Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 462
Filter
1.
Article in Chinese | WPRIM | ID: wpr-828693

ABSTRACT

OBJECTIVE@#To study the clinical features of neonatal enterovirus infection, especially severe enterovirus infection.@*METHODS@#A retrospective analysis was performed for the clinical data of 244 neonates with enterovirus infection. According to the severity of infection, they were divided into a common infection group with 231 neonates and a severe infection group with 13 neonates. Clinical features were compared between the two groups.@*RESULTS@#Of the 244 neonates, 207 (84.8%) developed the disease in May to October, with the highest number of patients in June to July. Compared with the common infection group, the severe infection group had a significantly lower gestational age at birth and a significantly higher proportion of preterm infants (P<0.05). Compared with the common infection group, the severe infection group had a significantly earlier onset time (P<0.05) and significantly higher incidence rates of skin petechiae and ecchymosis, respiratory symptoms, sepsis-like manifestations (poor appetite, crying less, and less movement), concomitant diseases (such as pneumonia, myocarditis, necrotic hepatitis, and coagulation disorder), thrombocytopenia, prolonged prothrombin time, elevated creatine kinase-MB, and elevated alanine aminotransferase (P<0.05). The severe infection group had a significantly higher mortality rate than the common infection group (P<0.05).@*CONCLUSIONS@#There are significant differences in onset time, common clinical manifestations, and concomitant diseases between the neonates with common and severe enterovirus infection. In the enterovirus epidemic season, if the neonates have rashes and/or sepsis-like manifestations such as poor appetite and less movement, especially if the laboratory tests suggest liver damage and coagulation dysfunction, it is necessary to pay particular attention to the possibility of severe enterovirus infection.


Subject(s)
Enterovirus , Enterovirus Infections , Humans , Infant, Newborn , Infant, Premature , Retrospective Studies , Sepsis
2.
Arch. argent. pediatr ; 117(6): 664-669, dic. 2019. ilus
Article in Spanish | LILACS, BINACIS | ID: biblio-1046726

ABSTRACT

Desde la eliminación de la circulación del virus polio salvaje, disminuyeron los casos de parálisis fláccida aguda. Sin embargo, continúan ocurriendo casos asociados a otros enterovirus no polio y virus neurotropos. Se presenta el caso de una paciente de 9 años con diagnóstico de meningitis y mielitis con compromiso motor en los miembros inferiores y vejiga neurogénica asociado a enterovirus, con resolución completa del cuadro neurológico posterior a la administración de gammaglobulina hiperinmune.


Since the wild poliovirus no longer circulates, the number of cases of acute flaccid paralysis decreased. However, cases related to non-polio enteroviruses and neurotrope viruses continue to occur. We present a nine-year-old patient with meningitis and myelitis with motor involvement in the lower limbs and neurogenic bladder associated with enterovirus, with complete resolution of the neurological symptoms following the administration of hyperimmune gammaglobulin.


Subject(s)
Humans , Female , Child , gamma-Globulins/therapeutic use , Enterovirus , Myelitis/diagnostic imaging , Paralysis
3.
Rev. argent. microbiol ; 51(2): 140-143, jun. 2019. ilus, tab
Article in English | LILACS | ID: biblio-1013363

ABSTRACT

We present two groups of cases of atypical hand, foot, and mouth disease (HFMD) caused by Coxsackievirus A6 (CV-A6) detected in Argentina in 2015. The first group involved 14 patients from Chubut province and the second group affected 12 patients from San Luis province. Molecular analysis of the complete VP1 protein gene revealed the circulation of E2 sublineage, the most predominant worldwide. To our knowledge, this is the first report of CV-A6 infections associated with atypical HFMD in Argentina and South America.


Se describen dos grupos de casos de enfermedad de mano-pie-boca (HFMD) atípica causada por el virus Coxsackie A6 (Coxsackievirus A6, CV-A6) detectados en Argentina en el año 2015. El primero de los grupos involucró a 14 pacientes de Chubut y el segundo a 12 pacientes de San Luis. El análisis molecular del gen de la proteína VP1 completa reveló la circulación del sublinaje E2, el predominante a nivel global. Hasta donde sabemos, este es el primer reporte de infecciones CV-A6 asociadas con HFMD atípica en Argentina y Sudamérica.


