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1.
Braz. j. biol ; 82: e233523, 2022. tab
Article in English | LILACS | ID: biblio-1153470

ABSTRACT

Abstract Microbiological studies of the sanitary and health status of psittacine birds that will be reintroduced is important in evaluating whether these animals act as carriers of pathogenic agents to other animals and humans. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a faster and more accurate method to identify bacteria than conventional microbiology methods. The aim of this study was to evaluate the health status of psittacines housed in captivity, by assessment of Gram-negative bacteria from fecal microbiota through MALDI- TOF MS identification. The results indicate high frequency of Gram-negative bacteria in feces (96.5%), especially from the Enterobacteriaceae family (88.7%). The most prevalent bacteria were Escherichia coli (39.0%), Proteus vulgaris (12.2%), Klebsiella spp. (12.1%) and Raoultella ornithinolytica (8.7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. and Escherichia hermannii were isolated with lower frequency. . All these agents are potentially pathogenic for parrots and can cause systemic infections in other animals and humans. These findings reinforce that MALDI- TOF MS proved to be a rapid and accurate method of identification of the microorganism and evaluation of the health status of psittacines, providing relevant data to assist decision-making regarding the sanitary protocols in wildlife centers, and possible future reintroduction of wild birds.


Resumo Estudos microbiológicos da sanidade de psitacídeos que serão reintroduzidos são importantes para avaliar se esses animais atuam como portadores de agentes patogênicos para outros animais e humanos. A espectrometria de massa por ionização/dessorção de matriz assistida por laser/tempo de vôo (MALDI-TOF MS) é um método mais rápido e preciso para identificar bactérias na comparação com métodos convencionais de microbiologia. O objetivo deste estudo foi avaliar o estado de saúde de psitacídeos cativos, identificando bactérias Gram-negativas da microbiota fecal por MALDI -TOF MS. Os resultados indicaram alta frequência de bactérias Gram-negativas nas fezes (96,5%), principalmente da família Enterobacteriaceae (88,7%). As mais prevalentes foram Escherichia coli (39,0%), Proteus vulgaris (12,2%), Klebsiella spp. (12,1%) e Raoultella ornithinolytica (8,7%). Proteus hauseri, Citrobacter spp., Morganella morgannii, Providencia rettgeri, Enterobacter spp. e Escherichia hermannii foram isolados com menor frequência. Todos esses agentes são potencialmente patogênicos para os papagaios e podem causar infecções sistêmicas em outros animais e seres humanos. Esses achados reforçam que o MALDI- TOF MS é um método rápido e preciso de identificação do microrganismo e avaliação do estado de saúde dos psitacídeos, fornecendo dados relevantes para auxiliar na tomada de decisões sobre os protocolos sanitários em centros de triagem de animais selvagens e sobre a possibilidade de reintrodução futura.


Subject(s)
Humans , Animals , Psittaciformes , Gram-Negative Bacteria , Proteus , Providencia , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Enterobacteriaceae
2.
Braz. j. biol ; 82: e237098, 2022. graf
Article in English | LILACS | ID: biblio-1153483

ABSTRACT

Abstract Endosymbiont bacteria can affect biological parameters and reduce the effectiveness of natural enemies in controlling the target insect. The objective of this work was to identify endosymbiont bacteria in Anaphes nitens (Girault, 1928) (Hymenoptera: Mymaridae), the main natural enemy used to manage Gonipterus platensis (Marelli, 1926) (Coleoptera: Curculionidae). Genomic DNA from six A. nitens populations was extracted and polymerase chain reactions (PCR) were performed with the primers to detect endosymbiont bacteria in this insect. The PCR products were amplified, sequenced, and compared with sequences deposited in the GenBank for the bacteria identification. All A. nitens populations had the bacterium Yersinia massiliensis (Enterobacteriales: Enterobacteriaceae). This bacterium was originally described as free-living, and it is associated with and composes part of the A. nitens microbiota. This is the first report of Y. massiliensis in an insect host.


Resumo As bactérias endossimbiontes podem afetar os parâmetros biológicos e reduzirem a eficácia de inimigos naturais no controle do inseto alvo. O objetivo deste trabalho foi identificar bactérias endossimbiontes em Anaphes nitens (Girault, 1928) (Hymenoptera: Mymaridae), o principal inimigo natural usado no manejo de Gonipterus platensis (Marelli, 1926) (Coleoptera: Curculionidae). O DNA genômico de seis populações de A. nitens foi extraído e as reações em cadeia da polimerase (PCR) realizadas com os primers para detectar bactérias endossimbiontes neste inseto. Os produtos de PCR foram amplificados, sequenciados e comparados com as sequências depositadas no GenBank para identificação das bactérias. Todas as populações de A. nitens tinham a bactéria Yersinia massiliensis (Enterobacteriales: Enterobacteriaceae). Esta bactéria foi originalmente descrita como de vida livre e está associada e compõe parte da microbiota de A. nitens. Este é o primeiro relato de Y. massiliensis em um hospedeiro.


Subject(s)
Animals , Weevils , Hymenoptera/genetics , Yersinia/genetics , Enterobacteriaceae/genetics
3.
Biomédica (Bogotá) ; 41(supl.2): 180-187, oct. 2021. tab, graf
Article in Spanish | LILACS | ID: biblio-1355769

ABSTRACT

Resumen | Introducción. La aparición de enterobacterias multirresistentes y productoras de betalactamasas de espectro extendido (BLEE) en pacientes de consulta externa con infecciones urinarias, representa un problema de salud pública en Perú. Objetivos. Caracterizar molecularmente enterobacterias multirresistentes aisladas de pacientes con diagnóstico de infección urinaria y procedentes de dos departamentos de la selva peruana. Materiales y métodos. Se hizo un estudio descriptivo, observacional y retrospectivo de 61 aislamientos de urocultivo procedentes de la selva peruana durante 2017 y 2018. Los perfiles de resistencia se identificaron utilizando el sistema automatizado MicroScan™ y para la detección de los genes blaTEM, blaCTX-M, blaSHV se empleó una reacción en cadena de la polimerasa (PCR) convencional. Resultados. Las enterobacterias positivas para BLEE más frecuentes por departamento fueron Escherichia coli en Madre de Dios (25 %, 10/40) y Ucayali (76,2 %, 16/21). En ambos departamentos, el gen blaCTX-Mfue el más frecuente (25/61; 41 %), seguido por blaTEM(15/61; 24,6 %) y blaSHV (10/61; 16,4 %). En el perfil de sensibilidad antimicrobiana, se detectó 72,6 % de resistencia contra ampicilina, 82,3 % contra cefalotina y 88,7 % contra nitrofurantoína. Conclusiones. El porcentaje de cepas de enterobacterias multirresistentes productoras de BLEE en ambos departamentos fue del 57,4 % y el gen bla CTX-M fue el más frecuente.


