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1.
Braz. j. biol ; 83: e248305, 2023. tab
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1339359

ABSTRACT

Abstract Fertilization with dehydrated sewage sludge can speed up the recovery process of degraded areas due to nutrients concentration, favoring the development of pioneer plants such as Acacia auriculiformis A. Cunn. ex Beth (Fabales: Fabaceae) and the emergence of insects. This study aimed the evaluation of chewing, pollinating insects, predators, their ecological indices and relationships on A. auriculiformis plants fertilized with dehydrated sewage sludge. The experimental design was completely randomized with two treatments (with and without dehydrated sewage sludge) and 24 repetitions. The prevalence of chewing insects Parasyphraea sp. (Coleoptera: Chrysomelidae), Nasutitermes sp. (Blattodea: Termitidae), and Tropidacris collaris (Stoll, 1813) (Orthoptera: Romaleidae), defoliation, and ecological indices of abundance of Coleoptera and Orthoptera were observed on fertilized A. auriculiformis. Acacia auriculiformis plants, with a superior number of branches/tree, revealed greater abundance of Coleoptera and Orthoptera, species richness of pollinating insects, defoliation, numbers of Parasyphraea sp. and T. collaris. The ones with larger leaves/branches displayed greater abundance of species richness of Coleoptera and Diabrotica speciosa (Germar, 1824) (Coleoptera: Chrysomelidae). Therefore, the use of A. auriculiformis plants, fertilized with dehydrated sewage sludge, is promising in the recovery of degraded areas due to the ecological indices increase of chewing and pollinators insects and spiders in the analyzed area.


Resumo A fertilização com lodo de esgoto desidratado pode acelerar o processo de recuperação de áreas degradadas devido à concentração de nutrientes, favorecendo o desenvolvimento de plantas pioneiras tais como Acacia auriculiformis A. Cunn. ex Beth (Fabales: Fabaceae) e de seus insetos. O objetivo deste trabalho foi avaliar os insetos mastigadores, polinizadores e predadores e seus índices e relações ecológicas em plantas de A. auriculiformis fertilizadas com lodo de esgoto desidratado, em área degradada, durante 24 meses. O delineamento foi inteiramente casualizados com dois tratamentos (com e sem adubação com lodo de esgoto desidratado) e 24 repetições (uma repetição = uma planta). O maior número de insetos mastigadores Parasyphraea sp. (Coleoptera: Chrysomelidae), Nasutitermes sp. (Blattodea: Termitidae) e Tropidacris collaris (Stoll, 1813) (Orthoptera: Romaleidae), de desfolha, e do índice ecológico abundância de Coleoptera e de Orthoptera foram maiores em plantas de A. auriculiformis fertilizadas do que nas não fertilizadas com lodo de esgoto desidratado. Plantas de A. auriculiformis, com maior quantidade de galhos/árvore, apresentaram maiores abundâncias de Coleoptera e Orthoptera, riqueza de espécies de insetos polinizadores, desfolha e números de Parasyphraea sp. e T. collaris, e as com maior folhas/galho os de riqueza de espécies de Coleoptera e Diabrotica speciosa (Germar, 1824) (Coleoptera: Chrysomelidae). Por tanto, a utilização de A. auriculiformis, adubada com lodo de esgoto desidratado, é promissora na recuperação de áreas degradadas devido ao aumento dos índices ecológicos de insetos mastigadores, polinizadores e aranhas na área.


Subject(s)
Animals , Acacia , Fabaceae , Sewage , Fertilization , Insecta
2.
Femina ; 49(5): 309-313, 20210531. ilus
Article in Portuguese | LILACS | ID: biblio-1290569

ABSTRACT

Introdução: A gravidez heterotópica é um fenômeno obstétrico muito raro em concepções espontâneas no qual gestações tópica e ectópica coexistem. O diagnóstico é difícil, mas, se realizado precocemente, o prognóstico é favorável. Descrição do caso: Paciente do sexo feminino, de 35 anos de idade, admitida com quadro de dor abdominal e pequeno sangramento vaginal. Diagnosticada precocemente e tratada cirurgicamente por gravidez heterotópica naturalmente concebida. Como resultado, a gravidez tópica seguiu sem intercorrências. Conclusão: Esse caso enfatiza a necessidade de considerar esse diagnóstico diferencial e analisar clínica e ecograficamente as características globais da pelve, mesmo na ausência de fatores de risco em gestações tópicas.(AU)


Introduction: Heterotopic pregnancy (HP) is a rare obstetric phenomenon in spontaneous conceptions in which intrauterine and ectopic pregnancies coexist. The diagnosis is difficult, but, if performed early, the prognosis is favorable. Case description: A 35-year-old woman was admitted with abdominal pain and light vaginal bleeding. She was early diagnosed and surgically treated for a naturally conceived heterotopic pregnancy. As a result, the intrauterine pregnancy went on healthily. Conclusion: This case emphasizes the need to regard HP as a differential diagnosis and analyze the global pelvis characteristics both clinically and in ultrasound scans, even in the absence of risk factors when dealing with intrauterine pregnancies.(AU)