Subject(s)
Enterovirus/pathogenicity , Hand, Foot and Mouth Disease/etiology , Hand, Foot and Mouth Disease/microbiology , Hand, Foot and Mouth Disease/epidemiology
4.
Bol. méd. postgrado ; 35(1): 25-30, Ene-Jun. 2019.
Article in Spanish | LILACS, LIVECS | ID: biblio-1120640

ABSTRACT

El Síndrome de Guillain Barré (SGB) se caracteriza por la manifestación de manera aguda o subaguda de un conjunto de signos y síntomas que demuestran la afectación del sistema nervioso periférico, expresada en parálisis fláccida y arreflexia, que eventualmente pueden complicarse amenazando la vida y/o posteriormente cronificarse si no se instauran tratamientos específicos de manera oportuna. Las causas más importantes del SGB se atribuyen a agentes infecciosos los cuales desencadenan un mecanismo de respuesta autoinmune que afectan tanto la mielina como el axón. La presente investigación caracterizó el SGB en los pacientes ingresados en el Servicio Desconcentrado Hospital Pediátrico Dr. Agustín Zubillaga entre los años 2010 y 2016(AU)


Guillain Barré Syndrome (GBS) is characterized by acute or subacute manifestation of peripheral nervous system alterations such as flaccid paralysis and arreflexia, which can eventually be life-threatening and/or become chronic if specific treatments are not established in a timely manner. The main causes of GBS are attributed to infectious agents which trigger an autoimmune response that affect both the myelin and the axon. GBS is the most frequent cause of flaccid paralysis in the pediatric population. The present investigation characterized the GBS in patients admitted to the Servicio Desconcentrado Hospital Pediátrico Dr. Agustín Zubillaga in the period 2010 to 2016(AU)


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Demyelinating Diseases , Peripheral Nervous System , Guillain-Barre Syndrome/physiopathology , Mycoplasma pneumoniae , Pediatrics , Enterovirus , Cytomegalovirus , Zika Virus
5.
Mem. Inst. Invest. Cienc. Salud (Impr.) ; 17(1): 25-31, abr. 2019. tab, ilus
Article in Spanish | LILACS, BDNPAR | ID: biblio-1007891

ABSTRACT

Aproximadamente 90% de las meningitis asépticas son causadas por enterovirus (EV), miembro de la familia de los picornavirus. Los EV son ubicuos, se diseminan por vía fecal-oral y contacto directo, responsables de brotes o casos esporádicos con importante morbilidad. El diagnóstico se basa en la presentación clínica, imágenes, estudio citoquímico del líquido cefalorraquídeo (LCR) y la Reacción en Cadena de la Polimerasa (PCR), gold estándard que reemplaza al aislamiento viral y la serología. El objetivo de este estudio descriptivo de corte transversal fue determinar la presencia de EV por RT-PCR en el LCR de pacientes con sospecha clínica de meningitis aséptica, internados en servicios públicos y privados de Asunción y departamento Central del Paraguay de noviembre de 2007 a noviembre de 2014. El material genético fue extraído mediante el mini kit ADN y ARN Qiagen® que fue sometido a RT-PCR. Se incluyeron LCR de 203 pacientes, 124 (61%) niños (4 días-15 años) y 79 (39%) adultos (16-81 años). Setenta y siete (38%) provenían de servicios públicos y 126 (62%) de privados; 115 (57%) fueron varones. Se detectó RNA de EV en 166 (82%) pacientes, 90 niños y 76 adultos, y mayor número de casos entre los meses de octubre a abril. Este es el primer trabajo en el país y muestra una importante participación del EV en pacientes por infecciones del SNC compatibles con meningitis asépticas de etiología viral. La sospecha clínica fue mayor en niños, sin embargo la proporción de resultados positivos fue mayor en adultos. Se observó mayor circulación en los meses cálidos(AU)


Subject(s)
Humans , Male , Female , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Enterovirus/genetics , Meningitis, Aseptic/virology , Paraguay , Polymerase Chain Reaction , Cross-Sectional Studies , Meningitis, Aseptic/diagnosis , Meningitis, Aseptic/cerebrospinal fluid
6.
Arch. argent. pediatr ; 117(1): 59-62, feb. 2019. ilus
Article in Spanish | LILACS, BINACIS | ID: biblio-983782

ABSTRACT

La enfermedad boca-mano-pie puede presentarse con formas atípicas, con lesiones más generalizadas y morfología diferente a la forma clásica. A veces, incluso simula otras enfermedades víricas. En las formas atípicas, existe la tendencia a afectar a las zonas de traumatismo o inflamación. Se presenta el caso de un niño de 2 años con antecedente de atopia, que consultó por presentar lesiones papulosas y vesiculosas umbilicadas que afectaban a la zona perioral, los miembros superiores e inferiores, con predominio en las zonas de presión y de dermatitis atópica previa. Aunque clínicamente se diagnosticó eczema herpético, las pruebas complementarias fueron negativas para herpes virus. Sin embargo, la reacción en cadena de la polimerasa del contenido de una vesícula, del exudado faríngeo y de heces fue positiva para enterovirus.


