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2.
Rev. cuba. med ; 60(3): e2029, 2021.
Article in Spanish | LILACS, CUMED | ID: biblio-1347519

ABSTRACT

Introducción: El dengue es una enfermedad infecciosa causada por el virus del dengue. Es un problema complejo tanto por su magnitud como por los elementos que hay que tener presentes para su control. En la situación actual de nuestra Región, esta realidad constituye un reto para el control del mosquito vector, y, por tanto, de la enfermedad. Objetivo: Caracterizar a esta enfermedad y demostrar la necesidad de realizar acciones comunitarias para control del dengue y su prevención. Métodos: Se realizó una revisión sistemática de documentos de sociedades científicas dedicadas a la Epidemiologia en la Biblioteca Virtual de Salud (BVS) con límite de fecha de abril de 2015 a abril de 2020, e incluyendo artículos tanto en inglés como en español. Se localizaron 262 estudios finalmente se seleccionaron 20. Conclusiones: En el futuro se espera la aparición de cepas productoras de una mayor viremia por la diversidad genética del virus, con casos clínicos más complicados; se está ante una enfermedad que ofrece un reto a los médicos, de ahí la importancia de trabajar en su prevención para evitar la propagación de la enfermedad en nuestra población(AU)


Introduction: Dengue is an infectious disease caused by the dengue virus. It is a complex problem both because of its magnitude and because of the elements that must be taken into account to its control. In our region current situation, this reality constitutes a challenge for the control of the mosquito vector, and therefore, of the disease. Objective: To describe this disease and to demonstrate the need for community actions to control dengue and its prevention. Methods: A systematic review of documents from scientific societies dedicated to Epidemiology was carried out in the Virtual Health Library (VHL) with a date limit from April 2015 to April 2020, and including articles in both English and Spanish. Two hundred sixty-two studies were located, finally 20 were selected. Conclusions: In the future, the appearance of strains producing higher viremia due to the genetic diversity of the virus is expected, with more complicated clinical cases. We are facing a disease that offers a challenge to doctors, hence the importance of working on its prevention to avoid the spread of the disease in our population(AU)


Subject(s)
Humans , Aedes , Vector Control , Dengue/prevention & control , Dengue Virus
3.
Rev. cuba. med. trop ; 73(2): e551, 2021. tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1347496

ABSTRACT

El dengue es la infección trasmitida por vectores con mayor impacto en carga de enfermedad, económica y social a nivel mundial, con más de 3,6 billones de personas en riesgo de infección. Sus manifestaciones son variables, caracterizadas en su mayoría por síndrome febril con riesgo de sangrado, choque y muerte. El compromiso pulmonar es infrecuente, siendo el síndrome de dificultad respiratoria aguda una complicación inesperada, aunque informada, asociada a un mal pronóstico. Se presenta un paciente sin antecedentes relevantes de importancia, con focalización pulmonar severa asociado a infección por el virus dengue. En el caso presentado se descartaron procesos infecciosos bacterianos u otros agentes causales de síndrome de dificultad respiratoria aguda, lo que sumado a las características clínicas de ingreso, zona de ocurrencia del caso considerada como endémica, curso clínico, paraclínico y la franca positividad de las pruebas para dengue así como la seroconversión de estas, a pesar de no haber sido realizadas pruebas moleculares, se consideró como el agente causal más probable el virus dengue. Todo esto lleva a recomendar siempre considerarlo como potencial agente causal, lo que permite así un diagnóstico y manejo óptimos(AU)


Dengue is the vector-borne infection with the greatest impact on disease, economic and social burden worldwide, with more than 3.6 billion people under risk of contagion. Its manifestations are varied, most of them characterized by febrile syndrome with a risk of bleeding, shock and death. Pulmonary involvement is infrequent, and acute respiratory distress syndrome is an unexpected complication, though it has been reported in association to a bad prognosis. A case is presented of a male patient without relevant antecedents of interest, with severe pulmonary focalization associated to dengue virus infection. Bacterial infectious processes and other causative agents of acute respiratory distress syndrome were ruled out. In view of the patient's clinical status at admission, the endemicity of the patient's area of residence, the clinical and paraclinical course, and the obvious positivity of the dengue tests performed and their seroconversion, despite not having conducted molecular tests, it was concluded that the most probable causative agent was dengue virus. Therefore, it is recommended that dengue infection always be considered as a potential causative agent of acute respiratory distress syndrome, thus contributing to optimal diagnosis and management(AU)


Subject(s)
Humans , Male , Adult , Respiratory Distress Syndrome, Newborn , Endemic Diseases , Molecular Diagnostic Techniques , Dengue Virus , Prognosis , Cost of Illness , Seroconversion
4.
Biomédica (Bogotá) ; 41(2): 247-259, abr.-jun. 2021. tab, graf
Article in English | LILACS | ID: biblio-1339264

