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1.
Article in English | WPRIM | ID: wpr-1011009

ABSTRACT

Liver fibrosis is a dynamic wound-healing response characterized by the agglutination of the extracellular matrix (ECM). Si-Wu-Tang (SWT), a traditional Chinese medicine (TCM) formula, is known for treating gynecological diseases and liver fibrosis. Our previous studies demonstrated that long non-coding RNA H19 (H19) was markedly upregulated in fibrotic livers while its deficiency markedly reversed fibrogenesis. However, the mechanisms by which SWT influences H19 remain unclear. Thus, we established a bile duct ligation (BDL)-induced liver fibrosis model to evaluate the hepatoprotective effects of SWT on various cells in the liver. Our results showed that SWT markedly improved ECM deposition and bile duct reactions in the liver. Notably, SWT relieved liver fibrosis by regulating the transcription of genes involved in the cytoskeleton remodeling, primarily in hepatic stellate cells (HSCs), and influencing cytoskeleton-related angiogenesis and hepatocellular injury. This modulation collectively led to reduced ECM deposition. Through extensive bioinformatics analyses, we determined that H19 acted as a miRNA sponge and mainly inhibited miR-200, miR-211, and let7b, thereby regulating the above cellular regulatory pathways. Meanwhile, SWT reversed H19-related miRNAs and signaling pathways, diminishing ECM deposition and liver fibrosis. However, these protective effects of SWT were diminished with the overexpression of H19 in vivo. In conclusion, our study elucidates the underlying mechanisms of SWT from the perspective of H19-related signal networks and proposes a potential SWT-based therapeutic strategy for the treatment of liver fibrosis.


Subject(s)
Humans , RNA, Long Noncoding/genetics , Liver Cirrhosis/genetics , Liver/metabolism , Hepatic Stellate Cells/pathology , MicroRNAs/metabolism , Extracellular Matrix/metabolism , Drugs, Chinese Herbal
2.
Chinese Journal of Biotechnology ; (12): 942-960, 2023.
Article in Chinese | WPRIM | ID: wpr-970415

ABSTRACT

Collagen, which widely exists in skin, bone, muscle and other tissues, is a major structural protein in mammalian extracellular matrix. It participates in cell proliferation, differentiation, migration and signal transmission, plays an important role in tissue support and repair and exerts a protective effect. Collagen is widely used in tissue engineering, clinical medicine, food industry, packaging materials, cosmetics and medical beauty due to its good biological characteristics. This paper reviews the biological characteristics of collagen and its application in bioengineering research and development in recent years. Finally, we prospect the future application of collagen as a biomimetic material.


Subject(s)
Animals , Collagen/analysis , Tissue Engineering/methods , Extracellular Matrix/metabolism , Biomimetic Materials/chemistry , Bone and Bones , Tissue Scaffolds , Mammals/metabolism
3.
Article in Chinese | WPRIM | ID: wpr-985450

ABSTRACT

Scarring, naturally induced by fibroblasts(Fb) during wound healing, is an essential process in response to repair damaged tissue. Excessive Fb proliferation which produces the excessive collagen deposition, including increased extracellular matrix synthesis or insufficient decomposition, typically contributes to hypertrophic scar(HS) formation. Although exact mechanisms of HS are not yet fully understood, it is generally believed that dysfunction of Fb and regulation of signal pathways play an important role in HS formation. Biologically, Fb function is affected by various factors such as cytokines, extracellular matrix and itself. In addition, modifications of miRNA, ceRNA, lncRNA, peptides and histones participate in HS formation by affecting the biological function of Fb. Despite the clinical importance, very few therapeutic modalities are available to prevent HS. To achieve this, a deeper characterization of Fb is required to identify mechanisms of HS. To the aspect of HS prevention and treatment, we review recent findings, concentrating on Fb function and collagen secretion. The objective of this article is to frame the current understanding, gain the deeper insights into Fb function, and provide the more comprehensive cognition and perspective for prevention and treatment of HS.


Subject(s)
Humans , Cicatrix, Hypertrophic/metabolism , Collagen/therapeutic use , Fibroblasts , Signal Transduction , Extracellular Matrix/metabolism
4.
Article in English | WPRIM | ID: wpr-982697

ABSTRACT

Diabetic kidney disease (DKD) is the most common complication of diabetes mellitus (DM). Qianjin Wenwu decoction (QWD), a well-known traditional Korean medicine, has been used for the treatment of DKD, with satisfactory therapeutic effects. This study was designed to investigate the active components and mechanisms of action of QWD in the treatment of DKD. The results demonstrated that a total of 13 active components in five types were found in QWD, including flavonoids, flavonoid glycosides, phenylpropionic acids, saponins, coumarins, and lignins. Two key proteins, TGF-β1 and TIMP-1, were identified as the target proteins through molecular docking. Furthermore, QWD significantly suppressed Scr and BUN levels which increased after unilateral ureteral obstruction (UUO). Hematoxylin & eosin (H&E) and Masson staining results demonstrated that QWD significantly alleviated renal interstitial fibrosis in UUO mice. We also found that QWD promoted ECM degradation by regulating MMP-9/TIMP-1 homeostasis to improve renal tubulointerstitial fibrosis and interfere with the expression and activity of TGF- β1 in DKD treatment. These findings explain the underlying mechanism of QWD for the treatment of DKD, and also provide methodological reference for investigating the mechanism of traditional medicine in the treatment of DKD.