Hand-foot-mouth disease can present atypically, including forms with more numerous lesions and/or morphologically different from the classic presentation. It may even mimic other viral diseases. We present the case of a 2-year-old child previously diagnosed with atopic dermatitis, who presented with papules and umbilicated vesicles affecting the perioral area and limbs, predominantly in pressure areas, as well as in areas with previous atopic lesions. Although he was clinically diagnosed with herpetic eczema, tests results were negative for herpes virus. However, positive entorovirus polymerase chain reaction results were obtained from the content of a vesicle, a pharyngeal exudate and a stool sample.


Subject(s)
Humans , Male , Child, Preschool , Blister , Enterovirus , Exanthema , Hand, Foot and Mouth Disease
7.
Article in English | WPRIM | ID: wpr-760350

ABSTRACT

Enterovirus species G (EV-G) is highly diverse, and is ubiquitous in pig populations, usually without diarrhea. The present study aimed to investigate the presence of novel EV-G recombinants with the torovirus papain-like cysteine protease (PLCP) in Jeju pig herds. EV-G1-PLCP mono-infections were most prevalent in diarrheic weaned piglets. The PLCP genes of the Jeju isolates varied in size and junction sequences, and were greatly heterogeneous, with 77.0–90.7% homology amongst all recombinants. Our results suggest that the exogenous PLCP gene has undergone continuous rapid mutation in the individual EV-G genomes following cross-order recombination, thereby causing clinical disease in swine.


Subject(s)
Cysteine Proteases , Diarrhea , Enterovirus , Genome , Korea , Prevalence , Recombination, Genetic , Swine , Torovirus
8.
Article in English | WPRIM | ID: wpr-762284

ABSTRACT

BACKGROUND: Respiratory tract infections are major public health threats, and the identification of their causative microbes helps clinicians to initiate timely and appropriate antimicrobial therapy and prevent the secondary spread of infection. The main goal of this study was to compare two multiplex real-time polymerase chain reaction (PCR) assays used to detect respiratory viral pathogens in nasopharyngeal swab specimens. METHODS: Between September and October 2017, a total of 84 nasopharyngeal specimens were obtained consecutively from patients in a tertiary hospital using a flocked swab with 3 mL universal transport medium (COPAN Diagnostics, USA). A total of 64 positive and 20 negative sample results from the LG AdvanSure RV real-time RT-PCR kit (LG Life Sciences, Korea) were further retested using a new AdvanSure RV-plus a real-time RT-PCR kit to compare their performance. RESULTS: Statistical analysis of positive and negative agreement between the two different kits was conducted between the newly introduced AdvanSure RV-plus real-time RT-PCR kit and the AdvanSure RV real-time RT-PCR. The overall agreement was 96.4%, with positive agreement of 98.4% and negative agreement of 90%. The evaluated sensitivity and specificity of AdvanSure RV-plus real-time RT-PCR were 96.9% and 94.7%, respectively, with a kappa value of 0.9 (P<0.001). CONCLUSION: The performances of LG AdvanSure RV real-time RT-PCR and the new AdvanSure RV-plus real-time RT-PCR kit showed strong overall agreement. AdvanSure RV-plus real-time RT-PCR had a better detection rate and could detect coronavirus 229E and enterovirus, especially with a high detection rate in coinfection. AdvanSure RV-plus real-time RT-PCR can be considered a useful tool for respiratory virus diagnosis in clinical laboratories.


Subject(s)
Biological Science Disciplines , Coinfection , Coronavirus , Diagnosis , Enterovirus , Humans , Multiplex Polymerase Chain Reaction , Pneumonia , Public Health , Real-Time Polymerase Chain Reaction , Respiratory Tract Infections , Sensitivity and Specificity , Tertiary Care Centers
9.
Article in Korean | WPRIM | ID: wpr-719666

ABSTRACT

Viral respiratory infections are one of the most common infections worldwide. It is important to detect the virus early and precisely. In this study, we evaluated the limit of detection (LoD) and usefulness of the Real-Q RV Detection kit (BioSewoom, Seoul, Korea). We measured the LoD of the Real-Q RV Detection kit using 10 strains of standard viruses. We then compared the detection results by the Allplex Respiratory Panel Assay kit (Seegene, Seoul, Korea) using 123 clinical specimens. The discrepant results were confirmed by sequencing. Among the 10 standard viruses, the LoD of human rhinovirus (HRV) was the lowest and that of parainfluenza virus 2 and 3 was relatively high as detected by Real-Q RV Detection kit. Agreements of the two kits ranged from 95.9% to 100%. Three specimens detected negative by the Allplex Respiratory Panel kit were detected as adenovirus (AdV) by the Real-Q RV Detection kit and were confirmed by sequencing. Similarly, a specimen detected negative by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. A specimen detected as human enterovirus by the Allplex Respiratory Panel kit was detected as HRV by the Real-Q RV Detection kit and was confirmed by sequencing. Real-Q RV Detection kit showed good diagnostic performance and can be useful for detecting major viruses that cause respiratory infections.


