ABSTRACT
This study evaluated the effect of diets containing low levels of soluble and insoluble fiber sources on performance, diarrhea score, chemical and physical characteristics of feces, and behavior in weaning piglets. Thirty-six weaned piglets of 30 days of age with an initial body weight of 8.8 kg were distributed in 36 pens using a completely randomized design in an experimental period of 21 days. The experimental treatments were the Control diet (CONT), the Control diet + 1% beet pulp inclusion (SBP), and the Control diet + 1% lignocellulose inclusion (LCE, Arbocel®). Feed and water were available ad libitum. Body weight and feed intake were measured weekly to calculate the average daily intake, weight gain, and feed conversion ratio. The fecal consistency was determined visually twice daily, classifying feces according to three scores. To determine fecal pH and concentration of fecal short-chain fatty acids (SCFA), samples of fresh feces were collected two weeks after weaning and measured by a digital pH meter and gas chromatography, respectively. The behavior of piglets was observed once a week, using four animals per treatment, from 14:00 to 16:00, every 12 min. Fibre sources had no effect (P>0.05) on performance, except in the period 15 to 21 days after weaning, which was a tendency (P=0.061) of feed intake decrease in SBP and LCE diets. Fiber sources did not affect the fecal consistency score (P>0.05). However, piglets fed SBP and LCE showed a tendency (P<0.10) to have less diarrhea incidence 15 to 21 days post-weaning and in the entire experimental period. Fecal pH and SCFA concentration were not influenced by fiber source (P>0.05), with acetic, propionic, and butyric acids representing around 71%, 19%, and 10% of the total, respectively. Fiber sources did not influence the social and feeding behavior of weaning piglets (P>0.05). Diets containing 1% fiber sources did not alter performance, diarrhea score, fecal pH, fecal SCFA concentration, or feeding and social behavior of weaned piglets.(AU)
O estudo avaliou o efeito de dietas contendo baixos níveis de fontes de fibra solúvel e insolúvel sobre o desempenho, escore de diarreia, características químicas e físicas das fezes e comportamento de leitões desmamados. Trinta e seis leitões desmamados, com 30 dias de idade e peso vivo inicial de 8,8 kg, foram distribuídos em 36 baias, totalizando 12 repetições por tratamento, em um delineamento inteiramente casualizado. Os tratamentos experimentais foram: dieta controle (CONT), dieta controle + 1% de inclusão de polpa de beterraba (SBP) e dieta controle + 1% de inclusão de lignocelulose (LCE, Arbocel®). A ração e a água foram disponibilizadas ad libitum durante os 21 dias experimentais. O consumo médio diário de ração (CRM), ganho de peso diário (GPD) e a conversão alimentar (CA) foram medidos semanalmente. A consistência fecal foi determinada visualmente duas vezes por dia, classificando as fezes de acordo com três classificações. Amostras frescas de fezes, colhidas no 14° dia experimental, foram usadas para determinação do pH e ácidos graxos de cadeia curta (AGCC). O pH foi medido utilizando pHmetro digital, enquanto os AGCC foram determinados com auxílio de cromatografia gasosa. Para avaliar o comportamento foram observados quatro leitões por tratamento, uma vez por semana, das 14:00 às 16:00, a cada 12 minutos. As fontes de fibra não apresentaram efeito (P>0,05) sobre as variáveis de desempenho, exceto no período de 15 a 21 dias pós desmame, onde se observou uma tendência (P=0,061) de redução no consumo médio nos leitões que receberam as dietas contendo SBP e LCE. Não foi observado efeito das fontes de fibra sobre o escore de consistência fecal (P>0,05), embora leitões alimentados com SBP e LCE apresentaram uma tendência (P<0,10) de redução na incidência de diarreia no período de 15 a 21 dias pós desmame e no período total. O pH e a concentração de AGCC não foram influenciados pelas fontes de fibra (P>0,05), onde entres os tratamentos os perfis dos ácidos acético, propiônico e butírico foram semelhantes 71%, 19% e 10%, respectivamente. Não houve efeito das fontes de fibra sobre os comportamentos social e alimentar dos leitões (P>0,05). Dietas contendo 1% de fontes de fibra não alteram o desempenho, escore de diarreia, pH fecal, concentração de ácidos graxos voláteis nas fezes, bem como o comportamento alimentar e social de leitões desmamados.(AU)