ABSTRACT

Abstract | Introduction: Arthropod-borne viruses (arboviruses) cause morbidity and mortality in humans and domestic animals worldwide. The percentage of population immunity or susceptibility to these viruses in Ecuador is unknown. Objectives: To investigate the proportion of Ecuadorian populations with IgG antibodies (Abs) (past exposure/immunity) and IgM Abs (current exposure) against flaviviruses and alphaviruses and to study the activity of these viruses in Ecuador. Materials and methods: During 2009-2011, we conducted a serosurvey for selected arboviruses in humans (n=1,842), equines (n=149), and sentinel hamsters (n=84) at two coastal locations and one in the Amazon basin (Eastern Ecuador) using enzyme-linked immunosorbent assay and hemagglutination inhibition test. Results: From 20.63% to 63.61% of humans showed IgG-antibodies for the flaviviruses: Dengue virus (DENV), yellow fever virus (YFV) Saint Louis encephalitis virus, and West Nile virus (WNV); from 4.67% to 8.63% showed IgG-Abs for the alphaviruses: Venezuelan equine encephalitis virus, eastern equine encephalitis virus, and western equine encephalitis virus. IgM-Abs were found for DENV and WNV. Equines and hamsters showed antibodies to alphaviruses in all locations; two hamsters seroconverted to YFV in the Amazonia. Conclusions: The results show a YFV vaccination history and suggest the activity of arboviruses not included in the current surveillance scheme. Enhanced arbovirus and mosquito surveillance, as well as continued YFV vaccination and evaluation of its coverage/ effectiveness, are recommended.


Resumen | Introducción. Los virus transmitidos por artrópodos (arbovirus) causan morbilidad y mortalidad en humanos y animales domésticos mundialmente. Se desconoce el porcentaje de inmunidad o vulnerabilidad de la población ecuatoriana ante estos virus. Objetivos. Investigar la proporción de poblaciones ecuatorianas con anticuerpos IgG (exposición o inmunidad pasada) y anticuerpos IgM (exposición reciente) contra flavivirus y alfavirus, e investigar su actividad en Ecuador. Materiales y métodos. Entre 2009 y 2011, se llevó a cabo una encuesta serológica para arbovirus en humanos (n=1.842), equinos (n=149) y hámsters centinela (n=84) en dos localidades costeras y en una en la Amazonía, utilizando la prueba ELISA (Enzyme-Linked ImmunoSorbent Assay) y la prueba de inhibición de la hemaglutinación. Resultados. Entre el 20,63 y el 63,61 % de los humanos registraron IgG contra el virus del dengue (DENV), el de la fiebre amarilla (YFV), el de la encefalitis de San Luis y el del Nilo Occidental (WNV); entre 4,67 y 8,63 % tenían IgG para los virus de la encefalitis equina venezolana, de la encefalitis equina del este y de la encefalitis equina del oeste. Se encontró IgM para DENV y WNV. En los equinos y en los hámsters se encontraron anticuerpos contra alfavirus en todas las localidades muestreadas; dos hámsters mostraron seroconversión a YFV en la Amazonía. Conclusiones. Los resultados del estudio evidenciaron los antecedentes de vacunación contra el YFV y sugieren la actividad de arbovirus no incluidos en el esquema de vigilancia actual. Se recomienda ampliar la vigilancia de arbovirus y mosquitos, continuar con la vacunación contra el YFV, y evaluar su cobertura y efectividad.


Subject(s)
Arboviruses , West Nile virus , Yellow fever virus , Dengue Virus , Encephalitis Virus, Eastern Equine , Encephalitis Virus, Venezuelan Equine
6.
Rev. cuba. med. trop ; 73(1): e573, tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1280324

ABSTRACT

. Introducción: El dengue es la enfermedad viral transmitida por mosquitos de más rápida propagación en el mundo. A pesar de la alta incidencia de la enfermedad no existe tratamiento antiviral efectivo ni vacuna aprobada para su uso contra esta infección. La planta Ageratina havanensis ha sido ampliamente utilizada por nuestra cultura popular y en otros países debido a sus diversas propiedades biológicas. Objetivo: Evaluar la actividad antiviral de cuatro extractos provenientes de Ageratina havanensis y dos flavonoides aislados de dicha planta frente al virus dengue 2. Métodos: La pesquisa primaria de actividad antiviral de cada extracto y flavonoide se realizó mediante el ensayo de detección de la productividad vírica, y se evaluó la acción virucida de los extractos etanólico de tallo y butanólico de hoja. Resultados: Todos los extractos inhibieron la replicación viral al evidenciarse disminución del número de placas de lisis, sin embargo, no se evidenció disminución del título infectivo viral en el ensayo virucida. El tratamiento con la sakuranetina mostró valores de inhibición de la productividad viral entre 61-91 por ciento, y para la 7-metoxiaromadendrina se registraron valores de reducción viral entre 64-86 por ciento. Conclusiones: Los extractos y flavonoides aislados de Ageratina havanensis mostraron acción antiviral. Los extractos etanólico de tallo y butanólico de hojas no poseen acción virucida sobre la cepa viral empleada. El presente trabajo constituye el primer informe respecto a la evaluación de extractos y compuestos aislados de A. havanensis frente al virus dengue(AU)


Introduction: Dengue is the fastest spreading mosquito-borne viral disease worldwide. Despite its high incidence, no effective antiviral treatment or vaccine has been approved for use against this infection. The plant Ageratina havanensis has been widely used in our folk culture and in other countries due to its various biological properties. Objective: Evaluate the antiviral activity of four extracts from Ageratina havanensis and two flavonoids isolated from that plant against the dengue 2 virus. Methods: Primary screening of the antiviral activity of each extract and flavonoid was conducted by viral productivity detection assay, and an evaluation was carried out of the virucidal action of the stem ethanolic extract and the leaf butanolic extract. Results: All the extracts inhibited viral replication by a reduction in the number of lysis plates. However, no evidence was found of a reduced viral infective titer in the virucidal assay. Treatment with sakuranetin achieved viral productivity inhibition values of 61 percent-91 percent, whereas 7-methoxyaromadendrin obtained viral reduction values of 64 percent-86 percent Conclusions: The extracts and flavonoids isolated from Ageratina havanensis displayed antiviral activity. The stem ethanolic extract and the leaf butanolic extract do not have a virucidal action against the viral strain used. The paper is the first report about the effect of extracts and compounds isolated from A. havanensis against the dengue virus(AU)