Subject(s)
Humans , Female , Pregnancy , Pregnancy Complications/surgery , Pregnancy Complications/diagnostic imaging , Pregnancy, Tubal/surgery , Pregnancy, Tubal/diagnostic imaging , Pregnancy, Heterotopic/surgery , Pregnancy, Heterotopic/diagnostic imaging , Uterine Hemorrhage/complications , Fertilization
3.
Diagn. tratamento ; 26(1): 35-38, jan.-mar. 2021.
Article in Portuguese | LILACS | ID: biblio-1247984

ABSTRACT

Infertilidade é uma condição que afeta muitos casais. Entre 30% e 40% de mulheres em fertilização assistida apresentam depressão e ansiedade. O relacionamento conjugal também é atingido. Fatores psicossociais que podem tornar indivíduos inférteis mais vulneráveis ao estresse são: características de personalidade, sentimentos de ameaça ou de perda relacionados à infertilidade, percepção de pouco controle sobre a condição de infertilidade e o resultado do tratamento, utilização frequente de estratégias de enfrentamento caracterizadas por evitação e fuga, insatisfação conjugal e dificuldade de comunicação do casal e rede de apoio social insuficiente. Observou-se pequena associação, mas significativa, entre estresse e angústia e a redução das chances de gravidez com o tratamento. A infertilidade e as abordagens terapêuticas para fertilização provocam mudanças na autoestima, no relacionamento e na função sexual. As disfunções sexuais atingem as mulheres (43% a 90%) e os homens (48% a 58%). Além do sofrimento psíquico nas mulheres e em seus parceiros, o comprometimento da vida sexual do casal pode afetar a continuidade do relacionamento, inclusive para o enfrentamento dos desafios do casal que se torna pai e mãe, quando o tratamento é bem-sucedido. A confirmação de que a resiliência pode desempenhar função de proteção nesse percurso, fortalece a importância do acompanhamento psicológico, visando otimizar todos os recursos para melhor superação dessa fase da vida dos casais.


Subject(s)
Anxiety , Mental Health , Sexuality , Fertilization , Infertility
4.
Rev. bras. ginecol. obstet ; 43(1): 54-60, Jan. 2021.
Article in English | LILACS | ID: biblio-1156083

ABSTRACT

Abstract Scientific information on the impact of the new coronavirus (SARS-CoV-2) on the health of pregnant women, fetuses and newborns is considered of limited confidence, lacking good-quality evidence, and drawing biased conclusions. As a matter of fact, the initial impressions that the evolution of COVID-19 was no different between pregnant and non-pregnant women, and that SARS-CoV-2 was not vertically transmitted, are confronted by the documentation of worsening of the disease during pregnancy, poor obstetric outcomes, and the possibility of vertical transmission. The present article aims to compile the data available on the association of COVID-19 and reproductive events, from conception to birth.


Resumo As informações científicas sobre o impacto do novo coronavírus, SARS-CoV-2, na saúde de gestantes, fetos e recém-nascidos são consideradas de confiabilidade limitada, sem evidências de boa qualidade, e levam a conclusões enviesadas. De fato, as impressões iniciais de que a evolução da Covid-19 não era diferente entre mulheres grávidas e não grávidas, e de que o SARS-CoV-2 não era transmitido verticalmente, são confrontadas pela documentação de agravamentos da doença durante a gravidez, resultados obstétricos negativos, e a possibilidade de transmissão vertical. Este artigo tem como objetivo compilar os dados disponíveis sobre a associação entre a Covid-19 e os eventos reprodutivos, desde a concepção até o nascimento.


Subject(s)
Humans , Female , Pregnancy , Pregnancy Complications, Infectious , Prenatal Care , SARS-CoV-2 , COVID-19 , Infectious Disease Transmission, Vertical , Delivery, Obstetric , Fertilization
5.
Protein & Cell ; (12): 7-28, 2021.
Article in English | WPRIM | ID: wpr-880895

ABSTRACT

Mammalian fertilization begins with the fusion of two specialized gametes, followed by major epigenetic remodeling leading to the formation of a totipotent embryo. During the development of the pre-implantation embryo, precise reprogramming progress is a prerequisite for avoiding developmental defects or embryonic lethality, but the underlying molecular mechanisms remain elusive. For the past few years, unprecedented breakthroughs have been made in mapping the regulatory network of dynamic epigenomes during mammalian early embryo development, taking advantage of multiple advances and innovations in low-input genome-wide chromatin analysis technologies. The aim of this review is to highlight the most recent progress in understanding the mechanisms of epigenetic remodeling during early embryogenesis in mammals, including DNA methylation, histone modifications, chromatin accessibility and 3D chromatin organization.


Subject(s)
Animals , Chromatin Assembly and Disassembly , DNA Methylation , DNA Transposable Elements , Embryo, Mammalian , Embryonic Development/genetics , Epigenesis, Genetic , Epigenome , Female , Fertilization/physiology , Gene Expression Regulation, Developmental , Histone Code , Histones/metabolism , Male , Mice , Oocytes/metabolism , Spermatozoa/metabolism
6.
Braz. arch. biol. technol ; 64: e21200071, 2021. tab, graf
Article in English | LILACS | ID: biblio-1278445

