Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 20
Filter
1.
Rev. medica electron ; 41(1): 203-210, ene.-feb. 2019.
Article in Spanish | LILACS, CUMED | ID: biblio-991338

ABSTRACT

RESUMEN El cáncer cérvico uterino, causa alrededor de 250 000 muertes anuales en el mundo y alrededor de 400 en Cuba, a pesar del esfuerzo que realiza el MINSAP, a través del Programa de Pesquisaje. Con el mismo se puede obtener el diagnóstico de lesiones precursoras del cáncer de cuello uterino, este diagnóstico citológico se realiza en Cuba a través del método de Richard y Barron que demuestra que existe un progreso citológico aparente hasta llegar al cáncer, que comienza con neoplasia intraepitelial (NICI a NICIII y carcinoma in situ), hasta finalmente el cáncer invasor. Por otro lado existe el método de Bethesda que responde casi todas las interrogantes que la citología plantea para su enfrentamiento, evidentemente los mayores aportes y revisiones se enfocan al manejo de las citologías atípicas de significado incierto, ya que no sólo presentan un mayor número de posibles evaluaciones, sino que representan el mayor porcentaje de citologías alteradas y la inclusión del VPH en las lesiones de bajo grado. En Cuba todavía se clasifica por el método de Richard y no se utiliza el Bethesda. Por la alta incidencia de esta entidad el propósito de este trabajo es emitir consideraciones sobre la implementación del sistema de Bethesda en el diagnóstico citológico de lesiones precancerosas del cérvix.


ABSTRACT The cervical-uterine cancer causes almost 250 000 death a year around the world and around 400 in Cuba in spite of the efforts made by the Public Health Ministry through the Screening Program. With it, the diagnosis of lesions that are predecessors of the cervical cancer could be reached. This cytological diagnosis is carried out through the Richard and Barron method, showing that there is an apparent cytological progress leading to the cancer that begins with intraepithelial neoplasia (NICI and NICIII and carcinoma in-situ) and ends in the invasive cancer. From the other hand there is the Bethesda methods answering to all the questions cytology ask for confronting it. Obviously the biggest contributions and reviews are focused in the management of the atypical cytologies with uncertain significance since they not only have a higher number of possible evaluations, but also represent the highest percent of the altered cytologies and the inclusion of the HPV in low grade lesions. The classification in Cuba is still made by the Richard method and the Bethesda one is not used. Due to the high incidence of this entity, the aim of this article is exposing considerations on the implementation of the Bethesda system in the cytological diagnosis of cervix pre-cancerous lesions.


Subject(s)
Humans , Female , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/pathology , Uterine Cervical Neoplasms/virology , Cytological Techniques/standards , Papillomavirus Infections/diagnosis , Papillomavirus Infections/pathology , Papillomavirus Infections/virology , Biopsy, Fine-Needle/methods , National Health Programs , Preventive Health Services , Disease Prevention
2.
Arq. bras. oftalmol ; 70(2): 343-346, mar.-abr. 2007. ilus
Article in Portuguese | LILACS | ID: lil-453180

ABSTRACT

Relatamos três casos de infecção corneana por Acanthamoeba sp em que foi possível detectar cistos do microorganismo com a técnica de citologia de impressão. Três pacientes encaminhados ao Laboratório de Doenças Externas Oculares em 2004 com alterações superficiais da córnea foram submetidos ao exame de citologia de impressão para investigação da presença de cistos de Acanthamoeba sp. Duas amostras foram obtidas da córnea de cada paciente e coradas com PAS, hematoxilina e Papanicolaou. Investigação microbiológica de rotina e cultura também foram realizadas após raspado da córnea. O cultivo das amostras e a citologia de impressão foram positivas para Acanthamoeba sp em todos os pacientes, ao passo que os raspados corados com Giemsa foram positivos em dois casos. A citologia de impressão revelou cistos de Acanthamoeba sp entre feixe de células epiteliais corneanas e como células isoladas. Foram observados cistos no epitélio de um dos pacientes com a citologia de impressão após três meses de tratamento, enquanto o raspado foi negativo. No exame anatomopatológico observaram-se cistos no epitélio e estroma de uma córnea receptora de um dos pacientes após transplante. Neste estudo, a citologia de impressão detectou com sucesso cistos de Acanthamoeba sp em pacientes com acometimento epitelial. Por tratar-se de método não invasivo, a técnica pode ser usada para facilitar o diagnóstico mais precoce da infecção por Acanthamoeba, sendo útil também no acompanhamento do tratamento da doença.