Abstract | Introduction. The emergence of multiresistant enterobacteria producing extended-spectrum beta-lactamase (ESBL) in outpatients with urinary tract infections represents a public health problem in Perú. Objectives. To characterize multiresistant enterobacteria isolated from patients diagnosed with urinary tract infection in two Peruvian jungle departments using molecular techniques. Materials and methods. We conducted a descriptive, observational, and retrospective study of 61 urine culture isolates from two departments in the Peruvian jungle during 2017-2018. Resistance profiles were identified using the MicroScan™ automated system and a conventional polymerase chain reaction (PCR) was used for the detection of blaCTX-M, blaTEMand blaSHV genes. Results. The most common positive ESBL enterobacteria for each department were Escherichia coli in Madre de Dios (10/40; 25%) and Ucayali (16/21; 76.2%). Gene blaCTX-Mwas the most prevalent in both departments (25/61; 41%), followed by blaTEM (15/61; 24.6%), and blaSHV (10/61; 16.4%). As for the antimicrobial susceptibility profile, we detected resistance levels of 72.6% for ampicillin, 82.3% for cephalothin, and 88.7% for nitrofurantoin. Conclusions. BLEE-producing multi-resistant enterobacteria strains in both departments were 57.4% and blaCTX-M was the most common gene.


Subject(s)
Drug Resistance, Microbial , Enterobacteriaceae , beta-Lactam Resistance , Genes
4.
Infectio ; 25(3): 193-196, jul.-set. 2021. tab, ilus
Article in Spanish | LILACS, COLNAL | ID: biblio-1250092

ABSTRACT

Resumen El constante aumento de Enterobacterales productores de carbapenemasas (CPE) se constituye en un problema de salud pública a nivel mundial, por el impacto generado en la mortalidad de los pacientes. El tracto gastrointestinal es el principal reservorio de este tipo de microorganismos, por lo cual, la colonización rectal se convierte en un importante factor de riesgo para el desarrollo de posteriores infecciones. Una de las estrategias de vigilancia epidemiológica activa, es la búsqueda de pacientes colonizados, a través de cultivos de tamización para detectar estos microrganismos multirresistentes. Reportamos el caso de un paciente, con historia de sepsis de origen pulmonar, colonizado por Klebsiella pneumoniae con coproducción de carbapenemasas NDM + KPC y Escherichia coli con carbapenemasa NDM. Este hallazgo es cada vez más frecuente, lo cual implica un reto en su detección y diagnóstico. Se describen características del paciente, procedimientos realizados y hallazgos microbiológicos.


Abstract The constant increase in carbapenemase-producing Enterobacterales (CPE) constitutes a public health problem worldwide, due to the impact generated on the mortality of patients. The gastrointestinal tract is the main reservoir for this microorganism, which is why, rectal colonization becomes an important risk factor for the development of subsequent infections. One of the active epidemiological surveillance strategies is the search for colonized patients through screening cultures, to detect these multi-resistant microorganisms. We report the case of a patient, with a history of sepsis of pulmonary origin, colonized by Klebsiella pneumoniae with co-production of NDM + KPC carbapenemases and NDM carbapenemase-producing Escherichia coli. This finding is more and more frequent, which implies a challenge in its detection and diagnosis. Patient characteristics, procedures performed and microbiological findings are described.


Subject(s)
Humans , Middle Aged , Enterobacteriaceae , Carbapenem-Resistant Enterobacteriaceae , Sepsis , Gastrointestinal Tract , Escherichia coli , Infections , Klebsiella pneumoniae
5.
Infectio ; 25(2): 89-92, abr.-jun. 2021. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1250073

ABSTRACT

Resumen Objetivo: Detectar la presencia de Enterobacterias productoras de carbapenemasas en hisopados rectales de neonatos mediante técnica de nefelometría láser y caracterización del tipo de carbapenemasa mediante test inmunocromatográfico. Materiales y Métodos: Estudio descriptivo de corte transversal. Fueron incluidos 57 neonatos, tamizados al ingreso a UCI, mediante hisopado rectal, procesado por nefelometría laser HB&L Carbapenemase (Alifax®) y caracterización del tipo de carbapenemasa por inmunocromatografía rápida RESIST-3 (Coris BioConcept®). Resultados: Encontramos un alto porcentaje de colonización rectal (22.9%) correspondiente a 13 hisopados positivos y 44 (77.1%) fueron negativos por nefelometría láser. Por VITEK 2® se obtuvo identificación de Klebsiella pneumoniae resistente a carbapenémicos en los 13 aislamientos y el test inmunocromatográfico reveló la presencia de carbapenemasas blaKPC en estos aislamientos. Discusión: Estudios evidencian el aumento de la colonización por microorganismos productores de carbapenemasas en neonatos. Los resultados de este estudio demuestran que un porcentaje significativo de neonatos que ingresan a las Unidades de Cuidado Neonatal se encuentran colonizados con Enterobacterias productoras de carbapenemasas en tracto intestinal. Lo anterior constituye un riesgo potencial para su diseminación y posterior desarrollo de brotes, en donde surge la importancia de implementar estrategias de vigilancia activa como la tamización rectal para la detección oportuna de neonatos colonizados.


Abstract Objective: To detect the presence of carbapenemase-producing Enterobacteriaceae in rectal swabs of neonates by means of laser nephelometry technique and characterization of the type of carbapenemase by immunochromatographic test. Materials and Methods: Descriptive cross-sectional study. 57 neonatal patients were included; They underwent rectal screening upon admission to the ICU, using swabs which were processed by HB&L Carbapenemase laser nephelometry (Alifax®) and characterization of the type of carbapenemase by RESIST-3 rapid immu nochromatography (Coris BioConcept®). Results: We found a high percentage of rectal colonization (22.9%) corresponding to 13 positive swabs and 44 samples (77.1%) were negative by laser nephelome try. Identification of carbapenem-resistant Klebsiella pneumoniae was obtained by VITEK 2® in the 13 isolates and the immunochromatographic test revealed the presence of blaKPC carbapenemases in these isolates. Discussion: Studies show increased colonization by carbapenemase-producing microorganisms in neonates. The results of this study demonstrate that a significant percentage of neonates who enter Neonatal Care Units are colonized with Enterobacteriaceae that produce carbapenemases in the intestinal tract. This constitu tes a potential risk for its spread and subsequent development of outbreaks, where the importance of implementing active surveillance strategies such as rectal screening for the timely detection of colonized neonates arises.


