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Arq. bras. med. vet. zootec. (Online) ; 72(3): 926-930, May-June, 2020. ilus, tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1129601


Papillomatosis is one of the few diseases in which autovaccine therapy is recommended, especially in the case of mass manifestation in animals. It is noted that papillomas are diagnosed in 15.7% of cases in histological diagnosis of oncological diseases. Therefore, this work studied the profile of oncological diseases in dogs and the clinical effectiveness of autovaccine therapy of papillomatosis. The article gives a morphological description of papillomas used as a biomaterial, and also describes the technological methods of manufacturing an autovaccine used to treat animals against papillomatosis. A therapeutic scheme for administering the vaccine was proposed and tested, and the results of the clinical trial are presented, as well as a reflection on some moments of the mechanism of vaccine therapy for papillomatosis.(AU)

A papilomatose é uma das poucas doenças onde se recomenda a terapia com autovacina, principalmente no caso de manifestação em massa em animais. Nota-se que papilomas são diagnosticados em 15,7% dos casos em diagnóstico histológico de doenças oncológicas. Portanto, este trabalho estudou o perfil das doenças oncológicas em cães e a eficácia clínica da terapia com autovacina em papilomatose. O presente artigo fornece descrição morfológica dos papilomas utilizados como biomaterial, além da descrição dos métodos tecnológicos de fabricação de uma autovacina usada para tratar animais contra a papilomatose. Um esquema terapêutico para a administração da vacina foi proposto e testado, e os resultados do ensaio clínico são apresentados, e é feita uma reflexão sobre alguns momentos do mecanismo da terapia de vacina para papilomatose.(AU)

Animals , Dogs , Papilloma/veterinary , Papilloma/epidemiology , Epithelial Cells/pathology , Autovaccines/therapeutic use , Immunotherapy/veterinary
Int. j. odontostomatol. (Print) ; 13(3): 332-337, set. 2019. graf
Article in Spanish | LILACS | ID: biblio-1012432


RESUMEN: Este estudio se realizó para evaluar la presentación de cambios histopatológicos tempranos en sacos pericoronarios de terceros molares incluidos sanos. Sesenta y un tejidos pericoronarios de terceros molares incluidos, clínicamente asintomáticos y sin evidencia radiográfica de anomalía del saco pericoronario, se extrajeron por diferentes razones. Las muestras se fijaron y procesaron de forma rutinaria y se tiñeron con hematoxilina-eosina antes de la evaluación. Se estandarizaron los diagnósticos concluyendo que toda muestra que presente metaplasia escamosa del epitelio reducido del órgano del esmalte (EEE) se considerará cambio quístico temprano. De las muestras presentadas, el 28 % mostró metaplasia escamosa sugerente de cambio quístico temprano. Esta variación histológica del tejido pericoronario fue significativamente mayor en los pacientes menores de 20 años de edad, sexo femenino, terceros molares mandibulares, en inclusión ósea y en pacientes que no informaron sintomatología local previa (P = 0,05). Estos hallazgos sugieren que la ausencia de semiología clínica y radiográfica en terceros molares incluidos no necesariamente indica ausencia de alteraciones en el tejido pericoronario de los mismos.

ABSTRACT: This study was performed to evaluate the presentation of early histopathological changes in pericoronal healthy third molars. Sixty-one pericororonitis tissues third molars, without radiographic evidence of abnormality in pericoronary sack, extracted for various reasons. The samples were fixed and processed routinely and stained with hematoxylin-eosin before evaluation. Diagnostic standardized concluding that all this shows that squamous metaplasia of the reduced enamel organ (EEE) is considered cystic epithelium were early change. Of the samples submitted, 28 % showed squamous metaplasia early suggestive of cystic change. This histological tissue pericoronary variation was significantly higher in patients under 20 years of age, female gender, mandibular third molars, including in bone and in patients who reported no previous local symptoms (P = 0.05). These findings suggest that the absence of clinical and radiographic semiology third molars does not necessarily indicate the absence of alterations in tissue pericoronary thereof.

Humans , Male , Female , Child , Adolescent , Adult , Tooth Eruption , Tooth, Unerupted , Gingival Diseases/pathology , Hamartoma/pathology , Biopsy , Chile , Epidemiology, Descriptive , Connective Tissue/pathology , Diagnosis , Epithelial Cells/pathology , Informed Consent
Acta cir. bras ; 34(6): e201900602, 2019. tab, graf
Article in English | LILACS | ID: biblio-1019265


Abstract Purpose To investigate the role and related mechanisms of miR-106a in sepsis-induced AKI. Methods Serum from sepsis and healthy patients was collected, sepsis mouse model was established by cecal ligation and puncture (CLP). TCMK-1 cells were treated with lipopolysaccharide (LPS) and transfected with THBS2-small interfering RNA (siTHBS2), miR-106a inhibitor, miR-106a mimics and their negative controls (NCs). The expression of miR-106a, thrombospondin 2 (THBS2), Bax, cleaved caspase-3 and Bcl-2, cell viability, relative caspase-3 activity and TNF-α, IL-1β, IL-6 content were respectively detected by quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, Cell Counting Kit-8 (CCK-8) and enzyme linked immunosorbent assay (ELISA). The relationship between miR-106a and THBS2 was confirmed by dual luciferase reporter assay. Results MiR-106a was up-regulated in serum of sepsis patients, CLP-induced mice models and LPS-induced TCMK-1 cells. LPS reduced cell viability and Bcl-2 expression, and increased caspase-3 activity, Bax expression, the content of TNF-α, IL-1β, IL-6. THBS2 was a target of miR-106a. The decreases of caspase-3 activity, TNF-α, IL-1β, IL-6, Bax expression and the increases of cell viability, Bcl-2 expression caused by miR-106a knockdown were reversed when THBS2 silencing in LPS-stimulated TCMK-1 cells. Conclusion MiR-106a aggravated LPS-induced inflammation and apoptosis of TCMK-1 cells via regulating THBS2 expression.