To describe three cases of corneal infection due to Acanthamoeba sp in which was possible to detect Acanthamoeba sp cysts by the corneal impression cytology technique. Three patients referred to the External Eye Disease Laboratory in 2004 with superficial corneal alterations were submitted to corneal specimen collection by impression cytology filter paper to investigate the presence of Acanthamoeba sp cysts. Two impression cytology samples were obtained from each patient and were stained by PAS, hematoxylin and Papanicolaou. Routine microbiological investigation and culture were also performed using corneal scraping. Positive culture and impression cytology for Acanthamoeba sp was observed in all patients while smears with Giemsa stain were positive in two. Impression cytology Acanthamoeba sp cysts were observed among sheets of corneal epithelial cells and as isolated cells. Cysts were also found in the superficial epithelium in one of these patients after treatment while corneal scraping did not reveal any cyst. Histopathology revealed cysts in the epithelium and stroma in a transplanted cornea in one of these patients. The first description of impression cytology as a diagnostic method for Acanthamoeba keratitis occurred recently. In this study corneal impression cytology detected Acanthamoeba sp cysts successfully in these patients with only superficial involvement. Impression cytology as a non invasive technique can be used to facilitate early recognition of Acanthamoeba infection playing a useful role in the follow-up of the disease.


Subject(s)
Humans , Animals , Acanthamoeba Keratitis/diagnosis , Acanthamoeba Keratitis/etiology , Acanthamoeba Keratitis/microbiology , Acanthamoeba Keratitis/parasitology , Acanthamoeba/isolation & purification , Contact Lenses, Hydrophilic/adverse effects , Cytodiagnosis/standards , Cytological Techniques/standards , Epithelium, Corneal/microbiology , Epithelium, Corneal/pathology , Staining and Labeling
3.
Rev. chil. obstet. ginecol ; 70(3): 152-155, 2005. tab
Article in Spanish | LILACS | ID: lil-449829

ABSTRACT

Objetivo: Evaluar la calidad del laboratorio de citología cervical para LIE de bajo y alto grado del Hospital Naval de Talcahuano a través de la correlación citohistológica. Material y Método: Estudio retrospectivo, descriptivo y analítico. Se revisaron 422 historias clínicas de pacientes ingresadas a la Unidad de Patología Cervical (UPC) del Hospital Naval de Talcahuano, en el período 1994-2004. En 204 casos hubo citología positiva para LIE, 83,3 por ciento tenían una biopsia bajo visión colposcópica, y 75 por ciento tenían una biopsia por escisión con asa electroquirúrgica. Como clasificación diagnóstica se empleó el sistema Bethesda. Resultados: No hubo diferencia significativa entre la media de edad en las pacientes que presentaban LIE de bajo y alto grado, tanto para el grupo con biopsia bajo visión colposcópica como para el grupo con biopsia con asa electroquirúrgica. En el grupo con biopsia bajo visión colposcópica hubo coincidencia citohistológica en 67,1 por ciento para LIE bajo grado, y 67,1 por ciento para LIE alto grado. En el grupo con biopsia con asa electroquirúrgica hubo coincidencia citohistológica en 59,7 por ciento para LIE bajo grado, y 73,7 por ciento para LIE alto grado. Existe correlación entre citología para LIE e histopatología tanto de biopsia bajo visión colposcópica, como por escisión con asa electroquirúrgica (p<0,001). Discusión: El promedio de edad de los pacientes que portaban un LIE bajo grado en ambos grupos de estudio fue superior a las series nacionales e internacionales. En la UPC del Hospital Naval (T) el screening citológico es confiable para LIE de bajo y alto grado.