Subject(s)
Animals , Swine/physiology , Dietary Fiber/analysis , Animal Feed/analysis , Fatty Acids, Volatile/analysis , Food Additives/analysisABSTRACT
OBJECTIVE@#To observe the effects of electroacupuncture at "Siguan" points on behavior, colonic 5-hydroxytryptamine (5-HT) and fecal short-chain fatty acids (SCFAs) in rats with post-stroke depression (PSD), and explore the effect mechanism of electroacupuncture at Siguan points on PSD.@*METHODS@#Fifty SD rats were randomly divided into a sham-operation group, a stroke group, a PSD group, a drug group and an electroacupuncture group, with 10 rats in each one. The stroke model was established by middle cerebral artery occlusion (MCAO) method in the stroke group; except for the sham-operation group, the rats in the other groups were intervened with MCAO combined with solitary and chronic unpredictable mild stress (CUMS) to establish PSD model. In the electroacupuncture group, electroacupuncture was delivered at "Hegu" (LI 4) and "Taichong" (LR 3), with disperse-dense wave, 2 Hz/10 Hz in frequency, for 30 min in each intervention, once daily, for consecutive 21 days. Simultaneously, distilled water (0.01 L•kg-1•d-1) was administrated intragastrically. Fluoxetine solution (2.33 mg•kg-1•d-1) was given by gavage , once a day and for 21 days in the drug group. The same procedure of fixation and gavage with distilled water were adopted in the sham-operation group, the stroke group and the PSD group. Separately, before stroke modeling, after PSD modeling and after 21-day intervention, the consumption of sugar water and the scores of horizontal movement and vertical movement in open-field test were observed. After 21-day intervention, the content of colonic 5-HT was detected by immunohistochemical method, and that of fecal SCFAs was determined by gas chromatography mass spectrometry.@*RESULTS@#After PSD modeling, compared with the stroke group, the sugar water consumption, the horizontal movement scores and vertical movement scores of the open-field test were all reduced in the PSD group, the drug group and the electroacupuncture group (P<0.05). After 21-day intervention, the sugar water consumption and the scores of horizontal movement and vertical movement of the open-field test were increased in the drug group and the electroacupuncture group (P<0.05) when compared with the PSD group; and the horizontal movement score in the electroacupuncture group was lower than that of the drug group (P<0.05). Compared with the sham-operation group, the contents of total fecal SCFAs and acetic acid were lower in the stroke group (P<0.05), and the contents of colonic 5-HT and total fecal SCFAs, acetic acid, propionic acid and butyric acid were reduced in the PSD group (P<0.05). In comparison with the PSD group, the contents of colonic 5-HT and total fecal SCFAs, acetic acid and propionic acid were increased in the drug group and the electroacupuncture group (P<0.05); and the content of colonic 5-HT in the electroacupuncture group was lower than that of the drug group (P<0.05). The level of colonic 5-HT was positively correlated with the contents of total fecal SCFAs and propionic acid (r=0.424, P=0.005; r=0.427, P=0.004).@*CONCLUSION@#Electroacupuncture at "Siguan" points can relieve the depression-like behavior of PSD rats, and its underlying mechanism may be related to the regulation of fecal SCFAs, which affects the release of colonic 5-HT.