Humans , Animals , Male , Female , Adult , Middle Aged , Rats , Sepsis/pathology , Thrombospondins/pharmacology , MicroRNAs/metabolism , Epithelial Cells/pathology , Acute Kidney Injury/metabolism , Kidney/cytology , Enzyme-Linked Immunosorbent Assay , Transfection , Case-Control Studies , Cells, Cultured , Cytokines/metabolism , Apoptosis , Sepsis/metabolism , Disease Models, Animal , Acute Kidney Injury/pathology , Real-Time Polymerase Chain Reaction
Rev. bras. cir. plást ; 33(3): 382-388, jul.-set. 2018. ilus
Article in English, Portuguese | LILACS | ID: biblio-965592


Introdução: O ceratoacantoma é uma neoplasia epitelial de rápido crescimento, mais frequente em áreas de exposição solar. Habitualmente, apresenta-se como lesão única, arredondada, com depressão central preenchida de queratina. As semelhanças clínicas e histopatológicas com o carcinoma de células escamosas, frequentemente, dificultam o diagnóstico diferencial. A biópsia excisional é a abordagem de escolha, permitindo diagnóstico e tratamento. Método: O presente estudo é observacional e retrospectivo, com dados de 162 pacientes tratados de 2005 a 2013, no Hospital Felício Rocho, em Belo Horizonte, MG. Todos os pacientes submeteram-se à excisão cirúrgica dos tumores. Foram estudados: sexo, idade, número de lesões, localização, tamanho do tumor e diagnóstico pré-operatório. Resultados: Dos 162 pacientes, totalizando 173 lesões, 154 (95,06%) apresentavam ceratoacantoma único. Noventa e dois eram do gênero masculino (56,80%) e 70 do feminino (43,20%). A idade dos pacientes variou de 11 a 96 anos, com média de 71,23 anos. As lesões localizavam-se predominantemente nos membros superiores (43,64%), na face (28,48%) e nos membros inferiores (17,58%). Nas hipóteses diagnósticas formuladas pelos cirurgiões, no pedido do exame anatomopatológico, houve diagnóstico correto em 63,13%. Conclusão: O ceratoacantoma é uma neoplasia epitelial de características morfológicas semelhantes ao carcinoma de células escamosas, o que, por muitas vezes, dificulta o diagnóstico. Torna-se necessária, portanto, a excisão cirúrgica completa das lesões suspeitas para diagnóstico e tratamento corretos.

Introduction: Keratoacanthoma is an epithelial neoplasm of rapid growth, more frequent in areas of sun exposure, and usually appears as a single, rounded lesion with a central depression filled with keratin. Clinical and histopathological similarities with squamous cell carcinoma often make differential diagnosis difficult. Excisional biopsy is the approach of choice, allowing diagnosis and treatment. Method: This is an observational and retrospective study, in which data of 162 patients treated at the Hospital Felício Rocho from 2005 to 2013, in Belo Horizonte, MG, were analyzed. All patients underwent surgical excision of tumors. Data on sex, age, number of lesions, location, tumor size, and preoperative diagnosis were studied. Results: Of the 162 patients, with a total of 173 lesions, only 154 (95.06%) had keratoacanthoma. There were 92 male (56.80%) and 70 female (43.20%) patients. The age of patients ranged from 11 to 96 years, with an average of 71.23 years. The lesions were located predominantly in the upper limbs (43.64%), face (28.48%), and lower limbs (17.58%). In the diagnostic hypotheses formulated by surgeons at the request of the pathology, the diagnosis was correct in 63.13%. Conclusion: Keratoacanthoma is an epithelial tumor with morphological characteristics similar to those of squamous cell carcinoma, which often complicates the diagnosis. Therefore, the complete excision of the suspicious lesions is necessary for correct diagnosis and treatment.

Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Skin Neoplasms/surgery , Surgical Procedures, Operative/methods , Biopsy/methods , Reconstructive Surgical Procedures/methods , Epithelial Cells/pathology , Epithelium/surgery , Intraoperative Complications/surgery , Keratoacanthoma/surgery , Keratoacanthoma/pathology , Cell Proliferation , Keratoacanthoma
Rev. Assoc. Med. Bras. (1992) ; 64(3): 214-216, Mar. 2018. graf
Article in English | LILACS | ID: biblio-896451


Summary An 82-year-old man sought our service with dysphagia and was referred for upper endoscopy with biopsies, which evidenced multiple ulcers of the esophagus and oropharinx. Histopathology confirmed the unusual diagnosis of esophageal lichen planus. The correct clinical suspicion of this disease can facilitate the diagnosis and guide specific treatment, which can drastically change the natural course of the disease.