Subject(s)
Rats , Mice , Animals , Ureteral Obstruction/metabolism , Kidney/metabolism , Tissue Inhibitor of Metalloproteinase-1/metabolism , Molecular Docking Simulation , Rats, Sprague-Dawley , Kidney Diseases/drug therapy , Extracellular Matrix/metabolism , Flavonoids/metabolism , Fibrosis
6.
Frontiers of Medicine ; (4): 56-82, 2022.
Article in English | WPRIM | ID: wpr-929195

ABSTRACT

Contributing to organ formation and tissue regeneration, extracellular matrix (ECM) constituents provide tissue with three-dimensional (3D) structural integrity and cellular-function regulation. Containing the crucial traits of the cellular microenvironment, ECM substitutes mediate cell-matrix interactions to prompt stem-cell proliferation and differentiation for 3D organoid construction in vitro or tissue regeneration in vivo. However, these ECMs are often applied generically and have yet to be extensively developed for specific cell types in 3D cultures. Cultured cells also produce rich ECM, particularly stromal cells. Cellular ECM improves 3D culture development in vitro and tissue remodeling during wound healing after implantation into the host as well. Gaining better insight into ECM derived from either tissue or cells that regulate 3D tissue reconstruction or organ regeneration helps us to select, produce, and implant the most suitable ECM and thus promote 3D organoid culture and tissue remodeling for in vivo regeneration. Overall, the decellularization methodologies and tissue/cell-derived ECM as scaffolds or cellular-growth supplements used in cell propagation and differentiation for 3D tissue culture in vitro are discussed. Moreover, current preclinical applications by which ECM components modulate the wound-healing process are reviewed.


Subject(s)
Humans , Cell Differentiation , Cell Proliferation , Decellularized Extracellular Matrix , Extracellular Matrix/metabolism , Mesenchymal Stem Cells , Tissue Engineering/methods , Tissue Scaffolds/chemistry
7.
Article in Chinese | WPRIM | ID: wpr-927874

ABSTRACT

Objective To explore the effect of overwork (OW) on extracellular matrix of arterial vessel wall in rats. Methods Random number grouping method was employed to assign 18 Sprague-Dawley rats into three groups(n=6):the control group(no special treatment),group OW(forced swimming twice a day for 15 days),and sleep deficiency(SD)+OW group(in addition to forced swimming twice a day,the rats were put on the platforms in water to limit sleep for 15 days).On the 16th day,the abdominal aorta and common carotid artery were collected after blood sampling from heart under deep anesthesia.A part of the abdominal aorta sample was taken for Masson staining of collagen fiber,and Verhoeff-Van Gieson staining was carried out for the elastic fiber of common carotid artery.Image J was employed for the quantitative analysis of collagen fiber and elastic fiber content.The expression of collagen 1(Col-1) protein was quantified by immunohistochemistry and the ultrastructure of vascular matrix was examined by transmission electron microscopy.The other part of the abdominal aorta sample was used to determine the mRNA levels of matrix metalloproteinase(MMP)-1,MMP-2,MMP-9,tissue inhibitor of metalloproteinases-1(TIMP-1),and Col-1 by quantitative real-time polymerase chain reaction. Results Compared with that in control group,the content of collagen fiber in groups OW and SD+OW had no significant change(all P>0.05);the content of elastic fiber in groups OW and SD+OW decreased(all P<0.001) and had no significant difference between each other(P>0.05).The vascular vessel wall of group OW showed slight fiber breakage,while that of group SD+OW presented wormhole-like or spongy fiber fragmentation.The mRNA levels of MMP-1 and MMP-2 in groups OW and SD+OW had no significant difference between each other(P>0.05) but were higher than that in control group(all P<0.001).The mRNA levels of MMP-9 and TIMP-1 had no significant difference among the three groups(all P>0.05).Groups OW and SD+OW had lower mRNA level(all P<0.001) and protein level(all P<0.001) of Col-1 than control group,while the mRNA and protein levels of Col-1 had no significant difference between groups OW and SD+OW(P>0.05). Conclusion OW can reduce the content of Col-1 and elastic fibers in the extracellular matrix of arterial vessels,destroy the elastic lamina of vascular wall,up-regulate the expression of MMP-1 and MMP-2,thereby injuring arterial vessels.


