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1.
Rev. Odontol. Araçatuba (Impr.) ; 42(1): 9-13, jan.-abr. 2021. tab
Article in Portuguese | LILACS, BBO | ID: biblio-1148159

ABSTRACT

O objetivo do presente estudo foi mensurar o pH externo radicular de dentes bovinos que foram desinfetados em solução de cloramina por 7 dias. Neste estudo foram utilizadas soluções irrigadoras, hipoclorito de sódio 1%, clorexidina 0,12% e hipoclorito de sódio 2,5% associadas ao edta e as medicações intracanais, hidróxido de cálcio, hidróxido de cálcio com PMCC e clorexidina gel 2%. O preparo químico mecânico foi realizado com as limas easy logic e as soluções foram agitadas com ultrassom durante 3 minutos e após os dentes foram imersos em água deionizada em eppendorfs estéreis e mantidos em estufa a 37°C. O pH externo foi analisado utilizando as fitas medidoras de pH no período de 24 horas, 48 horas, 7 dias, 10 dias e 15 dias. A normalidade dos valores obtidos de cada ensaio foi testada através do teste Kolmogorof- Smirnov, e o teste estatístico foi ANOVA de uma via e comparações múltiplas de Tukey. Os resultados mostraram que houve diferença estatística nos valores de pH nos grupos avaliados dentro dos tempos (p< 0,05). Concluiu-se que mesmo com as variações de pH nos períodos avaliados, as medicações e as soluções irrigantes podem ser utilizadas de forma associadas na endodontia, com o intuito de eliminar o maior número de microrganismos dos canais radiculares(AU)


The objective of the present study was to measure the external root pH of bovine teeth that were disinfected in chloramine solution for 7 days. Irrigating solutions, 1% sodium hypochlorite, 0.12% chlorhexidine and 2.5% sodium hypochlorite associated with edta and intracanal medications, calcium hydroxide, calcium hydroxide with PMCC and chlorhexidine gel 2%. The mechanical chemical preparation was performed with the easy logic files and the solutions were shaken with ultrasound for 3 minutes and after the teeth were immersed in deionized water in eppendorfs and kept in an oven at 37 ° C. The external pH was analyzed using the pH measuring tapes in the period of 24 hours, 48 hours, 7 days, 10 days and 15 days. The normal values obtained from each test were tested using the Kolmogorof-Smirnov test, and the statistical test was one-way ANOVA and Tukey's multiple comparisons. The results showed that there was a statistical difference in pH values in the groups evaluated within the times (p< 0.05). It was concluded that even with pH variations in the evaluated periods, medications and irrigating solutions can be used in an associated way in endodontics, in order to eliminate the largest number of microorganisms in the root canals(AU)


Subject(s)
Root Canal Irrigants , Root Canal Preparation , Sodium Hypochlorite , Calcium Hydroxide , Chloramines , Chlorhexidine , Analysis of Variance , Edetic Acid , Endodontics
3.
Braz. oral res. (Online) ; 35: e012, 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1132751

ABSTRACT

Abstract The purpose of this study was to analyze the effect of the application of different concentrations of EDTA on the adhesion of fiber posts to root dentin using self-adhesive resin cements. After endodontic treatment, 78 single roots were randomly divided into six groups (n = 13) according to the combination of the following factors: surface dentin treatment - control (distilled water), 17% EDTA and 24% EDTA; and self-adhesive resin cement - RelyX U200 (RX); and Multilink Speed (ML). After fiber post cementation, six slices were obtained for each root. Ten roots of each group were used for bond strength (BS) and three for microhardness (MH) evaluations. Data obtained from BS and MH tests for each resin cement were subjected to two-way ANOVA (surface treatment vs. root region) and to a post-hoc Tukey's test (α = 0.05). The lowest BS value was observed in the 24% EDTA group for RX cement, whereas the highest values for ML cement were observed for the control group in the middle and apical regions. In the MH test, the lowest value for RX was observed for 24% EDTA in the cervical region, whereas and the highest value for the ML cement was observed in the control group. Regarding both self-adhesive resin cements tested, the application of 24% EDTA was not able to improve the adhesion of fiber posts to root canal.


Subject(s)
Resin Cements , Materials Testing , Post and Core Technique , Edetic Acid/pharmacology , Dental Pulp Cavity , Dentin , Glass
4.
Int. j. odontostomatol. (Print) ; 14(4): 632-638, dic. 2020. tab, graf
Article in English | LILACS | ID: biblio-1134550

ABSTRACT

ABSTRACT: The aim of this in vitro study was to investigate the influence of ethylenediaminetetraacetic acid (EDTA) associated with the benzalkonium chloride (BAK) on the adhesion and formation of Enterococcus faecalis biofilms attached to coated dentin. Discs standard bovine dentin blocks were treated with the coating materials evaluated: Saline solution (control), 17 % EDTA, 17 % EDTA associated with 1 % BAK for 5 minutes and subsequently washed with saline solution. Afterwards, biofilms of E. faecalis (ATCC 29212) were grown on the surface of coated dentin blocks for time intervals of 1 hour and 7 days (n = 20) and were subsequently washed with phosphate-buffered saline (PBS). Bacterial viability and total biovolume were analyzed by confocal laser scanning microscopy (CLSM) using the Live/Dead technique. Nonparametric Kruskal-Wallis followed by Dunn tests were used to determine statistical differences (a = 5 %). The 17 % EDTA + 1 % BAK group showed significantly lower biovolume and bacterial viability values at the end of 1 hour (p < 0.05). After 7 days of contamination, the 17 % EDTA and 17 % EDTA + 1 % BAK groups showed similar results that differed statistically from those of the control group (p < 0.05). The saline solution group showed higher values. The use of BAK associated with EDTA on dentin blocks surfaces before exposure to contamination was able to interfere in the adhesion of E. faecalis to dentin. Also, dentin treatment by BAK associated with a chelating agent influences the secondary biofilm formation, which could have important effects on the long-term success of root canal treatment.