Subject(s)
Humans , Microscopy, Electron, Scanning Transmission , Ageratina , Dengue , Dengue Virus
8.
Biomédica (Bogotá) ; 41(1): 161-167, ene.-mar. 2021. tab, graf
Article in English | LILACS | ID: biblio-1249068

ABSTRACT

Abstract | Introduction: It is essential to determine the interactions between viruses and mosquitoes to diminish dengue viral transmission. These interactions constitute a very complex system of highly regulated pathways known as the innate immune system of the mosquito, which produces antimicrobial peptides that act as effector molecules against bacterial and fungal infections. There is less information about such effects on virus infections. Objective: To determine the expression of two antimicrobial peptide genes, defensin A and cecropin A, in Aedes aegypti mosquitoes infected with DENV-1. Materials and methods: We used the F1 generation of mosquitoes orally infected with DENV-1 and real-time PCR analysis to determine whether the defensin A and cecropin A genes played a role in controlling DENV-1 replication in Ae. aegypti. As a reference, we conducted similar experiments with the bacteria Escherichia coli. Results: Basal levels of defensin A and cecropin A mRNA were expressed in uninfected mosquitoes at different times post-blood feeding. The infected mosquitoes experienced reduced expression of these mRNA by at least eightfold when compared to uninfected control mosquitoes at all times post-infection. In contrast with the behavior of DENV-1, results showed that bacterial infection produced up-regulation of defensin and cecropin genes; however, the induction of transcripts occurred at later times (15 days). Conclusion: DENV-1 virus inhibited the expression of defensin A and cecropin A genes in a wild Ae. aegypti population from Venezuela.


Resumen | Introducción. Es esencial determinar las interacciones entre los virus y los mosquitos para disminuir la transmisión viral. Estas interacciones constituyen un sistema muy complejo y muy regulado conocido como sistema inmunitario innato del mosquito, el cual produce péptidos antimicrobianos, moléculas efectoras que funcionan contra las infecciones bacterianas y fúngicas; se tiene poca información de su acción sobre los virus. Objetivo. Determinar la expresión de dos genes AMP (defensina A y cecropina A) en mosquitos Aedes aegypti infectados con el virus DENV-1. Materiales y métodos. Se infectaron oralmente mosquitos de generación F1 con DENV-1 y mediante el análisis con PCR en tiempo real se determinó el potencial papel de los genes defensina A y cecropina A en el control de la replicación del DENV-1 en Ae. aegypti. Como referencia, se infectaron mosquitos con Escherichia coli. Resultados: Los mosquitos no infectados expresaron niveles basales de los ARNm de los genes defensina A y cecropina A en diversos momentos después de la alimentación. Los mosquitos infectados experimentaron una reducción, por lo menos, de ocho veces en la expresión de estos ARNm con respecto a los mosquitos de control en todo el periodo posterior a la alimentación. En contraste con el comportamiento del virus DENV-1, los resultados mostraron que la infección bacteriana produjo una regulación positiva de los genes defensina y cecropina; sin embargo, la inducción de los transcritos ocurrió tardíamente (15 días). Conclusión. El virus DENV-1 inhibió la expresión de los genes defensina A y cecropina A en una población silvestre de Ae. aegypti en Venezuela.


Subject(s)
Aedes , Dengue Virus , alpha-Defensins , Escherichia coli , Cecropins
9.
Cienc. Salud (St. Domingo) ; 5(2): [ 57-67], Ene-Abr. 2021. tab
Article in Spanish | LILACS | ID: biblio-1358711

ABSTRACT

Introducción: Aedes spp. y la dinámica del virus del dengue está altamente influenciada por factores ambientales. Una relación detallada entre el clima y la enfermedad en los períodos inter e intra-epidémicos podrían beneficiar la vigilancia del dengue para optimizar la preparación y las políticas adecuadas de control de vectores. Métodos: se analizaron los informes de casos de dengue y las variables climáticas en Santo Domingo, República Dominicana, para determinar la correlación del período 2012- 2018 y los diferentes tiempos de retraso. Se llevó a cabo un análisis de regresión de dichas variables para comprender mejor las relaciones entre las tasas de incidencia del dengue y los cambios climáticos. Resultados: durante los brotes epidémicos, la temperatura (r = 0.73, p <0.001) y la humedad relativa (r = -0.2, p = 0.009) se correlacionan significativamente con la incidencia del dengue con un retraso de 9 semanas, el análisis de regresión muestra que la temperatura media (b = 62.401, p < 0.001), precipitación (b = 2.810, p <0.001) y humedad relativa (b = -5.462, p = 0.025) fueron predictores significativos. Durante los períodos inter-epidémico, la temperatura (r = 0.23, p <0.001) tuvo una correlación significativa con la incidencia del dengue con un retraso de 7 semanas, la humedad relativa (b = 1.454, p <0.05) y la temperatura media (b = 5.14, p <0.01) son predictores significativos de la cantidad de casos de dengue. La precipitación no se correlacionó significativamente con la incidencia del dengue. Conclusiones: existe una relación no lineal entre los factores climáticos y la incidencia del dengue. La infección por dengue depende del clima, y la temperatura parece jugar un papel importante en los factores climáticos.