Subject(s)
Adenoviridae , Enterovirus , Humans , Limit of Detection , Paramyxoviridae Infections , Respiratory Tract Infections , Rhinovirus , Seoul
10.
Article in English | WPRIM | ID: wpr-786526

ABSTRACT

Enteroviral infections are common in neonates. One important infection pathway is vertical transmission from an infected mother to her neonate. Here, we report the early detection and successful treatment of a vertically transmitted fulminant enteroviral infection associated with myocarditis and hepatitis. The patient had a sudden onset of high fever on the fourth day of life and developed severe, rapidly progressing symptoms of disseminated intravascular coagulopathy (DIC), hepatitis, and myocarditis accompanied by tachyarrhythmia. As it was the peak season for enteroviral infections and both the mother and the patient's 36-month-old sibling had a high fever around the time of delivery, we suspected an enteroviral infection. Thus, we initiated prompt evaluation of enteroviral infection, as well as close observation and intensive care of the neonate. We strongly recommend evaluation for the possibility of vertical enterovirus infection in neonates when the mother is suspected of having a viral infection (e.g., high fever and negative results from bacterial infectious studies) around the time of delivery and when the neonate shows some early symptoms of infectious diseases such as thrombocytopenia, DIC, hepatitis, and myocarditis. Early detection of enteroviral infections and prompt implementation of proper treatment are key to reduce the risk of complications and mortality associated with enteroviral infections in neonates.


Subject(s)
Arrhythmias, Cardiac , Child, Preschool , Communicable Diseases , Critical Care , Dacarbazine , Enterovirus , Enterovirus Infections , Fever , Hepatitis , Humans , Infant, Newborn , Mortality , Mothers , Myocarditis , Seasons , Siblings , Tachycardia , Thrombocytopenia
11.
Braz. j. microbiol ; 49(4): 790-794, Oct.-Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-974287

ABSTRACT

ABSTRACT Although the use of vaccines has controlled enteric diseases in dogs in many developed countries, vaccine coverage is still under optimal situation in Brazil. There is a large population of nonimmunized dogs and few studies about the identification of the viruses associated with diarrhea. To address this situation, stool samples from 325 dogs were analyzed by polymerase chain reaction for the detection of common enteric viruses such as Canine adenovirus (CAdV), Canine coronavirus (CCoV), Canine distemper virus (CDV), Canine rotavirus (CRV) and Carnivorous protoparvovirus 1 (canine parvovirus 2; CPV-2). At least one of these species was detected in 56.6% (184/325) of the samples. The viruses detected most frequently in either diarrheic or nondiarrheic dog feces were CPV-2 (54.3% of the positive samples), CDV (45.1%) and CCoV (30.4%), followed by CRV (8.2%) and CAdV (4.9%). Only one agent was detected in the majority of the positive samples (63%), but co-infections were present in 37% of the positive samples and mainly included CDV and CPV-2. The data presented herein can improve the clinical knowledge in regions with low vaccine coverage and highlight the need to improve the methods used to control these infectious diseases in domestic dogs.


Subject(s)
Animals , Dogs , Enterovirus/isolation & purification , Dog Diseases/virology , Enterovirus Infections/veterinary , Phylogeny , Brazil , Viral Vaccines/administration & dosage , Viral Vaccines/genetics , Viral Vaccines/immunology , Enterovirus/classification , Enterovirus/genetics , Dog Diseases/immunology , Dog Diseases/prevention & control , Enterovirus Infections/immunology , Enterovirus Infections/prevention & control , Enterovirus Infections/virology , Feces/virology
12.
Metro cienc ; 26(1): 12-15, jun. 2018.
Article in Spanish | LILACS | ID: biblio-981557

ABSTRACT

La encefalitis es una entidad rara con manifestaciones clínicas variables. En los niños es usualmente leve y autolimitada. En 30 a 75% se desconoce la etiología y cuando el patógeno se identifica por PCR (reacción en cadena de la polimerasa), el 80% son enterovirus. En América Latina no están disponibles métodos específicos de diagnóstico. En nuestro país muy pocos centros realizan el examen y tampoco están disponibles estadísticas reales de su incidencia. Objetivo: correlacionar la etiología con los síntomas, resultados de laboratorio, imagen y electroencefalograma. Métodos: estudio transversal; de enero a diciembre de 2016 se revisaron las historias clínicas de 63 niños de 0 a 18 años hospitalizados con sospecha de encefalitis. Una vez identificado el enterovirus por PCR, se correlacionó con los hallazgos de los exámenes de laboratorio e imagen. Resultados: se confirmó encefalitis viral en 35 pacientes, enterovirus en 49%, predominio en la edad preescolar. Síntomas más comunes: fiebre, cefalea, signos meníngeos y síntomas digestivos, 24% alteraciones de la conciencia. Biometría hemática normal en 71%, proteina C reactiva alterada en 71%. De los pacientes con estudios de imagen, 6% tuvo resultado anormal y en aquellos con electroencefalograma 80% fue anormal. Se utilizó la U de Mann Whitney para el análisis estadístico, la relación entre enterovirus y alteración de estado de conciencia y síntomas digestivos fue significativa (p=0.029). Conclusión: en los pacientes con diagnóstico de encefalitis que presenten síntomas digestivos y alteración del estado de conciencia, se debe sospechar que el enterovirus es el agente etiológico.