RESUMEN: El objetivo del estudio consistió en investigar in vitro, la influencia del ácido etilendiamino-tetraacético (EDTA) con cloruro de benzalconio (BAK) en la adhesión y formación de biopelículas de Enterococcus faecalis a la dentina. Discos de dentina bovina fueron tratadas con solución salina (control), 17 % de EDTA, 17% de EDTA asociado con 1 % de BAK durante 5 minutos y lavadas con solución salina. Las biopelículas de E. faecalis (ATCC 29212) se cultivaron sobre los discos de dentina durante intervalos de tiempo de 1 hora y 7 días (n = 20), lavados con solución salina tamponada con fosfato (PBS). La viabilidad bacteriana y el biovolumen total se analizaron mediante microscopía de barrido por láser (CLSM) utilizando la técnica Live / Dead. Se realizó prueba no paramétrica de Kruskal-Wallis, seguida por Dunn con una diferencia estadística (a = 5 %). El grupo de 17 % EDTA + 1 % BAK mostró valores significativamente menores de biovolumen y viabilidad bacteriana al final de 1 hora (p < 0,05). Después de 7 días de contaminación, los grupos de 17 % EDTA y 17 % EDTA + 1 % BAK mostraron resultados similares que diferían estadísticamente del grupo control (p < 0,05). La solución salina mostró valores más altos. La asociación de BAK con EDTA antes de la contaminación interfirió en la adhesión de E. faecalis. Además, el tratamiento de la dentina por BAK asociado con EDTA influye en la formación de biopelículas secundarias, lo que podría tener efectos importantes sobre el éxito a largo plazo del tratamiento del conducto radicular.


Subject(s)
Animals , Cattle , Bacterial Adhesion/drug effects , Edetic Acid/pharmacology , Enterococcus faecalis/growth & development , Enterococcus faecalis/drug effects , Biofilms/drug effects , Dentin/microbiology , Benzalkonium Compounds/pharmacology , Statistical Analysis , Microscopy, Confocal , Saline Solution
5.
Rev. Odontol. Araçatuba (Impr.) ; 41(3): 40-47, set./dez. 2020. ilus, graf
Article in Portuguese | LILACS, BBO | ID: biblio-1121745

ABSTRACT

Este trabalho consiste em uma pesquisa realizada sobre a utilização de líquidos irrigantes por cirurgiões dentistas na cidade de Itabuna-BA. Para isto, delimitou-se a seguinte problemática: quais são as escolhas dos líquidos irrigantes nos tratamentos endodônticos com o intuito de promover uma total desinfecção dos canais radiculares? E como objetivo geral, elucidar sobre as escolhas dos cirurgiões dentistas localizados na cidade de Itabuna-Bahia em relação as substâncias utilizadas como coadjuvantes no tratamento endodôntico durante a etapa de irrigação. A pesquisa teve uma abordagem qualitativa e foi utilizado o instrumento questionário com cem dentistas da cidade selecionada. Este questionário consistia em oito perguntas, dentre elas qual irrigante utilizado e a porcentagem do mesmo. Com relação aos resultados, pode-se afirmar que no tratamento endodôntico, o hipoclorito de sódio continua sendo a solução irrigante de escolha entre os profissionais, diferentes concentrações de soluções de hipoclorito de sódio são empregadas durante o preparo biomecânico, por endodontistas e clínicas gerais que praticam a endodontia, não existindo uma unanimidade quanto a escolha, nenhum acordo existe em relação a sua concentração ideal(AU)


This work consists of a research carried out on the use of irrigating liquids by dental surgeons in the city of Itabuna-BA. For this, the following problem was defined: what are the choices of irrigating liquids in endodontic treatments in order to promote a total disinfection of the root canals? And as a general objective, elucidate about the choices of dentists located in the city of Itabuna-Bahia in relation to the substances used as adjuvants in endodontic treatment during the irrigation stage. The research had a qualitative approach and the questionnaire was used with 100 dentists in the selected city. This questionnaire consisted of eight questions, including which irrigant was used and the percentage of it. Regarding the results, it can be said that in endodontic treatment, sodium hypochlorite remains the irrigating solution of choice among professionals, different concentrations of sodium hypochlorite solutions are used during biomechanical preparation by endodontists and general practitioners who practice endodontics, there is no unanimity as to the choice, no agreement exists regarding their ideal concentration(AU)


Subject(s)
Root Canal Irrigants , Root Canal Preparation , Sodium Hypochlorite , Chlorhexidine , Edetic Acid , Malicum Acidum
6.
J. oral res. (Impresa) ; 9(6): 490-499, dic. 31, 2020. ilus, tab
Article in English | LILACS | ID: biblio-1178945

ABSTRACT

Introduction: This study aimed to evaluate the effect of dentin surface treatment with 37% phosphoric acid or 17% ethylenedia-minetetraacetic acid (EDTA) before Internal Bleaching with 35% hydrogen peroxide using the walking bleach technique. Material and Methods: This experimental in vitro study used 66 human premolars extracted for orthodontic reasons, which were debrided, endodontically prepared, and pigmented with chromogens derived from blood decomposition. The samples were randomly divided into three groups (n=22). Group A: bleaching agent without dentin conditioning; group B: bleaching agent in dentin conditioned with phosphoric acid 37%; group C: bleaching agent in dentin conditioned with 17% EDTA. 4 applications of bleaching agent were used with a separation of 4 days between each session. The initial color (baseline) and after each application was determined by spectrophotometry, recording the CIE L*a*b* values and the total color variation between the initial parameters and the different evaluation times. Results: Data were statistically analyzed with the Wilcoxon test. This showed statistically significant differences for the total variation of the color between the study groups, with the control group in no case inferior to the rest. Conclusion: The application of 37% phosphoric acid increased the effectiveness of the bleaching agent when compared to 17% EDTA. However, these did not increase the effectiveness compared to the application of the bleaching agent without a previous dentin surface treatment.