Subject(s)
Humans , Male , Female , Infant, Newborn , Carbapenems , Diagnostic Techniques and Procedures , Enterobacteriaceae , Mass Screening , Cross-Sectional Studies , Watchful Waiting , Intensive Care Units , Nephelometry and Turbidimetry
6.
Chinese Journal of Biotechnology ; (12): 1092-1106, 2021.
Article in Chinese | WPRIM | ID: wpr-878616

ABSTRACT

Antibiotic resistance is a major global concern and challenge in the 21st century. Enterobacteriaceae are one of the important pathogens of hospital-acquired infections. With the increasing use of antibiotics in clinical practice, a variety of drug-resistant Enterobacteriaceae, especially multidrug-resistant Enterobacteriaceae have emerged, posing an increasingly serious threat to human health. Bacteria can acquire antibiotic resistance by mutation or horizontal transfer of antibiotic resistance genes, and it is often possible to predict the corresponding resistance phenotype from known mechanism. However, recent findings suggest that genetic background and environmental factors could alter the expression of specific resistance genes and that a given genotype does not always generate the expected resistance phenotype. The genotype-phenotype segregation greatly hampers our ability to predict antibiotic resistance phenotype from a genetic perspective. In this review, we explore the genetic and environmental regulation of the expression of antibiotic resistance genes in a variety of Enterobacteriaceae, with the aim to provide scientific evidence for genetic prediction of antibiotic resistance phenotype and clinical guidance on drug use.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bacteria , Cross Infection , Drug Resistance, Microbial , Enterobacteriaceae/genetics , Humans
7.
Chinese Journal of Biotechnology ; (12): 1081-1091, 2021.
Article in Chinese | WPRIM | ID: wpr-878615

ABSTRACT

The enterobacterial common antigen (ECA) is a polysaccharide composed of polysaccharide repeats that are located in the outer membrane of almost all Enterobacteriaceae bacteria and has diverse biological functions. ECA is synthesized by the synergistic action of multiple genes that are present in clusters on the genome of Enterobacteriaceae bacteria, forming the ECA antigen gene cluster, an important virulence factor that plays a role in host invasion and survival of Enterobacteriaceae in vivo. ECA also plays an important role in the maintenance of the bacterial outer membrane permeability barrier, flagella gene expression, swarming motility, and bile salts resistance. In addition, ECALPS, anchored in the core region of bacterial lipopolysaccharide, is an important surface antigen for bacteria, stimulating high levels of antibody production in the host and could be a target for vaccine research. This review summarizes ECA purification, genes involved in ECA biosynthesis, its immunological characteristics, biological functions and clinical applications.


Subject(s)
Antigens, Bacterial/genetics , Enterobacteriaceae/genetics , Lipopolysaccharides , Polysaccharides
8.
Pesqui. vet. bras ; 41: e06696, 2021. tab
Article in English | LILACS, VETINDEX | ID: biblio-1340354

ABSTRACT

The grey-breasted parakeet (Pyrrhura griseipectus) is an endangered psittacine species that have been affected by illegal trade and deforestation. Currently, this endemic species is only found in three areas in Ceará state, in Brazil. This study aimed to investigate the frequency and diversity of Enterobacteriaceae in wild adult grey-breasted parakeets and determine their susceptibility to antimicrobial agents. Cloacal swab samples were collected from 27 individuals and environmental swabs (drag swabs) from five nests used by these birds. Twenty-seven strains from nine species of Enterobacteriaceae were recovered from cloacal swabs, and the most prevalent bacteria strains were Hafnia alvei (22%) and Pantoea agglomerans (22%). From environmental nest samples, seven strains from three bacterial species were isolated, being the P. agglomerans the most frequent species (100%). Twenty-two of the 27 isolates (81.4%) exhibited antibiotic resistance, varying from one to eight of the 12 antimicrobials commonly used. Resistance to amoxicillin was the most prevalent (70.4%), followed by azithromycin (22.2%) and ceftriaxone (18.5%). None of the strains were resistant to gentamicin, tobramycin, ciprofloxacin or tetracycline. The H. alvei was the main species presenting multidrug resistance, including resistance against meropenem, which is an important finding. These results could provide interesting information on the health of these endangered wild grey-breasted parakeets. They could also indicate that the obtained isolates are part of a group of bacteria that are typical components of the enteric microbiota of birds, which present elevated rates of resistance to amoxicillin.(AU)


O periquito-de-cara-suja (Pyrrhura griseipectus) é uma espécie de psitacídeo considerado pela IUCN como ameaçado de extinção, resultado do comércio ilegal e do desmatamento. Atualmente, essa espécie endêmica é encontrada apenas em três áreas no estado do Ceará, Brasil. O objetivo deste estudo foi investigar a frequência e a diversidade de Enterobacteriaceae em periquitos de peito cinza adultos selvagens e determinar sua suscetibilidade a agentes antimicrobianos. Amostras de suabes cloacais foram coletadas de 27 indivíduos e de suabes ambientais (suabes de arrasto) de cinco ninhos utilizados por essas aves. Vinte e sete cepas de nove espécies de Enterobacteriaceae foram isoladas a partir de suabes cloacais, sendo as cepas bacterianas mais prevalentes Hafnia alvei (22%) e Pantoea agglomerans (22%). Das amostras ambientais de ninhos foram isoladas sete linhagens de três espécies bacterianas, sendo P. agglomerans a espécie mais frequente (100%). Vinte e dois dos 27 isolados (81,4%) exibiram resistência a antibióticos, variando de um a oito dos 12 antimicrobianos comumente usados. A resistência a amoxicilina foi a mais prevalente (70,4%), seguida por azitromicina (22,2%) e ceftriaxona (18,5%). Nenhuma das cepas era resistente à gentamicina, tobramicina, ciprofloxacina ou tetraciclina. H. alvei foi a principal espécie que apresentou resistência a múltiplas drogas e que também esteve associada a um outro achado relevante desta pesquisa, que foi a detecção de um caso de resistência ao meropenem. Esses dados fornecem informações relevantes sobre a saúde desses periquitos selvagens ameaçados e permite concluir que os isolados obtidos fazem parte de um grupo de bactérias que normalmente compõe a microbiota entérica das aves, sendo a amoxicilina envolvida em elevadas taxas de resistência.(AU)


Subject(s)
Animals , Parakeets/microbiology , Parrots/microbiology , Enterobacteriaceae/isolation & purification , Animals, Wild , Enterobacteriaceae Infections , Anti-Infective Agents/analysis
9.
Rev. Soc. Bras. Med. Trop ; 54: e02622020, 2021.
Article in English | ColecionaSUS, LILACS, ColecionaSUS, SES-SP | ID: biblio-1143877

ABSTRACT

Abstract INTRODUCTION: Carbapenemase-resistant enterobacteria that produce the bla NDM gene are found worldwide. However, this is the first report of blaNDM in Klebsiella aerogenes in Brazil. METHODS: The identification of bacterial species was performed using anautomated system and confirmed by biochemical tests, 16S rRNA gene sequencing, and detection of resistance genes. RESULTS: The clinical isolate showed minimum inhibitory concentration resistance to meropenem and polymyxin B at 8mg/L and 4mg/L, respectively. Only the blaNDM gene was detected. CONCLUSIONS: The current report of the blaNDM gene in isolated MDR enterobacteria indicates that this gene can spread silently in a hospital setting.