Subject(s)
Adolescent , Adult , Humans , Female , Middle Aged , Carcinoma, Squamous Cell , Cytodiagnosis/methods , Mass Screening , Cervical Intraepithelial Neoplasia/epidemiology , Cervical Intraepithelial Neoplasia/pathology , Uterine Cervical Neoplasms/epidemiology , Uterine Cervical Neoplasms/pathology , Biopsy , Chile , Colposcopy , Electrosurgery , Epidemiologic Studies , Laboratories/standards , Quality Control , Retrospective Studies , Cytological Techniques/standards
4.
Acta cir. bras ; 20(supl.1): 78-81, 2005. ilus
Article in English | LILACS | ID: lil-414640

ABSTRACT

OBJETIVOS: O objetivo deste estudo foi à padronização de uma técnica de coleta e coloração em meio líquido que alie a praticidade e a riqueza citológica, possibilitando uma maior reprodutividade e facilidade microscópica. MÉTODOS: Ratas wistar (n=20) foram submetidas à coleta vaginal diária em salina e o lavado fixado (éter/álcool) e corado em suspensão com solução de azul de Evans 0,025 por cento. A amostra foi concentrada por centrifugação e observado sob objetiva de 40 x. RESULTADOS: Os esfregaços corados permitiram nítida diferenciação das fases do ciclo hormonal (diestro, proestro, estro e metaestro); além da diferenciação dos tipos celulares em relação ao seu grau de maturação tendo como parâmetros o tamanho celular, relação núcleo / citoplasma (RNC) e reação tintorial. O estudo demonstrou a existência de três padrões celulares básicos: células com baixa RNC, acentuada cianofília e pequeno tamanho; células com acréscimo na RNC, perda de cianofilia e maior volume citoplasmático e células queratinizadas anucleadas em aspecto de escama. CONCLUSÃO: A coloração do material permitiu, além da classificação citológica, a possibilidade de quantificação o que resultaria em um acompanhamento mais acurado do ciclo estral.


Subject(s)
Animals , Female , Rats , Estrous Cycle/physiology , Vagina/cytology , Vaginal Smears/methods , Cell Count , Cell Size , Coloring Agents , Cytological Techniques/methods , Cytological Techniques/standards , Evans Blue , Models, Animal , Rats, Wistar , Reproducibility of Results
5.
J. bras. patol. med. lab ; 39(3): 219-221, jul.-set. 2003. tab
Article in Portuguese | LILACS | ID: lil-349005

ABSTRACT

Entre as estratégias de controle de qualidade, a revisäo de exames negativos anteriores em pacientes com exame atual positivo é considerada uma das mais eficientes para detecçäo de falsos negativos. No presente estudo foram revistos os esfregaços prévios negativos de pacientes com NIC 2 ou NIC 3 nos anos de 1998 e 1999. Durante este período o Instituto Adolfo Lutz realizou 113.619 exames colpocitológicos, sendo 233 casos diagnosticados como NIC 2 ou NIC 3. Entre estes casos apenas 36 tinham exames prévios no Instituto Adolfo Lutz no período de 36 meses antes do diagnóstico original. Obtivemos 22 casos de NIC (12 NIC 1, sete NIC 2 e três NIC 3) e 14 casos com diagnóstico negativo (normal ou inflamatório), os quais pertenciam a 12 pacientes. Após revisäo destes esfregaços obtivemos os seguintes resultados: insatisfatório (dois casos), inflamatório (cinco casos), NIC 1 (três casos), NIC 2 (um caso). Concluindo, a revisäo dos exames anteriores negativos é uma estratégia altamente eficiente para detectar os resultados falsos negativos. Sua prática pode ser útil para orientar diversas medidas de educaçäo continuada


Subject(s)
Humans , Female , Cervical Intraepithelial Neoplasia , Vaginal Smears/methods , Vaginal Smears/standards , Gynecology , Quality Control , Cytological Techniques/standards
6.
Bangladesh Med Res Counc Bull ; 2001 Apr; 27(1): 23-32
Article in English | IMSEAR | ID: sea-156