Resumo Paciente do sexo masculino, de 82 anos, com disfagia, foi encaminhado para realização de endoscopia digestiva alta com biópsias, na qual foram evidenciadas múltiplas úlceras de esôfago e orofaringe. O estudo histopatológico confirmou o diagnóstico raro de líquen plano esofágico. A correta suspeita clínica dessa doença pode facilitar o diagnóstico e direcionar para um tratamento específico, o que pode drasticamente alterar o curso natural dessa comorbidade.

Humans , Male , Aged, 80 and over , Deglutition Disorders/etiology , Deglutition Disorders/diagnostic imaging , Esophageal Diseases/complications , Lichen Planus/complications , Biopsy , T-Lymphocytes/pathology , Esophagoscopy , Epithelial Cells/pathology , Esophageal Diseases/pathology , Esophageal Diseases/diagnostic imaging , Lichen Planus/pathology , Lichen Planus/diagnostic imaging
An. bras. dermatol ; 93(2): 256-258, Mar.-Apr. 2018. graf
Article in English | LILACS | ID: biblio-887184


Abstract: Primary cutaneous lymphoepithelioma-like carcinoma is a rare disease with low metastatic potential. Its morphologic and pathological features are similar to those of nasopharyngeal lymphoepithelial carcinoma. We report the case of a 60-year-old man with an infrapalpebral pearly papule, measuring 0.6 cm in diameter. The lesion was excised with a clinical hypothesis of basal cell carcinoma or squamous cell carcinoma. Histopathological analysis revealed a malignant neoplasm with syncytial arrangement of cells with vesicular nuclei, associated with dense lymphocytic infiltrate. Immunohistochemistry revealed cytokeratin-positive cells (AE1/AE3) and p63 protein, indicating epithelial histogenesis and squamous differentiation. A negative Epstein-Barr virus test result was achieved by immunohistochemistry. Primary lymphoepithelioma-like carcinoma of the skin is a differential diagnosis of lesions with prominent inflammatory infiltrates.

Humans , Male , Middle Aged , Skin Neoplasms/pathology , Carcinoma/pathology , Biopsy , Immunohistochemistry , Carcinoma, Basal Cell/pathology , Carcinoma, Squamous Cell/pathology , Diagnosis, Differential , Epithelial Cells/pathology
Int. j. morphol ; 35(4): 1576-1581, Dec. 2017. tab, graf
Article in Spanish | LILACS | ID: biblio-893171


RESUMEN: El objetivo de este estudio fue valuar la utilidad del uso de la tinción de Tricrómico de Masson (TM) en la cuantificación de la densidad media vascular (DMV) en Mucosa Oral Normal (MON), Displasia Epitelial Oral (DEO) y Carcinoma Oral de Células Escamosas (COCE). Estudio descriptivo de serie de casos. Se analizaron 17 muestras de MON, 15 muestras de DEO y 16 de COCE, teñidas con TM. Para determinar su utilidad, se compararon con las mismas muestras analizadas con técnica de inmunohistoquímica contra CD31. La cuantificación de la DMV se realizó en las 3 áreas de mayor vascularización de cada muestra. Se determinó la DMV según diagnóstico mediante la tinción TM e inmunohistoquímica contra CD31, y se calculó la correlación entre ambos. La DMV cuantificada con TM y contra CD31 difiere según el diagnóstico, observándose un aumento de la DMV al malignizarse el diagnóstico. No se encontraron diferencias al comparar la DMV cuantificada con TM y contra CD31. La correlación de la DMV analizado por TM y contra CD31 es significativa y moderada. La cuantificación de vasos sanguíneos es posible mediante la tinción de TM en muestras de MON, DEO y COCE, con una correlación moderada con la inmunohistoquímica contra CD31.

SUMMARY. The objective of this study was to evaluate the utility of Masson's Trichrome (TM) staining in the quantification of the mean vascular density (DMV) in samples of normal oral mucosa (MON), oral epithelial dysplasia (ODE) and oral squamous cell carcinoma (COCE). The design - a descriptive study of case series. We analyzed 17 samples of MON, 15 samples of DEO and 16 samples of COCE, stained with TM. To determine usefulness, we compared and analyzed the same samples, either stained with TM or with immunohistochemical technique against CD31. Quantification of the DMV was performed in the 3 areas of greatest vascularization in each sample. DMV was determined according to diagnosis by TM staining and immunohistochemistry against CD31, and the correlation between the two was then calculated. DMV quantified with TM and against CD31 differs according to the diagnosis, with an increase in DMV upon malignant diagnosis. No differences were found when comparing DMV quantified with TM and against CD31. The correlation of the DMV analyzed by TM and against CD31 is significant and moderate. Quantification of blood vessels is possible by TM staining in samples of MON, DEO and COCE. TM staining is moderately correlated with immunohistochemistry against CD31.