Encephalitis is a rare entity with variable clinical manifestations. In children it is usually mild and self-limited. In 30-75% of cases, the etiology is unknown and when the pathogen is identified by PCR (polymerase chain reaction), 80% are enteroviruses. In Latin America specific diagnostic methods are not available. In our country very few centers carry out the specific test, real statistics of their incidence are not available Objective: To correlate etiology with symptoms, laboratory results, imaging and electroencephalogram. Methods: Cross-sectional study, clinical records of 63 children between 0 and 18 years hospitalized with suspected encephalitis from January to December 2016 were reviewed. Once Enterovirus was identified by PCR, it was correlated with the findings of laboratory and imaging tests. Results: Viral encephalitis was confirmed in 35 patients, Enterovirus in 49%, predominance in pre-school age. Most common symptoms: fever, headache, meningeal signs and digestive symptoms, 24% alterations of consciousness. Normal blood count in 71%, Protein C Reactive altered in 71%. In patients with imaging studies, 6% had an abnormal result and in those who underwent electroencephalography, 80% were abnormal. The Mann Whitney U was used for the statistical analysis, the relationship between enterovirus and alteration of consciousness and digestive symptoms was significant (p: 0.029) Conclution: In patients with a diagnosis of encephalitis presenting digestive symptoms and altered state of consciousness, Enterovirus should be suspected as an etiologic agent.


Subject(s)
Humans , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Enterovirus , Encephalitis, Viral , Central Nervous System Viral Diseases , Encephalitis , Pediatrics , Signs and Symptoms, Digestive , Consciousness
13.
Arch. argent. pediatr ; 116(3): 192-197, jun. 2018. tab, graf
Article in English, Spanish | LILACS, BINACIS | ID: biblio-950009

ABSTRACT

Antecedentes. Las exacerbaciones de asma continúan siendo una causa de hospitalización en el Servicio de Urgencias. Los desencadenantesson alérgenos e infecciones, principalmente, de tipo viral. El objetivo fue determinar la relación entre los virus detectados durante la exacerbación asmática y los niveles de eosinófilos e inmunoglobulina E (IgE) sérica en pacientes pediátricos. Población y métodos. Estudio transversal analítico. Se incluyeron niños de cinco a quince años atendidos en Urgencias de Pediatría con exacerbación de asma, en el período de marzo de 2013 a febrero de 2016. Se obtuvo ácido ribonucleico viral en el aspirado nasofaríngeo con el kit CLART PneumoVir. Se cuantificaron los eosinófilos en la sangre periférica y los niveles de IgE sérica total. Se consideró eosinofilia un conteo ≥ 0,4 x 103/mm3 e IgE elevada, ≥ 350 UI/L. Se realizó la correlación de Pearson. Se definió significancia con valor de p ≤ 0,05.Resultados. De 211 niños con exacerbación de asma, en el 20%, se aisló un virus. Los virus aislados más frecuentemente fueron el rinovirus, el enterovirus y el virus sincitial respiratorio. Se encontró una correlación entre los niveles de eosinófilos e IgE sérica total en los niños con exacerbación de asma y rinovirus de 0,89, con una p= 0,0001.Conclusiones. Las infecciones por rinovirus, enterovirus y virus sincitial respiratorio son más frecuentes en las exacerbaciones de asma en menores de 15 años. Se observó una correlación entre los niveles de eosinófilos e IgE en presencia de rinovirus.