Introduction: Aedes spp. and Dengue Virus dynamics are highly influenced by environmental factors. A detailed relationship between climate and disease in inter and intra-epidemic periods may benefit dengue surveillance, preparedness, and adequate vector control policies. Methods: Dengue case reports and climatic variables in Santo Domingo, Dominican Republic, were analyzed for climate variables correlation from the period 2012-2018 and varying lag times. Regression analysis of climatic variables was carried out to better understand significant correlations between dengue incidence rates and changes in climate. Results: During epidemic outbreaks, temperature (r = 0.73, p < 0.001) and relative humidity (r = -0.22, p = 0.009) demonstrated a significant correlation with dengue incidence. Our regression analysis demonstrates an increase 62.4 cases for each degree Celsius increased with a 9-week-lag. Regression analysis also demonstrated mean temperature (b= 62.401, p < 0.001), precipitation (b = 2.810, p < 0.001), and relative humidity (b = -5.462, p = 0.025) to be significant predictors. During inter-epidemic periods, temperature (r = 0.23, p < 0.001) had a significant correlation with dengue incidence with a 7-week-lag, which demonstrates that relative humidity (b = 1.454, p < 0.05), and mean temperature (b = 5.14, p < 0.01) are significant predictors of the quantity of dengue cases. Precipitation did not significantly correlate with dengue incidence. Conclusions: A non-linear relationship between climatic factors and dengue incidence exists in the Dominican Republic. Dengue infection is climate-dependent and temperature seems to play a significant role in climatic factors


Subject(s)
Disease Transmission, Infectious , Dengue Virus , Climate , Dominican Republic
10.
Braz. j. biol ; 81(2): 237-240, 2021. tab
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1153345

ABSTRACT

Dengue is a viral disease which is serious health concern from last few decades and the infection transmitted through mosquito bite into human. This study was conducted to carry out prevalence of dengue fever in District Swabi. A total of 196 blood sample were collected from patients with age ranges (0-80 years) having dengue fever on the basis of physical symptoms from Bacha Khan Medical Complex Swabi during August to October 2017. Serological test were performed for detection of IgM, IgG and NS1 (Non structural protein antigen of virus) against dengue. Out of total 196 confirmed dengue cases the most prone gender was male 123(62%) while 73(38%) were female. Among the age groups; 21-30 years group 62 (31.6%) was found the most predominated age group. The higly affected areas in district Swabi were Topi (40.8%) followed by Main Swabi (27%), Maneri (8.2%), Marghuz (6%), Shawa Ada (5.1%), Shah Mansoor (5.1%), Gohati (4.1%), and Chota Lahore (3.6%). Therefore, the health department should take actions by educating the public about basic cleanliness of the environment. The community should be encouraged to participate in the control of such vector based diseases/infections.


A dengue é uma doença viral que é um sério problema de saúde das últimas décadas e a infecção transmitida através da picada de mosquito em humanos. Este estudo foi realizado para realizar a prevalência da dengue no distrito de Swabi. Foram coletadas 196 amostras de sangue de pacientes com faixa etária (0 a 80 anos) com dengue com base nos sintomas físicos do Complexo Médico Bacha Khan de Swabi, no período de agosto a outubro de 2017. Foram realizados testes sorológicos para detecção de IgM, IgG e NS1 (antígeno proteico não estrutural do vírus) contra a dengue. Do total de 196 casos confirmados de dengue, o sexo mais propenso foi o masculino 123 (62%), enquanto 73 (38%) eram do sexo feminino. Entre as faixas etárias; A faixa etária de 21 a 30 anos 62 (31,6%) foi a faixa etária mais predominante. As áreas altamente afetadas no distrito de Swabi foram Topi (40,8%), seguidas por Main Swabi (27%), Maneri (8,2%), Marghuz (6%), Shawa Ada (5,1%), Shah Mansoor (5,1%), Gohati (4,1%) e Chota Lahore (3,6%). Portanto, o departamento de saúde deve tomar ações educando o público sobre a limpeza básica do meio ambiente. A comunidade deve ser incentivada a participar do controle de tais doenças / infecções baseadas em vetores.


Subject(s)
Humans , Male , Female , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Dengue/epidemiology , Dengue Virus , Pakistan/epidemiology , Epidemiologic Studies , Disease Outbreaks
11.
Braz. j. med. biol. res ; 54(7): e10240, 2021. tab, graf
Article in English | LILACS | ID: biblio-1249316

ABSTRACT

Dengue is the most important arthropod-borne viral disease worldwide. Infection with any of the four dengue virus (DENV) serotypes can be asymptomatic or lead to disease with clinical symptoms ranging from undifferentiated and self-limiting fever to severe dengue disease, which can be fatal in some cases. Currently, no specific antiviral compound is available for treating DENV. The aim of this study was to identify compounds in plants from Paraguayan folk medicine with inhibitory effects against DENV. We found high virucidal activity (50% maximal effective concentration (EC50) value of 24.97 µg/mL) against DENV-2 in the ethanolic extract of the roots of Solanum sisymbriifolium Lam. (Solanaceae) without an evident cytotoxic effect on Vero E6 cells. Three saponins isolated from the root extract showed virucidal effects (EC50 values ranging from 24.9 to 35.1 µg/mL) against DENV-2. Additionally, the saponins showed inhibitory activity against yellow fever virus (EC50 values ranging from 126 to 302.6 µg/mL), the prototype virus of the Flavivirus genus, suggesting that they may also be effective against other members of this genus. Consequently, these saponins may be lead compounds for the development of antiviral agents.