ABSTRACT

Abstract Composting of domestic residues to replace cattle manure was evaluated as a fertilization option for the cherry tomato crop. Two sources of organic compounds (CO) were used to prepare compost piles: Domestic residues (DR) and bovine manure (BM), used in 5 proportions (CO1 = 15% DR + 15% BM, CO2 = 10% DR + 20% BM, CO3 = 20% DR + 10% BM, CO4 = 30% BM (control) and CO5 = 30% DR). To compost the compost piles, the organic waste (carbon source) was mixed with remnants of tree pruning (filler) from the urban cleaning service in a ratio of 1: 3. After 90 days, the compost from each matured pile was mixed with 1: 1 coconut fiber substrate and filled into 15 L plastic bags where the cherry tomato plants were grown. The experiment was conducted in a greenhouse using the experimental design of randomized blocks with 5 treatments and 5 replicates (6 fruits per sample). The organic fertilization in the proportions of manure and food residue did not provide statistically significant differences in soluble solids, total sugars, lycopene, β-carotene and micronutrients Zn, Fe, N and P in cherry tomato fruits. Fertilization with CO1 and CO2 increased AT, Mn and decreased the SS / AT, K and Ca ratio. There was no nutritional deficiency of tomatoes in any of the proportions of the organic residues studied.


Subject(s)
Lycopersicon esculentum , beta Carotene , Fertilization , Minerals , Manure
7.
Rev. SPAGESP ; 21(2): 97-110, jul.-dez. 2020. tab
Article in Portuguese | LILACS, INDEXPSI | ID: biblio-1125734

ABSTRACT

A literatura menciona a importância do lazer na vida em família, ressaltando as interações significativas que promove e suas contribuições ao bem-estar da família. Buscou-se compreender a vivência do lazer a partir da perspectiva do grupo familiar, descrevendo as possíveis influências da experiência do lazer na dinâmica das mesmas. Participaram três famílias, que responderam a um questionário sociodemográfico e a uma entrevista do grupo familiar, além de terem produzido registros fotográficos de momentos de lazer. Para análise das entrevistas, incluindo os relatos acerca das fotografias, utilizou-se a Análise de Conteúdo. Verificou-se que as famílias descreveram atividades e concepções de lazer diversas e que todas mencionaram os efeitos positivos que a vivência coletiva do lazer gera às suas dinâmicas, favorecendo a convivência, o diálogo e a alegria. No entanto, as limitações de tempo, dinheiro, de acesso a opções de lazer e de conciliação das demandas de gerações diferentes foram descritos como empecilhos para a vivência familiar do lazer. Conclui-se refletindo acerca das implicações dos achados para políticas públicas de lazer e para uma educação para o lazer.


The literature mentions the importance of leisure in family life, highlighting the significant interactions it promotes and its contributions to family well-being. It sought to understand the experience of leisure from the perspective of the family group, describing the possible influences of the experience of leisure on their dynamics. Three families participated, who answered a sociodemographic questionnaire and an interview of the family group, besides having produced photographic records of leisure moments. For the analysis of the interviews, including the reports about the photographs, the Content Analysis was used. It was verified that the families described various leisure activities and conceptions and that all mentioned the positive effects that the collective experience of leisure generates to their dynamics, favoring the coexistence, the dialogue, and the joy. However, the limitations of time, money, access to leisure options, and the conciliation of the demands of different generations were described as impediments to the family experience of leisure. We conclude by reflecting on the implications of the findings for leisure public policies and leisure education.


En la literatura se menciona la importancia de la recreación en la vida familiar, destacando las importantes interacciones que promueve y sus contribuciones al bienestar de la familia. Trató de comprender la experiencia de recreación desde la perspectiva del grupo familiar, describiendo las posibles influencias de la experiencia de recreación en su dinámica. Participaron tres familias que respondieron a un cuestionario sociodemográfico y a una entrevista del grupo familiar, además de haber producido registros fotográficos de los momentos de recreación. Para el análisis de las entrevistas, incluyendo los informes sobre las fotografías, se utilizó el Análisis de Contenido. Se comprobó que las familias describían diversas actividades y concepciones de recreación y que todas mencionaban los efectos positivos que la experiencia colectiva de recreación genera a sus dinámicas, favoreciendo la convivencia, el diálogo y la alegría. Sin embargo, las limitaciones de tiempo, dinero, acceso a opciones de recreación y la conciliación de las demandas en las diferentes generaciones se describieron como impedimentos para la experiencia familiar de recreación. Concluimos reflexionando sobre las implicaciones de los hallazgos para las políticas públicas de recreación y para la educación del recreación.


Subject(s)
Humans , Male , Female , Quality of Life , Recreation , Family , Family Characteristics , Family Relations , Fertilization , Leisure Activities
8.
Biomédica (Bogotá) ; 40(4): 604-608, oct.-dic. 2020. tab
Article in English | LILACS | ID: biblio-1142426

ABSTRACT

Abstract: Heteropaternal superfecundation is an extremely rare phenomenon that occurs when a second ova released during the same menstrual cycle is additionally fertilized by the sperm cells of a different man in separate sexual intercourse. In August, 2018, the Grupo de Genética de Poblaciones e Identificación at Universidad Nacional de Colombia received a request to establish the paternity of a pair of male twins with genetic markers. The following analyses were performed: amelogenin gene, autosomal short tandem repeat (STR), and Y-STR analyses by means of human identification commercial kits, paternity index, and the probability of paternity calculation and interpretation. A paternity index of 2.5134E+7 and a probability of paternity of 99.9999% for twin 2 were obtained while 14 out of 17 Y-chromosome markers and 14 out of 21 autosomal short tandem repeats were excluded for twin 1. The results indicated that the twins have different biological fathers. Although heteropaternal superfecundation is rarely observed among humans given its low frequency, in paternity disputes for dizygotic twins it is mandatory to demand the presence of the two twins in the testing to avoid wrong conclusions.