Subject(s)
Animals , Rats , Rats, Sprague-Dawley , Propionates , Serotonin , Depression/therapy , Electroacupuncture , Fatty Acids, Volatile , Stroke/complications , Acetic Acid , Butyric Acid , WaterABSTRACT
Genkwa Fols, Kansui Radix, and Euphorbiae Pekinensis Radix in Shizao Decoction(SZD) are toxic to intestinal tract. Jujubae Fructus in this prescription can alleviate the toxicity, but the mechanism is still unclear. Therefore, this study aims to explore the mechanism. To be specific, 40 normal Sprague-Dawley(SD) rats were classified into the normal group, high-dose and low-dose SZD groups, and high-dose and low-dose SZD without Jujubae Fructus(SZD-JF) groups. The SZD groups were given(ig) SZD, while SZD-JF groups received the decoction without Jujubae Fructus. The variation of body weight and spleen index were recorded. The patho-logical changes of intestinal tissue were observed based on hematoxylin and eosin(HE) staining. The content of malondialdehyde(MDA) and glutathione(GSH) and activity of superoxide dismutase(SOD) in intestinal tissue were measured to evaluate the intestinal injury. Fresh feces of rats were collected to detect intestinal flora structure by 16S ribosomal RNA gene(16S rDNA) sequencing technology. The content of fecal short chain fatty acids and fecal metabolites was determined by gas chromatography-mass spectrometer(GC-MS) and liquid chromatography-mass spectrometer ultra-fast liquid chromatography-quadrupole-time-of-flight mass spectrometer(UFLC-Q-TOF-MS), separately. Spearman's correlation analysis was employed to analyze the differential bacteria genera and differential metabolites. RESULTS:: showed that high-dose and low-dose SZD-JF groups had high content of MDA in intestinal tissue, low GSH content and SOD activity, short intestinal villi(P<0.05), low diversity and abundance of intestinal flora, variation in the intestinal flora structure, and low content of short chain fatty acids(P<0.05) compared with the normal group. Compared with high-dose and low-dose SZD-JF groups, high-dose and low-dose SZD groups displayed low content of MDA in intestinal tissue, high GSH content and SOD activity, recovery of the length of intestinal villi, increased abundance and diversity of intestinal flora, alleviation of dysbacteria, and recovery of the content of short chain fatty acids(P<0.05). According to the variation of intestinal flora and fecal metabolites after the addition of Jujubae Fructus, 6 differential bacterial genera(Lactobacillus, Butyricimonas, Clostridia_UCG-014, Prevotella, Escherichia-Shigella, Alistipes),4 differential short chain fatty acids(such as acetic acid, propionic acid, butyric acid, valeric acid) and 18 differential metabolites(such as urolithin A, lithocholic acid, and creatinine) were screened out. Beneficial bacteria such as Lactobacillus were in positive correlation with butyric acid and urolithin A(P<0.05). The pathogenic bacteria such as Escherichia-Shigella were in negative correlation with propionic acid and urolithin A(P<0.05). In summary, SZD-JF caused obvious intestinal injury to normal rats, which could lead to intestinal flora disorder. The addition of Jujubae Fructus can alleviate the disorder and relieve the injury by regulating intestinal flora and the metabolites. This study discusses the effect of Jujubae Fructus in relieving the intestinal injury caused by SZD and the mechanism from the perspective of intestinal flora-host metabolism, which is expected to serve as a reference for clinical application of this prescription.
Subject(s)
Rats , Animals , Rats, Sprague-Dawley , Propionates/pharmacology , Gastrointestinal Microbiome , Fatty Acids, Volatile/pharmacology , Butyrates/pharmacologyABSTRACT
Intestinal flora and its metabolites are closely related to the progression of type 2 diabetes mellitus(T2DM). Eubacterium is one of the dominant intestinal flora, and its metabolites short-chain fatty acids (SCFAs) play a leading role in regulating intestinal metabolic balance. It has been reported that SCFAs can regulate the secretion of glucagon-like peptide-1, improve the function of pancreatic β cells, participate in bile acids metabolism and regulate the production of inflammatory factors in T2DM. Based on the above research background, this article mainly reviews the relationship between Eubacterium and its metabolite SCFAs and T2DM and its regulatory mechanism.
Subject(s)
Humans , Diabetes Mellitus, Type 2 , Eubacterium/metabolism , Fatty Acids, Volatile/metabolism , Gastrointestinal MicrobiomeABSTRACT
BACKGROUND@#Type 2 diabetes mellitus (T2DM) is an independent risk factor for colorectal cancer (CRC), and the patients with CRC and T2DM have worse survival. The human gut microbiota (GM) is linked to the development of CRC and T2DM, respectively. However, the GM characteristics in patients with CRC and T2DM remain unclear.@*METHODS@#We performed fecal metagenomic and targeted metabolomics studies on 36 samples from CRC patients with T2DM (DCRC group, n = 12), CRC patients without diabetes (CRC group, n = 12), and healthy controls (Health group, n = 12). We analyzed the fecal microbiomes, characterized the composition and function based on the metagenomics of DCRC patients, and detected the short-chain fatty acids (SCFAs) and bile acids (BAs) levels in all fecal samples. Finally, we performed a correlation analysis of the differential bacteria and metabolites between different groups.@*RESULTS@#Compared with the CRC group, LefSe analysis showed that there is a specific GM community in DCRC group, including an increased abundance of Eggerthella , Hungatella , Peptostreptococcus , and Parvimonas , and decreased Butyricicoccus , Lactobacillus , and Paraprevotella . The metabolomics analysis results revealed that the butyric acid level was lower but the deoxycholic acid and 12-keto-lithocholic acid levels were higher in the DCRC group than other groups ( P < 0.05). The correlation analysis showed that the dominant bacterial abundance in the DCRC group ( Parvimonas , Desulfurispora , Sebaldella , and Veillonellales , among others) was negatively correlated with butyric acid, hyodeoxycholic acid, ursodeoxycholic acid, glycochenodeoxycholic acid, chenodeoxycholic acid, cholic acid and glycocholate. However, the abundance of mostly inferior bacteria was positively correlated with these metabolic acid levels, including Faecalibacterium , Thermococci , and Cellulophaga .@*CONCLUSIONS@#Unique fecal microbiome signatures exist in CRC patients with T2DM compared to those with non-diabetic CRC. Alterations in GM composition and SCFAs and secondary BAs levels may promote CRC development.
