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1.
Singap. med. j ; Singap. med. j;: 188-195, 2023.
Article in English | WPRIM | ID: wpr-969656

ABSTRACT

INTRODUCTION@#The diagnosis of Wilson disease (WD) is plagued by biochemical and clinical uncertainties. Thus, calculated parameters have been proposed. This study aimed to: (a) compare the diagnostic values of non-caeruloplasmin copper (NCC), NCC percentage (NCC%), copper-caeruloplasmin ratio (CCR) and adjusted copper in WD; and (b) derive and evaluate a discriminant function in diagnosing WD.@*METHODS@#A total of 213 subjects across all ages who were investigated for WD were recruited. WD was confirmed in 55 patients, and the rest were WD free. Based on serum copper and caeruloplasmin values, NCC, NCC%, CCR and adjusted copper were calculated for each subject. A function was derived using discriminant analysis, and the cut-off value was determined through receiver operating characteristic analysis. Classification accuracy was found by cross-tabulation.@*RESULTS@#Caeruloplasmin, total copper, NCC, NCC%, CCR, adjusted copper and discriminant function were significantly lower in WD compared to non-WD. Discriminant function showed the best diagnostic specificity (99.4%), sensitivity (98.2%) and classification accuracy (99.1%). Caeruloplasmin levels <0.14 g/L showed higher accuracy than the recommended 0.20 g/L cut-off value (97.7% vs. 87.8%). Similarly, molar NCC below the European cut-off of 1.6 umol/L showed higher accuracy than the American cut-off of 3.9 umol/L (80.3% vs. 59.6%) (P < 0.001). NCC%, mass NCC, CCR and adjusted copper showed poorer performances.@*CONCLUSION@#Discriminant function differentiates WD from non-WD with excellent specificity, sensitivity and accuracy. Performance of serum caeruloplasmin <0.14 g/L was better than that of <0.20 g/L. NCC, NCC%, CCR and adjusted copper are not helpful in diagnosing WD.


Subject(s)
Humans , Hepatolenticular Degeneration/diagnosis , Copper/analysis , Ceruloplasmin/metabolism , Repressor Proteins
2.
Zhonghua Yu Fang Yi Xue Za Zhi ; (12): 200-207, 2023.
Article in Chinese | WPRIM | ID: wpr-969867

ABSTRACT

Objective: To investigate the relationship between the levels of selenium, iron and copper in cord blood of neonates and the risk of congenital heart disease (CHD), and analyze their interaction effects. Methods: The subjects were obtained from the birth cohort in Lanzhou area established from 2010 to 2012. A baseline survey was conducted in the first trimester, and the follow-up was conducted in the second trimester, third trimester and 42 days after delivery. The umbilical vein blood was collected from newborns at delivery, and information on their birth outcomes was extracted from medical records. A nested case-control study was used to select 97 neonates with CHD newly diagnosed by echocardiography as the case group, and 194 neonates were selected as the control group by 1∶2 matching according to their mother's age, block and CHD onset time. Inductively coupled ion mass spectrometry was used to detect the concentrations of selenium, iron and copper in neonatal cord blood. The element exposure was categorized into three groups, the low, medium and high concentrations, according to the quartiles Q1 and Q3 of selenium, iron and copper concentrations in the control group. The association between cord blood selenium, iron and copper concentrations and CHD was analyzed by conditional logistic regression model using medium concentration as the reference standard. The association of their interactions with CHD was analyzed by a phase multiplication model. Results: The M (Q1, Q3) concentration of neonatal cord blood copper was 746.12 (467.48, 759.74) μg/L in the case group and 535.69 (425.21, 587.79) μg/L in the control group, with a statistically significant difference between the two groups (P<0.05). After adjustment for confounders, logistic regression models showed that the risk of CHD development was increased in neonates with either high copper in cord blood (OR=4.062, 95%CI: 2.013-8.199) or high copper combined with high iron (OR=3.226, 95%CI: 1.343-7.750). No correlation was observed between selenium and iron concentrations and the development of CHD in neonates. There was a multiplicative interaction between copper and iron in cord blood on the risk of developing CHD (OR=1.303, 95%CI: 1.056-1.608). Conclusion: There is a multiplicative interaction between iron and copper elements. The high copper and the high copper combined with high iron in umbilical cord blood are risk factors for neonatal CHD.


Subject(s)
Humans , Infant, Newborn , Copper/analysis , Selenium , Iron/analysis , Fetal Blood/chemistry , Case-Control Studies , Heart Defects, Congenital
3.
Acta amaz ; Acta amaz;51(3): 260-269, set 2021. graf, tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1455404

ABSTRACT

Cutaneous leishmaniasis is a disease caused by protozoa of the genus Leishmania and, currently, the treatment of first choice is meglumine antimoniate. However, due to its limited effectiveness and high toxicity, it is necessary to seek new active principles for leishmaniasis treatment. Metal complexes are gaining importance due to their effectiveness and low toxicity. In this context, the present study aimed to evaluate the in vitro and in vivo antileishmanial activity of the hypotoxic copper(I) complex [HB(pz)3]Cu(PCN). Four dermotropic species of Leishmania were tested with the metal complex and its effectiveness was determined through parasitic viability and infectivity rate, and cytotoxicity was determined using a redox dye (resazurin). For the in vivo tests, hamsters were infected and the lesions treated with a formulated ointment containing the complex, the effectiveness of which was assessed by measuring the diameter of the inoculum/snout location and determining the parasitic load. The results demonstrated moderate toxicity in murine macrophages and human monocytes and better efficacy in Leishmania (V.) braziliensis when compared to the other species tested, with a 50% reduction in the viability of promastigote and amastigote forms (in vitro). General data from daily topical treatment for up to 30 days showed low efficacy for reducing lesions, and no clinical and parasitological cure was observed in the experimental animals. Thus, the [HB(pz)3]Cu(PCN) complex proved to be promising in in vitro studies against L. (V.) braziliensis, and should be further tested in new formulations and new experimental treatment schemes.