Background. Asthma exacerbations are still a cause of hospitalization at the Emergency Department. The triggers of asthma exacerbations include allergens and infections ­mostly viral­. The objective of this study was to establish the relationship between viruses detected during an asthma exacerbation and eosinophil and serum immunoglobulin E (IgE) levels in pediatric patients. Population and methods. Cross-sectional. analytical study. Children aged 5-15 years seen at the Pediatric Emergency Department with an asthma exacerbation in the period between March 2013 and February 2016 were included. Viral ribonucleic acid was extracted from nasopharyngeal aspirates using the CLART Pneumo Vir kit. Eosinophil levels were measured in peripheral blood and total IgE levels, in serum. Eosinophilia was defined as a count ≥ 0.4 x 103/mm3 and high IgE. as a level ≥ 350 IU/L. The Pearson's correlation was carried out. A value of p ≤ 0.05 was considered significant.Results. Out of 211 children with asthma exacerbation, a virus was isolated in 20%. The most commonly isolated viruses were rhinovirus. enterovirus, and respiratory syncytial virus. A correlation of 0.89 was established between eosinophil and total serum IgE levels in children with asthma exacerbation and rhinovirus, with a p value of 0.0001. Conclusions. Rhinovirus, enterovirus, and respiratory syncytial virus were the most common viruses in asthma exacerbations in children younger than 15 years. A correlation was established between eosinophil and IgE levels in the presence of rhinovirus.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Asthma/virology , Immunoglobulin E/blood , Eosinophils/metabolism , Asthma/physiopathology , Asthma/blood , Rhinovirus/isolation & purification , Cross-Sectional Studies , Respiratory Syncytial Virus, Human/isolation & purification , Respiratory Syncytial Virus Infections/diagnosis , Respiratory Syncytial Virus Infections/epidemiology , Enterovirus/isolation & purification , Picornaviridae Infections/diagnosis , Picornaviridae Infections/epidemiology , Emergency Service, Hospital , Enterovirus Infections/diagnosis , Enterovirus Infections/epidemiology
14.
Rev. Soc. Bras. Med. Trop ; 51(1): 30-38, Jan.-Feb. 2018. tab, graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS, CONASS, SES-RS | ID: biblio-897050

ABSTRACT

INTRODUCTION Infections caused by respiratory viruses are important problems worldwide, especially in children. Human metapneumovirus (hMPV) is a respiratory pathogen and causes severe infections with nonspecific symptoms. This study reports the hMPV occurrence and dissemination in southern Brazil and compares the frequency of occurrence of this virus and the human respiratory syncytial virus (hRSV) in the epidemiological weeks in a three-year period (2009-2011). METHODS: In total, 545 nasopharyngeal (NP) specimens from individuals with Severe Acute Respiratory Syndrome (SARS) who were negative for other seven respiratory viruses were analyzed for the presence of hMPV. Human metapneumovirus was detected by direct immunofluorescence and real-time reverse transcription polymerase chain reaction. RESULTS: hMPV was detected in 109 patients from the main geographic regions of the southernmost state of Brazil, presenting similar overall prevalence in males (46.8%) and females (53.2%). Among children who were less than six years old, hMPV was detected in 99 samples of all age groups, with a higher frequency in infants who were less than one year old (45.7%) compared to all other age groups until six years. hMPV and hRSV infection occurred in almost the same epidemiological weeks (EWs) of each year, with peaks of incidence between EW 31/37 and EW 26/38 for the years 2009 and 2011, respectively. hMPV was further detected in several cases of SARS and it was the only virus detected in three deaths. CONCLUSIONS These findings indicate that hMPV is in circulation in southern Brazil and highlight the importance of diagnosing hMPV for influenza-like illness in the population. (AU)


Subject(s)
Humans , Male , Female , Pregnancy , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Respiratory Tract Infections/transmission , Respiratory Tract Infections/virology , Respiratory Syncytial Virus Infections/virology , Metapneumovirus/pathogenicity , Epidemiological Monitoring , Adenoviruses, Human , Pneumovirinae/classification , Paramyxoviridae Infections/virology , Coronavirus , Enterovirus , Severe Acute Respiratory Syndrome , Influenza, Human , Human bocavirus
15.
Rev. Hosp. Niños B.Aires ; 60(269): 176-180, 2018.
Article in Spanish | LILACS | ID: biblio-1103283

ABSTRACT

El género Enterovirus contiene un gran número de agentes virales causantes de una amplia gama de enfermedades con diversas manifestaciones cutáneas, desde erupciones maculosas, maculopapulosas, urticariales, vesiculosas hasta petequiales. Los serotipos frecuentemente asociados con exantemas son Echovirus 9, 11, 16 y 25, los Virus Coxsackie A 2, 4, 9 y 16 y los Virus Coxackie B 3-5. Se presenta a continuación el caso de un paciente de 1 año y 9 meses con exantema máculo-eritematovesicular generalizado con base eritematosa pruriginoso, hiperalgesia cutánea y descamación posterior. Se detectó por reacción en cadena de polimerasa Enterovirus de lesión ampollar