Resumen: La superfecundación heteropaternal es un fenómeno extremadamente raro que se produce cuando un segundo óvulo, liberado durante el mismo ciclo menstrual, es fertilizado por un espermatozoide de un hombre diferente en relaciones sexuales separadas. En agosto de 2018, el Grupo de Genética de Poblaciones e Identificación de la Universidad Nacional de Colombia recibió una solicitud para establecer la paternidad mediante marcadores genéticos de un par de mellizos varones, en quienes se hizo el análisis del gen de amelogenina, el análisis de repeticiones cortas en tándem (Short Tandem Repeats, STR) autosómicas y del cromosoma Y (Y-STR) mediante kits comerciales de identificación humana y cálculos e interpretación del índice de paternidad y probabilidad de paternidad. Se obtuvo un índice de paternidad de 2,5134E+7 y una probabilidad de paternidad de 99,9999 % para el gemelo 2, en tanto que en el gemelo 1 se excluyeron 14 de los 17 marcadores del cromosoma Y y 14 de los 21 sistemas STR autosómicos evaluados. Los resultados indicaron que los gemelos tienen diferentes padres biológicos. A pesar de que la superfecundación heteropaternal rara vez se observa en humanos debido a su baja frecuencia, en las disputas de paternidad para los gemelos dicigóticos, es obligatorio exigir en la prueba la presencia de los dos gemelos para evitar conclusiones incorrectas.


Subject(s)
Twins, Dizygotic , Paternity , DNA Fingerprinting , Microsatellite Repeats , Fertilization
9.
Rev. cuba. endocrinol ; 31(3): e221, sept.-dic. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1156397

ABSTRACT

La diabetes mellitus constituye una entidad con una elevada prevalencia que en los últimos años ha tenido una tendencia ascendente, principalmente en población económicamente activa, lo que implica que cada vez existirán más mujeres en edad fértil con esta enfermedad. Se recomienda que estas mujeres reciban un tipo de atención especializada como la atención preconcepcional, tres meses antes de la concepción como mínimo, para coadyuvar al logro de resultados gestacionales favorables. Es conveniente, entonces, que esta actividad clínica sea protocolizada para normalizar y facilitar la práctica profesional en este campo, con el fin de mejorar la calidad de este servicio asistencial. Este constituye el motivo fundamental de la implementación del protocolo de atención preconcepcional en diabetes, en el Centro de Atención al Diabético del Instituto de Endocrinología, que se presenta en este artículo(AU)


Diabetes mellitus is an entity with a high prevalence that in recent years has had an upward trend, mainly in the economically active population, implying that more and more women of childbearing potential with this disease will exist. It is recommended that these women receive a type of specialized care such as preconception care, at least three months before conception, to help achieve favorable gestational outcomes. It is then desirable that this clinical activity can be registered in order to standardize and facilitate professional practice in this field, in order to improve the quality of this care service. This is the fundamental reason for the implementation of the preconceptional care protocol in diabetes, at the Diabetic Patients Care Center of the Institute of Endocrinology, that is presented in this article(AU)


Subject(s)
Humans , Female , Preconception Care/methods , Diabetes Mellitus/epidemiology , Fertilization , Professional Practice
10.
Biosci. j. (Online) ; 36(2): 439-448, 01-03-2020. tab
Article in English | LILACS | ID: biblio-1146270

ABSTRACT

Fertilization management, mainly nitrogen, is one of the factors that most directly affect corn grain yield. Nitrogen dynamics in the soil is quite complex and its main source currently used in corn production, the urea, undergo intense losses in its conventional form, mainly by volatilization and leaching. Thus, the aim of this study was to assess the effect of forms, sources, and times of nitrogen application on the second crop corn. The experiment was conducted in 2016 in the Fundação Chapadão, Chapadão do Sul, MS, Brazil. The sources conventional urea, polymer-coated urea, and foliar N (8 treatments) were used as follows: control (without N addition), conventional urea (single in V3 and split in V3 and V6), polymer-coated urea (single in V3 and split in V3 and V6), conventional urea + foliar N (conventional urea in V6 and foliar in pre-flowering), polymer-coated urea + foliar N (polymer-coated urea in V6 and foliar in pre-flowering), and foliar N (split in V6 and pre-flowering). The variables stem diameter, ear index, ear length, number of grains per row, number of rows per ear, plant height, first ear height, leaf chlorophyll index, leaf N index, leaf values of Ca, Mg, Fe, Zn, Mn, and Cu, 100-grain weight, and grain yield were assessed. Nitrogen application in the form of conventional urea, polymer-coated urea, and foliar N (single or split) for second crop corn does not result in distinct benefits for the crop. Thus, attention should be paid to the commercialization of the product, which has prices established according to nitrogen forms, but without result, for example, in grain yield, which in fact will compose the producer income. The applied nitrogen form and mode of application were positive only to increase the contents of Ca, Mg, Zn, and Mn in the leaves of second crop corn. Ca was favored by the use of conventional urea and split conventional urea, Mg and Mn were only benefited by foliar N application, and Zn was benefited by the use of conventional urea, split conventional urea, conventional urea + foliar N, and polymer-coated urea+foliar N.