A leishmaniose cutânea é uma doença causada por protozoários do gênero Leishmania e, atualmente, o tratamento de primeira escolha é o antimoniato de meglumina. Porém, devido à sua eficácia limitada e alta toxicidade, é necessário buscar novos princípios ativos para o tratamento da leishmaniose. Os complexos metálicos vêm ganhando importância devido à sua eficácia e baixa toxicidade. Nesse contexto, o presente estudo teve como objetivo avaliar a atividade leishmanicida in vitro e in vivo do complexo hipotóxico de cobre(I) [HB(pz)3]Cu(PCN). Quatro espécies dermotrópicas de Leishmania foram testadas com o complexo metálico e sua eficácia foi determinada através da viabilidade parasitária e taxa de infectividade, e a citotoxicidade foi determinada com um corante redox (resazurina). Para os testes in vivo, hamsters foram infectados e as lesões foram tratadas com uma pomada formulada contendo o complexo. A eficácia foi avaliada medindo o diâmetro do inóculo/focinho e determinando a carga parasitária. Os resultados demonstraram toxicidade moderada em macrófagos murinos e monócitos humanos e melhor eficácia em Leishmania (V.) braziliensis quando comparada às demais espécies testadas, com redução de 50% na viabilidade das formas promastigotas e amastigotas (in vitro). Os dados gerais do tratamento tópico diário por até 30 dias mostraram baixa eficácia na redução das lesões, e nenhuma cura clínica e parasitológica foi observada nos animais experimentais. Portanto, o complexo [HB(pz)3]Cu(PCN) mostrou-se promissor em estudos in vitro contra L. (V.) braziliensis, devendo ser empregado em novas formulações e novos esquemas de tratamento experimental.


Subject(s)
Copper/analysis , Leishmaniasis , In Vitro Techniques
4.
Zhongguo dangdai erke zazhi ; Zhongguo dangdai erke zazhi;(12): 445-450, 2021.
Article in Chinese | WPRIM | ID: wpr-879874

ABSTRACT

OBJECTIVE@#To study the association of serum levels of trace elements with core symptoms in children with autism spectrum disorder (ASD).@*METHODS@#From September 2018 to September 2019, an investigation was performed for 1 020 children with ASD and 1 038 healthy children matched for age and sex in the outpatient service of grade A tertiary hospitals and special education institutions in 13 cities of China. Autism Behavior Checklist (ABC), Social Responsiveness Scale (SRS), and Childhood Autism Rating Scale (CARS) were used to assess the core symptoms of the children with ASD. The inductively coupled plasma mass spectrometry was used to measure serum levels of trace elements magnesium, iron, copper, and zinc.@*RESULTS@#The children with ASD had significantly lower serum levels of magnesium, copper, and zinc than the healthy children (@*CONCLUSIONS@#The serum levels of magnesium and zinc may be associated with core symptoms in children with ASD, which requires further studies. The nutritional status of trace elements should be monitored for children with ASD in clinical practice.


Subject(s)
Child , Humans , Autism Spectrum Disorder , China , Copper/analysis , Trace Elements/analysis , Zinc
5.
Rev. chil. nutr ; 46(5): 511-517, oct. 2019. tab
Article in Spanish | LILACS | ID: biblio-1042690

ABSTRACT

RESUMEN La leche humana materna tiene una composición única para proporcionar a los recién nacidos todos los nutrientes esenciales como proteínas, grasas, hidratos de carbono, minerales, vitaminas y otras substancias fisiológicamente activas, con el fin de suplir en cantidad y calidad las necesidades calóricas y nutricionales del lactante. El objetivo fue establecer las concentraciones totales de cobre y zinc en la leche materna en sus distintas etapas: calostro, de transición y madura, determinadas espectrométricamente y la influencia del estado nutricional de madres lactantes; estudio descriptivo transversal en madres entre 18 y 35 años, sanas y no fumadoras; los resultados evidencian una disminución en las concentraciones de cobre y zinc a medida que la lactancia va evolucionando, además, el promedio de índice de masa corporal de las madres en período de lactancia reflejaron sobrepeso. Concluimos que el contenido calórico de los oligoelementos de la leche materna es esencial para el crecimiento de los niños por los beneficios nutricionales e inmunológicos que proporciona, por lo que es importante que la madre se encuentre en un estado nutricional adecuado que permita suministrar los requerimientos adecuados al niño para su crecimiento y desarrollo.


ABSTRACT Human milk has a unique composition and provides newborns with all essential nutrients such as proteins, fats, carbohydrates, minerals, vitamins and other physiologically active substances, in order to supply in quantity and quality the caloric and nutritional needs of the infant. The objective of this study was to establish the total concentration of copper and zinc in breast milk in its different stages: colostrum, transitional and mature milk, determined spectrometrically and the influence of the nutritional status of nursing mothers. We conducted a descriptive cross-sectional study in healthy non-smoking mothers between 18 and 35 years of age. Lower concentrations of copper and zinc as lactation progressed were observed. The average of body mass index of nursing mothers reflected overweight. We concludes that the caloric content of the trace elements of breast milk is essential for the growth of children because of the nutritional and immunological benefits that it provides, so it is important that the mother has an adequate nutritional state that allows to supply the adequate requirements for the child to grow and develop.