Subject(s)
Enterobacter aerogenes/genetics , Bacterial Proteins , beta-Lactamases/genetics , Brazil , RNA, Ribosomal, 16S , Microbial Sensitivity Tests , Drug Resistance, Multiple, Bacterial/genetics , Enterobacteriaceae , Klebsiella pneumoniae/genetics , Anti-Bacterial Agents/pharmacology
10.
Rev. patol. trop ; 50(4)2021. ilus
Article in English | LILACS | ID: biblio-1353224

ABSTRACT

okenella regensburgei belongs to the family Enterobacteriaceae and is an opportunistic agent rarely associated with infections in humans. We report a case of osteoarticular knee infection caused by Y. regensburgei in a patient under treatment for rheumatoid arthritis, using corticosteroids, with complication in primary total arthroplasty of the knee. Y. regensburgei was identified using the VITEK2 system. Antimicrobial susceptibility testing was performed using the disk-diffusion method, according to the guidelines from the Clinical and Laboratory Standards Institute. The patient presented favorable clinical evolution after the second debridement, with complete removal of the prosthesis and antibiotic therapy with sulfamethoxazole/trimethoprim. This is the first case of Y. regensburgei infection described d in Brazil.


Subject(s)
Arthritis, Rheumatoid , Sulfamethoxazole , Trimethoprim , Osteoarthritis, Knee , Enterobacteriaceae , Knee
11.
Rev. méd. Minas Gerais ; 31: 31105, 2021.
Article in Portuguese | LILACS | ID: biblio-1291252

ABSTRACT

Introdução: A cesárea é realizada em condições maternas e/ou fetais que impeçam o nascimento via vaginal. Devido ao risco de complicações infecciosas, o procedimento é precedido de antibioticoprofilaxia, o que pode provocar a seleção de cepas multirresistentes e alterar a flora nativa do indivíduo. O colostro é um fator determinante para a colonização do trato digestivo por ser fonte probiótica, além de apresentar papel importante na modulação do sistema imunológico e desenvolvimento do neonato. Objetivo: Este estudo visa avaliar possíveis interferências da antibioticoprofilaxia realizada previamente à cesárea na flora materna. Métodos: Selecionou-se 140 lactantes após aplicação dos critérios de exclusão, divididas em grupos estudo (realizaram parto cesáreo com antibioticoprofilaxia) e controle (realizaram parto vaginal sem antibioticoprofilaxia) para coleta das amostras ­ colostro e esfregaço areolar ­ com intuito de realizar a análise microbiológica das mesmas no Laboratório de Microbiologia da Faculdade de Medicina de Barbacena. Resultados: 94 lactantes pertencem ao grupo estudo e 46 ao controle. O microrganismo mais prevalente em todas as amostras foi a Candida sp. Ao se discriminar os grupos, a mesma também apresentou maior prevalência, seguida de Enterococcus sp. Em relação à detecção de Staphylococcus aureus pelo esfregaço mamilar, ele foi encontrado em 24 amostras do grupo controle e em 34 do grupo estudo. Na análise comparativa da prevalência de microrganismos entre os grupos, não se observou diferença estatisticamente relevante. Conclusão: A antibioticoprofilaxia peri-operatória mostrou-se segura em não alterar a composição da flora materna. Entretanto, mais estudos sobre o tema devem ser realizados.


Introduction: The C-section is performed under maternal and / or fetal conditions that prevent vaginal birth. Because of the infectious complications, the procedure is preceded by antibiotic prophylaxis, which can cause the selection of multidrug-resistant strains and shift the individual's native flora. Colostrum is a determining factor for colonization of the digestive tract being a probiotic source, along with playing an important role in the modulation of the immune system and development of the newborn. Objective: This study aims to assess possible interferences of antibiotic prophylaxis performed prior to cesarean section on maternal flora. Methods: 140 lactating women were selected after applying the exclusion criteria, divided into study groups (94 lactating women who underwent cesarean delivery with antibiotic prophylaxis) and control groups (46 lactating women who underwent vaginal delivery without antibiotic prophylaxis) for sample collection - colostrum and areolar swab - therefore perform the microbiological analysis at the Laboratório de Microbiologia da Faculdade de Medicina de Barbacena. Results: The most prevalent microorganism in all samples was Candida sp. Discriminating the groups, it also had the highest prevalence, followed by Enterococcus sp. Regarding the detection of Staphylococcus aureus by the areolar swab, it was found in 24 samples from the control group and 34 from the study group. In the comparative analysis of prevalence of microorganisms, between the groups, there was no statistically significant difference. Conclusion: Perioperative antibiotic prophylaxis proved to be safe not changing the composition of the maternal flora. However, further studies on the subject should be carried out.


Subject(s)
Humans , Pregnancy , Microbiological Techniques , Colostrum , Infant , Staphylococcus aureus , Candida , Cesarean Section , Flora , Antibiotic Prophylaxis , Parturition , Postpartum Period , Enterobacteriaceae , Gastrointestinal Microbiome
12.
Rev. Soc. Bras. Med. Trop ; 54: e0724-2020, 2021. tab
Article in English | LILACS | ID: biblio-1155606

ABSTRACT

Abstract INTRODUCTION: Inadequate wastewater treatment and fecal contamination have a strong environmental impact on antimicrobial resistance (AMR). This study evaluated the profile of AMR enterobacteria and fecal contamination from four surface waters: Jiquiriça-Brejões River and Cabrito, Tororó, and Abaeté Lagoons. METHODS: We analyzed AMR β-lactamase genes using the polymerase chain reaction method and fecal contamination using Coliscan®. RESULTS: We found high levels of fecal contamination, β-lactamase producers, and AMR genes (blaOXA-48, blaSPM, and blaVIM) in all waterbodies. CONCLUSIONS: Poor sanitation evidenced by fecal contamination and human activities around these surface waters contributed to the distribution and increase in AMR enterobacteria.