The genus Enterovirus involve a large number of viral agents that cause a wide range of diseases, which are detected by cutaneous manifestations, such as macular, maculopapular, urticarial, vesiculous and petechial eruptions. The Serotypes frequently associated with exanthems are Echovirus 9, 11, 16 and 25 and Coxsackie viruses A 2, 4, 9 and 16 and Coxsackie B 3-5 viruses. The case of a 1-year and 9-month patient with generalized erythematous-vesicular bladder rash with pruritic erythematous base, cutaneous hyperalgesia and posterior desquamation is hereby presented. IT was detected by a polymerase chain reaction in a blister injury Enterovirus


Subject(s)
Male , Infant , Enterovirus
16.
Article in English | WPRIM | ID: wpr-758819

ABSTRACT

Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain reaction (PCR), which is based on TaqMan real-time PCR. Fifty-seven samples were positive for at least one pathogen, including bovine viral diarrhea virus, bovine enterovirus, Salmonella enterica ser. Dublin, Salmonella enterica ser. Typhimurium, and Neospora caninum; some samples were positive for multiple pathogens. Bovine viral diarrhea virus and bovine enterovirus were the most frequently detected pathogens, especially in flies, suggesting an important role of flies in the transmission of these viruses. Additionally, we detected the N. caninum genome from a cockroach sample for the first time. Our data suggest that insects (particularly flies), birds, and rodents are potential vectors and reservoirs of abortion, diarrhea, and respiratory infectious agents, and that they may transmit more than one pathogen at the same time.


Subject(s)
Animals , Birds , Cattle , Cockroaches , Diarrhea Viruses, Bovine Viral , Diarrhea , Diptera , Disease Reservoirs , Disease Vectors , Enterovirus , Enterovirus, Bovine , Genome , Insecta , Neospora , Polymerase Chain Reaction , Real-Time Polymerase Chain Reaction , Rodentia , Salmonella enterica , Virulence Factors
17.
Article in English | WPRIM | ID: wpr-776071

ABSTRACT

OBJECTIVE@#Newly identified human rhinovirus C (HRV-C) and human bocavirus (HBoV) cannot propagate in vitro in traditional cell culture models; thus obtaining knowledge about these viruses and developing related vaccines are difficult. Therefore, it is necessary to develop a novel platform for the propagation of these types of viruses.@*METHODS@#A platform for culturing human airway epithelia in a three-dimensional (3D) pattern using Matrigel as scaffold was developed. The features of 3D culture were identified by immunochemical staining and transmission electron microscopy. Nucleic acid levels of HRV-C and HBoV in 3D cells at designated time points were quantitated by real-time polymerase chain reaction (PCR). Levels of cytokines, whose secretion was induced by the viruses, were measured by ELISA.@*RESULTS@#Properties of bronchial-like tissues, such as the expression of biomarkers CK5, ZO-1, and PCK, and the development of cilium-like protuberances indicative of the human respiration tract, were observed in 3D-cultured human airway epithelial (HAE) cultures, but not in monolayer-cultured cells. Nucleic acid levels of HRV-C and HBoV and levels of virus-induced cytokines were also measured using the 3D culture system.@*CONCLUSION@#Our data provide a preliminary indication that the 3D culture model of primary epithelia using a Matrigel scaffold in vitro can be used to propagate HRV-C and HBoV.


Subject(s)
Collagen , Drug Combinations , Enterovirus , Enterovirus Infections , Virology , Enzyme-Linked Immunosorbent Assay , Epithelial Cells , Virology , Human bocavirus , Humans , Laminin , Parvoviridae Infections , Virology , Primary Cell Culture , Methods , Proteoglycans , Real-Time Polymerase Chain Reaction , Respiratory Mucosa , Virology , Virus Cultivation
18.
Article in English | WPRIM | ID: wpr-715376

ABSTRACT

Epidemic keratoconjunctivitis (EKC) and acute hemorrhagic conjunctivitis (AHC) are common diseases caused by human adenoviruses (HAdV) and enteroviruses, respectively, in South Korea. However, there are limited studies on the molecular epidemiology of viral conjunctivitis in South Korea. The main objective of this study was to characterize the genotypes of adenoviruses and enteroviruses causing viral conjunctivitis in the southwest region of South Korea. We collected conjunctival swabs from 492 patients with suspected cases of viral conjunctivitis from 6 ophthalmic hospitals in Gwangju Metropolitan City, in South Korea, between 2012 and 2016. Of the 492 samples tested, HAdVs and enteroviruses were detected in 249 samples (50.6%) and 19 samples (3.9%), respectively. The genotype analysis detected HAdV-8 in 183 samples (73.5%), HAdV-37 in 14 samples (5.6%), and HAdV-3, and HAdV-4 in 9 samples (3.6%) each. We detected coxsackievirus A24 (CVA24) and coxsackievirus B1 (CVB1) in 8 samples (42.0%) and 4 samples (21.0%), respectively. We also reported for the first time HAdV-56-infected cases of EKC in South Korea. Furthermore, we found three cases of coinfection with HAdV and enterovirus genotypes in our samples. HAdV-8 and CVA24, the main causes of EKC and AHC, respectively, worldwide, were also found to be the predominant genotypes in our study.


