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1.
Rev. Assoc. Med. Bras. (1992) ; 68(1): 87-93, Jan. 2022. tab, graf
Article in English | LILACS | ID: biblio-1360707

ABSTRACT

SUMMARY OBJECTIVE: The aim of this study was to evaluate the hepatoprotective effect and mechanism of action of artichoke leaf extract in hepatic ischemia/reperfusion injury. METHODS: Rats were divided into three groups such as sham, control, and artichoke leaf extract groups. Antioxidant enzyme activities and biochemical parameters were examined from the tissue and serum obtained from the subjects. Histopathological findings were scored semiquantitatively. RESULTS: Statistically, the antioxidant activity was highest in the artichoke leaf extract group, the difference in biochemical parameters and C-reactive protein was significant compared with the control group, and the histopathological positive effects were found to be significantly higher. CONCLUSIONS: As a result, artichoke leaf extract had a hepatoprotective effect and that this effect was related to the antioxidant and anti-inflammatory effects of artichoke.


Subject(s)
Animals , Rats , Reperfusion Injury/prevention & control , Reperfusion Injury/drug therapy , Cynara scolymus , Plant Extracts/therapeutic use , Plant Extracts/pharmacology , Liver , Antioxidants/metabolism , Antioxidants/therapeutic use , Antioxidants/pharmacology
2.
Article in Chinese | WPRIM | ID: wpr-828422

ABSTRACT

In this article, the foreign and domestic literature on alien plant medicine Cynara scolymus was reviewed to explore its properties and functions in traditional Chinese medicine theory, and provide theoretical basis for clinical application and reasonable compatibility. Based on the literature databases of PubMed, Web of Science, Embase, the Cochrane Library, CNKI, VIP and Sinomed, the articles with high reliability related to C. scolymus were screened out and the obtained articles were systematically classified according to clinical application, chemical compositions, pharmacological action, toxic and side effects, etc. In the analysis with traditional Chinese medicine theory, it is concluded that: C. scolymus tastes bitter and slightly cold, attributing to spleen, stomach, liver and gall meridians. It has the functions of eliminating accumulation and guiding stagnation, regulating Qi-flowing for harmonizing stomach, clearing away dampness and heat, resolving turbidity and lowering blood lipids. It can be used for the treatment of dyspepsia, diet reduction, vomiting, nausea, abdominal distention, hypochondriac pain, jaundice, hyperlipidemia, etc. Through the analysis and research of the relevant literature on C. scolymus, the properties and functions of the drug were clarified, which could provide a theoretical basis for further animal experiments and clinical research. The research model of "traditional Chinese medicine theory" for alien plant medicines can provide reference for the introduction and research of botanical drugs around the world, which can greatly enrich Chinese medicine resources and is of great significance for promoting the sustainable development of traditional Chinese medicine.


Subject(s)
Animals , Cynara scolymus , Drugs, Chinese Herbal , Medicine, Chinese Traditional , Meridians , Reproducibility of Results
3.
Journal of Integrative Medicine ; (12): 329-334, 2018.
Article in English | WPRIM | ID: wpr-691051

ABSTRACT

<p><b>BACKGROUND</b>Transcription factor 7-like 2 (TCF7L2)-rs7903146 polymorphism is associated with increased risk of type 2 diabetes. The response of insulin and insulin resistance to artichoke leaf extract (ALE) may be affected by TCF7L2-rs7903146 polymorphism.</p><p><b>OBJECTIVE</b>This study examined the effects of ALE supplementation on metabolic parameters of the TCF7L2-rs7903146 polymorphism in patients with metabolic syndrome (MetS).</p><p><b>DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS</b>This double-blind clinical trial was conducted on 80 patients with MetS in Sina Clinic, Khoy, Iran. The patients were randomized into ALE or placebo groups to receive either ALE (1800 mg/d as four tablets) or matching placebo for 12 weeks.</p><p><b>MAIN OUTCOME MEASURES</b>Anthropometric indices, blood pressure, glucose and lipid profile levels were measured before and after the study. Moreover, patients were genotyped for TCF7L2 polymorphism.</p><p><b>RESULTS</b>ALE supplementation decreased insulin level and the homeostasis model assessment of insulin resistance (HOMA-IR) in patients with the TT genotype of TCF7L2-rs7903146 polymorphism (P < 0.05). There was no significant interaction between blood pressure, glucose and lipid profile response to ALE supplementation.</p><p><b>CONCLUSION</b>The responses of insulin and HOMA-IR to ALE supplementation have shown an interaction with single-nucleotide polymorphism rs7903146 in TCF7L2.</p><p><b>TRIAL REGISTRATION</b>Iranian Registry of Clinical Trial IRCT201409033320N9.</p>