O manejo da adubação, principalmente a nitrogenada, é um dos fatores que afetam mais diretamente a produtividade de grãos de milho. A dinâmica do nitrogênio no solo é bastante complexa e a principal fonte atualmente utilizada na produção do milho, a ureia, em sua forma convencional, sofre perdas intensas, principalmente por volatilização e lixiviação. Assim, o objetivo deste trabalho foi avaliar o efeito de formas, fontes e épocas de aplicação de nitrogênio na cultura do milho segunda safra. O experimento foi conduzido no ano de 2016 na Fundação Chapadão, no município de Chapadão do Sul, MS, Brasil. Foram utilizadas as fontes ureia convencional, ureia revestida com polímero e N foliar (8 tratamentos), sendo eles: testemunha (sem adição de N); ureia convencional (todo aplicado no estádio V3) e (parcelada em V3 e V6); ureia com polímero (todo em V3) e (parcelada em V3 e V6); Ureia convencional + N foliar (ureia convencional em V6 e foliar em pré-florada); Ureia com polímero + N foliar (ureia com polímero em V6 e foliar em pré-florada) e N foliar (parcelado em V6 e pré-florada). Foram avaliados o diâmetro de colmo, índice de espiga, comprimento de espiga, número de grãos por fileira, número de fileiras por espiga, altura de plantas, altura de inserção da primeira espiga, índice de clorofila foliar, índice de N foliar, valores foliares de Ca, Mg, Fe, Zn, Mn e Cu, massa de 100 grãos e produtividade de grãos. A aplicação do nitrogênio na forma de ureia convencional, ureia com polímeros e foliar, de forma única ou parcelada, para o milho segunda safra, não resulta em benefícios distintos para a cultura. Assim, deve-se ficar atento a comercialização do produto, que tem preços estabelecidos de acordo com as formas do nitrogênio, mas sem resultado, por exemplo, na produtividade de grãos, que irá de fato compor a renda do produtor. A forma do nitrogênio aplicado e o modo de aplicação foram positivos apenas para aumentar os teores de Ca, Mg, Zn e Mn nas folhas do milho segunda safra. O Ca foi favorecido pelo uso da ureia convencional e convencional parcelada, o Mg e o Mn foram beneficiados apenas pela aplicação de N foliar, enquanto o Zn foi beneficiado pelo uso da ureia convencional, convencionalparcelada, convencional + N foliar e ureia com polímero + N foliar.


Subject(s)
Zea mays , Fertilization , Nitrogen , Urea
11.
Int. braz. j. urol ; 46(1): 116-123, Jan.-Feb. 2020. graf
Article in English | LILACS | ID: biblio-1056368

ABSTRACT

CASE STUDY 40-year-old male patient and 32-year-old female partner, with a history of primary infertility of two years duration. The workup revealed idiopathic mild oligoasthenotheratozoospermia, and no apparent female infertility factors. The couple has failed three intrauterine insemination (IUI) cycles, planning more IUI cycles but also considering in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI).


Subject(s)
Humans , Male , Spermatozoa/pathology , Oxidative Stress , Sperm Injections, Intracytoplasmic/methods , Oligospermia/pathology , Spermatozoa/physiology , Reproducibility of Results , Semen Analysis/methods , Fertilization/physiology
12.
Neotrop. ichthyol ; 18(1): e190042, 2020. graf
Article in English | LILACS, VETINDEX | ID: biblio-1098415

ABSTRACT

Most teleosts are externally fertilizing, with internal fertilization occurring as a relatively rare event. Until now, Euteleosteomorpha is the only teleost cohort known to undergo internal fertilization. In the teleost cohort Otomorpha, it has been recorded the presence of sperm in the ovaries of some species of Characiformes and Siluriformes, but no fertilized eggs have been found so far in the female reproductive tract. It has been presumed that oocytes can be released into the water with associated spermatozoa and only there becomes fertilized, and the term insemination has been used to characterize the strategy adopted by these fish. Here, we present the discovery of the first case of internal fertilization in the teleost cohort Otomorpha, in Compsura heterura (Characiformes: Characidae). In the course of spawning, the eggs form the perivitelline space and the animal and vegetative poles within the ovaries, evidencing oocyte fertilization. The newly spawned eggs then continue to form the animal and vegetative poles and increase the perivitelline space. These eggs are in the zygotic stage. These data indicate that fertilized eggs are only retained for a short period, providing evidence that C. heterura is a zygoparous fish.(AU)


A maioria dos teleósteos são espécies com fecundação externa, sendo a fecundação interna um evento relativamente raro. Até o momento, Euteleosteomorpha é a única coorte de teleósteos conhecida com espécies de fecundação interna. Na coorte de teleósteos Otomorpha, tem sido registrada a presença de esperma nos ovários de algumas espécies de Characiformes e Siluriformes, porém nenhum ovo fecundado foi encontrado até agora no trato reprodutor feminino. Presume-se que os oócitos possam ser liberados na água associados aos espermatozoides e que somente lá são fecundados, e o termo inseminação tem sido empregado para caracterizar a estratégia adotada por esses peixes. Apresentamos aqui a descoberta do primeiro caso de fecundação interna na coorte de teleósteos Otomorpha, em Compsura heterura (Characiformes: Characidae). Durante a desova, os ovos formam o espaço perivitelino e os polos animal e vegetal dentro dos ovários, evidenciando a fecundação interna. Os ovos recém-desovados continuam a formação dos polos animal e vegetal e aumentam o espaço perivitelino. Esses ovos estão na fase zigótica. Estes dados indicam que os ovos fertilizados são retidos por um curto período, fornecendo evidências de que C. heterura é um peixe zigóparo.(AU)