Subject(s)
Saponins/pharmacology , Solanum , Dengue Virus , Antiviral Agents/pharmacology , Virus Replication , Yellow fever virus
12.
Cad. Saúde Pública (Online) ; 37(4): e00079620, 2021. tab, graf
Article in English | LILACS | ID: biblio-1285828

ABSTRACT

Around 14% of world dengue virus (DENV) cases occur in the Americas, most of them in Brazil. While socioeconomic, environmental, and behavioral correlates have been analyzed thoroughly, the role played by population mobility on DENV epidemics, especially at the local level, remains scarce. This study assesses whether the daily pattern of population mobility is associated with DENV incidence in Campinas, a Brazilian major city with over 1.2 million inhabitants in São Paulo State. DENV notifications from 2007 to 2015 were geocoded at street level (n = 114,884) and combined with sociodemographic and environmental data from the 2010 population census. Population mobility was extracted from the Origin-Destination Survey (ODS), carried out in 2011, and daily precipitation was obtained from satellite imagery. Multivariate zero-inflated negative binomial regression models were applied. High population mobility presented a relevant positive effect on higher risk for DENV incidence. High income and residence in apartments were found to be protective characteristics against the disease, while unpaved streets, number of strategic points (such as scrapyards and tire repair shops), and precipitation were consistently risk factors.


Cerca de 14% de todos os casos de dengue (DENV) ocorrem nas Américas, a maioria dos quais no Brasil. Os correlatos socioeconômicos, ambientais e comportamentais já foram analisados em profundidade, mas há pouco conhecimento, principalmente em nível local, sobre o papel da mobilidade populacional nas epidemias de DENV. O estudo pretende verificar se o padrão diário de mobilidade populacional está associado à incidência do DENV em Campinas, cidade brasileira com mais de 1,2 milhão de habitantes no Estado de São Paulo. As notificações de DENV entre 2007 e 2015 foram georreferenciadas em nível de logradouro (n = 114.884) e combinadas com dados sociodemográficos e ambientais do censo populacional de 2010. A mobilidade populacional foi extraída da Pesquisa de Origem/Destino (POD) realizada em 2011, e a pluviometria diária foi obtida através de imagens de satélite. Foram aplicados modelos de regressão multivariada com resposta binomial negativa inflacionados de zeros. A mobilidade populacional alta apresentou efeito positivo relevante sobre a incidência mais elevada de DENV. Renda alta e residência em apartamento mostrou efeito protetor contra a doença, enquanto ruas não pavimentadas, número de pontos críticos (p.ex.: ferros-velhos e borracharias) e pluviosidade alta apareceram enquanto fatores de risco.


Alrededor del 14% de los casos mundiales de virus dengue (DENV por sus siglas en inglés) se produce en las Américas, la mayoría de ellos en Brasil. Mientras que las correlaciones socioeconómicas, ambientales y de comportamiento se han analizado a fondo, el papel jugado por la movilidad de la población con epidemia de DENV, especialmente en un nivel local, continúa siendo escasa. Este estudio evalúa si el patrón diario de movilidad de población está asociado con la incidencia de DENV en Campinas, una gran ciudad brasileña con más de 1,2 millones de habitantes en el estado de São Paulo. Las notificaciones de DENV desde 2007 a 2015 fueron geocodificadas en un nivel de calle (n = 114,884), y combinadas con datos sociodemográficos, además de ambientales mediante el censo de población de 2010. La movilidad de la población se extrajo de la Encuesta Origen-Destino (ODS por sus siglas en inglés), llevada a cabo en 2011, la precipitación diaria se obtuvo mediante imágenes por satélite. Se aplicaron modelos de regresión binomial negativa multivariados con ceros inflados. La alta movilidad de la población presentó un efecto positivo relevante, respecto a un mayor riesgo en la incidencia por DENV. Contar con ingresos altos y residir en apartamentos resultaron ser factores protectores contra la enfermedad, mientras que las calles sin pavimentar, así como algunos puntos estratégicos como los desguaces y tiendas de reparación de ruedas, al igual que las precipitaciones fueron consistentemente factores de riesgo.


Subject(s)
Humans , Dengue/epidemiology , Dengue Virus , Epidemics , Brazil/epidemiology , Cities/epidemiology
13.
Evid. actual. práct. ambul ; 24(1): e002064, 2021. ilus, tab
Article in Spanish | LILACS | ID: biblio-1222368

ABSTRACT

El dengue es un problema creciente para la salud pública mundial. En Argentina, los casos se han ido incrementado en los últimos años. La vacuna Dengvaxia (CYD-TDV) fue aprobada por la Agencia Nacional de Medicamentos, Alimentos y Tecnología (ANMAT) en 2017, y actualmente está indicada para personas entre los 9 y 45 años de edad que residan enzonas endémicas. A partir de la consulta de una paciente sobre la posibilidad de vacunarse contra el dengue, la autora se plantea la pertinencia de su indicación, teniendo en cuenta la eficacia y seguridad de la vacuna. Luego de una búsqueda rápida se encontró evidencia que señala que la vacuna contra el dengue CYD-TDV mostró poca eficacia en comparación con otras vacunas disponibles en el mercado, siendo más segura y eficaz en personas que ya han sido infectadas anteriormente por el virus del dengue (sujetos seropositivos). En cambio, se observó un aumento del riesgo de dengue grave en los infectados por vez primera tras la vacunación (sujetos seronegativos). Se concluye que la estrategia recomendada consiste en vacunar únicamente a las personas que hayan tenido infección por dengue con anterioridad, consistiendo en una buena práctica la toma de decisiones compartidas con cada paciente. (AU)