Subject(s)
Humans , Gastrointestinal Microbiome/genetics , Diabetes Mellitus, Type 2 , Microbiota , Bacteria/genetics , Fatty Acids, Volatile , Colorectal Neoplasms/metabolism , Butyrates , Feces/microbiologyABSTRACT
This study aimed to empirically evaluate the adjustment quality of two stoichiometric models of methane production from diets that used different rumen fermentation modulators. We used the models proposed by Moss et al. (2000) and Blümmel et al. (1997). The data set consisted of 169 observations of in vitro methane production and volatile fatty acids (acetate, propionate, and butyrate) from dissertations, theses, and scientific articles. The model's adequacy evaluation was only possible through combination of several statistical analyzes. The models were unable to predict the enteric methane precisely and accurately. Despite this, the model proposed by Moss et al. (2000) showed a better fit based on the mean bias (% of observed, MB), MEF, RMSEP, Cb and CCC measures compared to the model proposed by Blümmel et al. (1997). However, both models presented strong evidence against the general hypothesis H0: a = 0 & b = 1. Thus, the model proposed by MOSS et al. (2000) was more efficient, whereas the model by Blümmel et al. (1997) showed a better fit for diets with high availability of H2.
Subject(s)
Empirical Research , Fatty Acids, VolatileABSTRACT
OBJECTIVES@#To study the influence of enteral feeding initiation time on intestinal flora and metabolites in very low birth weight (VLBW) infants.@*METHODS@#A total of 29 VLBW infants who were admitted to the Department of Neonatology, Children's Hospital of Chongqing Medical University, from June to December, 2020, were enrolled as subjects. According to the enteral feeding initiation time after birth, the infants were divided into two groups: <24 hours (n=15) and 24-72 hours (n=14). Fecal samples were collected at weeks 2 and 4 of hospitalization, and 16S rDNA high-throughput sequencing and gas chromatography-mass spectrometry were used to analyze the microflora and short-chain fatty acids (SCFAs) respectively in fecal samples.@*RESULTS@#The analysis of microflora showed that there was no significant difference between the two groups in Chao index (reflecting the abundance of microflora) and Shannon index (reflecting the diversity of microflora) at weeks 2 and 4 after birth (P>0.05). The analysis of flora composition showed that there was no significant difference in the main microflora at the phylum and genus levels between the two groups at weeks 2 and 4 after birth (P>0.05). The comparison of SCFAs between the two groups showed that the <24 hours group had a significantly higher level of propionic acid than the 24-72 hours group at week 4 (P<0.05), while there was no significant difference in the total amount of SCFAs and the content of the other SCFAs between the two groups (P>0.05).@*CONCLUSIONS@#Early enteral feeding has no influence on the diversity and abundance of intestinal flora in VLBW infants, but enteral feeding within 24 hours can increase the level of propionic acid, a metabolite of intestinal flora.