Subject(s)
Humans , Female , Infant, Newborn , Adolescent , Adult , Young Adult , Zinc/analysis , Nutritional Status , Copper/analysis , Milk, Human/chemistry , Spectrophotometry, Atomic , Cross-Sectional Studies
6.
Braz. j. microbiol ; Braz. j. microbiol;49(1): 29-37, Jan.-Mar. 2018. tab, graf
Article in English | LILACS | ID: biblio-889196

ABSTRACT

ABSTRACT Increased environmental pollution has necessitated the need for eco-friendly clean-up strategies. Filamentous fungal species from gold and gemstone mine site soils were isolated, identified and assessed for their tolerance to varied heavy metal concentrations of cadmium (Cd), copper (Cu), lead (Pb), arsenic (As) and iron (Fe). The identities of the fungal strains were determined based on the internal transcribed spacer 1 and 2 (ITS 1 and ITS 2) regions. Mycelia growth of the fungal strains were subjected to a range of (0-100 Cd), (0-1000 Cu), (0-400 Pb), (0-500 As) and (0-800 Fe) concentrations (mgkg-1) incorporated into malt extract agar (MEA) in triplicates. Fungal radial growths were recorded every three days over a 13-days' incubation period. Fungal strains were identified as Fomitopsis meliae, Trichoderma ghanense and Rhizopus microsporus. All test fungal exhibited tolerance to Cu, Pb, and Fe at all test concentrations (400-1000 mgkg-1), not differing significantly (p > 0.05) from the controls and with tolerance index >1. T. ghanense and R. microsporus demonstrated exceptional capacity for Cd and As concentrations, while showing no significant (p > 0.05) difference compared to the controls and with a tolerance index >1 at 25 mgkg-1 Cd and 125 mgkg-1 As. Remarkably, these fungal strains showed tolerance to metal concentrations exceeding globally permissible limits for contaminated soils. It is envisaged that this metal tolerance trait exhibited by these fungal strains may indicate their potentials as effective agents for bioremediative clean-up of heavy metal polluted environments.


Subject(s)
Fungi/isolation & purification , Fungi/metabolism , Metals, Heavy/metabolism , Soil Pollutants/metabolism , Cadmium/analysis , Cadmium/metabolism , Copper/analysis , Copper/metabolism , Fungi/classification , Fungi/genetics , Gold/analysis , Gold/metabolism , Metals, Heavy/analysis , Mining , Phylogeny , Soil Pollutants/analysis
7.
Braz. j. biol ; Braz. j. biol;78(1): 133-139, Feb. 2018. graf
Article in English | LILACS | ID: biblio-888829

ABSTRACT

Abstract Aquatic macrophytes have properties and mechanisms which are useful for the removal of substances in solution, commonly used in phytoremediation processes in aquatic environments. This study evaluated the performance of copper (Cu) accumulation by Salvinia biloba Raddi (Salviniaceae) in different metal concentrations (1, 3 and 5 µg mL-1), as well as the control treatment, measured at intervals of 0, 7 and 14 days under laboratory conditions, with control as to pH and luminosity. After the experiment, the S. biloba biomass was washed, kiln dried, crushed and subjected to the process of digestion, and subsequently the accumulated copper content was determined by atomic absorption spectroscopy. The results showed that S. biloba is apt at accumulating copper, varying significantly between different treatments and days of exposure to the contaminant, as well as its interaction (treatment × days). The highest accumulation values were observed in treatment with 5 µg mL-1, which at 14 days, with 11,861 µg g-1 of copper. We observed symptoms of toxicity and mortality in plants, probably indicating the effect of copper on the species when at high levels. Salvinia biloba is an efficient species in the removal of copper in solution, its recommendation as a remediating agent in aquatic ecosystems being possible.


Resumo Macrófitas aquáticas apresentam propriedades e mecanismos úteis à remoção de substâncias presentes em solução, comumente, utilizadas em processos de fitorremediação de ambientes aquáticos. Este estudo avaliou o desempenho de acumulação de cobre (Cu) por Salvinia biloba Raddi (Salviniaceae) em diferentes concentrações do metal (1, 3 e 5 µg mL-1), além do tratamento controle, avaliados em intervalos de 0, 7 e 14 dias, sob condições laboratoriais, com controle de pH e luminosidade. Após o experimento a biomassa de S. biloba, foi lavada, seca em estufa, triturada e submetida ao processo de digestão, sendo, posteriormente, o teor de cobre acumulado determinado por espectroscopia de absorção atômica. Os resultados evidenciaram que S. biloba é hábil em acumular cobre em sua biomassa, variando significativamente entre os diferentes tratamentos e dias de exposição ao contaminante, bem como por sua interação (tratamentos × dias). Os maiores teores de acúmulo foram observados no tratamento com 5 µg mL-1, que aos 14 dias apresentou até 11.861 µg g-1 de Cu. Observamos sintomas de toxicidade e mortalidade nas plantas, evidenciando, provavelmente, o efeito do cobre sobre a espécie quando em elevados níveis. Salvinia biloba é uma espécie eficiente na remoção do cobre em solução, sendo possível sua recomendação como agente de remediação em ecossistemas aquáticos.


Subject(s)
Biodegradation, Environmental , Copper/analysis , Copper/metabolism , Aquatic Organisms/metabolism , Aquatic Organisms/chemistry , Tracheophyta/chemistry , Water Pollutants, Chemical/analysis , Water Pollutants, Chemical/metabolism , Biomass , Tracheophyta/metabolism
8.
An. acad. bras. ciênc ; 89(3): 1881-1893, July-Sept. 2017. tab
Article in English | LILACS | ID: biblio-886722

ABSTRACT

ABSTRACT There is a huge lack of researches that evaluate the nutritional limits in tree species used in urban forestry, especially in terms of micronutrients. This study aimed to establish limits and range of micronutrients levels for the proper development of tree species utilized in urban forestry. The study was conducted in the city of Santa Maria-RS-Brazil. Through forest inventory, 23 forest species present in urban forest were selected, and 05 vegetative branches of each tree were collected, in which the contents of B, Cu, Fe, Mn and Zn were analyzed. Ranges of micronutrients' contents were developed for class limits criteria. Nutritional problems were detected for B, Cu and Zn in G. robusta and S. cumini, indicating a need of fertilization and management of these trees. The levels of Mn were within an adequate range only for the species C. illinoensis and H. chrysotrichus. The contents of B were higher than the level considered adequate for H. chrysotrichusand M. nigra. The rates of Fe showed high levels for E. japonica, H. chrysotrichusand S. babylonica. The estimated nutritional limits enable a greater control in the classification of the results for each tree species utilized in urban forestry.