Subject(s)
Adult , Blood Glucose , Metabolism , Cynara scolymus , Dietary Supplements , Double-Blind Method , Female , Genotype , Humans , Insulin , Blood , Insulin Resistance , Genetics , Male , Metabolic Syndrome , Blood , Drug Therapy , Genetics , Middle Aged , Phytotherapy , Plant Extracts , Pharmacology , Polymorphism, Single Nucleotide , Transcription Factor 7-Like 2 Protein , Genetics
4.
São José dos Campos; s.n; 2016. 66 p. ilus, tab, graf.
Thesis in Portuguese | LILACS, BBO | ID: biblio-867657

ABSTRACT

A baixa citotoxicidade e ação anti-inflamatória são características interessantes para enxaguatórios bucais, dentifrícios e medicamentos de uso odontológico. Sendo assim, é necessário avaliar estas propriedades nos extratos glicólicos vegetais que podem ser ingredientes das formulações desses produtos. O presente estudo avaliou as seguintes atividades biológicas para cada um dos extratos glicólicos de Cynara cardunculus var. scolymus (L.) Fiori (alcachofra), Myracrodruon urundeuva Allemão (aroeira-do-sertão) e Camellia sinensis (L.) Kuntze(chá verde) em culturas de macrófagos de camundongo (RAW 264.7) pelo tempo de exposição de 5 min e 24 h: a) atividade citotóxica pelo método do MTT em 11 diluições seriadas, sendo que a concentração inicial dos extratos foi de 200mg/mL. b) atividade anti-inflamatória, após estímulo com lipopolissacarídeo (LPS) de Escherichia coli pelo método ELISA quantificou IL-1β, TNF-α e IL-10. Os resultados foram analisados estatisticamente por ANOVA e teste de Tukey, com p ≤ 5%. Acitotoxicidade dos extratos foi dose e tempo dependentes. No tempo de exposição de 5 min, o extrato de alcachofra apresentou citotoxicidade na concentração de 200 mg/mL. No tempo de exposição de 24 h, o chá verde apresentou citotoxicidade nas concentrações ≥ 50 mg/mL, aroeirado-sertão A baixa citotoxicidade e ação anti-inflamatória são características interessantes para enxaguatórios bucais, dentifrícios e medicamentos de uso odontológico. Sendo assim, é necessário avaliar estas propriedades nos extratos glicólicos vegetais que podem ser ingredientes das formulações desses produtos. O presente estudo avaliou as seguintes atividades biológicas para cada um dos extratos glicólicos de Cynara cardunculus var. scolymus (L.) Fiori (alcachofra), Myracrodruon urundeuva Allemão (aroeira-do-sertão) e Camellia sinensis (L.) Kuntze(chá verde) em culturas de macrófagos de camundongo (RAW 264.7) pelo tempo de exposição de 5 min e 24 h: a) atividade citotóxica pelo ...