Subject(s)
Fertilization/genetics , Characidae/genetics , Insemination
13.
Braz. j. biol ; 79(3): 438-445, July-Sept. 2019. tab, graf
Article in English | LILACS | ID: biblio-1001465

ABSTRACT

Abstract In this work, the seminal parameters of P. mesopotamicus were evaluated fresh and after cryopreservation, focusing on the sperm variables that affect the rates of fertilization, hatching and post-hatching parameters such as larval survival and morphology. The semen and oocytes from the animals were collected after extrusion, and seminal quality and oocyte fertilization were analyzed. Subsequently, a portion of each semen sample was cryopreserved and, after two days, the oocytes from three new females were fertilized with cryopreserved semen from the males. The analyzes showed that progressive motility, spermatic vigor, motility duration, number of normal sperm and secondary abnormalities were higher in fresh semen than in semen after thawing (P <0.0001). Similarly, fertilization and hatching rates and the percentage of normal and abnormal larvae in fertilized oocytes were higher when fresh semen was used (P <0.0001). The cryopreservation process affected the qualitative parameters of the semen of Piaractus mesopotamicus. The primary abnormality of spermatozoa was the main variable that influenced both fertilization and hatching rates, both in fresh and thawed semen. The second most important variable that influenced, particularly, thawed semen, was the spermatic vigor.


Resumo Neste trabalho, os parâmetros seminais de P. mesopotamicus foram avaliados fresco e após criopreservação, com foco nas variáveis espermáticas ​​que afetam as taxas de fertilização, eclosão e os parâmetros pós-eclosão como a sobrevivência e a morfologia das larvas. Os espermatozoides e os ovócitos dos animais foram coletados após a extrusão, e a qualidade seminal e a fertilização dos ovócitos foram analisados. Posteriormente, uma porção de cada amostra de semen foi criopreservada e, após dois dias, os ovócitos de três novas fêmeas foram fertilizados com semen criopreservado dos machos. As análises mostraram que a motilidade progressiva, o vigor espermático, a duração da motilidade, o número de espermatozoides normais e anormalidades secundárias foram maiores no semen fresco do que no semen após descongelamento (P <0,0001). Da mesma forma, as taxas de fertilização e eclosão e a porcentagem de larvas normais e anormais em ovócitos fertilizados foram maiores quando o semen fresco foi utilizado (P <0,0001). O processo de criopreservação afetou os parâmetros qualitativos do sêmen de Piaractus mesopotamicus . A anormalidade primária dos espermatozoides foi a principal variável que influenciou tanto a taxa de fertilização como a de eclosão, tanto no semen fresco como no semen descongelado. A segunda variável mais importante que influenciou, particularmente, o semen descongelado, foi o vigor espermático.


Subject(s)
Animals , Male , Female , Reproduction , Spermatozoa/physiology , Characiformes/physiology , Cryopreservation/veterinary , Fertilization
14.
Rev. cub. inf. cienc. salud ; 30(2): e1335, abr.-jun. 2019. tab, fig
Article in Spanish | LILACS, CUMED | ID: biblio-1093068

ABSTRACT

Este estudio tuvo como objetivo identificar los principales elementos a considerar en el diseño de un observatorio. Para esto se analizaron 61 observatorios de diversas temáticas, en su gran mayoría del contexto iberoamericano. La metodología empleada se estructuró en tres etapas, que partieron del análisis conceptual y los componentes principales de los observatorios; le siguieron su clasificación y las regularidades. Se plantearon las recomendaciones y los elementos básicos a definir para el diseño de los observatorios, entre los que se encuentran el objetivo, el alcance, los actores, los procesos y las salidas. En estas etapas se utilizan un conjunto de métodos y herramientas del análisis de la información y las redes sociales que contribuyen al estudio de los observatorios(AU)


The purpose of the study was to identify the main elements to be considered when designing an observatory. To achieve such a goal, analysis was conducted of 61 observatories of various topics, most of them from the Ibero-American context. The method used was structured into three stages: conceptual analysis and main components of the observatories, classification and regularities. Recommendations were made and a list of basic elements was provided which should be defined when designing the observatories, among them the objective, scope, actors, processes and outputs. Throughout these stages, use is made of a number of methods and tools for information and social network analysis which contribute to the study of observatories(AU)


Subject(s)
Humans , Elements , Methodology , Fertilization , Goals
15.
Rev. bras. ginecol. obstet ; 41(3): 183-190, Mar. 2019. graf
Article in English | LILACS | ID: biblio-1003542

ABSTRACT

Abstract Considering that myths and misconceptions regarding natural procreation spread rapidly in the era of easy access to information and to social networks, adequate counseling about natural fertility and spontaneous conception should be encouraged in any kind of health assistance. Despite the fact that there is no strong-powered evidence about any of the aspects related to natural fertility, literature on how to increase the chances of a spontaneous pregnancy is available. In the present article, the Brazilian Federation of Gynecology and Obstetrics Associations (FEBRASGO, in the Portuguese acronym) Committee on Endocrine Gynecology provides suggestions to optimize counseling for non-infertile people attempting spontaneous conception.