Subject(s)
Forests , Micronutrients/analysis , Urban Population , Zinc/analysis , Cobalt/analysis , Copper/analysis , Iron/analysis , Manganese/analysis
9.
Braz. j. microbiol ; Braz. j. microbiol;48(3): 530-536, July-Sept. 2017. graf
Article in English | LILACS | ID: biblio-889139

ABSTRACT

Abstract Mangrove is an important ecosystem in the world. Mangrove ecosystems have a large capacity in retaining heavy metals, and now they are usually considered as sinks for heavy metals. However, the mechanism of why the soil of mangrove ecosystems can retain heavy metal is not certain. In this research, endophytic fungus Purpureocillium sp. A5 was isolated and identified from the roots of Kandelia candel. When this fungus was added, it protected the growth of K. candel under Cu stress. This can be illustrated by analyzing chlorophyll A and B, RWC and WSD to leaves of K. candel. Purpureocillium sp. A5 reduces uptake of Cu in K. candel and changes the pH characterization of soil. Furthermore, A5 increase the concentration of Cu complexes in soil, and it enhanced the concentration of carbonate-bound Cu, Mn-Fe complexes Cu and organic-bound Cu in soil. Nevertheless, a significant reduction of the Cu ion was noted among A5-treated plants. This study is significant and illustrates a promising potential use for environmental remediation of endophytes, and also may partially explain the large capacity of mangrove ecosystems in retaining heavy metals.


Subject(s)
Copper/metabolism , Rhizophoraceae/metabolism , Rhizophoraceae/microbiology , Endophytes/metabolism , Hypocreales/metabolism , Soil/chemistry , Soil Microbiology , Plant Roots/metabolism , Plant Roots/microbiology , Copper/analysis , Endophytes/isolation & purification , Endophytes/genetics , Hypocreales/isolation & purification , Hypocreales/genetics
10.
Braz. j. biol ; Braz. j. biol;76(2): 396-401, Apr.-June 2016. tab
Article in English | LILACS | ID: lil-781383

ABSTRACT

Abstract The effects of seasonal variations and the methods of collection of propolis produced by Africanized honey bees Apis mellifera Linnaeus, 1758, on the composition of constituent minerals such as magnesium (Mg), zinc (Zn), iron (Fe), sodium (Na), calcium (Ca), copper (Cu), and potassium (K) were evaluated. Propolis was harvested from 25 beehives by scraping or by means of propolis collectors (screen, “intelligent” collector propolis [ICP], lateral opening of the super [LOS], and underlay method). During the one-year study, the propolis produced was harvested each month, ground, homogenized, and stored in a freezer at -10 ºC. Seasonal analyses of the mineral composition were carried out by atomic absorption spectrophotometry and the results were evaluated by analysis of variance (ANOVA), followed by Tukey-Kramer’s test to compare the mean values (p<0.05). The results showed that seasonal variations influence the contents of 5 minerals (Mg, Fe, Na, Ca, and Cu), and the propolis harvesting method affects the contents of 4 minerals (Mg, Zn, Fe, and Ca).


Resumo A influência da sazonalidade e de métodos de produção de própolis por abelhas africanizadas Apis mellifera Linnaeus, 1758, sobre a concentração de magnésio (Mg), zinco (Zn), ferro (Fe), sódio (Na), cálcio (Ca), cobre (Cu) e potássio (K) foram avaliados. 25 colmeias foram utilizadas, e a colheita de propolis ocorreu por raspagem ou a partir de coletores (tela, coletor de própolis “inteligente” – CPI, abertura lateral da melgueira – ALM e calço). Durante um ano a própolis foi colhida mensalmente, homogeneizada e armazenada em freezer a -10 ºC. A análise sazonal de minerais foi realizada por espectrofotometria de absorção atômica e os resultados avaliados por análise de variância (ANOVA) seguida do teste de Tukey-Kramer para comparação de médias (p<0,05). Os resultados demostraram que a sazonalidade afetou o conteúdo de cinco minerais (Mg, Fe, Na, Ca e Cu) e os métodos de coleta afetaram o conteúdo de quatro minerais (Mg, Zn, Fe e Ca).


Subject(s)
Animals , Propolis/chemistry , Seasons , Bees , Minerals/analysis , Potassium/analysis , Sodium/analysis , Spectrophotometry, Atomic , Zinc/analysis , Calcium/analysis , Copper/analysis , Iron/analysis , Magnesium/analysis
11.
Rev. Assoc. Med. Bras. (1992, Impr.) ; Rev. Assoc. Med. Bras. (1992, Impr.);61(5): 407-410, Sept.-Oct. 2015. graf
Article in English | LILACS | ID: lil-766263

ABSTRACT

Summary Menkes disease is a congenital disorder caused by changes in copper metabolism derived from mutations in the ATP7A gene. It is characterized by physical and neurological alterations. In the neonatal period, these alterations can be nonspecific, which makes early diagnosis a challenge. Diagnosis can be suspected when there are low levels of ceruloplasmin and serum copper. Molecular analysis confirms the diagnosis. Treatment is parenteral administration of copper histidine. We report a familial case with molecular confirmation. The proband had clinical and biochemical suspicious. Treatment with copper histidine was indicated, but initiated at the age of 2 months and 27 days only. He did not present improvements and died at 6 months. The mother became pregnant again, a male fetus was identified and copper histidine was manufactured during pregnancy. He was born healthy, biochemical markers were reduced and treatment was indicated. Molecular analysis was performed confirming mutation in both the mother and the proband, while the other son did not have mutation, so treatment was discontinued. We support the clinical relevance of molecular confirmation for the correct diagnosis and genetic counseling, once clinical findings in the neonatal period are nonspecific and early treatment with parenteral copper histidine must be indicated.