Dengue is a growing problem for global public health. In Argentina, cases have been increasing in recent years. The Dengvaxia vaccine (CYD-TDV) was approved by the National Agency for Medicines, Food and Technology in 2017, and it is currently indicated for people between 9 and 45 years of age who reside in endemic areas. Based on the consultation of a patient about the possibility of being vaccinated against dengue, the author considers the relevance of its indication, taking into account the efficacy and safety of the vaccine. After a quick search, evidence was found that indicates that the CYD-TDV dengue vaccine showed little efficacy compared to other vaccines available on the market, being safer and more effective in people who have already been previously infected by the dengue virus (seropositive subjects). In contrast, an increased risk of severe dengue was observed in those infected for the first time after vaccination (seronegative subjects). It is concluded that the recommended strategy consists of vaccinating only people who have had dengue infection before, making shared decisions with each patient a good practice. (AU)


Subject(s)
Humans , Female , Adult , Dengue/immunology , Dengue Vaccines/pharmacology , Patient Participation , Meta-Analysis as Topic , Public Health , Severe Dengue/etiology , Dengue/prevention & control , Dengue Virus/classification , Dengue Vaccines/adverse effects , Dengue Vaccines/immunology , Systematic Reviews as Topic , Decision Making, Shared
14.
Rev. bras. epidemiol ; 24: e210020, 2021. tab, graf
Article in English | LILACS | ID: biblio-1156025

ABSTRACT

Objective: To describe the entry of Dengue virus (DENV) serotypes in Brazil and its federative units. Methods: A systematic review of studies published between 1980 and 2018 in databases and in the gray literature was performed using descriptors related to the years of entry of the DENV serotypes. Additionally, experts and official sources of information (Brazilian Ministry of Health) were consulted. Results: From 100 publications selected for the systematic review, 26 addressed the entry of DENV serotypes in the North region of the country, 33 in the Northeast, 24 in the Southeast, 14 in the Central-West, and five in the South. DENV-1 and DENV-4 were introduced in the North region in 1981. DENV-2 was introduced in the Southeast in 1990. DENV-3 was introduced in the North in 1999. Conclusion: The rapid expansion of dengue throughout the Brazilian territory was verified from the second half of the 1980s, with the gradual entry of the four serotypes, which resulted in the emergence of epidemics of arbovirus, which are currently verified in the country. Considering the epidemiology of the disease, more information should be disseminated and published in the wide-ranging scientific literature for a better understanding of the spread and circulation of DENV serotypes.


RESUMO: Objetivo: Descreveu-se a entrada de sorotipos do vírus da Dengue (DENV) no Brasil e em suas unidades federativas. Métodos: Realizou-se uma revisão sistemática de estudos publicados entre 1980 e 2018 em bancos de dados e na literatura cinzenta, utilizando-se descritores relacionados aos anos de entrada dos sorotipos do DENV. Além disso, consultou-se especialistas e fontes oficiais de informação (Ministério da Saúde do Brasil). Resultados: Das 100 publicações selecionadas para a revisão sistemática, 26 abordaram a entrada de sorotipos de DENV na região Norte do país, 33 no Nordeste, 24 no Sudeste, 14 no Centro-Oeste e cinco no Sul. O DENV-1 e o DENV-4 foram introduzidos na região Norte em 1981. O DENV-2 foi introduzido no Sudeste em 1990. O DENV-3 foi introduzido no Norte em 1999. Conclusão: A rápida expansão da dengue por todo o território brasileiro foi verificada a partir da segunda metade dos anos de 1980, com a entrada gradativa dos quatro sorotipos, o que resultou no surgimento de epidemias da arbovirose, que são atualmente verificadas no país. Considerando a epidemiologia da doença, mais informações devem ser divulgadas e publicadas na literatura científica de amplo alcance, para melhor entendimento da propagação e circulação dos sorotipos de DENV.


Subject(s)
Humans , Dengue/epidemiology , Dengue Virus/genetics , Brazil/epidemiology , Serogroup , Geography
15.
Mem. Inst. Oswaldo Cruz ; 116: e200547, 2021. tab, graf
Article in English | LILACS | ID: biblio-1250365

ABSTRACT

BACKGROUND Forty percent of the world's population live in areas where they are at risk from dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. Dengue viruses are transmitted primarily by the mosquito Aedes aegypti. In Cali, Colombia, approximately 30% of field collected Ae. aegypti are naturally refractory to all four dengue serotypes. OBJECTIVES Use RNA-sequencing to identify those genes that determine refractoriness in feral mosquitoes to dengue. This information can be used in gene editing strategies to reduce dengue transmission. METHODS We employed a full factorial design, analyzing differential gene expression across time (24, 36 and 48 h post bloodmeal), feeding treatment (blood or blood + dengue-2) and strain (susceptible or refractory). Sequences were aligned to the reference Ae. aegypti genome for identification, assembled to visualize transcript structure, and analyzed for dynamic gene expression changes. A variety of clustering techniques was used to identify the differentially expressed genes. FINDINGS We identified a subset of genes that likely assist dengue entry and replication in susceptible mosquitoes and contribute to vector competence. MAIN CONCLUSIONS The differential expression of specific genes by refractory and susceptible mosquitoes could determine the phenotype, and may be used to in gene editing strategies to reduce dengue transmission.