he low cytotoxicity and anti-inflammatory action are interesting featuresfor mouthwashes, toothpastes and dental medication. Therefore, it isnecessary to evaluate these properties in plant glycolic extracts which canbe ingredients of the formulation of these products. This study evaluatedthe following biological activities for each of the glycolic extracts of Cynaracardunculus var. scolymus (L.) Fiori (artichoke), Myracrodruon urundeuvaAllemão (pepper tree) and Camellia sinensis (L.) Kuntze (green tea) inmouse macrophage cultures (RAW 264.7) by the exposure time of 5 minand 24 h. a) cytotoxicity by MTT method in 11 serial dilutions, the initialconcentration of the extracts was 200 mg/mL. b) Anti-inflammatory activityafter stimulation with lipopolysaccharide (LPS) of Escherichia coli wasperformed by immunoenzymatic test (ELISA) using specific antibodies toquantify IL-1β, TNF-α and IL-10. The results were subordinated tostatistical analyses (ANOVA and Turkey test), with p ≤ 0,05. Thecytotoxicity of the extracts was dependent on dose and time. At 5 minexposure time, artichoke extract showed cytotoxicity at a concentration of200 mg/mL. At 24 h exposure time, green tea showed cytotoxicity at theconcentrations ≥ 50 mg/mL, pepper tree stimulated the proliferation ofmacrophages in the concentrations of 0,39mg/mL to 12.5 mg/mL andartichoke showed cytotoxicity at the concentrations ≥ 12.5 mg/mL. Greentea extract showed anti- inflammatory potential at a concentration of 12.5mg/mL and was dependent on dose, promoting a decrease production ofpro-inflammatory cytokines as IL-1β and TNF-α. Pepper tree presentedimmunopotentiating action at a concentration of 12.5 mg/mL at 24 h ofexposure time. Regarding artichoke, there is a need for further studies toidentify their immunomodulatory action


Subject(s)
Cynara scolymus , Cytotoxicity, Immunologic
6.
São José dos Campos; s.n; 2016. 66 p. il., tab., graf..
Thesis in Portuguese | LILACS, BBO | ID: biblio-905458

ABSTRACT

A baixa citotoxicidade e ação anti-inflamatória são características interessantes para enxaguatórios bucais, dentifrícios e medicamentos de uso odontológico. Sendo assim, é necessário avaliar estas propriedades nos extratos glicólicos vegetais que podem ser ingredientes das formulações desses produtos. O presente estudo avaliou as seguintes atividades biológicas para cada um dos extratos glicólicos de Cynara cardunculus var. scolymus (L.) Fiori (alcachofra), Myracrodruon urundeuva Allemão (aroeira-do-sertão) e Camellia sinensis (L.) Kuntze (chá verde) em culturas de macrófagos de camundongo (RAW 264.7) pelo tempo de exposição de 5 min e 24 h: a) atividade citotóxica pelo método do MTT em 11 diluições seriadas, sendo que a concentração inicial dos extratos foi de 200mg/mL. b) atividade anti-inflamatória, após estímulo com lipopolissacarídeo (LPS) de Escherichia coli pelo método ELISA quantificou IL-1, TNF- e IL-10. Os resultados foram analisados estatisticamente por ANOVA e teste de Tukey, com p 5%. A citotoxicidade dos extratos foi dose e tempo dependentes. No tempo de exposição de 5 min, o extrato de alcachofra apresentou citotoxicidade na concentração de 200 mg/mL. No tempo de exposição de 24 h, o chá verde apresentou citotoxicidade nas concentrações 50 mg/mL, aroeirado- sertão estimulou a proliferação de macrófagos na concentração de 0,39 mg/mL a 12,5 mg/mL e a alcachofra apresentou citotoxicidade nas concentrações 12,5 mg/mL. O extrato de chá verde apresentou potencial anti-inflamatório na concentração de 12,5 mg/mL e foi dose dependente, promovendo diminuição da produção de citocinas próinflamatória, como IL-1ß e TNF-. A aroeira-do-sertão apresentou ação imunopotenciadora na concentração de 12,5 mg/mL no tempo de exposição de 24 h. Com relação a alcachofra há necessidade de novos estudos para identificar sua ação imunomoduladora(AU)