Resumo Uma vez que mitos e equívocos sobre a procriação natural se espalham rapidamente na era do fácil acesso à informação e às redes sociais, o aconselhamento adequado sobre a fertilidade natural e a concepção espontânea deve ser encorajado em qualquer tipo de assistência à saúde. Apesar do fato de não haver evidências fortes sobre qualquer dos aspectos relacionados à fertilidade natural, existe literatura sobre como aumentar as chances de uma gravidez espontânea. No presente artigo, a Comissão Nacional de Ginecologia Endócrina da Federação Brasileira das Associações de Ginecologia e Obstetrícia (FEBRASGO) oferece sugestões para otimizar o aconselhamento a pessoas que tentam a concepção espontânea, na ausência do diagnóstico de infertilidade.


Subject(s)
Humans , Male , Female , Pregnancy , Adult , Young Adult , Preconception Care , Fertilization/physiology , Ovulation/physiology , Posture , Brazil , Attitude to Health , Smoking/adverse effects , Age Factors , Maternal Age , Paternal Age , Coitus/psychology , Sex Determination Processes/physiology , Counseling , Diet , Lubricants/administration & dosage , Fertility/physiology , Infertility, Female/diagnosis , Middle Aged
16.
Article in English | WPRIM | ID: wpr-785643

ABSTRACT

OBJECTIVE: In vitro maturation (IVM) of immature oocytes can be useful for some infertile patients. In IVM programs, the rates of embryo formation and pregnancy are low. Therefore, it is essential to recognize the main factors involved in regulating oocyte maturation in vitro. The purpose of this study was to investigate the effects of growth differentiation factor 9 (GDF9) and cumulus cell (CC) supplementation in IVM medium on the rates of embryo formation and viability of human blastocysts.METHODS: A total of 80 germinal vesicle oocytes from stimulated cycles underwent an IVM program. The oocytes were divided into four groups, where group I consisted of IVM media only and served as the control, group II consisted of IVM+CCs, group III consisted of IVM+GDF9 (200 ng/mL), and group IV consisted of IVM+CCs+GDF9 (200 ng/mL). Intracytoplasmic sperm injection was performed on the IVM oocytes, and the cleavage embryos that were generated were vitrified. Following thawing, the embryos were cultured for 3 additional days, and the viability rates of the developed blastocysts were determined.RESULTS: The maturation rate of the oocytes did not differ significantly across the four groups. The fertilization rate in group II was significantly higher than that in the control group (76.5% vs. 46.2%). Embryo formation was significantly more frequent in all experimental groups than in the control group, while blastocyst formation did not show significant differences in the three experimental groups compared to the control. The mean viability rates in groups II, III, and IV were 58.16%, 55.91%, and 55.95%, respectively, versus 37.78% in the control group (p<0.05).CONCLUSION: Supplementation of IVM culture media with GDF9 and CCs enhanced the fertilization, embryo formation, and viability rates of blastocysts generated from vitrified cleavage embryos.


Subject(s)
Blastocyst , Culture Media , Cumulus Cells , Embryonic Structures , Fertilization , Growth Differentiation Factor 9 , Humans , In Vitro Techniques , Oocytes , Pregnancy , Sperm Injections, Intracytoplasmic
17.
Article in Korean | WPRIM | ID: wpr-787498

ABSTRACT

BACKGROUND: The aim of this study was to identify the painkillers preferred for self-administration by doctors working at general hospitals in the capital of the Republic of Korea.METHODS: We collected data, using a questionnaire, from 224 doctors working at secondary or tertiary hospitals in the capital of the Republic of Korea from July 1, 2017 to August 31, 2017. The questionnaire included questions on the preferred type of painkiller for each type of pain and the frequency of painkiller intake. Further, we evaluated the participants on the Likert scale to analyze the consideration and cognition of self-administration of painkillers.RESULTS: The doctors in this study tended to state the trade name of the painkillers rather than the generic name. They preferred acetaminophen for headache and nonsteroidal anti-inflammatory drugs for gastrointestinal (GI) pain, dysmenorrhea, toothache, and musculoskeletal pain. In the choice of painkiller for self-administration, they set utmost importance on the effectiveness of the medicine, followed by the potential side effects, physician's prescription, and the pharmacy's recommendation, in that order. The side effects attribute GI complications, hepatotoxicity, drug tolerance, and delayed diagnosis to painkiller use. There were some remarkable differences between surgeons and non-surgeons, men and women, and specialists and trainees in the conception of painkillers and pain control.CONCLUSION: This is the first study worldwide on the trait of the self-administration of painkillers by doctors, which can serve as a useful reference in clinical settings.