Subject(s)
Humans , Enterobacteriaceae/genetics , Anti-Infective Agents , Rural Population , Uganda , Feces
13.
Rev. Soc. Bras. Med. Trop ; 54: e0864-2020, 2021. tab
Article in English | LILACS | ID: biblio-1155547

ABSTRACT

Abstract Proteus mirabilis is one of the main pathogens causing urinary tract infections and sepsis. To our knowledge, this is the first report of a P. mirabilis hosting bla GES. The presence of these genes was determined using PCR and sequencing. We identified the presence of bla GES-1 in all three isolates. In addition, we identified the bla KPC-2 and bla NDM-1 genes in the two strains. These data emphasize the importance of monitoring and surveillance of all enterobacteria. The circulation of P. mirabilis strains carrying bla GES-1 constitutes a new scenario of resistance in this species and should be an epidemiological alert for global health.


Subject(s)
Proteus mirabilis/genetics , beta-Lactamases/genetics , Microbial Sensitivity Tests , Enterobacteriaceae , Anti-Bacterial Agents
14.
Arq. bras. med. vet. zootec. (Online) ; 72(6): 2365-2372, Nov.-Dec. 2020. tab
Article in English | LILACS, VETINDEX | ID: biblio-1142322

ABSTRACT

The objective of this study was to evaluate the effects of probiotics and synbiotics on the performance and Enterobacteriaceae count of broiler chickens. A total of 640 one-day-old male broiler chicks were distributed in a completely randomized design with four treatments and eight replicates with 20 birds each. The treatments were: ration with performance enhancer (zinc bacitracin; positive control); ration without performance enhancer and probiotic/synbiotic (negative control); ration with probiotics; and ration with synbiotics. At 35 days, five birds from each treatment were euthanized and intestinal contents were harvested for determining the Enterobacteriaceae count. The performance data and average colony-forming units (CFUs) transformed as log CFU/g were subjected to analysis of variance and Tukey's test. The effects of probiotics and synbiotics were observed in the initial phase, with supplemented birds exhibiting comparable weight gain to those supplemented with bacitracin. No effect of the treatment on broiler performance was observed after 42 days. The enterobacterial count was comparable among all experimental treatments. Supplementation with probiotics and synbiotics did not compromise the performance of broilers and did not alter the Enterobacteriaceae count.(AU)


Objetivou-se avaliar o efeito do probiótico e do simbiótico sobre o desempenho e a contagem de Enterobacteriaceae em frangos. Foram utilizados 640 pintos de corte, machos, de um dia de idade, distribuídos em delineamento inteiramente ao acaso, com quatro tratamentos, oito repetições com 20 aves cada. Os tratamentos foram: ração com melhorador de desempenho (bacitracina de zinco) (controle positivo); ração sem melhorador de desempenho e sem probiótico/simbiótico (controle negativo); ração com probiótico e ração com simbiótico. Aos 35 dias, cinco aves por tratamento foram eutanasiadas para retirada de conteúdo intestinal e determinação de Enterobacteriaceae. As médias das unidades formadoras de colônias, transformadas em log/UFC/g, e de desempenho foram submetidas à análise de variância e comparadas pelo teste Tukey. Foi observado efeito do probiótico e do simbiótico na fase inicial, sendo que aves apresentaram os mesmos resultados de ganho de peso e de peso corporal que o grupo de aves alimentado com bacitracina. Aos 42 dias, não houve efeito dos tratamentos sobre o desempenho. Aves que não receberam nenhum aditivo não apresentaram maior contagem de enterobactérias, sendo semelhantes aos demais tratamentos. A adição do probiótico e do simbiótico não compromete o desempenho dos frangos e não altera a contagem de Enterobacteriaceae.(AU)


Subject(s)
Animals , Bacitracin/administration & dosage , Chickens/microbiology , Escherichia coli Infections/drug therapy , Enterobacteriaceae , Escherichia coli , Microbiota
15.
Gac. méd. Méx ; 156(6): 604-609, nov.-dic. 2020. tab
Article in Spanish | LILACS | ID: biblio-1249973

ABSTRACT

Resumen Introducción: Existe poca información acerca de la efectividad de las combinaciones ceftolozano/tazobactam y ceftazidima/avibactam en cepas clínicamente relevantes aisladas en México. Objetivo: Determinar el perfil antimicrobiano de ambos antibióticos en nuestra comunidad. Método: El presente estudio de investigación fue prospectivo, descriptivo y transversal. Se incluyeron cepas clínicamente relevantes aisladas a partir de cultivos de cepa pura durante el periodo de agosto de 2018 a enero de 2019 en Mexicali, Baja California, México. Resultados: Se analizaron 74 cepas de enterobacterias y 19 cepas de Pseudomonas aeruginosa; el porcentaje de sensibilidad de ceftazidima/avibactam fue de 100 % contra enterobacterias y de 72.7 % contra Pseudomonas aeruginosa; el porcentaje de sensibilidad de ceftolozano/tazobactam fue de 90.5 % para enterobacterias y de 72.7 % para Pseudomonas aeruginosa. Conclusiones: Las combinaciones ceftolozano/tazobactam y ceftazidima/avibactam ofrecen buena sensibilidad antimicrobiana in vitro, tanto contra enterobacterias productoras de betalactamasas de espectro extendido como contra Pseudomonas aeruginosa. Se requieren más datos para valorar la respuesta clínica en pacientes que reciben esas combinaciones de antibióticos.


Abstract Introduction: There is limited information on the effectiveness of ceftolozane/tazobactam and ceftazidime/avibactam combinations on clinically relevant strains isolated in Mexico. Objective: To determine the antimicrobial profile of both antibiotic combinations in our community. Method: The present research study was prospective, descriptive and cross-sectional. Clinically relevant strains isolated from pure-strain cultures were included during the period from August 2018 to January 2019 in Mexicali, Baja California, Mexico. Results: 74 enterobacteriaceae and 19 Pseudomonas aeruginosa strains were analyzed; the percentage of sensitivity of ceftazidime/avibactam was 100 % for enterobacteriaceae and 72.7 % for Pseudomonas aeruginosa; the percentage of sensitivity of ceftolozane/tazobactam for enterobacteriaceae was 90.5 % and 72.7 % for Pseudomonas aeruginosa. Conclusions: The ceftolozane/tazobactam and ceftazidime/avibactam combinations offer good antimicrobial sensitivity in vitro, both for ESBL-producing enterobacteriaceae and Pseudomonas aeruginosa. More data are required to assess clinical response in patients receiving these antibiotic combinations.


