a associação da contaminação do nebulizador com a higienização, esterilização e método de secagem. Métodos: Um estudo transversal, observacional, multicêntrico foi conduzido em sete centros de referência de FC no Brasil para obter dados de registros médicos; foram feitas entrevistas estruturadas com os pacientes/cuidadores e partes de nebulizadores (máscara e copo) foram coletados para cultura microbiológica. Resultados: No geral, 77 pacientes com FC foram incluídos. A frequência da contaminação do nebulizador foi de 71,6%. Candida spp. (52,9%), Stenotrophomonas maltophilia (11,9%), P. aeruginosa não mucoide (4,8%), Staphylococcus aureus (4,8%) e complexo Burkholderia cepacia (2.4%) foram os patógenos isolados mais comuns. A frequência de higienização dos nebulizadores foi de 97,4%, e 70,3% dos pacientes relata higienização, esterilização e secagem dos aparelhos. A lavagem com água da torneira e secagem das partes no tempo, em espaço aberto, aumentou significativamente (9 a 10 vezes) a chance de contaminação dos nebulizadores. Conclusões: Apesar dos relatos de frequente higienização dos nebulizadores, os métodos de limpeza e esterilização usados eram inadequados. Uma proporção significativa de nebulizadores foi contaminada com microrganismos potencialmente patogênicos para pacientes com FC. Estes resultados apoiam a necessidade de inclusão dos pacientes/cuidadores em programas educacionais e/ou novas estratégias para fornecimento de antibióticos inalatórios.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Young Adult , Pseudomonas aeruginosa/isolation & purification , Nebulizers and Vaporizers/microbiology , Equipment Contamination/statistics & numerical data , Cystic Fibrosis/therapy , Reference Values , Brazil , Colony Count, Microbial , Logistic Models , Decontamination/methods , Decontamination/statistics & numerical data , Disinfection/methods , Disinfection/statistics & numerical data , Equipment Contamination/prevention & control , Cross-Sectional Studies
18.
Rev. Soc. Bras. Med. Trop ; 52: e20180182, 2019. tab, graf
Article in English | LILACS | ID: biblio-1041508

ABSTRACT

Abstract INTRODUCTION: Administration of total parenteral nutrition (TPN) via catheters increases the risk for candidemia from Candida parapsilosis. METHODS: C. parapsilosis sensu stricto blood isolates were evaluated for ability total biomass biofilm formation and morphogenesis in presence of glucose at TPN equivalent concentrations. RESULTS: Biofilms were increased at high glucose concentrations (25-30%) compared to the control medium. Significant increase in filamentous forms was observed in cultures with 30% glucose. CONCLUSIONS: Biofilm formation by C. parapsilosis sensu stricto in hyperglycidic medium may contribute to its pathogenic potential for fungemia related to TPN catheters.


Subject(s)
Humans , Biofilms/growth & development , Candida parapsilosis/physiology , Glucose/pharmacology , Colony Count, Microbial , Parenteral Nutrition, Home Total , Biofilms/drug effects , Culture Media/chemistry
19.
Rev. Soc. Bras. Med. Trop ; 52: e20190135, 2019. graf
Article in English | LILACS | ID: biblio-1041502

ABSTRACT

Abstract INTRODUCTION: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative. METHODS: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut. RESULTS: We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected. CONCLUSIONS: The serovar, Btk176 might be a potential biocontrol agent for houseflies.


Subject(s)
Animals , Bacillus thuringiensis , Bacterial Toxins/pharmacology , Houseflies/drug effects , Insecticides/pharmacology , Larva/drug effects , Colony Count, Microbial , Pest Control, Biological/methods , Reproducibility of Results , Analysis of Variance , Microscopy, Electron, Transmission , Exotoxins
20.
J. appl. oral sci ; 27: e20180183, 2019. tab, graf
Article in English | LILACS | ID: biblio-975880

ABSTRACT

Abstract Objectives: This study investigated the physical and mechanical properties, antibacterial effect and biocompatibility of novel elastomeric temporary resin-based filling materials (TFMs) containing zinc methacrylate (ZM). Material and Methods: Experimental TFMs were prepared by mixing the zinc methacrylate with monomer, co-monomer, photoinitiator and fillers. A ZM concentration of 0 (control), 0.5% (Z0.5); 1% (Z1), 2% (Z2), or 5% (ZM5) wt% was added to the TFMs. Fermit-N (F) was used for comparison with the experimental material. Microleakage, water sorption/solubility, degree of conversion, depth of cure, ultimate tensile strength, and hardness were determined and compared. A modified direct contact test (DCT) with Enterococcus faecalis and a Streptococcus mutans' biofilm accumulation assay was carried out to evaluate the antimicrobial effect and cytotoxicity of the assay. Statistical comparisons were performed (α=5%). Results: The results showed that the physical and mechanical properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference and some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values. TFMs with ZM killed E. faecalis only after 1 h. Biofilm development of S. mutans was not affected by the inclusion of ZM in the experimental TFMs. Conclusions: The present findings suggest that the physical, mechanical and biological properties of the experimental TFMs with ZM are comparable with the properties of the commercial reference. However, some properties were improved, such as lower microleakage and water sorption, and higher ultimate tensile strength values.


Subject(s)
Animals , Cattle , Zinc/chemistry , Composite Resins/chemistry , Elastomers/chemistry , Dental Restoration, Temporary/methods , Methacrylates/chemistry , Reference Values , Solubility , Streptococcus mutans/drug effects , Tensile Strength , Time Factors , Zinc/pharmacology , Materials Testing , Colony Count, Microbial , Random Allocation , Reproducibility of Results , Enterococcus faecalis/drug effects , Composite Resins/pharmacology , Elastomers/pharmacology , Dental Leakage , Hardness Tests , Methacrylates/pharmacology , Anti-Bacterial Agents/pharmacology
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