Subject(s)
Child , Humans , Infant , Infant, Newborn , Enteral Nutrition/methods , Fatty Acids, Volatile , Gastrointestinal Microbiome , Infant, Very Low Birth Weight , Propionates , Prospective StudiesABSTRACT
Halomonas can grow on diverse carbon sources. As it can be used for unsterile fermentation under high-salt conditions, it has been applied as a chassis for next-generation industrial biotechnology. Short-chain volatile fatty acids, including acetate, propionate, and butyrate, can be prepared from biomass and are expected to be novel carbon sources for microbial fermentation. Halomonas sp. TD01 and TD08 were subjected to shaking culture with 10-50 g/L butyrate, and they were found to effectively synthesize poly-3-hydroxybutyrate with butyrate as the carbon source. The highest yield of poly-3-hydroxybutyrate was achieved at butyrate concentration of 20 g/L (9.12 g/L and 7.37 g/L, respectively). Butyrate at the concentration > 20 g/L inhibited cell growth, and the yield of poly-3-hydroxybutyrate decreased to < 4 g/L when butyrate concentration was 50 g/L. Moreover, Halomonas sp. TD08 can accumulate the copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate by using propionate and butyrate as carbon sources. However, propionate was toxic to cells. To be specific, when 2 g/L propionate and 20 g/L butyrate were simultaneously provided, cell dry weight and polymer titer were 0.83 g/L and 0.15 g/L, respectively. The addition of glycerol significantly improved cell growth and boosted the copolymer titer to 3.95 g/L, with 3-hydroxyvalerate monomer content of 8.76 mol%. Short-chain volatile fatty acids would be promising carbon sources for the production of polyhydroxyalkanoates by Halomonas.
Subject(s)
Butyrates , Carbon , Fatty Acids, Volatile , Halomonas , Polyhydroxyalkanoates , PropionatesABSTRACT
Purpose: This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI). Methods: The rats from C. butyricum group were given C. butyricum for 5 days. Then, hepatic ischemia for 30 min and reperfusion for 6 h were performed in all the rats. After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed. Results: The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics. Conclusions: C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress. These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
Subject(s)
Animals , Rats , Reperfusion Injury/veterinary , Protective Agents/administration & dosage , Clostridium butyricum , Fatty Acids, Volatile , Oxidative Stress , Liver Diseases/therapyABSTRACT
La microbiota intestinal es una barrera de defensa natural, encargada de cumplir diversas funciones relacionadas con protección estructural y metabólica al huésped, especialmente contra microorganismos patógenos mediante la competencia de nutrientes y de receptores. Objetivo: describir el vínculo entre la microbiota intestinal y los Acidos Grasos de Cadena Corta en la salud humana. Material y Métodos: se realizó una búsqueda de literatura en bases de datos: PubMed, LILACS y SciELO, Science Direct, Google Académico, Wiley Online Library y Redalyc. Se consideraron un total de 26 documentos, fueron descartados 16 artículos por no cumplir los criterios de inclusión determinados, tener una fecha de publicación menor a 15 años y la pertinencia de su contenido. Se utilizaron los siguientes descriptores en la búsqueda bibliográfica: "Microbiota intestinal", "ácidos grasos de cadena corta", "Short-Chain Fatty Acids", "Gut Microbiota", "Fibra Dietética" y "Dietary Fiber". Conclusión: la ingesta de fibra dietética es fundamental para la integridad de la microbiota intestinal, siendo también importante en la secreción de péptidos antimicrobianos que ejercen función inmunomoduladora, evita la disbiosis y existe una fuerte asociación entre una dieta pobre en fibra y enfermedades desde el inicio de la vida hasta la vejez...(AU)
Subject(s)
Humans , Gastrointestinal Microbiome , Dietary Fiber , Body Powder , Fatty Acids, VolatileABSTRACT
BACKGROUND In order to study the influence of long-term growth process and evolution environment on intestinal bacteria of different breeds, the intestinal bacteria and volatile fatty acids among the faeces of Min, Landrace and Yorkshire pigs were analysed by Illumina high-throughput sequencing of the 16S-rDNA and gas chromatography. RESULTS The shared core microbiota of Landrace, Yorkshire and Min pig were 1273, accounting for 69.56% of total abundance of organisms. The proportion of Firmicutes in Min pig faeces (57.89%) was significantly higher than that in Landrace and Yorkshire pig faeces (47.01% and 46.40%, respectively) (P < 0.05), but that of Bacteroidetes was exactly opposite. Moreover, Min pig presented more highly efficient membrane transport, environmental adaptation, carbohydrate transport, and metabolism than Yorkshire pig (P < 0.05). The acetic acid/total volatile fatty acid ratio in Min pig was significantly higher than that in Landrace pig (P < 0.05), and the isobutyric acid/ total volatile fatty acid ratio in Min pig was significantly larger than that in Yorkshire pig (P < 0.05). Furthermore, the content of branched chain volatile fatty acids in Min pig was significantly higher than that in Yorkshire pig (P < 0.05). CONCLUSION This study demonstrated that Min pig, as an excellent breed in the cold area of China, possessed special intestinal floral structure compared to the imported pigs in order to satisfy their phys iological and metabolic demands, which may influence their characteristics such as resistance to cold, diseases, and crude feeding, and the ability to deposit intramuscular fat.