Subject(s)
Animals , Aedes , Dengue , Dengue Virus , RNA , Colombia , Transcriptome/genetics , Mosquito Vectors/genetics
16.
Rev. Soc. Bras. Med. Trop ; 54: e08912020, 2021. graf
Article in English | LILACS | ID: biblio-1250824

ABSTRACT

Abstract INTRODUCTION: Cerebrospinal fluid analysis contributes to the diagnosis and neuropathogenesis of neuroinvasive arboviruses. Neurological complications caused by dengue, Zika, and chikungunya infections have high clinical relevance because of their high potential to cause death or neurological deficits. We aimed to evaluate the use of cerebrospinal fluid assays for diagnostic support in neurological disorders associated with dengue, chikungunya, and Zika infections. METHODS: A systematic review was carried out by searching the electronic databases LILACS, PubMed, Scopus, and Embase for articles written in English, Portuguese, or Spanish in the last 19 years. Published studies were reviewed using the terms "dengue," "Zika", "chikungunya", alone or in combination with "cerebrospinal fluid" in the period from 2000 to 2019. RESULTS: A total of 98,060 studies were identified; of these, 1.1% (1,041 studies, 58,478 cases) used cerebrospinal fluid assays for neurological investigations. The most frequent neurological disorders included encephalitis (41.4%), congenital syndromes (17%), and microcephaly associated with Zika virus infections (8.9%). Neuroinvasive disorders were confirmed in 8.03% of 58,478 cases by specific cerebrospinal fluid analyses. The main methods used were IgM-specific antibodies (66%) and reverse transcription-polymerase chain reaction (10%). The largest number of scientific papers (29%) originated from Brazil, followed by India (18.4%) and the United States (14.4%). CONCLUSIONS: Although cerebrospinal fluid analysis is of great importance for increasing neurological diagnostic accuracy and contributes to the early diagnosis of neuroinvasive dengue, chikungunya, and Zika infections, it is underused in routine laboratory investigations worldwide.


Subject(s)
Humans , Chikungunya virus , Dengue/diagnosis , Dengue Virus , Chikungunya Fever/diagnosis , Zika Virus , Zika Virus Infection/diagnosis , Brazil
17.
Medicina (B.Aires) ; 80(supl.6): 94-96, dic. 2020.
Article in Spanish | LILACS | ID: biblio-1250324

ABSTRACT

Resumen El dengue es una arbovirosis confinada a las áreas geográficas donde habitan sus vectores, los mosquitos Aedes aegypti y Aedes albopictus. La transmisión ocurre principalmente durante el verano, pero la persistencia del insecto en el ambiente puede extenderla hasta el otoño en climas templados y cálidos. En nuestro país, este año la trasmisión estacional del dengue se superpuso temporalmente con la pandemia de COVID-19, producida por el SARS-CoV-2, un coronavirus causante de afecciones respiratorias graves con eventual desenlace fatal. Por otro lado, el HIV es un retrovirus que debilita el sistema inmune favoreciendo las infecciones por numerosos patógenos oportunistas. Presentamos el caso de un paciente con infección HIV sin tratamiento que desarrolló infección simultánea por dengue y SARS-CoV-2 con evolución favorable.


Abstract Dengue is a mosquito-borne viral disease confined to the geographical areas where its vectors Aedes aegypti and Aedes albopictus are present. The transmission occurs mainly during the summer but it can extend to autumn months in warm climates. In our country, this year the seasonal transmission of dengue overlapped with the COVID-19 pandemic, caused by SARS-CoV-2, a novel coronavirus responsible of serious respiratory conditions with eventual fatal outcomes. On the other hand, the HIV is a retrovirus that causes disease by weakening the immune system, favoring infections by numerous opportunistic pathogens. We present the case of an untreated HIV patient who developed simultaneous infection with dengue and SARS-CoV-2 with favorable evolution.


Subject(s)
Humans , Animals , Aedes , Dengue/complications , Dengue/diagnosis , Dengue Virus , Coinfection , COVID-19 , HIV Infections/complications , Dengue/epidemiology , Pandemics , Mosquito Vectors , SARS-CoV-2
18.
Rev. pediatr. electrón ; 17(1): 1-12, abr 2020.
Article in Spanish | LILACS | ID: biblio-1099829

ABSTRACT

Introducción: El dengue representa un importante problema de salud pública en países como Colombia. Usualmente se trata de una patología autolimitada con buena respuesta a manejo sintomático ambulatorio, sin embargo, cuadros con síntomas atípicos pueden complicar el diagnóstico dando lugar a manifestaciones inusuales que comprometen la vida del paciente. Objetivo: Describir la información del tema expuesto resaltando aspectos relevantes como diagnóstico y manejo. Metodología: Se efectuó una revisión bibliográfica con búsqueda electrónica en las siguientes bases de datos: PubMed, MEDLINE, Medscape, Scopus; en inglés y español incluyéndose diferentes tipos de artículo (artículos originales, revisiones de temas y guías de manejo que abordaran síntomas inusuales de dengue en pediatría desde el año 2009 al 2019. Resultados: Se obtuvo 414 referencias después de una revisión de artículos, donde se describe el tema de complicaciones relacionadas con esta patología en pediatría, se seleccionaron 50 artículos en texto completo que cumplían los requisitos de búsqueda. Conclusiones: El tema de manifestaciones atípicas del dengue tiene poca revisión registrada en el área de pediatría, son complicaciones poco frecuentes por lo cual la evidencia es escasa, estas se deben tener en cuenta para manejo y tratamiento oportuno.