Introducción: El objetivo de este estudio fue evaluar el efecto del tratamiento de superficie dentinaria con ácido fosfórico al 37% o EDTA al 17% previo al blanqueamiento Interno con peróxido de hidrógeno al 35% por medio de la técnica Walking Bleach. Material y Métodos:Para este estudio experimental in vitro, se utilizaron 66 premolares humanos extraídos por indicación ortodóncica, los cuales fueron desbridados, preparados endodónticamente, y pigmentados con cromógenos derivados de la descomposición sanguínea. Las muestras fueron divididas aleatoriamente en 3 grupos (n=22). Grupo A: agente blanqueador sin acondicionamiento dentinario, grupo B: agente blanqueador en dentina acondicionada con ácido fosfórico 37% y grupo C: agente blanqueador en dentina acondicionada con EDTA 17%. Se utilizaron 4 aplicaciones de agente blanqueador con una separación de 4 días entre cada sesión. El color inicial (baseline) y tras cada aplicación fue determinado mediante espectrofotometría, registrando los valores CIE L*a*b* y la variación total de color entre los parámetros iniciales y los diferentes tiempos de evaluación. Resultados: Los datos fueron analizados estadísticamente con la prueba de Wilcoxon, arrojando diferencias estadísticamente significativas para la variación total del color entre los grupos de estudio, siendo en ningún caso el grupo control inferior al resto. Conclusión: La aplicación de ácido fosfórico al 37% aumenta la eficacia del agente blanqueador al compararlo con el EDTA 17%, sin embargo, no aumentan la eficacia respecto a la aplicación del agente blanqueador sin un tratamiento de superficie dentinaria previo.


Subject(s)
Humans , Edetic Acid/chemistry , Color , Dental Enamel/chemistry , Dentin/chemistry , Hydrogen Peroxide/chemistry , Spectrophotometry , Bicuspid , In Vitro Techniques , Chile , Epidemiology, Experimental
7.
Arq. bras. med. vet. zootec. (Online) ; 72(6): 2119-2126, Nov.-Dec. 2020. tab
Article in English | LILACS, VETINDEX | ID: biblio-1142317

ABSTRACT

We evaluated the effect of reducing free calcium in the cryopreservation medium, using the calcium chelator ethylene diamine tetracetic acid (EDTA) at 0.3% and 0.5% concentrations. Three male mixed breed dogs were subjected to semen collection by digital manipulation (n=16). Each ejaculate was divided in three aliquots, and each one was diluted in TRIS-glucose-egg yolk extender with 6% glycerol and 0.5% Equex STM Paste® (TGE, control); and added with 0.3% EDTA (EDTA 0.3) or 0.5% EDTA (EDTA 0.5). Calcium concentration reduced in EDTA 0.3 and all the calcium ions were chelated in EDTA 0.5. The EDTA addition did not affect sperm morphology or plasma membrane integrity; however, by removing all free calcium (EDTA 0.5), the sperm motility reduced (64.7% in TGE and 45% in EDTA 0.5; p<0.05). Acrosome integrity and sperm binding ability were not improved by calcium chelation. The failure to prevent the premature AR may explain why sperm longevity was not affected by calcium removal. Thus, the partial or complete calcium removal, through EDTA addition, is not able to prevent acrosomal damage or premature acrosomal reaction, and therefore does not improve the dog sperm binding ability.(AU)


Avaliou-se o efeito da redução do cálcio livre no meio de congelamento, usando-se o quelante de cálcio etilenodiaminotetracético (EDTA) a 0,3% e 0,5%. Três cães machos sem raça definida foram submetidos à coleta de sêmen por manipulação digital (n=16). Cada ejaculado foi diluído em diluidor controle com TRIS-glicose - gema de ovo (TGE, controle), ou em diluidor TGE enriquecido com 0,3% (EDTA 0,3) ou 0,5% de EDTA (EDTA 0,5). A concentração de cálcio reduziu no meio EDTA 0,3, e todos os íons de cálcio foram quelados no meio EDTA 0,5. A adição do EDTA e a consequente quelação do cálcio não afetaram a morfologia espermática ou a integridade da membrana plasmática, no entanto, ao remover todo o cálcio do meio (EDTA 0,5), a motilidade espermática se reduziu (64,7% no TGE e 45% no EDTA 0,5; P<0,05). A integridade do acrossoma e a capacidade de ligação do espermatozoide não melhoraram com a quelação do cálcio. Apesar da influência da concentração de cálcio sobre a motilidade espermática após o descongelamento, a falha em prever a reação acrossomal prematura pode explicar por que a longevidade espermática não foi afetada pela remoção do cálcio no meio. Dessa forma, a remoção parcial ou total do cálcio, por meio da adição de EDTA, não é capaz de prevenir o dano no acrossoma ou a reação acrossomal prematura e, portanto, não aumenta a capacidade do espermatozoide de se ligar ao oócito.(AU)


Subject(s)
Animals , Male , Dogs , Semen Preservation/veterinary , Sperm Agglutination , Edetic Acid/analysis , Acrosome Reaction , Calcium Chelating Agents/analysis , Cryopreservation/veterinary
8.
Rev. Odontol. Araçatuba (Impr.) ; 41(2): 28-33, maio-ago.2020. tab, graf
Article in Portuguese | LILACS, BBO | ID: biblio-1102669

ABSTRACT

O objetivo do presente estudo foi avaliar o pH externo radicular de dentes bovinos pré-selecionados. Neste estudo foram utilizadas soluções irrigadoras, ácido peracético 0,25%, hipoclorito de sódio 2,5% e hipoclorito de sódio 6% associadas ao EDTA e as medicações intracanais, ultracal, hidróxido de cálcio P.A. associado à clorexidina gel 2% e hidróxido de cálcio P.A. associado ao propilenoglicol. O Preparo químico mecânico foi realizado com as limas easy logic e as soluções agitadas com easy clean durante 3 minutos, após os dentes foram imersos em água deionizada em eppendorfs estéreis e mantidos em estufa a 37°C. O pH externo foi mensurado utilizando fitas de pH no período de 3, 24, 72 horas, 7 e 14 dias. A normalidade dos valores obtidos de cada ensaio foi testada através do teste Kolmogorof- Smirnov e o teste estatístico foi ANOVA de uma via e comparações múltiplas de Tukey. Os resultados mostraram que os grupos apresentaram diferença estatística entre eles, entretanto apresentaram alcalinidade durante o período avaliado de 24, 48,72 horas, 7 e 14 dias. Concluiu-se que as soluções associadas às diferentes medicações mostraram pH alcalino, apresentando diferença entre os grupos avaliados nos tempos de 3, 24, 72 horas, 7 e 14 dias, no entanto mostraram resultados satisfatórios, podendo ser utilizados na endodontia(AU)