Subject(s)
Acetaminophen , Analgesics , Cognition , Delayed Diagnosis , Drug Tolerance , Dysmenorrhea , Female , Fertilization , Headache , Hospitals, General , Humans , Male , Musculoskeletal Pain , Prescriptions , Republic of Korea , Self Administration , Self Medication , Specialization , Surgeons , Tertiary Care Centers , Toothache
18.
Article in English | WPRIM | ID: wpr-740194

ABSTRACT

OBJECTIVE: To evaluate patient perceptions of preoperative reproductive counseling and to evaluate complications and pregnancy outcomes in women who had radical trachelectomy (RT) for early stage cervical cancer. METHODS: Patients who underwent RT from January 1, 2004, through July 31, 2017, and had been cancer free for more than 1 year after RT were eligible; consented patients were sent a 16-item online survey. RESULTS: Of the 58 eligible patients, 39 patients (67%) completed the questionnaire. Eighteen patients (46%) reported receiving reproductive counseling and 26 (68%) reported receiving counseling about pregnancy risks and complications prior to RT, mainly delivered by gynecologic oncologists. Twenty-nine patients (74%) reported having a complication after RT, and cervical stenosis was the most common complication, occurring in 13 patients (33%). Twenty-four patients actively attempted to conceive after RT, and 20 pregnancies were achieved in 13 patients for a pregnancy rate of 54%. Eight pregnancies were spontaneous and 12 required a fertility treatment. There were 5 spontaneous first-trimester miscarriages; 14 of the 20 pregnancies (70%) resulted in live births. The median time to conception was 13.5 months (range, 1–120). CONCLUSION: A significant proportion of women with early stage cervical cancer do not receive adequate reproductive counseling before RT, and many women undergoing RT experience complications that can negatively impact their fertility. We recommend a preoperative consultation with a reproductive endocrinologist for all patients considering RT.


Subject(s)
Abortion, Spontaneous , Constriction, Pathologic , Counseling , Female , Fertility , Fertility Preservation , Fertilization , Humans , Live Birth , Pregnancy , Pregnancy Outcome , Pregnancy Rate , Pregnancy , Trachelectomy , Uterine Cervical Neoplasms
19.
Article in English | WPRIM | ID: wpr-719678

ABSTRACT

Endometriosis is a chronic inflammatory condition that affects fertility and could be toxic to the ovary. Endometrioma per se and surgical interventions for endometrioma significantly reduce the ovarian reserve. Therefore, to prepare for surgical intervention for endometrioma, the high-risk group with decreased ovarian reserve must be considered. There is no evidence to support the use of surgical intervention before in vitro fertilization (IVF) to improve the reproductive outcomes of subsequent IVF in infertile women with advanced-stage endometriosis or endometrioma. As surgical treatment has few benefits, IVF could be recommended immediately for aiding conception in these women. However, the reproductive prognosis of IVF may be worse in the more advanced stages of endometriosis. When dysmenorrhea is severe or when cancer is suspected, surgery prior to IVF may be necessary and justified. When the size of the endometrioma is very large, surgery could be required prior to IVF to facilitate access to follicles during oocyte retrieval or to improve the ovarian response to controlled ovarian stimulation. Prolonged pituitary downregulation in women with surgically diagnosed endometriosis may be helpful to increase the clinical pregnancy rate in subsequent IVF cycles. The purpose of this paper was to review the efficiency and clinical application of the surgical intervention and IVF for infertile women with advanced-stage endometriosis or endometrioma.


Subject(s)
Cystectomy , Down-Regulation , Dysmenorrhea , Endometriosis , Female , Fertility , Fertilization , Fertilization in Vitro , Humans , Infertility , Laparoscopy , Oocyte Retrieval , Ovarian Reserve , Ovary , Ovulation Induction , Pregnancy Rate , Prognosis
20.
Article in English | WPRIM | ID: wpr-742365

ABSTRACT

Leukocytospermia is an ill-defined and poorly understood condition affecting up to 30% of male factor infertility. Current guidelines on leukocytospermia vary significantly, although it has been linked to increased rates of infertility, uncertainty about its clinical significance, diagnosis, and treatment remains. The guidelines are conflicting with sparse data scattered across different specialties and continents. This study aims to compare and contrast available international guidelines and recommendations. In addition to these guidelines, we sought to consolidate the findings of trials over the last several decades. English language articles on human observational studies, retrospective, prospective, clinical trials and randomized control trials were searched for using the following terms: “leukocytospermia, pyospermia, and male infertility.” Articles about treatment and management of leukocytospermia that were published between January 2010 and April 2018 were included, as well as four articles referenced in best practice and guideline statements from urological and andrological associations. Disagreements on this topic are highlighted as some guidelines describe no correlation between leukocytospermia and infertility while others show that treatment leads to improvement of sperm quality by many measures including improved pregnancy rate. Various treatments have been suggested including antibiotics, anti-inflammatory medications, and frequent ejaculation. There is a need for definitive characterization of Leukocytospermia as an infectious or inflammatory marker and a re-evaluation of the leukocyte concentration threshold. Additional studies investigating rates of conception as a measure of outcome are needed, to provide greater level of evidence and generalizability of leukocytopsermia management.


Subject(s)
Andrology , Anti-Bacterial Agents , Diagnosis , Ejaculation , Fertilization , Humans , Infertility , Infertility, Male , Leukocytes , Male , Male , Practice Guidelines as Topic , Pregnancy Rate , Prospective Studies , Retrospective Studies , Semen , Spermatozoa , Uncertainty
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