The low cytotoxicity and anti-inflammatory action are interesting features for mouthwashes, toothpastes and dental medication. Therefore, it is necessary to evaluate these properties in plant glycolic extracts which can be ingredients of the formulation of these products. This study evaluated the following biological activities for each of the glycolic extracts of Cynara cardunculus var. scolymus (L.) Fiori (artichoke), Myracrodruon urundeuva Allemão (pepper tree) and Camellia sinensis (L.) Kuntze (green tea) in mouse macrophage cultures (RAW 264.7) by the exposure time of 5 min and 24 h. a) cytotoxicity by MTT method in 11 serial dilutions, the initial concentration of the extracts was 200 mg/mL. b) Anti-inflammatory activity after stimulation with lipopolysaccharide (LPS) of Escherichia coli was performed by immunoenzymatic test (ELISA) using specific antibodies to quantify IL-1, TNF- and IL-10. The results were subordinated to statistical analyses (ANOVA and Turkey test), with p 0,05. The cytotoxicity of the extracts was dependent on dose and time. At 5 min exposure time, artichoke extract showed cytotoxicity at a concentration of 200 mg/mL. At 24 h exposure time, green tea showed cytotoxicity at the concentrations 50 mg/mL, pepper tree stimulated the proliferation of macrophages in the concentrations of 0,39mg/mL to 12.5 mg/mL and artichoke showed cytotoxicity at the concentrations 12.5 mg/mL. Green tea extract showed anti- inflammatory potential at a concentration of 12.5 mg/mL and was dependent on dose, promoting a decrease production of pro-inflammatory cytokines as IL-1 and TNF-. Pepper tree presented immunopotentiating action at a concentration of 12.5 mg/mL at 24 h of exposure time. Regarding artichoke, there is a need for further studies to identify their immunomodulatory action(AU)


Subject(s)
Humans , Cytotoxicity, Immunologic , Tea/adverse effects , Cynara scolymus/adverse effects , Bursera
7.
Dominguezia ; 31(1): 37-39, 2015. ilus
Article in Spanish | LILACS, MTYCI | ID: biblio-911266

ABSTRACT

La detección de insectos y el mal aspecto en las hierbas medicinales que se comercializan en la herboristería resulta una problemática cotidiana. Con el objetivo de contribuir con la mejora de la calidad final de estas hierbas, se colectaron a campo cuatro drogas vegetales de amplia utilización en la medicina tradicional. Se comparó su calidad con la de las provenientes de un acopiador y se realizó un minucioso examen para la detección e identificación de insectos, sus rastros o vestigios. Posteriormente, se ensayaron algunos métodos físicos para evitar la afectación de las hierbas por parte de esos insectos. Las características de calidad final de las hierbas provenientes de recolección propia resultaron superiores a las provenientes de un acopiador. Desde el punto de vista entomológico, se identificaron las principales especies contaminantes y se encontraron los mejores métodos y condiciones para evitar su proliferación.


Subject(s)
Cynara scolymus , Lappa arctium , Malva , Plants, Medicinal , Taraxacum , Argentina , Medicine, Traditional
8.
Article in Korean | WPRIM | ID: wpr-210254

ABSTRACT

The purpose of this study was to determine the optimal composite recipe of rice muffin using three different amounts of Chinese artichoke (Stachys sieboldii MIQ) powder, brown sugar, and egg. Response surface methodology (RSM) was used to obtain 16 experimental points (including three replicates of Chinese artichoke powder, brown sugar, and egg), and the Chinese artichoke rice muffin formulation was optimized using rheology. The results of the sensory evaluation showed very significant values for color, texture, sweetness, and overall quality (P<0.05). The results of the color, texture, and chemical analyses showed significant values for crumb redness (P<0.01), crumb yellowness (P<0.05), crust redness (P<0.05), crust yellowness (P<0.001), crust lightness (P<0.05), adhesiveness (P<0.01), springiness (P<0.001), gumminess (P<0.01), cohesiveness (P<0.05), moisture content (P<0.05), and sweetness (P<0.05). As a result, optimum formulations obtained by numerical and graphical methods were found to be 8.28 g of Chinese artichoke powder, 66.20 g of brown sugar, 111.72 g of sticky rice powder, 30 g of rice powder, and 59.37 g of egg.