Humans , Male , Female , Adult , Middle Aged , Aged , Carcinoma, Squamous Cell/pathology , Mouth Neoplasms/pathology , Neovascularization, Pathologic/pathology , Staining and Labeling/methods , Epithelial Cells/pathology , Immunohistochemistry , Mouth Mucosa/pathology , Platelet Endothelial Cell Adhesion Molecule-1
Rev. otorrinolaringol. cir. cabeza cuello ; 77(3): 295-299, set. 2017. graf
Article in Spanish | LILACS | ID: biblio-902779


Se presenta caso de paciente de sexo femenino de 53 años de edad con tumor parotídeo izquierdo de larvada evolución, con crecimiento progresivo y otalgia ipsilateral en los últimos meses, estudiado previamente con tomografía de cuello con contraste y resonancia magnética que destacan masa del lóbulo profundo de la parótida de características imagenológicas benignas. Se realizó parotidectomía del lóbulo profundo con resección tumoral preservando el nervio facial casi en su totalidad con excepción de rama marginal, la biopsia de la pieza quirúrgica fue informada como carcinoma epitelialmioepitelial de bajo grado, un tumor infrecuente de las glándulas salivales. Se decidió completar la parotidectomía superficial y realizar vaciamiento ganglionar selectivo lateral ipsilateral, complementando el tratamiento con radioterapia. Además se presenta una revisión de la literatura correspondiente.

We present a case of a 53 years old female patient with a left parotid tumor, with slow evolution, progressive growth and ipsilateral otalgia during later months. She was previously studied by tomography of the neck with contrast and magnetic resonance, which showed the mass of the deep lobe to have benign imaging characteristics. A parotidectomy of deep lobe was performed, with tumoral resection, preserving the facial nerve with the exception of the marginal branch. The biopsy was informed as epithelial-myoephitelial carcinoma, a rare salivary gland tumor. We completed the parotidectomy with neck dissection and Radiotherapy complementary was made. Besides we presented a literature review.

Humans , Female , Middle Aged , Parotid Neoplasms/surgery , Myoepithelioma/surgery , Parotid Neoplasms/pathology , Parotid Neoplasms/diagnostic imaging , Magnetic Resonance Imaging , Myoepithelioma/pathology , Myoepithelioma/diagnostic imaging , Epithelial Cells/pathology
Braz. oral res. (Online) ; 31: e34, 2017. tab, graf
Article in English | LILACS | ID: biblio-839511


Abstract The aim of this study was to evaluate the immunoexpression of glucose transporters 1 (GLUT-1) and 3 (GLUT-3) in keratocystic odontogenic tumors associated with Gorlin syndrome (SKOTs) and non-syndromic keratocystic odontogenic tumors (NSKOTs), and to establish correlations with the angiogenic index. Seventeen primary NSKOTs, seven recurrent NSKOTs, and 17 SKOTs were selected for the study. The percentage of immunopositive cells for GLUT-1 and GLUT-3 in the epithelial component of the tumors was assessed. The angiogenic index was determined by microvessel count. The results were analyzed statistically using the nonparametric Kruskal-Wallis test and Spearman’s correlation test. High epithelial immunoexpression of GLUT-1 was observed in most tumors (p = 0.360). There was a higher frequency of negative cases for GLUT-3 in all groups. The few GLUT-3-positive tumors exhibited low expression of this protein in epithelial cells. No significant difference in the angiogenic index was observed between groups (p = 0.778). GLUT-1 expression did not correlate significantly with the angiogenic index (p > 0.05). The results suggest that the more aggressive biological behavior of SKOTs when compared to NSKOTs may not be related to GLUT-1 or GLUT-3 expression. GLUT-1 may play an important role in glucose uptake by epithelial cells of KOTs and this process is unlikely related to the angiogenic index. GLUT-1 could be a potential target for future development of therapeutic strategies for KOTs.

Humans , Basal Cell Nevus Syndrome/pathology , Glucose Transporter Type 1/analysis , Glucose Transporter Type 3/analysis , Neovascularization, Pathologic/pathology , Odontogenic Cysts/pathology , Odontogenic Tumors/pathology , Basal Cell Nevus Syndrome/metabolism , Epithelial Cells/pathology , Immunohistochemistry , Odontogenic Cysts/chemistry , Odontogenic Tumors/chemistry , Paraffin Embedding , Reference Values , Statistics, Nonparametric
Braz. oral res. (Online) ; 31: e51, 2017. tab, graf
Article in English | LILACS | ID: biblio-952089


Abstract The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 periapical granulomas and 22 radicular cysts) were subjected to morphological and immunohistochemical analyses using anti-MMP-9 and anti-TGF-β1 antibodies. The data were analyzed using the following tests: non-parametric Mann-Whitney, chi-square, Fisher's exact test and Spearman's correlation test (P<0.05). Analysis of inflammatory infiltrate revealed that 78% of periapical granulomas presented infiltrate grade III, in contrast with 32% of radicular cysts (P<0.001). Morphological evaluation of the epithelial thickness in radicular cysts revealed the presence of atrophic epithelium in 86% of the cysts. The immunostaining of MMP-9 was score 2 in 67% of the granulomas and 77% of the cysts. Both lesions were predominantly score 1 for TGF-β1. Significant differences were confirmed between the expression scores of TGF-β1 and MMP-9 in periapical granulomas (p = 0.004) and in radicular cysts (p < 0.001). Expression of TGF-β1 was different for periapical granulomas and radicular cysts. This immunoregulatory cytokine seems more representative in asymptomatic lesions. The extracellular matrix remodeling process dependent on MMP-9 seems to be similar for both periapical granulomas and radicular cysts. TGF-β1 and MMP-9 may play an important role in the maintenance of periapical lesions.