Subject(s)
Animals , Rats , Collagen Type I , Extracellular Matrix/metabolism , Matrix Metalloproteinase 1/metabolism , Matrix Metalloproteinase 2/metabolism , Matrix Metalloproteinase 9/metabolism , RNA, Messenger/genetics , Rats, Sprague-Dawley , Tissue Inhibitor of Metalloproteinase-1/metabolism
8.
Chinese Journal of Burns ; (6): 691-696, 2022.
Article in Chinese | WPRIM | ID: wpr-940976

ABSTRACT

Wound repair is a highly coordinated and mutually regulated complex process involving various kinds of cells, extracellular matrices and cytokines. A variety of growth factors play an important regulatory role in wound healing, and it is critical to achieve effective delivery and sustained function of growth factors. In recent years, the application of biomaterials in tissue engineering has shown great potential, and the effective delivery of growth factors by biomaterials has attracted increasing attention. Based on this, this paper introduces the mechanism of related growth factors in the process of wound healing, focusing on the recent progress of biomaterial delivery of growth factors to accelerate wound healing, in order to provide new enlightenment for clinical wound treatment.


Subject(s)
Biocompatible Materials/metabolism , Extracellular Matrix/metabolism , Intercellular Signaling Peptides and Proteins/therapeutic use , Tissue Engineering , Wound Healing
9.
J. coloproctol. (Rio J., Impr.) ; 41(1): 63-69, Jan.-Mar. 2021. tab, graf
Article in English | LILACS | ID: biblio-1286971

ABSTRACT

Abstract Objective Type-I collagen (Col-I) is one of the main macromolecules of the extracellular matrix, and it is involved in the desmoplastic stromal reaction, an indicator of worse prognosis in cases of colorectal cancer (CRC). The purpose of the present study was to investigate Col-I expression in cases of CRC and adenoma and to correlate with the clinical data and the data regarding the lifestyle of the patients. Methods A retrospective study including 22 patients with adenoma and 15 with CRC treated at a coloproctology service. The clinical and lifestyle data were obtained through medical records, and Col-I expression was investigated by immunohistochemistry. Results Women represented most cases of adenoma (63.64%), whereas CRC was found mainly in men (73.33%) (p=0.0448). Immunoexpression of Col-I showed a basement membrane thickening in areas of lining of epithelium and around the glands in both lesions. The cases of CRC had a quite evident fibrosis process in the stroma. The quantitative analysis demonstrated a higher protein expression in CRCs compared to adenomas (p=0.0109), as well as in female patients (p=0.0214), patients aged ≥ 50 years (p=0.0400), and in those with a positive family history of colorectal disease (p=0.0292). These results suggested a remodeling of the microenvironment of the Worked developed at the Department of Morphology, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, ES, Brazil. Conclusion The immunohistochemical analysis encourages the performance of more comprehensive studies to ascertain if our results could be a tool for the diagnosis and monitoring of the patients.


Resumo Objetivo O colágeno tipo I (Col-I) é uma das principais macromoléculas da matriz extracelular, e está envolvido na reação desmoplástica estromal, um indicador de pior prognóstico em casos de câncer colorretal (CCR). O objetivo foi investigar a expressão do Col-I emcasos de CCR e adenoma, e correlacioná-la comdados clínicos e de estilo de vida dos pacientes. Metodologia Foi realizado umestudoretrospectivo com22pacientes comadenoma e 15 comCCR tratadosemumserviço de coloproctologia.Os dados dos pacientes foramobtidos dos prontuários médicos, e a expressão do Col-I foi investigada por imunohistoquímica. Resultados As mulheres representaram a maioria dos casos de adenomas (63,64%), enquanto o CCR (73,33%) (p=0,0448) foi mais comum entre os homens. A imunoexpressão de Col-I mostrou espessamento da membrana basal em áreas de revestimento do epitélio e em volta de glândulas em ambas as lesões. O CCR apresentou fibrose no estroma. As análises quantitativas demonstraram maior expressão proteica no CCR (p=0,0109), assim como em mulheres (p=0,0214), pacientes com idade ≥ 50 anos (p=0,0400), e em pacientes com histórico positivo de doença colorretal na família (p=0,0292). Estes resultados sugerem a remodelação do microambiente tumoral na carcinogênese do CCR. As correlações clínico-patológicas positivas mostram uma ligação plausível entre o perfil do paciente e os achados imunohistoquímcos, o que indica uma possível forma de estratificação dos pacientes. Conclusão As análises imunohistoquímicas estimulam a execução de estudos mais abrangentes para confirmar se nossos resultados poderão ser uma ferramenta para o diagnóstico e o monitoramento dos pacientes.


Subject(s)
Humans , Male , Female , Colorectal Neoplasms/metabolism , Colorectal Neoplasms/pathology , Adenocarcinoma/diagnosis , Biomarkers, Tumor/metabolism , Gene Expression Regulation, Neoplastic , Collagen Type I/genetics , Extracellular Matrix/metabolism , Tumor Microenvironment/immunology
10.
Rev. bras. oftalmol ; 79(1): 71-80, Jan.-Feb. 2020. tab, graf
Article in Portuguese | LILACS | ID: biblio-1092653