Resumo A doença de Menkes é causada por uma alteração genética no metabolismo do cobre, por mutações no gene ATP7A. Caracteriza-se por alterações neurológicas e no exame físico. No período neonatal, essas alterações podem ser inespecíficas, o que torna o diagnóstico precoce um desafio. O diagnóstico pode ser suspeitado quando há baixos níveis séricos de cobre e ceruloplasmina. A análise molecular confirma o diagnóstico, e o tratamento deve ser feito com histidina de cobre. Nós relatamos um caso familial de doença de Menkes. O probando apresentava quadro clínico e alterações bioquímicas compatíveis com a doença de Menkes, em consulta com 1 mês de vida. O tratamento foi indicado, mas apenas iniciado com 2 meses e 27 dias. Ele não apresentou melhora clínica e veio a óbito com 6 meses. A mãe teve uma nova gestação, foi identificado um feto do sexo masculino e foi solicitada a manipulação da histidina de cobre ainda durante a gestação. O bebê nasceu saudável, os marcadores bioquímicos estavam diminuídos e o tratamento com histidina de cobre foi indicado. Realizamos a análise molecular, que confirmou mutação no gene ATP7A na mãe e no probando; porém, o outro filho não apresentava mutação e o tratamento foi interrompido. Nós defendemos a importância clínica da confirmação molecular para o correto diagnóstico e o aconselhamento genético da doença de Menkes, uma vez que os achados clínicos e as alterações bioquímicas no período neonatal são inespecíficos, e o tratamento com histidina de cobre parenteral deve ser rapidamente instituído.


Subject(s)
Female , Humans , Infant, Newborn , Male , Pregnancy , Histidine/analogs & derivatives , Menkes Kinky Hair Syndrome/genetics , Molecular Diagnostic Techniques/methods , Organometallic Compounds/therapeutic use , Adenosine Triphosphatases/genetics , Cation Transport Proteins/genetics , Ceruloplasmin/analysis , Copper/analysis , Fatal Outcome , Hair Diseases/diagnosis , Histidine/therapeutic use , Menkes Kinky Hair Syndrome/diagnosis , Menkes Kinky Hair Syndrome/drug therapy
12.
Article in English | IMSEAR | ID: sea-157696

ABSTRACT

The study aims to understand the relationship between increased serum levels of acute- phase reactants like Sialic Acid (SA) and transitional metals like Copper (Cu) which are indicated to be associated with the etiology and pathogenesis of type 2 diabetes mellitus (DM) and its various chronic complications. Serum SA and Cu levels, measured colorimetrically using Ehrilch’s reagent and Atomic Absorption Spectrophotometer (AAS) respectively among 30 cases each of type 2 DM with and without long-term complications, were significantly higher in all the cases compared to controls. The values are even higher in cases with complications, compared to diabetics without complications. Serum lipid profile was significantly altered in both the experimental groups. Serum SA and Cu levels are positively correlated to duration and degree of impaired glycemic status and altered lipid profile in type 2 DM. Measurement of these new serum markers can explain the inflammatory process, implicated towards the development of diabetic complications like cardiovascular diseases and microangiopathies thereby helping in early medical intervention.


Subject(s)
Adult , Aged , Biomarkers , Copper/analysis , Copper/blood , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/epidemiology , Diabetes Mellitus, Type 2/physiology , Female , Humans , Inflammation , Male , Middle Aged , N-Acetylneuraminic Acid/analogs & derivatives , N-Acetylneuraminic Acid/analysis , N-Acetylneuraminic Acid/blood
13.
Biol. Res ; 47: 1-8, 2014. graf, tab
Article in English | LILACS | ID: biblio-950767

ABSTRACT

BACKGROUND: Rootstocks play an essential role to determining orchard performance of fruit trees. Pyrus communisand Cydonia oblonga are widely used rootstocks for European pear cultivars. The lack of rootstocks adapted to different soil conditions and different grafted cultivars is widely acknowledged in pear culture. Cydonia rootstocks (clonal) and Pyrus rootstocks (seedling or clonal) have their advantages and disadvantages. In each case, site-specific environmental characteristics, specific cultivar response and production objectives must be considered before choosing the best rootstock. In this study, the influence of three Quince (BA 29, Quince A = MA, Quince C = MC) and a local European pear seedling rootstocks on the scion yield, some fruit quality characteristics and leaf macro (N, P, K, Ca and Mg) and micro element (Fe, Zn, Cu, Mn and B) content of 'Santa Maria' pear (Pyrus communis L.) were investigated. RESULTS: Trees on seedling rootstock had the highest annual yield, highest cumulative yield (kg tree(-1)), largest trunk cross-sectional area (TCSA), lowest yield efficiency and lowest cumulative yield (ton ha(-1)) in the 10th year after planting. The rootstocks had no significant effect on average fruit weight and fruit volume. Significantly higher fruit firmness was obtained on BA 29 and Quince A. The effect of rootstocks on the mineral element accumulation (N, K, Ca, Mg, Fe, Zn, Cu, Mn and B) was significant. Leaf analysis showed that rootstocks used had different mineral uptake efficiencies throughout the early season. CONCLUSION: The results showed that the rootstocks strongly affected fruit yield, fruit quality and leaf mineral element uptake of 'Santa Maria' pear cultivar. Pear seedling and BA 29 rootstock found to be more prominent in terms of several characteristics for 'Santa Maria' pear cultivar that is grown in highly calcareous soil in semi-arid climate conditions. We determined the highest N, P (although insignificant), K, Ca, Mg, Fe and Cu mineral element concentrations on the pear seedling and BA 29 rootstocks. According to the results, we recommend the seedling rootstock for normal density plantings (400 trees ha(-1)) and BA 29 rootstock for high-density plantings (800 trees ha(-1)) for 'Santa Maria' pear cultivar in semi-arid conditions.