Humans , Male , Female , Adult , Periapical Granuloma/metabolism , Radicular Cyst/chemistry , Matrix Metalloproteinase 9/analysis , Transforming Growth Factor beta1/analysis , Periapical Granuloma/immunology , Periapical Granuloma/pathology , Biopsy , Severity of Illness Index , Immunohistochemistry/methods , Radicular Cyst/immunology , Radicular Cyst/pathology , Statistics, Nonparametric , Epithelial Cells/pathology
Int. j. odontostomatol. (Print) ; 10(1): 113-118, abr. 2016. ilus
Article in English | LILACS | ID: lil-782630


The objective of this study was to determine the distribution of epithelial odontogenic tumors diagnosed histologically in a period of 41 years in a Brazilian population according to age, gender, site affected and compare these data with previously reported studies from other countries. Data of epithelial odontogenic tumors diagnosed were collected from the files of the Oral Pathology Laboratory of Federal University of Rio Grande do Norte, Natal, RN, Brazil, and entered in a standardized form for later comparisons. Clini-cal features obtained from the patient records and microscope slides were reviewed according to the 1992 World Health Organization classification. A total 156 epithelial odontogenic tumor were reported. Of these, all of them were benign. Ameloblastoma was the most frequent type (85.9 %), followed by adenomatoid odontogenic tumor (10.9 %) and calcifying epithelial odontogenic tumor (3.2 %). The mean age of the patients was 38 years, with a wide range (11­80 years). The posterior region of mandible was the anatomic site most frequently affected by this disease, and no significant differences were found between sexes in the diagnosis of odontogenic tumors. A marked geographic variation in the relative incidences of various epithelial odontogenic tumors was found. It was particularly notable in ameloblastomas and adenomatoid odontogenic tumors, with the incidences observed in the present study being similar, sometimes different to earlier studies in others parts of the world.

El objetivo fue determinar la distribución de los tumores odontogénicos epiteliales diagnosticados histológicamente en un período de 41 años en una población brasileña según edad, sexo y la zona afectada y comparar estos datos con estudios anteriores de otros países. Los datos de los tumores odontogénicos epiteliales diagnosticados fueron obtenidos de los archivos del Laboratorio de Patología Oral de la Universidad Federal de Rio Grande do Norte, Natal, RN, Brasil, e introducidos en un formulario estandarizado para comparaciones futuras. Las características clínicas obtenidas a partir de los registros de los pacientes y los portaobjetos de microscopio fueron revisados de acuerdo a la clasificación de la Organización Mundial de la Salud 1992. Se informó de un total de 156 tumores epiteliales odontogénicas. De estos, todos eran benignos. Ameloblastoma fue el tipo más frecuente (85,9 %), seguido por el tumor odontogénico adenomatoide (10,9 %) y el tumor odontogénico epitelial calcificante (3,2 %). La edad media de los pacientes fue de 38 años, con un rango amplio (11­80 años). La región posterior de la mandíbula era el sitio anatómico más afectado por esta enfermedad, y no se encontraron diferencias significativas entre sexos en el diagnóstico de los tumores odontogénicos. Se encontró una marcada variación geográfica en las incidencias relativas de diversos tumores odontogénicos epiteliales. Fue particularmente notable en ameloblastomas y tumores odontogénicos adenomatoide, con las incidencias observadas en este estudio siendo a veces similares, y a veces diferentes de los estudios anteriores en otras partes del mundo.

Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Odontogenic Tumors/pathology , Odontogenic Tumors/epidemiology , Skin Neoplasms/pathology , Skin Neoplasms/epidemiology , Brazil/epidemiology , Ameloblastoma/pathology , Ameloblastoma/epidemiology , Mandibular Neoplasms/pathology , Mandibular Neoplasms/epidemiology , Epithelial Cells/pathology , Age and Sex Distribution
Int. braz. j. urol ; 41(5): 849-858, Sept.-Oct. 2015. tab, graf
Article in English | LILACS | ID: lil-767051


ABSTRACT Introduction and Objectives: Reactive Stroma (RStr) is observed in many human cancers and is related to carcinogenesis. The objectives of the present study were to stablish a relationship of the RStr microenvironment with prostate cancer (Pca) through a morphological and molecular characterization, and to identify a possible relationship between RStr with worse prognosis factors and occurrence of malignant prostatic stem cells. Materials and Methods: Forty prostatic samples were selected from men with Pca diagnosis submitted to radical prostatectomy; they were divided in two groups: Group-1 (n=20): samples without reactive stroma; Group-2 (n=20): samples of PCa with intense stroma reaction. Prostatic samples were evaluated for RStr intensity by Masson Trichromic stain and posteriorly submitted to histopathological and immunohistochemistry analysis for antigens: α-actin, vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA, AR, Erα and ERβ. Results: Reactive stroma with intense desmoplastic reactivity was significantly more frequent in intermediate (Gleason 7, 3+4) and high grade tumors (Gleason 7, 4+3). The group with intense stromal reactivity showed significant higher levels of Vimentin, IGF-1, MMP-2, FGF-2, C-Myc, PSCA and ERα. Conclusions: It can be concluded that RStr may be a predictive marker of Pca progression, since it was associated with increase of growth factors, imbalance of androgen and estrogen receptors and presence of malign prostatic stem cells.