The objective of the present study was to evaluate the external root pH of pre-selected bovine teeth. In these study irrigation solutions, 0.25% peracetic acid, 2.5% sodium hypochlorite, sodium hypochlorite 6% associated with EDTA and intracanal medications, ultracal, calcium hydroxide PA associated with chlorhexidine gel 2% and P.A. calcium hydroxide associated with propyleneglycol. The mechanical chemical preparation was performed with the easylogic files and the solutions stirred with easy clean for 3 minutes, after the teeth were immersed in deionized water in sterile eppendorfs and kept in an oven at 37 °C. The external pH was measured using pH tapes in the period of 3, 24, 72 hours, 7 and 14 days. The normal values obtained from each test were tested using the KolmogorofSmirnov test and the statistical test chosen was oneway ANOVA and Tukey's multiple comparisons. The results showed that the groups presented statistical difference between them, however they presented alkalinity during the evaluated period of 24, 48, 72 hours, 7 and 14 days. It was concluded that the solutions associated with the different medications showed alkaline pH, presenting a difference between the groups evaluated at 3, 24, 72 hours, 7 and 14 days, however they showed satisfactory results and could be used in endodontics(AU)


Subject(s)
Root Canal Filling Materials , Root Canal Preparation , Peracetic Acid , Root Canal Therapy , Sodium Hypochlorite , Calcium Hydroxide , Chlorhexidine , Edetic Acid , Propylene Glycol
9.
Revista Naval de Odontologia ; 47(1): [7-13], 12/06/2020.
Article in English | LILACS | ID: biblio-1359694

ABSTRACT

This study evaluated the ex vivo effectiveness of different final irrigation protocols in smear layer removal and intraradicular dentine erosion. Thirty five extracted human canines were instrumented and randomly divided, according to final rinse techniques used, into 7 groups: 1 (ED3M) and 3 (ED3US), 17% EDTA for 3 minutes with manual and ultrasonic agitation, respectively; 2 (CA30M) and 4 (CA30US), 10% citric acid for 30 seconds with manual and ultrasonic agitation, respectively; 5 (CA3M) and 6 (CA3US), 10% citric acid for 3 minutes with manual and ultrasonic agitation, respectively; and, 7 (Na3), 5.25% NaOCl for 3 minutes without agitation (control). All specimens then were irrigated with 5.25% NaOCl, split lengthwise, and examined under scanning electron microscopy (SEM) in apical, middle and coronal thirds. Data were analyzed with Kruskal-Wallis and Mann-Whitney tests. The effectiveness of 17% EDTA and 10% citric acid in removing smear layer was significantly greater than 5.25% NaOCl (control). There were no significant differences among final irrigation protocols in smear layer removal or erosive effects. However, when comparing the thirds in groups 1 (ED3M) and 2 (CA30M), the least smear layer removal and erosion was seen in the apical third, but the group 4 procedure (CA30US) was more effective than 1 (ED3M) (p=0.0004), 2 (CA30M) (p=0.0018) or 3 (ED3US) (p=0.0003) in smear layer removal for the apical third. It was concluded that protocols used in this study were similar in smear layer removal and erosive effects.


Subject(s)
Humans , Male , Female , Tooth Erosion , Microscopy, Electron, Scanning , Edetic Acid , Citric Acid
10.
Braz. j. otorhinolaryngol. (Impr.) ; 86(2): 222-227, March-Apr. 2020. graf
Article in English | LILACS | ID: biblio-1132576

ABSTRACT

Abstract Introduction: The use of electron microscopy in the study of the inner ear has allowed us to observe minute details of the hair cells, especially in ototoxicity studies; however, the preparation of this material is a difficult and delicate task. In an attempt to simplify the handling of these materials, two agents, toluidine blue and ethylenediamine tetra-acetic acid were tested, in addition to the elimination of osmium tetroxide during the preparation of albino guinea pig cochleae. We also tested the applicability of these methodologies in an ototoxicity protocol. Objective: To verify the quality of the images obtained with and without the use of ethylenediamine tetra-acetic acid, toluidine blue and osmium tetroxide in the preparation of cochleae of albino guinea pigs for the scanning electron microscopy. Methods: Three groups of cochleae were used. In Group 1, 10 cochleae were prepared with the usual methodology, dissecting the optical capsule without decalcification and using osmium tetroxide as a post-fixative agent. In Group 2, we prepared 10 cochleae decalcified with ethylenediamine tetra-acetic acid, injecting toluidine blue in the endolymphatic space to facilitate the identification of the organ of Corti. In Group 3, we used 4 cochleae of guinea pigs that received 3 doses of cisplatin (7.5 mg/kg, D1-D5-D6), two prepared according to the methodology used in Group 1 and two with that used in Group 2. Scanning electron microscopy images were obtained from the organ of Corti region of the basal turn of each cochlea. Results: The organ of Corti was more easily identified with the use of toluidine blue. The dissection of the cochlea was more accurate in the decalcified cochleae. The quality of the images and the preservation of the organ of Corti obtained with the two methodologies were similar. Conclusion: The proposed modifications resulted in images of similar quality as those observed using the traditional methodology.


Resumo Introdução: O emprego da microscopia eletrônica no estudo da orelha interna permitiu observar detalhes minuciosos das células ciliadas especialmente em estudos de ototoxicidade. Entretanto, o preparo desse material é trabalhoso e delicado. Para simplificar a manipulação desses materiais, testou-se o uso de dois agentes, azul de toluidina e ácido etilenodiamino tetra-acético, além da retirada do tetróxido de ósmio na preparação de cócleas de cobaias albinas. Testamos também a aplicabilidade dessas metodologias em um protocolo de ototoxicidade. Objetivo: Verificar a qualidade das imagens obtidas com e sem o uso de ácido etilenodiamino tetra-acético, azul de toluidina e tetróxido de ósmio na preparação de cócleas de cobaias albinas para a microscopia eletrônica de varredura. Método: Foram utilizados três grupos de cócleas. No Grupo 1 preparou-se 10 cócleas com a metodologia usual, dissecando a cápsula ótica sem descalcificac¸ão e utilizando tetróxido de ósmio como pós-fixador. No Grupo 2 preparamos 10 cócleas descalcificadas com ácido etilenodiamino tetra-acético, injetando azul de toluidina no espac¸o endolinfático para facilitar a identificação do órgão de Corti. No Grupo 3 utilizamos 4 cócleas de cobaias que receberam 3 doses de cisplatina (7,5 mg/kg, D1-D5-D6), duas preparadas com a metodologia do Grupo 1 e duas com a do Grupo 2. Foram obtidas imagens da microscopia eletrônica de varredura da região do órgão de Corti do giro basal de cada cóclea. Resultados: O órgão de Corti foi mais facilmente identificado com o azul de touidina. A dissecção da cóclea foi mais precisa nas cócleas descalcificadas A qualidade das imagens e a preservac¸ão do órgão de Corti obtidas com as duas metodologias foi similar. Conclusão: As modificações propostas resultaram em imagens de qualidade similar as observadas com o uso da metodologia tradicional.