ABSTRACT

Resumo Atualmente a membra amniótica (MA) tem obtido importância devido à comprovada capacidade de reduzir inflamação, auxiliar a cicatrização e epitelização, possuindo propriedades antimicrobianas e antivirais, além de baixa imunogenicidade. As indicações de seu uso na oftalmologia têm aumentado muito nas duas últimas décadas. Objetivo: Descrever a estrutura básica e as propriedades biológicas da MA em relação aos componentes da sua matriz extracelular e fatores de crescimento, as consequências de diferentes técnicas empregadas na sua preservação e esterilização, métodos para remoção do epitélio e a comparação dos custos dos diferentes meios de conservação atualmente empregados. Métodos: Pesquisa nas bases de dados do Portal da Biblioteca Virtual em Saúde (BVS), Pubmed, Cochrane, Scielo e Lilacs com as palavras-chave: membrana amniótica, transplante, reconstrução da córnea, doenças da conjuntiva. Resultados: A literatura é vasta na descrição dos efeitos de diversos agentes e técnicas na preparação da MA, dentre elas sua preservação, esterilização e desepitelização. A membrana desnuda tem sido a escolha para a reconstrução da superfície ocular, pois facilita a cicatrização. Em relação aos agentes conservantes, o glicerol é o meio mais utilizado mundialmente pelo baixo custo e facilidade de manuseio. Conclusão: A comparação das diversas técnicas nos guia na elaboração de protocolos de preparo da MA para uso oftalmológico. A membrana desnuda facilita a cicatrização em relação a com células epiteliais. O glicerol é o meio de conservação mais utilizado pelo baixo custo e facilidade de manuseio.


Abstract Currently, the amniotic membrane (AM) has obtained importance due to its ability to reduce inflammation, helping in the healing and epithelialization processes, having antimicrobial and antiviral properties and low immunogenicity. Its indications in ophthalmology have increased considerably in the past two decades. Objective: To describe the basic structure and biological properties of the AM, the components of the extracellular matrix and growth factors, the consequences of different techniques used in its preservation, and sterilization methods for the epithelium removal. To compare the costs of the different preservation solutions currently employed. Study design: literature review. Methods: Research in BVS databases, PubMed, Cochrane, Scielo and Lilacs with keywords: amniotic membrane transplantation, corneal reconstruction, conjunctival diseases. Results: The literature is vast in describing the effects of different agents and techniques used in the preparation of MA, including its preservation, sterilization and desepithelization. The naked membrane is the choice to reconstruct the ocular surface, as it facilitates the healing course. Regarding the preservatives, glycerol is the most used worldwide due its low cost and easy handling. Conclusion: Comparing different techniques guides us in developing a MA preparation protocol for ophthalmic use. The naked membrane facilitates the healing process compared with the presence of epithelial cells. The glycerol is the most used preservation method because of its low cost and easy handling.


Subject(s)
Humans , Tissue Preservation/methods , Conjunctival Diseases/surgery , Corneal Diseases/surgery , Tissue and Organ Harvesting/methods , Eye Diseases/surgery , Amnion/transplantation , Tissue Banks/standards , Tissue Donors/supply & distribution , Wound Healing , Biological Dressings/standards , Biological Products/standards , Tissue and Organ Procurement/standards , Cryopreservation/methods , Sterilization/methods , Collagen/metabolism , Intercellular Signaling Peptides and Proteins/metabolism , Extracellular Matrix/metabolism , Amnion/cytology , Amnion/microbiology , Amnion/ultrastructure
11.
Article in English | WPRIM | ID: wpr-881038

ABSTRACT

Due to the poor repair ability of cartilage tissue, regenerative medicine still faces great challenges in the repair of large articular cartilage defects. Quercetin is widely applied as a traditional Chinese medicine in tissue regeneration including liver, bone and skin tissues. However, the evidence for its effects and internal mechanisms for cartilage regeneration are limited. In the present study, the effects of quercetin on chondrocyte function were systematically evaluated by CCK8 assay, PCR assay, cartilaginous matrix staining assays, immunofluorescence assay, and western blotting. The results showed that quercetin significantly up-regulated the expression of chondrogenesis genes and stimulated the secretion of GAG (glycosaminoglycan) through activating the ERK, P38 and AKT signalling pathways in a dose-dependent manner. Furthermore, in vivo experiments revealed that quercetin-loaded silk protein scaffolds dramatically stimulated the formation of new cartilage-like tissue with higher histological scores in rat femoral cartilage defects. These data suggest that quercetin can effectively stimulate chondrogenesis in vitro and in vivo, demonstrating the potential application of quercetin in the regeneration of cartilage defects.


Subject(s)
Animals , Rats , Cartilage/cytology , Chondrocytes/drug effects , Chondrogenesis/drug effects , Extracellular Matrix/metabolism , Quercetin/pharmacology , Signal Transduction/drug effects , Tissue Scaffolds
12.
Mem. Inst. Oswaldo Cruz ; 115: e200007, 2020. graf
Article in English | LILACS, SES-SP | ID: biblio-1135242