Subject(s)
Adenoviridae , Adenoviruses, Human , Coinfection , Conjunctivitis, Acute Hemorrhagic , Conjunctivitis, Viral , Enterovirus , Genotype , Humans , Keratoconjunctivitis , Korea , Molecular Epidemiology
19.
Article in English | WPRIM | ID: wpr-728847

ABSTRACT

PURPOSE: Despite the fact that enteroviral meningitis is the most common cause of meningitis in children with signs of meningeal irritation and has benign course, most clinicians routinely perform an invasive lumbar puncture (LP) that result in inadequate antibiotic therapy and unnecessary long-term hospitalization. This study was aimed to compare the clinical characteristics of childhood enteroviral meningitis according to LP. METHODS: Children over 2 years of age who can clearly express signs of meningeal irritation in the pediatric department of Eulji university hospital from July 2013 and August 2016 were enrolled. The patients were diagnosed by reverse transcriptase polymerase chain reaction in stool and/or cerebrospinal fluid. We retrospectively reviewed their clinical records. RESULTS: A total of 257 patients were diagnosed with enterovirus meningitis and median age was 6.3 (2.1–7.9) years. One hundred fifteen patients (44.7%) with confirmed enterovirus in the stools underwent supportive care without LP. Mostly, there was no statistically significant difference in age, sex, clinical symptoms, except gastrointestinal involvement (abdominal pain, diarrhea), and serologic findings when compared with patients who underwent LP. But patients who underwent LP had longer hospital stay (4 vs 3 days, P < 0.001). Four of them (2.8%) were re-admitted with back pain and persistent headache, probably related to LP procedure. All patients were discharged without neurologic complications. CONCLUSION: Rapid detection of enteroviruses in stool specimens that can be easily obtained in children with signs of meningeal irritation may reduce invasive LP.


Subject(s)
Back Pain , Cerebrospinal Fluid , Child , Enterovirus , Headache , Hospitalization , Humans , Length of Stay , Meningitis , Retrospective Studies , Reverse Transcriptase Polymerase Chain Reaction , Spinal Puncture
20.
Article in Korean | WPRIM | ID: wpr-728834

ABSTRACT

PURPOSE: Acute encephalitis and encephalopathy are preceded by respiratory or enteric infection, whose pathogens can be detected more easily with advanced tools. However, studies for pathogens in Korea remain scarce. We investigated the clinical characteristics and pathogens in childhood encephalitis and encephalopathy. METHODS: We retrospectively reviewed the records of children with acute encephalitis and encephalopathy admitted to our hospital between March 2013 and February 2017. RESULTS: The 51 included patients were aged 5.8±4.4 years (mean±standard deviation), comprising 36 with encephalitis (70.6%) and 15 with encephalopathy (29.4%). Respiratory symptoms (62.7%) were more common than enteric symptoms (45.1%). Brain MRI was abnormal in 54.9%, and leu-kocytosis in the cerebrospinal fluid was noted in 41.2%. The prevalence of diseases was highest in winter (29.4%). In encephalitis, eight patients had infective encephalitis (15.7%), comprising enterovirus (N=4), Epstein-Barr virus (N=3; one with HHV6 coinfection), and tsutsugamushi in-fection (N=1). The 11 patients with ADEM included 1each with adenovirus, influenza A, and mycoplasmal infection. One patient with Bickerstaff-brainstem encephalitis had mycoplasmal pneumonia. In the 15 patients with encephalitis of unknown etiology, rhinovirus (N=3), influenza A (N=2), adenovirus (N=1), and mycoplasmal infection (N=6) were found. In the encephalopa-thy group, three patients had abnormal brain MRI: ANE with influenza A, AESD with exanthem subitum, and norovirus-associated MERS. In the remaining 12 patients, influenza A (N=2), ade-novirus, rhinovirus, enterovirus, norovirus (N=1 for each virus), and mycoplasmal infection (N=4) were found. CONCLUSION: Acute childhood encephalitis and encephalopathy were the most prevalent in winter and were fre-quently associated with respiratory infections.


Subject(s)
Adenoviridae , Bacteria , Brain , Brain Diseases , Cerebrospinal Fluid , Child , Encephalitis , Enterovirus , Exanthema , Herpesvirus 4, Human , Herpesvirus 6, Human , Humans , Influenza, Human , Korea , Magnetic Resonance Imaging , Norovirus , Pneumonia , Prevalence , Respiratory Tract Infections , Retrospective Studies , Rhinovirus
SELECTION OF CITATIONS
SEARCH DETAIL