Subject(s)
Plant Roots/metabolism , Plant Leaves/chemistry , Pyrus/classification , Pyrus/growth & development , Pyrus/chemistry , Desert Climate , Crop Production/methods , Fruit/metabolism , Phosphorus/analysis , Potassium/analysis , Rain , Soil , Temperature , Turkey , Zinc/analysis , Boron/analysis , Calcium/analysis , Copper/analysis , Rosaceae/physiology , Seedlings/growth & development , Seedlings/physiology , Iron/analysis , Magnesium/analysis , Manganese/analysis , Nitrogen/analysis
14.
Pesqui. vet. bras ; Pesqui. vet. bras;33(8): 1045-1048, ago. 2013. ilus
Article in Portuguese | LILACS | ID: lil-686084

ABSTRACT

O presente trabalho objetivou determinar a atividade sérica dos microminerais ferro, cobre, zinco e manganês em 30 equinos atletas da raça Puro-sangue Lusitano (PSL), antes e depois de exercícios, atestados por avaliações clínicas e laboratoriais. Amostras de sangue foram colhidas, antes e imediatamente após 20 minutos de exercício físico de trote e galope suaves realizados em pista de areia, para a realização das dosagens dos microminerais. A concentração dos microelementos foi determinada por espectrofotometria de absorção atômica. Observou-se que após o exercício, a atividade sérica de ferro não sofreu variações (P=0,2365), enquanto os valores de cobre se elevaram significativamente após o treinamento (P<0,001). Já o zinco e o manganês diminuíram após o exercício (P<0,001). Pode-se concluir que o exercício físico de curta duração pode gerar sudorese capaz de alterar as concentrações séricas de ferro, cobre, zinco e manganês em cavalos atletas da raça Puro-sangue Lusitano.


In order to evaluate the sera concentrations of iron, copper, zinc and manganese trace minerals in thirty Pure-bred Lusitano athlete horses before and after exercise certified by clinical and laboratory assessments were studied. Blood samples were taken before and after 20 minutes of a dairy trot and gallop slight training, perfomed in a sandy floor ring, to measure the mineral concentrations, by atomic absorption spectrophotometry. It was observed that after exercising, the serum activity of iron did not change (P=0.2365), whereas copper values have significantly risen after training (P<0.001). On the other hand, zinc and manganese trace minerals decreased after exercise (P<0.001). In conclusion, it is possible to consider that the short time exercise may trigger sweating and consequently to change the serum concentration of iron, cooper, zinc and manganese in Pure-bred Lusitano athlete horses.


Subject(s)
Animals , Copper/analysis , Iron/analysis , Manganese/analysis , Minerals/analysis , Zinc/analysis , Horses/metabolism , Equidae/classification , Spectrophotometry
15.
International Journal of Environmental Research. 2013; 7 (4): 989-994
in English | IMEMR | ID: emr-138334

ABSTRACT

The main sources of human exposure to trace metals for non-occupationally exposed individuals are inhalation and ingestion. Because the absorption rates of the metals by inhalation are significantly higher [up to 50-60%] than those by ingestion [between 3% and 10%], determination of trace metals in ambient air samples is of special interest. The main purpose of this study was to determine Fe, Zn and Cu in ambient air by combining pre-concentration methods and flame atomic absorption spectrophotometer [FAAS]. In the pre-concentration step, adsorption reagents including 4-[2-pyridyl-azo] Resorcinol [PAR]-loaded XAD-7, thioureasulphonamide polymeric resin and cupferron-activated carbon were used sequentially. Under the optimum conditions, seasonal distributions of Fe, Zn and Cu concentrations in ambient air of Elazig City, Turkey, were determined. Concentrations of Fe, Zn and Cu were found to be in the ranges of 154-416, 101-323 and 12-75 ng/m[3], respectively. High Zn levels determined in March and April can be attributed to the burning of coal at the beginning of spring


Subject(s)
Iron/analysis , Zinc/analysis , Copper/analysis , Particulate Matter , Spectrophotometry
16.
Braz. j. microbiol ; Braz. j. microbiol;44(1): 153-160, 2013. tab
Article in English | LILACS | ID: lil-676901

ABSTRACT

To study the deficiency of minerals and its relationship with Paratuberculosis, blood, serum, and fecal samples were obtained from 75 adult bovines without clinical symptoms of the disease and from two bovines with clinical symptoms of the disease, from two beef herds with a previous history of Paratuberculosis in the Province of Buenos Aires, Argentina. Serum samples were processed by ELISA and feces were cultured in Herrolds medium. Copper, zinc and iron in serum were quantified by spectrophotometry and selenium was measured by the activity of glutathione peroxidase. We also determined copper, zinc, iron and molybdenum concentrations in pastures and the concentration of sulfate in water. Mycobacterium avium subsp paratuberculosis (Map) was isolated from 17.3% of fecal samples of asymptomatic animals and from the fecal samples from the two animals with clinical symptoms. All the Map-positive animals were also ELISA-positive or suspect, and among them, 84.6% presented low or marginal values of selenium and 69.2% presented low or marginal values of copper. The two animals with clinical symptoms, and isolation of Map from feces and organs were selenium-deficient and had the lowest activity of glutathione peroxidase of all the animals from both herds. All the animals negative to Map in feces and negative to ELISA had normal values of Se, while 13.8% of animals with positive ELISA or suspect and culture negative presented low levels of Se. Half of the animals that were negative both for ELISA and culture in feces were deficient in copper but none of them presented low values of selenium. The content of molybdenum and iron in pasture was high, 2.5 ppm and 1.13 ppm in one herd and 2.5 ppm and 2.02 ppm in the other, respectively, whereas the copper:molybdenum ratio was 1.5 and 5.2, respectively. These results do not confirm an interaction between imbalances of the micronutrients and clinical Paratuberculosis, but show evidence of the relationship between selenium...