Subject(s)
Animals , Female , Cisplatin/toxicity , Cochlea/drug effects , Cochlea/ultrastructure , Organ of Corti/drug effects , Organ of Corti/ultrastructure , Osmium Tetroxide/administration & dosage , Tolonium Chloride/administration & dosage , Microscopy, Electron, Scanning , Edetic Acid/administration & dosage , Guinea Pigs , Hair Cells, Auditory/drug effects , Hair Cells, Auditory/ultrastructure
11.
J. appl. oral sci ; 28: e20190005, 2020. tab, graf
Article in English | LILACS | ID: biblio-1056579

ABSTRACT

Abstract Chitosan is a natural, biocompatible chelating substance with potential for dental use. This study compared the effects of final canal irrigation with chitosan and EDTA on dentin microhardness, sealer dentin tubules penetration capacity, and push-out strength. Methodology: Fifty canine roots were distributed according to the final irrigation protocol (n=10): G1- 15% EDTA with conventional irrigation; G2- 15% EDTA with Endovac; G3- 0.2% chitosan with conventional irrigation; G4- 0.2% chitosan with Endovac; and G5- without irrigation. Specimens were obturated (AH Plus) and sectioned in 3 slices per root third. The first slice was used for microhardness and sealer penetration assessments under a laser confocal microscope. The second was utilized in a push-out strength test. The third slice was discarded. Data were analyzed using 2-way ANOVA and Tukey's post hoc test (α<0.05). Failure mode was determined at x40 magnification. Results: Microhardness reduction was more significant in groups G2 and G4 (p<0.05). Sealer penetration through dentin was significantly greater in group G2 (p<0.05). There was no significant difference between groups G1, G3, and G4 (p>0.05). In general, all experimental groups presented similar bond resistance (p>0.05) that significantly differed from the control (p<0.001). Mixed type failures were predominant. Conclusions: In general, 0.2% chitosan and 15% EDTA solutions act in a similar manner with regard to the variables studied. The use of Endovac potentiates the effect of these solutions.


Subject(s)
Humans , Root Canal Irrigants/chemistry , Chelating Agents/chemistry , Dental Bonding/methods , Edetic Acid/chemistry , Dentin/drug effects , Chitosan/chemistry , Reference Values , Root Canal Filling Materials/chemistry , Surface Properties/drug effects , Materials Testing , Reproducibility of Results , Analysis of Variance , Microscopy, Confocal , Shear Strength , Epoxy Resins/chemistry , Hardness Tests , Middle Aged
12.
Med. lab ; 24(2): 131-140, 2020.
Article in Spanish | LILACS, COLNAL | ID: biblio-1097088

ABSTRACT

El EDTA es el anticoagulante de elección en los laboratorios de hematología para la conservación de la muestra de sangre total. Existen dos tipos, EDTA K2 y EDTA K3, y su diferencia radica en la cantidad de moléculas de potasio. Algunas guías sugieren que hay diferencias entre el anticoagulante EDTA K2 y el K3 para el proceso del hemograma; sin embargo, con las nuevas presentaciones de los tubos que traen las casas comerciales, no se tiene claro si en realidad aún hay diferencia entre los dos anticoagulantes, y si esto puede alterar el resultado del hemograma, tanto en el resultado cuantitativo, como en el cualitativo. Objetivo. Comparar los recuentos leucocitarios, la hemoglobina, el hematocrito, el volumen corpuscular medio, las plaquetas y la morfología celular en muestras de sangre periférica con EDTA K2 y EDTA K3, en diferentes tiempos (0, 1 y 2 horas). Materiales y métodos. Se realizó un estudio cuasi-experimental, multivariado, multifactorial, que tiene como unidad de análisis la sangre anticoagulada con EDTA K2 y EDTA K3, extraída de 53 individuos a través de un muestreo no probabilístico por conveniencia. Resultados. Al comparar los resultados del estudio morfológico por medio del extendido de sangre periférica y los datos cuantitativos del hemograma, se encontró que no hay diferencias estadísticamente significativas usando EDTA K2 o K3. Conclusión. Se evidenció que el uso del EDTA K2 o EDTA K3 como anticoagulante de elección, procesando las muestras en un tiempo adecuado después de su recolección, no afecta los parámetros cuantitativos del hemograma automatizado ni los morfológicos.


EDTA is the anticoagulant of choice in hematology laboratories for the conservation of whole blood samples. There are two types, K2 EDTA and K3 EDTA, and their difference lies in the amount of potassium molecules. Some guidelines suggest that there are differences between K2 and K3 EDTA for the blood analysis process. However, with the new collection tubes offered by the commercial suppliers, it is not clear if in fact there is a difference between the two anticoagulants that would result in changes in blood parameters and cell morphology. Objective. To compare leukocyte counts, hemoglobin, hematocrit, mean corpuscular volume, platelets and cell morphology in peripheral blood samples collected with K2 EDTA and K3 EDTA, at different times (0, 1 and 2 hours). Materials and methods. A quasi-experimental, multivariate, multifactorial study was carried out, with anticoagulated blood as the unit of analysis, either with K2 EDTA or K3 EDTA, extracted from 53 subjects through a non-probabilistic sampling for convenience. Results. There was no statistically significant difference when comparing results of the peripheral blood smear and the quantitative hematological parameters using K2 or K3 EDTA. Conclusion. The use of either K2 EDTA or K3 EDTA as the anticoagulant of choice, when processing samples within a suitable time after their collection, proved equally satisfactory for both quantitative and morphological parameters


Subject(s)
Humans , Blood Cells , Blood Cell Count , Edetic Acid
13.
Article in English | WPRIM | ID: wpr-880845