ABSTRACT

BACKGROUND Behavioral and neurochemical alterations associated with toxoplasmosis may be influenced by the persistence of tissue cysts and activation of an immune response in the brain of Toxoplasma gondii-infected hosts. The cerebral extracellular matrix is organised as perineuronal nets (PNNs) that are both released and ensheath by some neurons and glial cells. There is evidences to suggest that PNNs impairment is a pathophysiological mechanism associated with neuropsychiatric conditions. However, there is a lack of information regarding the impact of parasitic infections on the PNNs integrity and how this could affect the host's behavior. OBJECTIVES In this context, we aimed to analyse the impact of T. gondii infection on cyst burden, PNNs integrity, and possible effects in the locomotor activity of chronically infected mice. METHODS We infected mice with T. gondii ME-49 strain. After thirty days, we assessed locomotor performance of animals using the open field test, followed by evaluation of cysts burden and PNNs integrity in four brain regions (primary and secondary motor cortices, prefrontal and somesthetic cortex) to assess the PNNs integrity using Wisteria floribunda agglutinin (WFA) labeling by immunohistochemical analyses. FINDINGS AND MAIN CONCLUSIONS Our findings revealed a random distribution of cysts in the brain, the disruption of PNNs surrounding neurons in four areas of the cerebral cortex and hyperlocomotor behavior in T. gondii-infected mice. These results can contribute to elucidate the link toxoplasmosis with the establishment of neuroinflammatory response in neuropsychiatric disorders and to raise a discussion about the mechanisms related to changes in brain connectivity, with possible behavioral repercussions during chronic T. gondii infection.


Subject(s)
Animals , Mice , Cerebellum/metabolism , Toxoplasmosis/pathology , Toxoplasmosis, Animal , Extracellular Matrix/metabolism , Motor Neurons/cytology , Neurons/pathology , Toxoplasma , Cerebellum/cytology , Toxoplasmosis/metabolism , Disease Models, Animal , Motor Neurons/metabolism , Neurons/metabolism
13.
Int. j. morphol ; 37(1): 111-117, 2019. tab, graf
Article in English | LILACS | ID: biblio-990014

ABSTRACT

SUMMARY: Arterial obstruction in small diameter (<6 mm) vessels are many times treated with grafts, however autologous aren't always available and synthetic have a high rate of complications. Decellularization of umbilical arteries may provide a solution, but the ideal method is debatable. We compare effectiveness between SDS and Triton X-100. Umbilical cords obtained from full term pregnancies with normal development and no evident complications in the newborn, were micro-dissected within 12 h and stored in phosphate buffered saline without freezing. Arteries were then processed for decellularization using 0.1 % and 1 % SDS, and 1 % Triton X100 protocols. Evaluation of cellular and nuclear material, collagen fibers, elastic fibers, and glycosoaminoglycans of the extracellular matrix (ECM) were evaluated as well as morphometric analysis under histological and immunohistochemical techniques. Triton X-100 was ineffective, preserving nuclear remains identified by immunofluorescence, had the most notable damage to elastic fibers, and decrease in collagen. SDS effectively eliminated the nuclei and had a less decrease in elastic fibers and collagen. Laminin was preserved in all groups. No significant differences were identified in luminal diameters; however the middle layer decreased due to decellularization of muscle cells. In conclusion, 0.1 % SDS decellularization was the most effective in eliminating cells and preserving the main components of the ECM.


RESUMEN: La obstrucción arterial en vasos de pequeño diámetro (<6 mm) se trata muchas veces con injertos, sin embargo, los autólogos no siempre están disponibles y los sintéticos tienen una alta tasa de complicaciones. La descelularización de las arterias umbilicales puede proporcionar una solución, pero el método ideal es discutible. Comparamos la efectividad entre los métodos SDS y Triton X-100. Cordones umbilicales obtenidos a partir de embarazos a término con evolución normal y sin complicaciones evidentes del recién nacido, se microdiseccionaron en 12 horas y se almacenaron en solución salina con fosfato sin congelación. Las arterias se procesaron luego para la descelularización usando los protocolos de SDS al 0,1 % y 1 %, y Triton X-100 al 1 %. Se realizó la evaluación de material celular y nuclear, fibras de colágeno, fibras elásticas y glucosoaminoglicanos de la matriz extracelular (MEC), así como el análisis morfométrico bajo técnicas histológicas e inmunohistoquímicas. Triton X-100 fue ineficaz, conservando los restos nucleares identificados por inmunofluorescencia, tuvo el daño más notable a las fibras elásticas y la disminución del colágeno. SDS efectivamente eliminó los núcleos y tuvo una disminución menor en las fibras elásticas y el colágeno. Laminina fue preservado en todos los grupos. No se identificaron diferencias significativas en los diámetros luminales; sin embargo, la capa media disminuyó debido a la descelularización de las células musculares. la descelularización con SDS al 0,1 % fue la más efectiva para eliminar células y preservar los principales componentes de la MEC.