ABSTRACT

Urinary cytology, ultrasonogram of the urinary bladder and cystoscopy were done in 99 cases of urinary bladder cancer patients in the department of urology of Dhaka Medical College Hospital (DMCH), Dhaka, during the period from January 1997 to December 1998. Pre transurethral resection of bladder tumour (TURBT), urinary cytology was positive in 20.20% cases and ultrasound scan was positive in 96.9% cases. Histopathological examination revealed 95.96% as transitional cell carcinoma:- Grade I (3.0%), Grade II (62.6%) and Grade III (34.4%). During follow-up urinary cytology was positive in only one case whereas ultrasound scan was positive in 53.5% cases of whom only 21.6% patients had actual recurrent bladder tumours proved by cystoscopy. Statistical analysis of the three tests and histopathology showed significant variation though by Chi-square test the ultrasound scan is found superior to urinary cytology. We conclude when ultrasonography of the urinary bladder shows any suspected lesion it should be examined by cystoscope and biopsy should be taken from the suspected area for confirmation. Neither urinary cytology nor ultrasound scan can replace cystoscopic examination.


Subject(s)
Adult , Aged , Aged, 80 and over , Carcinoma, Transitional Cell/pathology , Chi-Square Distribution , Combined Modality Therapy , Cystoscopy/standards , Cytological Techniques/standards , Female , Humans , Male , Middle Aged , Neoplasm Recurrence, Local/pathology , Neoplasm Staging , Patient Selection , Prospective Studies , Sensitivity and Specificity , Ultrasonography/standards , Urinary Bladder Neoplasms/pathology
7.
Braz. j. morphol. sci ; 16(1): 105-111, jan.-jun. 1999. ilus
Article in English | LILACS | ID: lil-303469

ABSTRACT

The pineal gland of Akodon arviculoides cursor shows a saccular pattern of organization with a wide and irregular lumen outlined by cuboidal and columnar epithelial cells. This feature was never reported before, in literature, among mammals. the pinealocytes are clustered together in the periphery of the organ and can be distinguished as light and dark cells. These cells show great amounts of mitochondria, microtubules, polysomes and a few dense-core vesicles. Higher amounts of dense-core vesicles are present in pinealocytes of young animals than in adult ones. In spite of the sacccular and follicular patterns of organization, that are considered a more primitive aspect of the gland, we could not observe, in this species, outer segments in the pinealocytes, which characterize the photoreceptor cells of the pineal organ in lower vertebrates.


Subject(s)
Animals , Adult , Epithelioid Cells/cytology , Cytoplasm , Pineal Gland , Cytological Techniques/standards
8.
Rev. Fac. Odontol. Bauru ; 6(1): 67-70, jan.-mar. 1998. ilus, tab
Article in Portuguese | LILACS, BBO | ID: lil-230051

ABSTRACT

Os autores apresentam com o intuito de divulgaçäo, um método para se estimar o grau de precisäo na avaliaçäo morfométrica do número absoluto de células em um orgäo. Este método permitirá ao pesquisador durante a fase de planejamento do projeto de pesquisa, calcular à partir de contagens iniciais, o tamanho da amostra (número total de campos histológicos) necessário para se trabalhar com um nível pré-estabelecido de erro nas avaliaçöes. Este fato é muito importante porque, às vezes, dependendo do objetivo do trabalho, um grau muito alto de precisäo pode näo ser necessário, nesse caso, estabelecendo-se um coeficiente de variaçäo aceitável, ocorrerá um enorme ganho no consumo de tempo


Subject(s)
Animals , Male , Female , Mice , Cell Count/methods , Submandibular Gland/cytology , Research Design/standards , Sampling Studies , Cytological Techniques/classification , Cytological Techniques/standards , Histological Techniques/standards
11.
Gac. méd. Méx ; 132(5): 507-13, sept.-oct. 1996.
Article in Spanish | LILACS | ID: lil-202945