ABSTRACT

The aim of this study was to compare the efficiency of four final irrigation protocols in smear layer removal and bacterial inhibition in root canal systems. Thirty roots inoculated with Enterococcus faecalis were prepared with ProTaper Universal files. The teeth were disinfected by conventional needle irrigation, sonic agitation using the EndoActivator device, passive ultrasonic irrigation, or an M3 Max file. Teeth with no root canal preparation served as blank controls for the establishment of the infection baseline. Teeth with preparation but no final irrigation served as a post-instrumentation baseline. After the final irrigation, the teeth were sectioned in half. One half of each tooth was examined by scanning electron microscopy (SEM) to assess smear layer removal using a five-point scale. The other half was examined by confocal laser scanning microscopy (CLSM) using the LIVE/DEAD BackLight bacterial viability kit to evaluate the depth of bacterial survival in dentinal tubules. SEM analysis revealed no significant difference in smear layer removal throughout the whole canal among the EA, PUI, and M3 Max groups (P > 0.05). CLSM revealed that PUI achieved the greatest bacterial inhibition depth in the coronal ((174.27 ± 31.63) μm), middle ((160.94 ± 37.77) μm), and apical ((119.53 ± 28.49) μm) thirds of the canal (all P < 0.05 vs. other groups). According to this comprehensive SEM and CLSM evaluation, PUI appears to have the best infection control ability in root canal systems.


Subject(s)
Dental Pulp Cavity , Edetic Acid , Humans , Microscopy, Electron, Scanning , Root Canal Irrigants , Root Canal Preparation , Smear Layer , Sodium Hypochlorite
14.
Odontoestomatol ; 21(34): 5-15, Jul-Dic. 2019.
Article in Spanish | LILACS, BNUY | ID: biblio-1024952

ABSTRACT

The aim of this study was to find a formula and application time for chelators effective in cleaning the root canal without causing erosion and considering dental age. The sample included 120 teeth: 60 taken from young patients and 60 from adult patients. They were instrumented and irrigated with 2.5% sodium hypochlorite (NaOCl). Each subgroup was divided randomly into six groups: 4 groups of 12 teeth and 2 control groups of 6 teeth. In the final irrigation, 17% ethylenediaminetetraacetic acid plus Cetavlón (EDTAC) or 10% citric acid was applied for 1 or 3 minutes according to each group. The results showed a high level of cleanliness, and a few showed erosion of the dentinal tubules. Satisfactory results were obtained in the removal of the smear layer when applying EDTAC or citric acid combined with NaOCl. Erosion was present in different degrees regardless of dental age, but it was indeed affected by the irrigant exposure time.


O objetivo deste estudo foi obter uma fórmula e um tempo de aplicação dos quelantes efetivos que permitam a limpeza das paredes do canal sem causar erosões, levando em consideração a idade dental. A amostra foi composta por 120 dentes, 60 de pacientes jovens e 60 de pacientes adultos, foram instrumentados e irrigados com hipoclorito de sódio (NaOCl) a 2,5%. Cada subgrupo foi dividido aleatoriamente em 6 grupos: 4 grupos de 12 dentes e 2 grupos de controle com 6 dentes cada um. Na irrigação final, ácido etilenodiaminotetracético com Cetavlón (EDTAC) 17% ou ácido cítrico 10% foi aplicado por 1 ou 3 minutos, dependendo do grupo. Os resultados mostraram um bom nível de limpeza com algumas amostras com erosão dos túbulos dentinários. Com EDTAC ou ácido cítrico combinado com NaOCl, resultados satisfatórios foram obtidos na remoção da camada de esfregaço . A erosão esteve presente em seus diferentes graus independentemente da idade dentária, mas não do tempo de exposição do irrigante.


Subject(s)
Humans , Tooth Erosion , Edetic Acid , Ethylenediamines , Chelating Agents , Smear Layer
15.
Rev. ADM ; 76(5): 278-281, sept.-oct. 2019.
Article in Spanish | LILACS | ID: biblio-1053117

ABSTRACT

La instrumentación mecánica durante el tratamiento periodontal trae consigo la formación de escombros microcristalinos que inhiben la adhesión tisular a la superfi cie radicular y favorece la proliferación bacteriana, lo cual perjudica los resultados del tratamiento periodontal a corto y largo plazo. Hoy en día el acondicionamiento radicular con el uso de biomodifi cadores es una opción de tratamiento adicional en el tratamiento de la periodontitis y el tratamiento de cobertura radicular. El objetivo del presente estudio es realizar una revisión de la literatura acerca de las aplicaciones y del acondicionamiento radicular con ácido cítrico, tetraciclina, EDTA y láser en el tratamiento periodontal no quirúrgico y quirúrgico (AU)


Mechanical instrumentation during periodontal treatment brings the formation of microcrystalline debris that inhibits tissue adhesion to the root surface and favors bacterial proliferation, which harms the results of the short and long term periodontal treatment. Nowadays, root conditioning with the use of biomodifi cators is an additional treatment option in the treatment of periodontitis and root coverage therapy. The aim of the present study is to conduct a literature review about the applications and the root conditioning with citric acid, tetracycline, EDTA and laser in the non surgical and surgical periodontal treatment (AU)


Subject(s)
Humans , Periodontal Diseases/surgery , Periodontal Diseases/therapy , Tooth Root/drug effects , Tetracyclines , Dental Scaling , Root Planing , Edetic Acid , Citric Acid , Laser Therapy
16.
Rev. ADM ; 76(4): 214-218, jul.-ago 2019. ilus
Article in Spanish | LILACS | ID: biblio-1023643

ABSTRACT

Introducción: El uso de agentes químicos en la superficie radicular durante la fase higiénica periodontal tiene como ventajas eliminar factores irritantes, prevenir la acumulación bacteriana y fomentar la cicatrización de la herida. El objetivo del presente estudio fue evaluar la superficie radicular de órganos dentales con periodontitis crónica avanzada, tratados mediante terapia periodontal no quirúrgica con EDTA y láser CO2. Material y métodos: Se evaluaron 40 órganos dentarios de pacientes con periodontitis crónica avanzada indicados para extracción, a los cuales se les realizó raspado y alisado radicular, con el uso de EDTA al 24%, láser CO2 a energía de 1, 1.5 y 2 Watt, para posteriormente evaluar la superficie radicular mediante un microscopio electrónico de barrido. Resultados: El tratamiento de raspado y alisado radicular mostró una superficie regular, pero con marcas de estrías con una cubierta de escombro dentinario, las muestras tratadas con EDTA al 24% muestran apertura de los túbulos dentinarios, con bordes definidos y un diámetro aparentemente amplio y los órganos dentarios tratados con láser CO2 con diferentes poderes de energía mostraron múltiples cambios en su superficie, con una apertura de túbulos dentinarios en su mayoría. Conclusión: La apertura de los túbulos dentinarios se presentó en los grupos experimentales tratados con biomodificador radicular (AU)