Aged , Aged, 80 and over , Humans , Male , Middle Aged , Adenocarcinoma/pathology , Epithelial Cells/pathology , Neoplastic Stem Cells/pathology , Prostatic Neoplasms/pathology , Stromal Cells/pathology , Actins/analysis , Adenocarcinoma/chemistry , Antigens, Neoplasm/analysis , Biomarkers, Tumor/analysis , Disease Progression , DNA-Binding Proteins/analysis , Epithelial Cells/chemistry , Estrogen Receptor alpha/analysis , /analysis , GPI-Linked Proteins/analysis , Immunohistochemistry , Insulin-Like Growth Factor I/analysis , /analysis , Neoplasm Grading , Neoplasm Proteins/analysis , Neoplastic Stem Cells/chemistry , Prostatic Neoplasms/chemistry , Stromal Cells/chemistry , Tumor Microenvironment , Transcription Factors/analysis , Vimentin/analysis
J. appl. oral sci ; 23(5): 472-478, Sept.-Oct. 2015. tab, graf
Article in English | LILACS, BBO | ID: lil-764157


Objective The current study aimed to investigate the β-catenin expression in oral leukoplakia (OL) with different degrees of epithelial dysplasia and normal oral mucosa.Material and Methods Formalin-fixed, paraffin-embedded tissue samples of 39 OL (mild dysplasia n=19, moderate dysplasia n=13, and severe dysplasia n=7), and 10 normal oral mucosa (control group) were submitted to immunohistochemical reactions to anti-β-catenin primary antibody. A qualitative β-catenin analysis was performed based on the percentage of positive cells. The cellular location and the epithelial layer were also considered. The Chi-square test and the Fisher’s exact test were used to verify possible differences in the β-catenin expression among the OL groups. A p-value of <0.05 was considered statistically significant.Results Membranous expression of β-catenin in parabasal and basal layers was gradually lost in the higher degrees of epithelial dysplasia. In normal oral mucosa, β-catenin was detected only in the cytoplasmic membrane. However, a significant increase in cytoplasmic β-catenin could be observed between mild and moderate dysplasia (Fisher Exact test - p<0.001) and between mild and severe dysplasia (p<0.001).Conclusions The β-catenin cytoplasmic expression observed in this study may represent the initial stage of modifications in the E-cadherin-catenin complex, along with morphological cellular changes.

Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Leukoplakia, Oral/metabolism , Mouth Mucosa/metabolism , beta Catenin/analysis , Case-Control Studies , Cell Membrane/pathology , Epithelial Cells/metabolism , Epithelial Cells/pathology , Immunohistochemistry , Leukoplakia, Oral/pathology , Mouth Mucosa/pathology , Paraffin Embedding , Reference Values , Severity of Illness Index
Säo Paulo med. j ; 133(1): 20-27, Jan-Fev/2015. tab, graf
Article in English | LILACS | ID: lil-733012


CONTEXT AND OBJECTIVE: Inadequate Pap smears do not provide satisfactory cell samples for evaluation, thus making it more difficult to detect cervical cytological abnormalities. The objective of this study was to determine the cytological and histological follow-up results from women with inadequate smear reports in primary healthcare centers in Santiago, Chile 2010-2011. DESIGN AND SETTING: Prospective cohort study at primary healthcare clinics in Santiago, Chile. METHODS: The population was taken from the “Cito-Expert" database of 2010. The data were then organized according to the cytological and histological follow-up results of 2,547 women with inadequate cervical cytological reports over the 12-month period. The samples were assigned to groups based on the cause of inadequacy (smears with endocervical cells alone; insufficient, hemorrhagic, inflammatory or poorly fixed samples; insufficient and hemorrhagic samples; or insufficient and inflammatory specimens). The data were analyzed using the “conditional probability tree diagram" and descriptive statistics. RESULT: Half of the women (n = 1,285) met the requirements of the Ministry of Health for repeating these inadequate smears, and 1,104 of these women had normal cytological results (85.9%). The detection rate for cervical lesions according to group ranged ...

CONTEXTO E OBJETIVO: Esfregaços inadequados de Papanicolaou não fornecem amostra satisfatória de células para avaliação, ficando assim mais difícil detectar anomalias citológicas cervicais. O objetivo deste estudo foi determinar os resultados de seguimento citológico e histológico de mulheres com relatórios de esfregaços inadequados nas unidades básicas de saúde em Santiago, Chile, 2010-2011. DESENHO E LOCAL: Estudo prospectivo de coorte em unidades básicas de saúde em Santiago, Chile. MÉTODOS: A população foi selecionada do banco de dados Cito-Expert em 2010. Na sequência, os dados foram organizados pelos resultados de seguimento citológico e histológico de 2.547 mulheres com relatórios inadequados de citologia cervical pelo período de 12 meses. As amostras foram atri-buídas a grupos com base na causa de inadequação (esfregaços com células endocervicais; amostras insuficientes, inflamatórias, ou com má fixação, amostras insuficientes e hemorrágicas, ou espéci-mes insuficientes e inflamatórios). Os dados foram analisados com base no “conditional probability tree diagram" e estatísticas descritivas. RESULTADOS: Metade das mulheres (n = 1.285) preencheram os requisitos ...

Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Middle Aged , Young Adult , Epithelial Cells/cytology , Epithelial Cells/pathology , Outcome and Process Assessment, Health Care/organization & administration , Papanicolaou Test/methods , Vaginal Smears/methods , Chile , Follow-Up Studies , Inflammation/diagnosis , Lost to Follow-Up , Practice Guidelines as Topic , Prospective Studies , Papanicolaou Test/standards , Papanicolaou Test/statistics & numerical data , Specimen Handling , Squamous Intraepithelial Lesions of the Cervix/diagnosis , Vaginal Smears/standards , Vaginal Smears/statistics & numerical data
J. appl. oral sci ; 23(1): 79-86, Jan-Feb/2015. tab, graf
Article in English | LILACS, BBO | ID: lil-741593


Objective The identification of stem cells (SC) remains challenging. In the human oral mucosal epithelium, these cells are believed to be in the basal layer (stem cell niche), but their exact location is unclear. The aim of this study was to examine the dysplastic oral epithelium for these SC-like proteins in order to assess their diagnostic value as biomarkers complementing the histological grading of dysplasia. Material and Methods Thirty oral epithelial dysplasia (OED), 25 oral lichen planus (OLP), 10 oral hyperkeratosis and 5 normal oral epithelium (OE) were immunohistochemically examined for four SC markers [integrin β1, neuron-glial-2 (NG2), notch 1 (N1) and keratin 15 (K15)]. Results Three of four SC markers were heterogeneously detected in all samples. K15 overexpression in the lower two-thirds of severe OED suggests an expanded SC niche. Integrin β1 distribution pattern was not measurably different between OEDs and control. NG2 was almost negative to absent in all samples examined. N1 expression was weak and highly variable in normal and dysplastic epithelium, making it an unreliable epithelial stem cell marker. Conclusions Present findings suggest that these markers were unable to identify individual epithelial stem cells. Instead, subpopulations of cells, most probably stem cells and transit amplifying cells with stem cell-like properties were identified in the dysplastic oral epithelium. The characteristic expressions of K15 might be of diagnostic value for oral dysplasia and should be investigated further. .

Humans , Epithelial Cells/metabolism , Proteins/metabolism , Stem Cells/metabolism , /analysis , Antigens/analysis , Biomarkers/analysis , Epithelial Cells/pathology , Hyperplasia/metabolism , Immunohistochemistry , /analysis , Lichen Planus, Oral/metabolism , Lichen Planus, Oral/pathology , Mouth Mucosa/metabolism , Mouth Mucosa/pathology , Paraffin Embedding , Proteoglycans/analysis , Receptor, Notch1/analysis , Reference Values , Severity of Illness Index , Stem Cells/pathology
SQUMJ-Sultan Qaboos University Medical Journal. 2015; 15 (1): 108-111
in English | IMEMR | ID: emr-160021


This study aimed to analyse trends in epithelial cell abnormalities [ECAs] in cervical cytology at a tertiary care hospital in Kuwait. ECAs in 135,766 reports were compared over three seven-year periods between 1992 and 2012. Conventional Papanicolaou [Pap] smear tests were used in the first two periods and ThinPrep [Hologic Corp., Bedford, Massachusetts, USA] tests were used in the third. Significant increases in satisfactory smears, atypical squamous cells of undetermined significance [ASCUS] and atypical glandular cells of undetermined significance/atypical glandular cells [AGUS/AGCs] were seen in the second and third periods [P <0.001]. No significant increases were observed among low-grade squamous intraepithelial lesions [LSILs] or high-grade squamous intraepithelial lesions [HSILs] [P >0.05]. An increase was noted in carcinomas between the first and second periods although a significant decline was seen in the third [P <0.014]. Satisfactory smears, ASCUS and AGUS/AGC increased during the study period although no significant increases in LSILs, HSILs or carcinomas were observed

Humans , Female , Epithelial Cells/pathology , Tertiary Care Centers , Retrospective Studies , Cytological Techniques
Int. j. morphol ; 32(3): 935-941, Sept. 2014. ilus
Article in English | LILACS | ID: lil-728291


The process of malignancy emergence is associated with the acquisition of the capacity to invade other tissues. Several different biological processes have been described as involved in this process. Specifically, epithelial mesenchymal transition (EMT), a mechanism associated with embryogenesis and wound repair but also with mobility acquisition, is one of the concerned processes. In EMT an epithelial cell loses its epithelial characteristics, its junctions with neighbor cells and with the basal lamina and acquires mobility and mesenchymal characteristics. Also, factors of the tumor microenvironment have been described as involved. Tumor presence triggers a response in the surrounding tissue known as reactive stromal. It shows particular characteristics similar to those found in wound healing stroma: an increase of the fibroblast number and enhancing of the capillary density. The notable difference is the chronicity in the tumoral process. Of a high relevance seems to be the role of activated macrophages with a characteristic phenotype. Finally, cancer associated fibroblasts (CAF) are a type of cells found in tumors, developed from local tissue or possibly from bone marrow. CAF characteristically show a distinct morphology and secrete a high number of metalloproteases allowing tumoral cells advance through the tissue. Additionally, CAF have a direct effect on the survival of the epithelial cells. The three processes are interrelated and metastasis is probably caused by the effect of all of them and probably by other additional factors.