Subject(s)
Humans , Pseudomonas aeruginosa/drug effects , Ceftazidime/therapeutic use , Cephalosporins/therapeutic use , Enterobacteriaceae/drug effects , Azabicyclo Compounds/therapeutic use , Anti-Bacterial Agents/therapeutic use , Pseudomonas aeruginosa/isolation & purification , Microbial Sensitivity Tests , Cross-Sectional Studies , Prospective Studies , Drug Combinations , Enterobacteriaceae/isolation & purification , Tazobactam/therapeutic use , Mexico
16.
Pesqui. vet. bras ; 40(10): 804-813, Oct. 2020. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1143408

ABSTRACT

Bacterial resistance is shown to be an inevitable side effect due to the excessive use of antibiotics, becoming a significant concern worldwide. Knowledge of regional bacterial resistance profiles enables the development of site-specific infection control practices, making conscious and moderate use of commercially available antibiotics. The aim of this study was the retrospective evaluation of the antimicrobial resistance profile of bacteria isolated from companion animal infections in the region of Umuarama/PR, from 2013 to 2017. This research was performed by analyzing the database belonging to the "Laboratório de Microbiologia Animal" at the "Universidade Estadual de Maringá" (UEM). Staphylococcus spp. represented 45.53% of the bacteria isolated from clinical infections in small animals in the period and place evaluated, followed by enterobacteria (34.04%), non-fermenting Gram-negative bacilli (NFGNB, 11.06%) and Streptococcus/Enterococcus (9.36%). A high number of antimicrobial resistance to antibiotics used in veterinary medicine was found. The lowest resistances associated with the best impact factor values were found for aminoglycosides, especially amikacin, chloramphenicol, and fluoroquinolones (norfloxacin and ciprofloxacin). Intermediate results were found for sulbactam-associated ampicillin, ceftriaxone, amoxicillin-clavulanic acid, and enrofloxacin. According to the number of resistant antimicrobial drugs, 64.26% (151/235) of the isolates were classified as multidrug-resistant, being 15.32% extensively resistant. Considering the resistance to antimicrobial classes, 68.94% (162/235) of the isolates were classified as multiresistant, being 19.15% extensively resistant. No bacterial strains were characterized as pan-resistant, but ten bacteria were resistant to all classes tested, with isolated susceptibility to certain drugs. Through the evaluation of resistance profiles found in the period and place studied and relevant literature, it is clear that there is a growing increase in the number of multiresistant bacteria among domestic animals which characterizes a serious risk to public health. The therapeutic arsenal is becoming increasingly diminished, and there is more difficulty in empirical drug selection, making antimicrobial susceptibility testing essential for more specific selection in antimicrobial therapy. Educational measures on the conscious use of antibiotics, infection control, and prevention of local specific zoonoses need to be instituted for the knowledge of health professionals and general access of the population.(AU)


A resistência bacteriana, mostra-se como um efeito colateral inevitável pelo excessivo uso de antibióticos, tornando-se alvo de grande preocupação mundial. O conhecimento dos perfis de resistência bacteriana regionais possibilita o desenvolvimento de práticas de controle de infecções específicas para cada localidade, fazendo uso consciente e moderado dos antibióticos disponíveis no mercado. O objetivo deste estudo foi a avaliação retrospectiva do perfil de resistência antimicrobiana de bactérias isoladas de infecções de animais de companhia na região de Umuarama/PR, no período de 2013 a 2017. Esta pesquisa foi realizada por meio da análise do banco de dados pertencente ao Laboratório de Microbiologia Animal da Universidade Estadual de Maringá (UEM). Os Staphylococcus spp. representaram 45,53% das bactérias isoladas de infecções clínicas em pequenos animais no período e local avaliado, seguido por enterobactérias (34,04%), bacilos Gram-negativos não fermentados (BGNNF, 11,06%) e Streptococcus/Enterococcus (9,36%). Um número elevado de resistência antimicrobiana frente aos antibióticos utilizados na medicina veterinária foi encontrado. As menores resistências associadas aos melhores valores do fator de impacto foram encontrados para aminoglicosídeos, em especial amicacina, cloranfenicol, fluoroquinolonas (norfloxacina e ciprofloxacina). Já resultados intermediários foram encontrados para ampicilina associada a sulbactam, ceftriaxona, amoxacilina com ácido clavulônico e enrofloxacina. Conforme o número de drogas antimicrobianas resistentes, foram classificados como multirresistentes 64,26% (151/235) dos isolados, sendo 15.32% extensivamente resistentes. Já considerando a resistência a classes de antimicrobianos, 68,94% (162/235) dos isolados foram classificados como multirresistentes, sendo 19.15% extensivamente resistentes. Nenhum isolado bacteriano foi caracterizado como pan-resistente, porém 10 bactérias foram resistentes a todas as classes testadas, com susceptibilidade isolada a determinadas drogas. Por meio da avaliação dos perfis de resistência encontrados no período e local estudados e de literatura pertinente, percebe-se que há um aumento crescente no número de bactérias multirresistentes entre os animais domésticos o que caracteriza um grave risco à saúde pública. O arsenal terapêutico está se tornando cada vez mais diminuto e há mais dificuldade na seleção empírica de drogas, tornando essencial a realização de testes de susceptibilidade antimicrobiana para uma seleção mais específica na terapêutica antimicrobiana. Medidas educativas sobre o uso consciente dos antibióticos, controle de infecções e prevenção de zoonoses específicas para as localidades precisam ser instituídas para conhecimento dos profissionais do setor da saúde e acesso geral da população.(AU)


Subject(s)
Animals , Cats , Dogs , Staphylococcus/drug effects , Enterococcus/drug effects , Drug Resistance, Multiple, Bacterial , Enterobacteriaceae/drug effects
17.
Rev. colomb. cardiol ; 27(5): 434-445, sep.-oct. 2020. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1289254