Subject(s)
Adhesiveness , Asians , Cynara scolymus , Humans , Ovum , Rheology
9.
Rev. bras. plantas med ; 15(4): 494-502, 2013. graf, tab
Article in Portuguese | LILACS | ID: lil-695233

ABSTRACT

O uso de plantas diuréticas e laxativas é uma alternativa medicamentosa para muitas pessoas que objetivam o emagrecimento com menores efeitos adversos. A avaliação microbiológica é um requisito essencial para a garantia de qualidade dos produtos. A finalidade deste trabalho foi avaliar a qualidade microbiológica de cápsulas e chás de alcachofra (Cynara scolymus L.), centella asiática (Hydrocotile asiatica L.), fucus (Fucus vesiculosus L.), e sene (Cassia acutifolia Delile), através da contagem de micro-organismos viáveis totais e pesquisa de patógenos. Na contagem de micro-organismos viáveis, os chás analisados foram aprovados, pois apesar de apresentarem uma carga microbiana elevada, esta se encontrava dentro das especificações, entretanto, 16,66% e 66,66% das cápsulas analisadas foram reprovadas por apresentaram quantidades superiores de bactérias e fungos, respectivamente. Na pesquisa de patógenos, 76% das amostras (88% dos chás e 58% das cápsulas) apresentaram um ou mais de um tipo de micro-organismo. Salmonella sp. esteve presente em 33% das amostras evidenciando a qualidade microbiológica insatisfatória dos produtos encontrados no mercado. Estes resultados demonstram a necessidade da realização do controle de qualidade tanto das matérias-primas vegetais, quanto dos produtos acabados, através do controle e fiscalização rigorosa, com adoção de medidas regulamentadoras e educativas.


The use of laxative and diuretic herbal drugs is an alternative therapy for many people looking for loosing weight with fewer side effects. The microbiological evaluation is an essential requirement for the quality assurance of products. This study aimed to evaluate the microbial quality of artichoke (Cynara scolymus L.), centella (Hydrocotile asiatica L.), fucus (Fucus vesiculosus L.), and Senna (Cassia acutifolia Delile) capsules and teas, by counting the total viable aerobic microorganisms and through tests for specified microorganisms. On the total viable aerobic microorganisms count, the teas analyzed were approved, because although they had a high microbial load, this was in accordance with the specifications, however 16.66% and 66.66% of the capsules analyzed were rejected because they presented higher amounts of bacteria and fungi, respectively. In relation to the tests for specified microorganisms, 76% of the samples (88% of the teas and 58% of the capsules) presented one or more than one type of microorganisms. Salmonella sp. was present in 33% of the samples, showing the unsatisfactory microbial quality of the products in the market. These results demonstrate the necessity of performing quality control both on herbal raw material and finished products, through a rigorous control and inspection, adopting regulatory and educational measures.


Subject(s)
Capsules/classification , Microbiological Techniques/analysis , Teas, Medicinal/classification , Centella/adverse effects , Cynara scolymus/adverse effects , Plants, Medicinal/metabolism
10.
Diagn. tratamento ; 17(2)abr. 2012.
Article in Portuguese | LILACS | ID: lil-646024

ABSTRACT

Introdução: A alcachofra é uma planta utilizada na culinária e dita com diversas propriedades medicinais.Objetivo: Verificar evidências do uso da alcachofra no tratamento de doenças.Métodos: Busca sistematizada da literatura por meio eletrônico, incluindo estudos que avaliem o uso da alcachofra namedicina.Resultados: Uma revisão sistemática mostrou resultados favoráveis a alcachofra na redução do colesterol total[intervalo de confiança (IC) de 0,40 a 0,79] e do LDL-colesterol (IC de 0,34 a 0,78). Dois ensaios clínicos randomizadoschegaram à mesma conclusão. Um estudo clínico mostrou benefício do uso da alcachofra em pacientes com síndromedo intestino irritável e outro mostrou que ela pode ser útil nas dispepsias funcionais. Um ensaio clínico constatou oaumento da atividade antioxidante total em remadores submetidos ao estresse físico e suplementados com alcachofra.Em todos os estudos não há ocorrência de efeitos adversos.Conclusão: Os estudos encontrados mostram evidências de uma ação benéfica da alcachofra nas hiperliproteinemias e nadispepsia funcional. Como sua eficácia é dose-dependente, não se pode depender apenas da ingestão de alcachofra por meioda alimentação. Os dislipidêmicos e dispépticos funcionais terão que se utilizar dos extratos vendidos comercialmente.