Subject(s)
Animals , Swine/microbiology , Fatty Acids, Volatile/metabolism , Feces/microbiology , Swine/metabolism , Gastrointestinal MicrobiomeABSTRACT
OBJECTIVES@#To study the effect of the course of treatment with broad-spectrum antibiotics on intestinal flora and short-chain fatty acids (SCFAs) in feces of very low birth weight (VLBW) infants.@*METHODS@#A total of 29 VLBW infants who were admitted to the Neonatal Diagnosis and Treatment Center of Children's Hospital Affiliated to Chongqing Medical University from June to December 2020 were enrolled as subjects for this prospective study. According to the course of treatment with broad-spectrum antibiotics, they were divided into two groups: ≤7 days (@*RESULTS@#There was a significant reduction in Chao index of the intestinal flora in the ≤7 days group and the >7 days group from week 2 to week 4 (@*CONCLUSIONS@#The course of treatment with broad-spectrum antibiotics can affect the abundance, colonization, and evolution of intestinal flora and the content of their metabolites SCFAs in VLBW infants. The indication and treatment course for broad-spectrum antibiotics should be strictly controlled in clinical practice.
Subject(s)
Child , Humans , Infant , Infant, Newborn , Anti-Bacterial Agents , Fatty Acids, Volatile , Feces , Gastrointestinal Microbiome , Infant, Very Low Birth Weight , Prospective StudiesABSTRACT
This study was designed to evaluate the role of short-chain fatty acid butyrate acid on intestinal morphology and function, and atherosclerotic plaque formation in apolipoprotein E-knockout (ApoE
Subject(s)
Animals , Humans , Mice , Apolipoproteins E/genetics , Atherosclerosis/prevention & control , Butyrates/pharmacology , Caco-2 Cells , Diet, High-Fat/adverse effects , Fatty Acids, Volatile , Mice, Inbred C57BL , Mice, Knockout , Plaque, AtheroscleroticABSTRACT
Polyhydroxyalkanoates (PHA) synthesis by activated sludge using volatile fatty acids (VFAs) in fermentation liquid of excess sludge as carbon source is a hotspot in the field of environmental biotechnology. However, there is no unified conclusion on the effects of non-VFAs, mainly dissolved organic matter (DOM), on PHA production. Thus, this critical review mainly introduces the main characteristics and common analysis methods of DOM in anaerobic fermentation liquid. The effects of DOM on PHA production are analyzed from the aspects of microbiology, metabolic regulation and sludge properties. The results of different studies showed that high concentration of DOM is bad for PHA production, but an appropriate amount of DOM is conducive to the stability of sludge properties, reducing the final PHA purification cost. Finally, suitable strategies were proposed to regulate the PHA synthesis by activated sludge with DOM for PHA production by anaerobic fermentation liquid.
Subject(s)
Anaerobiosis , Bioreactors , Fatty Acids, Volatile , Fermentation , Polyhydroxyalkanoates , SewageABSTRACT
Resource utilization is an effective way to cope with the rapid increase of kitchen waste and excess sludge, and volatile fatty acids produced by anaerobic fermentation is an important way of recycling organic waste. However, the single substrate limits the efficient production of volatile fatty acids. In recent years, volatile fatty acids produced by anaerobic co-fermentation using different substrates has been widely studied and applied. In this paper, we analyze the characteristics of fermentation to produce acid using kitchen waste and excess sludge alone or mixture. Influences of environmental factors and microbial community structure on the type and yield of volatile fatty acids in the anaerobic fermentation system are discussed in detail. Moreover, we propose future research directions, to provide a reference for recycling kitchen waste and excess sludge.