Subject(s)
Cattle , Copper/analysis , Glutathione Peroxidase/analysis , Glutathione Peroxidase/isolation & purification , Mycobacterium Infections , Mycobacterium avium/enzymology , Mycobacterium avium/isolation & purification , Paratuberculosis , Selenium/analysis , Zinc/analysis , Enzyme Activation , Enzyme-Linked Immunosorbent Assay , Methods , Minerals/analysis , Minerals/isolation & purification , Spectrophotometry
17.
Invest. clín ; Invest. clín;53(4): 342-352, dic. 2012. ilus, tab
Article in Spanish | LILACS | ID: lil-687427

ABSTRACT

En este trabajo se evaluó un nuevo microinyector de inyección en flujo para la determinación de Cu, Mg y Zn en células mononucleares de la sangre. Este dispositivo permitió analizar muestras en el orden de microlitros mediante espectrometría de absorción atómica con llama, es fácil de construir y adaptar al inyector convencional del espectrofotómetro de absorción atómica. En la determinación de Cu, Mg y Zn se obtuvieron límites de detección de 106, 65 y 37 µg L-1,respectivamente. En las pruebas de recuperación de estos elementos en leucocitos mononucleares se encontraron porcentajes de recuperación entre 98 y 110%.


In this paper we evaluated a new micro-flow injector for the determination of the concentrations of Cu, Mg and Zn in mononuclear blood cells. This device analyzed sample volumes in the order of microliters by flame atomic absorption spectrometry; it is inexpensive, and easy to build and to adapt to the conventional injector of the atomic absorption spectrophotometer. Detection limits of 106, 65 and 37 µg L-1 for Cu, Mg and Zn were obtained, respectively. The percentages of recovery tests were found between 98 and 110%.


Subject(s)
Humans , Copper/analysis , Leukocytes, Mononuclear/chemistry , Magnesium/analysis , Spectrophotometry, Atomic , Zinc/analysis
18.
Rev. Inst. Nac. Hig ; 43(2): 39-50, dic. 2012. tab, graf
Article in Spanish | LILACS, LIVECS | ID: lil-702800

ABSTRACT

El presente estudio tiene por objetivo comparar los niveles séricos de cobre (Cu), cinc (Zn) y el cociente molar Cu/Zn en 129 pacientes con artritis reumatoide (RA), en 94 individuos sanos como grupo control (GC) y 21 pacientes con enfermedades reumáticas no artríticas (NER), como la fibromialgia, espondilitis anquilosante entre otras. Las concentraciones séricas de Cu y Zn fueron analizadas por espectroscopía de absorción atómica en llama acoplada a un sistema de inyección en flujo continuo (EAA/FIA). Los resultados muestran un incremento significativo (p<0,05), del cobre sérico en los pacientes con AR en comparación con los otros grupos (NER y GC), este aumento fue directamente proporcional a la actividad inflamatoria de la enfermedad. El nivel medio de cinc sérico mostró una disminución progresiva. Asimismo, el detrimento sérico del cinc está relacionado con la evolución de la enfermedad o la pérdida de capacidad funcional del paciente con los niveles alterados de cobre observados en pacientes con AR activo y en etapa temprana de la enfermedad (p<0,05). Los cocientes molares de Cu/Zn fueron de 1,88; 1,14 y 1,18 para la AR, GC, y NER, respectivamente, demostrando que su modificación es más notoria que evaluar cada oligoelemento por separado. Estos hallazgos sugieren que existe una redistribución tanto del cobre como del cinc en muchos compartimientos del cuerpo, como respuesta inflamatoria. Por lo que niveles séricos de Cu, Zn y Cu/Zn molar puede ser un criterio valioso para la evaluación clínica y posiblemente, para el estudio y seguimiento de los pacientes con AR.


The aim of this study was to measure the serum levels of copper (Cu) and zinc (Zn) in 129 patients with rheumatoid arthritis (RA), in 94 healthy subjects (HS) and in 21 patients with non-rheumatic diseases (NRD) such as osteoarthritis, fibromyalgia, and ankylosing spondylitis. Serum concentrations of Cu and Zn were measured by flow injection analysis/flame atomic absorption spectroscopy (FIA/FAAS). The Cu/Zn molar ratio in serum also was evaluated in these patients. The result shown that the average concentration of serum copper in patients with RA was significantly increased (p <0.05), on comparing with other patients. This increase was directly proportional to staging or disease progression while zinc concentration was significantly lower in patients with RA, but no differences were found between the stages of the disease. The values of Cu/Zn ratio were 1.88, 1.14 and 1.18, for AR, HS, and NRD patients, respectively. In conclusion, in patients with RA serum copper tend to increase and serum Zn tend to decrease and Cu/Zn molar ratio increases significantly (p<0.05). In conclusion, our results suggest that serum Cu, Zn and Cu/Zn molar ratio could be valuable criteria for clinical evaluation and possibly follow-up study of patients with RA.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Aged , Arthritis, Rheumatoid/complications , Spectrum Analysis/instrumentation , Zinc/analysis , Copper/analysis , Spectrophotometry, Atomic/methods , Chemistry Techniques, Analytical , Public Health
19.
Arq. bras. med. vet. zootec ; Arq. bras. med. vet. zootec. (Online);64(5): 1232-1238, out. 2012. ilus, tab
Article in Portuguese | LILACS | ID: lil-655897

ABSTRACT

Os efeitos do uso de minerais complexados durante o pré-parto sobre a ocorrência de retenção de placenta foram avaliados em 135 vacas Holandesas de dois ou mais partos: grupo mineral iônico (69 animais) e grupo mineral complexado (66 animais). Em 55 desses animais foram também avaliadas as concentrações séricas da imunoglobulina G (IgG), Zn, Cu e a qualidade do colostro. O experimento foi realizado em delineamento inteiramente ao acaso, em arranjo em parcelas subdivididas. As concentrações séricas de IgG e dos microminerais foram avaliadas por análise de variância, sendo utilizados, respectivamente, os testes de Duncan e Fisher. A taxa de erro α admitida foi de 7%. Não foram observadas diferenças entre os grupos para ocorrência de retenção de placenta, qualidade do colostro, concentrações séricas de Zn e IgG (P>0,07), sendo observada diferença para a concentração de Cu (P<0,07). As concentrações de IgG foram diferentes nas semanas pré-parto avaliadas (P<0,07).