Subject(s)
Humans , Male , Female , Adult , Antioxidants/therapeutic use , Cynara scolymus , Dyslipidemias/therapy , Dyspepsia/therapy , Irritable Bowel Syndrome/therapy
11.
Diagn. tratamento ; 17(2)abr. 2012.
Article in Portuguese | LILACS | ID: lil-646025

ABSTRACT

Dentre os pacientes que apresentam episódios de transtornodepressivo maior, os que são fumantes, os que sentem não tercontrole sobre as circunstâncias da vida e os que têm uma históriaanterior de depressão têm probabilidade maior de teremrecidiva em seis anos.


Subject(s)
Humans , Male , Female , Adult , Cynara scolymus , Plants, Medicinal
12.
Egyptian Journal of Hospital Medicine [The]. 2012; 47: 279-290
in English | IMEMR | ID: emr-170354

ABSTRACT

Excessive free radicals are caused by unnatural environmental influences such as air pollution, radiation, cigarette smoke, factories, pesticides, food contaminants and a myriad of other factor that are part of our modern life. Hypercholesterolaemia is directly associated with an increased risk of coronary heart disease [CHD]. Cynara scolymus L. [Artichoke] grows in Egypt and other countries. It is used as foods and has medicinal properties. Artichoke extracts have been shown to produce various pharmacological effects, such as the inhibition of cholesterol biosynthesis and low density lipoprotein [LDL] oxidation. The present study aims to evaluate the antioxidative activities and radioprotector role of cynara scolymus L. [artichoke] against hyperlipidemic induced by gamma- irradiation in male rats. Male Swiss albino rats were orally administrated by artichoke [head or leaves] [10% mg/rat/day] using suitable stomach tube [6weeks, 45 days] before exposure to a single dose [6.5 Gy] of whole body gamma radiation. Levels of lipids peroxides [MDA], reduced glutathione content [GSH], superoxide dismutase [SOD], total cholesterol [TC], triacylglycerol [TG], high density lipoprotein [HDL-C] and low density lipoprotein [LDL-C] were investigated in serum. The results revealed that gamma radiation led to significant increase in MDA, TC, TG and LDL, Meanwhile, significant decrease in GSH and SOD, but groups administrated with Artichoke [head and leaf] before whole body gamma irradiation, artichoke exerted noticeable amelioration against the radiation induced changes in most of the biochemical tested parameters


Subject(s)
Male , Animals, Laboratory , Gamma Rays/adverse effects , Cynara scolymus/drug effects , Rats , Antioxidants
13.
Braz. j. pharm. sci ; 45(2): 201-208, Apr.-June 2009. graf, tab
Article in English | LILACS | ID: lil-525916

ABSTRACT

The objective of this study was the investigation of a potential influence of artichoke leaf extract (ALE) on the cell physiology and gene expression of phase I/II enzymes of human liver cells HepG2 and investigation on potential cell protective effects against ethanol-induced cell toxicity against HepG2 cells. Cell biological assays under in vitro conditions using HepG2 liver cells and investigation of mitochondrial activity (MTT test), proliferation assay (BrdU incorporation ELISA), LDH as toxicity marker, gene expression analysis by RT-PCR and enzyme activity of glutationtransferase. Artichocke extract, containing 27 percent caffeoylquinic acids and 7 percent flavonoids induced mitochondrial activity, proliferation and total protein content under in vitro conditions in human liver cells HepG2. These effects could not be correlated to the well-known artichoke secondary compounds cynarin, caffeic acid, chlorogenic acid, luteolin and luteolin-7-O-glucoside. The flavones luteolin and luteolin-7-O-glucoside had inhibitory effects at 100 µg/mL level on HepG2 cells, with luteolin being a significant stronger inhibitor compared to the respective glucoside. Artichoke leaf extract had minor stimulating effect on gene expression of CYP1A2, while CYP3A4, GGT, GPX2, GSR and GST were slightly inhibited. GST inhibition under in vitro conditions was also shown by quantification of GST enzyme activity. Induction of gene expression of CYP1A2 was shown to be supraadditive after simultaneous application of ethanol plus artichoke extract. Artichoke leaf extract exhibited cell protective effects against ethanol-induced toxicity within cotreatment under in vitro conditions. Also H2O2 damage was significantly inhibited by simultaneous artichoke incubation. Pre- and posttreatments did not exert protective effects. DMSO-induced toxicity was significantly reduced by pre-, post- and cotreatment with artichoke extract and especially with luteolin-7-O-glucoside...