ABSTRACT

La inmunología de las mucosas ha adquirido gran importancia, principalmente por la necesidad de comprender mejor la manera de estimular respuestas inmunes que prevengan enfermedades. En México, donde se reconoce la importancia de las enfermedades infecciosas que afectan mucosas (diarreas, neumonías, parasitosis) y existe un aumento preocupante de hipersensibilidades (asma, intolerancia alimentaria) no hay suficientes grupos dedicados a la investigación en esta área. El objetívo principal de los mecanismos de defensa de las mucosas es impedir la entrada del antígeno (Ag) (exclusión inmune) y evitar respuestas sistémicas indeseables (hipersensibilidad, autoinmunidad), por ejemplo contra Ags de la dieta. Una cantidad considerable de parásitos tienen como blanco o emplean las mucosas del organismo en alguna fase de su vida. A pesar de su indudable importancia, la inmunología de las mucosas en las infecciones parasitarias no ha sido estudiada en profundidad. Solo algunos estudios han abordado este tema usando animales de laboratorio y, afortunadamente, en los últimos años tienden a incrementarse. En el CINVESTAV, se estudia la inmunidad de las mucosas empleando un enfoque multidisciplinario, en proyectos que involucran parásitos como Entamoeba histolytica, Giardia lamblia y Trichinella spiralis. Tales estudios utilizan metodologías de vanguardia que se describen brevemente en este artículo.


Subject(s)
Defense Mechanisms , In Vitro Techniques , Mucous Membrane/immunology , Parasitology , Parasitology , Polymerase Chain Reaction , Cytological Techniques/standards , Immunologic Techniques/standards
12.
Rev. mex. reumatol ; 11(4): 142-7, jul.-ago. 1996. tab, ilus
Article in Spanish | LILACS | ID: lil-208149

ABSTRACT

Ro es un complejo molecular de tres ribonucleoproteínas de 60, 54 y 52 kDa de las que la isoforma de 60 kDa está asociada a uno o varios hYRNAs; se sabe que su ensamble ocurre en el núcleo y en forma de complejo Ro/hYRNA es rápidamente exportado al citoplasma. Este trabajo fue diseñado para determinar si la unión de hYRNA es indispensable para la permanencia nuclear de la proteína Ro de 60 kDa. La interacción entre Ro y hYRNA fue estudiada por inmunofluorescencia y Western blot usando células HEp-2 y anticuerpos anti-Ro. La ribonucleoproteína Ro se localizó en los compartimientos núcleo-citoplásmicos con un patrón granular fino. Para definir si la presencia de hYRNA era esencial en la localización nuclear de Ro se realizaron experimentos adicionales de fluorescencia en células digeridas con RNAasa o tripsina con los siguientes hallazgos. La proteína Ro puede detectarse en el núcleo en ausencia de los hYRNAs, en tanto que su presencia citoplásmica necesariamente está ligada a los hYRNA, ya que la digestión con RNAasa abolió su expresión en citoplasma. El patrón de fluorescencia citoplásmico fue restaurado al reconstituír las células con extractos celulares ricos en hYRNAs. La digestión con tripsina eliminó por completo los gránulos núcleo-citoplásmicos de Ro. Se puede concluir que existen dos pooles nucleares de ribonucleoproteína Ro de 60 kDa, libre y en complejo, en tanto que en el citoplasma solamente existe formando complejos con los hYRNAs


Subject(s)
Blotting, Western , Cell Physiological Phenomena , In Vitro Techniques , Lupus Erythematosus, Systemic/immunology , Molecular Biology , Ribonucleoproteins/physiology , Fluorescent Antibody Technique/standards , Cytological Techniques/standards
13.
Rev. mex. reumatol ; 11(2): 42-51, mar.-abr. 1996. tab, ilus
Article in Spanish | LILACS | ID: lil-208135

ABSTRACT

Los antígenos de diferenciación leucocitaria son moléculas que se detectan en la membrana de las células derivadas de la médula ósea y que se expresan con un patrón característico en cada una de las subpoblaciones de leucocitos, en sus variados estadios de diferenciación celular. En la práctica diaria, los antígenos de diferenciación leucocitaria son detectados con el empleo de anticuerpos monoclonales de origen murino; con el uso de diversas técnicas de laboratorio y estos anticuerpos, es factible el detectar, cuantificar, aislar y eliminar subpoblaciones definidas de células, tanto in vivo como in vitro. El conocimiento actual de los antígenos de diferenciación leucocitaria ha tenido un gran impacto en el campo de la reumatología, ya que ha facilitado grandemente el estudio de la fisiopatología de diversas enfermedades reumáticas, principalmente las de origen autoinmune. Además, los anticuerpos dirigidos en contra de los antígenos de diferenciación leucocitaria pueden ser de gran utilidad en el seguimiento y terapia de diversas condiciones caracterizadas por la presencia de inflamación y daño a tejidos