Subject(s)
Anaerobiosis , Bioreactors , Fatty Acids, Volatile , Fermentation , Hydrogen-Ion Concentration , Microbiota , Organic Chemicals , SewageABSTRACT
The present study aimed to morphometrically evaluate the ruminal mucosa and ruminal fluid characteristics of Santa Inês sheep fed different levels of spineless cactus as a replacement for Tifton grass hay. A total of 32 intact male Santa Inês sheep, approximately 180 days old and with a mean weight of 32.0±1.6kg, were studied in a completely randomized design with four treatments and eight replicates. The morphometric measurements evaluated in the ruminal epithelium (papilla height and surface area) were significantly affected (P< 0.05) by supplementation of the diet with spineless cactus, and these effects showed increasing linear trends. Evaluations of the ruminal fluid also showed a significant increasing linear effect (P< 0.05) for ammonia and a quadratic effect for microbial protein. The inclusion of spineless cactus in the sheep diet affects the ruminal epithelium morphology, including the papilla height and surface area, and favors the absorption process in the rumen. The diet containing higher levels of spineless cactus led to maximum microbial protein production. These results characterize spineless cactus as a feasible alternative for feeding sheep during periods of drought, when the typical food source is scarce.(AU)
O presente estudo teve como objetivo avaliar morfometricamente a mucosa ruminal e as características do líquido ruminal de ovinos Santa Inês alimentados com diferentes níveis de palma forrageira como substituta do feno de capim-Tifton. Um total de 32 ovinos, machos inteiros, da raça Santa Inês, com aproximadamente 180 dias de idade e peso médio de 32,0±1,6kg, foi estudado em delineamento inteiramente ao acaso, com quatro tratamentos e oito repetições. As medidas morfométricas avaliadas no epitélio ruminal (altura e área superficial da papila) foram significativamente afetadas (P<0,05) pela suplementação da dieta com palma forrageira, e esses efeitos mostraram tendências lineares crescentes. As avaliações do fluido ruminal também mostraram um efeito linear crescente significativo (P<0,05) para a amônia e um efeito quadrático para a proteína microbiana. A inclusão de palma forrageira na dieta de ovinos afeta a morfologia do epitélio ruminal, englobando a altura e a área da papila, e favorece o processo de absorção no rúmen. A dieta contendo níveis mais altos de palma forrageira levou à produção máxima de proteína microbiana. Esses resultados caracterizam a palma forrageira como uma alternativa viável para a alimentação de ovelhas durante períodos de seca, quando a fonte típica de alimento é escassa.(AU)
Subject(s)
Animals , Male , Rumen/anatomy & histology , Sheep/anatomy & histology , Opuntia/chemistry , Mucous Membrane/anatomy & histology , Fatty Acids, VolatileABSTRACT
ABSTRACT Objectives Yacon flour is rich in bioactive compounds (phenolic compounds and fructooligosaccharides (FOS)), and may therefore reduce the risk of diseases associated with excess body weight. However, its effect on fecal short chain fatty acids (SCFA), intestinal permeability, oxidative stress and inflammation markers has not been studied in adult humans with excess body weight. Thus, we evaluated the effect of the consumption of yacon flour on these variables. Materials and methods Twenty-six excess body weight (30.4 ± 2.4 kg/m2) adults (31.3 ± 8.5y) were randomized to one of two groups (yacon flour or control; n = 13) on a double blind clinical trial. Subjects received a breakfast drink containing or not yacon flour (25g) associated with an energy restricted diet, for six weeks. The flour chemical characterization, FOS and total phenolics contents were evaluated. Antioxidant capacity was evaluated in vitro and in vivo (plasma). Intestinal permeability, fecal SCFA, oxidative stress and inflammatory markers also were evaluated in vivo. Results Yacon flour was well tolerated. It presented an in vitro and in vivo antioxidant capacity, increased plasma total antioxidant capacity (ΔYAC: 49.16 (-4.20; 156.63)) and reduced protein carbonyl concentrations (ΔYAC: -0.98 (-1.54; -0.42)). A reduction in SCFAs was observed in both groups (Δacetic: -3.16 (-5.07; -0.95) vs. -1.05 (-2.65; 1.11); Δpropionic: -1.05 (-2.60;-0.38) vs. -0.41 (-2.08; 0.09); Δbutyric: -0.75 (-1.38; -0.04) vs. -0.28 (-0.98; 0.11), for YAC and CON, respectively). Other variables did not change. Conclusion The yacon flour increased the plasma antioxidant capacity, decreased oxidative stress and SCFAs in adults with obesity or overweight.