O objetivo deste estudo foi a investigação de uma potente influência do extrato das folhas da alcachofra (ALE) na fisiologia celular e na expressão gênica de enzimas de fase I/II de células hepáticas humanas HepG2 e investigação no potencial efeito protetor celular em células HepG2 contra toxicidade celular induzida por etanol. Ensaios biológicos de células em condições in vitro usando células de fígado HepG2 e investigação da atividade mitocondrial (teste MTT), ensaio de proliferação, LDH como marcador de toxicidade, análise de expressão gênica por RT-PCR e atividade da enzima glutationa transferase. O extrato da alcachofra, contendo 27 por cento de ácidos cafeoilquínico e 7 por cento de flavonóides, induzem a atividade mitocondrial, proliferação e o teor de proteína total em condições in vitro em células hepáticas humanas HepG2. Estes efeitos não podem ser correlacionados aos compostos secundários conhecidos da alcachofra, cinarina, ácido cafeico, ácido clorogênico, luteolina e luteolin-7-O-glicosídeo. As flavonas luteolina e luteolin-7-O-glicosídeo possuem efeitos inibitórios em nível de 100 µg/mL em células HepG2, com a luteonina sendo uma inibidora significativamente mais forte comparada com o respectivo glicosídeo. O extrato das folhas de alcachofra possui um efeito mínimo da estimulação na expressão gênica de CYP1A2, enquanto CYP3A4, GGT, GPX2, GSR e GST foram sutilmente inibidos. A inibição de GST em condições in vitro também foi mostrada pela quantificação da atividade da enzima GST. Indução da expressão gênica de CYP1A2 mostrou-se supraaditiva após aplicação simultânea do etanol mais o extrato de alcachofra. O extrato das folhas de alcachofra exibiu efeitos protetores celulares frente à toxicidade induzida por etanol em co-tratamento em condições in vitro. Além disso, danos por H2O2 foram significativamente inibidos pela incubação simultânea do extrato de alcachofra. Pré e pós-tratamento não exerceram efeitos protetores. Toxicidade...


Subject(s)
Humans , Biological Assay , Cynara scolymus/chemistry , Hepatocytes , Phytotherapy , Plant Preparations/analysis
14.
Arab Journal of Biotechnology. 2009; 12 (1): 27-39
in English | IMEMR | ID: emr-90924

ABSTRACT

The present investigation was designed to evaluate oocyte competence including quality, meiotic progression and expression of ERK2 gene and activities of liver enzymes [ALT, AST and gamma-GT] in female mice fed diets containing sunflower oil repeatedly used in frying processes [FO]. The protective role of artichoke and green pepper against such potential dangers were also studied. Results revealed that mice fed FO diet had a significant decrease in number of good collected oocytes compared with those fed normal oil [NO] diet. Mice fed FO diet and artichoke extract at two levels, 0.5% [FO-A1] and 1% [FO-A2] as drinking water or FO diet plus dried ground green pepper at two levels, 5% [FO-G1] and 10% [FO-G2] had a significant increase in number of good collected oocytes compared with those fed FO diet. Animals fed FO-A1 or FO-G1 diets had the highest percentage of good oocytes. A statistically significant increase in the percentages of mature oocytes occurred in mice fed FO-A1, FO-A2, and FO-G1 diets as compared to those fed FO diet. The animals fed FO-G2 diet had a non-significant increase of percentages of mature oocytes than the animals fed FO diet. The expression of ERK2 gene was high in ovary tissues collected from female mice fed NO, FO, FO-A1, or FO-G1 diets. However, the ERK2 gene expression was low in mice fed FO-A2 or FO-G2 diets. Biochemical assay showed that mice fed FO diet had significant decrease in AST and ALT activities. A significant increase in enzyme activity of gamma- GT compared with those fed NO diet was also observed. A statistically significant increase in activities of AST and ALT enzymes was observed in mice fed FO-A2, FO-G1 and FO-G2 diets. However, mice fed FO-A1, FO-A2, FO-G1 and FO-G2 diets had significant decrease in the activity of gamma-GT enzyme compared with those fed FO diet. The present study demonstrated evidence that prolonged intake of diets containing sunflower oil repeatedly used in frying processes could be considered a cause of induced abnormalities in reproductive materials and liver enzymes activities of female mice. However, artichoke and green pepper proved to be suitable protectors against hazards of such toxic materials