Introduction: The use of chemical agents in the root surface during the periodontal hygienic phase has the advantages of eliminating irritating factors, preventing bacterial accumulation and promoting wound healing. The objective of the present study was to evaluate the radicular surface of dental organs with advanced chronic periodontitis treated by non-surgical periodontal therapy with EDTA and CO2 laser. Material and methods: Forty dental organs of patients with advanced chronic periodontitis indicated for extraction were evaluated, which were performed scraping and root planing, with the use of 24% EDTA, CO2 laser with energy of 1, 1.5 and 2 Watt, to later evaluate the radicular surface by means of a scanning electron microscope. Results: The treatment of scaling and root planing showed a regular surface, but with marks of striae with a covering of dentine rubble, the samples treated with 24% EDTA show opening of the dentinal tubules, with defined edges and a seemingly wide diameter and, the dental organs treated with CO2 laser with different power powers showed multiple changes in their surface, with an opening of dentinal tubules in their majority. Conclusion: The opening of the dentinal tubules was presented in the experimental groups treated with a root biomodifier (AU)


Subject(s)
Humans , Periodontitis/therapy , Tooth Root/drug effects , Dental Scaling , Edetic Acid , In Vitro Techniques , Microscopy, Electron, Scanning , Epidemiology, Descriptive , Cross-Sectional Studies , Prospective Studies , Lasers, Gas
17.
J. oral res. (Impresa) ; 8(3): 228-235, jul. 31, 2019. tab
Article in English | LILACS | ID: biblio-1145340

ABSTRACT

Objective. To evaluate the degree of marginal sealing and adhesive strength in Bulk-Fill resin by applying 18% EDTA gel compared to 37% phosphoric acid gel in dental conditioning. Materials and methods. The study was transversal, experimental comparative, and applied. The sample group consisted of 60 teeth with Class I cavities, which were later subjected to conditioning. This was carried out separately with phosphoric acid gel for 15 seconds, and 18% EDTA gel within two different time frames: 60 and 90 seconds. All samples were incubated at 37ºC for a period of 24 hours and thermocycled between 5 to 55ºC for 500 cycles. To assess microfiltration, they were immersed in methylene blue at 37ºC for 4 hours, and were later sectioned and observed through a stereomicroscope. In order to perform the traction, the conventional testing machine was used at a speed of 0.75mm/min until the screw with the resin detached. Results: Samples conditioned with 37% phosphoric acid showed a greater degree 1 microfiltration and a mean adhesive resistance of 7.97 MPa. The same number of microfiltration grades 1 and 2 was found when using EDTA gel for 60 seconds, with a mean tensile strength of 8.8 MPa. On the other hand, applying EDTA gel for 90 seconds showed mostly grade 1 microfiltrations, and to a lesser extent, grade 0 and 2, with a mean resistance of 9.2 MPa. Conclusion: There are no statistically significant differences regarding adhesive strength between the two gels; however, better marginal filling was observed when samples were conditioned with 18% EDTA gel for 90 seconds.


Objetivo. Evaluar el grado de sellado marginal y resistencia adhesiva en resina Bulk ­ Fill aplicando gel de EDTA al 18% en comparación al gel de ácido fosfórico al 37% en el acondicionamiento dental. Materiales y métodos. El tipo de estudio fue transversal, comparativo, de nivel aplicativo y diseño experimental. La muestra estuvo conformada por 60 dientes con cavidades clase I; se realizó el acondicionamiento con gel de ácido fosfórico, gel del EDTA al 18% en 60 segundos y 90 segundos. El total de las muestras fueron incubados a 37°C por 24 horas, se termocicló con 500 ciclos entre 5 a 55ºC. Para evaluar microfiltración, fueron sumergidos en azul de metileno a 37ºC por 4 horas, fueron seccionadas y observados al estereomicroscopio, para realizar la tracción se empleó la máquina de ensayos universales a una velocidad de 0.75mm/min hasta que se desprendiera el tornillo con la resina. Resultados. Las muestras acondicionadas con ácido fosfórico al 37% presenta mayor microfiltración grado 1 y el promedio de su resistencia adhesiva es de 7.97Mpa, la microfiltración obtenida con EDTA en 60 segundos presenta equidad entre grado 1 y 2; promedio de resistencia a la tracción de 8.8 Mpa y el EDTA 90 segundos presentó mayor microfiltración grado 1 y el resto 0 y 2; el promedio de resistencia fue 9.2 Mpa. Conclusión. No existen diferencias estadísticamente significativas al comparar los acondicionantes para la prueba de resistencia adhesiva, sin embargo se observó mejor sellado marginal cuando se acondicionó con gel de EDTA al 18 % por 90 segundos.


Subject(s)
Humans , Dental Bonding , Dental Marginal Adaptation , Composite Resins/chemistry , Dental Restoration, Permanent/methods , Temperature , Acid Etching, Dental , Microstraining , Double-Blind Method , Cross-Sectional Studies , Edetic Acid , Dental Materials
18.
Braz. dent. j ; 30(3): 220-226, May-June 2019. tab, graf
Article in English | LILACS | ID: biblio-1011555

ABSTRACT

Abstract The present study aimed to compare the effectiveness of QMiX and 17% EDTA associated to passive ultrasonic irrigation (PUI) or manual agitation (MA) on the reduction of E. faecalis, E. coli and LPS from root canals. Forty single rooted human teeth were randomly divided into four groups (n=10), according to the final irrigation protocol: EDTA+MA, QMiX+MA, EDTA+PUI, QMiX+PUI. Sample collections were obtained from the root canal content immediately before preparation (baseline-S1), after instrumentation (S2), after final irrigation protocol (S3) and 7 days after instrumentation and final irrigation (S4). The antimicrobial effectivity and on endotoxin content were analyzed by culture procedure (CFU/mL) and LAL assay (EU/mL), respectively. The results were statistically analyzed by Kruskal-Wallis and Friedman test (α=5%). QMiX+MA and QMiX+PUI reduced 100% of E. coli and E. faecalis bacteria and also prevented E. faecalisregrowth at S4. EDTA significantly reduced E. coli, but it was not effective in reducing E. faecalis. All protocols reduced EU/mL when compared to S1, however at S4 there was a significant reduction of EU/mL only in the QMiX+MA and QMiX+PUI groups in relation to S3 and S2, respectively. Final irrigation with QMiX associated with MA or PUI had superior antibacterial efficacy compared to EDTA, eliminating 100% of E. coli and E. faecalis strains. In addition, QMiX+PUI reduced 97.61% of the initial content of LPS.