ABSTRACT

Resumen Objetivo: describir las características epidemiológicas, clínicas y microbiológicas de los pacientes que cursan con miocarditis por Enterobacterias. Métodos: se realizó una revisión sistemática de la literatura, en la que se incluyeron Pubmed, Ovid, Scopus, SciELO y LILACS sin exclusión por tipo de idioma. La población objetivo de estudio fueron los pacientes con diagnóstico de infección bacteriana por bacilo gram negativo mediante cultivo, técnicas moleculares o histopatología, y quienes presentaban biopsia de miocardio o, en su defecto, resonancia magnética cardiaca con hallazgos sugestivos de miocarditis. Resultados: se encontraron 742 artículos, de los cuales se incluyeron 24; en estos se reportaron 27 pacientes. La edad promedio fue de 31 años. El 81% de los pacientes eran de sexo masculino. El síntoma principal fue diarrea (80%), seguido de fiebre (53%) y dolor torácico (38%). El 37% de los pacientes fallecieron. El hallazgo más común en el electrocardiograma fue la elevación del segmento ST (36,7%). En quienes se realizó ecocardiograma se encontraron anormalidades en 50% de los casos, siendo más frecuente la disminución en la fracción de eyección. El microorganismo más común fue el Campylobacter jejuni, seguido por Salmonela sp. Conclusiones: la miocarditis causada por enterobacterias es más frecuente en pacientes adultos jóvenes de sexo masculino. Los síntomas gastrointestinales suelen estar presentes al momento de la presentación clínica. El diagnóstico requiere de alta sospecha clínica teniendo en cuenta que las anormalidades eléctricas y en ecocardiograma no se encuentran en todos los pacientes.


Abstract Objective: To describe the epidemiological, clinical, and microbiological characteristics of patients with myocarditis due to Enterobacteria. Methods: A systematic review was carried out on the literature, which included Pubmed, Ovid, Scopus, SciELO, and LILACS, with no exclusions due to language. The target population of the study were patients with a diagnosis of bacterial infection due to gram negative bacillus by means of a culture, or using molecular or histopathology technique. They also had to have had a myocardial biopsy or, if not, a cardiac magnetic resonance scan with findings suggestive of myocarditis. Results: Out of a total of 742 articles found, 24 of these, in which 27 patients were described, were included. The mean age was 31 years, and 81% were male. The main symptom was diarrhoea (80%), followed by fever (53%), and chest pain (38%). More than one-third (37%) of the patients died. The most common finding on the electrocardiogram (ECG) was elevation of the ST segment (36.7%). Abnormalities were found in 50% of the cases, on whom a cardiac ultrasound was performed, with a decrease in the ejection fraction being the most common. The most common microorganism was Campylobacter jejuni, followed by Salmonella spp. Conclusions: Myocarditis caused by enterobacteria is most common in young male patients. The gastrointestinal symptoms are usually present from the clinical onset. The diagnosis requires a high clinical suspicion, taking into account that the abnormalities in the ECG and cardiac ultrasound are not found in all patients.


Subject(s)
Humans , Male , Adult , Salmonella , Shigella , Enterobacteriaceae , Myocarditis , Vibrio , Yersinia , Campylobacter , Clostridium
18.
Pesqui. vet. bras ; 40(9): 690-695, Sept. 2020. tab
Article in English | LILACS, VETINDEX | ID: biblio-1143420

ABSTRACT

Plasmid-mediated polymyxin resistance was first described in 2015, in China, in Escherichia coli carrying the mcr-1 (Mobile Colistin Resistance-1) gene. Since then, it has become a major public health challenge worldwide, representing a major threat to human and animal health. In addition, there are still few reports on the prevalence of mcr-1 in Enterobacteriaceae isolated from humans, animals and food. Therefore, the purpose of the study was to investigate the occurrence of the mcr-1 gene in bacterial isolates with phenotypic resistance to polymyxin B obtained from clinical specimens of companion animals. Phenotypic resistance to polymyxin B were determined by broth microdilution and the susceptibility profile to other antimicrobials (amikacin, amoxicillin/clavulanate, ampicillin, ampicillin/sulbactam, aztreonam, cefazolin, cefepime, cefotaxime, cefoxitin, ceftazidime, ceftriaxone, chloramphenicol, ciprofloxacin, doxycycline, ertapenem, gentamicin, imipenem, marbofloxacin, meropenem, phosphomycin, piperacillin/tazobactam, tetracycline, ticarcillin/clavulanate, tobramycin and trimethoprim/sulfamethoxazole) by disc-diffusion agar method. The extraction of bacterial DNA was performed via heat shock followed by spectrophotometric evaluation. To verify the presence of mcr-1, the Polymerase Chain Reaction was employed using specific primers, followed by agarose gel electrophoresis. The positive isolates had the corresponding amplicons sequenced. In this study, there were identified the first isolates of Escherichia coli, Klebsiella spp. and Enterobacter spp. carrying the mcr-1 gene derived from specimens of companion animals in Brazil. Our results suggest the dissemination of resistance to polymyxins in the community and the environment, highlighting the need for surveillance and optimized treatment guidelines.(AU)


A resistência à polimixina mediada por plasmídeo teve sua primeira descrição em 2015, na China, em Escherichia coli portadora do gene mcr-1 (Mobile Colistin Resistance-1) e a partir de então tornou-se um grande desafio para a saúde pública em todo o mundo, constituindo uma grande ameaça à saúde humana e animal. Além disso, ainda existem poucos relatos sobre a prevalência de mcr-1 em Enterobacteriaceae isoladas de humanos, animais e alimentos. Sendo assim, o objetivo do estudo foi investigar a ocorrência do gene mcr-1 em isolados bacterianos com resistência fenotípica à polimixina B, oriundos de materiais clínicos de animais de companhia. A resistência fenotípica à polimixina B foi determinada por microdiluição em caldo e o perfil de sensibilidade aos demais antimicrobianos (amicacina, amoxicilina/clavulanato, ampicilina, ampicilina/sulbactam, aztreonam, cefazolina, cefepime, cefotaxima, cefoxitina, ceftazidima, ceftriaxona, cloranfenicol, ciprofloxacina, doxiciclina, ertapenem, gentamicina, imipinem, marbofloxacino, meropenem, fosfomicina, piperacilina/tazobactam, tetraciclina, ticarcilina/clavulanato, tobramicina sulfametoxazol/trimetoprim) foram determinados pelo método disco difusão. A extração do DNA bacteriano foi realizada via choque térmico, seguido de avaliação espectrofotométrica. Para a verificação da presença do mcr-1 foi utilizada a Reação em Cadeia da Polimerase com emprego de iniciadores específicos, seguida de eletroforese em gel de agarose. Os isolados positivos tiveram os correspondentes amplicons sequenciados. Nesse estudo foram identificados os primeiros isolados de Escherichia coli, Klebsiella spp. e Enterobacter spp. portadores do gene mcr-1 derivados de espécimes de animais de companhia no Brasil. Este estudo sugere a disseminação da resistência às polimixinas na comunidade e no meio ambiente, destacando a necessidade de vigilância e diretrizes otimizadas de tratamento.(AU)