Introduction: Dengue represents a major public health problem in countries like Colombia. Usually it is a self-limited pathology with good response to outpatient symptomatic management, however, pictures with atypical symptoms can complicate the diagnosis resulting in unusual manifestations that compromise the patient's life. Objective: Describe the information on the exposed topic highlighting relevant aspects such as diagnosis and management. Methodology: A bibliographic review was carried out with electronic search in the following databases: PubMed, MEDLINE, Medscape, Scopus; in English and Spanish including different types of article (original articles, review of topics and management guides that will address unusual symptoms of dengue in pediatrics from 2009 to 2019. Results: 414 references were obtained after an article review, which describes the topic of complications related to this pathology in pediatrics, 50 articles were selected in full text that met the search requirements. Conclusions: The issue of atypical manifestations of dengue has little revision registered in the area of pediatrics, they are rare complications, so the evidence is scarce, these should be taken into account for timely management and treatment.


Subject(s)
Humans , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Dengue/complications , Dengue/diagnosis , Dengue/pathology , Dengue Virus , Disease Progression , Acute Kidney Injury/etiology , Gastrointestinal Diseases/etiology , Nervous System Diseases/etiology
19.
Braz. j. infect. dis ; 24(1): 58-64, Feb. 2020. tab, graf
Article in English | LILACS | ID: biblio-1089331

ABSTRACT

ABSTRACT Background: Although performance of rapid immunochromatographic tests (RITs) for dengue virus (DENV) serotypes 1, 2 and 3 is relatively settled, evidence on accuracy of RITs for DENV-4 are based on studies with small sample sizes and with discrepant results. Objectives: To assess accuracy and inter-observer agreement of RITs targeting dengue nonstructural protein-1 (NS1) antigen - Dengue NS1-Bioeasy™, Dengue NS1 Ag Strip-Bio-Rad™, IVB Dengue Ag NS1-Orangelife™ and Dengue NS1-K130-Bioclin™ in DENV-4 samples. Methods: Study sample (n = 324) included adults presenting at an emergency unit in Rio de Janeiro, Brazil, with fever of ≤72 h and two or more dengue symptoms. A serum sample from each patient was tested by each RIT. A positive reverse-transcription polymerase chain reaction was considered as the reference standard for dengue diagnosis. The diagnostic parameters analyzed for each RIT were sensitivity, specificity, positive and negative predictive values, and likelihood ratios. Each RIT was read by homogeneous (two junior nurses) or heterogeneous (one junior nurse and one senior biologist) pairs. Agreement was estimated by simple kappa with 95% confidence interval, positive (Ppos) and negative (Pneg) proportion concordance and prevalence and bias adjusted kappa, rated from poor (k < 0.0) to almost perfect (0.8 < k < 1.0), and perfect (k = 1). Results: NS1 RITs for DENV-4 diagnosis showed high specificity (95.9%-99.4%), but low sensitivity (14.7%-45.4%). Bioeasy™ had the best performance, with a positive likelihood ratio of 26.0 (95% CI: 8.4;81.0). Inter-observer agreement was almost perfect for all evaluated RITs. Mismatches in confirmed dengue were more common for the Bioclin™ (Ppos 88.3-90.0 %) and Orangelife™ (Ppos 91.7-94.1 %) tests. Conclusions: For DENV-4, the tested RITs had high specificity, but lower sensitivity compared to published results for other serotypes. They should not be used for screening purposes. Different brands may have very different performances. This should be considered upon deciding of using RITs in DENV-4 outbreaks.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Chromatography, Affinity/standards , Dengue/diagnosis , Dengue Virus/isolation & purification , Reference Standards , Brazil , Enzyme-Linked Immunosorbent Assay , Observer Variation , Cross-Sectional Studies , Prospective Studies , Reproducibility of Results , Chromatography, Affinity/methods , Sensitivity and Specificity , Reverse Transcriptase Polymerase Chain Reaction , Dengue/immunology , Dengue/virology , Serogroup
20.
Braz. j. infect. dis ; 24(1): 85-88, Feb. 2020. tab, graf
Article in English | LILACS | ID: biblio-1089325

ABSTRACT

ABSTRACT The antigenic potential of seven immunogenic peptides of the dengue virus was evaluated in the sera of patients with dengue confirmed by IgM/IgG serology. Antibodies IgM and IgG against dengue virus peptides were analyzed by ELISA in 31 dengue sero-positive and 20 sero-negative patients. The P5 peptide showed significant IgG immunoreactivity mostly in the sera of patients with dengue without warning signs in comparison with patients with dengue with warning signs, correlating with mild disease. This finding suggests that the low antibody response against P5 epitope could be a risk factor for higher susceptibility to dengue virus infection with warning signs, and that P5 could be a potential antigen for vaccine development.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Young Adult , Peptides/immunology , Viral Envelope Proteins/immunology , Dengue Virus/immunology , Dengue Vaccines , Antibodies, Viral/immunology , Epitopes/immunology , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Enzyme-Linked Immunosorbent Assay , Statistics, Nonparametric , Dengue/immunology , Dengue/prevention & control , Antibody Formation , Antigens, Viral/immunology
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