Resumo O presente estudo objetivou comparar a eficácia do QMiX e do EDTA 17% associado à irrigação ultrassônica passiva (PUI) ou agitação manual (MA) na redução de E. faecalis, E. coli e LPS de canais radiculares. Quarenta dentes humanos unirradiculares foram divididos aleatoriamente em quatro grupos (n = 10), de acordo com o protocolo final de irrigação: EDTA+MA, QMiX+MA, EDTA+PUI, QMiX+PUI. Coletas das amostras foram obtidas a partir do conteúdo do canal radicular imediatamente antes do preparo (inicial-S1), após a instrumentação (S2), após o protocolo final de irrigação (S3) e 7 dias após a instrumentação e irrigação final (S4). A eficácia antimicrobiana e o conteúdo de endotoxina foram analisados ​​por procedimento de cultura (UFC/mL) e ensaio LAL (EU/mL), respectivamente. Os resultados foram analisados estatisticamente pelo teste de Kruskal-Wallis e Friedman (α = 5%). QMiX+MA e QMiX+PUI reduziram 100% das bactérias E. coli e E. faecalis e também preveniram a recolonização de E. faecalis em S4. O EDTA reduziu significativamente E. coli, mas não foi eficaz na redução de E. faecalis. Todos os protocolos reduziram EU/mL quando comparados com S1, no entanto, no S4 houve uma redução significativa de EU/mL apenas nos grupos QMiX+MA e QMiX+PUI em relação a S3 e S2, respectivamente. A irrigação final com QMiX associada a MA ou PUI apresentou eficácia antibacteriana superior em relação ao EDTA, eliminando 100% das cepas de E. coli e E. faecalis. Além disso, QMiX+PUI reduziu 97,61% do conteúdo inicial de LPS.


Subject(s)
Humans , Root Canal Irrigants , Dental Pulp Cavity , Sodium Hypochlorite , Ultrasonics , Edetic Acid , Root Canal Preparation , Endotoxins , Escherichia coli , Therapeutic Irrigation
19.
Braz. arch. biol. technol ; 62: e19180716, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055372

ABSTRACT

Abstract In a recent study, the treatment of different human cancer cell lines in vitro with ethylene diamine tetra-acetic acid (EDTA) showed a promising anticancer activity which could be a novel promising approach for cancer treatment. The aim of this study is to address the ability of EDTA to enhance the antitumor efficacy of the low dose of cisplatin (Cis) treatment in Ehrlich ascetic carcinoma (EAC) bearing mice. Sixty female albino mice were divided into six groups. The 1st group of mice was served as a negative control. 2nd - 6th groups were inoculated intraperitoneal (i.p) with 2×106 EAC cells/mouse. After one day of inoculation, the 2nd, 3rd and 4th groups were injected daily for 6 days (early treatment) with phosphate buffer saline, low dose of Cis and Cis/EDTA, respectively. After six days, the 5th and 6th groups were injected with the low dose of Cis and Cis/EDTA for 6 consecutive days (late treatment), respectively. At day 14, all groups of mice were sacrificed, sera were collected for biochemical assessment, then tumor volumes, counts, live and dead cells were determined from all groups. The results showed that EDTA co-treatment enhanced the efficacy of low dose of Cis at early and late time points. In addition, EDTA co-treatment potentially ameliorated the Cis-induced side effects on liver and kidney functions. In summary, co-therapy with EDTA could enhance the chemotherapeutic efficacy of low dose of Cis.


Subject(s)
Carcinoma, Ehrlich Tumor/drug therapy , Cisplatin/therapeutic use , Edetic Acid/administration & dosage , Treatment Outcome , Models, Animal , Mice , Antineoplastic Agents
20.
Braz. oral res. (Online) ; 33: e057, 2019. tab, graf
Article in English | LILACS | ID: biblio-1011660

ABSTRACT

Abstract The aim of this study was to evaluate the effect of peracetic acid (PAA) as a single irrigant on the smear layer, on the intraradicular dentinal bond strength, and on the penetrability of an epoxy-based resin sealer into the dentinal tubules. A total of 120 roots were distributed into 4 groups according to the irrigant used in root canal preparation: 1% PAA (PAA); 2.5% NaOCl followed by final irrigation with 17% EDTA and 2.5% NaOCl (NaOCl-EDTA-NaOCl); 2.5% NaOCl (NaOCl); and saline solution (SS). The smear layer was evaluated using scanning electron microscopy. The bond strength of an epoxy-based resin sealer (AH Plus) to root dentin was evaluated by the push-out test and penetrability of the sealer into dentinal tubules was observed by confocal laser microscopy. The results were analyzed by the Kruskal-Wallis and the Dunn post-test (α = 0.05). The use of 1% PAA as single root canal irrigant provided smear layer removal and improved the penetrability and bond strength of AH Plus to root dentin in a manner similar to that of the NaOCl-EDTA-NaOCl group (p > 0.05). The NaOCl and SS groups had higher values of smear layer and lower values of sealer penetrability and dentin bond strength than the PAA and NaOCl-EDTA-NaOCl groups (p < 0.05). Thus, 1% PAA has the potential to be used as a single irrigant in root canals.


Subject(s)
Humans , Peracetic Acid/chemistry , Root Canal Filling Materials/chemistry , Root Canal Irrigants/chemistry , Smear Layer/chemistry , Dental Pulp Cavity/drug effects , Dentin/drug effects , Epoxy Resins/chemistry , Reference Values , Sodium Hypochlorite/chemistry , Materials Testing , Microscopy, Electron, Scanning , Reproducibility of Results , Dental Bonding/methods , Edetic Acid/chemistry , Microscopy, Confocal , Dental Pulp Cavity/chemistry , Dentin/chemistry
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