Subject(s)
Humans , Umbilical Arteries/cytology , Umbilical Arteries/metabolism , Tissue Engineering/methods , Extracellular Matrix/metabolism , Umbilical Arteries/transplantation , Umbilical Cord , Immunohistochemistry , Cell Separation , Fluorescent Antibody Technique , Collagen , Vascular Grafting
14.
Biol. Res ; 52: 23, 2019. graf
Article in English | LILACS | ID: biblio-1011425

ABSTRACT

BACKGROUND: Conjunctival filtering bleb scar formation is the main reason for the failure of glaucoma filtration surgery. Cytoglobin (Cygb) has been reported to play an important role in extracellular matrix (ECM) remodeling, fibrosis and tissue damage repairing. This study aimed to investigate the role of Cygb in anti-scarring during excessive conjunctival wound healing after glaucoma filtration surgery. METHODS: Cygb was overexpressed in human tenon fibroblasts (hTFs) by transfecting hTFs with lentiviral particles encoding pLenti6.2-FLAG-Cygb. Changes in the mRNA and protein levels of fibronectin, collagen I, collagen III, TGF-ß1, and HIF1α were determined by RT-PCR and western blotting respectively. RESULTS: After Cygb overexpression, hTFs displayed no significant changes in visual appearance and cell counts compared to controls. Whereas, Cygb overexpression significantly decreased the mRNA and protein expression levels of collagen I, collagen III and fibronectin compared with control (p < 0.01). There was also a statistically significant decrease in the mRNA and protein levels of TGF-ß1 and HIF-1α in hTFs with overexpressed Cygb compared with control group (p < 0.05). CONCLUSION: Our study provided evidence that overexpression of Cygb decreased the expression levels of fibronectin, collagen I, collagen III, TGF-ß1 and HIF-1α in hTFs. Therefore, therapies targeting Cygb expression in hTFs may pave a new way for clinicians to solve the problem of post-glaucoma surgery scarring.


Subject(s)
Humans , Extracellular Matrix/metabolism , Tenon Capsule/metabolism , Fibroblasts/metabolism , Cytoglobin/metabolism , RNA, Messenger/analysis , Collagen/analysis , Fibronectins/analysis , Vascular Endothelial Growth Factor A/metabolism , Extracellular Matrix/drug effects , Cytoglobin/pharmacology
15.
Braz. j. microbiol ; 49(3): 675-682, July-Sept. 2018. graf
Article in English | LILACS | ID: biblio-951801

ABSTRACT

Abstract Aspergillus fumigatus is an opportunistic saprobe fungus that accounts for 90% of cases of pulmonary aspergillosis in immunosuppressed patients and is known for its angiotropism. When it reaches the respiratory tract, A. fumigatus interacts with structural components and blood vessels of the lungs, such as elastin. To understand the effect of this structural component, we examined the effect of elastin on the production and development of the biofilm of A. fumigatus. In RPMI containing 10 mg/mL of elastin, a significant increase (absorbance p < 0.0001; dry weight p < 0.0001) in the production of biofilm was observed in comparison to when RPMI was used alone, reaching a maximum growth of 18.8 mg (dry weight) of biofilm in 72 h. In addition, elastin stimulates the production (p = 0.0042) of extracellular matrix (ECM) and decreases (p = 0.005) the hydrophobicity during the development of the biofilm. These results suggest that elastin plays an important role in the growth of A. fumigatus and that it participates in the formation of thick biofilm.


Subject(s)
Humans , Aspergillosis/metabolism , Aspergillosis/microbiology , Aspergillus fumigatus/physiology , Elastin/metabolism , Biofilms , Extracellular Matrix/metabolism , Aspergillus fumigatus/genetics , Host-Pathogen Interactions
16.
Rev. Asoc. Méd. Argent ; 131(2): 7-26, jun. 2018. tab, ilus
Article in Spanish | LILACS | ID: biblio-973098

ABSTRACT

Se revisan los nuevos conocimientos sobre la matriz extracelular (MEC), que han permitido descubrir su importante rol en la cicatrización de las heridas cutáneas. Se describen sus características morfofisiológicas y cómo interviene en la curación de las heridas cutáneas. Se presentan cuatro casos clínicos en los que se aplicó este enfoque terapéutico: los sustitutos de piel y la “cura húmeda”.


We review the new knowledge about the extracellular matrix (ECM) that has allowed us to discover its important role in the healing of cutaneous wounds. The morphophysiological characteristics of ECM and its role in the healing of cutaneous wounds are described. Four clinical cases are presented where this therapeutic approach was applied: the skin substitutes and the “moist wound healing”.


Subject(s)
Male , Female , Humans , Adolescent , Middle Aged , Aged , Extracellular Matrix/metabolism , Extracellular Matrix/genetics , Skin/injuries , Wound Healing/physiology , Cell Differentiation , Electromagnetic Radiation , Regenerative Medicine
17.
Article in English | WPRIM | ID: wpr-1010432