The effects of the use of complex minerals on the occurrence of retained placenta during pre-partum were valued on 135 Holstein cows from two or more deliveries. The animals were divided in two groups: ionic mineral (69 animals) and complexed mineral (66 animals). In 55 of these animals serum concentrations of imunoglobulin G (IgG), Zn and Cu and colostrum quality were also evaluated. The experiment was conducted in complete randomized split-plot design, serum IgG and trace minerals were evaluated by analysis of variance and used, respectively, Duncan's test and Fisher. The α error rate of 7% was accepted. There were no differences between groups for the occurrence of retained placenta, colostrum quality and serum concentrations of Zn and IgG (P>0.07), a difference was observed for Cu (P<0.07) concentrations. The IgG concentrations were different on the weeks pre partum evaluated (P <0.07).


Subject(s)
Animals , Cattle , Cattle/metabolism , /analysis , Minerals , Placenta, Retained/veterinary , Copper/analysis , Zinc/analysis
20.
Rev. biol. trop ; Rev. biol. trop;60(3): 1019-1116, Sept. 2012. graf, tab
Article in Spanish | LILACS | ID: lil-659573

ABSTRACT

Nutrients cycling is a fundamental component in the functioning of forest ecosystems. Leaves of different forest species observe specific chemical composition, and some seasonal differences in biomass production, may be related to climate fluctuations and/or changes in plant phenology and the variation on nutrient contents. The objective of this study was to analyze the seasonal variability of Fe, Mn, Cu and Zn concentrations in leaves of Gleditsia amorphoides (Ga); Patagonula americana (Pa); Maclura tinctoria (Mt) and Astronium balansae (Ab) from Colonia Benítez Strict Nature Reserve (Chaco, Argentina). The leaves of each forest species were collected monthly, dried at 70ºC and their weight by tree species were recorded. Samples for analytical determinations were prepared by humid oxidation of organic substances from vegetal nets, using oxidant acids such as ternary mixture of HNO3-H2SO4-HClO4. After digestion, total micronutrients leaves concentrations were determined by atomic absortion spectrophotometry. Leaves Fe highest values were detected during fall in Mt (76.1mg/kg), Pa (75.2mg/kg), Ab (59.5mg/kg) and Ga (45.3mg/kg). Highest foliar concentrations of Mn were detected in Pa (54.0mg/kg), Ga (50.0mg/kg), Mt (48.0mg/kg), and Ab with the lower Mn concentration (39.7mg/ kg). No significant differences were found for Cu between the different forest species, standing for Pa (11.3mg/ kg), Ga and Mt (11.0mg/kg) and Ab (10.4mg/kg). With regard to leaves Zn, highest concentrations were found in Mt (347.4mg/kg), Ga (319.9mg/kg), Pa (280.2mg/kg) and Ab (255.7mg/kg). Generally, a marked seasonal variation was observed for Mn and Cu concentrations (except in Ga) and Zn (except in Pa), with no statistical differences for Fe. in the species studied, the concentration of micronutrients analyzed showed a marked and erratic seasonal variation, attributed to the relationship established between the native species, and the strong influence of the environment over a long period of time, especially temperature and rainfall.


El ciclo de nutrientes es uno de los componentes fundamentales en el funcionamiento de los ecosistemas forestales. Las hojas de diferentes especies forestales muestran distinta composición química y diferencias estacionales en la producción de biomasa atribuidas a las fluctuaciones climáticas y/o cambios en la fenología de las plantas y a la variación en el contenido de nutrientes. El objetivo del trabajo fue analizar la variación estacional de las concentraciones de Fe, Mn, Cu y Zn en hojas de: Gleditsia amorphoides (Ga), Patagonula americana (Pa), Maclura tinctoria (Mt) and Astronium balansae (Ab). El sitio de estudio fue localizado en la Reserva Natural Estricta de Colonia Benítez (Chaco, Argentina). Las hojas de cada especie forestal fueron recogidas mensualmente, secadas a 70ºC, se determinó su peso seco y posteriormente se molió. El análisis foliar se realizó por oxidación húmeda empleando una mezcla ternaria HNO -H SO -HclO. Después de la digestión la concentración total de micronutrientes foliares fue determinada por espectrometría de absorción atómica. Las mayores concentraciones de Fe fueron para Mt (76.1mg/kg), Pa (75.2mg/kg), Ab (59.5mg/ kg) y Ga (45.3mg/kg). En Mn las más altas concentraciones se detectaron en Pa (54.0mg/kg), Ga (50.0mg/kg), Mt (48.0mg/kg), y la menor en Ab (39.7mg/kg). No se hallaron diferencias significativas entre las especies forestales para Cu foliar en Pa (11.3mg/kg), Ga y Mt (11.0mg/kg) y Ab (10.4mg/kg). Las mayores concentraciones foliares de Zn fueron en Mt (347.4mg/kg), Ga (319.9mg/kg), Pa (280.2mg/kg) y Ab (255.7mg/kg). En las especies forestales estudiadas, la concentración de los micronutrientes analizados mostraron una errática variación estacional, que puede ser atribuida a las relaciones establecidas entre las especies nativas bajo la fuerte influencia del ambiente, especialmente temperaturas y precipitaciones, durante un largo período de tiempo.


Subject(s)
Metals, Heavy/analysis , Micronutrients/analysis , Plant Leaves/chemistry , Trees/chemistry , Argentina , Copper/analysis , Iron/analysis , Manganese/analysis , Seasons , Spectrophotometry, Atomic , Zinc/analysis
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