Subject(s)
Humans , Adult , Asteraceae , Flour , Permeability , Oxidative Stress , Diet , Overweight , Fatty Acids, Volatile , Inflammation , ObesityABSTRACT
BACKGROUND: Salep is obtained by grinding dried orchid tubers and used as a valuable ingredient in the food industry. Because of the glucomannan content of salep, it is thought to have prebiotic potential. However, there is little information in studies concerning the fermentation characteristics and potential prebiotic properties of salep. The objective of this study was to investigate the effect of salep on bifidobacterial growth by measuring the highest optical density (OD), calculating the specific growth rates, and determining the production of lactic acid and short-chain fatty acids (acetic, propionic, and butyric acid) as a result of bacterial fermentation. RESULT: The OD and pH values obtained in this study showed that salep was utilized as a source of assimilable carbon and energy by the Bifidobacterium species (BS). All Bifidobacterium strains produced lactic, acetic, propionic, and butyric acid, indicating that salep is readily fermented by these bacteria. Salep at 1% (w/v) showed a similar effect on bifidobacterial growth as that promoted by 1% (w/v) glucose used as a traditional carbon source. CONCLUSIONS: Bifidobacterium species can develop in media containing salep as well as in glucose and exhibit the potential to be used as new sources of prebiotics.
Subject(s)
Powders/metabolism , Bifidobacterium/growth & development , Bifidobacterium/metabolism , Fatty Acids, Volatile/biosynthesis , Propionates/analysis , Propionates/metabolism , Food Industry , Acetic Acid/analysis , Acetic Acid/metabolism , Lactic Acid/analysis , Lactic Acid/metabolism , Probiotics , Butyric Acid/analysis , Butyric Acid/metabolism , Fatty Acids, Volatile/analysis , Prebiotics , Fermentation , Hydrogen-Ion ConcentrationABSTRACT
PURPOSE: Alterations in the intestinal microbiota in early life affects the development of atopic dermatitis (AD) in humans. This study aimed to further investigate the effects of gut dysbiosis in early life in an ovalbumin (OVA)-induced mouse model of AD. METHODS: The AD mouse model was developed by serial OVA sensitization and mice were treated with an antibiotic cocktail in their drinking water for 2 weeks before primary sensitization. Probiotics (Lactobacillus rhamnosus, 1 × 10⁹ CFU) or 100 µL of fresh fecal supernatant were orally administered daily from 1 week before the first sensitization until the end of the study. RESULTS: The AD mice which received antibiotics had significantly aggravated phenotypes, including clinical score, transepidermal water loss, and histopathology, compared to those treated with healthy feces or probiotics. Total systemic immunoglobulin E production and skin interleukin (IL) 4 levels were significantly increased in the antibiotic-treated mice compared to the other groups. Antibiotic treatment also increased the levels of IL17 and group 3 innate lymphoid cells (ILC3) in the gut and significantly suppressed the production of short-chain fatty acids (SCFAs) and decreased the number FOXP3⁺ cells. CONCLUSIONS: Our results suggest that the status of the gut microbiota in early life in the mouse may play a crucial role in AD development through intestinal SCFA production through regulate the numbers of CD4⁺IL17⁺/CD4⁺FOXP3⁺ regulatory T cells and ILC3s.
Subject(s)
Animals , Humans , Mice , Anti-Bacterial Agents , Cytokines , Dermatitis, Atopic , Drinking Water , Dysbiosis , Fatty Acids , Fatty Acids, Volatile , Feces , Gastrointestinal Microbiome , Immunoglobulin E , Immunoglobulins , Interleukins , Intestines , Lymphocytes , Microbiota , Ovalbumin , Ovum , Phenotype , Probiotics , Skin , T-Lymphocytes, Regulatory , WaterABSTRACT
PURPOSE: The roles of gut microbiota on the natural course of atopic dermatitis (AD) are not yet fully understood. We investigated whether the composition and function of gut microbiota and short-chain fatty acids (SCFAs) at 6 months of age could affect the natural course of AD up to 24 months in early childhood.METHODS: Fecal samples from 132 infants were analyzed using pyrosequencing, including 84 healthy controls, 22 transient AD and 26 persistent AD subjects from the Cohort for Childhood Origin of Asthma and Allergic Diseases (COCOA) birth cohort. The functional profile of the gut microbiome was analyzed by whole-metagenome sequencing. SCFAs were measured using gas chromatography-mass spectrometry.RESULTS: Low levels of Streptococcus and high amounts of Akkermansia were evident in transient AD cases, and low Clostridium, Akkermansia and high Streptococcus were found in children with persistent AD. The relative abundance of Streptococcus positively correlated with scoring of AD (SCORAD) score, whereas that of Clostridium negatively correlated with SCORAD score. The persistent AD group showed decreased gut microbial functional genes related to oxidative phosphorylation compared with healthy controls. Butyrate and valerate levels were lower in transient AD infants compared with healthy and persistent AD infants.CONCLUSIONS: Compositions, functions and metabolites of the early gut microbiome are related to natural courses of AD in infants.