ABSTRACT

OBJECTIVE@#To construct a novel non-viral vector loaded with growth and differentiation factor-5 (GDF-5) plasmid using chitosan, hyaluronic acid, and chondroitin sulfate for osteoarthritis (OA) gene therapy.@*METHODS@#Nano-microspheres (NMPs) were prepared by mixing chitosan, hyaluronic acid, and chondroitin sulfate. GDF-5 plasmid was encapsulated in the NMPs through electrostatic adsorption. The basic characteristics of the NMPs were observed, and then they were co-cultured with chondrocytes to observe their effects on extracellular matrix (ECM) protein expression. Finally, NMPs loaded with GDF-5 were injected into the articular cavities of rabbits to observe their therapeutic effects on OA in vivo.@*RESULTS@#NMPs exhibited good physicochemical properties and low cytotoxicity. Their average diameter was (0.61±0.20) μm, and encapsulation efficiency was (38.19±0.36)%. According to Cell Counting Kit-8 (CCK-8) assay, relative cell viability was 75%-99% when the total weight of NMPs was less than 560 μg. Transfection efficiency was (62.0±2.1)% in a liposome group, and (60.0±1.8)% in the NMP group. There was no significant difference between the two groups (P>0.05). Immunohistochemical staining results suggested that NMPs can successfully transfect chondrocytes and stimulate ECM protein expression in vitro. Compared with the control groups, the NMP group significantly promoted the expression of chondrocyte ECM in vivo (P<0.05), as shown by analysis of the biochemical composition of chondrocyte ECM. When NMPs were injected into OA model rabbits, the expression of ECM proteins in chondrocytes was significantly promoted and the progression of OA was slowed down.@*CONCLUSIONS@#Based on these data, we think that these NMPs with excellent physicochemical and biological properties could be promising non-viral vectors for OA gene therapy.


Subject(s)
Animals , Rabbits , Cell Differentiation , Cell Survival/drug effects , Chitosan/chemistry , Chondrocytes/cytology , Chondroitin Sulfates/chemistry , Drug Carriers , Extracellular Matrix/metabolism , Genetic Therapy/methods , Growth Differentiation Factor 5/genetics , Hyaluronic Acid/chemistry , Microspheres , Nanomedicine , Osteoarthritis/therapy , Plasmids/metabolism
18.
Clinics ; 73(supl.1): e551s, 2018. graf
Article in English | LILACS | ID: biblio-952836

ABSTRACT

Infection with human papillomaviruses is associated with a series of benign and malignant hyperproliferative diseases that impose a heavy burden on human populations. A subgroup of mucosal human papillomavirus types are associated with the majority of cervical cancers and a relevant fraction of vulvar, vaginal, anal, penile and head and neck carcinomas. Human papillomaviruses mediate cell transformation by the expression of two pleiotropic oncoproteins that alter major cellular regulatory pathways. However, these viruses are not complete carcinogens, and further alterations within the infected cells and in their microenvironment are necessary for tumor establishment and progression. Alterations in components of the extracellular matrix for instance, matrix metalloproteinases and some of their regulators such as tissue inhibitors of metalloproteinases, have been consistently reported in human papillomaviruses-associated diseases. Matrix metalloproteinases function by remodeling the extracellular matrix and alterations in their expression levels and/or activity are associated with pathological processes and clinical variables including local tumor invasion, metastasis, tumor relapse and overall patient prognosis and survival. In this review we present a summarized discussion on the current data concerning the impact of human papillomavirus infection on the activity and expression of extracellular matrix components. We further comment on the possibility of targeting extracellular matrix molecules in experimental treatment protocols.


Subject(s)
Humans , Male , Female , Cell Transformation, Neoplastic/metabolism , Papillomavirus Infections/metabolism , Extracellular Matrix/metabolism , Papillomavirus Infections/virology , Genital Neoplasms, Female/virology , Genital Neoplasms, Male/virology , Head and Neck Neoplasms/virology
19.
Rev. bras. epidemiol ; 18(supl.2): 45-56, Out.-Dez. 2015. tab
Article in English | LILACS | ID: lil-776703

ABSTRACT

RESUMO: Objetivo: Comparar a prevalência de fumantes atuais de tabaco na população brasileira e nas unidades federativas, em adultos (≥ 18 anos), considerando dois inquéritos populacionais realizados em 2008 e 2013. Métodos: São comparadas as prevalências de fumantes atuais de tabaco no Brasil e nas unidades federativas analisando dados da Pesquisa Nacional de Amostra de Domicílios, de 2008, e da Pesquisa Nacional de Saúde, de 2013. Foram calculados a variação percentual no período e o valor de p. Resultados: A prevalência de fumantes atual de tabaco reduziu -19% no período, saindo de 18,2% (2008) para 14,7% (2013). O declínio ocorreu em todas as regiões, área urbana e rural e na maioria dos estados. A redução foi de -17,5% para os homens e -20,7% para as mulheres, reduziu em todas as faixas de idade, sendo a maior redução entre 25 e 39 anos; também reduziu para todas as categorias de raça/cor, sendo as prevalências mais altas entre pretos e pardos. Declinou também em todas as faixas de escolaridade, sendo maior a redução nas faixas de menor escolaridade. Em 2013, as prevalências para população com menor escolaridade foram de 19,7% e de 8,7% para quem tem nível superior completo. Conclusão: Ocorreu uma redução média de cerca de 19% no consumo do tabaco no Brasil e nos estados brasileiros, em ambos o sexos, todas faixas de idade e raça/cor. O consumo do tabaco no país é um dos mais baixos do mundo e declinou de forma significativa, o que pode ser atribuído a políticas de controle, regulação e prevenção.