El desarrollo de malignidad está asociado con la adquisición de la capacidad de invadir otros tejidos. Varios procesos diferentes han sido asociados con la aparición de metástasis. Concretamente, la transición epitelio mesénquima (TEM), un mecanismo asociado con embriogénesis y reparación de heridas pero también con adquisición de movilidad, es uno de ellos. En la TEM, una célula epitelial pierde sus características epiteliales, sus uniones con las células vecinas y con la lámina basal y adquiere movilidad y características mesenquemáticas. También han sido asociados factores del microambiente del tumor. La presencia del tumor produce una respuesta en el tejido que lo rodea descrito como estroma reactivo. Sus características son similares a las del estroma de las heridas en proceso de curación: un incremento del número de fibroblastos y un aumento de la densidad de capilares. La gran diferencia es la cronicidad del proceso tumoral. De gran relevancia es el papel de los macrófagos activados que muestran un fenotipo característico. Finalmente, los fibroblastos asociados a cáncer (FAC) son un tipo de células encontradas en tumores, que se desarrollan a partir del tejido local o quizá de la médula ósea. Los FAC, de modo característico muestran una morfología diferente y secretan una gran cantidad de metaloproteasas permitiendo a la célula tumoral avanzar a través del tejido. Además, los FAC ejercen un efecto directo sobre la supervivencia de las células epiteliales. Los tres procesos están interrelacionados y la metástasis es causada probablemente por el efecto de todos ellos y probablemente por otros factores adicionales.

Humans , Epithelial Cells/pathology , Cancer-Associated Fibroblasts/pathology , Neoplasm Metastasis/pathology , Epithelial-Mesenchymal Transition , Neoplasm Invasiveness , Neoplasms/pathology
Braz. j. med. biol. res ; 47(7): 567-575, 07/2014. tab, graf
Article in English | LILACS | ID: lil-712970


Limitations on tissue proliferation capacity determined by telomerase/apoptosis balance have been implicated in pathogenesis of idiopathic pulmonary fibrosis. In addition, collagen V shows promise as an inductor of apoptosis. We evaluated the quantitative relationship between the telomerase/apoptosis index, collagen V synthesis, and epithelial/fibroblast replication in mice exposed to butylated hydroxytoluene (BHT) at high oxygen concentration. Two groups of mice were analyzed: 20 mice received BHT, and 10 control mice received corn oil. Telomerase expression, apoptosis, collagen I, III, and V fibers, and hydroxyproline were evaluated by immunohistochemistry, in situ detection of apoptosis, electron microscopy, immunofluorescence, and histomorphometry. Electron microscopy confirmed the presence of increased alveolar epithelial cells type 1 (AEC1) in apoptosis. Immunostaining showed increased nuclear expression of telomerase in AEC type 2 (AEC2) between normal and chronic scarring areas of usual interstitial pneumonia (UIP). Control lungs and normal areas from UIP lungs showed weak green birefringence of type I and III collagens in the alveolar wall and type V collagen in the basement membrane of alveolar capillaries. The increase in collagen V was greater than collagens I and III in scarring areas of UIP. A significant direct association was found between collagen V and AEC2 apoptosis. We concluded that telomerase, collagen V fiber density, and apoptosis evaluation in experimental UIP offers the potential to control reepithelization of alveolar septa and fibroblast proliferation. Strategies aimed at preventing high rates of collagen V synthesis, or local responses to high rates of cell apoptosis, may have a significant impact in pulmonary fibrosis.

Animals , Male , Apoptosis/physiology , Collagen Type V/biosynthesis , Idiopathic Pulmonary Fibrosis/pathology , Pulmonary Fibrosis/pathology , Telomerase/metabolism , Butylated Hydroxytoluene , Cell Proliferation , Collagen Type I/analysis , Collagen Type II/analysis , Collagen Type V/analysis , Disease Models, Animal , Epithelial Cells/metabolism , Epithelial Cells/pathology , Fluorescent Antibody Technique , Fibroblasts/metabolism , Fibroblasts/pathology , Hydroxyproline/analysis , Immunohistochemistry , In Situ Nick-End Labeling , Mice, Inbred BALB C , Microscopy, Electron , Pulmonary Alveoli/pathology , Pulmonary Alveoli/ultrastructure , Staining and Labeling , Telomerase/isolation & purification
J. bras. patol. med. lab ; 50(3): 234-237, May-Jun/2014. graf
Article in English | LILACS | ID: lil-715621


Tufting enteropathy (TE), also known as intestinal epithelial dysplasia (IED), is a rare congenital enteropathy related to an earlyonset of severe intractable diarrhea due to specific abnormalities of the intestinal epithelium and mutations of the EpCAM gene. TE is characterized by clinical and histological heterogeneity, such as with low or without mononuclear cell infiltration of the lamina propria, and abnormalities of basement membrane. TE can be associated with malformations, other epithelial diseases, or to abnormal enterocytes development and/or differentiation. The authors report a case of a Brazilian child with TE associated with c.556-14A>G mutation in the EpCAM gene (NM_002354.2)...

Enteropatia com formação de tufos epiteliais (ETE), também conhecida como displasia epitelial intestinal (DEI), é uma rara enteropatia congênita relacionada com um início precoce de diarreia intratável grave devido a anormalidades específicas do epitélio intestinal e mutações do gene EpCAM. ETE caracteriza-se por uma heterogeneidade clínica e histológica, como ausência ou leve infiltrado de células mononucleares na lâmina própria e anormalidades de membrana basal. Pode ser associada a malformações, outras doenças epiteliais ou anormalidades no desenvolvimento/na diferenciação dos enterócitos. Os autores relatam um caso de ETE, em uma criança brasileira, associada à mutação c.556-14A> g do gene EPCAM (NM_002354.2)...

Humans , Female , Child , Epithelial Cells/pathology , Intestinal Diseases/genetics , Cell Adhesion Molecules/genetics , Diarrhea, Infantile , Intestinal Mucosa/pathology