Subject(s)
Animals , Dogs , Polymyxin B , Genes, MDR , Drug Resistance, Bacterial , Enterobacteriaceae , Cats
19.
Rev. bras. anal. clin ; 52(3): 255-259, 20200930. graf, tab
Article in Portuguese | LILACS | ID: biblio-1280799

ABSTRACT

As Enterobacteriaceae são bactérias Gram-negativas e frequentes causadoras de infecções hospitalares. Os carbapenêmicos (CROs) são considerados as mais recentes linhas de defesa contra infecções por microrganismos multirresistentes, no entanto o desenvolvimento de bactérias multirresistentes a esta classe medicamentosa tem prejudicado o tratamento farmacológico. Nesse sentido, o presente estudo trata-se de uma análise retrospectiva, tendo como objetivo avaliar o perfil de resistência e susceptibilidade de bacilos Gram-negativos fermentadores da glicose aos carbapenêmicos em isolados de hemoculturas positivas no período de janeiro de 2018 a janeiro 2019. Das 5.733 hemoculturas realizadas nesse período, cerca de 5,4% (311 amostras) foram positivas. Dentre essas, 34,4% (107 amostras) positivas para enterobactérias e 65,6% (204 amostras) positivas para outras espécies. Foi observada uma maior incidência de Klebsiella pneumoniae (34,6% dos casos), seguido pela Klebsiella sp. (28,9%) e a Escherichia coli (26,2%). Contudo, três (03) isolados de hemocultura da espécie Klebsiella pneumoniae se mostraram resistentes aos três antibióticos (ertapenem, meropenem e imipenem). Dois isolados de Enterobacter cloaceae também obtiveram resistência aos antibióticos utilizados e uma espécie de Enterobacter aerogenes mostrou-se resistente apenas ao ertapenem e meropenem e sensibilidade ao imipenem.Enterobacteriaceae are Gram-negative bacteria and frequent causes hospital infections. Carbapenemic (CRO) are considered the latest lines of defense against infections by multidrug-resistant microorganisms, however the development of multidrug-resistant bacteria in this drug class has impaired treatment pharmacological. The present study is a retrospective analysis, with the objective of evaluating the resistance and susceptibility profile from isolates in blood cultures from January 2018 to January 2019, from positive blood cultures with enterobacteria growth and analysis of antibiograms performed from fermenter Gram-negative glucose bacilli to carbapenem antimicrobials. Of the 5,733 blood cultures performed from January 2018 to January 2019, about 5.4% (311 samples) were positive. Among these, 34.4% (107 samples) positive for enterobacteria and 65.6% (204 samples) for other species. A higher incidence of Klebsiella pneumoniae (34.6% of cases) was observed, followed by Klebsiella sp. (28.9%) and Escherichia coli (26.2%), however, three hemoculture isolates of the species Klebsiella pneumoniae, which were resistant to the three antibiotics (ertapenem, meropenem and imipenem). Two (2) isolates of Enterobacter cloaceae also obtained resistance to the antibiotics used and one (1) species of Enterobacter aerogenes proved resistant only to ertapenem and meropenem and sensitivity to imipenem.


As Enterobacteriaceae são bactérias Gram-negativas e frequentes causadoras de infecções hospitalares. Os carbapenêmicos (CROs) são considerados as mais recentes linhas de defesa contra infecções por microrganismos multirresistentes, no entanto o desenvolvimento de bactérias multirresistentes a esta classe medicamentosa tem prejudicado o tratamento farmacológico. Nesse sentido, o presente estudo trata-se de uma análise retrospectiva, tendo como objetivo avaliar o perfil de resistência e susceptibilidade de bacilos Gram-negativos fermentadores da glicose aos carbapenêmicos em isolados de hemoculturas positivas no período de janeiro de 2018 a janeiro 2019. Das 5.733 hemoculturas realizadas nesse período, cerca de 5,4% (311 amostras) foram positivas. Dentre essas, 34,4% (107 amostras) positivas para enterobactérias e 65,6% (204 amostras) positivas para outras espécies. Foi observada uma maior incidência de Klebsiella pneumoniae (34,6% dos casos), seguido pela Klebsiella sp. (28,9%) e a Escherichia coli (26,2%). Contudo, três (03) isolados de hemocultura da espécie Klebsiella pneumoniae se mostraram resistentes aos três antibióticos (ertapenem, meropenem e imipenem). Dois isolados de Enterobacter cloaceae também obtiveram resistência aos antibióticos utilizados e uma espécie de Enterobacter aerogenes mostrou-se resistente apenas ao ertapenem e meropenem e sensibilidade ao imipenem.


Subject(s)
Carbapenems , Enterobacteriaceae
20.
Rev. bras. anal. clin ; 52(3): 235-237, 20200930. tab
Article in English | LILACS | ID: biblio-1255089

ABSTRACT

Objective: Characterize isolates of enterobacterias and pseudomonas based on their resistance to the antibiotics. Methods: This study was conducted with 44 samples from various species from the Enterobacteriaceae and Pseudomonaceae family and they were submitted to the Modified Hodge Test and Extended Spectrum Beta-Lactamase (ESBL) test. Results: From the 44 analyzed samples nine produced ESBL and ten produced some kind of carbapenemase. Conclusion: In total, 38,6% of the samples showed an increased in the incidence of this resistance in the last few years given that before 2001 there was not a single carbapenemase described. This fact alarms patients immunosuppressed or hospitalized since they colonize our digestive tract and can cause an infection given the opportunity.


Objetivo: Caracterizar isolados de enterobactérias e pseudomonas em relação a sua resistência aos antimicrobianos. Métodos: O estudo foi realizado com 44 amostras de várias espécies das famílias Enterobacteriaceae e Pseudomonaceae, as quais foram submetidas aos testes de Hodge Modificado e Beta-Lactamase de Espectro Estendido (ESBL). Resultados: Das 44 amostras analisadas nove são produtoras de ESBL e dez são produtoras de carbapenemase. Conclusão: No total, 38,6% das amostras apresentaram algum tipo de resistência às ESBL e carbapenemases, e esse número mostra um aumento na incidência dessas resistências nos últimos anos já que, antes de 2001, não haviam sido descritas as enzimas car­­­ba­­­penemases. Esse fato é alarmante para pacientes imunossuprimidos ou hospitalizados uma vez que as enterobactérias colonizam nosso trato digestivo e podem causar uma infecção se dada a oportunidade.


Subject(s)
Humans , Pseudomonas , beta-Lactam Resistance , Enterobacteriaceae , Carbapenem-Resistant Enterobacteriaceae
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