Subject(s)
Female , Animals, Laboratory , Protective Agents , Helianthus , Capsicum , Mice , Reproduction , Liver Function Tests , Oocytes , Polymerase Chain Reaction , Cynara scolymus
15.
Medical Journal of Cairo University [The]. 2006; 74 (Supp. 2): 91-96
in English | IMEMR | ID: emr-79457

ABSTRACT

Cynara scolynius L. [Artichoke] grows in Egypt and other countries. Its green leaves and head are edible parts and used as foods due to their high nutritive value and medical properties. To study the effect of Cynara scolymus L. [balady artichoke] and its extract on liver cirrhosis of male rats. Sixty-four male albino rats, Sprague Dawley strain, weighing [200 +/- 10g] were housed in well-aerated wire cages. Induction of cirrhosis was carried out on rats except negative control group by subcutaneous injection of carbon tetrachloride [CCI[4]] that is diluted by paraffin oil [1:1] in a dose of 2ml/kg of body weight of rat twice in the week during the experimental feeding period. The rats [64] were divided into 8 groups [8 rats each], the first 1[st] and 2[nd] groups as control positive and negative groups [each 8 rats], the second main group [48 rat] was divided into six subgroups as follow: the 1[st] and 2[nd] subgroup fed on basal diet supplemented with powdered artichoke leaves in a dose of 15% and 30% respectively; the 3[rd] and 4[th] subgroups fed on basal diet supplemented with powdered artichoke heads in a dose of 15% and 30% respectively; the 5[th] and 6[th] subgroups fed on basal diet supplemented with artichoke extract in a dose of 3% and 6% respectively. At the end of the experimental period [two months] rats were fasted over night before sacrificing, blood was collected, centrifuged; serum was stored at-20°C until analysis. Total protein, alanine aminotransferase [ALT], aspartate aminotransferase [AST], Creatinine, urea and uric acid were estimated in serum. The results revealed that induction of cirrhosis results in significant decrease in serum level of total protein and a significant increase in AST. ALT, urea, creatinine and uric acid. The inclusion of leaves, heads and extracts of artichoke in the diet of cirrhotic rats lead to significant decrease of the significant increased level of AST, ALT, urea. creatinine, sometimes it might reached the normal level but for uric acid it has no decreasing effect on it, it continue to rise [>3 times]. For protein it increased the already decreased level. Carbon tetrachloride has direct toxic effect, which is clear by the presence of severe biochemical abnormalities in liver and kidney function. The natural antioxidant from artichoke improves these abnormalities


Subject(s)
Male , Animals, Laboratory , Cynara scolymus , Plant Extracts/analysis , Plant Leaves , Transaminases , Urea , Uric Acid , Creatinine , Blood Proteins , Alanine Transaminase , Rats, Sprague-Dawley , Liver , Aspartate Aminotransferases , Histology , Antioxidants
16.
Journal of Medicinal Plants. 2005; 4 (13): 1-10
in Persian | IMEMR | ID: emr-72086

ABSTRACT

Articholce [Cynara Scolymus L.] is one of non- native plants being cultivated in Iran. This plant is mostly used in foods, so that it is probably harmless. In this Article we are going to coucentrate on different stages of its cultivation and the clinical uses of it in relation with some clinical studies


Subject(s)
Cynara scolymus/growth & development , Cardiovascular Diseases/prevention & control , Liver/drug effects
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