Subject(s)
Antibodies, Monoclonal , Antigens, CD/physiology , Rheumatic Diseases/physiopathology , Membrane Glycoproteins/analysis , Immune System/cytology , Leukocytes/physiology , Cytological Techniques/standards
14.
Rev. ADM ; 53(2): 86-9, mar.-abr. 1996. ilus, tab
Article in Spanish | LILACS | ID: lil-175531

ABSTRACT

Se realiza un estudio prospectivo en 30 pacientes los cuales fueron sometidos a citología exfoliativa y biopsia en lesiones de la cavidad bucal, obteniéndose resultados significativos ya que el estudio histológico corroboró el diagnóstico citológico previo, en 11 casos positivos de carcinoma epidermoide


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Biopsy , Mouth Neoplasms/diagnosis , Cytological Techniques/standards , Carcinoma, Squamous Cell/diagnosis , Prospective Studies
15.
Arch. med. res ; 27(2): 123-6, 1996. ilus, tab
Article in English | LILACS | ID: lil-200303

ABSTRACT

The variation in mechanical stress to which the aortic wall is subjected requires that forces be transmited between its components by means of relatively strong but compliant attachments. We have used transmission electron microscopy in order to study the cell to stroma contacts (smooth muscle cell-elastic fiber contact) in the tunica media of normotensive and hypertensive aortas of Sprague-Dawley rats. Hypertension was produced with a silver clip positioned around the left renal artery and the vessels were fexed by intravital perfusion at normal and elevated pressure. In ultrathin sections, the density of cell to stroma contacts per 100 µm cell perimeter and per 100 cell profiles were determined using an image analysis computer. In the hypertensive group the density of cell to stroma contacts fell considerably when compared with the control group. This research provides insights into the conditions under which high blood pressure may produce medial injuries and, perhaps, be a factor in the precipitation of dissections


Subject(s)
Rats , Animals , Aorta, Thoracic/physiology , Stromal Cells/cytology , Stress, Psychological/etiology , Hypertension/etiology , Rats/blood , Cytological Techniques/standards , Tunica Media/cytology
16.
Arch. med. res ; 27(3): 311-8, 1996. ilus, tab
Article in English | LILACS | ID: lil-200328

ABSTRACT

This quantitative ultrastructural survery of E. histolytica locomotion in Boyden chambers supports the concept that this parasitis is capable of random, chemokinetic and chemotactic motility. An E. histolytica committed to chemotaxis will flatten over the filter, accumulate smaller vacuoles at the front of the cell, and will also project pseudopods and its polarized body towards and alongside the chemoattractant axis, respectively. Other cell features such as cell polarization, membrane ruffling, hyaline, total number of pseudopods and caudal displacement of the nucleus appear to be associated with the locomotion efforts as such, perhaps reflecting speed (chemokinesis) but irrespective of orientation (chemotaxis). Finally, only on of the 11 features that were analyzed (i.e., number of vacuoles) failed to be distinctly associated with any of the movement forms studied. E. histolytica appears to possess the full repertoire of locomotion modalities observed in free moving eukaryots, and its motility translates into ultrastructural landmarks that could be useful indicators of subcellular events related to locomotion


Subject(s)
Cell Movement/physiology , Chemotaxis/physiology , Entamoeba histolytica/ultrastructure , Cytological Techniques/standards
17.
Bol. Hosp. San Juan de Dios ; 40(3): 160-3, mayo-jun. 1993. tab
Article in Spanish | LILACS | ID: lil-119895