ABSTRACT: Objective: To compare current tobacco smoking prevalence in the Brazilian population and the federal states in adults (aged ≥ 18 years), using the National Household Survey 2008 and National Health Survey, 2013. Methods: Using data from two national surveys conducted in 2008 and 2013, the paper examines the current tobacco smoking prevalence in Brazil at the national level and at the federal state level. We calculated the percentage change for the period. Results: Overall, results show -19% reduction in current tobacco smoking prevalence from 18.5% (2008) to 14.7% (2013). Results also show a significant percentage decline in smoking prevalence across geographic regions and demographic characteristics including gender, race, age and education levels. The decline occurred in all regions, urban and rural areas, and in most states. The reduction was -17.5% for men and -20.7% for women, having occurred in all age groups, with the greatest reduction in the group from 25 to 39 years of age; in all categories of race/color, a higher prevalence was found among the blacks and browns. It also declined in all the levels of schooling, with a higher reduction in lower education levels. In 2013, the prevalence for people with less education was 19.7% and 8.7% for those with college degrees. Conclusion: There was an average reduction of about 19% in tobacco consumption in Brazil and the Brazilian states in both sexes, all ages, and race color. Tobacco consumption in the country is one of the lowest in the world and has declined significantly, which can be attributed to the control policies, regulation, and prevention.


Subject(s)
Humans , Collagen/administration & dosage , Drug Compounding , Extracellular Matrix/metabolism , Mesenchymal Stem Cells/cytology , Cells, Cultured , Mesenchymal Stem Cells/metabolism , Signal Transduction , /metabolism
20.
Rev. bras. enferm ; 68(4): 579-585, jul.-ago. 2015. tab, ilus
Article in Portuguese | LILACS, BDENF | ID: lil-761092

ABSTRACT

RESUMOObjetivo:conhecer concepções de idosos sobre vulnerabilidade ao HIV/Aids e identificar diagnósticos de enfermagem.Método:pesquisa de campo desenvolvida em Unidades de Saúde da Família, João Pessoa. A amostra compreendeu 250 idosos de ambos os sexos com coleta de dados de abril a julho, 2011. Aplicou-se um Teste da Associação Livre de Palavras utilizando o termo: HIV/Aids. Realizou-se análise de conteúdo e mapeamento cruzado dos termos mais frequentes com os da Classificação Internacional para a Prática de Enfermagem, 2011.Resultados:identificaram-se 202 termos, numa frequência total de 1156. Dos 202 termos, 16 foram mais frequentes e utilizados para a construção de diagnósticos de enfermagem. Identificaram-se os diagnósticos conhecimento sobre comportamento sexual adequado, capacidade para proteção parcial, medo da morte e desesperança.Conclusão:compreender essas concepções trouxe conhecimentos acerca de fatores de vulnerabilidades ao HIV/Aids tendo em vista o planejamento de ações de saúde para esse segmento populacional.


RESUMENObjetivo:conocer concepciones de ancianos sobre vulnerabilidad a HIV/Sida y identificar de diagnósticos de enfermería.Método:investigación de campo desarrollado en Unidades de Salud de Familia, João Pessoa. La muestra incluyó 250 ancianos de ambos sexos con recogida de datos de abril a julio, 2011. Se aplicó prueba de asociación de palabras el uso del término: HIV/Sida. Tenido análisis de contenido de proceder cruzar términos más frecuentes con la Clasificación Internacional para la Práctica de Enfermería, 2011.Resultados:202 términos fueron identificados en frecuencia 1156. De los 202 términos, 16 fueron más frecuentes y utilizados para construcción de diagnósticos de enfermería. Se identificaron conocimiento sobre comportamiento sexual apropiado, capacidad de protección parcial, miedo a la muerte y desesperanza.Conclusión:La comprensión de conceptos trajo reflexiones sobre factores de vulnerabilidad ante el HIV/Sida en vista de planificación de acciones la salud para este segmento de población.


ABSTRACTObjective:to know the beliefs of older adults about their vulnerability to HIV/Aids, and to identify nursing diagnoses.Method:a field research implemented in Family Health Units, in João Pessoa, Brazil. The sample included 250 older adults of both genders with data collected from April to July of 2011. A Test of Free Word Association was applied using the term HIV/Aids. A content analysis and cross-mapping of the most frequent terms with the International Classification for Nursing Practice, 2011 were performed.Results:202 terms were identified in terms, with an overall frequency of 1156. Of the 202 terms, 16 were more frequent and were used to construct the nursing diagnoses. The diagnoses identified were knowledge about appropriate sexual behavior, ability for partial protection, fear of death and hopelessness.Conclusion:understanding these beliefs drew from knowledge about factors related to, vulnerability to HIV/Aids aimed at planning health care actions for this population segment.


Subject(s)
Humans , Extracellular Matrix/metabolism , Focal Adhesions , Neoplasms/metabolism , Signal Transduction , Cell Adhesion , Disease Progression , Integrins/metabolism , Models, Biological , Neoplasm Metastasis , Neoplasms/pathology , Neoplasms/therapy
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