ABSTRACT

Se analizan en forma retrospectiva los resultados de los informes de citología cervical de 2.018 pacientes, de los cuales 1.009 casos de una consulta privada, del Sector Oriente de Santiago y 1.009 casos concurrentes al Consultorio Externo de Ginecología del Hospital San Juan de Dios. Se abordaron las siguientes variables; resultado de citología cervical (maligna o inflamatoria), edad, paridad, uso de métodos anticonceptivos (dispositivos intrauterinos o anticonceptivos hormonales). Se encontró una clara diferencia entre ambos grupos, teniendo el grupo socioeconómico alto una mayor incidencia de citología maligna 1,7% (17 casos) frente a un 0,1% registrado en la clientela hospitalaria (1 caso). En relación a estos 17 casos, se evidenció una alta proporción de estados incipientes (NIE I:82,4%); su promedio de edad fue de 34 años y a menor paridad hubo menor incidencia de citologías malignas. Se comprobó que el 71% de estas pacientes utilizaba métodos anticonceptivos pero no se encontró diferencia entre las usuarias de DIU y de anticonceptivos hormonales. La citología inflamatoria predominó en el grupo hospitalario 62,8% (n=635) frente a un 10,4% (n=109) en los casos de consulta privada


Subject(s)
Humans , Female , Adolescent , Adult , Cervix Uteri/cytology , Cytological Techniques/standards , Vaginal Smears , Contraception/methods , Socioeconomic Factors , Uterine Cervical Diseases/epidemiology
18.
Article in English | IMSEAR | ID: sea-38481

ABSTRACT

The diagnostic accuracy of the toluidine blue stain approximates that of the Papanicolaou stain in non-gynecologic cytology. In this study a sensitivity of 95.3 and 96.9 per cent was achieved by used of the toluidine blue and Papanicolaou stains respectively. The specificity for both stains was identical at 96.3 per cent. Given the high degree of accuracy achieved with the toluidine blue stain in this study, and given its numerous advantages over the Papanicolaou stain, it is suggested that consideration be given to using the toluidine blue stain routinely in non-gynecologic cytology to differentiate malignancy from benignancy.


Subject(s)
Cytological Techniques/standards , Evaluation Studies as Topic , Female , Hematoxylin/diagnosis , Humans , Neoplasms/epidemiology , Sensitivity and Specificity , Tolonium Chloride/diagnosis , Vaginal Smears/standards
19.
J Postgrad Med ; 1990 Oct; 36(4): 194-8
Article in English | IMSEAR | ID: sea-117810

ABSTRACT

Comparative evaluation of bone marrow aspirate particle smears, imprints and biopsy sections was done on 30 haematological problems. Core needle biopsy of the bone marrow is a safe and useful procedure. It is a valuable diagnostic aid for measurement of marrow cellularity, metastatic tumours and fibrosis. It should not be taken as a substitute for examination of the marrow by aspiration smear but is a complementary procedure which affords several advantages. Bone marrow biopsy was of maximum utility in myelofibrosis which was diagnosed on biopsy alone. There were three additional cases with normal bone marrow aspiration in which specific diagnosis could only be made from bone marrow biopsy sections. New methodologies i.e. plastic embedding and semi thin sections of undecalcified bone marrow, can be expected to improve the cytological details of tissue obtained by biopsy. Imprint preparations obtained from biopsy can be useful in patients of malignancy but we have found them to be of limited value except in cases of dry tap.


Subject(s)
Biopsy, Needle/methods , Bone Marrow Examination/methods , Cytological Techniques/standards , Evaluation Studies as Topic , Hematologic Diseases/diagnosis , Hospitals, University , Humans , India/epidemiology , Sensitivity and Specificity
20.
J Indian Med Assoc ; 1989 May; 87(5): 108-10
Article in English | IMSEAR | ID: sea-105824

ABSTRACT

Fine needle aspiration biopsy cytology of salivary gland tumours was performed in 97 patients. Histological confirmation was available in all cases except 9 cases of sialo-adenitis which responded to antibiotics. Accuracy of cytological diagnosis in exact categorisation of benign and malignant tumours was 93.7% and 91.1% respectively. False negative was 4.1%. The overall accuracy was 95.8%. There was no false positive report. Exact classification of tumour was made in 94.1% cases, ie, 80 out of 85 tumours. No complication was encountered in this procedure.


Subject(s)
Adolescent , Adult , Aged , Biopsy, Needle , Cytological Techniques/standards , False Negative Reactions , Female , Histological Techniques/standards , Humans , Male , Middle Aged , Salivary Gland Neoplasms/diagnosis
SELECTION OF CITATIONS
SEARCH DETAIL